Caulfield, M J.; Proffitt, M R.; and Cerny, J, Induction of idiotype-specific suppressor cells (sc) with antigen/antibody complexes. Abstr. (1982). Subject Strain Bibliography 1982. 1718 ...
Two synergizing antigen-specific helper T (Th) cell populations are required for an optimal TEPC15 (T15)-dominated antiphosphorylcholine (PC) plaque- forming cell response . In these studies, the two Th cell sets are shown to differ in their requirements for recognition of self-major histocompatibility complex (MHC)-encoded determinants by testing the ability of Th cells from F(1) {arrow} parent bone marrow chimeras to collaborate with PC-specific B cells bearing MHC-encoded determinants of either parental haplotypes. Previous studies have shown that one antigen-specific Th cell population is required for T-dependent anti-PC responses and activates PC-specific B cells only if the hapten, PC, is physically linked to the priming antigen. This Th cell, referred to as ThMHC, induces anti-PC responses that are mainly non-T15 in character, and it appears to be identical to the conventional antigen- specific Th cell. In these experiments, using T cells from (A X B)F(1) {arrow} parent A chimeras, ThMHC ...
Ossendorf V, Cornely O, Draube A, Monsef I, Engert A, Skoetz N. Idiotype vaccination for Non-Hodgkin lymphoma. Cochrane Database of Systematic Reviews 2016, Issue 2. Art. No.: CD008964. DOI: 10.1002/14651858.CD008964. ...
Rubin, B; Hertel, wulff B.; and Kimura, A, Alloantigen-specific idiotype-bearing receptors on mouse t lymphocytes. I. Specificity characterization and genetic association with the heavy-chain igg allotype. (1979). Subject Strain Bibliography 1979. 4576 ...
Antigen-binding receptors on T lymphocytes and IgG antibodies with the same antigen-binding specificity as the T-cell receptors display shared or identical idiotypes. This was shown using a system where adult F1 hybrid rats between two inbred strains were inoculated with T lymphocytes from one parental strain. Such F1 hybrid rats produce antibodies directed against idiotypic determinants present on IgG alloantibodies, produced in the T donor genotype strain and with specificity for the alloantigens of the other parental strain. The idiotypic nature of the F1 antialloantibody serum against the parental alloantibodies was demonstrated both by indirect hemagglutination tests or by gel diffusion using alloantisera with different specificity as targets. Furthermore, the F1 anti-T-lymphocyte sera could be shown to contain antibodies against idiotypic parental T lymphocytes as well. This was shown by the capacity of the antisera, in the presence of complement, to wipe out the relevant parental T-cell ...
Dive into the research topics of A common idiotype expressed on a murine anti-Sm monoclonal antibody and antibodies in SLE sera. Together they form a unique fingerprint. ...
Recently the minor B cell subpopulation that expresses the CD5 (Leu-1) antigen has been implicated as a source of IgM autoantibodies. Chronic lymphocytic leukemia (CLL), the most common leukemia in humans, represents a malignancy of small B lymphocytes that also express the CD5 antigen. However, little is known concerning the antibody variable region genes (V genes) that are used by these malignant CD5 B cells. We have found that a relatively high frequency of CLL patients have leukemic B cells with surface immunoglobulin (sIg) recognized by 17.109, a murine mAb specific for a kappa light chain associated crossreactive idiotype (CRI) associated with rheumatoid factor and other IgM autoantibodies. Flow cytometric analyses revealed that the relative expression of the 17.109-CRI by circulating leukemic B cells was directly proportional to the levels of sIg kappa light chain, indicating that there exists stable idiotype expression in the leukemic population. To examine this at the molecular level, ...
Looking for Idiotypes? Find out information about Idiotypes. The unique amino acid sequence and corresponding three-dimensional structure of the variable region of an immunoglobulin molecule that determines its... Explanation of Idiotypes
Rabbit anti-idiotypic antibodies were prepared by injection of specifically purified anti-p-azobenzoate antibodies (D) from individual donor rabbits. Benzoate derivatives were found to be strong inhibitors of the reactions of D with anti-D antisera. There was a close correlation between the combining affinities of the benzoate derivatives used and their effectiveness as inhibitors. Compounds tested that are chemically unrelated to benzoate were ineffective. The results indicate either that the combining site of anti-benzoate antibody is part of an important idiotypic determinant, which is sterically blocked by hapten, or that the hapten induces a conformational change which alters idiotypic determinants not involving the active site. Such conformational changes, if they occur, must be restricted since hapten has little effect on the reactions of F(ab)2 fragments of anti-benzoate antibodies with antisera directed to rabbit fragment Fab and no detectable effect on reactions with antibodies ...
