Author Summary Each time a mammalian cell duplicates its genome in preparation for cell division it activates thousands of so called
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Constant region of immunoglobulin heavy chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens (PubMed:22158414, PubMed:20176268). The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for
Antibodies are produced by plasma cells, the terminally differentiated descendants of the B-cell lineage. The early stages of B-cell development occur in the bone marrow, where pro-B cells, which are derived from hematopoietic stem cells, undergo rearrangement of the immunoglobulin heavy-chain genes. This process occurs throughout the life of the individual. At the next stage of B-cell differentiation, in pre-B cells, there is rearrangement of the light-chain genes. This allows the expression of the intact immunoglobulin molecule on the cell surface of the B cell. Each B cell and its progeny express only one rearranged immunoglobulin heavy chain and one light chain. When antigen binds to the surface of the B cell, if the appropriate environmental signals are received, the B cell proliferates and differentiates into a memory B cell that can respond more rapidly to future exposures to that antigen or to a plasma cell that secretes high concentrations of antibodies.. ...
We report the sequence of a cDNA encoding a rabbit immunoglobulin γ heavy chain of d12 and e14 allotypes with high homology to… Expand ...
Details on the datasets generation: 169 structures of protein antigens (length >30 amino acids) in complex with antibody fragments have been manually collected from the PDB of January 2006 at a resolution ≤ 4 Å. Every structure has been manually curated. Structures in which the antibody binds antigen but involves no CDR residues have been excluded from the analysis; there were four such structures [PDB: 1MHH, 1HEZ, 1DEE, 1IGC]. If a structure contained several complexes in one asymmetric unit (there were 46 such structures in 165) and the authors of the structure observed no structural difference between these complexes, only one complex was selected - those that were specified as a reference complex by the authors of the article describing the structure (primary citation in the PDB); there were 18 such structures out of 46. If the authors didnt provide this information, all complexes in the structure were considered for analysis. The authors of a few structures clearly stated in their ...
Puri, J; Ben, neriah Y.; Givol, D; and Lonai, P, "Antibodies to immunoglobulin heavy chain variable regions protect helper cells from specific suicide by radiolabeled antigen." (1980). Subject Strain Bibliography 1980. 1413 ...
In Burkitt lymphoma the c-myc gene, the cellular homologue of the viral oncogene v-myc, has been implicated in the aetiology of this human B-cell malignancy. Burkitt lymphoma cells possess specific chromosomal rearrangements involving the region proximal to the c-myc gene and one of the three human immunoglobulin loci. The nature of the effect exerted by the immunoglobulin loci on the translocated c-myc gene is controversial: whereas some reports have suggested c-myc transcription is elevated in Burkitt lymphoma cells, others have suggested the level of transcription is unaffected by the translation. Recently, transcription enhancer elements have been identified in the intron between the JH and C mu segments of the heavy-chain immunoglobulin gene in mice. If similar enhancers exist in humans they may lead to increased transcription of the translocated c-myc gene and thus contribute to oncogenesis in Burkitt lymphoma. We report here the identification of an enhancer element adjacent to the human C mu
Faust, C H.; Moore, J M.; and Heim, I E., "Mouse immunoglobulin mu heavy chain mrna of y5781, a high yield myeloma." (1979). Subject Strain Bibliography 1979. 640 ...
Depending on whether the nature of the antigen is protein or polysaccharide and the frequency and duration of stimulation by the antigen, an antibody response may exhibit changes in the distribution of IgG subclasses in plasma, and cause increased or diminished levels of one or more IgG subclasses (Meulenbroek et al., 2000). "When the serum level of a subclass is below detection levels of the most sensitive techniques (ELISA/RIA), it is considered as a complete deficiency /absence or a total lack" (Meulenbroek et al., 2000). Such complete deficiency is rare and is usually due to deletions in chromosome 14 loci. "Such a total lack of one or more IgG subclasses due to deletions of the immunoglobulin heavy chain constant region genes is occasionally found in healthy individuals. The fact that these individuals still produce protective antibody titers in the residual immunoglobulin classes or subclasses suggests that the deletion of the isotype(s) occurs by chance and can be compensated adequately" ...
