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Monoclonal antibody against kanamycin was ready, and competitive direct ELISA and immunochromatographic assay were developed using the antibody to detect kanamycin in animal plasma and milk. kanamycin residues in veterinary medicine. Screened positives can be confirmed using a more sensitive laboratory method such as competitive direct ELISA. Therefore, the assays developed in this study could be used to complement each other as well as other laboratory findings. Moreover, instead of slaughtering the animals to obtain test samples, these methods could be applied to determine kanamycin concentration in the plasma of live animals. spp., and spp. [16], and is known to perturb protein synthesis in Gram-negative bacteria by binding to the 30 S subunit of ribosomal RNA, which causes misreading of the genetic code and inhibits translation [6,15]. Kanamycin is a mixture of 3 isomers: kanamycin A, kanamycin B, and kanamycin C. Since the kanamycin components differ markedly in their toxicity, commercial ...
In article ,Pine.SOL.3.96.970711115134.16570A-100000 at ascc.artsci.wustl.edu,, Alex Brands ,abbrands at artsci.wustl.edu, wrote: , I was planning to use kanamycin resistance as a selectable marker in , yeast, and I aquired a construct from another lab that has a kanamycin , resistance cassette. However, my negative control plates revealed that , the parent yeast (w303) was not sensitive to the kanamycin. After talking , to the other lab, I found out that they use something called Geneticin, , (that name is a registered trademark of GIBCO) which is about 20 times , more expensive than kanamycin. , , So, is my kanamycin simply expired, or are yeast not sensitive to , kanamycin? Am I stuck with Geneticin? , , , All help is appreciated very much! , , Alex Brands , Washington University Sorry, youre stuck with the Geneticin (also known as G418). Although the kanamycin resistance factor inactivates both kanamycin and G418, only the latter antibiotic is effective against eukaryotic cells. Steve ...
Monoclonal antibody against kanamycin was ready, and competitive direct ELISA and immunochromatographic assay were developed using the antibody to detect kanamycin in animal plasma and milk. kanamycin residues in veterinary medicine. Screened positives can be confirmed using a more sensitive laboratory method such as competitive direct ELISA. Therefore, the assays developed in this study could be used to complement each other as well as other laboratory findings. Moreover, instead of slaughtering the animals to obtain test samples, these methods could be applied to determine kanamycin concentration in the plasma of live animals. spp., and spp. [16], and is known to perturb protein synthesis in Gram-negative bacteria by binding to the 30 S subunit of ribosomal RNA, which causes misreading of the genetic code and inhibits translation [6,15]. Kanamycin is a mixture of 3 isomers: kanamycin A, kanamycin B, and kanamycin C. Since the kanamycin components differ markedly in their toxicity, commercial ...
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|p||strong|Technical Advantage:|/strong|  Effective against both Gram-positive and -negative bacteria|br /| Kanamycin is a member of the aminoglycosides a group of molecules and interacts with the 30S subunit of prokaryotic ribosomes.  It induces errors in translation and thus hinders protein synthesis.  The KanR-Tn5 gene product (aminoglycoside phosphotransferase) confers resistance to kanamycin.  Kanamycin Sulfate is effective against Gram-negative and -positive bacteria.|br /| -  Complete Certificate of Analysis available for each production lot, along with full formulation information|br /| -  Produced under the highest industry standards to ensure superior results|/p|
Cayetana Schluter wrote: , , Hello all, , , Im trying to do plant (ginseng) transformations and Im using kanamycin , to select for the transformants. Does anybody know how long kanamycin can , remain in solid MS media (50mg/L) at room temperature before it breaks , down? Ginseng is rather slow in that it takes at least 3 weeks before the , tissue starts to callus, so the fewer times I half to transfer my explants , the better. , , Cayetana I routinely do plant tissue culture only in the presence of 20 mg/L of kanamycin and I have found that transfers over 2 week intervals have never been a problem in the selection process. So I am guessing that at 50 mg/L you can at least go for 3 weeks at room temp. provided your Agrobacterium does not start growing back due to a breakdown in the antibiotic you use to select against that. Good luck. VP ...
