Bacterial inclusion bodies are submicron protein clusters usually found in recombinant bacteria that have been traditionally considered as undesirable products from protein production processes. However, being fully biocompatible, they have been recently characterized as nanoparticulate inert materials useful as scaffolds for tissue engineering, with potentially wider applicability in biomedicine and material sciences. Current protocols for inclusion body isolation from Escherichia coli usually offer between 95 to 99% of protein recovery, what in practical terms, might imply extensive bacterial cell contamination, not compatible with the use of inclusion bodies in biological interfaces. Using an appropriate combination of chemical and mechanical cell disruption methods we have established a convenient procedure for the recovery of bacterial inclusion bodies with undetectable levels of viable cell contamination, below 10-1 cfu/ml, keeping the particulate organization of these aggregates regarding size
Bacterial inclusion bodies are microscopic, ovoid-shaped aggregates of insoluble protein. Under protease exposure a digestion process is produced that reveals a variable fragmentation rate, not compatible with a surface restricted erosion of body particles, or an uniform sensibility to the fragmentation agent. The modelling and fitting of experimental data is performed in two steps. (a) Due to poor estimation of protein amounts only first derivatives can be numerically evaluated, and a non-linear first-order fragmentation model is adopted. Although it is a very good approximation for intermediate points, the asymtotic behaviour of the solution is inconsistent with the fragmentation process. (b) The solution of previous kinetic modelling is used to compute higher-order derivatives in intermediate points and to adopt a higher-order lineal model for the overall interval with protein fragmentation. The resulting model consists in a superposition of Poisson processes associated with several ...
How to Avoid Bacterial Inclusion Bodies - Download as PDF File (.pdf), Text File (.txt) or read online. Study about production of recombinant GFP by Escherichia coli.
Escherichia coli that has been genetically manipulated by recombinant DNA technology to synthesize human insulin polypeptides (A chain, B chain, or proinsulin) contains prominent cytoplasmic inclusion bodies. The amount of inclusion product within the cells corresponds to the quantity of chimeric protein formed by the bacteria. At peak production, the inclusion bodies may occupy as much as 20 percent of the Escherichia coli cellular volume. ...
The cytoplasmic aggregation of TAR DNA-binding protein-43 (TDP-43) is a hallmark of degenerating neurons in amyotrophic lateral sclerosis (ALS) and subsets of frontotemporal dementia (FTD). In order to reduce TDP-43 pathology, we generated single-chain (scFv) antibodies against the RNA recognition motif 1 (RRM1) of TDP-43, which is involved in abnormal protein self-aggregation and interaction with p65 NF-κB. Virus-mediated delivery into the nervous system of a scFv antibody, named VH7Vk9, reduced microgliosis in a mouse model of acute neuroinflammation and mitigated cognitive impairment, motor defects, TDP-43 proteinopathy, and neuroinflammation in transgenic mice expressing ALS-linked TDP-43 mutations. These results suggest that antibodies targeting the RRM1 domain of TDP-43 might provide new therapeutic avenues for the treatment of ALS and FTD.. ...
Protein inclusions are a predominant molecular pathology found in numerous neurodegenerative diseases, including amyotrophic lateral sclerosis and Huntingtons disease. Protein inclusions form in discrete areas of the brain characteristic to the type of neurodegenerative disease, and coincide with the death of neurons in that region (e.g. spinal cord motor neurons in amyotrophic lateral sclerosis). This suggests that the process of protein misfolding leading to inclusion formation is neurotoxic, and that cell-autonomous and non-cell autonomous mechanisms that maintain protein homeostasis (proteostasis) can, at times, be insufficient to prevent protein inclusion formation in the central nervous system. The heat shock response is a pro-survival pathway induced under conditions of cellular stress that acts to maintain proteostasis through the up-regulation of heat shock proteins, a superfamily of molecular chaperones, other co-chaperones and mitotic regulators. The kinetics and magnitude of the heat shock
Antibodies: A Laboratory Manual, has been revised, extended, and updated by Edward Greenfield of the Dana-Farber Cancer Center, with contributions from other leaders in the field. This second edition of the manual is an essential resource for molecular biology, immunology, and cell culture labs on all matters relating to antibodies.
