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Ipilimumab, a novel therapy for metastatic melanoma, inhibits cytotoxic T-lymphocyte apoptosis, causing both antitumor activity and significant autoimmunity, including autoimmune thyroiditis. Steroids are frequently used in treatment of immune-related adverse events; however, a concern regarding the property of steroids to reduce therapeutic antitumor response exists. This study describes the first reported case of ipilimumab-associated thyroid storm and implicates iopanoic acid as an alternative therapy for immune-mediated adverse effects. An 88-year-old woman with metastatic melanoma presented with fatigue, anorexia, decreased functional status, and intermittent diarrhea for several months, shortly after initiation of ipilimumab - a recombinant human monoclonal antibody to the cytotoxic T-lymphocyte-associated antigen 4 (CTLA4). On arrival, she was febrile, tachycardic, and hypertensive with a wide pulse pressure, yet non-toxic appearing. She had diffuse, non-tender thyromegaly. An ...
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Browse In Thyroxine (T4), Thyroid function, Thyrotoxicosis, Pyrexia, Triiodothyronine (T3), Gland/Organ, Abdominal pain,...
Ipilimumab, a novel therapy for metastatic melanoma, inhibits cytotoxic T-lymphocyte apoptosis, causing both antitumor activity and significant autoimmunity, including autoimmune thyroiditis. Steroids are frequently used in treatment of immune-related adverse events; however, a concern regarding the property of steroids to reduce therapeutic antitumor response exists. This study describes the first reported case of ipilimumab-associated thyroid storm and implicates iopanoic acid as an alternative therapy for immune-mediated adverse effects. An 88-year-old woman with metastatic melanoma presented with fatigue, anorexia, decreased functional status, and intermittent diarrhea for several months, shortly after initiation of ipilimumab - a recombinant human monoclonal antibody to the cytotoxic T-lymphocyte-associated antigen 4 (CTLA4). On arrival, she was febrile, tachycardic, and hypertensive with a wide pulse pressure, yet non-toxic appearing. She had diffuse, non-tender thyromegaly. An ...
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Project number: 1WEDPP04. Project Synopsis. This project aimed to develop a field liquid delivery system for vaccines or contraceptives being delivered to feral pigs. Critical to ensuring vaccination of feral pigs in the field is consumption of sufficient active liquid. The project initially involved a series of pen trials at Robert Wicks Pest Animal Research Centre (Queensland) to establish iophenoxic acid as a quantitative biomarker.. The aim was to produce a dose response-decay model to which field elevations of blood iodine levels can be compared to assess the volume of vaccine ingested.. Simultaneously, five prototype liquid delivery vehicles were tested on captive feral pigs, to assess uptake of liquid iophenoxic acid and thus the best delivery system in terms of liquid volume consumed.. All blood iodine analysis was performed by the Queensland Department of Natural Resources, Mines and Energy in Brisbane.. The project proceeded to field trials in the state forests near Inglewood, ...
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Body weight regulation: Central and peripheral effects of 3,5,3-triiodothyronine injections in rats<...
TY - JOUR. T1 - Body weight regulation. T2 - Central and peripheral effects of 3,5,3-triiodothyronine injections in rats. AU - Beverly, J. L.. AU - Harris, R. B.S.. AU - Martin, R. J.. PY - 1984/1/1. Y1 - 1984/1/1. UR - http://www.scopus.com/inward/record.url?scp=0021152103&partnerID=8YFLogxK. UR - http://www.scopus.com/inward/citedby.url?scp=0021152103&partnerID=8YFLogxK. M3 - Article. AN - SCOPUS:0021152103. VL - 43. SP - no. 3013. JO - Federation Proceedings. JF - Federation Proceedings. SN - 0014-9446. IS - 4. ER - ...
DIO2 (deiodinase, iodothyronine, type II)
DIO2 (deiodinase, iodothyronine, type II), Authors: Ana Luiza Maia, Simone Magagnin Wajner, Leonardo B Leiria. Published in: Atlas Genet Cytogenet Oncol Haematol.
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中国科学院高能物理研究所机构知识库(IHEP OpenIR): Effect of iodine supplement on iodine status and 5 -deiodinase activity in the brain of...
The weanling Wistar rats of iodine deficiency were divided into three groups for supplementation of different levels of iodine (iodine-excessive [IE], iodine-adequate [IA], and iodine-deficient [ID]), with a control group (C). The iodine content in the thyroid was determined by epithermal neutron activation analysis. The activities of 5-deiodinase and 5-deiodinase in the brains were assayed by determining the conversion ratios of T4 to T3 and rT3, respectively. The thyroid hormones levels in serum were also tested. The results indicated that the ID group had a goiter containing a small amount of iodine, but the IE group had a slightly swollen thyroid with rich iodine; the concentration of iodine per unit mass of thyroid was lower in group IE than in groups IA and C. The highest 5-deiodinase and lowest 5-deiodinase activities in group ID and the lowest 5-deiodinase activity in group IE were found. The iodine deficiency or excess resulted in a compensated hypothyroid state. The results suggest ...