Kanamycin - Get up-to-date information on Kanamycin side effects, uses, dosage, overdose, pregnancy, alcohol and more. Learn more about Kanamycin
1-N-isoseryl derivatives of kanamycin A, kanamycin B and 3,4-dideoxykanamycin B are now synthesized, which are new and useful compounds active against gram-negative and gram-positive bacteria and also against drug-resistant strains of these bacteria. The production of the 1-N-isoserylkanamycins is made by reacting isoserine with the 1-N-amino group of kanamycin with the functional amino group(s) being protected, following by removal of the amino-protecting groups and by chromatographic separation of the desired 1-N-isoserylation product.
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Definition : Immunoassay reagents intended to perform quantitative analyses on a body fluid sample (e.g., serum, plasma) to measure levels of kanamycin, an aminoglycoside bactericidal agent. Kanamycin is used in the treatment of a wide variety of aerobic gram-negative bacteria and also for some gram-positive bacteria including mycobacteria. Reagents for kanamycin drug level measurement are used to monitor the therapeutic drug level in patients undergoing treatment, either to determine the adequacy of drug treatment or to diagnose a drug overdose or drug-related toxicity. Typical reference levels in blood, in microgram per milliliter, are: peak 20 to 25, predose (trough) 5 to 10, and toxic more than 35.. Entry Terms : "Kanamycin Drug Level Determination Reagents" , "Reagents, Immunoassay, Therapeutic Drug, Antibiotic, Kanamycin". UMDC code : 20040 ...
Name: SALK_063013. ABRC stock number: SALK_063013. Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds ...
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A lateral flow strip biosensor for fast, sensitive, low-cost and on-site detection of kanamycin was developed by using kanamycin-specific aptamer-modified gold nanoparticles (AuNPs-apt) as a probe and oligonucleotide DNA1-modified silver nanoparticles (AgNPs-DNA1) as a signal amplification element. Through t
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Some side effects may occur that usually do not need medical attention. These side effects may go away during treatment as your body adjusts to the medicine. Also, your health care professional may be able to tell you about ways to prevent or reduce some of these side effects. Check with your health care professional if any of the following side effects continue or are bothersome or if you have any questions about them:. ...
Issue Tuberculosis (TB) affects 9 million people and kills 1.4 million annually, mostly in developing countries, and is increasingly resistant to treatment by standard, first-line drugs. Treatment of resistant TB (MDR-TB) costs upwards of $5,000 per patient. Solution The goal of the project was to engineer a yeast organism to make TB drugs from glucose. The process involved applying molecular genetics methodology to transfer all steps of the 2-deoxystreptamine (2-DOS) pathway to S. cerevisiae, thereby constructing new strains of yeast to act as a cell factory for the production of aminoglycosides. The ability of these strains to produce 2-DOS and kanamycin in batch cultures containing either minimal or complex media with 3% glucose was evaluated by TLC (Thin Layer Chromatography). Outcome The results of the TLC assay demonstrated the ability of both yeast strains to produce 2-DOS and kanamycin in only complex media, as compared with the control. However, this method is not reproducible or ...
The bacterial strain was EHA 105, containing a binary vector carrying the GUS gene under control of CaMV35S promoter and NPTII gene under control of the same promoter. Cotyledons from twelve days old E. saligna plantlets were co-cultured for 30 min in the bacterial suspension (OD600nm = 0.5) followed by a 5 day co-culture on MS culture medium containing 2.7 µM NAA + 4.4 µM BAP in the dark. The explants were then transferred on the same medium supplemented with (1) 12.5 mg L-1 kanamycin (Km) + 300 mg L-1 Augmentin (Aug); (2) 25 mg L-1Km+ 300 mg L-1 Aug and (3) 50 mg L-1Km+ 300 mg L-1 Aug. The explants were subcultured in the same culture medium every 15 days and, after 60 days, the percentage of oxidation, callus and shoot formation, and shoot number per explant were evaluated. DNA was extracted from fresh young leaves and processed according to the specific protocol [4]. The presence of GUS gene in the putative transformed plants was confirmed by PCR 10 months after inoculation.Gus expression ...
