Citation. Finak G, Langweiler M, Jaimes M, Malek M, Taghiyar J, Korin Y, Raddassi K, Devine L, Obermoser G, Pekalski ML, Pontikos N, Diaz A, Heck S, Villanova F, Terrazzini N, Kern F, Qian Y, Stanton R, Wang K, Brandes A, Ramey J, Aghaeepour N, Mosmann T, Scheuermann RH, Reed E, Palucka K, Pascual V, Blomberg BB, Nestle F, Nussenblatt RB, Brinkman RR, Gottardo R, Maecker H, McCoy JP. Standardizing Flow Cytometry Immunophenotyping Analysis from the Human ImmunoPhenotyping Consortium.. Scientific Reports. 2016 Feb 10; 6: 20686.. External Citation. Abstract. Standardization of immunophenotyping requires careful attention to reagents, sample handling, instrument setup, and data analysis, and is essential for successful cross-study and cross-center comparison of data. Experts developed five standardized, eight-color panels for identification of major immune cell subsets in peripheral blood. These were produced as pre-configured, lyophilized, reagents in 96-well plates. We present the results of a ...
Flow cytometry immunophenotyping is invaluable for the diagnostic and prognostic work-up of haematological malignancies. Over the last decade, multi-colour flow cytometry analysis has been used increasingly to investigate leptomeningeal disease; several studies show the utility and sensitivity of this technique for both B cell Non-Hodgkins Lymphoma and Acute Leukaemia diagnostics. Cells within CSF samples are typically low in number and degrade quickly. Therefore, the recommendation is to use cell stabilisation reagents for CSF samples that require flow cytometry analysis. Previous studies have shown that TransFix stabilised CSF samples have a cell yield equal to or better than that of fresh CSF samples and so may be used for immunophenotypic analysis after 18 hours of storage. Some cell characteristics may change after TransFix treatment: for example, light scatter properties of peripheral blood and CSF leukocytes may be altered and the fluorochrome signal intensity of certain bound antisera ...
LifeLabs is excited to introduce an expansion of our flow cytometry testing. Starting on April 10th, 2017 we will offer flow cytometric immunophenotyping for hematopoietic and lymphoid malignancies in addition to our existing flow cytometry testing for T-cell subset analysis.. Flow cytometry is widely used for analyzing the expression of surface and intracellular molecules in order to differentiate and characterize different cell populations. It continues to be a necessary diagnostic tool for the classification, staging, and monitoring of hematolymphoid neoplasms.. LifeLabs is pleased to offer a variety of panels to facilitate the diagnosis and/or prognosis of the following:. ...
The BD OneFlow™ ALOT (Acute Leukemia Orientation Tube) is a pre-configured single-dose, ready-to-use 8-color reagent. Two tubes, one containing the cytoplasmic markers (C tube) and one containing the surface markers (S tube), provide a single-test.. The BD OneFlow ALOT is intended for flow cytometric immunophenotyping of aberrant immature populations of hematopoietic cells (lymphoid and non-lymphoid lineage) in bone marrow and peripheral blood as an aid in the diagnosis of acute lymphoblastic leukemia and non-lymphoid acute leukemia.. As a screening tube, the BD OneFlow ALOT can guide the need for further analysis in combination with panel(s) specifically designed for the classification of different forms of B, T and myeloid acute leukemias.. The BD OneFlow ALOT is available in the 10 test/box size (4 pouches of 5 tubes each: 2 pouches of S tubes and 2 pouches of C tubes).. Dark red color-coded boxes, pouches and tubes allow for easy visual identification.. The reagent composition is shown ...
EuroFlow™ is a trademark of the EuroFlow Consortium. Unless otherwise noted, for In Vitro Diagnostic Use. CE marked to the European In Vitro Diagnostic Medical Device Directive 98/79/EC.. Not available for sale in the US.. 1 J.J.M. van Dongen, L. Lhermitte, S. Böttcher, et al on behalf of the EuroFlow Consortium (EU-FP6, LSHB-CT-2006-018708). EuroFlow antibody panels for standardized n-dimensional flow cytometric immunophenotyping of normal, reactive and malignant leukocytes. Leukemia 2012, 26 (9): 1908-75.. ...
Antibody reagents that are used for flow cytometry immunophenotyping have traditionally been prepared by combining individual liquid antibody conjugates into mixtures. These cocktails have limited...
