Hepatic ischaemia/reperfusion (I/R) injury is a major cause of primary non-function of the graft after liver transplantation. The ability to assess the severity of ischaemic injury would be of prognostic value and allow the possibility of therapeutic interventions. Currently there is no reliable clinical method for assessing the severity of hepatic ischaemic injury. The hepatic handling of Indocyanine Green as a technique for monitoring the severity of I/R injury has been investigated in the present study. A rabbit model of lobar ischaemia was used. At laparotomy, left lobe hepatic ischaemia was produced for 30, 45 or 60min, followed by 60min of reperfusion. Liver function tests, bile excretion and flow in the hepatic microcirculation were measured in animals subjected to I/R injury and in controls. Indocyanine Green was given after reperfusion and its concentration was measured directly in the liver using near-infrared spectroscopy. Indocyanine Green hepatic uptake and excretion rates were ...
TY - JOUR. T1 - Intravenous indocyanine green dye is insufficient for robust immune cell labelling in the human retina. AU - Bell, Oliver H. AU - Carreño, Ester. AU - Williams, Emily L. AU - Wu, Jiahui. AU - Copland, David A. AU - Bora, Monalisa. AU - Kobayter, Lina. AU - Fruttiger, Marcus. AU - Sim, Dawn A. AU - Lee, Richard W J. AU - Dick, Andrew David. AU - Chu, Colin J. PY - 2020/2/13. Y1 - 2020/2/13. N2 - It is not currently possible to reliably visualise and track immune cells in the human central nervous system or eye. Previous work demonstrated that indocyanine green (ICG) dye could label immune cells and be imaged after a delay during disease in the mouse retina. We report a pilot study investigating if ICG can similarly label immune cells within the human retina. Twelve adult participants receiving ICG angiography as part of routine standard of care were recruited. Baseline retinal images were obtained prior to ICG administration then repeated over a period ranging from 2 hours to 9 ...
TY - JOUR. T1 - Feasibility of indocyanine green fluorescence imaging for intraoperative identification of parathyroid glands during thyroid surgery. AU - van den Bos, Jacqueline. AU - van Kooten, Lottie. AU - Engelen, Sanne M. E.. AU - Lubbers, Tim. AU - Stassen, Laurents P. S.. AU - Bouvy, Nicole D.. PY - 2019/2. Y1 - 2019/2. KW - indocyanine green. KW - near-infrared fluorescence imaging. KW - NIRF. KW - parathyroid gland. KW - thyroidectomy. KW - METHYLENE-BLUE. KW - COMPLICATIONS. KW - ANGIOGRAPHY. KW - PERFUSION. U2 - 10.1002/hed.25451. DO - 10.1002/hed.25451. M3 - Article. VL - 41. SP - 340. EP - 348. JO - Head and Neck-Journal for the Sciences and Specialties of the Head and Neck. JF - Head and Neck-Journal for the Sciences and Specialties of the Head and Neck. SN - 1043-3074. IS - 2. ER - ...
Leptospirosis is one of the leading global zoonotic causes of morbidity and mortality. It is induced by a pathogenic spirochete of the genus Leptospira. The icteric form of leptospirosis is characterized by pronounced hyperbilirubinemia and associated with significantly increased mortality. Conventional static liver function tests insufficiently assess hepatic damage and have limited prognostic value. Dynamic tests, such as indocyanine green plasma (ICG) clearance, more adequately reflect hepatic functional status. In this case report we describe the ICG plasma disappearance rates (ICG-PDR) in a patient with leptospirosis and massive hyperbilirubinemia, expanding our knowledge of liver dysfunction in icteric leptospirosis. A 21-year-old Caucasian man presented with acute-onset jaundice, myalgia, fever and headaches. Laboratory tests upon admission revealed, most notably, acute kidney failure and hyperbilirubinemia of 17 mg/dl with mild elevation of aminotransferases. In the course of the following 4
In contrast to indocyanine green (ICG) blood clearance, the plasma disappearance rate (PDR) of ICG does not require absolute ICG blood concentrations and today can be assessed transcutaneously. In thi
After approval by our ethics committee and written consent, we postoperatively studied 12 patients (mean age 71 ± 8 years) with elective coronary artery bypass grafting (n = 2) or aortic valve replacement (n = 10). All patients had a reduced left ventricular function and underwent extended hemodynamic monitoring by a pulmonary artery (CCO-PAC) for clinical indication. Microcirculation within the splanchnic area was assessed by gastric tonometry, liver blood flow and function non-invasively by transcutaneous measurement of the ICG-PDR. Since fluid loading led to no increase in cardiac output, patients were considered nonfluid responsive. Measurements were made on ICU admission and after 1 hour of epinephrine treatment. The mean epinephrine dosage was changed from 0.02 to 0.08 μg/kg/min. All patients were on pressure-controlled mechanical ventilation and respirator settings remained unchanged throughout the study period. Data are the mean ± SD. P , 0.05 was considered statistically significant. ...