Author(s): Markus Brede ,Ulrich Behn Subject(s): CX.3 Category: Abstract:. The talk deals with modelling a subsystem of the immune system, the so-called idiotypic network. Idiotypic networks, a concept conceived by N.K. Jerne in 1974, are functional networks of interacting antibodies and B-cells. In principle, Jernes framework provides solutions to many issues in immunology, such as immunological memory, mechanisms for antigen recognition and the question of self/non-self discrimination. Explaining the interconnection between the elementary components local dynamics, network formation and architecture, and possible modes of global system function appears to be an ideal playground of statistical mechanics. We present a simple cellular automaton model based on a graph representation of the system. From a simplified description of idiotypic interactions rules for the random evolution of networks of occupied and empty sites on these graphs are derived. In certain biologically relevant parameter ...
The anti-idiotypic (anti-Id) antibody (Ab) 9G4 binds a cross-reactive idiotope (CRI) present in a select group of human autoantibodies. This Id has been localized to the portion of immunoglobulin (Ig) heavy (H) chains encoded by the VH4-21 gene segment, a member of the human VH4 family. This gene segment is utilized by essentially all cold agglutinin (CA) Abs with I/i specificity isolated from patients with CA disease stemming from chronic lymphoproliferative disorders. In this study, mutational analysis of a CA has been used to determine the structural basis for 9G4 binding to Abs utilizing the VH4-21 gene segment. Recombinant CA H chain mutants were produced and their 9G4 reactivity determined. Mutants were generated by exchanging VH4-21 sequences in the FR1, CDR1, and CDR2 with corresponding sequences from a closely related gene segment V71-2, a VH4 family member that is associated neither with Abs having CA activity nor with Abs that react with 9G4. The results indicate that the motif AVY at ...
Purchase Idiotypes in Medicine: Autoimmunity, Infection and Cancer - 1st Edition. Print Book & E-Book. ISBN 9780444828071, 9780080534435
A monoclonal anti-idiotypic antibody specific to a human IgG 1 type monoclonal antibody possessing specificity to nicotinic acetylcholine receptor; a method for the production of the aforementioned monoclonal anti-idiotypic antibody by the steps of immunizing an animal with a human IgG 1 type monoclonal antibody specific to nicotinic acetylcholine receptor, collecting antibody-producing cells from the animal, fusing the collected cells with neoplastic cells, selecting from the product of fusion a hybridoma capable of producing a monoclonal anti-idiotypic antibody specific to the human IgG 1 type monoclonal antibody possessing specificity to nicotinic acetylcholine receptor, propagating the selected hybridoma thereby giving rise to said monoclonal anti-idiotypic antibody, and collecting the produced monoclonal anti-idiotypic antibody; and use of the monoclonal anti-idiotypic antibody as a reagent and as an adsorbent.
A monoclonal anti-idiotypic antibody specific to a human IgG1 type monoclonal antibody possessing specificity to nicotinic acetylcholine receptor; a method for the production of the aforementioned monoclonal anti-idiotypic antibody by the steps of immunizing an animal with a human IgG1 type monoclonal antibody specific to nicotinic acetylcholine receptor, collecting antibody-producing cells from the animal, fusing the collected cells with neoplastic cells, selecting from the product of fusion a hybridoma capable of producing a monoclonal anti-idiotypic antibody specific to the human IgG1 type monoclonal antibody possessing specificity to nicotinic acetylcholine receptor, propagating the selected hybridoma thereby giving rise to said monoclonal anti-idiotypic antibody, and collecting the produced monoclonal anti-idiotypic antibody; and use of the monoclonal anti-idiotypic antibody as a reagent and as an adsorbent.
