Borai, A, Livingstone, C, Mehta, S, Zarif, H, Abdelaal, F and Ferns, G (2009) Biological variation in fasting serum insulin-like growth factor binding protein-1 (IGFBP-1) among individuals with a varying glucose tolerance ...
We have investigated the expression and secretion of insulin-like growth factor binding proteins (IGFBPs-1 to -6) in human vascular smooth muscle cells (hVSMCs) cultured from human renal arteries. Solution hybridization was used to determine IGFBP nRNA levels and Western immunoblot to detect the corresponding peptides. The hVSMCs expressed mRNAs for IGFBPs-2 to -6, IGFBP-1 mRNA was not detected. IGFBPs-3, -4 and -6 mRNAs were the most abundant, IGFBP-5 was also highly expressed, whereas the IGFBP-2 mRNA was just above the limit of detection. Serum starvation for 48 h significantly decreased the mRNA levels of IGFBPs-2 to -5 and tended to decrease IGFBP-6 mRNA also. IGFBPs-2, -4, -5 and -6 peptides could be detected in conditioned medium, but IGFBP-3 peptide was not detected. IGFBP-4 was the only peptide detected without any concentration step. Low-molecular-mass immunoreactive degradation products were found for IGFBPs-2 and -4. Exogenous IGFBPs-1, -3 and -4 in concentrations of 50 ng/ml ...
TY - JOUR. T1 - Structural and immunological comparison of insulin-like growth factor binding proteins of cerebrospinal and amniotic fluids. AU - Rosenfeld, R. G.. AU - Pham, H.. AU - Conover, Cheryl A. AU - Hintz, R. L.. AU - Baxter, R. C.. PY - 1989. Y1 - 1989. N2 - The insulin-like growth factors (IGFs) found in plasma and a variety of other body fluids are complexed to specific binding proteins (BPs). The cDNA for a 25K IGF-BP was recently cloned and sequenced, and the primary structure of the BP deduced. This BP is found in amniotic fluid, decidual tissues, conditioned medium from HepG2 human hepatoma cells, and fetal plasma. An additional small IGF-BP has been identified in human cerebrospinal fluid (CSF). We now demonstrate that the IGF-BP found in CSF is structurally and immunologically distinct from that found in HepG2 conditioned medium. While the latter BP has approximately equal affinities for IGF-I, and -II, the CSF BP has a 10- to 20-fold greater affinity for IGF-II. In affinity ...
The present study establishes the cellular sites of expression of several components of the IGF system in adult rat liver, a predominant source of circulating IGF-I in adult rat and other mammals (17, 19). As expected, IGF-I mRNA was highly expressed in hepatocytes. IGF-I mRNAs were also demonstrated in Kupffer cells and hepatic endothelial cells. These findings indicate that IGF-I secreted from nonparenchymal cells may contribute to the circulating pool of IGF-I derived from liver, albeit at lower levels than IGF-I derived from hepatocytes. Kupffer and endothelial cells play a key role in immune recognition, cytokine production, and immune cell recruitment within the liver (11). In other systems, IGF-I expression by macrophages and endothelial cells may participate in processes such as wound healing (28) and vascular repair after endothelial damage (43) and in the pathogenesis of pulmonary and intestinal fibrosis (29, 51). Our present findings raise the possibility that IGF-I derived from ...
The views presented here are those of the author and are not to be construed as official or reflecting the views of the Uniformed Services University of the Health Sciences, the Department of Defense or the U.S. Government ...
Methods of treating a tumor in a subject include identifying a subject having, at risk for, or suspected of having a tumor, and administering to the subject an effective amount of an IGFBP7 agent if t
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Abstract The insulin-like growth factor (IGF) signaling pathway may be of importance for the proliferation of different tumours (e.g. breast cancer and Wilms tumour). The bioavailability of both IGF-I...
IGFBP3 - IGFBP3 (untagged)-Human insulin-like growth factor binding protein 3 (IGFBP3), transcript variant 1 available for purchase from OriGene - Your Gene Company.
IGFBP1 - IGFBP1 (untagged)-Human insulin-like growth factor binding protein 1 (IGFBP1) available for purchase from OriGene - Your Gene Company.
