TY - JOUR. T1 - Assembly and retention of CD1b heavy chains in the endoplasmic reticulum. AU - Sugita, Masahiko. AU - Porcelli, Steven A.. AU - Brenner, Michael B.. PY - 1997/12/1. Y1 - 1997/12/1. N2 - The endoplasmic reticulum (ER) is the site for assembly of MHC class I molecules. Newly synthesized class I heavy chains bind calnexin, an ER-resident molecular chaperone, and dissociate from calnexin following association with β2-microglobulin (β2m). The class I heavy chain:β2m complex then is stabilized by binding endogenous peptides transported to the ER through the TAP molecules. Thus, both β2m and TAP are required for class I Ag presentation. Human CD1b is a β2m-associated, non-MHC-encoded glycoprotein that functions in presentation of lipid Ags to T cells. Despite its structural similarities with class I, CD1b-mediated Ag presentation is TAP independent, and CD1b traffics to endocytic compartments for sampling exogenous Ags. Given these distinctive features of CD1b, we set out to ...

Anti-Mouse IgG2b heavy chain Unconjugated preadsorbed (cross-adsorbed) validated for WB, ELISA, IHC-P, ICC. Other Unconjugated secondaries available.

The heavy chain diseases (HCDs) are B-cell proliferative disorders characterized by production of abnormal, structurally incomplete, immunoglobulin heavy chains without the corresponding light chains. The abnormal HCD proteins are the result of gene mutations, deletions, or insertions.

heavy chain disease; Franklin Disease; gamma-Chain Disease; mu-Chain Disease. On-line free medical diagnosis assistant. Ranked list of possible diseases from either several symptoms or a full patient history. A similarity measure between symptoms and diseases is provided.

Published on 4/25/2012. Tan JN, Kroll MH, OHara CJ, Everett PC, Erdogan E. Gamma heavy chain disease in a patient with underlying lymphoplasmacytic lymphoma of the thyroid. Report of a case and comparison with other reported cases with thyroid involvement. Clin Chim Acta. 2012 Oct 9; 413(19-20):1696-9. PMID: 22561184.. Read at: PubMed ...

A disorder of immunoglobulin synthesis in which large quantities of abnormal heavy chains are excreted in the urine. The amino acid sequences of the N-(amino-) terminal regions of these chains are normal, but they have a deletion extending from part of the variable domain through the first domain of the constant region, so that they cannot form cross-links to the light chains. The defect arises through faulty coupling of the variable (V) and constant (C) region genes ...

The amino acid sequence of fragments obtained by cyanogen bromide cleavage of the mu-chain of a human γM-globulin is homologous to the NH2-terminal sequences of the gamma-chain of human and rabbit γG-globulins and is related to that of human light chains. This supports the hypothesis that light and heavy chains evolved from a common ancestral gene. ...

Rabbit anti-mouse IgG recognizes mouse IgG gamma heavy chain. This secondary antibody was purified using antigen affinity chromatography. Mouse IgG gamma heavy chain (PAB9361) - Products - Abnova

Rhesus macaques represent a valuable model in biomedical research and in development of vaccines and therapeutics. Due to the lack of reagents, the general properties of IgG and corresponding cellular receptors (FcγR) in this species are poorly characterized. We engineered recombinant IgGs containing each of the four rhesus macaque heavy constant region (CH) subclasses. To define FcγRs that mediate IgGs, we identified and characterized three FcγR classes, and generated recombinant cDNA constructs. cDNA IgH constructs were created by fusing - by sequence overlap extension PCRs - a gene segment encoding the murine variable heavy domain specific for the hapten NIP, an established specificity system for assessing antibody effector functions, with rhesus macaque CH fragments. The complete IgH constructs were transfected into J558L cells, a murine IgH-lost myeloma cell line expressing anti-NIP light chain. Secretion of engineered IgGs was determined by ELISAs using NIP-BSA and anti-monkey IgG-specific

The contribution to the decay $b\to s \gamma$ in the Minimal Supersymmetric Standard Model with radiative electroweak breaking is presented. The impact of a detection of this inclusive decay at the SM level is discussed. Results for other inclusive $b-s$ transitions are also mentioned. |P /|

Recombinant Human CPE (Accession # NP_001864) Arg42-Ser453, fused with a C-terminal 6-His tag, was produced in Mouse myeloma cell line, NS0-derived.

