Using cosmids covering about 117 Kb upstream of the human immunoglobulin chain C mu gene, we have identified a potentially functional VH gene, belonging to the VHVI subgroup. This VHVI gene is only about 95 Kb from the C mu gene and is probably the first functional VH segment of the Igh locus. These results illustrate the proximity of the human VH, DH and JH segments involved in creation of the complete heavy chain genes.
Bacillus anthracis, the causative agent of anthrax, is surrounded by an antiphagocytic capsule composed of poly-£^-D-glutamic acid (£^DPGA). Antibodies directed against the £^DPGA capsule are opsonic and protective, which makes the capsule an attractive target for vaccine development. However, antibodies of different IgG subclasses differ in their ability to activate effector functions, including activation of complement and phagocytosis. In addition, recent studies found differences in affinity and protective activity between variable region identical IgG subclass switch monoclonal antibodies (mAbs) directed against microbial capsules or outer membrane proteins. We recently generated six mAbs against the £^DPGA capsule and found that only mAbs of IgG3 subclass were protective in a murine model of inhalational anthrax. In order to investigate the role of IgG subclass to protective activity against anthrax, the goal of the study was to generate a full IgG subclass switch family (IgG3 ,,³ ...
Depending on whether the nature of the antigen is protein or polysaccharide and the frequency and duration of stimulation by the antigen, an antibody response may exhibit changes in the distribution of IgG subclasses in plasma, and cause increased or diminished levels of one or more IgG subclasses (Meulenbroek et al., 2000). When the serum level of a subclass is below detection levels of the most sensitive techniques (ELISA/RIA), it is considered as a complete deficiency /absence or a total lack (Meulenbroek et al., 2000). Such complete deficiency is rare and is usually due to deletions in chromosome 14 loci. Such a total lack of one or more IgG subclasses due to deletions of the immunoglobulin heavy chain constant region genes is occasionally found in healthy individuals. The fact that these individuals still produce protective antibody titers in the residual immunoglobulin classes or subclasses suggests that the deletion of the isotype(s) occurs by chance and can be compensated adequately ...
Immune systems of vertebrates function via two types of effector cells, B and T cells, which are capable of antigen-specific recognition. The immunoglobulins, which serve as antigen receptors on B cells, have been well characterized with respect to gene structure, unlike the T-cell receptors. Recently, cDNA clones thought to correspond to the beta-chain locus of the human and mouse T-cell receptor have been described. The presumptive beta-chain clones detect gene rearrangement specifically in T-cell DNA and show homology with immunoglobulin light chains. The similarity of the T-cell beta-chain gene system to the immunoglobulin genes has been further demonstrated by the recent observation of variable- and constant-region gene segments as well as joining segments and putative diversity segments. We report here the characterization of cDNA and genomic clones encoding human T-cell receptor beta-chain genes. There are two constant-region genes (C beta 1 and C beta 2), each capable of rearrangement and
A macrophage activator, IL-4 stimulates phagocytic activity and MHC class II gene expression. IL-4 stimulates isotype switching by activating the promoters for Iε, and Iγ1 (the I regions for ε and γ1 heavy chain constant region genes). IL-4 is pivotal in regulating the IgE response: IgG1 and IgE account ∼2% of all antibodies secreted by splenic B cells incubated with LPS; IgG1 accounts for ∼50% and IgE accounts for ∼20% of all antibodies secreted by B cells incubated with LPS and IL-4. IL-4 knockout mice cannot mount an IgE response to parasites. Also, CD4 T cells activated in presence of IL-4 develop into TH2 cells (especially if IL-6 is also present); IL-4 and IL-10 both inhibit T cell differentiation into TH1 cells ...
A recombinant chimeric (murine/human) monoclonal antibody (IgG1k) that functions as an immunosuppressive agent, specifically binding to and blocking the interleukin-2 receptor a-chain (IL-2R alpha, also known as CD25 antigen) on the surface of activated T-lymphocytes. It is a 144 kDa glycoprotein obtained from fermentation of an established mouse myeloma cell line genetically engineered to express plasmids containing the human heavy and light chain constant region genes and mouse heavy and light chain variable region genes encoding the RFT5 antibody that binds selectively to the IL-2R alpha.
