Title:Immunophilins are Involved in the Altered Platelet Aggregation Observed in Patients with Type 2 Diabetes Mellitus. VOLUME: 20 ISSUE: 14. Author(s):E. Lopez, A. Berna- Erro, J.M. Hernandez-Cruz, G.M. Salido, P.C. Redondo and J.A. Rosado. Affiliation:Department of Physiology, University of Extremadura, Avd. Universidad s/n, 10003-Caceres (Spain).. Keywords:Aggregation, DM2, FKBP52, immunophilin, secretion, SOCE.. Abstract:Platelet hyperaggregability might contribute to vascular complications associated with type 2 diabetes mellitus (DM2).Experimental evidence supports a direct link between altered Ca2+ entry and hyperaggregability in DM2 patients. Objectives: We aimed to investigate whether altered immunophilin expression and function are involved in the abnormal Ca2+ entry observed in platelets from DM2 patients. Results: Inhibition of immunophilins by tacrolimus (FK506) and sirolimus (rapamycin) reduced Ca2+ entry in platelets from healthy donors and DM2 patients. Similarly, immunophilin ...
TY - JOUR. T1 - Targeting treatment-resistant breast cancer stem cells with FKBPL and Its peptide derivative, AD-01, via the CD44 pathway. AU - McClements, Lana. AU - Yakkundi, Anita. AU - Papaspyropoulos, Angelos. AU - Harrison, Hannah. AU - Ablett, Matthew P.. AU - Jithesh, Puthen V.. AU - McKeen, Hayley D.. AU - Bennett, Rachel. AU - Donley, Christopher. AU - Kissenpfennig, Adrien. AU - McIntosh, Stuart. AU - McCarthy, Helen O.. AU - ONeill, Eric. AU - Clarke, Robert B.. AU - Robson, Tracy. PY - 2013/7/15. Y1 - 2013/7/15. N2 - Purpose: FK506-binding protein like (FKBPL) and its peptide derivative, AD-01, have already shown tumor growth inhibition and CD44-dependent antiangiogenic activity. Here, we explore the ability of AD-01 to target CD44-positive breast cancer stem cells (BCSC). Experimental Design: Mammosphere assays and flow cytometry were used to analyze the effect of FKBPL overexpression/knockdown and AD-01 treatment ± other anticancer agents on BCSCs using breast cancer cell lines ...
Complete information for FKBPL gene (Protein Coding), FK506 Binding Protein Like, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
A variety of transcription factors and protein kinases involved in signal transduction are recovered from cells in heterocomplexes containing the abundant protein chaperone hsp90. Genetic studies in yeast have demonstrated that binding of steroid receptors, the dioxin receptor, and some protein kinases to hsp90 is critical for their signal transducing function in vivo. These heterocomplexes are formed by a multiprotein chaperone machinery consisting of at least four ubiquitous proteins--hsp90, hsp70, p60 and p23. Four high-molecular-weight immunophilins have been discovered as components of steroid receptor or other transcription factor complexes with hsp90. The immunophilins, protein chaperones with prolyl isomerase activity, bind the immunosuppressant drugs FK506 or CyP-40. These immunophilins all bind via tetratricopeptide repeat (TPR) domains to a single TPR binding site on each hsp90 dimer, and multiple heterocomplexes exist for each protein chaperoned by hsp90 according to the immunophilin ...
Redesigning an FKBP-ligand interface to generate chemical dimerizers with novel specificity. Proc Natl Acad Sci U S A (1998) 3.88 The immunophilin FK506-binding protein modulates Ca2+ release channel closure in rat heart. J Physiol (1997) 2.01 Inhibition of human immunodeficiency virus type 1 replication in human cells by Debio-025, a novel cyclophilin binding agent. Antimicrob Agents Chemother (2008) 1.46 Hair growth modulation by topical immunophilin ligands: induction of anagen, inhibition of massive catagen development, and relative protection from chemotherapy-induced alopecia. Am J Pathol (1997) 1.17 Rectification of skeletal muscle ryanodine receptor mediated by FK506 binding protein. Biophys J (1995) 1.10 A role for FKBP52 in Tau protein function. Proc Natl Acad Sci U S A (2010) 1.07 FKBP12 is a critical regulator of the heart rhythm and the cardiac voltage-gated sodium current in mice. Circ Res (2011) 0.99 From nature to the laboratory and into the clinic. Bioorg Med Chem (2008) 0.95 ...
