TY - ABST. T1 - Batf3-dependent classical dendritic cells are required for mounting optimal rotavirus-specific IgA immune responses. AU - Nakawesi, Joy. AU - Hütter, Julia. AU - Lahl, Katharina. N1 - Conference code: 44. PY - 2017. Y1 - 2017. M3 - Conference abstract in journal. VL - 86. SP - 281. JO - Scandinavian Journal of Immunology. JF - Scandinavian Journal of Immunology. SN - 0300-9475. IS - 4. M1 - A-31356. T2 - 44th Annual Meeting of the Scandinavian Society of Immunology. Y2 - 17 October 2017 through 20 October 2017. ER - ...
Rabbit monoclonal antibody raised against native human IgA. Native purified IgA from human plasma. (MAB12813) - Products - Abnova
Elson, C O.; Heck, J A.; and Strober, W, "T-cell regulation of murine iga synthesis." (1979). Subject Strain Bibliography 1979. 2305 ...
The intestine is the largest lymphoid tissue in the body. One striking feature of intestinal immunity is its ability to generate great amounts of noninflammatory immunoglobulin A (IgA) antibodies that serve as the first line of defense against microorganisms. The basic map of IgA production includes induction of mucosal B cells in the Peyers patches, circulation through the bloodstream and homing to intestinal mucosa of IgA-commited plasma cells, and local antibody production for export across the intestinal membranes. Multiple cytokines, including TGF-{beta}, IL-10, IL-4, IL-5, and IL-6, are required to promote IgA class switching and terminal differentiation process of the B cells. Secreted IgA promotes immune exclusion by entrapping dietary antigens and microorganisms in the mucus and functions for neutralization of toxins and pathogenic microbes ...
Virus-like particles (VLPs) provide a well-established vaccine platform; however, the immunogenic properties acquired by VLP structure remain poorly understood. In this study, we showed that systemic vaccination with norovirus VLP recalls human IgA responses at higher magnitudes than IgG responses under a humanized mouse model that was established by introducing human PBMCs in severely immunodeficient mice. The recall responses elicited by VLP vaccines depended on VLP structure and the disruption of VLP attenuated recall responses, with a more profound reduction being observed in IgA responses. The IgA-focusing property was also conserved in a murine norovirus-primed model under which murine IgA responses were recalled in a manner dependent on VLP structure. Importantly, the VLP-driven IgA response preferentially targeted virus-neutralizing epitopes located in the receptor-binding domain. Consequently, VLP-driven IgA responses were qualitatively superior to IgG responses in terms of the ...
The intestine is the largest lymphoid tissue in the body. One striking feature of intestinal immunity is its ability to generate great amounts of noninflammatory immunoglobulin A (IgA) antibodies that serve as the first line of defense against microorganisms. The basic map of IgA production includes induction of mucosal B cells in the Peyers patches, circulation through the bloodstream and homing to intestinal mucosa of IgA-commited plasma cells, and local antibody production for export across the intestinal membranes. Multiple cytokines, including TGF-{beta}, IL-10, IL-4, IL-5, and IL-6, are required to promote IgA class switching and terminal differentiation process of the B cells. Secreted IgA promotes immune exclusion by entrapping dietary antigens and microorganisms in the mucus and functions for neutralization of toxins and pathogenic microbes ...
The intestine is the largest lymphoid tissue in the body. One striking feature of intestinal immunity is its ability to generate great amounts of noninflammatory immunoglobulin A (IgA) antibodies that serve as the first line of defense against microorganisms. The basic map of IgA production includes induction of mucosal B cells in the Peyers patches, circulation through the bloodstream and homing to intestinal mucosa of IgA-commited plasma cells, and local antibody production for export across the intestinal membranes. Multiple cytokines, including TGF-{beta}, IL-10, IL-4, IL-5, and IL-6, are required to promote IgA class switching and terminal differentiation process of the B cells. Secreted IgA promotes immune exclusion by entrapping dietary antigens and microorganisms in the mucus and functions for neutralization of toxins and pathogenic microbes ...
Product is the lyophilized powder of human myeloma IgA (kappa) and buffer salts. Human IgA (kappa) is purified from human myeloma serum using multi-step procedures which may include salt fractionation, gel filtration, ion-exchange chromatography and immunoabsorption.