To quantitate the dilution curves, standard dilutions of Indocyanine Green in whole blood are made as follows: It is strongly recommended that the same dye that was used for the injections be used in the preparation of these standard dilutions. The most concentrated dye solution is made by accurately diluting 1 mL of the 5-mg/mL dye with 7 mL of distilled water. This concentration is then successively halved by diluting 4 mL of the previous concentration with 4 mL of distilled water. (If a 2.5 mg/mL concentration was used for the dilution curves, 1 mL of the 2.5 mg/mL dye is added to 3 mL of distilled water lo make the most concentrated "standard solution. This concentration is then successively halved by diluting 2. mL of the previous concentration with 2 mL of distilled water.) Then 0.2-mL portions (accurately measured from a calibrated syringe) of these dye solutions are added to 5-mL aliquots of the subjects blood, giving final concentrations of the dye in blood beginning with 24 mg/liter, ...
In the investigators previous study of 51 breast reconstructions (32 patients), we compared three different assessments of vascular perfusion of tissue; clinical judgment, fluorescein dye angiography (FDA) and laser-assisted indocyanine green dye angiography (ICG). Because tissue with poor perfusion becomes necrotic and can compromise the success of breast reconstruction, it is important that tissue with poor perfusion be removed at the time of the reconstructive surgery. However, it is also important to remove the least amount of potentially necrotic tissue as possible so that the breast reconstruction is not compromised by lack of skin. Therefore finding the best way to assess potentially necrotic tissue is a vital clinical question. In the initial study the 3 different assessment methods which were made at the time of surgery were compared to the subsequent development of necrotic tissue.. In the initial study, clinical judgment was the basis for determining the tissue removed because it had ...
TY - JOUR. T1 - Re. T2 - Is near infrared fluorescence imaging using indocyanine green dye useful in robotic partial nephrectomy: A prospective comparative study of 94 patients. AU - Klaassen, Zachary W A. AU - Ostrowski, Andrew. AU - Madi, Rabii Hussein. AU - Terris, Martha Kennedy. PY - 2013/3/1. Y1 - 2013/3/1. UR - http://www.scopus.com/inward/record.url?scp=84872951174&partnerID=8YFLogxK. UR - http://www.scopus.com/inward/citedby.url?scp=84872951174&partnerID=8YFLogxK. U2 - 10.1016/j.eururo.2012.12.009. DO - 10.1016/j.eururo.2012.12.009. M3 - Comment/debate. C2 - 23357893. AN - SCOPUS:84872951174. VL - 63. SP - 581. EP - 582. JO - European Urology. JF - European Urology. SN - 0302-2838. IS - 3. ER - ...
TY - JOUR. T1 - Frozen Section for Detection of Lymph Nodes After Cervical Injection with Indocyanine Green (ICG) for Sentinel Lymph Node Technique in Endometrial Cancer Staging. AU - Casarin, Jvan. AU - Multinu, Francesco. AU - Pasupathy, Kalyan S. AU - Weaver, Amy. AU - McGree, Michaela. AU - Tortorella, Lucia. AU - Torres, Diogo. AU - Kumar, Amanika. AU - Langstraat, Carrie. AU - Huang, Yajue. AU - Ghezzi, Fabio. AU - Mariani, Andrea. AU - Glaser, Gretchen. PY - 2018/1/1. Y1 - 2018/1/1. N2 - Objective: The aim of this study was to assess the role of frozen section (FS) in identifying an absence of lymph nodes during sentinel lymph node (SLN) biopsy for apparent early-stage endometrial cancer (EC). Methods: Consecutive apparent early-stage EC patients who had SLNs removed after cervical injection with indocyanine green (ICG) from 1 June 2014 to 30 June 2016 were analyzed. An empty node (EN) was defined as an SLN specimen without evidence of lymph node(s). The association of tumor and patient ...