Using cosmids covering about 117 Kb upstream of the human immunoglobulin chain C mu gene, we have identified a potentially functional VH gene, belonging to the VHVI subgroup. This VHVI gene is only about 95 Kb from the C mu gene and is probably the first functional VH segment of the Igh locus. These results illustrate the proximity of the human VH, DH and JH segments involved in creation of the complete heavy chain genes.

Free, official coding info for 2021 ICD-10-CM C88.2 - includes detailed rules, notes, synonyms, ICD-9-CM conversion, index and annotation crosswalks, DRG grouping and more.

Depending on whether the nature of the antigen is protein or polysaccharide and the frequency and duration of stimulation by the antigen, an antibody response may exhibit changes in the distribution of IgG subclasses in plasma, and cause increased or diminished levels of one or more IgG subclasses (Meulenbroek et al., 2000). When the serum level of a subclass is below detection levels of the most sensitive techniques (ELISA/RIA), it is considered as a complete deficiency /absence or a total lack (Meulenbroek et al., 2000). Such complete deficiency is rare and is usually due to deletions in chromosome 14 loci. Such a total lack of one or more IgG subclasses due to deletions of the immunoglobulin heavy chain constant region genes is occasionally found in healthy individuals. The fact that these individuals still produce protective antibody titers in the residual immunoglobulin classes or subclasses suggests that the deletion of the isotype(s) occurs by chance and can be compensated adequately ...

A macrophage activator, IL-4 stimulates phagocytic activity and MHC class II gene expression. IL-4 stimulates isotype switching by activating the promoters for Iε, and Iγ1 (the I regions for ε and γ1 heavy chain constant region genes). IL-4 is pivotal in regulating the IgE response: IgG1 and IgE account ∼2% of all antibodies secreted by splenic B cells incubated with LPS; IgG1 accounts for ∼50% and IgE accounts for ∼20% of all antibodies secreted by B cells incubated with LPS and IL-4. IL-4 knockout mice cannot mount an IgE response to parasites. Also, CD4 T cells activated in presence of IL-4 develop into TH2 cells (especially if IL-6 is also present); IL-4 and IL-10 both inhibit T cell differentiation into TH1 cells ...

Booth Profile ArkAb develops, manufactures and markets raw materials for in vitro diagnostics assays. Our products are human/mouse chimeric monoclonal antibodies (human heavy chain constant region). They are used as an alternative to disease state plasma for immunoassay quality controls (positive controls, quality controls and calibrators). ArkAb catalog includes products for infectious diseases, auto-immunity, allergies and food intolerances. The company also offers custom made development services.. ...

Standard laboratory tests to evaluate organ function will be used to assess adverse effects on organ systems and function. Electrocardiograms will be recorded to assess any effect on cardiac conduction. Subjects will be monitored for any clinical adverse signs at least monthly, and more frequently if patient condition warrants. In each case of reported adverse events, an assessment will be made if the event is due to EPI-743 administration or to underlying/intercurrent disease ...

Looking for online definition of alpha heavy chain disease in the Medical Dictionary? alpha heavy chain disease explanation free. What is alpha heavy chain disease? Meaning of alpha heavy chain disease medical term. What does alpha heavy chain disease mean?

Buy our Recombinant Human heavy chain Myosin protein. Ab114308 is a protein fragment produced in Wheat germ and has been validated in WB, ELISA, SDS-PAGE…

Functional isotypes are not encoded by the constant region genes of the beta subunit of the T cell receptor for antigen/major histocompatibility complex ...

[ChEMBL Target Description] ID:CHEMBL5842, Name:Low affinity immunoglobulin gamma Fc region receptor III-B, Description:, Synonyms:

data a; run; ods graphics on; ods select DensityPanel; proc mcmc data=a stats=none diag=none nmc=10000 outpost=gout plots=density seed=1; parms gamma_3_is2 gamma_001_sc4 igamma_12_sc001 igamma_2_is01; prior gamma_3_is2 ~ gamma(shape=3, iscale=2); prior gamma_001_sc4 ~ gamma(shape=0.01, scale=4); prior igamma_12_sc001 ~ igamma(shape=12, scale=0.01); prior igamma_2_is01 ~ igamma(shape=2, iscale=0.1); model general(0); run; ods graphics off ...