Buy our Recombinant Human FKBPL protein. Ab119160 is a full length protein produced in Escherichia coli and has been validated in SDS-PAGE. Abcam provides free…
The FKBP39 from Drosophila melanogaster is a multifunctional regulatory immunophilin. It contains two globular domains linked by a highly charged disordered region. The N-terminal domain shows homology to the nucleoplasmin core domain, and the C-terminal domain is characteristic for the family of the FKBP immunophilin ligand binding domain. The specific partially disordered structure of the protein inspired us to investigate whether FKBP39 can drive spontaneous liquid-liquid phase separation (LLPS). Preliminary analyses using CatGranule and Pi-Pi contact predictors suggested a propensity for LLPS. Microscopy observations revealed that FKBP39 can self-concentrate to form liquid condensates. We also found that FKBP39 can lead to LLPS in the presence of RNA and peptides containing Arg-rich linear motifs derived from selected nuclear and nucleolar proteins. These heterotypic interactions have a stronger propensity for driving LLPS when compared to the interactions mediated by self-associating FKBP39 ...
FK 1012: consists of two FK-506 covalently bonded molecules; can bind two immunophilins at the same time thereby starting a part of the signal cascade that avtivates T-cells
Monoclonal antibody to Trigger Factor (TF) molecular chaperone can be used for western blotting or immunoprecipitation of TF-tagged recombinant proteins
Monoclonal antibody to Trigger Factor (TF) molecular chaperone can be used for western blotting or immunoprecipitation of TF-tagged recombinant proteins
Complete information for PPIL2 gene (Protein Coding), Peptidylprolyl Isomerase Like 2, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
It has been shown that rapamycin forms a complex with the immunophilin FK506-binding protein 12 (FKBP12), which then inhibits the protein kinase activity of mTOR ...
This is a replacement MIP X-DUTY Drive Hub, and is intended for use with the MIP X-DUTY CVD kit for the Traxxas Slash and Slash 4x4. This replacement... MIP10136
Looking for online definition of peptidylprolyl isomerase D in the Medical Dictionary? peptidylprolyl isomerase D explanation free. What is peptidylprolyl isomerase D? Meaning of peptidylprolyl isomerase D medical term. What does peptidylprolyl isomerase D mean?
The physiological roles of immunophilins are unclear, but many possess peptidyl-prolyl isomerase (PPIase) activity, and they have been found in all organisms examined to date, implying that they are involved in fundamental, protein-folding processes. The chloroplast thylakoid lumen of the higher plant Arabidopsis thaliana contains up to 16 immunophilins (five cyclophilins and 11 FKBPs), but only two of them, AtCYP20-2 and AtFKBP13, have been found to be active PPIases, indicating that the other immunophilins in this cellular compartment may have lost their putative PPIase activities. To assess this possibility, we characterized two independent Arabidopsis knockout lines lacking AtCYP20-2 in enzymological and quantitative proteomic analyses. The PPIase activity in thylakoid lumen preparations of both mutants was equal to that of corresponding wild-type preparations, and comparative two-dimensional difference gel electrophoresis analyses of the lumenal proteins of the mutants and wild type showed ...
Recombinant Peptidylprolyl Isomerase B (Cyclophilin B) (PPIB) Peptide. Species: Human. Source: Escherichia coli (E. coli). Order product ABIN934947.
In detail, tacrolimus reduces peptidylprolyl isomerase activity by binding to the immunophilin FKBP12 prograf , creating a new advanced. This FKBP12-FK506 complicated interacts with and inhibits calcineurin, thus inhibiting both T-lymphocyte sign transduction and IL-2 transcription. Within the previous 12 months, you may have heard about two new medications that had been approved by the FDA for use in kidney prograf transplant recipients, Nulojix® injection and Astagraf XL® (extended-release tacrolimus) capsules. Both of those medications have been accredited to be used along with steroids and mycophenolate to stop transplant rejection. The recommended initial dose of Prograf injection is 0.03-zero.05 mg/kg/day in kidney and liver transplant and zero.01 mg/kg/day in coronary heart transplant given as a continuous infusion into a vein . Cyclosporine is understood to trigger extra hair growth, and in some individuals this may be very outstanding. Buy prograf domestic. prograf Chronic Transplant ...
DROSOPHILA (ZOOLOGIE); GENREGULATION, REGULATION DER GENEXPRESSION (MOLEKULARBIOLOGIE); EPIGENETIK; TRANSKRIPTION (MOLEKULARE GENETIK); IMMUNOPHILINE (IMMUNOLOGIE); DROSOPHILA (ZOOLOGY); GENE REGULATION, REGULATION OF GENE-EXPRESSION (MOLECULAR BIOLOGY); EPIGENETICS; TRANSCRIPTION (MOLECULAR GENETICS); IMMUNOPHILINS (IMMUNOLOGY ...