Immunophenotyping is the analysis of heterogeneous populations of cells for the purpose of identifying the presence and proportions of the various populations of interest. Clusters of Differentiation (CD) antigens are widely used for immunophenotyping.
Chattopadhyay, P. K. and Roederer, M. 2005. Immunophenotyping of T Cell Subpopulations in HIV Disease. Current Protocols in Immunology. 65:12.12:12.12.1-12.12.15. ...
ABSTRACT Immunophenotyping of paucicellular samples may represent a diagnostic challenge in the flow cytometry (FC) laboratory routine, as the scarcity of cells limits the number of tests that can be performed
I just wondered: 1 Can I access any demo versions of immunophenotyping software thru www or similar? BD, Verity, DAKO ?? (Mac or PC) 2 Any other newsgroup discussing flow cytometry? www-servers? Thanks ...
Immunophenotyping. Immunophenotyping holds immense potential in the field of drug discovery. Reverse engineering, the idea that we can discover the biological or technological principles of something by studying its external structure and operation, allows scientists to develop new drug targets.In other words, by studying the exterior surface cell markers, one can determine the internal structure of the cell, perhaps even at the genomic and DNA level. [13,16,17] The process of drug delivery starts with the identification of appropriate drug targets. Only then can suitable drugs be developed to specifically and effectively target diseases or problems.This step is greatly aided by immunophenotyping in which the many clusters of differentiation are cataloged according to their presence in a sample. Once the targets, the surface proteins and carbohydrates, are identified, suitable drugs can then be developed to work on these specific molecules. [13,16,17]. For example, new drug targets are being ...
1. Doyle HA, Yan J, Liang B, Mamula MJ. Lupus autoantigens: their origins, forms, and presentation. Immunol. Res. 2001; 24; 131-147.. 2. Tan EM. Autoantibodies to nuclear antigens: Their immunobiology and medicine. Adv Imm. 1982; 33: 167-239.. 3. Wakeland EK, Liu K, Graham RR, Behrens TW. Delineating the genetic basis of systemic lupus erythematosus. Immunity. 2001, 15: 397-408.. 4. Gaffney PM, Moser KL, Graham RR, Behrens TW. Recent advances in the genetics of systemic lupus erythematosus. Rheum Dis Clin North Am 2002, 28: 111-126.. 5. Okumura N, Nomura M, Tada T. Effects of sample storage on serum C3 assay by immunonephelometry. Clin Lab Sci. 1990; 3; 54-57.. 6. Cohen M. Systemic Lupus Erythematosus: diagnosis and classification. Intern Med J. 2004; 34(12): 701-702.. 7. Deng YJ, Huang ZX, Zhou CJ, Wang JW, You Y, Song ZQ, Xiang MM, Zhong BY, Hao F. Gene profiling involved in immature CD4+ T lymphocyte responsible for systemic lupus erythematosus. Molecular Immunology. 2006; 43: ...
Every tube / specimen is a patient with a suspected illness in need of a diagnosis and appropriate course of treatment. Without our adept analytical and interpretive skills, the clinical physician would be at a loss in providing the appropriate care and course of treatment. It is even more critical as in my case where youre one of only two skilled personnel in respect to certain diagnostic procedures (Flow Cytometry for the immunophenotyping of leukemias ...
