TY - JOUR. T1 - Stimulation of PC cell-derived growth factor (Epithelin/Granulin precursor) expression by estradiol in human breast cancer cells. AU - Lu, Runqing. AU - Serrero, Ginette. PY - 1999/3/5. Y1 - 1999/3/5. N2 - PC cell-derived growth factor (PCDGF) is an 88 kDa glycosylated protein isolated from a highly tumorigenic mouse teratoma derived cell line which is similar to the epithelin/granulin precursor. Using Northern blot and western blot analyses, we detect the expression of PCDGF mRNA and protein in MCF-7 human breast cancer cells. We show that 17-β-estradiol stimulates PCDGF mRNA and protein expression in a time and dose-dependent manner. The stimulation of PCDGF expression by 17-β-estradiol was observed as early as 4 hours and reached a maximum at 12 hours. Maximal stimulation of PCDGF mRNA and protein expression by 17-β-estradiol was observed at a concentration of 10-8 M. The stimulation of PCDGF expression by 17-β-estradiol was completely inhibited by treatment with ...

TY - JOUR. T1 - Solution structure and heparin interaction of human hepatoma-derived growth factor. AU - Sue, Shih Che. AU - Chen, Jeou Yuan. AU - Lee, Shao Chen. AU - Wu, Wen Guey. AU - Huang, Tai-huang. PY - 2004/11/5. Y1 - 2004/11/5. N2 - Hepatoma-derived growth factor (HDGF)-related proteins (HRPs) comprise a new protein family that has been implicated in nephrogenesis, tumorigenesis, vascular development, cell proliferation, and transcriptional activation. All HRPs share a conserved N-terminal homologous to the amino terminus of HDGF (HATH) domain, but vary significantly in the C-terminal region. Here, we show that in solution the N and C termini of human HDGF form two structurally independent domains. The 100 amino acid residue N-terminal HATH domain is well-structured while the 140 amino acid residue C-terminal domain is disordered. We determined the solution structure of the HATH domain by NMR. The core structure of the HATH domain is a five-stranded β-barrel followed by two α-helices, ...

PC cell-derived growth factor (PCDGF), also called epithelin/granulin precursor (GEP), is an 88-kDa secreted glycoprotein with the ability to stimulate cell proliferation in an autocrine fashion. In addition, some studies indicated that PCDGF participated in invasion, metastasis and survival of cancer cells by regulating cell migration, adhesion and proliferation. Yet the effects of PCDGF on proliferation and invasion of ovarian cancer cells in vitro and the mechanisms by which PCDGF mediates biological behaviors of ovarian cancer have rarely been reported. In the present study we investigated whether and how PCDGF/GEP mediated cell proliferation and invasion in ovarian cancer. PCDGF/GEP expression level in three human ovarian cancer cell lines of different invasion potential were detected by RT-PCR and western blot. Effects of inhibition of PCDGF expression on cell proliferation and invasion capability were determined by MTT assay and Boyden chamber assay. Expression levels of cyclin D1 and CDK4 and

Hepatoma-derived growth factor (HDGF) is a growth factor related to normal development and tumorigenesis; however, the mechanism of its mitogenic and angiogenic activity still remains unknown. Analysis of the HDGF interactome could be important for understanding its function and integrative mechanisms, because knowledge about HDGF interactors is very limited. In this study, through streptavidin-binding peptide (SBP) and Flag tag-based tandem affinity purification (SBP/Flag-TAP) coupled with LC-MS/MS, 106 proteins were shown to form complexes with HDGF. RNAs were also found in the HDGF complex through the SBP-tag based RNA co-immunoprecipitation (SBP-RIP) assay. Some of these interactions were confirmed by Co-IP and RT-PCR. We then found that the HATH domain was essential for HDGF interactions including protein-protein and protein-RNA interactions, and that in the absence of the HATH domain, NO-HATH could not form complex. The interactome suggests that HDGF is a multifunctional protein and participates