Deiodinase, iodothyronine, type II | definition of deiodinase, iodothyronine, type II by Medical dictionary
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An evidence for the transcriptional regulation of iodothyronine deiodinase 2 by progesterone in ovarectomized rats.
Recent literature lacks studies on the effects of progesterone withdrawal on peripheral conversion of thyroxin (T4) into triiodothyronine (T3) by iodothyronine deiodinase 2 (D2) in different body tissues. The present study aimed to assess the possibl
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Redistribution of deiodinase expression in injured livers. Immunohistochemistry for DIO1 (A) and DIO3 (B) on day 14 after BDL are shown (magnification, ×10 and
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Cardiac expression of human type 2 iodothyronine deiodinase increases by Eun-Gyoung Hong, Brian W. Kim et al.
Altered glucose metabolism in the heart is an important characteristic of cardiovascular and metabolic disease. Because thyroid hormones have major effects on peripheral metabolism, we examined the metabolic effects of heart-selective increase in T3 using transgenic mice expressing human type 2 iodothyronine deiodinase (D2) under the control of the alpha-myosin heavy chain promoter (MHC-D2). Hyperinsulinemic-euglycemic clamps showed normal whole-body glucose disposal but increased hepatic insulin action in MHC-D2 mice as compared to wild-type (WT) littermates. Insulin-stimulated glucose uptake in heart was not altered, but basal myocardial glucose metabolism was increased by more than two-fold in MHC-D2 mice. Myocardial lipid levels were also elevated in MHC-D2 mice, suggesting an overall up-regulation of cardiac metabolism in these mice. The effects of doxorubicin (DOX) treatment on cardiac function and structure were examined using M-mode echocardiography. DOX treatment caused a significant reduction
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Nuclear 3,5,3′-triiodothyronine (T3) in brown adipose tissue: receptor occupancy and sources of T3 as determined by in vivo...
The amount and sources of T3 associated with high affinity, low capacity cellular nuclear receptors in brown adipose tissue (BAT) have been estimated by in vivo pulse-labeling techniques. Maximal binding capacity was measured by in vivo saturation analysis. Nuclear receptor occupancy at endogenous levels of T3 and T4 in euthyroid rats was estimated from the equilibrium nuclear to serum ratio of tracer T3, and the locally generated nuclear T3 to serum T4 ratio after injecting tracer T3 and T4. These ratios were multiplied, respectively, by the endogenous concentrations of T3 and T4 as measured by RIA. The maximal binding capacity was 0.65 ng T3/mg DNA, and saturation was 71%. Fifty-five percent of the nuclear T3 was generated locally, and 45% was derived from circulating T3. BAT is, hence, comparable to the liver in number of receptors (approximately 5000/cell) and to the pituitary with regard to saturation and relative contributions of locally generated T3 and plasma T3 to nuclear T3. These ...
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Safe use of radiographic contrast media
Editor, - I would like to commend Kenneth Thomson and Dinesh Varma for their succinct discussion of the safety profile of iodinated radiographic contrast media (Aust Prescr 2010;33:19-22).. However a noticeable absence in the article is the discussion of oral contrast - particularly the increasing use of injectable iodinated radiographic contrast media as oral contrast (after dilution) for abdominal CT.. One of the issues related to iodinated media like iohexol or diatrizoate sodium is the alleged cumulative nephrotoxicity of these media when given orally in addition to the intravenous dose. This perception appears to be in error. From what I can tell, iohexol is poorly absorbed in the intact gastrointestinal tract and about 1% of the dose is excreted by the kidney. There is however a theoretical potential to cause renal dysfunction in a dehydrated patient as the hypertonic oral iodinated media can cause excessive excretion of water into the gastrointestinal lumen, precipitating a body fluid ...
DIO2 - Wikipedia
Type II iodothyronine deiodinase (iodothyronine 5-deiodinase, iodothyronine 5-monodeiodinase) is an enzyme that in humans is encoded by the DIO2 gene. The protein encoded by this gene belongs to the iodothyronine deiodinase family. It activates thyroid hormone by converting the prohormone thyroxine (T4) by outer ring deiodination (ORD) to bioactive 3,3,5-triiodothyronine (T3). It is highly expressed in the thyroid, and may contribute significantly to the relative increase in thyroidal T3 production in patients with Graves disease and thyroid adenomas. This protein contains selenocysteine (Sec) residues encoded by the UGA codon, which normally signals translation termination. The 3 UTR of Sec-containing genes have a common stem-loop structure, the Sec insertion sequence (SECIS), which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. Alternative splicing results in multiple transcript variants encoding different isoforms. DIO2 has been shown to interact ...
Contrast media 2. CONTRAST MEDIA CHEMICAL PROPERTIES TRIIODINATED COMPOUNDS TRIIODINATED COMPOUNDS BASED ON THE BENZOID ACID...
