Cadherin has an intimate spatial relationship with actin filaments (AF) in various types of cells, forming the cell-to-cell adherens junction (AJ). We compared the AJ/AF relationship between non-polarized fibroblasts (NRK cells) and polarized epithelial cells (MTD-1A cells). E/P-cadherin, alpha-catenin, ZO-1 and vinculin were localized with reference to AF in these cells using laser scan microscopy as well as conventional light and electron microscopy. NRK cells adhered to each other at the tips of thin cellular processes, where spot-like AJ were formed, where P-cadherin, alpha-catenin, ZO-1 and vinculin were concentrated. Some stress-fiber-like AF bundles ran axially in these processes and terminated at spot-like AJ on their tips. At the electron microscopic level these spot-like AJ were seen as aggregates of small units of AJ, where AF were densely and perpendicularly associated with the plasma membrane. In MTD-1A cells, the AJ/AF relationship was investigated during the cell polarization ...
Myr 3, a member of the myosin-I family from rat, is shown in this study to be localized at adherens-type intercellular junctions in epithelial and nonepithelial tissues. Formation of intercellular junctions and the accompanying recruitment of myr 3 to these junctions involves signaling by the Rho subfamily of small GTP-binding proteins. This conclusion is based on studies with HtTA-1 HeLa cells that were induced by overexpression of constitutively active Cdc42Hs to form typical adherens-type intercellular junctions enriched in cadherins (N-cadherin), beta-catenin, filamentous actin and myr 3. Recruitement of myr 3 to Cdc42-induced adherens junctions in HeLa cells was dependent on a short region of the tail domain and a functional myosin motor domain, but was independent of its myosin-I tail homology and SH3 regions. Overexpression of constitutively active Rac1 induced a distinct type of adherens junction in HeLa cells that was characterized by elaborate intercellular interdigitations enriched in ...
TY - JOUR. T1 - Glycoproteins of coated pits, cell junctions, and the entire cell surface revealed by monoclonal antibodies and immunoelectron microscopy.. AU - Murphy, T. L.. AU - Decker, G.. AU - August, Thomas. PY - 1983/8. Y1 - 1983/8. N2 - Topographical descriptions of three major plasma membrane glycoproteins of murine 3T3 cells were obtained by immunoelectron microscopy with monoclonal antibodies. A glycoprotein of Mr 80,000 was distributed throughout the total cell surface. A second of Mr 90,000 was concentrated in coated pits, and a third of Mr 100,000 was localized at cell junctions.. AB - Topographical descriptions of three major plasma membrane glycoproteins of murine 3T3 cells were obtained by immunoelectron microscopy with monoclonal antibodies. A glycoprotein of Mr 80,000 was distributed throughout the total cell surface. A second of Mr 90,000 was concentrated in coated pits, and a third of Mr 100,000 was localized at cell junctions.. UR - ...
Endothelial leakiness or permeability directly determines the access of any bionanotechnology to the target tissue site. Currently, cancer nanotechnology relies on tumor-derived endothelial leakiness, which suffers from unreliability, inhomogeneity of leakiness, and uncontrollability. Nanomaterials by themselves are capable of inducing endothelial leakiness (NanoEL) without any tumor involvement by targeting the endothelial cell junctions; this NanoEL phenomenon not well understood. Here, we showed that the negatively charged Au nanoparticles (NPs) induce significantly higher NanoEL than positively charged nanoparticles. We hypothesized and showed that in both in vitro and in silico models that cell junction targeting arose for the negatively charged particles due to a succession of repulsive-sedimentary interactions between the negative particle and the negatively charged glycocalyx found on the cell membrane surface. On the contrary, NPs with positive charges are attracted stably by the ...
Sigma-Aldrich offers abstracts and full-text articles by [Masaya Akashi, Tomohito Higashi, Sayuri Masuda, Takahide Komori, Mikio Furuse].
