The tuberculin skin test for diagnosing Mycobacterium tuberculosis infection suffers from antigenic cross-reactivity of purified protein derivative with BCG, resulting in poor specificity in BCG-vaccinated populations. Comparative genomics has identified several genetic regions in M. tuberculosis and M. bovis that are deleted in M. bovis BCG. Proteins encoded in these regions will form the basis of new specific T-cell-based blood tests that do not cross-react with BCG, but only two, early secretory antigen target 6 and culture filtrate protein 10, have been studied in detail in humans. We investigated four novel gene products, encoded by RD2 (Rv1989c) and RD1 (Rv3873, Rv3878, and Rv3879c), that are absent from most or all of the vaccine strains of BCG, respectively. Sixty-seven overlapping peptides were tested in ex vivo gamma interferon enzyme-linked immunospot assays in 49 patients with culture-confirmed tuberculosis and 38 healthy BCG-vaccinated donors. Forty-five percent (95% confidence ...
Sepsis is the leading cause of death in the ICU with an estimated 6 million victims per year worldwide. Although septic shock is traditionally viewed as an excessive systemic inflammatory reaction to invasive microbial pathogens, pharmacological suppression of the innate immune response in sepsis has proved to be unsuccessful. An important reason for this might be that the vast majority of septic patients survive the initial pro-inflammatory hit, but die in the subsequent immunosuppressed state due to secondary/opportunistic infections. This so-called immunoparalysis is increasingly recognized as the overriding immune dysfunction in septic patients. Reversal of sepsis-induced immunoparalysis is therefore a promising adjunctive treatment for patients presenting with septic shock.. It was demonstrated that interferon-gamma (IFN)-gamma can reverse immunoparalysis in vitro and in vivo in animals and in healthy volunteers. Moreover, in a case-series of septic patients interferon-gamma treatment ...
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Amoytop Biotech, in collaboration with BioGeneric Pharma, is developing a recombinant human interferon gamma protein drug, for the treatment of an undefined
A biphasic dose-response curve was observed when the IL-1-dependent HTL clone D10 was exposed to IL-1 plus supernatants from some activated T cell clones but not others. The active component that inhibited proliferation at high concentrations of these supernatants appeared to be IFN-gamma based on the following findings: 1) the biphasic pattern of responsiveness correlated with the presence of IFN-gamma in the supernatants; 2) an anti-IFN-gamma mAb augmented the proliferation of D10 cells to these supernatants; 3) rIFN-gamma inhibited profoundly the response of D10 cells stimulated with rIL-1 plus supernatant from activated D10 cells or with rIL-1 plus rIL-4; 4) the response of D10 cells to rIL-1 plus rIL-2 also was inhibited by rIFN-gamma, although to a lesser extent. The proliferation of an additional Th2 clone stimulated with rIL-1 plus rIL-4 or rIL-2 also was inhibited by rIFN-gamma, implicating IFN-gamma as an inhibitory lymphokine for Th2 cells in general. rIFN-gamma did not affect the ...
Interleukin-12 (IL-12) induces differentiation of T helper 1 (Th1) cells, primarily through its ability to prime T cells for high interferon-gamma (IFN-gamma) production. We now report that the presence of IL-12 during the first several days of in vitro clonal expansion in limiting dilution cultures of polyclonally stimulated human peripheral blood CD4+ and CD8+ T cells also induces stable priming for high IL-10 production. This effect was demonstrated with T cells from both healthy donors and HIV+ patients. Priming for IL-4 production, which requires IL-4, was maximum in cultures containing both IL-12 and IL-4. IL-4 modestly inhibited the IL-12-induced priming for IFN-gamma, but almost completely suppressed the priming for IL-10 production. A proportion of the clones generated from memory CD45RO+ cells, but not those generated from naive CD45RO- CD4+ T cells, produced some combinations of IFN-gamma, IL-10, and IL-4 even in the absence of IL-12 and IL-4, suggesting in vivo cytokine priming; ...
Brilliant Violet 510™ anti-human IFN-γ Antibody - Interferon-γ is a potent multifunctional cytokine which is secreted primarily by activated NK cells and T cells.
PubMed comprises more than 30 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
Hochkeppel, H K. and Ley, M D., "Monoclonal antibody against human ifn-gamma." (1982). Subject Strain Bibliography 1982. 2963 ...