Hybridoma-derived idiotype vaccines have been used for the experimental treatment of human lymphoma over the last twenty years, providing evidence of biological efficacy, clinical efficacy and clinical benefit. However, the product that has come closer to regulatory approval is unlikely to clear that hurdle due to the insufficiently robust data obtained in a recently closed clinical trial. This review aims at discussing the reasons for hybridoma-derived idiotype vaccines, more difficult to produce but also more successful than recombinant idiotype vaccines so far, are unlikely to gain regulatory approval. In particular, it is necessary to examine the many peculiar features of this therapeutic approach in a broader context, with special attention to concepts like customized active immunotherapy and randomization. Most published trials based on hybridoma-derived idiotype vaccines are being analyzed, together with the yet non-peer reviewed data from the only randomized study conducted so far with this
OBJECTIVE: To investigate whether autoreactive mechanisms occur in Lyme disease (LD) by determining IgA, IgG and IgM rheumatoid factor (RF) concentrations and RF associated cross reactive idiotype (CRI) expression in the serum of LD patients, with comparison to patients with rheumatoid arthritis (RA). METHODS: The RF isotype profiles were determined in 59 patients with LD; erythema migrans (EM) (n=19), neuroborreliosis (NB) (n=20) and Lyme arthritis (LA) (n=20). Mouse monoclonal antibodies (mAbs) G6 and G8 (V(H)1 gene associated), D12 (V(H)3 gene associated) and C7 (V(kappa)III gene associated) were then used to determine the RF associated CRI expression on IgM antibodies in 16 of these LD patients (eight seropositive for RF); (EM (n=3), NB (n=6), LA (n=7)). RESULTS: Seven (18%) patients with either NB or LA had increased concentrations of IgA RF compared with none with EM. Significant differences in the number of patients with raised concentrations of IgG RF or IgM RF were not found between the LD
High-dose therapy followed by autologous transplantation has improved survival in MM, however all patients eventually relapse, indicating the need for consolidation. Activation of idiotype-specific T cells by vaccination with autologous tumorprotein-loaded dendritic cells has been demonstrated. So-called Vaccibodies (consisting of two scFv (single chain Fragment variable) specific for surface molecules on APC (MHCII, CD40) linked to two exchangeable scFv from the mouse M315 myeloma protein and a Cγ3 dimerizing domain) elicit anti-Id antibodies in mice even in the absence of adjuvant, and stimulate Id-specific CD4+ T cells in vitro ...
Cell surface receptors for the 86-kDa glycoprotein (gp86) of human cytomegalovirus (HCMV) were identified by using two monoclonal anti-idiotype antibodies that bear the internal image of gp86. These antibodies bound to cells permissive for HCMV infection by both ELISA and immunofluorescence assay and inhibited HCMV plaque formation in human embryonic lung (HEL) cells. Immunoblot analysis showed specific binding of both internal image anti-idiotype antibodies as well as gp86 to an HEL cell membrane protein with an approximate molecular mass of 92.5 kDa. In addition, immunoprecipitation of radiolabeled membrane and cell surface proteins from human foreskin tissue, human foreskin fibroblasts, or HEL cells showed specific binding of anti-idiotype antibody predominantly to the 92.5-kDa protein.. ...
Many monoclonal antibodies that react with the lacto-N-fucopentaose III (LNF III) antigenic determinant, Gal beta 1-4(Fuc alpha 1-3)GlcNAc beta 1-3Gal beta 1-4Glc, have been described recently. The terminal trisaccharide of this determinant, fucosyllactosamine, is present on glycolipids and glycoproteins and on the surface of granulocytes, monocytes, and other cells. To study the structural and genetic diversity of these antibodies, syngeneic anti-idiotypic monoclonal antibodies were produced in BALB/c mice against PMN 6, a monoclonal antibody directed against this sequence. Anti-idiotypic antibodies 6B1 and 6C4 reacted with 50% of a panel of 20 anti-LNF III monoclonal antibodies, whereas 6A3 reacted strongly only with PMN 6. This indicates that the determinants recognized by 6C4 and 6B1 represent major cross-reactive idiotopes of this family of antibodies. The binding of idiotypic antibodies to a glycolipid bearing this antigenic determinant was completely inhibited by the three anti-idiotypic ...
Immunogenicity of Protein-315 and its Fab fragment (Fab-315) for isologous and heterologous strains of mice was investigated by comparing the characteristics of the anti-idiotypic response produced in BALB/c and A/J mice. The ability of these anti-idiotypic antisera to compete with DNP-lys for the ligand-binding site of Protein-315 were analyzed by comparing their hapten inhibition curves.. The anti-idiotypic responses of BALB/c mice to Protein-315 and to Fab-315 were similar to one another with regard to antibody specificity and sensitivity to inhibition by hapten, suggesting that both forms were equally immunogenic in inbred BALB/c mice. This observation indicates that the Fc portion of Protein-315 is not essential for the induction of anti-idiotypic response. Both BALB/c and A/J mice recognized the same antigenic determinants on Fab-315 since the anti-idiotypic antibodies produced by these animals were indistinguishable with regard to their interaction with Protein-315, Fv-315, and ...