Patients with uncharacteristic inflammatory symptoms such as long-standing fatigue or pain, or a prolonged fever, constitute a diagnostic and therapeutic challenge. The aim of the present study was to determine if an extended immunophenotyping of lymphocytes and monocytes including activation markers can define disease-specific patterns, and thus provide valuable diagnostic information for these patients. Whole blood from patients with gram-negative bacteraemia, neuroborreliosis, tuberculosis, acute mononucleosis, influenza or a mixed connective tissue disorders, as diagnosed by routine culture and serology techniques was analysed for lymphocyte and monocyte cell surface markers using a no-wash, no-lyse protocol for multi-colour flow cytometry method. The immunophenotyping included the activation markers HLA-DR and CD40. Plasma levels of soluble TNF alpha receptors were analysed by ELISA. An informative pattern was obtained by combining two of the analysed parameters: (i), the fractions of HLA-DR
Background: Peripheral blood involvement is recognised as an adverse prognostic factor in Mycosis Fungoides (MF) & Sézary Syndrome (SS) which is reflected in the revised staging for MF/SS using blood (B) classification. Various methods exist to assess B classification.Objective: To assess peripheral blood involvement in MF/SS using T-cell receptor (TCR) analysis, lactate dehydrogenase levels and flow cytometric immunophenotyping. Methods: 57 consecutive patients with MF/SS assessed at our Cutaneous Lymphoma Centre, University Hospital Birmingham, UK between 2011 and 2014 were identified: 30 with early stage (I-IIA) and 27 with advanced disease (IIB-IV).Results: In early stage disease, 2/30 (7%) had identical TCR clones in skin and blood compared to 14/27 (52%) in advanced disease (p | 0.001). Abnormal immunophenotyping was found in 7/27 patients (26%) with advanced disease (p = 0.003) which included 6/14 (43%) erythrodermic patients but none in early stage disease. Elevated LDH was the most frequent
Background: Despite the diagnostic utility of immunophenotyping for myelodysplastic syndromes (MDS), it has not been widely performed, and reports on this are absent in Korea. We aimed to evaluate the immunophenotypic features of non-blastic granulocytes, monocytes, and blasts in patients with MDS and non-clonal disorders using routine flow cytometry (FCM). Moreover, we evaluated the phenotypic abnormalities of mature cells in leukemic patients. Methods: Marrow aspirates from 60 patients, including 18 with MDS, 18 with leukemia, and 24 with non-clonal disorders (control group), were analyzed using FCM. Blasts, non-blast myeloid cells, and monocytes were gated based on CD45 expression and side scatter (SSC). The phenotypes were then compared among the 3 groups. Results: Compared to non-clonal disorders, the granulocytic lineages of MDS showed decreased SSC (P=0.005), increased CD45 intensity (P=0.020), decreased CD10-positive granulocytes (P= 0.030), and a higher CD56-positive rate (P=0.005). It ...
FC allows correlation of up to six different markers on a single cell and is much faster than immunohistochemistry (IHC), which can only analyze one marker per slide. In addition, FC can be used on peripheral blood, bone marrow aspirate, and cerebrospinal fluid - samples that are not able to be analyzed by IHC. Overall, FC is less expensive and much faster (one to two lab hours) to process than IHC, although the interpretation by a skilled technician remains.. The one disadvantage of FC is that the architecture of a sample is lost as the cells are analyzed in a suspension, making this method less desirable than tumor sections or slides in solid tumors where the architecture of the tumor is important in staging.. Because this new technology detects very low levels of B-cell leukemic cells, it will enable clinicians to cease therapy with a level of clinical certainty unheard of in the recent past, thus potentially preventing unnecessary courses of expensive therapy. Common current therapy includes ...
The SMA-negative cells in all three cases showed distinct negativity to ER and CK8, in sharp contrast with the overlying epithelial cells that showed strong ER and CK8 positivities (data not shown). The distribution of these SMA-negative ME cells seemed to be independent of the ductal size, length, and architecture.. Our findings are consistent with those of a recent study showing that a vast majority of the ME cells in both normal and ductal carcinoma in situ displayed distinct immunostaining to p63, SM-MHC, and calponin, whereas a single or a cluster of a few ME cells in some ducts failed to show immunoreactivity to all these three markers [28]. However, our study differs from this study [28] and previous studies [3, 4] in four aspects: first, the SMA-negative cells were segmented, accounting for at least one-third or all of the ME cells in involved ducts; second, we tested for more ME cell markers; third, the SMA-negative cells assessed were morphologically similar to their adjacent ...
This unit on basic phenotyping describes two basic and two alternate protocols for the immunophenotypic identification and classification of human peripheral blood lymphocytes
Purpose: Classification of acute leukemia (AL) is based on commitment of leukemic cells to the myeloid or the lymphoid lineage. However, a small percentage of AL cases lack straightforward immunophenotypical lineage commitment. These leukemias of ambiguous lineage represent a heterogeneous category of AL that cannot be classified as either myeloid AL (AML) or lymphoid AL (ALL). The lack of clear classification of acute leukemias of ambiguous lineage as either AML or ALL is a hurdle in treatment choice for these patients. Experimental design: here, we compared the microRNA expression profiles of 17 cases with AL of ambiguous lineage and 16 cases of AML, B-ALL and T-ALL Results: We show that leukemias of ambiguous lineage do not segregate as a separate entity but exhibit microRNA expression profiles similar to either AML, B-ALL or T-ALL. We show that by using only five of the most lineage discriminative microRNAs we are able to define AL of ambiguous lineage as either AML or ALL. Conclusions: Our ...