Inclusion bodies (IBs) are intracellular, insoluble protein aggregates, commonly observed when a protein of interest is expressed at high concentrations in a bacterial cell-based expression system. The molecular determinants of IB formation are poorly understood, and are of both fundamental and biotechnological significance. The stability, folding, and structure of the tenth human fibronectin type III domain (10Fn3) have been studied previously, making it an attractive model system to investigate IB formation. A library of 10Fn3 mutants was provided by Bristol-Myers Squibb; 31 of these mutants were expressed in Escherichia coli and analyzed. The percentage of the expressed protein found within IBs was quantified at different expression time points using densitometric analysis of soluble and inclusion body (insoluble) cell lysate fractions separated by centrifugation and subjected to polyacrylamide gel electrophoresis. Although most of these mutants differ from each other in only 3 amino acid ...
The induction of recombinant protein expression is effected by addition of the non-metabolisable lac-inducer IPTG. Although the process is simple in theory, the BL21 cells tend to form inclusion bodies very easily. This reduces recovery dramatically and therefore in the past, each recombinant protein had to be assessed individually for their solubility and expression. This would involve optimising the length of IPTG induction and the temperature, i.e. shorter induction times and lower temperatures are considered best for maintaining recombinant solubility. However, the following two papers have shown that the existing idea that inclusion bodies are dead-end products is incorrect and, with the correct treatment, can be resolubilised, refolded and recovered as active protein. This therefore has the potential to increase recovery and do away with laborious time/temp optimisations for each recombinant protein.. ...
From May-Hegglin Anomaly, eMedicine, 2008. Figure 6 2000x blood smear of a MHA patient demonstrating a typical giant platelet with ill defined granulation. A normal sized platelet is also seen here. The cytoplasmic inclusion body represents a Dohle body.. A cell blood count is essential in starting a workup in these patients. The platelet count is decreased, usually between 40,000-80,000mm3. The platelets are enlarged up to 15mm3 in diameter, with normal morphology. [4] Evaluation at the electron microscopy level reveals normal cell organelles with an increased amount of disorganized microtubuli.. The Wright-stained peripheral blood smear shows cytoplasmic inclusion bodies, most dominant in the neutrophils, but some are present in the eosinphils, monocytes, and basophils.. The inclusions are up to 5µm in size, they are spindle shaped, pale, blue-staining bodies that consist of ribosomes, endoplasmic reticulum, and microfilaments. [4] The inclusions are similar to Döhle bodies and are found in ...
The late chlamydial inclusion membrane is not derived from the endocytic pathway and is relatively deficient in host proteins.: Chlamydiae are obligate intracel
Prispelo je še nekaj vprašanj na temo "seminarji". N. Horvat sprašuje o citoplazemskih vključkih: " ... so to hraniva, ki jih shranjujejo nekatere bakterije v obliki zrnc v citoplazmi...". Odg.: pojem je širši in ne vključuje zgolj rezervnih snovi v celici (primer: zračni mešički). Vpr.: "Ali naslednji izrazi: znotrajplazemski vkljucki, citoplazemska zrna, metahromatska zrna, pomenijo isto kot citoplazmatski vkljucki?" Odg.: ...v glavnem gre za primerljive izraze, predlagam pa da se poenotimo na enem in ustrezen se mi zdi "citoplazemski vključki". Vpr.: " Ali pomeni izraz v anglescini cytoplasmic inclusion bodies - citoplazmatski vkljucki?" Odg.: ...da, to je angleški sinonimni pojem.. ...
Extracellular fibrous amyloid deposits or intracellular inclusion bodies containing abnormal protein fibrils characterize many neurodegenerative disorders, including Alzheimers disease. The burden of evidence suggests that the accumulation of protein aggregates plays a seminal role in pathogenesis. We have been at the forefront of studies on the role of soluble Aß oligomers in AD and are committed to understanding the mechanisms of Aß toxicity and the structure of noxious Aß assemblies. We are also actively investigating the involvement of the prion protein in AD and how Aß causes changes in tau metabolism. In particular, we are excited by emerging data that suggest certain forms of tau are secreted from neurons by unconventional mechanisms and that this may contribute to the stereotypic spread of tangle pathology in AD. This work has the potential not only to identify new drug targets for the treatment of AD, but also to identify novel biomarkers.. Different Aß assembly forms may mediate ...