Rotavirus (RV) causes more than 2 million diarrhea incidents and more than 600,000 deaths around the world every year. In order to prevent and treat this fatal disease, we must study, in depth, the mechanisms and pathogenesis of the individual viral proteins. It is known that RV non-structural protein 4 (NSP4) binds to several host-cell proteins, including cycophilin A and 40, which are immunophilins. In this study, we will specifically study another immunophilin/chaperone protein, Heat Shock Protein 56 (FKBP4). Previous studies suggest that FKBP4 plays an important role in transporting cholesterol from the ER to caveolae. During this study, we will use the yeast 2-hybrid assay (Y2H) to ascertain direct binding between NSP4 and FKBP4. Upon completion, other HSPs will be evaluated to gain a better understanding of the host cell interactions involved in pathogenesis of RV ...
ARZNEIMITTELENTWICKLUNG + ARZNEIMITTELDESIGN + ARZNEIMITTELENTDECKUNG; ORGANISCHE SYNTHESE (CHEMIE); PEPTIDYLPROLYL-ISOMERASE, CYCLOPHILIN (ENZYME); ENZYMINHIBITOREN (BIOCHEMIE); DRUG DEVELOPMENT + DRUG DESIGN + DRUG DISCOVERY (PHARMACY); ORGANIC SYNTHESIS (CHEMISTRY); PEPTIDYLPROLYL ISOMERASE, CYCLOPHILIN (ENZYMES); ENZYME INHIBITORS (BIOCHEMISTRY ...
PPIL1 antibody [N1C3] (peptidylprolyl isomerase (cyclophilin)-like 1) for ICC/IF, WB. Anti-PPIL1 pAb (GTX118126) is tested in Human samples. 100% Ab-Assurance.
1P5Q: 3D structure of human FK506-binding protein 52: Implications for the assembly of the glucocorticoid receptor/Hsp90/immunophilin heterocomplex
The protein encoded by this gene has similarity to the immunophilin protein family, which play a role in immunoregulation and basic cellular processes involving protein folding and trafficking. The encoded protein is thought to have a potential role in the induced radioresistance. Also it appears to have some involvement in the control of the cell cycle. [provided by RefSeq, Jul 2008 ...
SAFit2 is a highly potent, highly selective FK506-binding protein 51 (FKBP51) inhibitor with a Ki of 6 nM and also enhances AKT2-AS160 binding. - Mechanism of Action & Protocol.
FKBP52小鼠单克隆抗体[Hi52C](ab59460)可与小鼠, 大鼠, 仓鼠, 狗, 人样本反应并经WB, IP, IHC, Flow Cyt, ICC/IF实验严格验证。所有产品均提供质保服务,中国75%以上现货。
Staphylococcus aureus is a Gram-positive bacterium causing many kinds of infections from mild respiratory tract infections to life-threatening states as sepsis. It produces many toxins and has a remarkable ability to acquire resistance to antimicrobial drugs. Many S. aureus strains have acquired resistance to commonly used antibiotics and some strains are becoming multi-resistant. Methicillin-resistant strain of Staphylococcus aureus (MRSA) is the principal cause of severe nosocomial infections which can be fatal to compromised patients. Whole genome sequencing of two MRSA strains in 2001 was regarded as a way to find targets for novel antibiotics against infections caused by MRSA [1].. PrsA protein is found ubiquitously in Gram-positive bacteria, including S. aureus [Swiss-Prot:P60747], but not in Gram-negative ones [2, 3]. By sequence homology PrsA contains a parvulin-type peptidyl-prolyl cis-trans isomerase (PPIase) domain and flanking N- and C-terminal domains. PPIases are enzymes that ...
Use of FKBPL gene to identify a cause of infertility - Fertility problems affect (1 in 10) couples in Western society, making it one of the most common serious health issues. Despite this, little is known about the causes of infertility, and thus patient counseling and treatment are suboptimal. With infertility being such a common problem, identification of any cause would impact on a large number of patients, allowing better counseling, clearer diagnoses and the possibility of making more informed choices (e.g. adoption vs. IVF treatment). The present invention provides methods to identify a cause of infertility in a subject based on the genotype of the subject, in particular, by evaluating the status of the gene encoding FK506 binding protein-like (FKBPL). In particular, the present invention relates to use of the status of the gene encoding FK506 binding protein-like for identification of a cause of an infertile phenotype in a subject. Also provided, are methods method for identifying an ...