TY - JOUR. T1 - NF-HB (BSAP) is a repressor of the murine immunoglobulin heavy-chain 3′α enhancer at early stages of B-cell differentiation. AU - Singh, Mallika. AU - Birshtein, Barbara K.. PY - 1993/6. Y1 - 1993/6. N2 - We have identified a nuclear factor expressed in pro-B-, pre-B-, and B-cell lines that binds to two sites within the murine immunoglobulin heavy-chain (IgH) 3′α enhancer (3′αE). These sites were defined by oligonucleotide competition in an electrophoretic mobility shift assay (EMSA) and methylation interference footprinting. The 3′αE-binding factor is indistinguishable from NF-HB (B-lineage-specific nuclear factor that binds to the IgH gene) and the B-lineage-specific transcription factor BSAP by several criteria, including similar cell type distribution of binding activity, cross-competition of binding sites in EMSA, similar protein size as demonstrated by UV cross-linking, and sequence identity of one of the 3′αE-binding sites with a BSAP-binding site within the ...
Humans; Animals; Amino Acid Sequence; Molecular Sequence Data; Species Specificity; Protein Structure, Tertiary; Sequence Homology, Amino Acid; Genetic Engineering; Tumor Cells, Cultured; *Genes, Immunoglobulin; Antibodies, Monoclonal/*genetics/immunology; Antibody Affinity; Immunoglobulin Heavy Chains/chemistry/*genetics; Immunoglobulin Variable Region/chemistry/*genetics; Leukemia-Lymphoma, Adult T-Cell; Mice/*genetics/immunology; Recombinant Fusion Proteins/*genetics/immunology. ...
Angiogenesis is a potential prognostic factor in chronic lymphocytic leukemia (CLL). Elevated circulating levels of angiogenic factors in CLL have been repeatedly reported. Nevertheless, the issue of bone marrow neovascularization in CLL remains controversial, partly due to limited number of published studies, different methods of assessing microvessel density (MVD) and small patient cohorts. Moreover, there are very scarce data regarding the relationship of marrow angiogenesis to prognostic markers in CLL. Our objectives were: 1. To assess bone marrow MVD in CLL using two different monoclonal antibodies and a reproducible method of MVD quantification; 2. To examine the possible association of marrow MVD and clinical course, pattern of marrow infiltration, Rai stage, cytogenetic abnormalities detected by fluorescence in situ hybridization (FISH), and mutation status of immunoglobulin heavy chain variable region (IgVH). MVD was higher using CD34 vs vWF antibody ( ...
The intracellular fates of membrane and secretory immunoglobulin heavy chains were examined in a pre-B cell line that has switched to the gamma isotype. The membrane form of the heavy chain (gamma m) was rapidly degraded while the secretory form (gamma s) was retained intracellularly in association with BiP. The degradation of gamma m could not be inhibited by ammonium chloride, chloroquine, or monensin suggesting that it occurred in a nonlysosomal compartment. The inability to detect any Endo H-resistant form of gamma m before its degradation suggested that degradation occurs before entry into the Golgi compartment. Degradation of gamma m could be inhibited by incubation at 24 degrees C. In a derivative of this cell line expressing a transfected kappa gene, gamma s formed disulfide linked tetramers with kappa and was secreted, while gamma m, although associated with kappa, continued to be rapidly degraded. These observations suggest that membrane and secretory heavy chain proteins are retained ...