The Invitrogen™ eBioscience™ Super Bright polymer dyes represent a suite of bright fluorophores excited by the violet laser (405 nm). Optimized for use in flow cytometry, the Super Bright dyes allow for expanded use of violet laser excitation and promote streamlined multicolor panel design. This scientific poster describes the use of these dyes and the Invitrogen ™ Attune™ NxT Flow Cytometer to &
Data accumulated to date indicate that HIV-infected cells persist for prolonged periods and undergo clonal expansion; it remains uncertain to what degree clonally expanded populations contribute to the population of replication-competent cells, which represent the relevant reservoir that requires eradication or long-term control. In an exhaustive analysis of 213 uninduced proviruses, Ho and colleagues found that only 11.3% were full length (21). It is not known whether any of these proviruses were members of a clonally expanded population, but the data indicate that few integrants, expanded or not, are replication competent. Cohn and colleagues (17) analyzed 75 proviruses from expanded populations and did not identify any full-length HIV proviruses, suggesting the frequency of clonally expanded populations with replication-competent virus is low and may not contribute significantly to the HIV reservoir.. In contrast to the findings described above, direct demonstration of replication-competent ...
Definition : Reagents used in cytologic assays performed by flow cytometers. They include labels for cell membranes and standards to normalize instrument setup, cell count, and proliferation. These reagents are frequently available in kits that allow the calibration, control, and standardization of flow cytometry analysis.. Entry Terms : "Antibodies, Monoclonal, Flow Cytometry" , "Flow Cytometry Reagents" , "Reagents, Lymphoma Cell Immunophenotype" , "Reagents, Lymphocyte T/B Cell Subpopulation" , "Reagents, Lymphocyte T/B Cell Population" , "Reagents, Lymphocyte T Cell Population" , "Reagents, Flow Cytometry" , "Reagents, Cytology/Histology, Flow Cytometry". UMDC code : 17052 ...
Boer OJ, Loos CM van der, Hamerlinck F, Bos JD, Das PK, Reappraisal of in situ immunophenotypic analysis of psoriasis skin: interaction of activated HLA-DR+ immunocompetent cells and endothelial cells is a major feature of psoriatic lesions. Arch Dermatol Res 1994; 286: 87-96 ...
In Project 2, you will progress through the normal sequence of events in forward (classical) genetics. Forward genetics investigations work to tie a gene to its function. This type of work generally starts with finding a worm with an aberrant phenotype (mutant) that is likely to be caused by a defect in a protein encoded by a mutated gene. In our investigation we will work to first locate a mutated gene in such a mutant in order to characterize the exact change in the gene (mutation) responsible for the aberrant phenotype. Working with abnormal genes/proteins facilitates understanding of the importance of the gene, gene product or functionally significant gene region in C. elegans and in other species. When we are able to make these structure/function connections by studying mutant worms and identifying the genes responsible for the defects, the main goal is not so much to understand defective gene function in worms, but rather, to be able to extrapolate the function of normal genes by seeing ...
In Project 2, you will progress through the normal sequence of events in forward (classical) genetics. Forward genetics investigations work to tie a gene to its function. This type of work generally starts with finding a worm with an aberrant phenotype (mutant) that is likely to be caused by a defect in a protein encoded by a mutated gene. In our investigation we will work to first locate a mutated gene in such a mutant in order to characterize the exact change in the gene (mutation) responsible for the aberrant phenotype. Working with abnormal genes/proteins facilitates understanding of the importance of the gene, gene product or functionally significant gene region in C. elegans and in other species. When we are able to make these structure/function connections by studying mutant worms and identifying the genes responsible for the defects, the main goal is not so much to understand defective gene function in worms, but rather, to be able to extrapolate the function of normal genes by seeing ...
Immunodeficiency Profile II - Flow Immune Deficiency Profile II - T and B-cell Panel - T, B and NK-cell Panel - CD3 and T-Cell Subsets - CD19 and NK Cell Panel - CD4/CD8 - Immunophenotyping - CD3 - CD4 - CD8 - Lymphocyte Quantitation ( T, B and NK) ...
We offer a comprehensive line of positive procedural controls and cellular stabilization products for immunophenotyping by flow cytometry.