... nologies has considerable experience with developing E. coli processes including cell culture, inclusion body production and inclusion body (protein) isolation and purification. This Separation and clarification of inclusion bodies WORKS ® Optimization Procedure is intended provide an efficient and economic alternative to centrifugation as an inclusion body recover procedure. To maximize the recovery of the inclusion bodies SFT can assist through its AT WORKS in developing the fermentation parameters to improve the inclusion body quality and size.. ...
DOBV RNA (strain DOB-90/5) was extracted from infected Vero E6 cells (CRL 1586; ATCC, USA). The genomic RNA of DOBV was reverse-transcribed and PCR amplified in order to generate the entire S-segment, using following oligonucleotide primers: 5-GCGAATTCGCAACACTAGAGGAACTCCAAAAGG-3 and 5-CGAAGCTTAGTGGTGGTGGTGGTGGTGAAGTTTGAGCGGCTCC-3. The amino-terminal part encoding the first 118 amino acids was generated using 5-CTGGCGCCTAACCGACGTGGTGGTGGTGGTGGTGATTCGAAGC-3 as reverse primer. In all constructs, a histidine (His) tag was introduced at the C-terminal end. PCR fragments were cloned in plasmids pTEX(rP40) or pTEXmp18 respectively with or without the inclusion of the P40 sequence in the construct. Following transformation of the E. coli ICONE 200 strain, the recombinant proteins were produced as intracellular inclusion bodies, recovered, and renatured. The recombinant proteins were purifi ed by metal chelate affinity chromatography using a HisTrap kit (Pharmacia, Puurs, Belgium). Using this ...
The demonstration of proteinaceous inclusions in the brain is the key step in the pathological diagnosis of degenerative dementias. The diversity of these diseases has necessitated the use of a panel of (immuno)stains to visualize all suspect pathologies, elevating diagnostic costs. Immunodetection of p62 (sequestosome 1), an abundant constituent in diverse pathological inclusions, holds the potential for a broad-specificity, high-contrast inclusion label. In the brain, pathological p62-positive aggregates comprise both cytoplasmic and nuclear types in neurones and glia, with abnormal tau, alpha-synuclein, TAR DNA-binding protein 43 or polyglutamine proteins as primary components. We therefore set out to evaluate the performance of p62 antibodies for diagnostic immunohistochemistry. We optimized the application conditions and compared the staining profiles of eight commercial p62 antibodies with each other and with reference immunostains, using 2-mm tissue multiarrays representing the major ...
Aggresome formation induced by Amblyomin-X.Cultured cells were treated with vehicle (PBS), 0.5 µM Ambly for 24 h, 5 µM MG-132 for 24 h, 3.5 µM CHX for 2 h or
Lot showed inclusion body (IB) and membrane fractions (M) of OPRM. doi:10.1371/journal.pone.0056500.gConfirmation of Full Length of OPRMOPRM, western blot
Hi, Does anyone recall a good review/protocol on purification of Inclusion Bodies from E. coli-expressed proteins?. Know that the specific steps depend on the protein in question but, is there any general approach? Please, E-mail me at hlucero at bu.edu Thanks ahead of time! Hector ...
Vázquez, E., Corchero, J. L., Burgueño, J. F., Seras-Franzoso, J., Kosoy, A., Bosser, R., Mendoza, R., Martínez-Láinez, J. M., Rinas, U., Fernández, E., Ruiz-Avila, L., García-Fruitós, E. and Villaverde, A. (2012), Functional Inclusion Bodies Produced in Bacteria as Naturally Occurring Nanopills for Advanced Cell Therapies. Adv. Mater., 24: 1742-1747. doi: 10.1002/adma.201104330 ...