Hepatoma-derived growth factor (HDGF) belongs to a polypeptide family containing five additional members called HDGF related proteins 1-4 (HRP-1 to -4) and Lens epithelial derived growth factor. Whereas some family members such as HDGF and HRP-2 are expressed in a wide range of tissues, the expression of others is very restricted. HRP-1 and -4 are only expressed in testis, HRP-3 only in the nervous system. Here we investigated the expression of HDGF, HRP-2 and HRP-3 in the central nervous system of adult mice on the cellular level by immunohistochemistry. In addition we performed Western blot analysis of various brain regions as well as neuronal and glial cell cultures. HDGF was rather evenly expressed throughout all brain regions tested with the lowest expression in the substantia nigra. HRP-2 was strongly expressed in the thalamus, prefrontal and parietal cortex, neurohypophysis, and the cerebellum, HRP-3 in the bulbus olfactorius, piriform cortex and amygdala complex. HDGF and HRP-2 were found to be

Hepatoma-derived growth factor (HDGF) belongs to a polypeptide family containing five additional members called HDGF related proteins 1-4 (HRP-1 to -4) and Lens epithelial derived growth factor. Whereas some family members such as HDGF and HRP-2 are expressed in a wide range of tissues, the expression of others is very restricted. HRP-1 and -4 are only expressed in testis, HRP-3 only in the nervous system. Here we investigated the expression of HDGF, HRP-2 and HRP-3 in the central nervous system of adult mice on the cellular level by immunohistochemistry. In addition we performed Western blot analysis of various brain regions as well as neuronal and glial cell cultures. HDGF was rather evenly expressed throughout all brain regions tested with the lowest expression in the substantia nigra. HRP-2 was strongly expressed in the thalamus, prefrontal and parietal cortex, neurohypophysis, and the cerebellum, HRP-3 in the bulbus olfactorius, piriform cortex and amygdala complex. HDGF and HRP-2 were found to be

TY - JOUR. T1 - Inhibition of epidermal growth factor-like growth factor secretion in tracheobronchial epithelial cells by vitamin A. AU - Miller, Lisa. AU - Cheng, Ling Zhong. AU - Wu, Reen. PY - 1993/6/1. Y1 - 1993/6/1. N2 - Vitamin A deficiency of respiratory tract epithelium results in the phenomenon of squamous cell metaplasia. The mechanisms by which vitamin A regulates airway epithelial cell growth and differentiation are not completely understood. In this study, we focused on the effects of vitamin A (retinol) on growth of human and non-human primate tracheobronchial epithelial (TBE) cells in culture. Retinol and its derivatives have little growth-stimulatory effect on TBE cells that are maintained in primary culture in a serum-free medium supplemented with 6 hormonal supple-ments: insulin, transferrin, epidermal growth factor (EGF), hydrocortisone, cholera toxin, and bovine hypothalamus extract. However, it was observed that retinol exhibited dose-dependent inhibition of TBE cell growth ...

Evidence suggests flow-induced arterial remodeling involves factors released from cells that are intrinsic to the vessel wall and recruited from the bloodstream. Understanding the molecular details has been hampered by the need to study the process in vivo. The present findings suggest that HB-EGF, which has primarily been studied in epithelial and tumor cells, plays a pivotal role in low FINR of the mouse carotid artery. Sustained low flow activated or increased the following elements within the HB-EGF signaling pathway: ROS, the ROS-sensitive HB-EGF sheddase TACE, expression of pro-HB-EGF, HB-EGF immunoreactivity, the HB-EGF receptor EGFR, ERK1/2, and the transcription factor NF-κB. These changes were associated with proliferation, increased leukocyte density, wall hypertrophy, and lumen narrowing. Heterozygous and homozygous deletion of HB-EGF alleles caused dose-dependent-like inhibition of FINR (although inhibition of lumen narrowing was in some situations spared (see below), where ...