Immunoglobulin constant region FC receptor binding agents | Anti-NPC2 monoclonal antibodies and a method of detecting fatty liver tissue, cancer cells or cancer tissue by... | Anti-lipoarabinomannan antibody and immunoassay for acid-fast bacillary infection using the antibody | Monoclonal antibodies to anthrax protective antigen | Humanized pertussis antibodies and uses thereof |
In recent years, the extent of our vulnerability to misinterpretation due to poorly characterized reagents has become an issue of great concern. Antibody reagents have been identified as a major source of error, contributing to the reproducibility crisis. In the current report, we define an additional dimension of the crisis; in particular, we define variation of the targets being analyzed. We report that natural variation in the immunoglobulin constant region alters the reactivity with commonly used subtype-specific anti-IgG reagents, resulting in cross-reactivity of polyclonal regents with inappropriate targets and blind spots of monoclonal reagents for desired targets. This raises the practical concern that numerous studies characterizing IgG subtypes in human disease may contain errors due to such previously unappreciated defects. These studies also focus attention on the broader concern that genetic variation may affect the performance of any laboratory or research test that uses ...
Functional isotypes are not encoded by the constant region genes of the beta subunit of the T cell receptor for antigen/major histocompatibility complex ...
human 11-1f4 monoclonal antibody: amyloid-reactive antibody; was produced in CHOdhfr-stable mammalian cell lines that had been transfected with a supervector DNA encoding the mouse 11-1F4 heavy and light chain variable regions (V(H), V(L)) and human heavy and light chain constant regions (C(H), C(L))
Booth Profile ArkAb develops, manufactures and markets raw materials for in vitro diagnostics assays. Our products are human/mouse chimeric monoclonal antibodies (human heavy chain constant region). They are used as an alternative to disease state plasma for immunoassay quality controls (positive controls, quality controls and calibrators). ArkAb catalog includes products for infectious diseases, auto-immunity, allergies and food intolerances. The company also offers custom made development services.. ...
isotype switching will change the isotype of the Ab. The constant region of the Ab is coded by the C region with the greek letter that matches its name. Ie, IgG has a C(gamma) constant region ...
New sequencing data show that the Mcg-Ke+Oz- isotype is encoded by a polymorphic C lambda 2 gene segment that differs from the normal J-C lambda 2 region at a single nucleotide position ...
Ig epsilon chain C region is a protein that in humans is encoded by the IGHE gene. Human PubMed Reference:. Entrez Gene: IGHE immunoglobulin heavy constant epsilon. Venkitaraman AR, Williams GT, Dariavach P, Neuberger MS (Aug 1991). The B-cell antigen receptor of the five immunoglobulin classes. Nature. 352 (6338): 777-81. doi:10.1038/352777a0. PMID 1881434. Padlan EA, Davies DR (Oct 1986). A model of the Fc of immunoglobulin E. Molecular Immunology. 23 (10): 1063-75. doi:10.1016/0161-5890(86)90005-2. PMID 3796618. Flanagan JG, Rabbitts TH (1984). The sequence of a human immunoglobulin epsilon heavy chain constant region gene, and evidence for three non-allelic genes. The EMBO Journal. 1 (5): 655-60. PMC 553102 . PMID 6234164. Max EE, Battey J, Ney R, Kirsch IR, Leder P (Jun 1982). Duplication and deletion in the human immunoglobulin epsilon genes. Cell. 29 (2): 691-9. doi:10.1016/0092-8674(82)90185-4. PMID 6288268. Ellison J, Buxbaum J, Hood L (1983). Nucleotide sequence of a human ...
ZytuxTM is a biosimilar product with the generic name of Rituximab. It is a genetically engineered human-mouse chimeric monoclonal antibody produced by Chinese Hamster Ovary (CHO) cells in suspension. It is a fusion of the light and heavy chain variable domains of a murine monoclonal anti-CD20 antibody and human kappa light-chain and gamma 1 heavy-chain constant regions. Rituximab is a chimeric monoclonal antibody against CD20 antigens presents on surface of lymphocyte cells. Rituximab binds to the target CD20 antigen via the variable murine regions, while the remainder of the antibody interacts with human immune-effector mechanisms to kill the target cells. Zytux™ is used for treatment of ...