B-cell lymphomas express surface immunoglobulin (immunoglobulin) containing unique idiotypic (idiotype) determinants which may be exploited as tumor specific markers. The inventor has produced murine monoclonal antibodies (MAbs) reactive with the idiotype marker derived from 67 patients with low grade, follicular, small cleaved cell lymphoma. Out of 199 monoclonal antibodies, 47 (24%) were found to react with pooled normal human serum immunoglobulin in concentrations ranging from 0.6 μg/ml to 160 μg/ml. Of these 40 monoclonal antibodies, 90% cross-reacted with idiotype present in normal serum in levels |50 μg/ml. Thirty-two of these anti-idiotypes were directed against a shared idiotope expressed on another patients lymphoma cells. The frequency of shared idiotope expression defined by each antibody ranged from 0.26% to 3.9% of the B-cell lymphomas tested. A panel of five anti-idiotype antibodies reacted with 80% of AIDS associated lymphomas. Based on the reactivity with these monoclonal antibodies,
Cardiac Myosin and Autoimmune Myocarditis (N. Rose et al.). Intracellular Autoantigens: Diagnostic Fingerprints but Aetiological Dilemmas (E. Tan et al.).. Significance of Carbohydrate Components of Cell Surfaces (T. Feizi).. How is Tolerance Generated (G. Nossal).. Therapeutic Immune Regulation in Experimental Interstitial Nephritis with Suppressor T-Cells and Their Soluble Factors (C. Kelly et al.).. Regulation of HLA Class II Expression and Its Role in Autoimmune Disease (M. Feldmann).. Idiotypes and Autoimmunity (J. Kearney et al.).. Molecular Basis for the Cross-reactive Idiotypes on Human Anti-IgG Autoantibodies (D. Carson et al.).. Autoimmunity and Immunodeficiency Disease (F. Rosen).. Deficiency of the Effector Mechanisms of the Immune Response and Autoimmunity (P. Lachmann & M. Walport).. Monoclonal Anti-Ia Antibody Therapy in Animal Models of Autoimmune Disease (H. McDevitt et al.).. Index of Contributors.. Subject Index.. ...
A panel of idiotypically cross-reactive murine monoclonal antibodies (Mabs) isolated in response to fluorescein (Fl) was generated and segregated based on Fl affinity. Intermediate affinity prototype Mab 9-40 (IgG1, $\kappa$; K$\sb{\rm a}$ = 3.3 $\times$ 10$\sp7$M$\sp{-1}$) possessed $>$90% active site quaternary structural homology to the intermediate affinity 9-40 idiotype family (comprised of 12-40, 3-24, 10-25, 5-14 and 5-27). The 9-40 family was 40-100% and 10-40% idiotypically related to high affinity prototype Mab 4-4-20 (IgG2a, $\kappa$; K$\sb{\rm a}$ = 1.8 $\times$ 10$\sp{10}$M$\sp{-1}$) and low affinity prototype Mabs 3-13 and 3-17 (K$\sb{\rm a}$ $\sim$ 5.0 $\times$ 10$\sp4$M$\sp{-1}$), respectively. Mabs 4-4-20 and 3-13/3-17 were idiotypically distinct. This anti-Fl panel spanned a greater affinity range than any previously reported idiotype family and was exploited to define specific active site residues (and their interactions with antigen) responsible for the observed Fl binding ...
TY - JOUR. T1 - The physiology of anti-idiotypic interactions. T2 - From clonal to paratopic selection. AU - Greally, John M.. PY - 1991. Y1 - 1991. N2 - On theoretical and experimental grounds, it has been proposed that the idiotypes of immunoglobulins and of T cell receptors are composed of multiple paratopes, as opposed to a single paratope and several idiotopes. This necessitates a revision of some of the basic principles of anti-idiotypic reactions. It is also possible to infer the presence of the same or similar paratopes on different idiotypes. A paratope cannot therefore be regarded as restricted to or unique on an idiotype. For these reasons, the perception of immunological specificity in terms of clonal units is misleading. This review proposes instead that the physiological unit of immunological specificity and regulation is the paratope. This essential alteration in the perception of the immune system is referred to as paratopic selection. The approach is assessed in terms of ...
Specific Interference with the Determination of the Tumour-Associated Glycoprotein 72 by Human Anti-Idiotypic Antibodies Formed after Treatment with the Anti-Tumour-Associated Glycoprotein 72 Antibody ...