Understanding the factors that regulate endothelial cell-cell junctions is important for many pathophysiological processes in which functional vascular integrity is compromised, such as development of neovasculature during angiogenesis and chronic inflammatory disorders. The present study shows that IQGAP1 colocalizes and forms a complex with VE-cadherin at the site of cell-cell contacts in unstimulated confluent HUVECs, and VEGF stimulation reduces their localization at the cell margin without affecting their complex formation. Knockdown of IQGAP1 using siRNA inhibits localization of VE-cadherin at cell-cell contacts as well as the following VEGF-stimulated events: (1) recruitment of VEGF2 to and the dissociation of α-catenin from the VE-cadherin/β-catenin complex; (2) ROS-dependent tyrosine phosphorylation of VE-cadherin, which is required for loss of cell-cell contacts8,9; and (3) capillary tube formation in 3-dimensional collagen gels. We also found that IQGAP1 expression is markedly ...
Rabbit polyclonal N Cadherin antibody - Intercellular Junction Marker. Validated in WB, IP, IHC, Flow Cyt, ICC/IF and tested in Mouse, Rat, Human, Pig, Xenopus laevis. Cited in 251 publication(s)…
Anti-N Cadherin antibody - Intercellular Junction Marker (ab18203) has been cited in 251 publications. References for Human, Mouse, Rat in ICC/IF, IF, IHC, IHC-Fr, IHC-P, IP, WB
Clone REA697 recognizes the rat CD146 (LSEC) antigen, also known as Gicerin, MCAM, MUC18, or MEL-CAM. CD146 is a putative cell adhesion molecule of an immunoglobulin (Ig) superfamily which shows homophilic and heterophilic binding activities with two isoforms: S-gicerin, which has small cytoplasmic domain and the same extracellular domain as l-gicerin, shows stronger cell adhesion activity. CD146 is expressed on endothelial cells and a variety of tumor cells and is involved in cell adhesion and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. In rat, neurite promotion activity of CD146 from hippocampal neurons is reported. Additional information: Clone REA697 displays negligible binding to Fc receptors. - Belgique
FHF3 span between extending and Be anxious image resolution (Fig. 3C). Measurements at 10s periods offered identical outcomes STF-62247 (Fig. H4G). Pretreatment with GdCl3 to stretch-loading got no impact prior, and dominated out advantages from stretch-activated Ca2+ stations (not really demonstrated). Cadherin inactivation with obstructing DECMA-1 antibody ablated the response (Fig. 3D). This instant, reversible switching suggests that -catenin features like an flexible springtime in series with the cytoskeleton, which deforms with the extracellular substrate and matrix. Assessment of the nanoprobe and MTC measurements shows that the Be anxious/ECFP reduce during bead rotating (Fig. 2B) can be credited to constant, mechanised perturbation than biochemical adaptation rather. Shape 3 Exogneous mechanised extend induce instant, reversible -catenin conformation switching at intercellular junctions Biochemical indicators STF-62247 during the 3s span between base extend and image resolution could ...
Plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development. Acts as surface receptor that triggers tyrosine phosphorylation of FYN and PTK2/FAK1, and a transient increase in the intracellular calcium concentration.
We described recently that DNAM-1 is involved in NK cell-mediated target cell killing of tumor cells by interacting specifically with PVR and Nectin-2 (2). Although PVR and Nectin-2 are expressed on tumor cell lines, they are also widely expressed on normal cells that are protected from NK cell-mediated lysis by their expression of HLA class I molecules. The aim of our analysis was to clarify the role of DNAM-1 interactions with its ligands in a normal setting. Nectins are homophilic and heterophilic cell adhesion molecules involved in several cell recognition processes, including the formation of cell junctions between epithelial cells and between neurons (10). For the first time, we demonstrate that PVR is the major ligand of DNAM-1 on endothelial cells. Our results highlight a role for DNAM-1 and PVR during leukocyte extravasation because they appear to be crucial for monocyte migration through endothelial junctions during the diapedesis step. This hypothesis is supported by the following ...