This protocol describes how to re-dissolve the insoluble fraction of an E. coli protein expression for purification on His-Trap columns. It picks up where the expression protocol ends. See also: ...
Handsome producer つんく♂ / Tsunku looks to have added super-seiyuu Aya Hirano to his harem if their onstage antics are anything to go by; a jolly time looks to have been had in this promotional event for their upcoming collaboration, with Tsunku jolliest of all to have had Aya Hirano hanging off of him, by the looks of things.. The two are engaged in their first "collaboration", if a singers relationship with a producer could be called that, for the ED tune to ヒャッコ / Hyakko, the upcoming anime adaptation of a digitally distributed manga of the same name.. Read the rest of this entry ». ...
The Arc of Texas Inclusion Works! 2015 Conference would like to invite anyone in Region 13 or 20 who has not registered yet (Jan. 15th), to register for the Inclusion Works! 2015 Conference at the regular rate of $250. You will technically be registering late so your invoice will still say $275 (we cant change that), so please just indicate on your payment method or PO, ESC 13/20 special, and only pay $250 for teachers, principals or other education professionals. The parent/student rate remains $100. We have extended the registration deadline to Jan. 30th.. Register today for Inclusion Works! 2015. This year at Inclusion Works!. Wed. Feb. 4th ...
Bharat Financial Inclusion Offer Price, Bharat Financial Inclusion Stock Offer Price, Bharat Financial Inclusion IPO Offer Range, Bharat Financial Inclusion Listing on BSE/NSE, Issue Details, Initial Public Offer Details, Stock Performance, Competitors, Stock Quotes, Charts & more on The Economic Times
Infection with MARVPSAPmut results in compact inclusion bodies and accumulation of nucleocapsids in the periphery of cells.Huh-7 cells were infected with rMARVw
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186\186"Microscopically, extensive dystrophic changes and necroses of liver parenchyma are detected. In the nuclei of hepatocytes, basophilic or eosinophilic inclusion bodies are detected. The basophilic inclusion bodies are usually dense and occupy the entire nuclear inner space (arrow b), whereas the eosinophilic ones are round or irregularly shaped and surrounded by a light halo (arrow e). The diagnosis is based upon the typical gross lesions and the history records.A principal approach in IBH diagnostics is the histological investigation that helps to detect the intranuclear inclusion bodies. IBH should be distinguished mainly from IBD and chicken infectious anaemia (CIA). With regard to IBH prevention and control, the eggs of broiler parent flocks, where the disease is consecutively appearing in the progeny, should not be used for hatching. The access of wild birds should be prevented as they are potential carriers and distributors of the virus. The most important steps in IBH prevention ...
NIH Rare Diseases : 50 polyglucosan body disease affects the nervous system. individuals with this condition usually begin to show signs of the disorder after the age of 40. signs and symptoms include trouble walking due to decreased sensation in the legs (peripheral neuropathy) and muscle weakness and stiffness (spasticity). individuals may also have trouble controlling bladder function as a result of damage to the nerves of the bladder (neurogenic bladder). approximately half of the individuals with adult polyglucosan body disease also experience some degree of intellectual impairment. mutations in the gbe1 gene can cause adult polyglucosan body disease. in some cases, no mutation can be found and the cause of the disease is not known. adult polyglucosan body disease is thought to be inherited in an autosomal recessive manner. treatment usually involves a team of specialists who can address the specific symptoms such as walking difficulties, incontinence, and intellectual impairment. last ...
This paper concerns a disease affecting a group of African grey parrots, which involves intranuclear inclusion bodies composed of filamentous material. The disease was characterized by either sudden death or death within 2-3 days from onset of non-specific symptoms. At necropsy, gross lesions included enlarged liver, mild hepatic congestion and focal necrosis. Samples from five birds were fixed in 10% formol and routinely processed for light and electron microscopy. In four birds, numerous hepatocytes displayed an enlarged nucleus, with peripheral margination of chromatin; the nucleus was partially or wholly filled by a basophilic inclusion body. In the remaining bird, inclusion bodies were acidophilic and completely filled the nucleus; nuclear enlargement was less evident than in the other birds. At ultrastructural examination, and in both types of IIB, nuclei contained looped filaments but no evidence of viral structures. However, virion-like structures were observed in the cytoplasm of some ...