Nucleotide sequences of immunoglobulin epsilon genes of chimpanzee and orangutan: DNA molecular clock and hominoid evolution.: To determine the phylogenetic rel
Accumulating data have implicated that long noncoding RNA (lncRNA) plays an important role in osteoarthritis (OA), which may function as a competitive endogenous RNA (ceRNA) of microRNAs (miRNAs). lncRNA IGHC|i|γ|/i|1 has been demonstrated to regulate inflammation and autoimmunity. Nonetheless, the altering effect of IGHC|i|γ|/i|1 in OA remains unclear. This study is aimed at investigating the mechanism and function of lncRNA IGHC|i|γ|/i|1 in OA. CCK-8, EdU, and transwell assays were used to estimate macrophage proliferation and migration. Fluorescence in situ hybridization (FISH) was performed to estimate the local expression of lncRNA IGHC|i|γ|/i|1 in macrophages. Luciferase reporter assay was adopted to validate the ceRNA role of IGHC|i|γ|/i|1 as miRNA sponge. lncRNA IGHC|i|γ|/i|1 was primarily localized in macrophage cytoplasm and upregulated in OA. miR-6891-3p inhibited macrophage proliferation, migration, and inflammatory response by targeting TLR4, while
Unique DNA sequences can be determined directly from mouse genomic DNA. A denaturing gel separates by size mixtures of unlabeled DNA fragments from complete restriction and partial chemical cleavages of the entire genome. These lanes of DNA are transferred and UV-crosslinked to nylon membranes. Hybridization with a short 32P-labeled single-stranded probe produces the image of a DNA sequence "ladder" extending from the 3 or 5 end of one restriction site in the genome. Numerous different sequences can be obtained from a single membrane by reprobing. Each band in these sequences represents 3 fg of DNA complementary to the probe. Sequence data from mouse immunoglobulin heavy chain genes from several cell types are presented. The genomic sequencing procedures are applicable to the analysis of genetic polymorphisms, DNA methylation at deoxycytidines, and nucleic acid-protein interactions at single nucleotide resolution.. MeSH Terms ...
The amino acid sequences of the Fd fragments of two human pathological immunoglobulins of the immunoglobulin G1 class are reported. Comparison of the two sequences shows that the heavy-chain variable regions are similar in length to those of the light chains. The existence of heavy chain variable region subgroups is also deduced, from a comparison of these two sequences with those of another γ 1 chain, Eu, a μ chain, Ou, and the partial sequence of a fourth γ 1 chain, Ste. Carbohydrate has been found to be linked to an aspartic acid residue in the variable region of one of the γ 1 chains, Cor.. ...
Immunoglobulins play important roles in antigen recognition during the immune response, and the complementarity-determining region (CDR) 3 of the heavy chain is considered as the critical antigen-binding site. We previously developed a statistical protocol for the extensive analysis of heavy chain variable region repertoires and the dynamics of their immune response using next-generation sequencing (NGS). The properties of important antibody heavy chains predicted in silico by the protocol were examined by gene synthesis and antibody protein expression; however, the corresponding light chain that matches with the heavy chain could not be predicted by our protocol. To understand the dynamics of the heavy chain and the effect of light chain pairing on it, we firstly tried to obtain an artificial light chain that pairs with a broad range of heavy chains and then analyzed its effect on the antigen binding of heavy chains upon pairing. During the pre-B cell stage, the surrogate light chain (SLC) ...
We have developed a novel software algorithm, JOINSOLVER, to analyze the human CDR3H. Within the CDR3H, the definition of the D segment has been particularly problematic because of its short size and extensive terminal processing. Many attempts have been made to define the minimum length needed for D segment assignment (8, 21, 24, 25, 26, 36), yet there is still no consensus definition. Thus, we used novel methods to assign D segments. The first involved the use of a consecutive matching approach rather than the more standard alignment scoring system. The consecutive matching approach permitted the secure assignment of more D segments than the alignment scoring method. The second used methods to limit the search for identity to the VH-JH region only. Finally, a Monte Carlo simulation was used to determine the consecutive match necessary to assign a D segment. We opted to distinguish an actual D segment match from random sequence identity using a 95% probability. This level of confidence seems ...