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... Designation: pBSL97 TypeStrain=False Application: contains easily purifiable cassette(s) for construction aminoglycoside phosphotransferase kanamycin resistance, neomycin resistance
3/18/13 1. The plates labeled Transduced FMJ + KM were removed from the incubator at 3:30 pm and scored. 2. There a appears to be a large amount of microbial growth toward the edges of the plate where there was a smaller concentration of KM. There was no sign of microbial growth in the middle of the plate where the largest amount of KM would be present. There may be a false positive present since the Transduced FMJ was mixed with KM and then plated on LB plates instead of selective plates. 3. The experiment should be redone using KM selected plates to fix the above possible error. 3/21/13 1. A colony of cells were chosen on the 100ml of kanamycin plate. Two designated areas containing a high number of colonies was marked and then 5uL of kanamycin was pipetted onto that area in hopes that the presence of these cells did not represent a false positive. 3/22/13 1. Plates were observed and there was not any change. This is not indicative of anything other than the colonies present are resistant to ...
3/18/13 1. The plates labeled Transduced FMJ + KM were removed from the incubator at 3:30 pm and scored. 2. There a appears to be a large amount of microbial growth toward the edges of the plate where there was a smaller concentration of KM. There was no sign of microbial growth in the middle of the plate where the largest amount of KM would be present. There may be a false positive present since the Transduced FMJ was mixed with KM and then plated on LB plates instead of selective plates. 3. The experiment should be redone using KM selected plates to fix the above possible error. 3/21/13 1. A colony of cells were chosen on the 100ml of kanamycin plate. Two designated areas containing a high number of colonies was marked and then 5uL of kanamycin was pipetted onto that area in hopes that the presence of these cells did not represent a false positive. 3/22/13 1. Plates were observed and there was not any change. This is not indicative of anything other than the colonies present are resistant to ...
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3,4-α-Epoxyneamine and its related aminoglycosidic antibiotic derivatives containing 3,4-α-epoxyneamine moiety in the molecule thereof are now provided, which may be in the form of their amino-protected and partially hydroxyl-protected product and which are useful as intermediates for use in the synthetic production of therapeutically valuable 3-deoxy derivatives of aminoglycosidic antibiotics.
Amikacin is shown for the treatment of diseases brought on by powerless living beings, for example, Gram negative: amikacin is dynamic in vitro against Pseudomonas species, Escherichia coli, Proteus (indolpositivo, indolnegativo)
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All patients received a preoperative bowel cleansing, kanamycin and erythromycin orally within 24 hours prior to surgery, and 1 g of a second-generation cephalosporin IV perioperatively. After surgery, patients were randomized to receive either single-dose prophylaxis one hour after surgery or an additional five doses over two consecutive days. Wounds were inspected daily in the hospital and in the clinic 30 days after surgery. The trial was designed to detect a 10% difference in the incidence of SSIs between groups.. ...
I am trying to transform M15 with my construct , which contains my PCR product in PQE30 vector.I conformed presence of my insert by RE. But facing problem in M15 tansformation step.I used PQE30 only as control to check whether my insert is toxic to the bacteria.I do not find any colony there. M15 competent cells grow well in absence of any antibiotic or in presence of Kanamycin 25 microgm/ml concentration ...
Misc.Comments : Expression vector under control of lambdaPL and temperature sensitive lambda repressor. Medium is 1236 LB plus kanamycin ...
Amikacin is an antibiotic used to treat a wide variety of bacterial infections. It it often employed to treat severe infections resistant to other antibioti
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Amikacin sulfate, a active ingredient of samu AMIKACIN injection, is a semi-synthetic aminoglycosides antibiotics derived from Kanamycin. Amikacin, like other aminoglycoside antibiotics, is a bactericidal agent that exerts its action at the level of bacterial ribosome. Amikacin has been shown to be effective against Gram-positive and Gram-negative bacteria, and many aminoglycoside-resistant strains due to its ability to resist degradation by aminoglycoside inactivating enzymes known to affect Gentamicin, Tobramycin and Kanamycin. Amikacin is a broad spectrum bactericidal antibiotic agent well absorbed following intravenous, subcutaneous or intramuscular injection and rapidly transmitted to skin and soft tissue, so it maintains a high therapeutic blood level. Amikacin has few side effects such as nephrotoxicity and ototoxicity ...