IgA subclass antibodies have been reported in a few studies in patients with CAC. IgA antibodies and IgA1 antibodies were increased in comparison with the
We have evaluated a novel B-cell FluoroSpot assay for the analysis of antibody responses in healthy individuals vaccinated intramuscularly with Influenza A (H1N1) antigen (Pandemrix®, GlaxoSmithKline). Using the FluoroSpot assay and an ELISpot assay run in parallel for comparison, we measured the frequency of cells secreting antigen-specific as well as total IgG or IgA antibodies seven days post vaccination. The assays were based on high affinity monoclonal antibodies for capture and detection of human IgG and IgA. Whereas conventional ELISpot analyzes IgG- and IgA-secreting B cells separately, fluorescent detection enabled simultaneous enumeration of B cells secreting IgG or IgA in the same well. The FluoroSpot protocol was also simpler as the assay could be performed without the need for an amplifying detection step. While having all the advantages of a conventional ELISpot assay, including high sensitivity, robustness and ease of performance, the FluoroSpot assay adds further value in reducing costs
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A new study gives insight into the workings of the first vaccine ever reported to modestly prevent HIV infection in people. Most vaccines work by...
We are delighted to announce that one of our long-time clients, IGas Energy, moved to the cloud with Axis. They will now enjoy additional benefits, including database and application hosting, report server hosting, SQL server database administration, a back-up service, as well as upgrades and patches. ...
References for Abcams Anti-Human IgA antibody (FITC) (ab4219). Please let us know if you have used this product in your publication
The goal of this proposal is to elucidate the mechanisms by which the adjuvant GM-CSF enhances vaccine induced IgG and IgA responses against SIV. These studies...
So out of my 5 kids no one tested true positive as celiac. Here are the results all together: 10yo girl: Total IgA Ref Range 52-290 mg/dL ...
To test the hypothesis that IgA nephropathy can result from a mucosal immune response, mice were orally immunized with one of three protein antigens for 14 wk. Such mice exhibited an essentially pure mucosal antibody response characterized by specific IgA-producing plasma cells in exocrine sites and specific IgA antibodies in serum. Furthermore, 73% of immunized mice had IgA and 88% had immunogen deposited in the glomerular mesangium, and 64% of immunized mice examined ultrastructurally had electron-dense mesangial deposits. All three were present concurrently in 57% of the immunized mice. No differences in regard to IgG or IgM were observed between immunized and control mice for any of these parameters. Mucosal immunization therefore can result in a specific immune response that leads to mesangial deposition of immune complexes containing IgA antibody. In its fundamental features the experimental renal lesion resembles that seen in the human disease IgA nephropathy. ...
TY - JOUR. T1 - Primary IgA nephropathy in elderly patients. AU - Cheungpasitporn, Wisit. AU - Nasr, Samih H.. AU - Thongprayoon, Charat. AU - Mao, Michael A.. AU - Qian, Qi. PY - 2015/6/1. Y1 - 2015/6/1. N2 - Aim Data on clinicopathological features, treatment and outcomes of IgA nephropathy (IgAN) in elderly patients are limited. Methods Native kidney biopsies with a pathological diagnosis of IgAN (n = 1084) from Mayo Clinic Rochester in the years 1994-2013 were examined. After exclusion of the secondary IgAN, 45 elderly IgAN patients (age ≥65 years) were identified. One hundred sixty-two younger adults (age 18-64 years) with IgAN were randomly selected for comparison. Results Compared with younger adults, elderly patients showed a higher rate of chronic hypertension (62.2 vs 27.2%), higher pulse pressures (65 ± 17 vs 51 ± 15 mmHg), requiring greater number of antihypertensive medications (2.5 ± 1.2 vs 1.7 ± 0.7) and lower blood haemoglobin (11.1 ± 2.3 vs 12.7 ± 2.1 g/dL) at time of ...
TY - JOUR. T1 - Circulating CD89-IgA complex does not predict deterioration of kidney function in Korean patients with IgA nephropathy. AU - Jhee, Jong Hyun. AU - Kang, Hye Young. AU - Wu, Meiyan. AU - Nam, Bo Young. AU - Chang, Tae Ik. AU - Jung, Su Young. AU - Park, Seohyun. AU - Kim, Hyoungnae. AU - Yun, Hae Ryong. AU - Kee, Youn Kyung. AU - Yoon, Chang Yun. AU - Park, Jung Tak. AU - Yoo, Tae Hyun. AU - Kang, Shin Wook. AU - Han, Seung Hyeok. PY - 2017/11/27. Y1 - 2017/11/27. N2 - Soluble CD89 (sCD89)-IgA complex plays a key role in the pathogenesis of IgA nephropathy (IgAN). However, there is a lack of evidence supporting this complex as a good biomarker for disease progression. This study aimed to evaluate the usefulness of sCD89-IgA complex for risk stratification of IgAN. A total of 326 patients with biopsy-proven IgAN were included. sCD89-IgA complex was measured by sandwich-enzyme-linked immunosorbent assay. The study endpoints were a 30% decline in estimated glomerular filtration rate ...