IGFBP3 (phospho Ser183) antibody (insulin-like growth factor binding protein 3) for WB. Anti-IGFBP3 (phospho Ser183) pAb (GTX55408) is tested in Human, Rat samples. 100% Ab-Assurance.
IGFBP7 antibody [3N37] (insulin-like growth factor binding protein 7) for WB. Anti-IGFBP7 mAb (GTX52799) is tested in Human samples. 100% Ab-Assurance.
1657 The effects of insulin-like growth factor binding protein-5 (IGFBP-5) on pancreatic cancer (PaC) cell lines transfected with vector (/Vec) or expressing a low (/BP5L) or high (/BP5H) level of IGFBP-5 showed that IGFBP-5 can inhibit (PANC-1) or enhance (MIA PaCa-2 and BxPC-3) cell growth. Cell cycle analysis revealed that in PANC-1 cells, IGFBP-5 induced G2/M cell cycle arrest independent of growth conditions. In MIA PaCa-2 cells, IGFBP-5 expression resulted in fewer cells in S phase compared to control cells (with or without serum) and an increase of cells in G2/M under normal growth conditions; whereas an increase of cells in G0/G1 was observed under serum-free conditions. In BxPC-3 cells grown with serum IGFBP-5 expression produced fewer cells in G0/G1 and an increase of cells in S phase. When grown without serum these effects were enhanced and lead to an increase of cells in G2/M. The PI3K and MAPK pathways were examined by western analysis to detect the activation status of Akt or ...
The protein encoded by this gene is a member of the DOK family of membrane proteins, which are adapter proteins involved in signal transduction. The encoded protein interacts with phosphorylated receptor tyrosine kinases to mediate neurite outgrowth and activation of the MAP kinase pathway. Unlike other DOK family proteins, this protein does not interact with RASGAP. This protein is up-regulated in patients with systemic sclerosis and is associated with fibrosis induced by insulin-like growth factor binding protein 5. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jun 2014 ...
Title of Dissertation: FUNCTION OF INSULIN-LIKE GROWTH FACTOR BINDING PROTEIN 7(IGFBP7) IN HEPATOCELLULAR CARCINOMA By Dong Chen. Purpose: Hepatocellular carcinoma (HCC) is a highly virulent malignancy with no effective treatment, thus requiring the development of innovative and effective targeted therapies. The oncogene Astrocyte Elevated Gene-1 (AEG-1) plays a seminal role in hepatocarcinogenesis and profoundly downregulates Insulin-like Growth Factor Binding Protein-7 (IGFBP7). The present study focuses on analyzing potential tumor suppressor functions of IGFBP7 in HCC and the relevance of IGFBP7 downregulation in mediating AEG-1 function. Experimental Design: IGFBP7 expression was detected by immunohistochemistry in HCC tissue microarrays by real-time PCR and ELISA in human HCC cell lines. Dual Fluorescence in situ hybridization was performed to detect loss of heterozygosity at the IGFBP7 locus. Stable IGFBP7- overexpressing clones were established in the background of AEG-1- overexpressing human
Expression of insulin-like growth factor binding protein 5 (IGFBP5) is strongly induced upon activation of hepatic stellate cells and their transdifferentiation into myofibroblasts in vitro. This was confirmed in vivo in an animal model of liver fibrosis. Since IGFBP5 has been shown to promote fibrosis in other tissues, the aim of this study was to investigate its role in the progression of liver fibrosis. The effect of IGFBP5 was studied in LX2 cells, a model for partially activated hepatic stellate cells, and in human primary liver myofibroblasts. IGFBP5 signalling was modulated by the addition of recombinant protein, by lentiviral overexpression, and by siRNA mediated silencing. Furthermore, the addition of IGF1 and silencing of the IGF1R was used to investigate the role of the IGF-axis in IGFBP5 mediated effects. IGFBP5 enhanced the survival of LX2 cells and myofibroblasts via a |50% suppression of apoptosis. This effect of IGFBP5 was not modulated by the addition of IGF1, nor by silencing of the