The growth factor progranulin (acrogranin/PC-derived growth factor/granulin-epithelin precursor) promotes onset of blastocyst cavitation and is required for neonatal hypothalamic sexual differentiation. Little is known, however, of the range of devel

Accumulation of T lymphocytes has been demonstrated in atherosclerotic lesions, and T-cell-derived factors appear to modulate atherosclerotic progression.1 2 3 4 5 6 The present in vitro study demonstrates that lyso-PC can selectively upregulate the expression of HB-EGF and IL-2 receptors in T lymphocytes and suggests that this polar phospholipid increase in inflammatory and atherosclerotic lesions27 28 may be an important stimulus for T cells in atherogenesis in vivo.. Recent studies have demonstrated that human T lymphocytes isolated from peripheral blood can synthesize HB-EGF.6 Expression of HB-EGF has been shown in macrophages and SMCs in atherosclerotic lesions,20 and T lymphocytes isolated from atherosclerotic plaques have been shown to produce HB-EGF.29 Lyso-PC, therefore, may be a relevant stimulus to induce the expression of HB-EGF in atherosclerotic lesions, in addition to other pathophysiological stimuli, such as tumor necrosis factor,30 thrombin,31 and platelet-activating factor.32 ...

cDNA, FLJ94334, Homo sapiens growth arrest-specific 2 (GAS2), mRNA (Growth arrest-specific 2, isoform CRA_a) contains a PF00307 domain.. cDNA, FLJ94334, Homo sapiens growth arrest-specific 2 (GAS2), mRNA (Growth arrest-specific 2, isoform CRA_a) contains a PF02187 domain.. cDNA, FLJ94334, Homo sapiens growth arrest-specific 2 (GAS2), mRNA (Growth arrest-specific 2, isoform CRA_a) is proteolytically cut by caspase-7 (C14.004) cleavage. SRVD-GKTS.. cDNA, FLJ94334, Homo sapiens growth arrest-specific 2 (GAS2), mRNA (Growth arrest-specific 2, isoform CRA_a) is proteolytically cut by caspase-3 (C14.003) cleavage. SRVD-GKTS.. ...

Hepatoma derived growth factor Hepatoma-derived growth factor (HDGF) also known as high mobility group protein 1-like 2 (HMG-1L2) is a protein that in humans is encoded by the HDGF gene. Hepatoma-derived growth factor (HDGF), a potential predictive and prognostic marker in several human cancers, is the firstly reported

Osteoblast differentiation is a pivotal event in bone formation. Runt-related transcription factor-2 (Runx2) is an essential factor required for osteoblast differentiation and bone formation. However, the underlying mechanism of Runx2-regulated osteogenic differentiation is still unclear. Here, we explored the corresponding mechanism using the C2C12/Runx2Dox subline, which expresses Runx2 in response to doxycycline (Dox). We found that Runx2-induced osteogenic differentiation of C2C12 cells results in a sustained decrease in the expression of heparin-binding EGF-like growth factor (HB-EGF), a member of the epidermal growth factor (EGF) family. Forced expression of HB-EGF or treatment with HB-EGF is capable of reducing the expression of alkaline phosphatase (ALP), a defined marker of early osteoblast differentiation. HB-EGF-mediated inhibition of ALP depends upon activation of the EGFR and the downstream extracellular signal-regulated kinase, c-Jun N-terminal kinase mitogen-activated protein kinase

The MET inhibitor INC-280 restored sensitivity to erlotinib and promoted apoptosis in nonCsmall-cell lung cancer choices rendered resistant to erlotinib by hepatocyte growth factor. to revive awareness to erlotinib and promote apoptosis in NSCLC versions rendered erlotinib resistant by HGF. These data give a preclinical rationale for a continuing phase 1 scientific trial of erlotinib plus INC-280 in mutation, among the first identified systems of EGFR TKI level of resistance involves activation from the MET receptor, resulting in restored downstream signaling in both phosphatidylinositol 3-kinase (PI3K)/proteins Rabbit polyclonal to EGFR.EGFR is a receptor tyrosine kinase.Receptor for epidermal growth factor (EGF) and related growth factors including TGF-alpha, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30 and vaccinia virus growth factor. kinase B (AKT) and mitogen-activated proteins kinase/extracellular signal-regulated kinase kinase (MEK)/extracellular ...