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Three IgM monoclonal anti-DNA antibodies were produced by hybridoma techniques from an MRL-lpr/lpr mouse using denatured DNA (dDNA) as the selection antigen. All three antibodies also bound poly(dT), poly(rA), and the single-stranded random copolymer poly(dI,dT), and each antibody displayed a unique preference for a limited array of other ribo- and deoxyribopolynucleotides based on direct binding as well as inhibition studies. Inability to identify a common primary structure in the polynucleotides reactive with each antibody suggested that higher ordered structures may be important. This notion was supported by the finding that oligomers of thymidine of 25-30 nucleotides or less were ineffective in blocking antibody binding to dDNA or poly(dT). However, deliberate destabilization of putative secondary structures by decreasing counterion concentration and increasing temperature had little effect on antibody binding to poly(dT). Since the antigenic polynucleotides in general contain little known ...
An Idiotypic sequence of the light chain of the myeloma protein M315 produced by the MOPC315 tumor has been defined. This sequence is presented on MHC class II molecules to Id-specific CD4+ T cells. T cell receptor (TCR) genes from this clone were isolated and used to establish a TCR-transgenic mouse. Such TCR-transgenic mice are resistant to challenge with MOPC315 cells and the resistance can be transferred to other mice with purified Id-specific CD4+ T cells. Further experiments have demonstrated that myeloma protein secreted by the s.c. myeloma prime dendritic cells in the tumor with Id. These Id-primed dendritic cells then stimulate CD4 Id-specific CD4+ T cells that as activated cells somehow kill the myeloma cells.. In parallel, in vitro models have established mature B cells from pro-B cells and further generated membrane immunoglobulin B cells or plasma cells by additional stimuli , which may be improved by lymph node dendritic cells as well as antigen selected as idiotype specific ...
Anti-idiotype vaccination represents an innovative approach to target tumor-associated antigen-expressing cells. This approach comes directly from Jernes idiotypic network theory, which postulates that due to the huge potentiality for diversity of the immunoglobulin variable regions, the idiotype repertoire can mimic the universe of self and foreign epitopes [1].. NeuGc-containing gangliosides are attractive targets for cancer immunotherapy because these glycolipids are non-self antigens in humans [2, 3]. In contrast, they have been detected in different human tumors by antibodies and chemical analysis [4-6]. Recent experimental data suggest that N-glycolyl-GM3 ganglioside (NeuGcGM3) is relevant for tumor biology [7].. mAb-1E10 [8] is an IgG1 anti-idiotype (Ab2) mAb obtained by immunizing Balb/c mice with mAb-P3 (Ab1) [9] coupled to keyhole limpet hemocyanin (KLH) in the presence of Freunds adjuvant. This Ab2 inhibited the binding of mAb-P3 to NeuGcGM3 ganglioside. mAb-1E10 induced an ...
TY - JOUR. T1 - Emergence of immunoglobulin variants following treatment of a B cell leukemia with an immunotoxin composed of antiidiotypic antibody and saporin. AU - Glennie, M. J.. AU - McBride, H. M.. AU - Stirpe, F.. AU - Thorpe, P. E.. AU - Worth, A. T.. AU - Stevenson, G. T.. PY - 1987. Y1 - 1987. N2 - The potency and specificity of immunotoxins consisting of monoclonal antiidiotype conjugated to the ribosome-inactivating protein, saporin, have been evaluated in the treatment of guinea pig L2C B lymphocytic leukemia. The immunotoxins were therapeutically much more effective than their parent antibodies. Their specificity reflected that of their antiidiotype component. Although the leukemia emerged eventually in most animals treated with these conjugates, most of the cells showed altered Ig expression, which rendered them resistant to the therapy. Commonly, the emerging cells had lost μ heavy chain production, leaving them negative for intracellular, surface, and secreted IgM, but still ...
IDIOTYPES according to the free Medical Dictionary. The unique and characteristic parts of an antibodys variable region, which can themselves serve as antigens.
BACKGROUND: Therapeutic idiotypic (Id) vaccination is an experimental treatment for selected B cell malignancies. A broader use of Id-based vaccinatio
References for Abcams Anti-Progesterone Receptor (phospho S162) antibody [1064/E2] (ab58564). Please let us know if you have used this product in your…
E. S. Aleksandrova, F. Koralewski, M. I. Titov, A. V. Demin, A. N. Ignatova, A. V. Kozyr, A. V. Kolesnikov, A. Tramontano, S. Paul, D. Thomas, A. G. Gabibov, A. Friboulet ...