Immunoglobulins produced by B cells are composed of two heavy and two light chains, connected by disulfide bonds. Differences in the composition of the heavy chain constant regions determine the isotype of the antibody. The light chains of all isotypes are either of κ or λ subtype. Clone IS7-24C7 detects human Ig light chains of the λ subtype. Heavy chains and light chains of the κ subtype are not detected. - France
Shop Ig gamma-2 chain C region ELISA Kit, Recombinant Protein and Ig gamma-2 chain C region Antibody at MyBioSource. Custom ELISA Kit, Recombinant Protein and Antibody are available.
Shop Ig lambda-3 chain C region ELISA Kit, Recombinant Protein and Ig lambda-3 chain C region Antibody at MyBioSource. Custom ELISA Kit, Recombinant Protein and Antibody are available.
Lenti ORF particles, Igk (Myc-DDK-tagged) - Mouse immunoglobulin kappa chain, constant region (cDNA clone MGC:102611 IMAGE:4219669), 200 uL, |10^7 TU/mL, 200 µl.
Infliximab is a purified, recombinant DNA-derived chimeric human-mouse IgG monoclonal antibody that consists of mouse heavy and light chain variable regions combined with human heavy and light chain constant regions.[25] It has a serum half-life of 9.5 days and can be detected in serum 8 weeks after infusion treatment.[25] Infliximab neutralizes the biological activity of TNF-α by binding with high affinity to the soluble (free floating in the blood) and transmembrane (located on the outer membranes of T cells and similar immune cells) forms of TNF-α, and inhibits or prevents the effective binding of TNF-α with its receptors. Infliximab and adalimumab (another TNF antagonist) are in the subclass of anti-TNF antibodies (they are in the form of naturally occurring antibodies), and are capable of neutralizing all forms (extracellular-, transmembrane-, and receptor-bound) TNF-α.[26] Etanercept, a third TNF antagonist, is in a different subclass (receptor-construct fusion protein), and, because ...
Infliximab is a purified, recombinant DNA-derived chimeric human-mouse IgG monoclonal antibody that consists of mouse heavy and light chain variable regions combined with human heavy and light chain constant regions.[25] It has a serum half-life of 9.5 days and can be detected in serum 8 weeks after infusion treatment.[25]. Infliximab neutralizes the biological activity of TNF-α by binding with high affinity to the soluble (free floating in the blood) and transmembrane (located on the outer membranes of T cells and similar immune cells) forms of TNF-α, and inhibits or prevents the effective binding of TNF-α with its receptors. Infliximab and adalimumab (another TNF antagonist) are in the subclass of anti-TNF antibodies (they are in the form of naturally occurring antibodies), and are capable of neutralizing all forms (extracellular-, transmembrane-, and receptor-bound) TNF-α.[26] Etanercept, a third TNF antagonist, is in a different subclass (receptor-construct fusion protein), and, ...
The immunoglobulin kappa light-chain constant region gene (C,) has been cloned and sequenced from five wild mouse species. Analysis of these data has permitted an assessment of single-copy gene evolution during a limited time period as defined by the genus MUX Sequence conservation was found to be as high (or higher) in the 5 and enhancer regions as in the coding region. The pattern of substitutions throughout these genes suggests that parallel evolution has occurred frequently and that substitutions at replacement sites have not decreased significantly, owing to saturation during this period of 10 Myr. Phylogenetic relationships have been determined among these wild species as well as among members of the genus Rat&s.
SQ P01864 # GCAB_MOUSE Ig gamma-2A chain C region secreted form (B allele) ! SQ P01863 #GCAA_MOUSE Ig gamma-2A chain C region, A allele; 86% sequence identity ! SQ NA # natural chimera; best hits are: SQ P01751 (Ig heavy chain V region B1-8/186-2) and SQ P01864 (Ig gamma-2A chain C region secreted form) ! SQ P01868 # GC1_MOUSE Ig gamma-1 chain C region secreted form ! SQ P01864 # GCAB_MOUSE (P01864) Ig gamma-2A chain C region ! SQ P01837 # KAC_MOUSE (P01837) Ig kappa chain C region ! SQ P01863 # GCAA_MOUSE Ig gamma-2A chain C region, A allele ! SQ NA # part of Fab 28 against HIV-1 RT ! SQ P01868 # ! GC1_MOUSE Ig gamma-1 chain C region secreted form ...