Mouse IFN-gamma ELISPOT kit (Black spots, 2-plate (supplied with plates)). The cytokine ELISPOT (Enzyme-Linked ImmunoSPOT) assay is designed to enumerate cytokine-secreting cells in single cell suspensions of lymphoid tissue, central nerve system (CNS) tissue, bone marrow or preparations of peripheral blood mononuclear cells (PBMCs). The assay has the advantage of detecting only activated/memory T cells and has the ability to detect cytokine release in response to antigen by a single cell thereby permitting direct calculation of responder T cell frequencies. The high sensitivity and easy performance, allowing the determination of peptide-reactive T cells without prior in vitro expansion, makes the ELISPOT assay eminently well suited to monitor T cell responses. The higher sensitivity of ELISPOT in comparison to that of ELISA or intracellular staining is due to the plate-bound antibodies directly capturing the cytokine released by the cell before it is diluted in the supernatant, trapped by high-affinity
I just ran across this on the NMSS site and thought that others here would be interested. Researchers Find Clue to Why Women Get MS More Often Than Men An international team of researchers funded in part by the National ...
Monkey IFN-gamma - IL-10 FluoroSpot Kit (2-plate). The dual-color cytokine FluoroSpot assay is a modification of the Enzyme-Linked ImmunoSPOT (ELISPOT) assay and is designed to detect double cytokine-secreting T cells at the single cell level. The assay is based on the use of fluorescent- instead of enzymatic-labeled conjugates. Previous attempts to develop an immunoenzymatic dual-color ELISPOT assay were less successful because of problematic interpretation of mixed color spots. The dual-color FluoroSpot assay, however, provides good discrimination between single and double cytokine-secreting cells and is particularly suited to detect T cell subpopulations with a characteristic cytokine profile. The sensitivity of the assay lends itself to measure very low frequencies of cytokine-secreting T cells (1/300,000).
Recombinant gamma-interferon has activity in chronic myeloid leukemia. American Journal of Clinical Oncology: Cancer Clinical Trials. 1990 ...
Background Production of interferon (IFN)-gamma is key to efficient anti-tumor immunity. The present study was set out to investigate effects of IFNgamma on the release of the potent pro-angiogenic mediator IL-8 by human A549 lung carcinoma cells. Methods A549 cells were cultured and stimulated with interleukin (IL)-1beta alone or in combination with IFNgamma. IL-8 production by these cells was analyzed with enzyme linked immuno sorbent assay (ELISA). mRNA-expression was analyzed by real-time PCR and RNase protection assay (RPA), respectively. Expression of inhibitor-kappaBalpha, cellular IL-8, and cyclooxygenase-2 was analyzed by Western blot analysis. Results Here we demonstrate that IFNgamma efficiently reduced IL-8 secretion under the influence of IL-1beta. Surprisingly, real-time PCR analysis and RPA revealed that the inhibitory effect of IFNgamma on IL-8 was not associated with significant changes in mRNA levels. These observations concurred with lack of a modulatory activity of IFNgamma ...
Interferon-gamma is a regulatory cytokine secreted by activated Th1 Cells which promotes phagocytosis of internalized bacteria by Macrophages. This cytokine is also thought to play an important role in the development of Giant Cells and Granulomas ...
Gessani S, Belardelli F. IFN-gamma expression in macrophages and its possible biological significance. Cytokine & growth factor reviews 1998;9(2):117-123 ...
产品名称:anti-mouseIFN-gammaPurifiedR4-6A2规格:500ug货号:14-7312-85厂商:eBioscience产品介绍:Alsoknownas:Interferon-gamma,IFN-g,IFNgClone:R4-6A2HostRatIsotypeIgG1,kappaReactivityMouseConjugatePurifiedReportedApplica
Induction of IFN-γ- and TNF-α-secreting CD8+ T cell subpopulations upon DC priming.PBMC from volunteer CVD4000#65 were co-cultured with DC alone (media), or p
TY - JOUR. T1 - Interleukin-10 and interferon-gamma gene polymorphisms in patients with nasopharyngeal carcinoma. AU - Farhat, K.. AU - Hassen, E.. AU - Gabbouj, S.. AU - Bouaouina, N.. AU - Chouchane, Lotfi. PY - 2008/6. Y1 - 2008/6. N2 - Nasopharyngeal carcinoma (NPC) is a multifactorial disease. Cytokines driving the immune response seem to be disturbed in NPC patients. Since interleukin-10 (IL-10) is known to reduce the production of interferon-gamma (IFN-γ), we supposed that genetic differences in IL-10 and IFN-γ expression could be a mechanism by which NPC cells escape antitumour immune response. As the production of each cytokine is affected by the genetic background, we investigated the possible association between single nucleotide polymorphisms in genes of IL-10 and IFN-γ with NPC. Different IL-10 -1082 G/A and IFN-γ +874 Τ/Α genotypes were determined in 160 patients with nasopharyngeal carcinoma and 197 healthy controls. No association was found either for each SNP studied alone ...