TY - JOUR. T1 - The Intra-Operative Use of Fluorescence Angiography in the Management of Ischemic Bowel. AU - Boyer, Kryston AU - Diener, Brian. PY - 2017/10/16. Y1 - 2017/10/16. N2 - Fluorescence angiography (FA) utilizing indocyanine green (ICG) has been used for decades to assist with ophthalmic operations¹. Recently, its use has become more common in the management of myocutaneous flaps, ostomy and intestinal anastomoses. The use of ICG dye angiography in determining the viability of bowel and how it affects the surgeons management is relatively unknown. Below is a case of a 63-year-old male who presented in the intensive care unit (ICU) with an acute abdomen concerning for ischemic bowel. Fluorescence angiography was a key adjunct for the operative decision making for this patient.. AB - Fluorescence angiography (FA) utilizing indocyanine green (ICG) has been used for decades to assist with ophthalmic operations¹. Recently, its use has become more common in the management of myocutaneous ...
TY - JOUR. T1 - Targeted extracranial-intracranial bypass with intra-aneurysmal administration of indocyanine green. T2 - Case report. AU - Bain, Mark D.. AU - Moskowitz, Shaye I.. AU - Rasmussen, Peter A.. AU - Hui, Ferdinand. PY - 2010/12. Y1 - 2010/12. N2 - BACKGROUND AND IMPORTANCE: Early origin of the middle cerebral artery M2 segment is a normal variant. When such a vessel is occluded proximally, the parenchyma distal to the vessel may become ischemic. Targeted extracranial to intracranial bypass to such a specific branch may preserve perfusion to the end organ. We describe the use of intra-aneurysmal injection of indocyanine green to identify a target middle cerebral artery branch (MCA) for bypass, immediately followed by proximal parent vessel sacrifice via endovascular embolization. CLINICAL PRESENTATION: A 45-year-old woman presented to an outside hospital with headaches. Magnetic resonance imaging revealed a giant aneurysm of the right MCA. The aneurysm gave rise to an M2 branch that ...
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The decision to re-operate on a potentially ischemic free flap remains challenging. Indocyanine green videoangiography (ICG) with the FLOW(®) 800 tool is a method which allows an immediate qualitative conclusion about the patency of an anastomosis. Is it also able to predict the outcome of potentially compromised vascular free flaps?An epigastric flap was raised and repositioned in 79 rats. Intraoperative fluorescence angiography was performed using ICG videoangiography and the FLOW(®) 800 tool was applied. Six regions of interest were positioned systematically over the flap, changes of the ICG fluorescence were color coded with respect to time and 474 measurements were performed. The flap was clinically monitored for one week and the resulting necrotic areas were correlated with the ICG/FLOW(®) 800 results.Mean intensity of clinically vital areas was 83.39 ± 50.96 arbitrary units (AU) and 37.33 ± 15.14 AU in necrotic areas. The receiver operating characteristic curve and Youden-Index ...
Using near-infrared spectroscopy (NIRS) and the tracer indocyanine green (ICG), we quantified blood flow in calf muscle and around the Achilles tendon during plantar flexion (1-9 W). For comparison, blood flow in calf muscle was determined by dye dilution in combination with magnetic resonance imaging measures of muscle volume, and, for the peritendon region, blood flow was measured by (133)Xe washout. From rest to a peak load of 9 W, NIRS-ICG blood flow in calf muscle increased from 2.4+/-0.2 to 74+/-5 ml x 100 ml tissue(-1) x min(-1), similar to that measured by reverse dye (77+/-6 ml x 100 ml tissue(-1) x min(-1)). Achilles peritendon blood flow measured by NIRS-ICG rose with exercise from 2.2+/-0.5 to 15.1+/-0.2 ml x 100 ml(-1) x min(-1), which was similar to that determined by (133)Xe washout (2.0+/-0.6 to 14.6+/-0.3 ml x 100 ml tissue(-1) x min(-1)). This is the first study using NIRS and ICG to quantify regional tissue blood flow during exercise in humans. Due to its high spatial and ...