Insulin-like growth factor binding protein-6 (IGFBP-6) is a growth inhibitory protein that regulates the availability of insulin-like growth factors (IGFs). We recently reported that IGFBP-6 exerts intracellular actions via its translocation to the nucleus. We now show that IGFBP-6 co-purifies by tandem-affinity with nuclear proteins involved in DNA stability and repair such as Ku80, Ku70, histone H2B and importin-alpha. Furthermore, this report shows that IGFBP-6 and Ku80 interact specifically using two active binding sites for Ku80 in IGFBP-6. One of the binding sites [196RKR199], as part of the NLS-sequence in IGFBP-6 also binds importin-alpha which may selectively compete with Ku80 regulating its trafficking to the nucleus. Moreover, IGFBP-6 co-localized with Ku80 based on a cell cycle pattern. Overexpression of IGFBP-6 increased the nuclear Ku80 in mitotic cells and reduced it post-mitosis. It is known that if highly expressed IGFBP-6 induces apoptosis and in our model, the down-regulation of Ku80
TY - JOUR. T1 - Biochemical analysis of prostate specific antigen-proteolyzed insulin-like growth factor binding protein-3. AU - Fielder, P. J.. AU - Rosenfeld, R. G.. AU - Graves, H. C.B.. AU - Grandbois, K.. AU - Maack, C. A.. AU - Sawamura, S.. AU - Ogawa, Y.. AU - Sommer, A.. AU - Cohen, P.. PY - 1994/12/1. Y1 - 1994/12/1. N2 - Prostate specific antigen (PSA) has been shown to proteolyze. IGFBP-3. However, the cleavage sites and mechanism of proteolysis are unknown. In this study, we proteolyzed recombinant human IGFBP-3 with PSA bound to a solid phase support. The reaction mixture was separated by centrifugation, with PSA remaining in the solid phase and the proteolyzed IGFBP-3 in the aqueous phase, The IGPBP-3 fragments were functionally analyzed by affinity labeling and Western ligand blotting (WLB). Further biochemical analyses were provided by silver staining of total protein and Western immunoblotting (WIB) of immunoreactive fragments with an IGFBP-3 specific antiserum (α-BP-3 gl). ...
We have previously demonstrated that insulin-like growth factor binding protein-5 (IGFBP-5) is upregulated following treatment of the mouse mammary epithelial cell line HC11 with lactogenic hormones (dexamethasone, insulin, and prolactin-DIP). In addition, we have also shown that IGFBP-5 is upregulated in mammary epithelial cells in vivo during involution of the rodent mammary gland. We have, therefore, postulated that there may be a dual regulation of IGFBP-5 expression during the temporally separated processes of differentiation and apoptosis of mammary epithelial cells. To test this hypothesis further, we have used a phenotypically differentiated model, which comprises primary cultures of mouse mammary epithelial cells grown on a layer of EHS (Engelbreth-Holm-Swarm) extracellular matrix. We show that lactogenic hormone treatment hydrocortisone, insulin, and prolactin-HIP) of these cultures induces the upregulation of IGFBP-5 thus replicating the results obtained with the HC11 cell line. In ...
AIM: To investigate the role of insulin-like growth factor binding protein-7 (IGFBP-7) in the activation and transdifferentiation of hepatic stellate cells (HSC) in vitro. METHODS: Rat HSC-T6 cells were cultured in separate dishes and treated with various concentration of transforming growth factor (TGF)-β1, IGFBP-7 or anti-IGFBP-7 antibody for 24 h. The supernatant or a cytoplasm suspension was obtained from cultured HSC, followed by transfer of cells to form cell-coated dishes. Immunocytochemistry and Western blotting were used to analyze the expression of IGFBP-7 induced by TGF-β1 and the level of fibronectin, collagen I and (α-smooth muscle actin (SMA). The pro-apoptotic effect of anti-IGFBP-7 antibody was determined by flow cytometry. RESULTS: Immunocytochemistry and Western blotting revealed that the expression of IGFBP-7 in TGF-β1 treated HSC was significantly up-regulated compared to that in the control group. In addition, fibronectin, collagen I and α-SMA also showed ...