The International Cytokine & Interferon Society (ICIS), is a non-profit organization of over 950 scientists devoted to research in the fields of cytokine, interferon and chemokine cell biology, molecular biology, biochemistry and the clinical use of these biological response modifiers. Bringing together scientists across many different research disciplines, ICIS is the premier organization promoting the field of cytokine biology, impacting all aspects of medicine and leading to new treatments in autoimmune diseases, cancer, infectious diseases and inflammation. A common ground where scientists interested in all aspect of cytokine biology can join and work together to better human health.. The role of and effect of cytokines in every aspect of human health will continue to be identified and characterized and the use of cytokines themselves or antibodies to cytokines will become even more important tools in the arsenal of clinicians. Thus, the importance of the ICIS as a focal point for cytokine ...
The area of the site you are about to enter is accessible only to Members of the International Cytokine & Interferon Society. In order to proceed, please login or become a member to fully utilize our community platform (click here for more information about membership).. All members should have a login and password to be used to login to the Members Only site, check profile information, renew dues for 2018, receive a registration discount code to save $100 off registration fees ($50 discount for Student/Postdoc registration) to Cytokines 2018 in Boston, and to use the Membership Directory. Members can also apply for ICIS Travel Awards to participate in Cytokines 2018, 27 - 30 October at the Westin Boston Waterfront, Boston, USA as well as nominate others for ICIS prestigious ICIS Awards.. If you have any problems trying to login to the Member Only site, please email the new Managing Director, Joan Oefner.. ...
Activated monocytes play an important role in the pathogenesis of inflammatory arthritis. Blood monocytes which enter the inflamed joint become activated upon adherence to extracellular matrix and exposure to a complex inflammatory environment. We have analyzed the mechanism of monocyte activation by soluble factors present in inflammatory synovial fluid (SF). Greater than 75% of inflammatory SFs tested (a total of 22 fluids to date) increased cell surface expression and dramatically increased mRNA levels of monocyte activation markers Fc gamma RI, Fc gamma RIII, and HLA-DRA. This induction was not triggered by adherence, a known activating stimulus, and several lines of evidence showed that induction was not dependent upon interferon gamma (IFN-gamma). Induction was not prevented by neutralizing anti-IFN-gamma antibodies and IFN-gamma was not detected in the SFs using a sensitive enzyme-linked immunosorbent assay. The SFs also were not able to activate the IFN-gamma-activated transcription ...
Both IL-12 and IFN-gamma have been implicated as principal inducers of type 1 immune responses required for the elimination of intracellular pathogens, such as viruses. We examined the in vivo antiviral role of both cytokines during coronavirus-induced hepatitis in a mouse hepatitis virus (MHV) model. The absence of IFN-gamma function ... read more in mice with a targeted disruption of the IFN-gamma R alpha-chain gene (IFN- gamma R -/-) resulted in increased susceptibility to coronaviral hepatitis associated with augmented viral replication and increased hepatocellular injury. The mutant mice showed a type 1 lymphokine response characterized by the normal high IFN-gamma and low IL-4 production. Unlike MHV-infected wild-type mice, however, the mutant IFN- gamma R -/- mice showed no increase in IL-12 p4O gene expression, similar to that in naive animals. IL-12 treatment failed to restore host resistance in IFN-gamma R -/- mice, but significantly protected MHV-susceptible C57BL/6 mice against ...
ab46604 Human Interferon gamma ELISPOT Kit (Reagents for 10 x 96 tests, without plates) 製品データシート サイトカインの検出に有用 アブカムの ELISPOT キット
Abcam provides general protocols for Human Interferon gamma ELISA Kit (IFNG) (ab108863). Please download our pdf protocol booklet
ab46652 Human Interferon gamma + IL10 FLUOROSPOT Kit 製品データシート サイトカインの検出に有用 アブカムの ELISPOT キット
Interferon-gamma (IFN-gamma) belongs to the type II interferon family and is secreted by activated immune cells-primarily T and NK cells, but also B-cells and APC. INFG exerts its effect on cells by interacting with the specific IFN-gamma receptor (IFNGR). IFNGR consists of two chains, namely IFNGR1 (also known as the IFNGR alpha chain) and IFNGR2 (also known as the IFNGR beta chain). IFNGR1 is the ligand binding receptor and is required but not sufficient for signal transduction, whereas IFNGR2 do not bind IFNG independently but mainly plays a role in IFNG signaling and is generally the limiting factor in IFNG responsiveness. Both IFNGR chains lack intrinsic kinase/phosphatase activity and thus rely on other signaling proteins like Janus-activated kinase 1 (JAK1), JAK2 and Signal transducer and activator of transcription 1 (STAT-1) for signal transduction. IFNGR complex in its resting state is a preformed tetramer and upon IFNG association undergoes a conformational change. This conformational ...