TY - JOUR. T1 - Regulation of heparin-binding EGF-like growth factor by miR-212 and acquired cetuximab-resistance in head and neck squamous cell carcinoma. AU - Hatakeyama, Hiromitsu. AU - Cheng, Haixia. AU - Wirth, Pamela. AU - Counsell, Ashley. AU - Marcrom, Samuel R.. AU - Wood, Carey Burton. AU - Pohlmann, Paula R.. AU - Gilbert, Jill. AU - Murphy, Barbara. AU - Yarbrough, Wendell G.. AU - Wheeler, Deric L.. AU - Harari, Paul M.. AU - Guo, Yan. AU - Shyr, Yu. AU - Slebos, Robbert J.. AU - Chung, Christine H.. PY - 2010. Y1 - 2010. N2 - Background: We hypothesized that chronic inhibition of epidermal growth factor receptor (EGFR) by cetuximab, a monoclonal anti-EGFR antibody, induces up-regulation of its ligands resulting in resistance and that microRNAs (miRs) play an important role in the ligand regulation in head and neck squamous cell carcinoma (HNSCC). Methodology/Principal Findings: Genome-wide changes in gene and miR expression were determined in cetuximabsensitive cell line, SCC1, and ...

PubMed comprises more than 30 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.

The present invention relates to the use of fibroblast growth factor-binding protein (FGF-BP) polypeptides, and functional variants of these polypeptides, respectively, or of nucleic acids encoding th

GRN Full-Length MS Protein Standard (NP_002078), Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine, was produced in human 293 cells (HEK293) with fully chemically defined cell culture medium to obtain incorporation efficiency at Creative-Proteomics. Granulins are a family of secreted, glycosylated peptides that are cleaved from a single precursor protein with 7.5 repeats of a highly conserved 12-cysteine granulin/epithelin motif. The 88 kDa precursor protein, progranulin, is also called proepithelin and PC cell-derived growth factor. Cleavage of the signal peptide produces mature granulin which can be further cleaved into a variety of active, 6 kDa peptides. These smaller cleavage products are named granulin A, granulin B, granulin C, etc. Epithelins 1 and 2 are synonymous with granulins A and B, respectively. Both the peptides and intact granulin protein regulate cell growth. However, different members of the granulin protein family may act as inhibitors, stimulators, or have dual

Previously we discovered that the growth arrest-specific gene 6 (in oocyte maturation and fertilization using RNA interference (RNAi). but not nuclear maturation and 2) the decreased manifestation and decreased MPF activity separately or mutually influence sperm head decondensation and PN formation. Intro Mammalian oocytes in ovarian follicles have arrested growth and a large nucleus known as a germinal vesicle (GV). The meiotic cell cycle is arrested in the diplotene stage PDK1 inhibitor of the 1st prophase and some selective oocytes initiate growth following gonadotropin simulation [1] [2]. Oocyte growth and maturation are long and requisite processes for fertilization and subsequent embryo development until embryonic genome activation starts. Oocyte maturation entails nuclear and cytoplasmic maturation. Although strictly linked these are complex and different events [3] [4] [5]. The process of nuclear maturation meiotic cell cycle involves GV breakdown (GVBD) chromosome condensation and ...

HB-EGF is an EGF related growth factor that signals through the EGF receptor, and stimulates the proliferation of smooth muscle cells (SMC), fibroblasts, epithelial cells, and keratinocytes. HB-EGF is expressed in numerous cell types and tissues, including vascular endothelial cells and SMC, macrophages, skeletal muscle, keratinocytes, and certain tumor cells. The ability of HB-EGF to specifically bind heparin and heparin sulfate proteoglycans is distinct from other EGF-like molecules, and may be related to the enhanced mitogenic activity, relative to EGF, that HB-EGF exerts on smooth muscle cells. The human HB-EGF gene encodes a 208 amino acid transmembrane protein, which can be proteolytically cleaved to produce soluble HB-EGF. Recombinant mouse HB-EGF produced in E. coli is a single, non-glycosylated polypeptide chain of 86 amino acids (63-148 a.a.) and a molecular mass of 9.8 kDa. It has bee purified by proprietary chromatographic ...