India :: occurs only in Est 1:1 and Est 8:9, where the extent of the dominion of the Persian king is described. The country so designated here is not the

Ig epsilon chain C region is a protein that in humans is encoded by the IGHE gene. Human PubMed Reference:. Entrez Gene: IGHE immunoglobulin heavy constant epsilon. Venkitaraman AR, Williams GT, Dariavach P, Neuberger MS (Aug 1991). The B-cell antigen receptor of the five immunoglobulin classes. Nature. 352 (6338): 777-81. doi:10.1038/352777a0. PMID 1881434. Padlan EA, Davies DR (Oct 1986). A model of the Fc of immunoglobulin E. Molecular Immunology. 23 (10): 1063-75. doi:10.1016/0161-5890(86)90005-2. PMID 3796618. Flanagan JG, Rabbitts TH (1984). The sequence of a human immunoglobulin epsilon heavy chain constant region gene, and evidence for three non-allelic genes. The EMBO Journal. 1 (5): 655-60. PMC 553102 . PMID 6234164. Max EE, Battey J, Ney R, Kirsch IR, Leder P (Jun 1982). Duplication and deletion in the human immunoglobulin epsilon genes. Cell. 29 (2): 691-9. doi:10.1016/0092-8674(82)90185-4. PMID 6288268. Ellison J, Buxbaum J, Hood L (1983). Nucleotide sequence of a human ...

HEADER HYDROLASE/HYDROLASE INHIBITOR 11-MAY-10 3AI8 TITLE CATHEPSIN B IN COMPLEX WITH THE NITROXOLINE COMPND MOL_ID: 1; COMPND 2 MOLECULE: CATHEPSIN B; COMPND 3 CHAIN: B, A; COMPND 4 SYNONYM: CATHEPSIN B1, APP SECRETASE, APPS, CATHEPSIN B LIGHT CHAIN, COMPND 5 CATHEPSIN B HEAVY CHAIN; COMPND 6 EC: 3.4.22.1; COMPND 7 ENGINEERED: YES SOURCE MOL_ID: 1; SOURCE 2 ORGANISM_SCIENTIFIC: HOMO SAPIENS; SOURCE 3 ORGANISM_COMMON: HUMAN; SOURCE 4 ORGANISM_TAXID: 9606; SOURCE 5 GENE: CTSB; SOURCE 6 EXPRESSION_SYSTEM: ESCHERICHIA COLI; SOURCE 7 EXPRESSION_SYSTEM_TAXID: 562; SOURCE 8 EXPRESSION_SYSTEM_STRAIN: BL21DE3; SOURCE 9 EXPRESSION_SYSTEM_VECTOR_TYPE: PLASMID; SOURCE 10 EXPRESSION_SYSTEM_PLASMID: PET3A KEYWDS CATHEPSIN B, REVERSIBLE INHIBITOR, NITROXOLINE, 8-HYDROXY-5- KEYWDS 2 NITROQUINOLINE, HYDROLASE-HYDROLASE INHIBITOR COMPLEX EXPDTA X-RAY DIFFRACTION AUTHOR M.RENKO,B.MIRKOVIC,S.GOBEC,J.KOS,D.TURK REVDAT 3 29-JAN-14 3AI8 1 JRNL VERSN REVDAT 2 08-JUN-11 3AI8 1 JRNL REVDAT 1 18-MAY-11 3AI8 0 JRNL AUTH ...

The invention discloses a multi-well plate guide protector for single- and multi-well dispensing in a multi-well plate. The guide protector comprises a protecting cover having a height and dimension a

In spite of the strong expression of Wnt-10b in melanomas, its role in melanoma cells has not been elucidated. In the present study, the biological effects of Wnt-10b on murine B16F10 (B16) melanoma cells were investigated using conditioned medium from Wnt-10b-producing COS cells (Wnt-CM). After 2 days of culture in the presence of Wnt-CM, proliferation of B16 melanoma cells was inhibited, whereas ...