A major challenge in vaccinology is to predict vaccine efficacy [31-33]. Here, we used a multiparametric systems biology approach to identify gene signatures predictive of an immune response, using an experimental platform based on PBMCs from 6 HCV-positive subjects stimulated ex vivo with the IGKV3-20 light-chain protein, as candidate idiotype vaccine.. The cytokine pattern induced by IGKV3-20 was assessed by ELISA in culture supernatant of stimulated PBMCs, after 24 h or 6 days of incubation (Figure 1).. The results show that the stimulation induces an overall significant production of both Th1 (TNF-α) and Th2 cytokines (IL-6 and IL-10), with a prevalence of the latters. However, specific samples consistently show very different levels of TNF-α and IL-6 production, which are highest for samples BE and DN and lowest for sample MML. These results, although based on a small cohort, indicate a significant difference in the individual response to the same antigen and, in particular, suggest that ...
Polyclonal antibody for NR3C3/PGR detection. Host: Rabbit.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Human. NR3C3/PGR information: Molecular Weight: 98981 MW; Subcellular Localization: Nucleus. Cytoplasm. Nucleoplasmic shuttling is bo
Rabbit Polyclonal ID4 antibody N-Term for WB. Published in 2 Pubmed References. Order this anti-ID4 antibody. | Product number ABIN2780374
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Our laboratory is developing cancer vaccines that are being evaluated in animal models for their protective activity against tumors before they are administered to cancer patients. The vaccines are composed of antibodies mimicking tumor antigens (anti-idiotypic antibodies) or the antigens expressed in viruses (recombinant adeno- or vaccinia-viruses) and are selected based on their high probability of inducing both humoral and cellular immune responses in patients. The animal models we have developed for preclinical evaluation of the vaccines closely mimic the condition in cancer patients. In other studies which have reached clinical trials, we are evaluating the vaccinated patients immune responses to their tumors ...
This paper evaluates a representative sample of the best anti-ID and pro-ID publications and presents a conclusion as to the present state of the evidence and arguments regarding these positions. Published in Origins, n. 63.
Although there is multiple evidence for the functional heterogeneity and polyclonality of TRAb (1 - 3) both statements are questioned by some investigators (4, 5). Furthermore there is growing...
Η δημιουργία κι ο εμπλουτισμός της Πλατφόρμας τεκμηρίωσης των ερευνητικών αποτελεσμάτων και των ερευνητών (CRIS) Πανδώρα, έγινε στο πλαίσιο του Υποέργου 4 «Προμήθειες Εξοπλισμού Λογισμικού» της πράξης «Ψηφιακές υπηρεσίες ανοιχτής πρόσβασης της βιβλιοθήκης του Πανεπιστημίου Πειραιώς» με κωδικό ΟΠΣ «304169», του Επιχειρησιακού Προγράμματος Ψηφιακή Σύγκλιση ...
Η δημιουργία κι ο εμπλουτισμός της Πλατφόρμας τεκμηρίωσης των ερευνητικών αποτελεσμάτων και των ερευνητών (CRIS) Πανδώρα, έγινε στο πλαίσιο του Υποέργου 4 «Προμήθειες Εξοπλισμού Λογισμικού» της πράξης «Ψηφιακές υπηρεσίες ανοιχτής πρόσβασης της βιβλιοθήκης του Πανεπιστημίου Πειραιώς» με κωδικό ΟΠΣ «304169», του Επιχειρησιακού Προγράμματος Ψηφιακή Σύγκλιση ...
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Neuroblastoma treatment with chimeric anti-disialoganglioside GD2 antibody ch14.18 showed objective anti-tumor responses. Production of anti-idiotypic antibodies (Ab2) against ch14.18 (Ab1) in some cases was positively correlated with a more favorable prognosis. According to Jernes network theory, a subset of anti-idiotypic antibodies (Ab2beta) form an internal image of the antigen and induce antibodies (Ab3) against the original antigen. The molecular origin of the anti-idiotypic antibody response in tumor patients was not investigated previously. To clone anti-idiotypic antibodies, B-cells of a ch14.18-treated neuroblastoma patient with Ab2 serum reactivity were used to construct antibody phage display libraries (Methods described in Arthritis Rheum. 2000, 43:2722-2732). Upon repetitive selection of lambda and kappa Fab-phage display libraries on target antigens ch14.18 and the murine equivalent 14G2a, positive binders were enriched. Selected Ab2-clones GK2 and GK8 as well as another 38 ...