Gene Information This gene is a member of the protocadherin gamma gene cluster one of three related clusters tandemly linked on chromosome five. These gene clusters have an immunoglobulin-like organization suggesting that a novel mechanism may be involved in their regulation and expression. The gamma gene cluster includes 22 genes divided into 3 subfamilies. Subfamily A contains 12 genes subfamily B contains 7 genes and 2 pseudogenes and the more distantly related subfamily C contains 3 genes. The tandem array of 22 large variable region exons are followed by a constant region containing 3 exons shared by all genes in the cluster. Each variable region exon encodes the extracellular region which includes 6 cadherin ectodomains and a transmembrane region. The constant region exons encode the common cytoplasmic region. These neural cadherin-like cell adhesion proteins most likely play a critical role in the establishment and function of specific cell-cell connections in the brain. Alternative ...
This gene is a member of the protocadherin gamma gene cluster, one of three related clusters tandemly linked on chromosome five. These gene clusters have an immunoglobulin-like organization, suggesting that a novel mechanism may be involved in their regulation and expression. The gamma gene cluster includes 22 genes divided into 3 subfamilies. Subfamily A contains 12 genes, subfamily B contains 7 genes and 2 pseudogenes, and the more distantly related subfamily C contains 3 genes. The tandem array of 22 large, variable region exons are followed by a constant region, containing 3 exons shared by all genes in the cluster. Each variable region exon encodes the extracellular region, which includes 6 cadherin ectodomains and a transmembrane region. The constant region exons encode the common cytoplasmic region. These neural cadherin-like cell adhesion proteins most likely play a critical role in the establishment and function of specific cell-cell connections in the brain. Alternative splicing has ...
This gene is a member of the protocadherin gamma gene cluster, one of three related clusters tandemly linked on chromosome five. These gene clusters have an immunoglobulin-like organization, suggesting that a novel mechanism may be involved in their regulation and expression. The gamma gene cluster includes 22 genes divided into 3 subfamilies. Subfamily A contains 12 genes, subfamily B contains 7 genes and 2 pseudogenes, and the more distantly related subfamily C contains 3 genes. The tandem array of 22 large, variable region exons are followed by a constant region, containing 3 exons shared by all genes in the cluster. Each variable region exon encodes the extracellular region, which includes 6 cadherin ectodomains and a transmembrane region. The constant region exons encode the common cytoplasmic region. These neural cadherin-like cell adhesion proteins most likely play a critical role in the establishment and function of specific cell-cell connections in the brain. Alternative splicing has ...
The basic structure of immunoglobulin (Ig) molecules is a tetramer of two light chains and two heavy chains linked by disulphide bonds. There are two types of light chains: kappa and lambda, each composed of a constant domain (CL) and a variable domain (VL). There are five types of heavy chains: alpha, delta, epsilon, gamma and mu, all consisting of a variable domain (VH) and three (in alpha, delta and gamma) or four (in epsilon and mu) constant domains (CH1 to CH4). Ig molecules are highly modular proteins, in which the variable and constant domains have clear, conserved sequence patterns. The domains in Ig and Ig-like molecules are grouped into four types: V-set (variable; IPR013106 ), C1-set (constant-1; IPR003597 ), C2-set (constant-2; IPR008424 ) and I-set (intermediate; IPR013098 ) [ (PUBMED:9417933) ]. Structural studies have shown that these domains share a common core Greek-key beta-sandwich structure, with the types differing in the number of strands in the beta-sheets as well as in ...
The basic structure of immunoglobulin (Ig) molecules is a tetramer of two light chains and two heavy chains linked by disulphide bonds. There are two types of light chains: kappa and lambda, each composed of a constant domain (CL) and a variable domain (VL). There are five types of heavy chains: alpha, delta, epsilon, gamma and mu, all consisting of a variable domain (VH) and three (in alpha, delta and gamma) or four (in epsilon and mu) constant domains (CH1 to CH4). Ig molecules are highly modular proteins, in which the variable and constant domains have clear, conserved sequence patterns. The domains in Ig and Ig-like molecules are grouped into four types: V-set (variable; IPR013106), C1-set (constant-1; IPR003597), C2-set (constant-2; IPR008424) and I-set (intermediate; IPR013098) [(PUBMED:9417933)]. Structural studies have shown that these domains share a common core Greek-key beta-sandwich structure, with the types differing in the number of strands in the beta-sheets as well as in their ...