Summary of Facts and Submissions. I. European patent 1 210 445, based on European patent application No. 00 958 787.4 and published as International patent application WO 01/19974, was granted with 16 claims. Claims 1 and 2 read as follows:. 1. A method of enhancing expression of a desired protein at mucosal effector sites, said method comprising placing the protein to be expressed under the control of a promoter having SEQ ID NO 2 or SEQ ID NO 3 or a fragment or variant having at least 60% homology or any of these which has promoter activity, and causing expression in mucosal cells.. 2. A construct comprising a promoter selected from the PphoP and PpagC or fragments or variants thereof which can act as promoters, operatively interconnected with a nucleic acid which encodes a protein, able to induce a protective immune response against an organism, in a mammal to which it is administered, wherein said construct contains no further elements of the phoP or pagC gene.. Claims 3 to 9 were ...
Calhoun, W.J.; Christman, J.W.; Ershler, W.B.; Graham, W.G.; Davis, G.S., 1986: Raised immunoglobulin concentrations in bronchoalveolar lavage fluid of healthy granite workers
The effect of rat immunoglobulins and immune complexes on the locomotor function of rat polymorphonuclear leukocytes (PMN) was investigated in vitro. Rat immunoglobulin G1 (IgG1), IgG2a, IgG2b, and IgA monoclonal antibodies specific for the dinitrophenyl hapten were used. Both monomeric and polymeric IgA showed chemotactic activity in a dose-dependent manner. IgG1 and IgG2b also induced a dose-dependent locomotor response of PMN, but the nature of the induced migration was chemokinetic (enhancing random migration). IgG2a was chemotactic and induced maximal migration at a relatively low concentration. IgG1- and IgG2b-immune complexes induced stronger migration than antibody alone; however, IgA- and IgG2a-immune complexes did not. IgA was shown to modify the chemotactic movement of PMN induced by N-formylmethionyl-leucyl-phenylalanine (FMLP). In the presence of both IgA and FMLP in the lower chamber, the migration towards suboptimal concentrations of FMLP was enhanced. By contrast, IgA in the ...
Human colostrum contains many bioactive factors that must promote the development of intestinal mucosal immunity in infants. Especially, the presence of certain cytokines such as transforming growth factor (TGF)-β or IL-10 has been of great interest for IgA production as a function of mucosal immune response. In the present study, we attempted to investigate whether unidentified factors inducing generation of IgA-producing cells from naive B cells might exist in colostrum. For this purpose, colostrum samples were directly added to a culture consisting of naive B cells and dendritic cells from cord blood and CD40 ligand-transfected L cells, comparing with recombinant IL-10 (rIL-10) and/or rTGF-β. It was noted that most colostrum samples alone were able to induce IgA-secreting cells at higher levels than rIL-10 and/or rTGF-β. IgA-inducing activity of colostrum was abolished by neither anti-neutralizing mAbs against IL-10 nor TGF-β, though partially by anti-IL-6 mAb. We prepared partially ...
Secretory IgA (SIgA) antibodies directed against the O-antigen (O-Ag) component of lipopolysaccharide are the primary determinants of mucosal immunity to enteric bacterial pathogens, such as Shigella flexneri and Salmonella enteric serovar Typhimurium. In this report, we demonstrate that monoclonal IgA Abs against the O-Ag are effective inhibitors of the bacterial type 3 secretion systems (T3SS) required for bacterial entry into intestinal epithelial cells. While these Abs did not affect bacterial viability, they did transiently de-energize both S. flexneri and S. Typhimurium, possibly accounting for the "paralysis" that has been observed in the case of S. Typhimurium, following Ab treatment. O-Ag specific IgA Abs also elicited long lasting ultrastructural changes in the bacterial outer membranes, suggesting that S. flexneri and S. Typhimurium made undergo specific adaptations in response to SIgA. These findings may have implications for understanding immune surveillance in the gut. We have ...
NEFECON is an add-on treatment to other medications for nephropathy symptoms and kidney function, including ACEI and/or ARBs. Rigorous blood pressure control will be achieved over a 6-month Run-in Phase in which ACEI and/or ARB will be dosed to target a blood pressure of ,130/80 mm Hg and UPCR ,0.5 g/g. Patients who complete the Run-in Phase, and despite optimized ACEI and/or ARB therapy, have a UPCR ≥0.5 g/g OR urine protein ≥0.75 g/24hr will be eligible for randomization and entry into the treatment phase of the trial. Patients will remain on their ACEI and/or ARB dosing regimen for the duration of the trial.. Patients entering the treatment phase will be administered NEFECON (8 mg/day OR 16 mg/day) OR placebo for a phase of 9 months. A 3-month follow-up phase will follow on from the treatment phase, of which the first 2 weeks will be used to taper the dose of those patients that received 16 mg/day dosing to 8 mg/day, with the placebo and 8 mg/day groups receiving placebo to retain ...