It was reported that tumor-expressed dickkopf-related (DKK) proteins affect micro-environment. However, the influence of DKK1 on colorectal cancer (CRC) liver oligometastases (CRCLOM) remains unclear. CRC cases after resection of liver oligometastases were enrolled in Sun Yat-Sen University Cancer Center with intact clinical data. Serum DKK1 was detected by ELISA assay. Immunofluorescent staining examination for CD3 and CD8 in slices were also conducted. Among 65 patients included, the recurrence-free survival (RFS) and overall survival (OS) were significantly better in the low serum DKK1 group (RFS: P = 0.021; OS: P = 0.043). DKK1 was overexpressed in stage IV CRC patients in TCGA data. The number of CD8+ tumor-infiltrating lymphocytes (TILs) in invasive margin of CRC liver oligometastases was significantly higher in low serum DKK1 group (P = 0.042). Elevated serum DKK1 level was associated with poorer RFS and OS, and less CD8+ TILs in invasive margin in CRC liver oligometastases. DKK1 might serve as a

HDGFRP2 - (untagged)-Human cDNA FLJ14447 fis, clone HEMBB1001331, weakly similar to Mus musculus mRNA for hepatoma-derived growth factor available for purchase from OriGene - Your Gene Company.

Adipose tissue plays an important role in energy balance by secretion of various adipokines. Obesity is excessive fat accumulation in the body. The aim of this study is to examine the association of apelin, an adipokine secreted from fat tissue, with antioxidant system, glucose and lipid parameters in obese and control cases. After approval has been taken from the Ethics Committee, 61 obese and 24 control people were included in the study. The ages in the study group ranges between 18 and 75. The body mass index (BMI) is ,24.9 in obese people, and it ranges between 18.5-24.9 in control cases. Apelin was calculated using the ELISA method, total oxidant and total antioxidant levels were calculated using the colorimetric method, fasting blood glucose, insulin, TG, LDL and HDL levels were calculated using the photometric method, insulin resistance was calculated using the HOMA-IR method. Serum apelin levels were not found to differ between obese and control groups (p,0.05). Total antioxidant level ...

The dual luciferase reporter system was then used to validate the target genes described in Fig 7. To determine whether bta-let-7i and bta-miR-2305 can directly target their predicted target genes, including MAP3K1, proheparin -binding epidermal growth factor-like growth factor (HBEGF), serine/threonine-protein kinase (PAK1) and Ras-related C3 botulinum toxin substrate 2 (RAC2), we designed luciferase reporter constructs that included either the wild- or mutant-type 3-UTR of MAP3K1, HBEGF, PAK1 and RAC2 (Fig 7C). [score:8] ...

Heparin-binding protein which binds to FGF2, prevents binding of FGF2 to heparin and probably inhibits immobilization of FGF2 on extracellular matrix glycosaminoglycans, allowing its release and subsequent activation of FGFR signaling which leads to increased vascular permeability ...

The inotropic and vasoactive properties of the ligand apelin and its cognate G-protein coupled receptor APJ have been characterized by administration of exogenous peptide, but the function of endogenous apelin on the cardiovascular system is not well described. To investigate the tonic effects of apelin-APJ in contractile function, we generated and characterized apelin and APJ null mice. We also investigated the regulation of apelin and APJ receptor expression in response to cardiac pressure overload. While knockout apelin mice were viable and fertile, APJ null mice showed evidence of fetal wastage for approximately half of the embryos. Compared to wild-type controls, APJ deficient mice reaching adulthood had significantly reduced exercise capacity (treadmill runtime: 20.30 ± 0.974 vs. 23.64 ± 0.737, P=0.009) and LV fractional shortening by echocardiography (29.88 ± 0.927% vs. 44.93 ± 2.66%, P,0.0001). Isolated myocytes from APJ knockouts exhibited reduced sarcomeric shortening (5.070 ± ...

Heparin Binding EGF-like growth factor (HB-EGF), 50 µg. HB-EGF is an EGF related growth factor that signals through the EGF receptor, and stimulates the proliferation of smooth muscle cells (SMC), fibroblasts, epithelial cells, and keratinocytes.