Vascular function is greatly influenced by growth factors and regulatory molecules that can interact with each other in a complex pattern in the vascular wall. In this thesis we studied how different substances of special interest in the pathogenesis of vascular disease interact and regulate each others expressions in endothelial cells and vascular smooth muscle cells (VSMCs).. In VSMCs, angiotensin II was shown to delay PDGF-BB induced cell growth. This transient inhibitory effect of angiotensin II was mediated by the AT1-receptor, did not involve autocrine action of transforming growth factor-ß1 (TGF-ß1) and acted at a site downstream of PDGF-ß receptor phosphorylation.. The interaction of the insulin-like growth factor-system (IGF-system) with various growth factors, glucose and nitric oxide (NO) was studied in vascular cells. Vascular endothelial growth factor (VEGF) and transforming growth factor-ß1 (TGF-ß1) regulated the expression of insulin-like growth factor-binding proteins ...
Insulin-like growth factor-binding protein-2 and -3 in gingival crevicular fluid.: These results indicate that IGFBP-2 is a potential novel marker for periodont
Introduction: In this study, we compared human placental gene expression patterns of IGF from pregnancies that ended with preterm delivery versus full term pregnancies as controls. We also assessed differences in clinical characteristics in the two groups. Materials and Methods: We used real-time PCR to assess gene expression patterns of IGF in human placental samples from 104 preterm and 140 full term pregnancies (control group) at the time of delivery. Clinical data were collected from our computerized database. Results: Pre-gestational Body Mass Index (BMI) was not significantly different in the two groups. In the preterm delivery group, the proportion of smokers was 26.9%, significantly higher than in the control group (7.1%, p,0.05). Preterm delivery began with premature rupture of membranes in 70.2% and spontaneous uterine activity in 29.8%. History of preterm delivery was present in 14.4% in the preterm delivery group compared to only 4.3% of the control group (p,0.05). In preterm ...
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European Cells & Materials Journal - Open and Free Access - The Official Research Journal of AOCMF, AOTrauma, European Orthopaedic Research Society (EORS), Swiss Society for Biomaterials (SSB) and Tissue & Cell Engineering Society (TCES)
Background: Immunization against self-antigens can induce regulatory responses that inhibit the development of desirable Type I antitumor immune responses. Removing epitopes that bias toward a regulatory phenotype may enhance vaccine efficacy. We developed a novel IGFBP-2 targeting DNA plasmid vaccine capable of selectively inducing Type I immunity. IGFBP-2 is an important regulator of ovarian cancer invasiveness and metastases. Eradication of cancer cells expressing IGFBP-2 through effective immunization could prevent disease relapse or metastatic spread. Methods: In a single-arm non-randomized study of advanced stage (III/IV) or recurrent ovarian cancer patients treated to complete remission after primary or salvage therapy, 25 patients received 3 monthly doses of an IGFBP-2 DNA vaccine by intradermal injection. All adverse events (AE) were reported using the Common Terminology Criteria for Adverse Events Version 4.0. Overall survival (OS) was analyzed using the Kaplan-Meier method. ELISPOT ...
Plasmid constructions. The IGFBP-3 sequences were excised from pSF202, pSF210, pSF211, and pSF207 containing the sequences for IGFBP-3 wild type, IGFBP-3KED253-255RGD, IGFBP-3 228KGRKR232NLS228MDGEA232, and IGFBP-3 Δ185-264, respectively ( 20) by NheI/HindIII and inserted into the cytomegalovirus (CMV) promoter-driven expression vector pX using SpeI/HindIII to generate pXIGFBP-3, pXIGFBP-3KED253-255RGD, and pXIGFBP-3 228KGRKR232NLS228MDGEA232. pXΔls-IGFBP-3 plasmids series: (a) To generate a new ATG start-site for the IGFBP-3 open reading frames without signal peptide (Δls), the oligonucleotide 5′-AATTCCATATGGGCGCGAGCTCGATATCA-3′ (MWG Biotech, Ebersberg, Germany) was inserted into the plasmid pUC19 digested with EcoRI and HindIII. The resulting plasmid is referred to pUC19-ATGnew. (b.1) To generate pXΔls-IGFBP-3, pXΔls-IGFBP-3KED253-255RGD, and pXΔls-IGFBP-3228KGRKR232NLS228MDGEA232, the plasmids pSF202, pSF210, and pSF211 were digested with HindIII and partially digested with SacI, ...