PP1C gamma antibody [2F2-A3-H10] (protein phosphatase 1, catalytic subunit, gamma isozyme) for WB. Anti-PP1C gamma mAb (GTX49165) is tested in Human samples. 100% Ab-Assurance.

Bacillus anthracis, the causative agent of anthrax, is surrounded by an antiphagocytic capsule composed of poly-£^-D-glutamic acid (£^DPGA). Antibodies directed against the £^DPGA capsule are opsonic and protective, which makes the capsule an attractive target for vaccine development. However, antibodies of different IgG subclasses differ in their ability to activate effector functions, including activation of complement and phagocytosis. In addition, recent studies found differences in affinity and protective activity between variable region identical IgG subclass switch monoclonal antibodies (mAbs) directed against microbial capsules or outer membrane proteins. We recently generated six mAbs against the £^DPGA capsule and found that only mAbs of IgG3 subclass were protective in a murine model of inhalational anthrax. In order to investigate the role of IgG subclass to protective activity against anthrax, the goal of the study was to generate a full IgG subclass switch family (IgG3 ,,³ ...

Required for TCR (T-cell antigen receptor)- and pre-TCR-mediated signaling, both in mature T-cells and during their development. Involved in FCGR3 (low affinity immunoglobulin gamma Fc region receptor III)-mediated signaling in natural killer cells and FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. Couples activation of these receptors and their associated kinases with distal intracellular events such as mobilization of intracellular calcium stores, PKC activation, MAPK activation or cytoskeletal reorganization through the recruitment of PLCG1, GRB2, GRAP2, and other signaling molecules.

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Mu heavy chain disease (HCD) is the most rare variant of HCD, a family of syndromes associated with or representing a B cell malignancy variant. The hallmark characteristic and the pathogenic mechanism of HCD is the synthesis of a mutant, misfolded immunoglobulin heavy chain (IgH) which cannot form a quaternary conformation with the immunoglobulin light chain (IgL) and/or be degraded by the proteasome. The isotype of mutated IgH (α,γ or μ) determines the nomenclature of HCD subtypes. Less than 50 cases of mu HCD have been reported. The first two cases of mu HCD were described in the 1970s. The disease was diagnosed in men in their late fifties complaining of unremitting joint pain/stiffness. Mu HCD affects predominantly Caucasian men in their 5th-6th decades. Similar to the other HCD, the etiopathogenesis of mu HCD is unknown, but most patients have a concurrent lymphoproliferative disorder resembling chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL). There are single case ...

Mu heavy chain disease (HCD) is the most rare variant of HCD, a family of syndromes associated with or representing a B cell malignancy variant. The hallmark characteristic and the pathogenic mechanism of HCD is the synthesis of a mutant, misfolded immunoglobulin heavy chain (IgH) which cannot form a quaternary conformation with the immunoglobulin light chain (IgL) and/or be degraded by the proteasome. The isotype of mutated IgH (α,γ or μ) determines the nomenclature of HCD subtypes. Less than 50 cases of mu HCD have been reported. The first two cases of mu HCD were described in the 1970s. The disease was diagnosed in men in their late fifties complaining of unremitting joint pain/stiffness. Mu HCD affects predominantly Caucasian men in their 5th-6th decades. Similar to the other HCD, the etiopathogenesis of mu HCD is unknown, but most patients have a concurrent lymphoproliferative disorder resembling chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL). There are single case ...

Nocardia jinanensis sp. nov., an amicoumacin B-producing actinomycete.: A novel actinomycete, strain 04-5195(T), that produces amicoumacin B, which targets bone

A recombinant chimeric (murine/human) monoclonal antibody (IgG1k) that functions as an immunosuppressive agent, specifically binding to and blocking the interleukin-2 receptor a-chain (IL-2R alpha, also known as CD25 antigen) on the surface of activated T-lymphocytes. It is a 144 kDa glycoprotein obtained from fermentation of an established mouse myeloma cell line genetically engineered to express plasmids containing the human heavy and light chain constant region genes and mouse heavy and light chain variable region genes encoding the RFT5 antibody that binds selectively to the IL-2R alpha.

isotype switching will change the isotype of the Ab. The constant region of the Ab is coded by the C region with the greek letter that matches its name. Ie, IgG has a C(gamma) constant region ...