OMIM : 58 Adult polyglucosan body neuropathy is a late-onset, slowly progressive disorder affecting the central and peripheral nervous systems. Patients typically present after age 40 years with a variable combination of cognitive impairment, pyramidal tetraparesis, peripheral neuropathy, and neurogenic bladder. Other manifestations include cerebellar dysfunction and extrapyramidal signs. The pathologic hallmark of the disorder is the widespread accumulation of round, intracellular polyglucosan bodies throughout the nervous system, which are confined to neuronal and astrocytic processes (summary by Lossos et al., 1998). (263570) ...
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TY - JOUR. T1 - Curcumin abolishes mutant TDP-43 induced excitability in a motoneuron-like cellular model of ALS. AU - Dong, H.. AU - Xu, L.. AU - Wu, L.. AU - Wang, X.. AU - Duan, W.. AU - Li, H.. AU - Li, C.. PY - 2014/7/11. Y1 - 2014/7/11. N2 - Mutation of TAR DNA-binding protein-43 (TDP-43) is detected in familial and sporadic amyotrophic lateral sclerosis (FALS and SALS). TDP-43-positive cytoplasmic inclusions are present in both neuron and glia of ALS, although not in mutant Cu/Zn-superoxide dismutase (mSOD1)-related or RNA binding protein Fused in sarcoma (FUS)-related ALS. Previous studies have established that cortical hyper-excitability is common to both FALS and SALS patients. Much of our current understanding of neuron excitability has come from studying the subtype of mSOD1-related ALS. Thus, we evaluated the excitable capability through analyzing properties of action potentials (APs) and voltage-gated sodium (Na v ) channels on the cellular model, motoneuron-like cell lines that ...
FINAL DIAGNOSIS:. MOTOR NEURON DISEASE WITH BASOPHILIC INCLUSIONS. DISCUSSION:. Motor neuron disease (MND) is associated with a number of intracellular inclusion bodies within surviving motor neurons. In both sporadic and familial MND, Bunina bodies, skein-like inclusions and hyaline inclusions, some of which resemble Lewy bodies, may be seen (1). In Guam-type MND (2), post encephalitis lethargica-related MND (3) and occasional cases of familial MND with mutations in the SOD-1 gene (4), neurofibrillary tangles have been demonstrated. In addition, there have been a few reports (mainly in young individuals) of MND with basophilic cytoplasmic inclusions (5-11). MND with basophilic inclusions appears to be a distinctive histopathological subtype, characterized by circumscribed intraneuronal basophilic inclusions within motor neurons, but also widely distributed within the cerebral cortex, basal ganglia, brainstem and cerebellar nuclei. Unlike the more common MND-inclusions, these basophilic ...
Inclusion body disease (IBD) in the boid family of snakes, particularly Boa constrictor, has been recognized since the mid-1970s. It is so named because of the characteristic intracytoplasmic inclusions which are observed in clinical examinations in epidermal cells, oral mucosal epithelial cells, visceral epithelial cells, and neurons. In the 1970s and 1980s, the disease was most commonly observed in Burmese pythons, Python bivittatus. From the 1980s till the present day, it has been most commonly observed in boa constrictors from South America. All boid snakes should be considered susceptible to the disease. Many zoos quarantine boas specifically as a result of this high risk before introducing them into their permanent collections and (breeding) programs. While the disease has not been identified in non-boid snakes, it is yet unknown whether non-boid snakes harbour the virus. The primary host of this virus has not yet been identified, but mites are thought to be primary hosts or at least a ...