PURPOSE To investigate the possible persistence and characteristics of infrared fluorescence of the fundus for several months after surgery with intraocular injection of indocyanine green (ICG). DESIGN Interventional, noncomparative, prospective case series. PARTICIPANTS Seventeen patients operated on in our department with ICG injection into the vitreous cavity, who gave prior informed consent. METHODS After standard three-port pars plana vitrectomy and posterior vitreous detachment, 0.1 to 0.2 ml of an ICG solution at a concentration of 2.5 mg/ml was injected through a 5- micro m sterile filter over the posterior pole and left in place for 3 minutes. The stained internal limiting membrane was then peeled off. Patients had postoperative infrared fundus photographs at each consultation in our department. Follow-up ranged from 1 to 7 months. Visual acuity and any unexpected event were also recorded. MAIN OUTCOME MEASURES Postoperative infrared fluorescence of the fundus. RESULTS The day after
Figure 1. (A) The ICG molecule has biophysical properties that make it useful for visualizing the choroidal circulation. (B, C and D) These properties make it possible to visualize choroidal neovessels beneath blood layers, exudates or RPE detachments (PEDs) by differentiating (in the latter case) between the hypofluorescent serous component and the hyperfluorescent vascular component. With FA, both the serous and the vascular component appear hyperfluorescent, and the limits of CNV may be masked as a result. A and B. Visualization of the choroidal circulation under thin blood layers. (C) Hot spots visible in an area of exudation. (D) PED with small CNV at the margin of the lesion. With FA, both the serous and the vascular component appear hyperfluorescent, while with ICGA only the vascular component of PED appears hyperfluorescent.. The most important contribution of ICGA to the study of CNV in AMD is its capacity to detect choroidal new vessels which in some cases remain occult when using ...
Surgery is the most effective method to cure patients with solid tumors. New techniques in near-infrared (NIR) cancer imaging are being used to identify surgical margins and residual tumor cells in the wound. Our goal was to determine the optimal time and dose for imaging solid tumors using Indocyanine Green. Syngeneic murine flank tumor models were used to test NIR imaging of ICG at various doses ranging from 0 to 10 mg/kg. Imaging was performed immediately after injection and up to 72 hours later. Biodistribution in the blood and murine organs were quantified by spectroscopy and fluorescence microscopy ...
Indocyanine green (ICG) has been reported as a potential near-infrared (NIR) photosensitizer for photodynamic therapy (PDT) of cancer. However the application of ICG-mediated PDT is both intrinsically and physiologically limited. Here we report a combination of ICG-PDT with a chemotherapy drug etoposide (VP-16), aiming to enhance the anticancer efficacy, to circumvent limitations of PDT using ICG, and to reduce side effects of VP-16. We found in controlled in vitro cell-based assays that this combination is effective in killing non-small-cell lung cancer cells (NSCLC, A549 cell line). We also found that the combination of ICG-PDT and VP-16 exhibits strong synergy in killing non-small-cell lung cancer cells partially through inducing more DNA double-strand breaks (DSBs), while it has a much weaker synergy in killing human normal cells (GM05757). Furthermore, by studying the treatment sequence dependence and the cytotoxicity of laser-irradiated mixtures of ICG and VP-16, we found that the observed synergy
[IMG] Author: Mitsuo Kusano (Editor), Norihiro Kokudo (Editor), Masakazu Toi (Editor), Masaki Kaibori (Editor) Full Title: ICG Fluorescence Imaging...
Infectious pneumonia is a major cause of morbidity/mortality, mainly due to the increasing rate of microorganisms resistant to antibiotics. Photodynamic Inactivation (PDI) is emerging as a promising treatment option, which effects are based on oxidative stress, targeting several biomolecules and probably preventing potential resistant strains. In previous studies, the in vitro inactivation of Streptococcus pneumoniae using indocyanine green (ICG) and infrared (IR) light source (780 nm) was successful, and achieving satisfactory reduction of colony-forming units (CFU/mL). In the present study, a proof-of-principle protocol was designed to treat lung infections by PDI using extracorporeal irradiation with a 780 nm laser device and nebulized ICG as photosensitizer. Balb/c mice were infected with S. pneumoniae and PDI was performed two days after infection using 800 μM of nebulized ICG and extracorporeal irradiation. Our results indicate that IR-extracorporeal PDI using nebulized ICG may be ...