The hipA gene at 33.8 min on the Escherichia coli chromosome controls the frequency of persistence upon inhibition of murein synthesis; for strains bearing hipA+ the frequency is 10(-6), and for hipA- strains the frequency is 10(-2). hip+ has been cloned by selection for a kanamycin resistance determinant at 33.9 min. hipA+ is dominant over hipA- in both recA+ and recA- backgrounds. The smallest DNA insert which contains hipA+, as determined by the ability of the plasmids to complement hipA- strains, is 1,885 base pairs. Both orientations of hipA+ are obtained when the cloning site of vector is remote from strong promoters; both orientations complement hipA-, and both encode a unique peptide of 50,000 Mr. The probable direction of transcription has been deduced from the pattern of peptides encoded by plasmids from which either end of the insert and adjacent vector sequences have been deleted. This information and the recovery of only one orientation of hipA+ when the cloning site is close to a ...
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E. coli BL21-CodonPlus(DE3)-RP transformants were inoculated in 5 mL of Luria-Bertani (LB) medium supplemented with kanamycin 50 μg/mL and cultivated overnight at 37°C. This pre-culture was used to inoculate (1:100) a 100 mL LB medium containing kanamycin, which was cultivated at 37°C until reaching a DO600nm of 0.5-0.6. Then, an aliquot of 10 mL was collected (T0) and IPTG was added. To optimize ArtinM expression, IPTG was tested at different final concentrations (0.01, 0.05, 0.1, 0.2, 0.4, 0.8, 1.0, 1.5, 2.0, 2.5, 3.0, 3.5, 4.0 mM). After the addition of IPTG, each culture was incubated at different temperatures (20, 25, 30 or 37°C), under different shaking speeds (130, 200 or 220 rpm), for different incubation periods (1, 2, 4, 8, 16 and 19 hours). Aliquots (10 mL) were collected at these specific time points to evaluated rArtinM expression. Cells were pelleted by centrifugation at 5,000 × g at 4°C for 5 min, the culture medium was discarded and cells ressuspended in lysis buffer (10 mM ...
The described transformation methods make it possible to generate marker-free transgenic plants with the high frequencies that are required for commercial production. These methods rely on the discovery that a short kanamycin selection phase is as effective as a constant selection regime in arresting the development of wild-type tobacco or potato cells. The presented methods should be generally applicable to plant species amendable to Agrobacterium transformation, especially those not requiring somatic embryogenesis.. While use of transient marker gene expression in transformation is a novel strategy, transient expression of other kinds of genes has long facilitated protein functional screening and promoter analysis (Tai et al., 1999; Thirkettle-Watts et al., 2003). We found a 5-d period adequate for temporary kanamycin selection in Solanaceous species but expect that shorter or longer periods may be equally effective. Modified procedures must neither promote the proliferation of uninfected ...
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ATP, dATP, CTP, ITP and GTP can act as donors; kanamycin, tobramycin and sisomicin can also act as acceptors. The nucleotidyl residue is transferred to the 2-hydroxy of the 3-amino-3-deoxy-D-glucose moiety in the antibiotic ...
... used at adequate concentrations can prevent microbial contamination or act as selection agents to control the expression of specific promotors. Common antibiotics for cultures include ampicillin, actinomycin D, kanamycin or gentamicin.
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Professor Meyer and colleagues, working with GM tobacco plants, have developed a way of splicing into the plant s DNA a gene resistant to the antibiotic kanamycin. After a few generations, the marker gene is bred out of almost half the plants.. The research team believes there is no general reason why this technique shouldn t work with all plant types. Professor Meyer said: When a genetic modification is introduced, only a few cells will integrate the imported DNA. The mechanism we studied is called homologous recombination, where two DNA sequences join together. Once the selected gene is present in the plant, there is no longer a need for the marker. This could be a valuable tool to make genetic modification safer, especially for slow-growing plants such as trees.. For more information, Professor Meyer has a web page ...
민들레의 효과적인 형질전환 방법을 개발하기 위하여 발아 후 6일 된 배축 조직을 reporter 유전자인 EGFP와 kanamycin 저항성 유전자인 npt Ⅱ를 운반하는 Agrobacterium tumefaciens LBA4404에 감염시켰다. 0.5㎎ㆍL⁻½ NAA와 1.0㎎ㆍL⁻½ BA를 첨가한 shoot 유도배지(SI medium)에 2일간 공동 배양한 후 5...