Shop IgA receptor ELISA Kit, Recombinant Protein and IgA receptor Antibody at MyBioSource. Custom ELISA Kit, Recombinant Protein and Antibody are available.
Our immune system produces a number of different immunoglobulin complexes to defense against infections, allergens, etc. Among these are IgA, IgG, IgM, and IgE. In case of IgA Nephropathy, something appears to go wrong with either the form of the IgA immune complex itself, or with their production and clearance within the body, or the both. As a result, the immune complex will deposit in the glomeruli and lead to damages to the renal functional cells and tissues ...
Diarrheal diseases represent a major health burden in developing countries. Parenteral immunization typically does not induce efficient protection against enteropathogens because it does not stimulate migration of immune cells to the gut. Retinoic acid (RA) is critical for gut immunity, inducing upregulation of gut-homing receptors on activated T cells. In this study, we have demonstrated that RA can redirect immune responses elicited by s.c. vaccination of mice from skin-draining inguinal LNs (ingLNs) to the gut. When present during priming, RA induced robust upregulation of gut-homing receptors in ingLNs, imprinting gut-homing capacity on T cells. Concurrently, RA triggered the generation of gut-tropic IgA+ plasma cells in ingLNs and raised the levels of antigen-specific IgA in the intestinal lumen and blood. RA applied s.c. in vivo induced autonomous RA production in ingLN DCs, further driving efficient induction of gut-homing molecules on effector cells. Importantly, RA-supplemented s.c. ...
Mosteller, L M.; Kiyono, H; Lentz, M R.; Eldrige, J H.; M, S; C; and Mcghee, J R., "T cell clones from murine peyers patches. Abstr." (1982). Subject Strain Bibliography 1982. 1977 ...
Researchers from the UCSD Superfund Research Center in an impressive collaborative study, have found that chronic liver inflammation can also promote cancer by suppressing immunosurveillance - a natural defense mechanism in which its thought the immune system suppresses cancer development.. The study, led by Drs. Michael Karin (Distinguished Professor of Pharmacology and Pathology) and Shabnam Shalapour (Assistant Professor of Pharmacology), have shown that chronic inflammation and fibrosis in humans and mice with non-alcoholic fatty liver disease is accompanied by accumulation of liver-resident immunoglobulin-A-producing (IgA+) cells.. Dr. Michael Karin indciated that "This study provides one of the strongest and most direct demonstrations that adaptive immunity actively prevents liver cancer.". The researchers found that NASH-associated mutations provoke the immune system, including cytotoxic T cells, to recognize and attack the newly emerging cancer cells. However, chronic liver inflammation ...
Hepatitis A virus (HAV) replicates in the liver, and is excreted from the body in feces. However, the mechanisms of HAV transport from hepatocytes to the gastrointestinal tract are poorly understood, mainly due to lack of suitable in vitro models. Here, we use a polarized hepatic cell line and in vivo models to demonstrate vectorial transport of HAV from hepatocytes into bile via the apical cell membrane. Although this transport is specific for HAV, the rate of fecal excretion in inefficient, accounting for less than 1% of input virus from the bloodstream per hour. However, we also found that the rate of HAV excretion was enhanced in the presence of HAV-specific IgA. Using mice lacking the polymeric IgA receptor (pIgR(-/-)), we show that a proportion of HAV:IgA complexes are transported via the pIgR demonstrating a role for specific antibody in pathogen excretion.
Data Availability StatementThe datasets used and/or analyzed during the current research are available through the corresponding writer on reasonable demand. the condition in Saham region where in fact the MAF study was conducted. Bloodstream examples were collected from farm animals and sera were screened in parallel for antibodies using different serological tests. Results Using the RBT test, phase 1 sera showed seropositivity in sheep at 2.6%, (95% CI: 0.5C13.5%), in camel (5.9%, 1.1C27.0%), but not in sera from goats and cattle (0%). Using I-ELISA, seropositivity in goat was 3.1% (0.6C15.8%), with no positive sheep and cattle. Using c-ELISA for camel we found a seropositivity of 5.9% (1.1C27.0%). Furthermore, CFT seropositivity in goats was 21.9% (CI: 11.3C38.9), cattle and sheep sera were negative and camel was 5.9% (1.1C27.0%). In phase 2, the seropositivity in goats was 1.9% (1.4C2.6%), sheep 4.5% (3.5C5.8%), cattle 1.1%, (0.5C2.3%) and camels 18.2% (5.1C47.7%), Phase 3 sera were ...