ProSpecs Cyclophilins include: Cyclophilin-A Human Recombinant, Cyclophilin-B Human Recombinant, Cyclophilin-D Human Recombinant
1Q1C: 3D structure of human FK506-binding protein 52: Implications for the assembly of the glucocorticoid receptor/Hsp90/immunophilin heterocomplex
Re: [dts] [PATCH V2]tests/shutdown_api: fix cant create vf 2020-03-20 8:37 [dts] [PATCH V2]tests/shutdown_api: fix cant create vf Zeng Xiaoxiao 2020-03-20 9:02 ` Zeng, XiaoxiaoX @ 2020-03-24 6:51 ` Tu, Lijuan 1 sibling, 0 replies; 4+ messages in thread From: Tu, Lijuan @ 2020-03-24 6:51 UTC (permalink / raw) To: Zeng, XiaoxiaoX, dts; +Cc: Zeng, XiaoxiaoX Applied, thanks , -----Original Message----- , From: dts [mailto:[email protected]] On Behalf Of Zeng Xiaoxiao , Sent: Friday, March 20, 2020 4:38 PM , To: [email protected] , Cc: Zeng, XiaoxiaoX ,[email protected], , Subject: [dts] [PATCH V2]tests/shutdown_api: fix cant create vf , , Signed-off-by: Zeng Xiaoxiao ,[email protected], , --- , tests/TestSuite_shutdown_api.py , 6 +++++- , 1 file changed, 5 insertions(+), 1 deletion(-) , , diff --git a/tests/TestSuite_shutdown_api.py , b/tests/TestSuite_shutdown_api.py index 11d451b..8699e79 100644 , --- a/tests/TestSuite_shutdown_api.py , +++ b/tests/TestSuite_shutdown_api.py , @@ ...
cytoplasm, nucleus, FK506 binding, peptidyl-prolyl cis-trans isomerase activity, protein dimerization activity, chaperone-mediated protein folding, protein folding
This report on the organ transplant immunosuppressant drugs market studies the current and future prospects of the global market.
The invention provides immunoassays for immunosuppressant drugs, wherein the assay is carried out under high salt conditions to achieve improved sensitivity. The invention also provides kits that are useful for performing the methods of the invention.
Immunosuppressive drugs ဆိုတဲ့ ေရာဂါျပီးစနစ္ကို သက္ေရာက္ေစတဲ့ ေဆးအမ်ိဳးအစားကလဲ ေက်ာက္ကပ္ကို ထိတယ္။ Glucocorticoids, Cytostatics, Antibodies, Drugs acting on immunophilins ေတြနဲ႔ တျခား ေဆးေတြ ျဖစ္တယ္။ ဒီေဆးမ်ိဳးေတြက ေက်ာက္ကပ္၊ ႏွလံုး၊ အသဲ၊ ရိုးတြင္းျခင္ဆီ လဲရသူေတြကို ေပးတာပါ။ ေနာက္ျပီး Rheumatoid arthritis, Multiple sclerosis, Myasthenia gravis, Systemic Lupus Erythematosus, Crohns disease, Pemphigus, Ulcerative colitis ဆိုတဲ့ေရာဂါေတြအတြက္ ေပးတယ္။ နာတာရွည္ အလာဂ်ီနဲ႔ ဆက္ေနတဲ့ ပန္းနာေရာဂါမွာလဲ သံုးတတ္တယ္ ...
Cyclophilin B, 0.1 ml. PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.
A validated positive silencing control targeting the Cyclophilin B (PPIB) gene in human, mouse, or rat cell lines. Useful for determination of optimal RNAi experimental conditions
PAE645Hu01, Polyclonal Antibody to FK506 Binding Protein 5 (FKBP5), P54; FKBP51; FKBP54; Ptg-10; Rotamase; 51 kDa FK506-binding protein; 54 kDa progesterone receptor-associated; Androgen-regulated protein 6; HSP90-binding immunophilin | Products for research use only!
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Principal Investigator:SUGAMURA Kazuo, Project Period (FY):1993 - 1995, Research Category:Grant-in-Aid for Developmental Scientific Research (B), Research Field:Immunology
Everolimus - Afinitor®/Votubia® - ist ein Derivat des natürlichen makrozyklischen Laktons Sirolimus mit immunsuppressiven und anti-angiogenen Eigenschaften. In Zellen bindet Everolimus an das Immunophilin FK-Bindungsprotein-12 (FKBP-12) und erzeugt damit einen immunsuppressiven Komplex, der die Aktivierung von mTOR (mammalian target of rapamycin) hemmt, eine Schlüssel-Kinase der Signalregulierung. Die Hemmung der mTOR Aktivierung führt zur Hemmung der T-Lymphozyten-Aktivierung und Proliferation, die wiederum mit der Antigen- und Zytokin-Stimulierung (IL-2, IL-4 und IL-15) sowie der Hemmung der Antikörperproduktion verbunden sind.. ...