中國醫藥大學新藥開發研究所 ∣ 台中市北區學士路91號 立夫大樓7樓 ∣ 04-22053366 分機8101 ∣ [email protected]. ...
HCAK 1, HCAK1, Ig kappa chain C region, IGKC, Immunoglobulin kappa constant, Immunoglobulin kappa constant region, Immunoglobulin kappa light chain, Kappa 1 immunoglobulin light chain, Km, MGC111575, MGC62011, MGC72072, MGC88770, MGC88771, MGC88809.
Graph comparing abundance of Magnesium transport protein CorA, transmembrane region across major taxonomic groups and individual genomes. Taxonomic distribution of Magnesium transport protein CorA, transmembrane region domains in all kingdoms.
Gentaur molecular products has all kinds of products like :search , AbD \ GOAT ANTI MOUSE LAMBDA LIGHT CHAIN FITC-POLYCLONAL ANTIBODY \ 106002 for more molecular products just contact us
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FIGURE 1. B cell development and allelic inclusion in 4E10 mice. (A and B) Knock-in mice carrying serologically distinguishable endogenous H and L chain constant regions were generated by breeding homozygous knock-in mice with IgHa/a;Cκh/h mice. Spleen B cells of the indicated genotypes were assessed by flow cytometry using Abs against IgMb and mouse Cκ, to detect the transgene H and L alleles, respectively, and Abs against IgMa and human Cκ were used to identify chains derived from endogenous genes. Data are representative of results obtained with 4-10 mice/group. (C) Reduced splenic B cell numbers in 4E10 knock-in mice. B220+CD19+ splenic B cells were enumerated from mice of the indicated genotypes. Each symbol represents an individual mouse. Horizontal lines show means. Data were from at least three independent experiments. (D) Analysis of BM B cell developmental fractions according to the staining scheme of Hardy et al. (28). Fractions represent developmental stages as follows: A-C, proB; ...
TY - JOUR. T1 - The V(H) and C(H) immunoglobulin genes of swine. T2 - Implications for repertoire development. AU - Butler, J. E.. AU - Sun, J.. AU - Kacskovics, I.. AU - Brown, W. R.. AU - Navarro, P.. PY - 1996/11/1. Y1 - 1996/11/1. N2 - Swine have the largest number of IgG subclass genes of all species so far studied but have a single gene for IgA which occurs in two allelic forms that differ in hinge length. Swine also have constant region genes for Cμ and Cε, but lack a gene homologous to that which encodes IgD in rodents and primates, despite the otherwise high degree of sequence similarity of all other swine C (H) genes with those of humans. Swine have , 20 V(H) genes, a single J(H) and perhaps a limited number of D(H) segments. Newborn piglets show preferential V(H) and D(H) usage and may use gene conversion as a mechanism for expanding their antibody repertoire. Despite the close similarity of their Ig gene sequences to humans, swine belong to the group of animals that includes ...
Human Kappa Light Chain, 1 ml. Antibody to the kappa light chain of immunoglobulin is reportedly useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkins lymphomas.
|strong|Mouse anti Human kappa light chain antibody, clone 4G7|/strong| recognizes a conformation-independent, constantly expressed epitope of the human immunoglobulin kappa chain.