Aim:To correlate placental protein levels of insulin-like growth factor (IGF)-I and insulin-like growth factor binding protein (IGFBP)-1, with previously determined levels of IGF-I and IGF-II mRNA expression, and the micronutrients zinc and iron, and maternal and newborn anthropometry. Methods: Placental samples were collected from rural field sites in Pakistan. Samples were divided into small and large for gestational age groups (SGA and LGA, respectively). IGFBP-1 levels were assessed using Western immunoblotting. IGF-I protein levels were assessed using ELISA techniques. IGF mRNA expression, zinc, and iron, were quantified as previously described and were used for comparative Purposes only. Results: Thirty-three subjects were included (SGA, n = 12, LGA n = 21). Higher levels of IGFBP-1 were seen in the SGA group (p | 0.01). IGFBP-1 correlated positively with maternal and infant triceps skin-fold thickness in the LGA and SGA groups, respectively (p | 0.05). Significantly lower IGF-I protein levels
This gene is a member of the insulin-like growth factor binding protein (IGFBP) family and encodes a protein with an IGFBP domain and a thyroglobulin type-I domain. The protein binds both insulin-like growth factors (IGFs) I and II and circulates in the plasma. Binding of this protein prolongs the half-life of the IGFs and alters their interaction with cell surface receptors. [provided by RefSeq, Jul 2008 ...
Sigma-Aldrich offers abstracts and full-text articles by [Qiang You, Yan Wu, Nannan Yao, Guannan Shen, Ying Zhang, Liangguo Xu, Guiying Li, Cynthia Ju].
Litter size is among the most important traits in swine breeding. However, information on the genetics of litter size in pigs is lacking. In this study, we identified single nucleotide polymorphisms (SNPs) in the insulin-like growth factor binding protein 2 and 3 (IGFBP2 and IGFBP3) genes in Berkshire pigs and analyzed their association with litter size traits. The IGFBP2 SNP was located on chromosome 15 intron 2 (455, A , T) and the IGFBP3 SNP was on chromosome 18 intron 2 (53, A , G). The AT type of IGFBP2 and the GG type of IGFBP3 had the highest values for all litter size traits including total number born (TNB), number of pigs born alive, and breeding value according to TNB ...
PAPPA Full-Length MS Protein Standard (NP_002572), Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine, was produced in human 293 cells (HEK293) with fully chemically defined cell culture medium to obtain incorporation efficiency at Creative-Proteomics. This gene encodes a secreted metalloproteinase which cleaves insulin-like growth factor binding proteins (IGFBPs). It is thought to be involved in local proliferative processes such as wound healing and bone remodeling. Low plasma level of this protein has been suggested as a biochemical marker for pregnancies with aneuploid fetuses.
J:58580 van Kleffens M, Groffen CA, Dits NF, Lindenbergh-Kortleve DJ, Schuller AG, Bradshaw SL, Pintar JE, Zwarthoff EC, Drop SL, van Neck JW, Generation of antisera to mouse insulin-like growth factor binding proteins (IGFBP)-1 to -6: comparison of IGFBP protein and messenger ribonucleic acid localization in the mouse embryo. Endocrinology. 1999 Dec;140(12):5944-52 ...
J:58580 van Kleffens M, Groffen CA, Dits NF, Lindenbergh-Kortleve DJ, Schuller AG, Bradshaw SL, Pintar JE, Zwarthoff EC, Drop SL, van Neck JW, Generation of antisera to mouse insulin-like growth factor binding proteins (IGFBP)-1 to -6: comparison of IGFBP protein and messenger ribonucleic acid localization in the mouse embryo. Endocrinology. 1999 Dec;140(12):5944-52 ...
Kirman I, Cekic V, Poltaratskaia N, et al. Plasma from patients undergoing major open surgery stimulates in vitro tumor growth: lower insulin-like growth factor binding protein 3 levels may, in part, account for this change. Surgery 2002;132:186-92.PubMedCrossRefGoogle Scholar ...