The work of Soda et al. (9) forces us to confront the role of endocytosis in the maintenance and regulation of the slit diaphragm and perhaps all junctional complexes. Significant advances in structural and basic cell biology have led to a clearer understanding of the structure and function of epithelial junctions, but what role could endocytosis be playing? The formation of adherent junction was thought to be made in two steps: delivery of cadherins to the surface resulted in a mobile pool, which then - apparently stabilized by cell-cell cadherin contacts - accumulated first into spots and then into long linear junctions. Advances in video microscopy have demonstrated that tight and adherent junctions are dynamic structures both in vivo and in the established junctions of confluent epithelial cell cultures. de Beco et al. studied "mature" epithelial junctions and found that most E-cadherin molecules did not diffuse in the plane of the membrane, but rather had a rapid turnover that was mediated ...
As for PKC, we found and identified ASIP, atypical PKC-specific interacting protein, as a physiological binding partner of atypical PKJKC isotypes. ASIP is a PDZ domain-containing adaptor-like protein and is the mammalian orthologue of C. elegans PAR-3 and Drosophila Bazooka, both of which are involved in the establishment of cell polarity. ASIP as well as aPKC localize to the tight junctions of vertebrate epithelial cells and are required for the establishment of epithelial cell polarith that include the development of epithelia-specific junctional structures ...
Intercellular junctions are structures that provide adhesion & communication between cells. They are mostly present in epithelial cells that are especially characterized by their strong attachment one to another and to the extracellular matrix ...
Figure 8. AdhRhoA2 induced changes in ZO-1 distribution in SC cells. Monolayers of 4-day confluent SC cells were infected with 500 pfu/cell AdhRhoA2. After 48 h, cells were fixed, permeablized and double-stained with specific antibodies against RhoA and ZO-1 using Cy2 (RhoA) and Cy3 (ZO-1) conjugated secondary antibodies. ZO-1 staining is present in the vehicle-treated cells at the cell-cell junctions and appear as a segmented border. Cells infected with AdhRhoA2 are devoid of this apportioned ZO-1 staining at the intercellular junctions. Original magnification noted at the right of the microphotographs.. ...
Blocking PVR-DNAM-1 transinteractions arrest monocytes over intercellular junctions at the apical surface of endothelial cells. Staining was performed after a
Research Interest: Ultrastructure and cell biology of the eye; structure-function relationship of intercellular junctions, cell membrane and cytoskeleton of the lens ...
Chapter 5 Histology• Study of Tissues• Epithelial Tissue• Connective Tissue• Nervous and Muscular Tissue• Intercellular Junctions, Glands an…
Karakesisoglou, I., Yang, Y. M. & Fuchs, E. (2000). An epidermal plakin that integrates actin and microtubule networks at cellular junctions. Journal Of Cell Biology 149(1): 195-208. ...
GO:0034331. The organization process that preserves a cell junction in a stable functional or structural state. A cell junction is a specialized region of connection between two cells or between a cell and the extracellular matrix. ...
ആവരണകലയിലെ കോശങ്ങളെ നന്നായി അടുക്കി ക്രമീകരിച്ചിരിക്കുന്നതിനാൽ കോശങ്ങൾക്കിടയിൽ കോശാന്ത്ര സ്ഥലം കുറവാണ്. അടുത്തടുത്ത രണ്ട് കോശങ്ങളുടെ പ്ലാസ്മാ സ്തരങ്ങൾ ചിലയിടങ്ങളിൽ കൂട്ടിയുറപ്പിച്ചിരിക്കുന്നു. ഇത്തരം സ്ഥാനങ്ങൾ കോശസന്ധികൾ (cell junctions). ടൈറ്റ് ജംങ്ഷനുകൾ ഉദാഹരണം. ഇത്തരം കോശസന്ധികൾ പദാർത്ഥസംവഹനത്തെ വൃതിവ്യാപനം (ഓസ്മോസിസ്), അന്തർവ്യാപനം (ഡിഫ്യൂഷൻ) എന്നിവ വഴി ...