Topic: Immunophenotyping - from the Bench to the Clinic. Minimum eligibility: The participant must be a MD or PhD and working in a clinical cytometry laboratory. The candidates will be selected based on their bio-data.. Number of participants will be restricted to only 4 Venues: ...
research, immunological basis of reversible and fixed airways, glóbulo blanco linfocito t visto con una micrografía, 活细胞 好搜百科, immunophenotyping in bk virus allograft nephropathy
Fine needle aspiration cytology and flow cytometry immunophenotyping of non-Hodgkin lymphoma: can we do better?. Objective: To evaluate the diagnostic efficiency of fine needle aspiration cytology/flow cytometry (FNAC/FC) in the diagnosis and classification of non-Hodgkin lymphoma (NHL) in a series of 446 cases and to compare the results with those of previous experiences to evaluate whether there had been an improvement in FNAC/FC diagnostic accuracy.. Methods: FNAC/FC was used to analyse 446 cases of benign reactive hyperplasia (BRH), NHL and NHL relapse (rNHL) in 362 lymph nodes and 84 extranodal lesions. When a diagnosis of NHL was reached, a classification was attempted combining FC data and cytological features. Sensitivity, specificity and positive and negative predictive values (PPV and NPV) of FNAC/FC in the diagnosis and classification of NHL were calculated and compared with those available in the literature.. Results: FNAC/FC provided a diagnosis of NHL and rNHL in 245 cases and of ...
Dogs spontaneously develop B-cell chronic lymphocytic leukemia (CLL), providing a naturally occurring model to study genetic risk factors and new therapeutics. CLL accounts for approximately 10% of canine lymphoma and leukemia samples submitted for flow cytometric immunophenotyping at the Colorado State University Clinical Immunology laboratory. There is a strong breed predilection in canine CLL, suggesting genetic predisposition (Bromberek et al, 2016). Our goals are to investigate the molecular mechanisms and clinical features of canine CLL, to evaluate this disease as a model for human CLL.. We previously investigated immunoglobulin heavy chain variable region (IGHV) mutation status in canine CLL patients and found breed-specific differences in mutation status. The majority of small-breed dogs, which have a strong predilection for CLL, had mutated IGHV genes (M-CLL), while Boxer dogs preferentially had unmutated IGHV genes (U-CLL)(79% of cases). We investigated the clinical presentation and ...
Lymphomas are a heterogeneous group of neoplasias originating from B- or T-lymphocytes. In this thesis, we determined the genetic and immunophenotypic characterization of DLBCL and their prognostic impact. Moreover, genomic alterations associated with the transformation to DLBCL from Hodgkin lymphoma (HL) and follicular lymphoma (FL) were elucidated. In order to outline the impact of cytogenetic as well as immunophenotypic prognostic markers in DLBCL, we firstly studied a series of 54 DLBCL tumors using comparative genomic hybridization (CGH) and we identified several frequently occurring chromosomal imbalances. Loss of 22q was more often found in the diagnostic tumors with a more advanced clinical stage, while gain of 18q21 was more commonly identified in relapses. Secondly, we correlated the expression patterns of CD10, bcl-6, IRF-4 and bcl-2 with clinical parameters in a series of 173 de novo DLBCL patients. Patients with a germinal center (GC) phenotype displayed a better survival than the ...
Microglia and macrophages play a central role in neuroinflammation. Pro-inflammatory cytokines trigger their conversion to a classically activated (M1) phenotype, sustaining inflammation and producing a cytotoxic environment. Conversely, anti-inflammatory cytokines polarize the cells towards an alternatively activated (M2), tissue reparative phenotype. Elucidation of the signal transduction pathways involved in M1 to M2 phenotypic conversion may provide insight into how the innate immune response can be harnessed during distinct phases of disease or injury to mediate neuroprotection and neurorepair. Microglial cells (cell line and primary) were subjected to combined cyclic adenosine monophosphate (cyclic AMP) and IL-4, or either alone, in the presence of pro-inflammatory mediators, lipopolysaccharide (LPS), or tumor necrosis factor-α (TNF-α). Their effects on the expression of characteristic markers for M1 and M2 microglia were assessed. Similarly, the M1 and M2 phenotypes of microglia and macrophages
In a recent unit in this series, protocols for the isolation, cryopreservation, thawing, and immunophenotyping of HPBMC isolated from peripheral whole blood using cell surface marker (CSM) staining and multi‐color flow cytometry analysis were presented
MRD in AML can be based on molecular methods (PCR targeting fusion genes, mutations or expression levels of appropriate genes) or on multicolour flow cytometry. In flow cytometric MRD analysis leukemia-associated immunophenotypes (LAIPs) are indentified at diagnosis and applied to follow-up samples. Earlier, with four to six colour combinations, patient-specific LAIPs were designed on the basis of antigen profiles at diagnosis. Our strategy with eight to ten colour flow cytometry is to apply four standard combinations (for granulocytes, monocytes, stem cells and aberrant markers, respectively). This strategy not only allows the identification of LAIPs but also the analysis of granulocytic and monocytic differentiation patterns. The combinations also contain markers to identify e.g. mast cells, plasmacytoid dendritic cells, basophils and plasma cells, which may contaminate the so called blast area in CD45/side scatter plots. We in Tampere have three-year experience of ten-colour flow cytometric ...