TY - JOUR. T1 - Cytoplasmic Inclusions (Cont.). AU - Valenzuela, Rafael. AU - Deodhar, Sharad D.. AU - Braun, William E.. AU - Banowsky, Lynn H.w.. PY - 1977/3/17. Y1 - 1977/3/17. N2 - To the Editor: We read with considerable interest the letter by Zyngier1 as well as his original article.2 The cytoplasmic crystalloid inclusions that he found within lung alveolar cells of mice infected with Toxocara canis are completely different from the nuclear and cytoplasmic fibrillary inclusions described by us in neutrophils trapped in human renal allografts.34 We do not think that they are the morphologic manifestation of the same pathologic process. Crystalloid inclusions similar to those illustrated in his letter have been found within the endoplasmic reticulum of both malignant and reactive plasma cells.5 6 7 8 Their biochemical nature has not been. No extract is available for articles shorter than 400 words.. AB - To the Editor: We read with considerable interest the letter by Zyngier1 as well as his ...
In addition to complete blood count (CBC) and blood smear evaluation, radiographs may also be useful in evaluation for possible lead toxicosis. Radiopaque gastrointestinal material is noted in approximately 20% of dogs with lead intoxication. Lead lines, due to precipitation of lead salts within the epiphyseal plate of long bones, is an uncommon finding. Postmortem histopathologic findings include cerebrocortical lesions (spongiosis, gliosis, neuronal necrosis, demyelination, vascular hypertrophy,) and intranuclear inclusion bodies (the intracellular storage form of lead) in hepatocytes or renal tubular epithelial cells). Inclusion bodies are pathognomonic for lead toxicosis.. Treatment of lead toxicosis consists first of symptomatic treatment (e.g. medical management of gastrointestinal signs and seizures) and evacuation of the gastrointestinal tract (via emesis, cathartics, enemas, endoscopic/surgical removal of lead objects). Antioxidants and thiol-containing drugs (e.g. Vitamin C, Vitamin E, ...
Inactive protein inclusion bodies occur commonly in Escherichia coli (E. coli) cells expressing heterologous proteins. Previously several independent groups have found that active protein aggregates or pseudo inclusion bodies can be induced by a fusion partner such as a cellulose binding domain from Clostridium cellulovorans (CBDclos) when expressed in E. coli. More recently we further showed that a short amphipathic helical octadecapeptide 18A (EWLKAFYEKVLEKLKELF) and a short beta structure peptide ELK16 (LELELKLKLELELKLK) have a similar property. In this work, we explored a third type of peptides, surfactant-like peptides, for performing such a
This research project attempted to grow and purify soluble Pseudomonas putida HMGR (PpHMGR) enzyme for the purpose of experimentation on its hypothesized morpheein protein behavior. Inclusion body formation has been the primary limiting factor in the growth and purification of soluble PpHMGR. Variation on incubation temperature, IPTG concentration, and growth time were explored in the growth phase, and imidazole concentration, presence of DTT, and presence of TCEP were explored in the purification phase. PpHMGR was grown in an E. coli host, cell lysis was performed by sonication, and subsequent purification was performed by nickel column elution FPLC. The purified product was characterized by FPLC, gel electrophoresis, and enzyme kinetics to determine the reduction of inclusion body formation and presence of soluble PpHMGR.
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B. A giant cell in this granuloma has a dark, intracellular inclusion within a vacuole. Inclusions like this are composed of calcium and iron, and may be associated with small, birefringent crystals of calcium oxalate, several of which are shown by polarized light in image C (arrow marks one). Both the inclusions and the crystals are thought to be produced endogenously by the cell. As the inclusions enlarge, they become lamellated and are called conchoid (resembling sea shells) or Schaumann bodies. These structures develop intracellularly, but they may become extracellular. They are not specific for any disease and may be found in any type of granuloma, although they are most common in HP and sarcoidosis. (Giemsa stain). ...
The effect of grain crushing during one dimensional compression and triaxial compression tests is investigated in a double-porosity granular material using the Discrete Element Method (DEM). Experimental tests results from the literature on a porous crushable soil (pumice sand) were simulated in 3-D using a large number of particles and without the use of agglomerates. The numerical simulations show the importance of incorporating internal porosity as a material characteristic. The variation of internal porosity with particle dimensions is of fundamental importance while describing the mechanical behavior of the material. The contact model parameters were calibrated using low confinement triaxial compression experimental tests while the particle failure criteria was calibrated using high pressure one dimensional compression tests. Particle strength size effect was assumed equal to that observed in other porous geomaterials and ultimately derived from the variation of internal porosity. The good ...