0110] The following references, to the extent that they provide exemplary procedural or other details supplementary to those set forth herein, are specifically incorporated herein by reference. [0111] U.S. Pat. No. 5,922,591 [0112] U.S. Pat. No. 5,921,396 [0113] U.S. Pat. No. 5,726,012 [0114] U.S. Pat. No. 5,090,420 [0115] Allewell, N. M., Sama, A., "The effect of ammonium sulfate on the activity of ribonuclease A." Biochemica et Biophysica Acta 341:484-488 (1974). [0116] Auffray, C., Rougeon, F., "Purification of mouse immunoglobulin heavy chain messenger RNAs from total myeloma tumor RNA." Eur. J. Biochemistry June;107(2)303-314 (1980). [0117] Boom, W. R., Adriaanse, H., Kievits, T., Lens, P. F., U. S. Pat. No. 5,234,809 entitled: Process for Isolating Nucleic Acid. [0118] Bugos R. C., Chiang, V. L., Zhang, X. H., Campbell, E. R., Podila G. K., Campbell W. H., "RNA isolation from plant tissues recalcitrant to extraction by guanidine." Biotechniques November;19(5)734-7 (1995). [0119] Cairns, M. ...
Toda la información sobre las últimas publicaciones científicas de la Clínica Universidad de Navarra. Acquired potential N-glycosylation sites within the tumor-specific immunoglobulin heavy chains of B-cell malignancies
Thörnqvist L, Ohlin M Data Brief 19 (-) 337-352 [2018-08-00; online 2018-05-04] The highly variable complementary determining region 3 (CDR3) of antibodies is generated through recombination of immunoglobulin heavy chain variable (IGHV), diversity, and joining genes. The codons encoding the first residues of CDR3 may be derived directly from the IGHV germline gene but they may also be generated as part of the rearrangement process. Data of the nucleotide composition of these codons of rearranged genes, an indicator of the degree of contribution of the IGHV gene to CDR3 diversity, are presented in this article. Analyzed data are presented for two unrelated sets of raw sequence data. The raw data sets consisted of sequences of antibody heavy chain-encoding transcripts of six allergic subjects (European Nucleotide Archive accession number PRJEB18926), and paired antibody heavy and light chain variable region-encoding transcripts of memory B cells of three subjects (European Nucleotide Archive ...
Acts as a transcriptional repressor. Binds to E-box sequences in the immunoglobulin heavy chain enhancer as well as in the regulatory regions of many other tissue-specific genes. Represses E-cadherin promoter and induces an epithelial-mesenchymal transition (EMT) by recruiting SMARCA4/BRG1. Represses BCL6 transcription in the presence of the corepressor CTBP1. Positively regulates neuronal differentiation. Represses RCOR1 transcription activation during neurogenesis. Represses transcription by binding to the E box (5-CANNTG-3). Promotes tumorigenicity by repressing stemness-inhibiting microRNAs (By similarity).
Compaction and looping of the ~2.5-Mb Igh locus during V(D)J rearrangement is essential to allow all V(H) genes to be brought in proximity with D(H)-J(H) segments to create a diverse antibody repertoire, but the proteins directly responsible for this are unknown. Because CCCTC-binding factor (CTCF) has been demonstrated to be involved in long-range chromosomal interactions, we hypothesized that CTCF may promote the contraction of the Igh locus. ChIP sequencing was performed on pro-B cells, revealing colocalization of CTCF and Rad21 binding at ~60 sites throughout the V(H) region and 2 other sites within the Igh locus. These numerous CTCF/cohesin sites potentially form the bases of the multiloop rosette structures at the Igh locus that compact during Ig heavy chain rearrangement. To test whether CTCF was involved in locus compaction, we used 3D-FISH to measure compaction in pro-B cells transduced with CTCF shRNA retroviruses. Reduction of CTCF binding resulted in a decrease in Igh locus ...
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V region of the variable domain of immunoglobulin heavy chains that participates in the antigen recognition. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens (PubMed:22158414, PubMed:20176268). The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, ...