Background Multiparameter flow cytometric analysis of bone marrow and peripheral blood cells has ... syndromes . However, the usefulness of flow cytometry for the detection of megakaryocytic and platelet .... Research Article last updated 06/15/2012 - 2:25pm.. ...
www.MOLUNA.de Flow Cytometry in Hematopathology [4221757] - Although instrumentation and laboratory techniques for flow cytometry (FCM) immunophenotyping of hematopoietic malignancies are well documented, there is relatively little information on how best to perform data analysis, a critical step in FCM testing. In Flow Cytometry in Hematopathology: A Visual Approach to Data Analysis and Interpretation, three physicians highly
Four-color Eye-makeup - Applying an eye makeup design could be the answer to your beauty problems. Learn more about applying an eye makeup design from HowStuffWorks.
Novelty: Unbiased analysis of immunophenotyping of blood from a cohort of moderate, severe, recovered COVID-19 donors and healthy control reveals selective clustering of severe individuals. Severe cases have oligoclonal expansion of antibodies, which have long CDR3s, indicating multi-reactivity, compared to moderate cases that had a more diverse clonal expansion. The increased frequencies of neutrophil and eosinophil populations were not associated with an activated phenotype. They highlight a decrease of CD15 expression in neutrophils and CD16 expression in different innate immune cell populations such as NK cells that could be interesting to investigate further. Standing in the field: They show extensive data on blood immune cell composition between different subgroups of COVID-19 patients that supports other studies, which they always cite. Appropriate statistics: The statistical tools used are very clear and all listed in the method section of the paper. Viral model used: SARS-CoV2 from ...
... develops world-class, cutting-edge antibodies and reagents for biomedical research to accelerate research and discovery by providing the highest quality products at an outstanding value.. Biolegends product areas include cell immunophenotyping for Flow cytometry and Fluorescent Microsope, Cytokines&Chemokines, Adhesion, Cancer research, T regulatory cells, Stem cells, Innate immunity, Cell-cycle analysis, Apoptosis, and modification-specific antibodies.. Advanced Medical Science is an authorized Exclusive Distributor of Bilolegend in Thailand. ...
(2012) Zhu et al. PLoS ONE. Dendritic cells (DCs) regulate innate and acquired immunity through their roles as antigen-presenting cells. Specific subsets of mature DCs, including monocyte-derived and lymphoid-derived DCs, can be distinguished based on distinct immunophenotypes and functional prop...
... : Assayed controls for monitoring immunophenotyping by flow cyt
Flow cytometric analysis of expression of surface markers on DCs with different treatments.(A) Phenotypic changes in expression of CD40, CD80 and CD86 maturatio
Soma Technology inc. - Offers Used and Refurbished GE Dash 4000 Multiparameter Monitors Featuring Modular Flexibility, and NIBP Accuracy
TY - JOUR. T1 - The prognostic value of multiparametric flow cytometry in AL amyloidosis at diagnosis and at the end of first-line treatment. AU - Muchtar, Eli. AU - Jevremovic, Dragan. AU - Dispenzieri, Angela. AU - Dingli, David M. AU - Buadi, Francis K.. AU - Lacy, Martha. AU - Gonsalves, Wilson. AU - Hayman, Suzanne R.. AU - Kapoor, Prashant. AU - Leung, Nelson. AU - Russell, Stephen J. AU - Lust, John A.. AU - Lin, Yi. AU - Go, Ronald S.. AU - Chakraborty, Rajshekhar. AU - Zeldenrust, Steven. AU - Kumar, Shaji K. AU - Kyle, Robert A.. AU - Rajkumar, S Vincent. AU - Gertz, Morie. PY - 2017/1/5. Y1 - 2017/1/5. N2 - Multiparametric flow cytometry (MFC) in amyloid light-chain (AL) amyloidosis has not been widely adopted and, consequently, there is little information on its clinical relevance. We studied 173 patients with AL amyloidosis who underwent MFC immunophenotyping of bone marrow sample at diagnosis and 82 patients at the end of the first line of treatment (EOT). The number of monotypic ...