HDGFRP2 - HDGFRP2 (untagged)-Human hepatoma-derived growth factor-related protein 2 (HDGFRP2), transcript variant 2 available for purchase from OriGene - Your Gene Company.

Heparin-binding EGF-like growth factor (HB-EGF) is synthesized as a type I transmembrane protein (proHB-EGF) and expressed on the cell surface. The ectodomain s

Murine Heparin-binding EGF-like Growth Factor stimulates smooth muscle cell regeneration and mitogenic activity. It may be implicated in atherosclerosis.

Cross-suppression of AREG/EREG expression may explain the tight co-expression of AREG and EREG, as well as their tendency to be more highly expressed than other EGFR ligands to determine Ctx efficacy. The positive selection for Ctx-resistant tumour cells exhibiting AREG/EREG cross-suppression may ha …

Sigma-Aldrich offers abstracts and full-text articles by [K Tatemoto, M Hosoya, Y Habata, R Fujii, T Kakegawa, M X Zou, Y Kawamata, S Fukusumi, S Hinuma, C Kitada, T Kurokawa, H Onda, M Fujino].

GAS7 antibody [9H6] (growth arrest-specific 7) for FACS, WB. Anti-GAS7 mAb (GTX84464) is tested in Human samples. 100% Ab-Assurance.

Shop Lens epithelium-derived growth factor ELISA Kit, Recombinant Protein and Lens epithelium-derived growth factor Antibody at MyBioSource. Custom ELISA Kit, Recombinant Protein and Antibody are available.

PC4 and SFRS1 interacting protein 1, also known as lens epithelium-derived growth factor (LEDGF/p75), dense fine speckles 70kD protein (DFS 70) or transcriptional coactivator p75/p52, is a protein that in humans is encoded by the PSIP1 gene. PSIP1 has not been clearly linked to a specific cellular mechanism. The term LEDGF/p75 (Lens epithelium-derived growth factor) has entered common usage based on the initial characterization of PSIP1, however this is a misnomer, as the protein is present in most tissues and has no direct role in the development of lens epithelium. LEDGF/p75, a transcription coactivator, gained prominence as a host factor that assists HIV integration and is probably the only integrase interactor whose knock-down severely affects the HIV integration levels. The interaction between HIV integrase and human LEDGF/p75 is a promising target for anti-HIV drug discovery. LEDGF/p75 recruits MLL complexes to HOX genes to regulate their expression. LEDGF/p52 is shown to recruit splicing ...

TY - JOUR. T1 - Effects of miR-335-5p in modulating osteogenic differentiation by specifically downregulating Wnt antagonist DKK1. AU - Zhang, Jin. AU - Tu, Qisheng. AU - Bonewald, Lynda F.. AU - He, Xi. AU - Stein, Gary. AU - Lian, Jane. AU - Chen, Jake. PY - 2011/8/1. Y1 - 2011/8/1. N2 - Dickkopf-related protein 1 (DKK1) is essential to maintain skeletal homeostasis as an inhibitor of Wnt signaling and osteogenic differentiation. The purpose of this study was to investigate the molecular mechanisms underlying the developmental stage-specific regulation of the DKK1 protein level. We performed a series of studies including luciferase reporter assays, micro-RNA microarray, site-specific mutations, and gain- and loss-of-function analyses. We found that the DKK1 protein level was regulated via DKK1 3 UTR by miRNA control, which was restricted to osteoblast-lineage cells. As a result of decreased DKK1 protein level by miR-335-5p, Wnt signaling was enhanced, as indicated by elevated GSK-3β ...

Granulin is a protein that in humans is encoded by the GRN gene. Granulins are a family of secreted, glycosylated peptides that are cleaved from a single precursor protein with 7.5 repeats of a highly conserved 12-cysteine granulin/epithelin motif. The 88 kDa precursor protein, progranulin, is also called proepithelin and prostate cancer (PC) cell-derived growth factor. Cleavage of the signal peptide produces mature granulin which can be further cleaved into a variety of active, 6 kDa peptides. These smaller cleavage products are named granulin A, granulin B, granulin C, etc. Epithelins 1 and 2 are synonymous with granulins A and B, respectively. Both the peptides and intact granulin protein regulate cell growth. However, different members of the granulin protein family may act as inhibitors, stimulators, or have dual actions on cell growth. Granulin family members are important in normal development, wound healing, and tumorigenesis. The human liver fluke (Opisthorchis viverrini) contributes to ...