Fkbp1b - Fkbp1b (Myc-DDK-tagged) - Mouse FK506 binding protein 1b (Fkbp1b) available for purchase from OriGene - Your Gene Company.
Shop Inactive peptidyl-prolyl cis-trans isomerase ELISA Kit, Recombinant Protein and Inactive peptidyl-prolyl cis-trans isomerase Antibody at MyBioSource. Custom ELISA Kit, Recombinant Protein and Antibody are available.
ppiase, cyp37, peptidyl-prolyl, cis-trans, isomerase, cyp37, chloroplastic, PPIase CYP37 | peptidyl-prolyl cis-trans isomerase CYP37, chloroplastic, P82869, ABF57273.1, AS10 1589
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One of the rate-limiting steps in protein folding has been shown to be the cis-trans isomerization of proline residues, which is catalyzed by a range of peptidylprolyl cis-trans isomerases. To characterize the interaction between model peptides and the periplasmic peptidylprolyl cis-trans isomerase SurA from E. coli, we employed a chemical cross-linking strategy that has been used previously to elucidate the interaction of substrates with other folding catalysts. The interaction between purified SurA and model peptides was significant in that it showed saturation and was abolished by denaturation of SurA; however the interaction was independent of the presence of proline residues in the model peptides. From results obtained by limited proteolysis we conclude that an N-terminal fragment of SurA, comprising 150 amino acids that do not contain the active sites involved in the peptidylprolyl cis-trans isomerization, is essential for the binding of peptides by SurA. This was confirmed by probing the ...
FAP48 was identified and cloned thanks to its interaction with FK506-binding proteins (FKBPs) such as FKBP52 and FKBP12, which belong to the large family of immunophilins that bind the macrolide immunosuppressant drugs FK506 and rapamycin. We have previously shown that FAP48-FKBP complexes are dissociated by FK506 and rapamycin, suggesting that FAP48 is an endogenous ligand of FKBP. The present work describes the biochemical consequences of FAP48 overexpression, induced by the tetracycline analogue doxycycline, in an established cell line derived from Jurkat T cells. We report that overexpression of FAP48 results in the inhibition of cellular proliferation as does the exposure of Jurkat T cells to FK506. We also show that the expression levels of argininosuccinate synthetase and the Myc antagonist Mxi1 are modified by overexpression of FAP48, suggesting that these proteins could be good candidates to mediate the antiproliferative effect of FAP48. FAP48 affects neither the calcineurin-dependent ...
The protein encoded by this gene is a member of the immunophilin protein family, which play a role in immunoregulation and basic cellular processes involving protein folding and trafficking. This encoded protein is a cis-trans prolyl isomerase that binds the immunosuppressants FK506 and rapamycin. It is thought to function as an ER chaperone and may also act as a component of membrane cytoskeletal scaffolds. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Sep 2008 ...
eng] Cyclophilin-D (CyP-D) is a peptidyl prolyl cis/trans isomerase located in the mitochondrial matrix of mammalian cells. The subcellular localization of the protein is determined by the presence of a mitochondrial targeting presequence. In the first part of this work, we characterized human CyP-D presequence allowing the protein translocation into mitochondria. We showed that the 16 first amino acid of the presequence are necessary and sufficient to form a functional presequence and to address hCyP-D into mitochondria. One of the main physiological roles of CyP-D is to activate the mitochondrial permeability transition pore (mPTP) opening. The mPTP is a protein complex formed during oxidative stress and leading to cell necrosis. Thus, CyP-D may be considered as a necrosis inductor. Nevertheless, several studies have also shown that CyP-D exhibits a protective role toward apoptosis induced by oxidative stress. However, the mechanism implicated in the cellular protection conferred by CyP-D is ...
Legionella pneumophilia is a gram-negative rod which is able to multiply within phagocytic cells. The process of phagocytosis leads to a rapid environmental change that might require a coordinate regulation of gene expression to ensure intracellular survival. Since there is little information on up- and downregulation of genes during the early phases of phagocytosis, we radiolabeled intracellular L. pneumophila at different times after phagocytosis by macrophages of the Mono Mac 6 cell line and immunoprecipitated antigens with antilegionella sera or monoclonal antibodies. We could identify two antigens which were upregulated, one of which was the Mip protein, three antigens which were downregulated, and three antigens which were not detectable in extracellularly grown L. pneumophila. The Mip protein was stained most intensively 4 to 8 h after intracellular infection, suggesting that it is needed during intracellular multiplication rather than initiation of infection. A 44-kDa antigen which was ...
PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides (By similarity).