TY - JOUR AU - Resanović, Ivana AU - Gluvić, Zoran AU - Zarić, Božidarka AU - Sudar-Milovanović, Emina AU - Vučić, Vesna AU - Arsić, Aleksandra AU - Nedić, Olgica AU - Šunderić, Miloš AU - Gligorijević, Nikola AU - Milačić, Davorka AU - Isenović, Esma R. PY - 2020 UR - http://vinar.vin.bg.ac.rs/handle/123456789/8567 T2 - Canadian Journal of Diabetes T1 - Effect of Hyperbaric Oxygen Therapy on Fatty Acid Composition and Insulin-like Growth Factor Binding Protein 1 in Adult Type 1 Diabetes Mellitus Patients: A Pilot Study VL - 44 IS - 1 SP - 22 EP - 29 DO - 10.1016/j.jcjd.2019.04.018 ER ...
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Insulin-like growth factor binding protein-6 (IGFBP-6), the main regulator of insulin-like growth factor-2 (IGF-2), is a component of the stem cell niche in developing muscle cells. However, its role in muscle development has not been clearly defined. In this study, we investigated the role of IGFBP-6 in muscle commitment and differentiation of human mesenchymal stem cells derived from the placenta. We showed that placental mesenchymal stem cells (PMSCs) have the ability to differentiate into muscle cells when exposed to a specific culture medium by expressing muscle markers Pax3/7, MyoD, myogenin, and myosin heavy chain in a stage-dependent manner with the ultimate formation of multinucleated fibers and losing pluripotency-associated markers, OCT4 and SOX2 ...
Low serum levels of insulin-like growth factor-1 (IGF-1) and insulin-like growth factor binding protein-3 (IGFBP-3) are associated with Alzheimers Disease in men, but not women, according to a recent study accepted for publication ...
TY - JOUR. T1 - Not all insulin-like growth factor-binding proteins (IGFBPs) are detectable by western ligand blotting. T2 - Case studies of pc 12 pheochromocytoma and rat anterior pituitary igfbps and proteolyzed igfbp-3. AU - Ocrant, Ian. AU - Fay, Charles T.. AU - Pham, Hung. AU - Rosenfeld, Ron G.. PY - 1992/7. Y1 - 1992/7. N2 - We studied the limitations of the Western ligand blot (WLB) for detecting insulin-like growth factor-binding proteins (IGFBPs). PC12 rat pheochromocytoma cells and rat anterior pituitary cells (AP) secrete IGFBPs that cannot be detected by WLB. We used affinity labeling, WLB, dot blotting, competitive binding, ion exchange chromatography, and deglycosylation to characterize these IGFBPs. These IGFBPs were compared with pregnancy protease-derived IGFBP-3 fragments that also bind insulin-like growth factors (IGFs), but are not detectable by WLB. We showed that PC12 IGFBP is cationic, not glycosylated, with 25, 500 mol wt reduced (18, 500 unreduced), with high affinity ...
Insulin-like growth factor-binding protein 6 contains a PF00086 domain.. Insulin-like growth factor-binding protein 6 contains a PF00219 domain.. Insulin-like growth factor-binding protein 6 is proteolytically cut by matrix metallopeptidase-2 (M10.003) cleavage. CLRR-EGQP.. ...
The insulin-like growth factors (IGFs), their receptors, and their binding proteins play key roles in regulating cell proliferation and apoptosis. Insulin-like growth factor binding protein-3 (IGFBP3, OMIM #146732) is one of the proteins that bind to the IGFs. IGFBP3 is a modulator of IGF bioactivity, and direct growth inhibitor in the extravascular tissue compartment. We identified twenty-two novel single nucleotide polymorphisms (SNPs) in IGFBP3 gene in Korean cattle (Hanwoo, Bos taurus coreanae) by direct sequencing of full gene including -1,500 bp promoter region. Among the identified SNPs, five common SNPs were screened in 650 Korean cattle; one SNP in promoter (IGFBP3 G-854C), one in 5`UTR region (IGFBP3 G-100A), two in intron 1 (IGFBP3 G+421T, IGFBP3 T+1636A), and one in intron 2 (IGFBP3 C+3863A). The frequencies of each SNP were 0.357 (IGFBP3 G-854C), 0.472 (IGFBP3 G-100A), 0.418 (IGFBP3 G+421T), 0.363 (IGFBP3 T+1636A) and 0.226 (IGFBP3 C+3863A), respectively. Haplotypes and their ...