Background The Wnt signalling pathway is an important regulator of adult tissue maintenance and homeostasis. Disorders in Wnt signaling cause human degenerative diseases as well as cancer. Dickkopf-1 (DKK-1) is negative regulator protein of the Wnt pathway and is associated with a variety of organic diseases. Under the hypothesis that DKK-1 as a regulator protein is involved in ventricular remodelling after myocardial infarction we aimed to investigate the prognostic value of serum measurement of DKK-1 in patients with acute coronary syndromes.. Methods and results From April 2003 until April 2005 1136 consecutive patients (age 64±12 years; 347 females) with an ACS within the last 48 hours, who were referred for early invasive diagnositc were included. Follow up data were available for 1128 (99.3%) patients. Serum samples on admission were available for 1092 (97%) patients and from 820 (72%) patients a second sample the day following admission. Values are expressed as median. Values of DKK-1 on ...

ViraQuest Inc. , Uncategorized , MiR-34a targeting of Notch ligand delta-like 1 impairs CD15+/CD133+ tumor-propagating cells and supports neural differentiation in medulloblastoma ...

The investigators are hypothesizing that decreasing estrogen levels will cause serum DKK1 to peak, then decrease gradually as estrogens reach a new lowe

The novel G protein-coupled receptor APJ, recently paired with the proposed cognate peptide ligand apelin, mediates potent vasodilator and positive inotropic actions in rats. Radioligand binding showed apelin receptors in rat and human heart and human large conduit vessels. The specific cell types e …

Primary cell cultures: human fetal material, oligodendrocyte isolation, enrichment, and maturation. Human fetal spinal cord tissue was obtained from the Einstein Human Fetal Tissue Repository (New York, NY) as approved by the Institutional Review Board. Tissues from abortuses of normal women were collected after elective pregnancy termination. Informed consent was obtained from all tissue donors. The ages of the abortuses were determined by multiple parameters, including the date of the last menstrual period by history, uterine size by bimanual and abdominal examination, ultrasonography by using predominantly the maximum biparietal diameter, and, postabortally, by measurement of fetal foot length (Streeter, 1920; Hern, 1984). After retrieval of tissue the spinal cords were stored in sterile medium with antibiotics on ice and were obtained from the neuropathologist within the hour. For cultures the oligodendrocytes were obtained from 21-23 gestational weeks (gw) human fetal spinal cords. The ...

Adipocytokines are polypeptides produced by fat cells.They are associated with obesity, hyperinsulinemia, chronic vascular disease and cancer.The adipocytokines which promote angiogenesis (the growth of new blood vessels) include vascular endothelial growth factor (VEGF,) hepatocyte growth factor (HGF,) leptin, tumour necrosis factor alpha, heparin-binding epidermal growth factor, insulin-like growth factor and interleukin-6 (IL-6.) Whole plant extracts […]. View Post ...

CRM197, an inhibitor of heparin-binding EGF-like growth factor (HB-EGF), produces a synergistic ovarian cancer anti-tumor effect when combined with paclitaxel, according to study results published in the March 15th issue of the International Journal of Cancer. The investigators, Dr. Shingo Miyamoto and his colleagues, are affiliated with the Fukuoka University in Japan. The treatment of…

Lrp5 ELISA Kit (Rat) (GWB-KBBAA4) | Quantitative Sandwich ELISA | Sample Types: serum, plasma and other biological fluids. | Species Reactivity: Mouse | Alias: Dickkopf-4, Dickkopf-related protein 4, Dkk-4, MGC25705

Apelin is an endogenous peptide capable of binding the apelin receptor (APJ), which was originally described as an orphan G-protein-coupled receptor. Apelin and APJ are widely expressed in various tissues and organ systems. They are implicated in different key physiological processes such as angiogenesis, cardiovascular functions, cell proliferation and energy metabolism regulation. On the other hand, this ligand receptor couple is also involved in several pathologies including diabetes, obesity, cardiovascular disease and cancer ...