All the patients undergoing LC between June 2012 and July 2013 were asked to participate in this institutional review board (IRB) approved study. Fluoroscopic and fluorescent cholangiographies were performed to delineate the extrahepatic biliary ducts. For the FC a single dose of 0.05 mg/kg of indocyanine green (ICG) was administered intravenously one hour prior to surgery. During the procedure, alternate exposure from xenon to infrared lights was used to identify the biliary structures before and after dissection. A questionnaire to assess the surgeons visibility of extrahepatic bile ducts was administered.. Results ...
Nanoparticles formulated from biodegradable polymers such as polyethylene glycol (PEG) with molecular weight 6000 and 15,000 respectively are being extensively investigated as drug (Indocyanine green, ICG) delivery system due to their controlled release characteristics and biocompatibility.
Application of dynamic indocyanine green perfusion imaging for evaluation of vasoactive effect of acupuncture: a preliminary follow-up study on normal healthy volunteers Yuri An,1 Jong Wook Jeon,2 Kihwan Kwon,3 Chulhee Choi1 1Department of Bio and Brain Engineering, KAIST, Daejeon, South Korea; 2Acupuncture, Moxibustion and Meridian Research Centre, Korea Institute of Oriental Medicine, Daejeon, South Korea; 3Department of Cardiology, College of Medicine, Ewha Womans University, Seoul, Korea Background: Even though acupuncture has long been used for alleviating symptoms related to vascular insufficiency, the clinical effect of acupuncture on peripheral circulation has not been fully confirmed. In this study, we investigated whether a near-infrared optical imaging-based method can be used to evaluate the efficacy of the acupuncture procedure to induce changes in peripheral tissue perfusion. Methods: Two normal, healthy controls were treated with acupuncture on two acupoints (LI-4 and SI-3) three times
To the Editor: We read with great interest the recent article by Nussbaum et al on the application of indocyanine green angiography to delineate venous system before dural opening in the parasagittal region. Although we agree with the authors that indocyanine green angiography is useful to localize veins or sinuses through the dura mater, we already…
Thus far, it is not entirely clear if single vessels of CNV lesions completely vanish with anti-VEGF treatment or just become undetectable by OCTA using the SSADA algorithm.21,32 The OCTA systems interscan time may be a reason for undetectable flow and consequently vessels and CNV-A. Long interscan times, which are not achieved with the commercially available SD-OCTA system used in this study, would be needed to detect very slow blood flow. For example, ultrahigh speed swept-source (SS) OCTA systems with motion correction are needed to compensate for eye motion artifacts leading to decorrelation noise. Previous studies have reported statistically significantly larger CNV demarcation areas for different SS-OCTA than for commercially available SD-OCTA systems.33,34 Further, an evaluation algorithm (e.g., variable interscan time analysis) as described by Choi et al., allows the interscan time to be increased from ∼1.5 to 3 ms, which decreases the limits for the lowest detectable and fastest ...
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It is well established that immunologic memory generated early in life can be maintained into old age and mediate robust anamnestic antibody responses. cell responses were strongly reduced in the elderly and displayed an excellent correlation with Ab titres. The purchase Indocyanine green data suggest that the dramatically lower antibody response in older people could only partly end up being accounted for with the decreased B cell quantities and was highly correlated with deep functional flaws in Compact disc4 help. within the grouped family for 10?min and stored in ?20C. Limiting-dilution evaluation of storage B cells An ELISA-based limiting-dilution assay (LDA) was purchase Indocyanine green useful for enumeration of TBEV-specific and total IgG storage B cells, essentially as defined previously (Amanna and Slifka 2006). Quickly, purified B cells had been resuspended in RPMI-1640 lifestyle moderate purchase Indocyanine green supplemented with 10% fetal leg serum, 2?mM l-glutamine and 100?U/ml ...