Rhesus macaques represent a valuable model in biomedical research and in development of vaccines and therapeutics. Due to the lack of reagents, the general properties of IgG and corresponding cellular receptors (FcγR) in this species are poorly characterized. We engineered recombinant IgGs containing each of the four rhesus macaque heavy constant region (CH) subclasses. To define FcγRs that mediate IgGs, we identified and characterized three FcγR classes, and generated recombinant cDNA constructs. cDNA IgH constructs were created by fusing - by sequence overlap extension PCRs - a gene segment encoding the murine variable heavy domain specific for the hapten NIP, an established specificity system for assessing antibody effector functions, with rhesus macaque CH fragments. The complete IgH constructs were transfected into J558L cells, a murine IgH-lost myeloma cell line expressing anti-NIP light chain. Secretion of engineered IgGs was determined by ELISAs using NIP-BSA and anti-monkey IgG-specific
Description: Description of target: This gene is a member of the protocadherin gamma gene cluster, one of three related clusters tandemly linked on chromosome five. These gene clusters have an immunoglobulin-like organization, suggesting that a novel mechanism may be involved in their regulation and expression. The gamma gene cluster includes 22 genes divided into 3 subfamilies. Subfamily A contains 12 genes, subfamily B contains 7 genes and 2 pseudogenes, and the more distantly related subfamily C contains 3 genes. The tandem array of 22 large, variable region exons are followed by a constant region, containing 3 exons shared by all genes in the cluster. Each variable region exon encodes the extracellular region, which includes 6 cadherin ectodomains and a transmembrane region. The constant region exons encode the common cytoplasmic region. These neural cadherin-like cell adhesion proteins most likely play a critical role in the establishment and function of specific cell-cell connections in ...
FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene is a member of the protocadherin gamma gene cluster, one of three related clusters tandemly linked on chromosome five. These gene clusters have an immunoglobulin-like organization, suggesting that a novel mechanism may be involved in their regulation and expression. The gamma gene cluster includes 22 genes divided into 3 subfamilies. Subfamily A contains 12 genes, subfamily B contains 7 genes and 2 pseudogenes, and the more distantly related subfamily C contains 3 genes. The tandem array of 22 large, variable region exons are followed by a constant region, containing 3 exons shared by all genes in the cluster. Each variable region exon encodes the extracellular region, which includes 6 cadherin ectodomains and a transmembrane region. The constant region exons encode the common cytoplasmic region. These neural cadherin-like cell adhesion proteins most likely play a critical role in the ...
Rose, S M.; Kuehl, W M.; and Smith, G P., Cloned mpc 11 myeloma cells express two kappa genes. A gene for a complete light chain and a gene for a constant region polypeptide. (1977). Subject Strain Bibliography 1977. 131 ...
The constant regions are gBlocks® Gene Fragments and undergo size verification by capillary electrophoresis and sequence verification by mass spectrometry. The variable regions cannot currently receive complete analysis (using NGS, which would be needed for this analysis, would be cost prohibitive). We rely on a validated process that we have experimentally confirmed by NGS that ensures ,80% of DNA species are present in the final material shipped ...
The image presented describes the basic method for performing a SELEX or In vivo selection experiment using single stranded nucleic acids (RNA,ssDNA,XNA) that are chemically synthesizedan have a constant region (CR) and a random region. Having the CR allows later amplification using PCR. The first step is subjecting the population of single stranded nucleic acids to specific selective condition in which function is possible. Then a (2) diverse subset of the population will perform the desired function and will be then (3) PCR amplified to make double stranded nucleic acids with the use of the CR introduced previously. Therefore the selection can continue to a following round, while at the same time a sample is obtained and can be sequenced. [8] ...
The image presented describes the basic method for performing a SELEX or In vivo selection experiment using single stranded nucleic acids (RNA,ssDNA,XNA) that are chemically synthesizedan have a constant region (CR) and a random region. Having the CR allows later amplification using PCR. The first step is subjecting the population of single stranded nucleic acids to specific selective condition in which function is possible. Then a (2) diverse subset of the population will perform the desired function and will be then (3) PCR amplified to make double stranded nucleic acids with the use of the CR introduced previously. Therefore the selection can continue to a following round, while at the same time a sample is obtained and can be sequenced. [8] ...
F. Aguilar, U.R. Charrondiere, B. Dusemund, P. Galtier, J. Gilbert, D.M. Gott, S. Grilli, R. Guertler, G.E.N. Kass, J. Koenig, C. Lambré, J-C. Larsen, J-C. Leblanc, A. Mortensen, D. Parent-Massin, I. Pratt, I.M.C.M. Rietjens, I. Stankovic, P. Tobback, T. Verguieva, R.A. Woutersen ...