Interferon-gamma (IFN-gamma) belongs to the type II interferon family and is secreted by activated immune cells-primarily T and NK cells, but also B-cells and APC. INFG exerts its effect on cells by interacting with the specific IFN-gamma receptor (IFNGR). IFNGR consists of two chains, namely IFNGR1 (also known as the IFNGR alpha chain) and IFNGR2 (also known as the IFNGR beta chain). IFNGR1 is the ligand binding receptor and is required but not sufficient for signal transduction, whereas IFNGR2 do not bind IFNG independently but mainly plays a role in IFNG signaling and is generally the limiting factor in IFNG responsiveness. Both IFNGR chains lack intrinsic kinase/phosphatase activity and thus rely on other signaling proteins like Janus-activated kinase 1 (JAK1), JAK2 and Signal transducer and activator of transcription 1 (STAT-1) for signal transduction. IFNGR complex in its resting state is a preformed tetramer and upon IFNG association undergoes a conformational change. This conformational ...
Hamster IFN-γ(Interferon gamma) ELISA Kit from ELISA Genie is a pre-coated immunoassay with a sensitivity of 9.375pg/ml and a range of 15.625-1000pg/ml and has been designed to measure Hamster IFN-γ(Interferon gamma) ELISA Kit in serum, plasma & cell culture supernatant samples. The Hamster IFN-γ(Interferon gamma) ELISA Kit accurately measures natural Hamster IFN-γ(Interferon gamma) levels quantified versus standard curves obtained and is based on antibodies raised against a recombinant Hamster IFN-γ(Interferon gamma) peptide.
PubMed comprises more than 30 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
Murrays recent review (1) provides a welcome discussion of the role of interferon-gamma as an immune activator of macrophages against microbial pathogens. A small section of it, however, must respectfully be challenged. Contrary to what Murray suggests, interferon-gamma apparently cannot protect against tubercle bacilli or Mycobacterium avium in humans. Knowledge of this important fact can help terminate an interpolative idea that has been contradicted by well-controlled experimental evidence.. In our several years of research on many variations of an in-vitro model of human tuberculosis infection, using macrophages and virulent tubercle bacilli, we have never found interferon-gamma protective (2). In fact, ...
Abcams Interferon gamma ELISPOT Kit Colorimetric (ab62899) is a highly sensitive cell-based ELISA for the detection of Interferon gamma in stimulated cells.
Our knowledge of the induction of new molecules by IFN-gamma has led to the characterization of IP-10 and the preparation of a monospecific, polyclonal antibody. Using this reagent we have now examined inflammatory states occurring in human skin and used immunocytochemical staining for the expression of both Ia and IP-10 determinants. After evoking a delayed-type response to purified protein derivative of tuberculin (PPD), we noted the presence of IP-10 in dermal macrophages and endothelial cells. Intense staining of the basal layer of epidermal keratinocytes was prominent at 41 h, and by 1 wk the entire epidermis was staining. The comparison of the amount of IP-10 secreted by keratinocytes vs. macrophages, fibroblasts, and endothelial cells revealed that keratinocytes were by far the major producers of this molecule. The expression of Ia occurred in conjunction with IP-10. The injection of rIFN-gamma mimicked many of the features of the PPD response, including the expression of both Ia and ...
Demographic information provided for all subjects who were eligible for the study after all screening procedures were performed. 7 subjects who were found to be ineligible after randomization but prior to administration of treatment (listed as did not meet continuation criteria in participant flow section) are not included in baseline analysis ...
Тези приймаються до 2019-06-01. Конференція проводиться у Vienna, Австрія, з 2019-10-20 по 2019-10-23.
Interferon gamma-induced protein 10 (IP-10), or CXCL10, is a chemokine secreted by monocytes, endothelial cells and fibroblasts in response to interferon gamma (IFN-ɣ).