Research Interests. Genetic aberrations are important indicators of prognosis in acute lymphoblastic leukaemia (ALL) and are widely used in risk stratification. Translocations involving the immunoglobulin heavy chain locus (IGH) are hallmarks of mature B-cell malignancies, where they drive pathogenesis. IGH translocations have been described in B-cell precursor ALL (BCP-ALL), where they target different genes with the same consequence; the partner gene is overexpressed as a result of its close proximity to the IGH enhancer. We have previously reported recurrent BCP-ALL translocation partner genes including five members of the CCAAT/enhancer binding protein (CEBP) family of transcription factors, showing opposing functions for deregulation in myeloid and lymphoid leukemogenesis; the inhibitory transcription factor, ID4, in the translocation, t(6;14)(p22;q32), defining a subgroup characterized by deletion of CDKN2A/B and PAX; the cytokine receptor for erythropoietin (EPOR) at 19p13; type I ...
Humanized monoclonal antibody (IgG1k) produced by recombinant DNA technology, directed to an epitope in the A antigenic site of the F protein of respiratory syncytial virus (RSV). Synagis is a composite of human (95%) and murine (5%) antibody sequences. The human heavy chain sequence was derived from the constant domains of human IgG1 and the variable framework regions of the VH genes Cor (1) and Cess (2). The human lightchain sequence was derived from the constant domain of Ck and the variable framework regions of the VL gene K104 withJk-4. Palivizumab is expressed from a stable murine (mouse) myeloma cell line (NS0). Palivizumab is composed of to heavy chains (50.6 kDa each) and two light chains (27.6 kDa each), contains 1-2% carbohydrate by weight and has a molecular weight of 147.7 kDa +/- 1 kDa (MALDI-TOF)
SQ P01864 # GCAB_MOUSE Ig gamma-2A chain C region secreted form (B allele) ! SQ P01863 #GCAA_MOUSE Ig gamma-2A chain C region, A allele; 86% sequence identity ! SQ NA # natural chimera; best hits are: SQ P01751 (Ig heavy chain V region B1-8/186-2) and SQ P01864 (Ig gamma-2A chain C region secreted form) ! SQ P01868 # GC1_MOUSE Ig gamma-1 chain C region secreted form ! SQ P01864 # GCAB_MOUSE (P01864) Ig gamma-2A chain C region ! SQ P01837 # KAC_MOUSE (P01837) Ig kappa chain C region ! SQ P01863 # GCAA_MOUSE Ig gamma-2A chain C region, A allele ! SQ NA # part of Fab 28 against HIV-1 RT ! SQ P01868 # ! GC1_MOUSE Ig gamma-1 chain C region secreted form ...
Cells of the monocytic lineage play fundamental roles in the regulation of health, ranging from the initiation and resolution of inflammation to bone homeostasis. In rheumatoid arthritis (RA), the inflamed synovium exhibits characteristic infiltratio
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Tg H chain expression in B cells of heterozygous or homozygous H+L and H3 Tg mice. Cells were isolated from bone marrow (A) and spleen (B) of indicated mice at
Chronic lymphocytic leukemia is a disease with upregulated expression of the transmembrane tyrosine-protein kinase ROR1, a member of the Wnt/planar cell polarity pathway. In this study, we identified COBLL1 as a novel interaction partner of ROR1. COBLL1 shows clear bimodal expression with high levels in chronic lymphocytic leukemia patients with mutated IGHV and approximately 30% of chronic lymphocytic leukemia patients with unmutated IGHV. In the remaining 70% of chronic lymphocytic leukemia patients with unmutated IGHV, COBLL1 expression is low. Importantly, chronic lymphocytic leukemia patients with unmutated IGHV and high COBLL1 have an unfavorable disease course with short overall survival and time to second treatment. COBLL1 serves as an independent molecular marker for overall survival in chronic lymphocytic leukemia patients with unmutated IGHV. In addition, chronic lymphocytic leukemia patients with unmutated IGHV and high COBLL1 show impaired motility and chemotaxis towards CCL19 and ...