Purpose : To compare swept source OCT enface choroidal vasculography with ICG angiography. Methods : In this retrospective study, 9mm x 9mm OCT angiography examinations with 512 x 512 A-scans were performed using a commercially available (outside of the US) swept source OCT instrument using a 1050 nanometer wavelength. Standard fluorescein and ICG angiography was performed on each patient. The OCT-A of each patient was compared to the FA. The ICG was compared to inverted and re-segmented OCT slabs of the structural data inherent in the OCT-A data set. Multiple slabs at different depths in the choroid were evaluated. Results : 5 cases of patients with CSCR were retrospectively examined for this study. In each case the correlation between ICG and enface choroidal vasculaography was considerable. All vessels in the ICG were also visible in the choroidal vasculography with the added benefit that there was depth resolution in the vasculography. Conclusions : ...
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Johns Hopkins School of Medicine Distinguishing normal postoperative inflammation from infectious processes can be challenging, and relies largely upon interpretation of clinical parameters and microbiologic data. An imaging modality that assesses variations in leukocytic infiltration could provide more specific information without the delays inherent in culture. Autologous monocytes loaded with the only FDA-approved near infrared fluorophore, indocyanine green (ICG), may be injected intravenously and homing assessed non-invasively using a clinically-applicable near infrared laser to investigate cutaneous inflammatory processes. RAW 264.7 mouse monocytes were coincubated with ICG solution. Fluorescence was confirmed microscopically. Homing ability of loaded cells was assessed in vitro using a microplate chemotaxis assay. Labeled cells were injected systemically into mice with induced sterile inflammation (Complete Freund s Adjuvant inoculation) or infection (Group A Streptococcus inoculation) of ...
The Spectropen, a hand-held device developed by Emory and Georgia Tech scientists, was designed to help surgeons see the margins of tumors during surgery.. Some of the first results from procedures undertaken with the aid of the Spectropen in human cancer patients were recently published by the journal PLOS One. A related paper discussing image-guided removal of pulmonary nodules was just published in Annals of Thoracic Surgery.. To test the Spectropen, biomedical engineer Shuming Nie and his colleagues have been collaborating with thoracic surgeon Sunil Singhal at the University of Pennsylvania.. As described in the PLOS One paper, five patients with cancer in their lungs or chest participated in a pilot study at Penn. They received an injection of the fluorescent dye indocyanine green (ICG) before surgery.. ICG is already FDA-approved for in vivo diagnostics and now used to assess cardiac and liver function. ICG accumulates in tumors more than normal tissue because tumors have leaky blood ...
Aiming at the identification of latest fluorescent reporters for qualified optical probes, we assessed the application-applicable functions of the novel asymmetric cyanine, DY-681, compared to the sole clinically permitted dye indocyanine green (ICG), the golden imaging common Cy5.five, as well as the asymmetric cyanine DY-676 effectively exploited by us for the design of various distinction brokers. This comparison included the see analysis in the spectroscopic Houses of your totally free fluorophores and their thermal balance in aqueous Resolution and their cytotoxic potential ...
This book represents a head-to-toe guide for clinicians who are interested in applying real-time NIR imaging for their patients. Expert surgeons from around the globe share their experience with NIR imaging, most commonly performed using indocyanine green (ICG) fluorescence.
An angiogram may be recommended by your Ophthalmologist if any irregularities of the retina are discovered at your consultation. The photographs can then help your specialist make a diagnosis. Ongoing angiograms may also be performed for certain eye diseases to monitor any changes over a period of time.. Age-related Macular Degeneration (AMD) affects millions of people and gradually destroys the sharp, central vision. Central vision is needed for seeing objects clearly and for common daily tasks such as reading and driving. AMD affects the macula, the part of the eye that allows you to see fine detail. Angiograms are important for showing any leaking blood vessels in the macula and can show up any small leaks that could not otherwise be detected.. Angiograms are also beneficial in discovering any leaking blood vessels in the eye associated to Diabetes. Diabetes is a disease in which the body does not properly control the amount of sugar in the blood. Regular eye checks are needed for diabetics, ...