Results IFN-λ was detected in 76 (29,1%) patients and IFN-α in 115 (44%). In 108 patients (41.4%) neither of the cytokines was detected. Both IFN-λ and IFN-α were detected more often and in higher levels in patients. IFN-λ levels did not correlate to the levels of IFN-α, and only in 38 (14.5%) patients had detectable levels of both. Levels of IL-17, IL-23 and IP-10 correlated with each other and with IFN-λ. In comparison, only a weak correlation between levels of IFN-α and IL-23 was observed. Detectable levels of IFN-λ were associated with lower incidence of fever, photosensitivity and also arthritis. Patients with detectable IFN-α had active mucocutaneous disease, serositis and lower levels of C3 and C4, and also presence of anti-Ro/SSA and anti-La/SSB. Incidence of nefritis and DVT was lower in this group. Co-detection of IFN-α and IFN-λ was significantly associated with lymphadenopathy, serositis, cortical dysfunction and presence of antiphospolipid antibodies. IFN-α was ...
interferon gamma receptor: distinct from the receptors for interferons alpha and beta; RefSeq NM_000416 (human), NM_010511 (mouse)
Author: Praefcke, G. J. K. et al.; Genre: Other; Published in Print: 2000; Title: Identification of regions in human guanylate binding protein 1 (hGBP1) responsible for oligomerization and GTPase activity
This kit is ideal for users who want a convenient and sensitive assay. The assay is designed for the enumeration of cells secreting human IFN-γ. The kit includes ELISpot 8-well strip plates pre-coated with monoclonal antibody, biotinylated detection antibody, Streptavidin-HRP and ready-to-use TMB substrate. A polyclonal activator for human T cells (mAb CD3-2) is supplied as a positive control for cytokine secretion.. Pre-coated plates reduce assay time and minimize variability. In addition, strip-plates are ideal when running a limited number of wells.. Human IFN-γ ELISpotPLUS kits are also available with Streptavidin-ALP and BCIP/NBT-plus substrate. See Related products.. ...
This kit is ideal for users who want a convenient and sensitive assay. The assay is designed for the enumeration of cells secreting human IFN-γ. The kit includes ELISpot plates pre-coated with monoclonal antibody, biotinylated detection antibody, Streptavidin-ALP and ready-to-use BCIP/NBT-plus substrate. A polyclonal activator for human T cells (mAb CD3-2) is supplied as a positive control for cytokine secretion.. Pre-coated plates reduce assay time and minimize variability.. Human IFN-γ ELISpotPLUS kits are also available with Streptavidin-HRP and TMB substrate. See Related products.. ...
The IUPHAR/BPS Guide to Pharmacology. IFN-γ ligand page. Quantitative data and detailed annnotation of the targets of licensed and experimental drugs.
Clone REA600 recognizes the human interferon gamma (IFN-γ) antigen, a cytokine of type II class interferons. IFN-γ is produced by lymphocytes that are involved in inflammatory immune responses. It functions as a potent activator of macrophages, increases the antiviral and antitumor effects of the type I interferons, and has antiproliferative effects on transformed cells. IFN-γ is predominantly released by memory and effector CD4+ and CD8+ T cells as well as by NK cells upon activation. Additional information: Clone REA600 displays negligible binding to Fc receptors. - USA
Its important that the color of white be consistently neutral at all light levels from darkest to brightest. Grayscale performance impacts color accuracy with regard to the secondary colors: cyan, magenta, and yellow. While you can manipulate them wi...
Objectives. Interferon-β (IFN-β) is used in the treatment of multiple sclerosis (MS). IFN-β activation of signal transduction and activation of transcription (STAT)-4 is linked to its immunomodulatory effects. Previous studies suggest a type I IFN deficit in immune cells of MS patients, but data on interferon-α/β receptor (IFNAR) expression and the relationship with treatment response are conflicting. Here we compare IFN-β-mediated STAT4 activation in immune cells of untreated MS patients and controls ...
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Breaking News. A science team have released preliminary news that they can now very easily replicate proof of Cold Fusion / LENR with easy to get material.
LAA049Hu82, FITC-Linked Monoclonal Antibody to Interferon Gamma (IFNg), 干扰素γ(IFNg)单克隆抗体(异硫氰酸荧光素标记), IFN-G; IFG; IFI; INFr; IFN, Immune Interferon | 仅供体外研究使用,不用于临床诊断!请索取进口关税税单及报关单!
APA049Hu61, Active Interferon Gamma (IFNg), 干扰素γ(IFNg)活性蛋白, IFN-G; IFG; IFI; INFr; IFN, Immune Interferon | 仅供体外研究使用,不用于临床诊断!请索取进口关税税单及报关单!