Apelin is an endogenous peptide capable of binding the apelin receptor (APJ), which was originally described as an orphan G-protein-coupled receptor. Apelin and APJ are widely expressed in various tissues and organ systems. They are implicated in different key physiological processes such as angiogenesis, cardiovascular functions, cell proliferation and energy metabolism regulation. On the other hand, this ligand receptor couple is also involved in several pathologies including diabetes, obesity, cardiovascular disease and cancer ...

A population of monocytes, known as Ly6C(lo) monocytes, patrol blood vessels by crawling along the vascular endothelium. Here we show that endothelial cells control their origin through Notch signalling. Using combinations of conditional genetic deletion strategies and cell-fate tracking experiments we show that Notch2 regulates conversion of Ly6C(hi) monocytes into Ly6C(lo) monocytes in vivo and in vitro, thereby regulating monocyte cell fate under steady-state conditions. This process is controlled by Notch ligand delta-like 1 (Dll1) expressed by a population of endothelial cells that constitute distinct vascular niches in the bone marrow and spleen in vivo, while culture on recombinant DLL1 induces monocyte conversion in vitro. Thus, blood vessels regulate monocyte conversion, a form of committed myeloid cell fate regulation. ...

Apelin is an endogenous peptide capable of binding the apelin receptor (APJ), which was originally described as an orphan G-protein-coupled receptor. Apelin and APJ are widely expressed in various tissues and organ systems. They are implicated in different key physiological processes such as angiogenesis, cardiovascular functions, cell proliferation and energy metabolism regulation. On the other hand, this ligand receptor couple is also involved in several pathologies including diabetes, obesity, cardiovascular disease and cancer ...

Elabela/Toddler-32, Elabela/Toddler-21, Elabela/Toddler-11, [Pyr1]apelin-13, apelin-13, apelin-36, apelin-17, apelin receptor early endogenous ligand. ...

Betacellulin, Human, Recombinant Recombinant, human betacellulin consisting of amino acids 32-111 and expressed in E. coli. A heparin-binding protein that is able to bind to the EGF receptor. - Find MSDS or SDS, a COA, data sheets and more information.

Dickkopf-1 (DKK-1) is a secreted inhibitor of the Wnt signaling pathway. We previously identified DKK-1 as a candidate tumor suppressor and demonstrated that ectopic expression of the DKK-1 suppressed

Complete information for CECR2 gene (Protein Coding), CECR2 Histone Acetyl-Lysine Reader, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium

Mouse monoclonal antibody raised against a full length recombinant HBEGF. HBEGF (AAH33097.1, 20 a.a. ~ 208 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. (H00001839-M05) - Products - Abnova

CHO-Anti-Human HBEGF F(ab) stable cell line is clonally-derived from a CHO cell line, which has been transfected with an Anti-human HBEGF F(ab) gene to allow expression of the F(ab). It is an example of a cell line transfected using our proprietary CBTGS gene screening and amplification system.

Amphiregulin is an EGF related growth factor that signals through the EGF/TGF-a receptor, and stimulates growth of keratinocytes, epithelial cells and some fibroblasts.

The IUPHAR/BPS Guide to Pharmacology. amphiregulin ligand page. Quantitative data and detailed annnotation of the targets of licensed and experimental drugs.

Buy our Recombinant human Amphiregulin protein. Ab104355 is an active full length protein produced in Escherichia coli and has been validated in WB, FuncS…

AREG Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 98 amino acids and molecular mass of 11.3 KDa.

DKK-1 stands for Dickkopf-1 related protein. Human DKK-1 is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays. - Österreich

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SFRP1山羊多克隆抗体(ab110113)可与小鼠, 人样本反应并经IHC实验严格验证。中国75%以上现货，所有产品均提供质保服务，可通过电话、电邮或微信获得本地专属技术支持。