This gene encodes a multi-domain scaffolding protein that is required for normal development. This protein may have a role in septate junction formation, signal transduction, cell proliferation, synaptogenesis and lymphocyte activation. Several alternatively spliced transcript variants encoding different isoforms have been described for this gene, but the full-length nature of some of the variants is not known. [provided by RefSeq, Feb 2011 ...
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Ill try to explain this best i can . We have 2 6509s running port channels down to 2 Nexus 7000 where the 7000s are running vpc . All the routing is still being done on the 6509s . The nexus 7000s only have routing running via
..., Are you so unhappy that you need to believe you will be happy after you die? In short, will that question get through a theists defenses? After my tw…
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So to answer the first question, yes I followed the stickied guide up top, and yes, it successfully flashed the bootloader, wiped all data, and flashed st…
An intercellular cleft is a channel between two cells through which molecules may travel and gap junctions and tight junctions may be present. Most notably, intercellular clefts are found between epithelial cells and the endothelium of blood vessels and lymphatic vessels, also helping to form the blood-nerve barrier surrounding nerves. Intercellular clefts are important for allowing the transportation of fluids and small solute matter through the endothelium. The dimensions of intercellular clefts vary throughout the body, however cleft lengths have been determined for a series of capillaries. The average cleft length for capillaries is about 20m/cm2. The depths of the intercellular clefts, measured from the luminal to the abluminal openings, vary among different types of capillaries, but the average is about 0.7 μm. The width of the intercellular clefts is about 20 nm outside the junctional region (i.e. in the larger part of the clefts). In intercellular clefts of capillaries, it has been ...
Here, we isolated a novel F-actin-binding protein with a molecular mass of ∼205 kD (p205). This protein was copurified with another protein with a molecular mass of 190 kD (p190) that lacked the F-actin-binding activity on various column chromatographies. The molecular cloning of the cDNAs of these two proteins revealed that the nucleotide sequence of the p190 cDNA was identical to that of the p205 cDNA, except for the two splicing regions. FISH analysis revealed that the genes of these two proteins were localized at the same locus. These results suggest that p205 and p190 are splicing variants derived from the same gene. Because a computer homology search revealed that the aa sequence of p190 was almost identical to that of human AF-6 protein, we theorize that p190 may be a rat counterpart of human AF-6 protein. We named p205 and p190 l- and s-afadins, respectively. Further purification steps of l-afadin, including Mono S column and Superdex 200 column chromatographies, did not separate l- ...
1959: Furshpan and Potter report that electrical stimulation that is insufficient to generate and action potential still allows current transfer between some nerve cells. [6] 1962: Dewey and Barr coin the term "nexus" to describe an intercellular connection between smooth muscle cells. [7] This term is now used interchangeably with the term "gap junction" 1963: Loewenstein and Kanno use microelectrodes and flurescent tracers to analyze the membrane permeability of epithelial cell junctions on Drosophilla salivary glands. The find that the junctional membrane surface is highly permeable so that small ions and fluorescent markers can move freely from one cell to the next.[8] 1963: Using thin slices of permanganate and osmium-fixed material, David Robinson discovers an array of hexagonal subunits in electrical synapses of Mauthner cells of goldfish. [9] 1963-1967: Difference between gap junction and tight junction remains unclear and confusing.[10] 1967: Karnovsky and Revel use a lanthanum salt ...
1959: Furshpan and Potter report that electrical stimulation that is insufficient to generate and action potential still allows current transfer between some nerve cells. [6] 1962: Dewey and Barr coin the term "nexus" to describe an intercellular connection between smooth muscle cells. [7] This term is now used interchangeably with the term "gap junction" 1963: Loewenstein and Kanno use microelectrodes and flurescent tracers to analyze the membrane permeability of epithelial cell junctions on Drosophilla salivary glands. The find that the junctional membrane surface is highly permeable so that small ions and fluorescent markers can move freely from one cell to the next.[8] 1963: Using thin slices of permanganate and osmium-fixed material, David Robinson discovers an array of hexagonal subunits in electrical synapses of Mauthner cells of goldfish. [9] 1963-1967: Difference between gap junction and tight junction remains unclear and confusing.[10] 1967: Karnovsky and Revel use a lanthanum salt ...