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摘要:Graphene oxide (GO), as a drug delivery carrier, has attracted considerable attention because of its interesting properties. However, GO tends to aggregate in aqueous solution. Amphiphilic molecules are usually necessary to stabilize GO. The introduction of these non-functional macromolecules on the one hand reduces drug loading, but on the other hand may cause unpredictable side effects. This study proposes a new strategy for stabilizing GO with a functional photothermal agent, IR820 (new indocyanine green) derivative. IR820 derivative results from the conjugation of active targeted lactobionic acid (LA) with IR820 for the formation of IR820-LA. IR820-LA features central aromatic groups that can associate with the GO basal plane through pi-pi interactions. The flanking moiety of hydrophilic LA and sulfonic groups thus provides steric stabilization of GO in aqueous solution. Moreover, IR820-LA endows GO/doxorubicin (GO/DOX) nanovehicles with fluorescence imaging ability and actively ...
Photothermal therapy is a local treatment method for cancer and the heat energy generated from it could destroy the tumor cells. This study is aimed to investigate the temperature distribution in tumor tissue and surrounding health tissue of tumor bearing mice applying mathematical simulation model. Tumor bearing mice treated by laser combined with or without indocyanine green. Monte Carlo method and the Pennes bio-heat equation were used to calculate the light distribution and heat energy. COMSOL Multiphysic was adopted to construct three dimensional temperature distribution model. This study revealed that the data calculated by simulation model is in good agreement with the surface temperature monitored by infrared thermometer. Effected by the optical parameters and boundary conditions of tissue, the highest temperature of tissue treated by laser combined with indocyanine green was about 65 °C which located in tumor tissue and the highest temperature of tissue treated by laser was about 43 °C which
Photothermal therapy is a local treatment method for cancer and the heat energy generated from it could destroy the tumor cells. This study is aimed to investigate the temperature distribution in tumor tissue and surrounding health tissue of tumor bearing mice applying mathematical simulation model. Tumor bearing mice treated by laser combined with or without indocyanine green. Monte Carlo method and the Pennes bio-heat equation were used to calculate the light distribution and heat energy. COMSOL Multiphysic was adopted to construct three dimensional temperature distribution model. This study revealed that the data calculated by simulation model is in good agreement with the surface temperature monitored by infrared thermometer. Effected by the optical parameters and boundary conditions of tissue, the highest temperature of tissue treated by laser combined with indocyanine green was about 65 °C which located in tumor tissue and the highest temperature of tissue treated by laser was about 43 °C which
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PubMed comprises more than 30 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
Fluorescence lifetime in heterogeneous multiple light scattering systems is analyzed by an algorithm without solving the diffusion or radiative transfer equations. The algorithm assumes that the optical properties of medium are constant in the excitation and emission wavelength regions. If the assumption is correct and the fluorophore is a single species, the fluorescence lifetime can be determined by a set of measurements of temporal point-spread function of the excitation light and fluorescence at two different concentrations of the fluorophore. This method is not dependent on the heterogeneity of the optical properties of the medium as well as the geometry of the excitation-detection on an arbitrary shape of the sample. The algorithm was validated by an indocyanine green fluorescence in phantom measurements and demonstrated by an ...
Table 1: Selection of Fluorescent Peptides in Development for Imaging Product Candidates BLZ100 is a tumor paint being developed by Blaze Bioscience for use as an imaging agent for cancer surgery involving a range of cancer types including brain, breast, prostate, lung, colorectal, skin and sarcomas. The company aims to provide surgeons with the ability to see cancer cells at a high resolution throughout surgery and thereby provide better detection and allow for the precise removal of cancer. The tumor paint consists of chlorotoxin, a tumor penetrating peptide, conjugated to indocyanine green dye. In April 2016, Blaze completed a Phase I study of BLZ100 in adult subjects with glioma undergoing surgery (1). Blaze announced interim clinical data from this Phase I study in November 2015 and the data suggested that BLZ100 is well tolerated and tumor-specific fluorescence can be achieved in vivo. The product is currently in multiple Phase I proof-of-concept clinical studies (2). Avelas Biosciences is ...