Minimal residual disease evaluation refers to a series of molecular and immunophenotypical techniques aimed at detecting submicroscopic disease after therapy. As such, its application in acute myeloid leukemia has greatly increased our ability to quantify treatment response, and to determine the chemosensitivity of the disease, as the final product of the drug schedule, dose intensity, biodistribution, and the pharmakogenetic profile of the patient. There is now consistent evidence for the prognostic power of minimal residual disease evaluation in acute myeloid leukemia, which is complementary to the baseline prognostic assessment of the disease. The focus for its use is therefore shifting to individualize treatment based on a deeper evaluation of chemosensitivity and residual tumor burden. In this review, we will summarize the results of the major clinical studies evaluating minimal residual disease in acute myeloid leukemia in adults in recent years and address the technical and practical issues still
This is a page with useful consensus documents and standards for people interested in clinical flow cytometry. The standards are listed in alphabetical order . If we are missing something, please feel free to contribute by adding it here. 2006 Bethesda International Consensus Recommendations on Immunophenotypic Analysis of Hematolymphoid Neoplasia by Flow Cytometry. Cytometry Part B, 2007. 2006 Bethesda International Consensus Recommendations on the Immunophenotypic Analysis of Hematolymphoid Neoplasia by Flow Cytometry: Recommendations for Training and Education to Perform Clinical Flow Cytometry, Cytometry Part B 2007 Clinical Flow Cytometric Analysis of Hematolymphoid Cells; Approved Guideline - Second Edition H43-A2 Clinical and Laboratory Standards Institute (CLSI) 2007 Enumeration of Immunologically Defined Cell Populations by Flow Cytometry; Approved Guideline-Second Edition H42-A2 Clinical and Laboratory Standards Institute (CLSI) 2007. MMWR 2003: Guidelines for Performing ...
Kardum Paro, Mirjana Mariana and Šiftar, Zoran and Kardum-Skelin, Ika and Šušterčić, Dunja and Nazor, Aida and Flegar-Meštrić, Zlata and Jakšić, Branimir (2010) Flow cytometry immunophenotyping (FCI) of fine needle aspirates (FNAs) of lymph nodes. Collegium Antropologicum, 34 (2). pp. 359-65. ISSN 0350-6134 Kardum-Skelin, Ika and Jelić Puškarić, Biljana and Radić-Krišto, Delfa and Jakšić, Ozren and Kardum, Matko and Jakšić, Branimir (2010) Multimodal image analysis of chronic leukemic lymphoproliferative disorders and the hypothesis of "single" and "multiple" programmed stops in the development of typical and atypical forms of leukemias and lymphomas. Collegium Antropologicum, 34 (2). pp. 367-76. ISSN 0350-6134 Jelić-Puškarić, Biljana and Ostojić-Kolonić, Slobodanka and Planinc-Peraica, Ana and Obad-Kovačević, Dragica and Kardum-Skelin, Ika and Jakšić, Branimir (2010) Myeloid sarcoma involving the breast. Collegium Antropologicum, 34 (2). pp. 641-4. ISSN 0350-6134 ...
Common Variable Immune Deficiency (CVID) is a syndrome containing a spectrum of disorders which results in weakened immunity and recurrent infections. The ESID (European Society for Immunodeficiencies) CVID definition includes patients with marked decrease of IgG (at least 2 standard deviations below the mean for age). Patients must also have disease onset at an age over 2 years, absent isohaemagglutinins and/or response to vaccines and other defined causes of hypogammaglobulinaemia must be excluded. The Euroclass system of classifying CVID is the result of a European multicentre trial attempting to develop a consensus of two existing classification schemes of B-cell immunophenotyping. In this paper it was shown that B-cell immunophenotype correlated with coincidence of clinical sequelae and it suggested implementing this to further classify CVID to give prognostic and therapeutic information. However, it has not yet been shown that these alterations in B-cell immunophenotype are the result of ...