Looking for online definition of lateral surface of shaft of radius in the Medical Dictionary? lateral surface of shaft of radius explanation free. What is lateral surface of shaft of radius? Meaning of lateral surface of shaft of radius medical term. What does lateral surface of shaft of radius mean?
A-CAM (adherens-junction-specific cell adhesion molecule) is a calcium-dependent adhesion molecule which is associated with intercellular adherens junctions in various tissues (Volk & Geiger, 1986, J. Cell Biol. 103, 1441-1450 and 1451-1464). In the present report, we have investigated the distribution of A-CAM during avian morphogenesis by immunofluorescence microscopy and immunoblotting. A-CAM appeared at the onset of gastrulation on developing mesodermal and endodermal cells and was then expressed on tissues derived from the three primary germ layers. During embryonic life, A-CAM was constitutively expressed in a number of tissues including the central and peripheral nervous system, myocardium, muscles, notochord, skin and lens whereas it was found transiently in many tissues ranging from the nephritic tubules and the endoderm of visceral arches to ectodermal placodes. In the adult, in addition to the nervous system, A-CAM was restricted to the skin, lens, heart and testis, and exhibited an ...
ED01-03 Preclinical data have generated an interesting hypothesis that suggests that the sensitivity of NSCLC, pancreatic and colon cell lines and/or tumor xenografts to EGFR inhibitors is dependant upon the degree to which they have undergone an epithelial to mesenchymal transition (EMT). NSCLC lines which express the epithelial cell junction protein E-cadherin showed greater sensitivity to EGFR inhibition in vitro and in xenografts. In contrast, NSCLC lines having undergone EMT, expressing vimentin and/or fibronectin, were insensitive to the growth inhibitory effects of erlotinib. In the E-cadherin positive cells inhibition of EGFR activity leads to a reduction in the phosphorylation of Akt. In contrast, however, those cells that have lost E-cadherin expression and gained the expression of mesenchymal markers, do not show a reduction in phosphorylation of Akt even though EGFR is present and can be inhibited. Interestingly the E-cadherin expressing cells also express ErbB3 and we believe that ...
Plakins: A family of related proteins that associate with cytoskeletal elements and junctional complexes at INTERCELLULAR JUNCTIONS. Plakins share a common plakin domain or a plakin repeat domain.
Tumor blood vessels are leaky and immature, which causes inadequate blood supply to tumor tissues resulting in hypoxic microenvironment and promotes metastasis. Here we have explored tumor vessel modulating activity of Sac-1004, a recently developed molecule in our lab, which directly potentiates VE-cadherin-mediated endothelial cell junction. Sac-1004 could enhance vascular junction integrity in tumor vessels and thereby inhibit vascular leakage and enhance vascular perfusion. Improved perfusion enabled Sac-1004 to have synergistic anti-tumor effect on cisplatin-mediated apoptosis of tumor cells. Interestingly, characteristics of normalized blood vessels namely reduced hypoxia, improved pericyte coverage and decreased basement membrane thickness were readily observed in tumors treated with Sac-1004. Remarkably, Sac-1004 was also able to inhibit lung and lymph node metastasis in MMTV and B16BL6 tumor models. This was in correlation with a reduction in epithelial-to-mesenchymal transition of ...
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A low-resistance hydrocarbon-adsorptive cartridge for an air intake of an internal combustion engine comprising a structure for being mounted into a portion of an engine air intake system. The structure is adapted to orient and retain one or more thin sheets of activated carbon sheeting in the intake system. Preferably, a plurality of sheets is oriented such that the leading edge of each sheet is presented to the engine intake air stream, thereby minimizing reduction in total cross-sectional area of the intake system. Preferably, the one or more sheets are spaced apart by a distance that is small relative to the extent of the elements in the direction of engine air flow such that a high probability is created that hydrocarbons migrating out of a shut down engines intake manifold will encounter a surface of at least one of the adsorptive sheets and thus be adsorbed.