Although it has been assumed that the deregulated bcl-2 expression in t(14;18) cells is mediated in part by the immunoglobulin heavy chain gene regulatory region, this has not been demonstrated, nor was it known which elements of that region were responsible for the deregulation. In these studies, we found that the four DNase I-hypersensitive regions within the IgH 3′ enhancer were able to activate the bcl-2 promoter in the t(14;18) cell line DHL-4. Of those four hypersensitive regions, we demonstrated that HS4 had the most influence on bcl-2 promoter activity. This is similar to the situation in pre-B and plasmacytoma cells, where HS4 is the most active enhancer region. We also showed that the HS1,2 region was capable of activating the promoter independently. By itself, HS3 increased bcl-2 promoter activity by only a minor amount. Other studies of HS3 have shown that it is contains elements that act as negative effectors of the IgH 3′ enhancer (37) , and preliminary studies in our ...
Ig epsilon chain C region is a protein that in humans is encoded by the IGHE gene. "Human PubMed Reference:". "Entrez Gene: IGHE immunoglobulin heavy constant epsilon". Venkitaraman AR, Williams GT, Dariavach P, Neuberger MS (Aug 1991). "The B-cell antigen receptor of the five immunoglobulin classes". Nature. 352 (6338): 777-81. doi:10.1038/352777a0. PMID 1881434. Padlan EA, Davies DR (Oct 1986). "A model of the Fc of immunoglobulin E". Molecular Immunology. 23 (10): 1063-75. doi:10.1016/0161-5890(86)90005-2. PMID 3796618. Flanagan JG, Rabbitts TH (1984). "The sequence of a human immunoglobulin epsilon heavy chain constant region gene, and evidence for three non-allelic genes". The EMBO Journal. 1 (5): 655-60. PMC 553102 . PMID 6234164. Max EE, Battey J, Ney R, Kirsch IR, Leder P (Jun 1982). "Duplication and deletion in the human immunoglobulin epsilon genes". Cell. 29 (2): 691-9. doi:10.1016/0092-8674(82)90185-4. PMID 6288268. Ellison J, Buxbaum J, Hood L (1983). "Nucleotide sequence of a human ...
B cell antigen receptor (BCR) or antibody diversity arises from somatic recombination of immunoglobulin (Ig) gene segments and is concentrated within the Ig heavy (H) chain complementarity-determining region 3 (CDR-H3). We performed high-throughput sequencing of the expressed antibody heavy chain repertoire from adult torafugu. We found that torafugu use between 70% and 82% of all possible V (variable) D (diversity) J (joining) gene segment combinations and that they share a similar frequency distribution of these VDJ combinations. The CDR-H3 sequence repertoire observed in individuals is biased with the preferential use of a small number of VDJ, dominated by sequences containing inserted nucleotides. We uncovered the common CDR-H3 amino acid (aa) sequences shared by individuals. Common CDR-H3 sequences feature highly convergent nucleic acid recombination compared with private ones. Finally, we observed differences in repertoires between IgM and IgT, including the unequal usage frequencies of V gene
The invention provides recombinant antibody molecules comprising antigen binding regions derived from the heavy and/or light chain variable regions of a donor anti-CD3 antibody, e.g. OKT3, and which have anti-CD3 binding specificity, preferably of affinity similar to that of OKT3. The recombinant antibody is preferably a humanized antibody and may be a chimeric or CDR-grafted antibody. A method is disclosed for preparing CDR-grafted humanized antibodies in which, in addition to the CDRs, non-human antibody residues are preferably used at positions 23, 24, 49, 71, 73 and 78 of the heavy chain variable region and at positions 46, 48, 58, and 71 of the light chain variable region. The recombinant, especially the humanized, anti-CD3 antibodies may used for in vivo therapy or diagnosis.
ZytuxTM is a biosimilar product with the generic name of Rituximab. It is a genetically engineered human-mouse chimeric monoclonal antibody produced by Chinese Hamster Ovary (CHO) cells in suspension. It is a fusion of the light and heavy chain variable domains of a murine monoclonal anti-CD20 antibody and human kappa light-chain and gamma 1 heavy-chain constant regions. Rituximab is a chimeric monoclonal antibody against CD20 antigens presents on surface of lymphocyte cells. Rituximab binds to the target CD20 antigen via the variable murine regions, while the remainder of the antibody interacts with human immune-effector mechanisms to kill the target cells. Zytux™ is used for treatment of ...