Prostate Cancer Risk in Relation to a Single Nucleotide Polymorphism in the Insulin-like Growth Factor-binding Protein-3 (IGFBP3) Gene: a Meta-analysis IGFBP3;prostate cancer;polymorphism;meta-analysis; Insulin-like growth factor-binding protein-3 (IGFBP3) has been identified as a putative tumor suppressor with multifunctional roles in the IGF axis. Recently, there have been a growing body of studies investigating the relation between the IGFBP3 A-202C polymorphism, circulating IGFBP3 and prostate cancer risk, but their outcomes varied leading to controversy. Hence, it is necessary to perform a meta-analysis covering all eligible studies to shed a light on the association of IGFBP3 A-202C and cancer risk. Finally, we included a total of 11 relevant articles between 2003 and 2010 covering 14 case-control studies including 9,238 cases and 8,741 controls for our analysis. Our results showed that A-202C was a marginal risk factor of prostate cancer (allele contrast: OR=1.08, 95% CI :1.01-1.16; dominant
The primary aim of this text is to gain insight on how cellular activation by a insulin-like growth factor (IGF-I), in the presence of insulin-like growth factor binding protein-3 (IGFBP-3), is influenced by heparan sulfate proteoglycans (HSPG). Initial research will be presented, assumptions and hypotheses that were included in the development of mathematical models will be discussed, and the future enhancements of the models will be explored. There are many potential scenarios for how each component might influence the others. Mathematical modeling techniques will highlight the contributions made by numerous extracellular parameters on IGF-I cell surface binding. Tentative assumptions can be applied to modeling techniques and predictions may aid in the direction of future experiments. Experimentally, it was found that IGFBP-3 inhibited IGF-I Bovine Aortic Endothelial (BAE) cell surface binding while p9 HS slightly increased IGF-I BAE cell surface binding. IGFBP-3 has a higher binding affinity ...
Insulin-like growth factor binding proteins (IGFBPs) are known to be important modulators of the insulin-like growth factor (IGF-I). However, their precise role is as yet unclear. Further, recent studies have indicated that IGFBP-3 has a receptor mediated growth inhibitory response of its own. In the present study, we quantified the binding characteristics of IGFBP-3 to bovine aortic endothelial (BAE) cells. Binding studies at 4 oC were conducted and a specific binding curve for IGFBP-3 was obtained. IGFBP-3 was found to bind with an equilibrium dissociation constant (KD) value of 3.1 x 10-10 M. The role of heparan sulfate proteoglycans (HSPG) in the IGFBP-3 binding mechanism was also examined. It was seen that inactivation of the cell surface HSPGs with 75 mM sodium chlorate did not affect IGFBP-3 binding. Further, there have been reports of inhibition of IGFBP-3 binding by heparin in the media. Hence, the most probable interaction of HSPG with IGFBP-3 occurs in the extracellular region, with ...
Introduction: Heart failure with preserved ejection fraction (HFpEF) is associated with considerable morbidity and mortality. Insulin-like growth factor-binding protein 7 (IGFBP7) is a cell cycle arrest biomarker associated with abnormal diastology and prognosis in HF with reduced EF (HFrEF). Its role in patients with HFpEF is unknown.. Hypothesis: IGFBP7 will be associated with adverse outcomes in HFpEF.. Methods: Baseline (BL) IGFBP7 (n=302; placebo=154, irbesartan=148) and 6 month change (Δ; n=293; placebo=147, irbesartan=146) were measured and correlated with clinical data, biomarkers, estimated glomerular filtration rate (eGFR), and the primary outcome of all-cause mortality (ACM) or cardiovascular hospitalization (CVH) in patients from the Irbesartan in HFpEF (I-PRESERVE) Trial; secondary outcomes included HF events.. Results: Median BL IGFBP7 concentration was 218 ng/mL, higher than previously seen in patients with HFrEF. BL IGFBP7 was correlated with age (R2=0.13; P,0.0001) and other ...
Fisch, Thomas Martin (2005): Effects of insulin-like growth factor-binding protein-2 (IGFBP-2) overexpression on adrenal and renal growth processes and functions: findings in transgenic mouse models. Dissertation, LMU München: Tierärztliche Fakultät ...