Epidermal junctions can be maintained in cell-adhesion mutants and RNAi treated animals.We used an AJM-1::GFP transgene (jcIs1) to examine epidermal morphology
Figure 3. The CeAJ and cell-cell adhesion. (A) Schematic representation of known components CeAJ components. Like in vertebrates and Drosophila, C. elegans epithelial cells contain two adhesion complexes, the cadherin-catenin (CCC) and the DLG-1/AJM-1 (DAC) complexes. C. elegans is unique in three respects: (i) there is a single electron-dense area in the CeAJ (see Figure 1B); (ii) LET-413 does not colocalize with DLG-1 (as its homologue Scribble in Drosophila); (iii) PAR-3, PAR-6, PKC-3 and CRB-1 are present at the apical membrane in tubular organs (the existence of apical polarity determinants in epidermal cells is an open question). CeAJs from different epithelia contain the same set of proteins; notable differences concern the identity of the classical claudin-like protein (CLC-1 present in the pharynx, vulva and spermatheca; CLC-2 present in the lateral epidermis). The DAC complex might correspond to the electron-density in the CeAJ. Indeed, immunogold staining experiments localize AJM-1 at ...
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In 1975, Orci et al. (1) reported that human islet cells contain specialized membrane domains that are compatible with the ultrastructural features of two types of intercellular junctions: tight junctions and gap junctions. Since then, numerous reports have demonstrated critical functions for these cell-cell junctional complexes in islet cells (2-8). Eventually, a number of proteins were identified that regulated cell aggregation, islet cell-type segregation, architectural organization within islets of Langerhans, and state of differentiation, cell growth, and hormone secretion (9-16). Hints that direct islet cell-to-islet cell interactions are required for proper insulin secretion were uncovered in the 1980s when it was observed that single (isolated) β-cells are unresponsive to glucose unless they are given the opportunity to reaggregate into small clusters (17). Even more interesting, it was observed that islet cell types harbor specific cell-to-cell recognition signatures that drive their ...
Cadherins, Ca2+-dependent adhesion molecules, are crucial for cell-cell junctions and remodeling. Cadherins form inter-junctional lattices by the formation of both cis and trans dimers. Here, we directly visualize and quantify the spatiotemporal dynamics of wild-type and dimer mutant N-cadherin interactions using time-lapse imaging of junction assembly, disassembly and a FRET reporter to assess Ca2+-dependent interactions. A trans dimer mutant (W2A) and a cis mutant (V81D/V174D) exhibited an increased Ca2+-sensitivity for the disassembly of trans dimers compared to the WT, while another mutant (R14E) was insensitive to Ca2+-chelation. Time-lapse imaging of junction assembly and disassembly, monitored in 2D and 3D (using cellular spheroids), revealed kinetic differences in the different mutants as well as different behaviors in the 2D and 3D environment. Taken together, these data provide new insights into the role that the cis and trans dimers play in the dynamic interactions of cadherins ...
View Notes - Exam 2 Qwizdom from BIO 172 at University of Michigan. Mutations in one of the junctional complexes below causes an irregular heartbeat. Which one do you think it is. A) B) C) D) E)
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The junction between neurons and their target cells at which an impulse is transmitted by either electrical or chemical means; the two cells are separated by a small gap called the synaptic...
I wish I had a copy of the images to show you, because theyre kind of amazing. Imagine a cross-section shot of a head and what isnt brain, is tumor. In the small gap at the base of the brain, there it is, and its huge. Tumor, all of it. So large you cant see…
I wish I had a copy of the images to show you, because theyre kind of amazing. Imagine a cross-section shot of a head and what isnt brain, is tumor. In the small gap at the base of the brain, there it is, and its huge. Tumor, all of it. So large you cant see…
Whenever we found an opening in the rock falls dad would use a candle to check the oxygen levels were OK and then hed squeeze me though a small gap to see what was on the other side. Some people might think it was an odd pastime for a kid, but I thought it was amazing ...