Robert K. Strother, Derek B. Danahy, Dmitri I. Kotov, et al. Polymicrobial Sepsis Diminishes Dendritic Cell Numbers and Function Directly Contributing to Impaired Primary CD8 T Cell Responses In Vivo. J. Immunol. 197:4301-4311, 2016.

Figure 2. pDCs undergo cell death during systemic HSV-1 infection in an IFN-I-dependent manner. (A and B) C57BL/6 (A) and IFNAR−/− (B) mice were infected with 107 pfu HSV-1 for 8 h. Control mice were uninfected. pDC frequencies in spleens, expression of PDC-TREM, and caspase activation in pDCs were measured by flow cytometry. Dot plots and histograms show data from three to five mice per genotype representative of three experiments. (C) pDC numbers in spleens from C57BL/6 and IFNAR−/− mice infected or not with 107 pfu HSV-1 for 8 h. Data are representative of three experiments with three to five mice per group. Percentages denote the reduction in pDC numbers in infected mice relative to controls. (D) C57BL/6 mice were treated with an isotype control mAb or anti-IFNAR mAb 4 h before infection with 107 pfu HSV-1. Bar graphs show absolute numbers of pDCs (left) and frequencies of caspase+ pDCs (right) in control and infected mice 8 h p.i. Data are representative of two experiments with ...

CD303 (BDCA-2) antibodies have been used, for example, to identify, characterize, and enumerate plasmacytoid dendritic cells in whole blood of healthy and HIV-infected individuals, and for analyzing the role of DC-SIGN in HIV infection and transmission. Furthermore, CD303 (BDCA-2) antibodies have been used to identify and enumerate plasmacytoid dendritic cells in blood and bone marrow samples before and after hematopoietic stem cell mobilization or transplantation. CD303 (BDCA-2) antibodies have also been used for immunohistochemical staining, for example, to identify plasmacytoid dendritic cells in tissue sections from patients with different inflammatory skin diseases. Clone REA693 recognizes the CD303 (BDCA-2) antigen which is expressed on human plasmacytoid dendritic cells in blood, lymphoid (e.g. tonsils and bone marrow), and non-lymphoid tissue. Specific expression allows direct identification of plasmacytoid dendritic cells using just one marker. CD303 (BDCA-2)+ plasmacytoid dendritic cells in

Plasmacytoid dendritic cell precursors (pDCs) play a key role at the interface of innate and acquired immunity in anti-viral responses by sensing viral infectio...

MACS GMP CpG-P can be used to activate immune cells, in particular human B cells and plasmacytoid dendritic cells (PDCs). - Italia

抗原ペプチドが同定されていない腫瘍に対する細胞傷害性T細胞(CTL)を誘導するために，当研究室で樹立した白血病性形質細胞様樹状細胞株(leukemic plasmacytoid dendritic cell line; PMDC05)の直接

These findings are consistent with the idea that plasmacytoid DCs are mediators of muscle inflammation in juvenile DM. The abundance of CD83+ plasmacytoid DCs in perivascular areas and the overexpression of CCL19 and CCL21 in perivascular cellular foci suggest that in situ activation and maturation …

PREVIOUS PRESENTATION: Portions of this study were presented at the Annual Congress of Société Française dAnesthésie et de Réanimation in Paris, September 2012. BACKGROUND: Toll-like receptor (TLR) agonists are promising therapy for the prevention of nosocomial infections in critical ill patients. We aimed to analyze the TLR-reactivity of circulating dendritic cells (DC) as assessed by cytokine production after an ex vivo challenge with TLR agonists in aneurysmal subarachnoid hemorrhage (SAH) patients. METHODS AND FINDINGS: A single-center prospective observational study took place in one intensive care unit of a teaching hospital. Blood samples were harvested on days 2, 5 and 10 in 21 severe SAH patients requiring mechanical ventilation and 17 healthy controls. DC production of cytokines (Tumour Necrosis Factor, TNF-α; Interleukin, IL-12; and Interferon, IFN-α) was assessed by intracellular immunostaining on TLR-3, 4, 7/8 and 9 stimulations. SAH patients had decreased numbers of blood myeloid

TY - JOUR. T1 - Blastic plasmacytoid dendritic cell neoplasm. T2 - Evolving insights in an aggressive hematopoietic malignancy with a predilection of skin involvement. AU - Gera, Shweta. AU - Dekmezian, Mhair S.. AU - Duvic, Madeleine. AU - Tschen, Jaime A.. AU - Vega, Francisco. AU - Cho-Vega, Jeong Hee. PY - 2014/1/1. Y1 - 2014/1/1. N2 - Blastic plasmacytoid dendritic cell neoplasm (BPDCN) derived from precursors of plasmacytoid dendritic cells is a very rare, unique, and highly aggressive immature hematopoietic malignancy, more frequently occurring among healthy elderly adults. BPDCN can be characterized by a striking predilection for cutaneous involvement, which is often detected incidentally by dermatologists and is difficult to clinically distinguish it from other primary skin lesions and histologically from leukemia/lymphoma cutis. Thus, histological diagnosis of cutaneous biopsies is crucial to correctly classify this entity. Most patients eventually progress to acute myeloid leukemia ...