TY - JOUR. T1 - Introduction of lysine residues on the light chain constant domain improves the labelling properties of a recombinant Fab fragment. AU - Hemminki, Ari. AU - Hoffren, Anna-Marja. AU - Takkinen, Kristiina. AU - Vehniäinen, Markus. AU - Mäkinen, Maija-Liisa. AU - Pettersson, Kim. AU - Teleman, Olle. AU - Söderlund, Hans. AU - Teeri, Tuula. N1 - Project code: BEL2019. PY - 1995. Y1 - 1995. N2 - Europium chelates provide a non-radioactive alternative for sensitive labelling of antibodies for diagnostic immunoassays. Lysine residues at antibody surfaces are ready targets for labelling by an isothiocyanate derivative of the europium chelate (Eu3+). Here the labelling efficiency of a recombinant anti-human α-fetoprotein (hAFP) Fab fragment has been improved by increasing its lysine content by protein engineering. Molecular modelling was used to identify three light chain constant domain surface arginine residues, R154, R187 and R210, which were mutated to lysine residues. The ...
LILRA2 [ENSP00000251377]. Leukocyte immunoglobulin-like receptor, subfamily A (with TM domain), member 2; Part of the innate immune responses against microbial infection. Specifically recognizes a set of N-terminally truncated immunoglobulins that are produced via cleavage by proteases from a range of pathogenic bacteria and fungi, including L.pneumophila, M.hyorhinis, S.pneumoniae, S.aureus and C.albicans. Recognizes epitopes that are in part in the variable region of the immunoglobulin light chains, but requires also the constant region for signaling. Binds to a subset of cleaved IgM, IgG3 and IgG4 molecules, but does not bind cleaved IgA1. Binding of N-terminally truncated immunoglobulins mediates activation of neutrophils. In monocytes, activation leads to the release of CSF2, CF3, IL6, CXCL8 and CCL3 and down-regulates responses to bacterial lipopolysaccharide (LPS), possibly via down-regulation of TLR4 expression and reduced signaling via TLR4. In eosinophils, activation by ligand binding ...
human alpha 1-antitrypsin promoter, human immunoglobulin heavy chain genomic DNA, Simian virus 40 Large T antigen nuclear localization signal (NLS), phage P1 Cre recombinase, Simian virus 40 poly A ...
In oncology, immunoglobulins (Ig) are used for therapeutic treatment. There is a need in the pharmaceutical development and manufacturing to determine the antibody concentrations. Complete IgG antibodies can be determined using the IgG Bio HT test, detecting the highly conserved Fc part of the molecules. Nevertheless, a rising number of artificially designed Ig products consists of the target binding Fab part only, missing the Fc region. The Ig Fab Bio HT test detects a constant region of the Kappa Light Chain in the Ig Fab fragment. Therefore, this test is suited also for the determination of truncated Ig fragments ...
These results strongly indicate that the double-Tc/KO mice can be used to obtain antigen-specific hu-mAbs with various isotypes exhibiting desired effector functions. Successful expression of all four hγ subclasses represents an advantage of using hCF vectors to bypass cloning steps because some sequences within the constant region of human IgH locus was found to be unclonable by conventional cloning systems (6). V gene complexity is supposed to be essential for restoration of normal humoral immune response (5), which is important for the production of high affinity hu-mAbs against variety of antigens. Therefore, high affinities of the resultant hu-mAbs suggest that the authentic repertoire of fully human Igs was reconstituted in the double-Tc/KO mice. Although more detailed structural analysis of hCFs may be required to determine whether human Ig loci contained in the double-Tc/KO mice are completely intact, the data presented here and in our previous report (7) suggest that they include ...
The invention discloses a multi-well plate guide protector for single- and multi-well dispensing in a multi-well plate. The guide protector comprises a protecting cover having a height and dimension a
One of the five classes of immunoglobulins; it is present in small amounts in serum and is thought to function in certain allergic responses ...
Complete information for IGHG4 gene (Protein Coding), Immunoglobulin Heavy Constant Gamma 4 (G4m Marker), including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
Podle zákona o evidenci tržeb je prodávající povinen vystavit kupujícímu účtenku. Zároveň je povinen zaevidovat přijatou tržbu u správce daně online; v případě technického výpadku pak nejpozději do 48 hodin ...