Immunoglobulins produced by B cells are composed of two heavy and two light chains, connected by disulfide bonds. Differences in the composition of the heavy chain constant regions determine the isotype of the antibody. The light chains of all isotypes are either of κ or λ subtype. Clone IS7-24C7 detects human Ig light chains of the λ subtype. Heavy chains and light chains of the κ subtype are not detected. - France

This protein functions as an embryonic globin, but the gene structure and chromosomal location resemble more closely the human gamma chain gene, which codes for a fetal globin.

Clone REA589 recognizes the human CD16b antigen, a glycosylphosphatidylinositol-anchored protein known as low affinity immunoglobulin gamma Fc region receptor III-B. CD16 is expressed in two different isoforms, FcγRIIIa (CD16a), which is found on human NK cells, macrophages, and mast cells, and FcγRIIIb (CD16b) Fc receptors, that bind to the Fc portion of IgG antibodies which mediates NK cell activating. CD16b is polymorphic and has two codominant alleles that are related to Neutrophil Antigen 1 (NA1) and Neutrophil Antigen 2 (NA2). Clone REA589 is present on polymorphonuclear leukocytes (neutrophils) expressing the NA1 molecule.Additional information: Clone REA589 displays negligible binding to Fc receptors. - Nederland

MPC-3100 is an orally bioavailable, synthetic, second-generation small-molecule inhibitor of heat shock protein 90 (Hsp90) with potential antineoplastic activity.

DNA encoding the rat diversity segment (D), joining segment (JH), and constant (C) region mu, gamma 2a, gamma 1, gamma 2b, epsilon and alpha of the Ig heavy chain has been isolated from a cosmid library. Restriction mapping allowed us to identify two gene clusters: D-JH-C mu and C gamma 1-C gamma 2b-C epsilon-C alpha in addition to a single C gamma 2a gene. Analysis of genomic DNA by Southern blotting permitted identification of the C gamma 2c gene and led to the proposal of the following gene order for the rat Ig heavy chain locus: D-JH-C mu-C delta-(C gamma 2c, C gamma 2a)-C gamma 1-C gamma 2b-C epsilon-C alpha. There is striking homology between the rat and mouse Ig heavy chain loci as regards gene order and distance between CH genes. Partial DNA sequencing confirms this homology and shows that exon sequences are more conserved than are intron sequences. One of the most conserved intron regions between rat and mouse is that spanning the Ig heavy chain enhancer (91% homology). However, the

Looking for online definition of Bence-Jones proteinuria in the Medical Dictionary? Bence-Jones proteinuria explanation free. What is Bence-Jones proteinuria? Meaning of Bence-Jones proteinuria medical term. What does Bence-Jones proteinuria mean?

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Humanized monoclonal antibody (IgG1k) produced by recombinant DNA technology, directed to an epitope in the A antigenic site of the F protein of respiratory syncytial virus (RSV). Synagis is a composite of human (95%) and murine (5%) antibody sequences. The human heavy chain sequence was derived from the constant domains of human IgG1 and the variable framework regions of the VH genes Cor (1) and Cess (2). The human lightchain sequence was derived from the constant domain of Ck and the variable framework regions of the VL gene K104 withJk-4. Palivizumab is expressed from a stable murine (mouse) myeloma cell line (NS0). Palivizumab is composed of to heavy chains (50.6 kDa each) and two light chains (27.6 kDa each), contains 1-2% carbohydrate by weight and has a molecular weight of 147.7 kDa +/- 1 kDa (MALDI-TOF)

Human gamma globulin when administered intraperitoneally to mice challenged by a lethal intracerebral staphylococcal infection, enhanced the protective action of penicillin and oxytetracycline. This fact, added to the previously described antistaphylococcal action of human gamma globulin used in experimental and human infections suggest that human gamma globulin could be tried with some success as a preventive agent in human groups particularly exposed to staphylococcal infection, such as the prematurely born babies.. ...