How to deal with the IgA Nephropathy relapse? What the lifespan of IgA Nephropathy patients? Start to find the information of IgA Nephropathy basics, symptoms, treatment, Diet﹠Fitness.
Background/aims: Although the serum IgA/C3 ratio is a good biomarker to predict the diagnosis, histological findings, and prognosis of IgA nephropathy (IgAN), the chronol..
The intestinal mucosa is constantly exposed to microbial and dietary antigens, all of which are considered to induce IgA-secreting plasma cells. Thus, the limited IgA repertoire diversity predicted in previous studies (Dunn-Walters et al., 1997, 2000; Holtmeier et al., 2000; Stoel et al., 2005; Yuvaraj et al., 2009) is difficult to reconcile with the broad range of intestinal antigens. In this study, we show that in fact each individual harbors a private polyclonal and highly diverse IgA repertoire. Because we analyzed switched Ig sequences, repertoire analyses performed in this study did not require sorting of plasma cells. Instead, RNA was isolated directly from intestinal tissue, and IgA-encoding transcripts were enriched during reverse transcription. This approach was equivalent to our results obtained when RNA was isolated out of sorted plasma cells. The IgA repertoire showed characteristics reminiscent of the T cell receptor repertoire of CD8+ memory cells (Naumov et al., 2003), i.e., the ...
IgA nephropathy refers to inflammation of the filtering units in kidneys due to IgA deposition in kidneys. The following are the frequently asked questions by the patients with IgA nephropathy.
Is IgA Nephropathy hereditary? Will it be passed down to the children. I am sure many adults with IgA Nephropathy concern about this topic, so here we will give an introduction about it. Is IgA Nephropathy hereditary? IgA Nephropathy is an
Pharmalink has stopped a Phase IIb trial of its primary IgA nephropathy treatment early after it met its primary endpoint in a planned interim analysis. The success makes Pharmalink a rare example of a private biotech with an unpartnered, Phase III-ready asset, characteristics that inevitably lead to talk of IPOs and deals.
Blog on IgA + IgG + IgM secondary antibody product: The IgA - IgG - IgM n/a (Catalog #MBS539207) is a Secondary Antibody produced from Goat and is intended f...
Enzyme immunoassay for the detection of IgA Rheumatoid Factor in human serum or plasma. SmartEIA kit is specifically designed for automated analysis using the Agility instrument.
An Examination of Procedure for Measuring Secretory IgA in Saliva and the Relationship Between Allergy and IgA Concentration in Students. (Kawasaki Medical Welfare Journal Vol.7,No.1,1997 Abstracts) (1998 ...
IgA nephropathy (IgAN) is well identified by dominant IgA glomerular deposits; however, this immunohistologic entity can be an asymptomatic chance finding or present with an extremely variable course. The variable clinical and histologic expressions are likely to be the result of genetic and environmental factors modulating common pathogenetic and progression mechanisms.
IgA nephropathy (Bergers Disease) is a condition in which a protein (immunoglobulin A) causes damage to the kidneys. The outcome is very variable.
IgA Nephropathy progresses slowly over time, and often presents no symptoms initially. Treatments relieve symptoms & complications without curing the
This assay is designed for the in-vitro measurement of specific IgA autoantibodies against tissue transglutaminase (tTG) present in human serum, as an...
EDITOR-Clinicians are natural bayesians when it comes to diagnosis.1 They have to be. The alternative approach might be to use the methods of classical hypothesis testing, but probably only once.. The Neyman-Pearson diagnosis of coeliac disease. (Assume that the sensitivity and specificity of transglutaminase IgA are both 95%.). Parent: Well doctor, have you got the result of the test yet?. Doctor: Yes I have. … ...
The City no longer operate immunisation clinics. To find out where your local immunisation clinics are now being held please visit the Department of Health website.
Your trusted lab partner for Immunoglobulin A (IgA) Low Range testing, Viracor Eurofins delivers your results faster, when it matters most.
Lower urinary-interleukin-1 receptor-antagonist excretion in IgA nephropathy than in Henoch-Schönlein nephritis.: Urinary IL-1ra levels in IgAN patients were lo
What is immunoglobulin? There are five types of immunoglobulin; IgG, IgA, IgM, IgD and IgE. Levels may be increased or decreased.