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To promote cancer research and to develop innovative therapies, refined pre-clinical mouse tumor models that mimic the actual disease in humans are of dire need. A number of neoplasms along the B cell lineage are commonly initiated by a translocation recombining c-myc with the immunoglobulin heavy-chain gene locus. The translocation is modeled in the C.129S1-Ighatm1(Myc)Janz/J mouse which has been previously engineered to express c-myc under the control of the endogenous IgH promoter. This transgenic mouse exhibits B cell hyperplasia and develops diverse B cell tumors. We have isolated tumor cells from the spleen of a C.129S1-Ighatm1(Myc)Janz/J mouse that spontaneously developed a plasmablastic lymphoma-like disease. These cells were cultured, transduced to express eGFP and firefly luciferase, and gave rise to a highly aggressive, transplantable B cell lymphoma cell line, termed IM380. This model bears several advantages over other models as it is genetically induced and mimics the translocation ...
Rat IgG2b Kappa Light Chain Isotype Control (149/10H5) [PE/Cy7]. Available in 26 dyes & fluorophores. Backed by our 100% Guarantee.
Hi all, Im a beginner to bioinformatics, and Im having trouble finding specific tools that could help me analyze and visualize data of immunoglobulin variable heavy chains. The data I have is from a MiSeq readout. So far Ive only been able to use PEAR to sticth my reads together, FASTAptamer to count, compare, and cluster my sequences, and thats about it. Ive been trying to use different visualization tools on Galaxy but it seems like the ones that do allow FASTA files as input are spitting out errors because of varying length of sequences in the file and other errors (one that keeps popping up is "Picked Up JAVA_OPTIONS"?). Ive tried IgGalaxy but Im having trouble setting it up because the VMWare isnt compatible with my computer. Am I missing something with Galaxy that Im not aware of? Thanks in advance!. ...
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The genesis of human follicular lymphoma (FL) is a multistep process. The initial event is thought to be the chromosomal translocation t(14;18)(q32;q21) juxtaposing the bcl-2 proto-oncogene with the immunoglobulin (Ig) H chain locus joining segment (JH) as an error of D-J or V-D joining in the pre-B cell. However, FL is recognized clinically as a tumor of surface Ig (sIg)-positive B cells with morphologic and phenotypic similarities to the centrocyte of the secondary immune response. Thus, additional steps must be involved in the clonal expansion of the FL tumor cell beyond the activation of bcl-2 as a consequence of the t(14;18) translocation. Like the normal centrocyte, somatic mutations accumulate in the variable (V) genes of FL tumor B cells. To determine if clonal expansion of FL occurs before or after the development of the malignant follicle, we sought to examine the evolution of the FL V gene from its unmutated germline (GL) counterpart. To obtain the GL gene we first cloned the ...
results of an open-label, multicenter, phase I/II trial aiming to determine the Maximal Tolerable Dose (MTD) of alemtuzumab consolidation and to evaluate safety and efficacy in CLL patients who responded to second-line fludarabine-based treatment.
This trial was investigating the efficacy, pharmacokinetics, tolerability and quality-of-life effects of rituximab in patients with chronic lymphocytic
A role of B cells in multiple sclerosis (MS) is well established, but there is limited understanding of their involvement during active disease. Here, we examined cerebrospinal fluid (CSF) and peripheral blood (PB) B cells in treatment-naive patients with MS or high-risk clinically isolated syndrome. Using flow cytometry, we found increased CSF lymphocytes with a disproportionate increase of B cells compared with T cells in patients with gadolinium-enhancing (Gd+) lesions on brain MRI. Ig gene heavy chain variable region (Ig-VH) repertoire sequencing of CSF and PB B cells revealed clonal relationships between intrathecal and peripheral B cell populations, which could be consistent with migration of B cells to and activation in the CNS in active MS ...
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