Eight monoclonal antibodies from the primary response of C57BL/6 mice against the hapten (4-hydroxy-3-nitrophenyl)acetyl (NP) were isolated. The antibodies carry lambda 1 light chains and have similar affinities for the immunizing hapten. Sequence analysis at the level of mRNA reveals that all antib …
General It is not known whether Simulect use will have a long-term effect on the ability of the immune system to respond to antigens first encountered during Simulect -induced immunosuppression. Immunogenicity Of renal transplantation patients treated with Simulect and tested for anti-idiotype antibodies, 4/339 developed an anti-idiotype antibody response, with no deleterious clinical effect upon the patient. In none of these cases was there evidence that the presence of anti-idiotype antibody accelerated Simulect clearance or decreased the period of receptor saturation. In Study 2, the incidence of human anti-murine antibody (HAMA) in renal transplantation patients treated with Simulect was 2/138 in patients not exposed to muromonab-CD3 and 4/34 in patients who subsequently received muromonab-CD3. The available clinical data on the use of muromonab-CD3 in patients previously treated with Simulect suggest that subsequent use of muromonab-CD3 or other murine anti-lymphocytic antibody preparations ...
Understanding the molecular mechanisms of immunological memory assumes importance in vaccine design. We had earlier hypothesized a mechanism for the maintenance of immunological memory through the operation of a network of idiotypic and anti-idiotypic antibodies (Ab2). Peptides derived from an internal image carrying anti-idiotypic antibody are hypothesized to facilitate the perpetuation of antigen specific T cell memory through similarity in peptide-MHC binding as that of the antigenic peptide. In the present work, the existence of such peptidomimics of the antigen in the Ab2 variable region and their similarity of MHC-I binding was examined by bioinformatics approaches. The analysis employing three known viral antigens and one tumor-associated antigen shows that peptidomimics from Ab2 variable regions have structurally similar MHC-I binding patterns as compared to antigenic peptides, indicating a structural basis for memory perpetuation. (C)) 2007 Elsevier Inc. All rights reserved.. ...
Therapy of lymphomas with immunotoxins comprised of soluble T-cell receptors conjugated to ricin A-chain.. Immunotoxins are conjugates of cell-binding moieties linked to toxins or toxin subunits. Many investigators have used antibodies directed against tumor specific-antigens as the cell- binding moiety. B-cell tumors are ideal for this type of therapy because of their tumor-specific idiotype (Id). However, B-cell tumors often secrete Id which competes with tumor cells for binding of anti-Id antibodies. Therefore, to overcome this obstacle, my research focuses on developing soluble T-cell receptors (TCR) from tumor specific T cell clones as the cell-binding moiety, and ricin-A chain as the toxic moiety. T-cells recognize cell-bound (but not soluble) antigen in association with products of the major histocompatibility complex. Therefore, free idiotype should not interfere with binding of soluble TCRs to tumor cells. Id specific T-cells have been cloned from mice immunized with the murine tumor, ...
Progesterone Receptor antibody [C262], C-term (progesterone receptor) for IP, WB. Anti-Progesterone Receptor mAb (GTX80350) is tested in Human, Mouse, Rat, Rabbit samples. 100% Ab-Assurance.
Antibodies, Treatment, Human, Patients, Plasma, Serum, Igg, Immunoglobulin, Monoclonal Antibodies, Therapeutic, Anti-idiotype Antibodies, Breast, Breast Cancer, Calibration, Cancer, Elisa, Enzyme-linked Immunosorbent Assay, Horseradish, Horseradish Peroxidase, Immunoassay
T cell vaccination (TCV) activates Tregs of 2 kinds: anti-idiotypic (anti-id) and anti-ergotypic (anti-erg). These regulators furnish a useful view of the physiology of T cell regulation of the immune response. Anti-id Tregs recognize specific effector clones by their unique TCR CDR3 peptides; anti-id networks of CD4+ and CD8+ Tregs have been described in detail. Here we shall focus on anti-erg T regulators. Anti-erg T cells, unlike anti-id T cells, do not recognize the clonal identity of effector T cells; rather, anti-erg T cells recognize the state of activation of target effector T cells, irrespective of their TCR specificity. We consider several features of anti-erg T cells: their ontogeny, subset markers, and target ergotope molecules; mechanisms by which they regulate other T cells; mechanisms by which they get regulated; and therapeutic prospects for anti-erg upregulation and downregulation.. ...