Background: Dual localization methods with blue dye and radioisotope are commonly employed for SLN identification but potential drawbacks include allergic reactions, radiation exposure and mandatory licencing. Identification rates exceeding 95% have been reported using the fluorescent tracer indocyanine green (ICG) as a navigation system. A feasibility study was undertaken to confirm the sensitivity and safety of ICG as a tracer agent for sentinel lymph node (SLN) identification in early breast cancer.. Methods: In an open, non-randomised study, 100 clinically node negative patients (95 unilateral; 5 bilateral) scheduled to undergo routine SLN biopsy for core-biopsy proven invasive breast cancer were identified at the multidisciplinary team meeting [53 screen-detected; 51 symptomatic]. All patients received triple localization with blue dye (2.5% Patent Blue), radiocolloid and ICG (0.5%). The number of sentinel nodes for each patient were recorded numerically and whether blue, radioactive or ...
Seeking an alternative, Holt, Singhal and colleagues turned to near-infrared, or NIR, imaging. They chose to test the only Food and Drug Administration-approved contrast agent for NIR, a dye called indocyanine green, or ICG, that fluoresces a bright green under NIR light. ICG concentrates in tumor tissue more than normal tissue because the blood vessels of tumors have so-called "leaky" walls from growing quickly.. "Since 1958 when ICG was initially FDA approved, it has been used to examine tissue perfusion and clearance studies," Singhal said. "However, our group has been experimenting with new strategies to use ICG to solve a classic problem in surgical oncology: preventing local recurrences. Our work uses an old dye in a new way.". To see if visualizing ICG under NIR could help them define cancerous from non-cancerous tissues, the Penn-led team first tested the approach in mice. They administered ICG to mice with a type of lung cancer and found that they could use NIR to distinguish tumors ...
The goal of this clinical research study is to find lymph nodes before surgery using a dye called indocyanine green (IC-Green). Objectives: 1. To determine the...
Video articles in JoVE about indocyanine green include Detecting Abnormalities in Choroidal Vasculature in a Mouse Model of Age-related Macular Degeneration by Time-course Indocyanine Green Angiography, Near-infrared Navigation System for Real-time Visualization of Blood Flow in Vascular Grafts, Fluorescence-quenching of a Liposomal-encapsulated Near-infrared Fluorophore as a Tool for In Vivo Optical Imaging, Fluorescent Dye Labeling of Erythrocytes and Leukocytes for Studying the Flow Dynamics in Mouse Retinal Circulation, An
Targeted therapies for cancer are often challenging because cancer cells are the same in chemical makeup to their non-mutated counterparts. However, their mechanical properties differ slightly. Cancer tumors form abnormally and lack lymphatic drainage. Our research focuses on cancer imaging and therapy using near infrared (NIR) erythrocyte-mimicking transducer (NETs) nanoparticles that take advantage of this enhanced permeability and retention effect (EPR) in tumors. When excited by NIR light, NETs transduces this energy to emit fluorescence, generate heat, and produce radical oxygen species (ROS). The fluorescence from NETs can be used for imaging tumors while the heat and ROS production can be used for photothermal and photodynamic therapies, respectively. These constructs are made by taking red blood cells, depleting them of hemoglobin, extruding them into nano-sized particles, then doping them with the only FDA-approved NIR chromophore, indocyanine green (ICG). Results of flow cytometry and
Near infrared fluorophore useful for non-invasive in vivo imaging. Absorption properties are similar to indocyanine green (ICG), but possesses higher fluorescence quantum yield.
This paper considers some issues pertinent to laser welding of elastin-based biomaterials to tissues using a pulsed diode laser (lO-is pulse) and indocyanine green (ICG) as an absorbing chromophore to localize laser heating to the "weld surface", the elastin/tissue interface where welding occurs. Experiments involved laser welding of elastin heterographs to the intimal surface of the carotid artery (in vitro, porcine) as a -4x5 spotweld, then determining the breaking strength when the two tissues were pulled in a direction parallel to the plane of the spot weld while submerged in water. The questions answered are: . WHAT IS THE PEAK TEMPERATURE REQUIRED FOR WELDING ELASTIN HETEROGRAPH TO THE INTIMAL SURFACE OF CAROTID ARTERY? ANSWER: 3OO °C threshold, -6OO °C for maximum strength. This estimate is based on optical measurements of dye accumulation in stain layer and measurements of thickness of stain layer via fluorescence microscope examination. . WHATIS THE DEPENDENCE OF WELD STRENGTH ON THE ...