Immunohistochemical staining revealed a typical blastic plasmacytoid dendritic cell neoplasm (BPDCN) phenotype; the nodules stained positively for CD4, CD56, and CD123, and negatively for CD3, CD8, MPO, TdT, and Epstein-Barr virus. The patient was treated with six cycles of CHOP, and achieved complete remission. Five months later, he presented with a headache and left ptosis. Examination of the cerebrospinal fluid (CSF) showed numerous medium-sized monomorphic cells, either in loose clusters or dispersed singly. The nuclei were round or irregular, showing fine chromatin and prominent nucleoli, and the cytoplasm was scant and grey-blue, with intracytoplasmic microvacuoles and pseudopodia ...

Sigma-Aldrich offers abstracts and full-text articles by [Jordan Ari Schwartz, Kiera L Clayton, Shariq Mujib, Hongliang Zhang, A K M Nur-Ur Rahman, Jun Liu, Feng Yun Yue, Erika Benko, Colin Kovacs, Mario A Ostrowski].

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Blastic plasmacytoid dendritic cell neoplasm (BPDCN) is a particularly aggressive hematologic malignancy with median survival of ,12 months and no standard therapy. BPDCN involves the skin in nearly all patients, and frequently infiltrates bone marrow and lymph nodes. Its normal counterpart may be the plasmacytoid dendritic cell (pDC), leading to the name BPDCN. Outcomes are poor with cytotoxic chemotherapy. An interleukin 3-diphtheria toxin fusion (SL-401) has activity in BPDCN (Frankel, Blood 2014), but additional novel agents are urgently needed. BPDCN over-expresses the anti-apoptotic protein BCL-2 compared to normal pDCs (Sapienza, Leukemia 2014). We confirmed this by RNA-sequencing 12 BPDCNs and pDCs from 4 normal donors (reads/kb mapped [RPKM] 22.7 vs 1.33, P=0.0005). BPDCN shares some genetic characteristics with myeloid malignancies, and some acute myeloid leukemias (AMLs) are dependent on BCL-2. We performed RNA-seq on 6 BPDCN and 16 AML patient-derived xenografts (PDXs) and found ...

On December 21, 2018, the Food and Drug Administration approved tagraxofusp-erzs (ELZONRIS™, Stemline Therapeutics), a CD123-directed cytotoxin, for blastic plasmacytoid dendritic cell neoplasm (BPDCN) in adults and in pediatric patients 2 years and older

Lee-Hwa Tai, Marie-Line Goulet, Simon Belanger, Noriko Toyama-Sorimachi, Nassima Fodil-Cornu, Silvia M. Vidal, Angela D. Troke, Daniel W. McVicar, Andrew P. Makrigiannis ...

I recently (12 Nov 2008) learned that a friend of mine, C.A.M. Wagner, was diagnosed with a rare and aggressive form of bone cancer called plasmacytoid dendritic cell neoplasm. Perhaps only 100 people have received this diagnosis.. C.A.M. and I, along with about 10 others, worked for our fraternity headquarters right out of school. Though we traveled independently to more than 200 universities a semester, we each grew rather close and still keep in touch.. C.A.M., I hope the talented team of doctors and scientist do all they can to make you better.. ...

Plasmacytoid Dendritic Cells in the Duodenum of Individuals Diagnosed with Myalgic Encephalomyelitis Are Uniquely Immunoreactive to Antibodies to...

TORONTO, Sept. 3, 2012- Study reveals possible method of removing leukemia stem cells, preventing relapse of Acute Myeloid Leukemia.

U.S. researchers have found a genetic profile that could identify drugs that harvest the growth of leukemia stem cells. The subpopulation of cells tend to be resistant to cancer therapies, and could even be the root cause of the cancer.

Differential expression of Toll-like receptor (TLR) by conventional dendritic cells (cDCs) and plasmacytoid DC (pDCs) has been suggested to influence the type o

A handful of leukemia cells constantly replenish the supply of cancerous cells, according to new work by Stanford University School of Medicine researchers.