The basic components of the diagnostic test systems are antigens and specific antibodies. The main objective of developing express tests for the diagnosis of bovine leukemia virus (BLV) is to obtain a virus antigen drug, which is very time-consuming to prepare. This problem can be solved by producing anti-idiotype antibodies that have a chemical structure identical to that of the viral antigen and does not require large expenditures to manufacture [1, 2 ...
Rabbit recombinant monoclonal Progesterone Receptor antibody [SP2] validated for IHC and tested in Human. Immunogen corresponding to recombinant full length…
|strong|Human anti Cetuximab, clone AbD19834_IgG1|/strong|, is an anti-idiotypic antibody that binds specifically to cetuximab and inhibits the binding of the drug to its target, epidermal growth fact…
|strong|Human anti golimumab, clone AbD20692_hIgG1|/strong| is a Type 1 anti-idiotypic antibody that specifically recognizes free golimumab but not the drug/tumor necrosis factor alpha (TNFα) co…
To everyone who may not have heard the news our dear friend, wife, mother, sister, and daughter Lorena Ra… Jojo Cris Isaiah needs your support for Loris Fund
Matusevich, O V; Egorov, V V; Gluzdikov, I A; Titov, M I; Shtro, A A; Slita, A V; Dukov, M I; Shurygina, A-P S; Smirnova, T D; Kudryavtsev, I V; Vasin, A V; Kiselev, O I; Zarubaev, Vladimir V. ...
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成會明。2004。奈米碳管。五南出版社。 韋進全、張先鋒、王坤林著、楊明勳校訂。2009。奈米碳管巨觀體。五南出版社。 國立科學工藝博物館網站 http://nano.nstm.gov.tw。 黃建盛。2006。奈米碳管簡介。科學新天地第十三期。 黃啟仁。2008。哺乳動物及家禽免疫細胞作為益生菌調控免疫機制於體外模式之探討。碩士論文。中興大學。台中。 葉晨聖、曾 厚、張佑民、何佳安、黃世宏、李明威、陳志宏、方偉峰、孫啟光、王國禎、張立惠、黃暄益、吳信毅、陳東煌、吳炳慶、徐瑋玲、李國賓、黃志嘉、鄭豐裕、陳昭瑜、盧陽明、張富雄、廖韋晴、楊宗翰、陳敏慧、楊智强、謝達斌、楊鏡堂、蘇家豪、蕭俊龍。2007。教育部「生物及醫學科技人才培育先導型計畫」生醫奈米技術。 劉吉平、郝向陽。2003。奈米科學與技術。世茂出版社。 ...
Solé-Medina, Aida; Heer, Katrin; Opgenoorth, Lars; Kaldewey, Phillip; Danusevicius, Darius; Notivol, Eduardo; Robledo-Arnuncio, Juan José; Ramírez-Valiente, José Alberto ...
diff --git a/bfd/ChangeLog b/bfd/ChangeLog index cefe44a..4ce81a6 100644 --- a/bfd/ChangeLog +++ b/bfd/ChangeLog @@ -1,3 +1,104 @@ +2016-05-28 Alan Modra ,[email protected], + + * aoutx.h: Adjust linker callback calls throughout file, + removing dead code. + * bout.c: Likewise. + * coff-alpha.c: Likewise. + * coff-arm.c: Likewise. + * coff-h8300.c: Likewise. + * coff-h8500.c: Likewise. + * coff-i960.c: Likewise. + * coff-mcore.c: Likewise. + * coff-mips.c: Likewise. + * coff-ppc.c: Likewise. + * coff-rs6000.c: Likewise. + * coff-sh.c: Likewise. + * coff-tic80.c: Likewise. + * coff-w65.c: Likewise. + * coff-z80.c: Likewise. + * coff-z8k.c: Likewise. + * coff64-rs6000.c: Likewise. + * cofflink.c: Likewise. + * ecoff.c: Likewise. + * elf-bfd.h: Likewise. + * elf-m10200.c: Likewise. + * elf-m10300.c: Likewise. + * elf32-arc.c: Likewise. + * elf32-arm.c: Likewise. + * elf32-avr.c: Likewise. + * elf32-bfin.c: Likewise. + * elf32-cr16.c: Likewise. + * elf32-cr16c.c: Likewise. + * elf32-cris.c: Likewise. ...