38 31 y/o male DIES after Myelogram Procedure Myelography is safely performed using nonionic water-soluble radiographic contrast media nonionic water-soluble radiographic contrast media intended for this route of administration intrathecally Misadministration of ionic contrast media intrathecally can result in a syndrome of spasms and convulsions, often leading to death ISOVUE -M ( 20 or 30 cc) ISOVUE -M ( 20 or 30 cc) ...
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The human DIO3 selenoprotein is encoded by the DIO3 gene and catalyzes the inactivation of thyroid hormone by inner ring deiodination of the prohormone thyroxine (T4) and the bioactive hormone 3,3′,5-triiodothyronine (T3) to inactive metabolites, 3,3′,5′- triiodothyronine (RT3) and 3,3′-diiodothyronine (T2), respectively.. We provide the human DIO3 enzyme with approx. 20% Sec contents, mixed with inactive forms containing Sec-to-Lys and Sec-to-Gln substitutions, at more than 95% purity in aliquots at amounts from 50 µg to 500 µg.. ...
Lipopolysaccharide Induces Type 2 Iodothyronine Deiodinase in the Mediobasal Hypothalamus: Implications for the Nonthyroidal...
To determine whether the type 2 iodothyronine deiodinase (D2), the principal central nervous system enzyme converting T4 to biologically active T3, is regulated in tanycytes by im- mune activation, D2 activity was measured in the mediobasal hypothalamus (MBH) 4, 12, and 24 h after administration of bacterial lipopolysaccharide (LPS) and compared with D2 lev- els in the cortex and anterior pituitary of rats. In contrast to D2 activity in the cortex and anterior pituitary that showed a steady linear increase over 24 h, which was coincident with a decline in thyroid hormone and TSH levels, D2 activity peaked in the MBH 12 h after LPS administration. By in situ hybrid- ization, the increased D2 mRNA synthesis induced by LPS was specifically localized to tanycytes lining the third ventricle. In vitro assays in HC11 and HEK-293 cells demonstrated that the p65 subunit of nuclear factor- B markedly increased both rat and human D2 genes (dio2) as analyzed by promoter assays. No activation of human dio2 ...
Immunologic basis for adverse reactions to radiographic contrast media - MELISA
The lymphocyte transformation test (LTT) was used to elucidate whether certain side effects induced by radiographic contrast media have an immunologic etiology. Groups studied were: 8 patients who had previously experienced adverse reactions in association with urography, 6 patients who underwent urography without notable side reactions, 17 occupationally exposed nurses, and 9 unexposed controls. The lymphocytes from 2 hypersensitive patients and from 11 nurses exhibited a positive proliferative response to amidotrizoate. Five nurses who had shown a positive response, had a previous history of hypersensitivity reactions when handling contrast media, whereas the remaining 6 were free of symptoms. Amidotrizoate-specific memory cells were absent in patients who underwent urography without signs of hypersensitivity and in 7/9 of unexposed control subjects. Lymphocytes from patients sensitive to amidotrizoate cross-reacted to structurally related ionic contrast media while non-ionic contrast agents ...
Elsevier: Côté: Clinical Veterinary Advisor, 3rd Edition · Upper Gastrointestinal Radiographic Contrast Series
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iodothyronine sulfotransferase Summary Report | CureHunter
iodothyronine sulfotransferase: hepatic enzyme involved in sulfation of thyroid hormone; SULT1B1 catalyzes the biotransformation of a large number of endogenous compounds such as NEUROTRANSMITTERS, STERIODS, BILE ACIDS, and tTHYROID HORMONES, as well as drugs and XENOBIOTICS; RefSeq NM_014465 (human)
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pH-dependency of iodothyronine metabolism in isolated perfused rat liver | Biochemical Journal
1. Isolated livers from fed male rats were perfused for 2 h with T4 (L-thyroxine), T3 (L-3,3′,5-tri-iodothyronine) or rT3 (L-3,3′,5′-tri-iodothyronine) at different pH values (7.1-7.6) in a fully synthetic medium, whereby normal metabolic functions were maintained without addition of rat blood constituents or albumin. 2. T3 output into the medium and net T3 production reached a maximum at a pH of the medium of 7.2 and significantly decreased with alteration of the pH when livers were perfused with T4 as a substrate. 3. However, the net T4 and T3 uptake by the liver, as well as the hepatic T4 and T3 content after perfusion, were not dependent on the pH of the perfusion when livers were offered T4 or T3 as substrates respectively. 4. Determination of intracellular pH by the analysis of the distribution of the weak acid dimethyloxazolidinedione allows the conclusion that the pH optimum of iodothyronine 5′-deiodinase in the intact perfused liver corresponds to the maximum determined in vitro ...
Patent US4399121 - Iodothyronine immunogens and antibodies - Google Patents
Immunogen conjugates comprising N-aminoalkyl derivatives of iodothyronines, e.g., thyroxine and its lower alkyl esters, coupled to an immunogenic carrier material, and antibodies raised against such immunogen conjugates.
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