A granulating and coating apparatus in which a rotary drum containing powdery or granular material is rotated about the substantially horizontal axis for granulating and/or coating the powdery or granular material. The apparatus is provided with a disk valve mechanism for switching a gas supply or exhaust path to the rotary drum on the operating side of the rotary drum. The rotary drum is provided inside of itself with a baffle means for stirring powdery or granular material and a baffle displacing means for displacing the baffle means. Inside of the rotary drum is provided a temperature sensor for directly measuring a temperature of powdery or granular material. The rotary drum is composed of porous polygonal. Each side of the polygonal drum includes an outer enclosure mounted thereon and individual detachable.
The key pathological hallmarks of PD include the development of α-syn containing protein inclusions and DA neurodegeneration. Although it remains unclear whether mature α-syn aggregates or Lewy bodies are causative for PD, evidence suggests that factors that influence the misfolding and oligomerization of this polypeptide lead to neurotoxicity (13, 14). Regardless, proteins that play a role in protecting DA neurons from the degenerative loss associated with α-syn overproduction are candidate susceptibility markers as well as potential targets for therapeutic development. Here we have combined these distinct PD-associated phenotypic readouts to discern gene products with functional consequences for PD.. Among the gene products identified via this screen, a protein that demonstrated high neuroprotective capacity was C. elegans VPS41. VPS41 is highly conserved across species and has been best characterized in S. cerevisiae, where it is involved in trafficking from the trans-Golgi to the vacuole, ...
The cover image shows a paired helical filament of tau protein, which is the major component of tangles in Alzheimers disease. Tau filaments make up the protein inclusions seen inside nerve cells in the brains of people with many neurodegenerative conditions, including Alzheimers. In this issue, Sjors Scheres, Michel Goedert and their colleagues reveal the first high-resolution structures of the two forms of tau filaments in Alzheimers disease. The filaments were isolated from the brain of an individual with Alzheimers and imaged using cryo-electron microscopy. In both forms, the individual tau proteins form C shapes (white and blue), which stack together to form filaments. The high-resolution structures of these filaments may help the development of novel diagnostics and therapeutic compounds. Cover image: Thomas G. Martin ...
N.Schroten at med.ruu.nl (Niek Schroten) writes: ,Hi, ,Im trying to isolate a 103 kD protein in BL21(DE3) in inclusion ,bodies. Is anyone familiair with isolation of inclusion bodies in ,combination with a protein of this weight ? Does anyone have a useful ,protocol for inclusionbody isolation ? ,Thanking you in advance for your help, ,Niek Schroten. Biochemistry (1995)34:1858-1866 ibid (1995)34:5773-5794 ...
Composites with ferroelectric cubic inclusions are experimentally investigated with coaxial line technique in the microwave frequency range 1â€"18 GHz. The composites exhibit dispersive effective magnetic and dielectric properties. Strengthening of magnetic resonances with the increase in the inclusions’ number is observed. Electric resonances are damped out significantly due to material losses. The measurement results agree well with theoretical calculations based on Mie theory and Maxwellâ€"Garnett mixing relation. Even number and symmetric arrangement of the inclusions are needed to receive true measurement image in the whole investigated range. Composites utilizing dielectric resonators are attractive in electromagnetic metamaterials fabrication ...
Microvillus inclusion disease (also referred to as congenital microvillus atrophy) is, with Tuft enteropathy, the best known disease of the intestinal epithelium causing intractable diarrhea of infancy, and a leading cause of secretory diarrhea in the first weeks of life. A group of infants with a familial enteropathy characterized by protrac...
Microvillus inclusion disease (also referred to as congenital microvillus atrophy) is, with Tuft enteropathy, the best known disease of the intestinal epithelium causing intractable diarrhea of infancy, and a leading cause of secretory diarrhea in the first weeks of life. A group of infants with a familial enteropathy characterized by protrac...
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