TY - JOUR. T1 - Comparison of three anti-coccidioides antibody enzyme immunoassay kits for the diagnosis of coccidioidomycosis. AU - Malo, Joshua. AU - Holbrook, Eric. AU - Zangeneh, Tirdad. AU - Strawter, Chris. AU - Oren, Eyal -. AU - Robey, Ian. AU - Erickson, Heidi. AU - Carranza-Chahal, Racquel. AU - Durkin, Michelle. AU - Thompson, Cindy. AU - Hoover, Susan E.. AU - Ampel, Neil M.. AU - Wheat, L. Joseph. AU - Knox, Kenneth S.. PY - 2020/8/1. Y1 - 2020/8/1. N2 - Coccidioidomycosis is a common cause of community-acquired pneumonia in endemic areas of the southwestern United States. Clinical presentations range from self-limited disease to severe, disseminated disease. As such, early and accurate diagnosis is essential to ensure appropriate treatment and monitoring. Currently available diagnostic testing has variable accuracy, particularly in certain patient populations, and new tests may offer improved accuracy for the diagnosis of coccidioidomycosis. Serum samples from patients with ...

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specificalPrinciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ALDH5A1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ALDH5A1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ALDH5A1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ALDH5A1 bound in the initial step. The color development is stopped and the intensity of the color is measured. ...

Associations between house dust-associated beta-(1,3)-glucan exposure and airway inflammatory reactions have been reported, while such exposures in early childhood have been suggested to protect against asthma and wheezing. Most epidemiological studies have used reservoir dust samples and an inhibition enzyme immunoassay (EIA) for beta-(1,3)-glucan exposure assessment. The objective of this ... read more study was to develop inexpensive but highly sensitive enzyme immunoassays to measure airborne beta-(1,3)-glucans in low-exposure environments, like homes. Specificities of available anti-beta-(1,3)-glucan antibodies were defined by direct and inhibition experiments. Three suitable antibody combinations were selected for sandwich EIAs. beta-(1,3)-Glucans in passive airborne dust collected with an electrostatic dust fall collector (EDC) and floor dust from seven homes were measured with the three EIAs. Floor dust samples were additionally analyzed in the inhibition EIA. The sandwich EIAs were ...

Results obtained with a recently introduced enzyme immunoassay system (EIA) for the detection of cryptococcal antigen (Meridian Diagnostics Inc) were compared with those obtained by a latex agglutination (LA) method (Immuno-Mycologics, Norman, Oklahoma, USA). Fifty four samples were examined. There was 92% agreement between the two methods. One false positive result was obtained with LA, and one sample was inevaluable. The EIA was rapid and simple to perform. There was some evidence that it gave fewer false positive reactions and improved the diagnosis of genuine early cases.. ...

Plasma exchange to remove HIT antibodies: dissociation between enzyme-immunoassay and platelet activation test reactivities Academic Article ...

specificalIntended Uses: This ADAM10 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human ADAM10. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay,,ADAM10 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-ADAM10 antibody and an ADAM10-HRP conjugate. The assay sample and buffer are incubated together with ADAM10-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ADAM10 concentration since ADAM10 from ...

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In a previous study employing conventional immunological marker analysis we found that 17% of high grade malignant lymphomas were devoid of cytoplasmic and membrane immunoglobulin and also sheep erythrocyte receptors. Cryostat sections from 24 of these cases (four of low grade and 20 of high grade malignancy) were stained with a panel of 30 monoclonal antibodies and six polyclonal antisera using a sensitive immunoperoxidase method. All tumours expressed the leucocyte common antigen (detected by monoclonal antibody 2D1) and all lacked epithelial cytokeratin (monoclonal antibody LE61), confirming their haematopoietic origin. All but one of the lymphomas expressed antigens characteristic of either B cells (17 cases) or T cells (six cases), while one case (morphologically a centroblastic lymphoma) had an unusual dual phenotype in which strong staining for T6 (marker of immature T cells) was associated with expression of the pan B lymphocyte antigens detectable with To15, anti-B1, anti-Leu12. This ...

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Purified viral lysates can be used in the manufacture of detection systems using solid-phase enzyme immunoassay, Western blotting, dot-blotting and related techniques. The material is valuable as an immunogen and for the purification of viral proteins.

Estrone EIA Reproductive Markers 011-CAN-E-420,The procedure follows the basic principle of a competitive enzyme immunoassay where there is competition between an unlabeled antigen and an labelled antigen bound to the limited binding sites of a specific antiserum . The amount of enzyme conjugate complex (HRP-avidin: biotin-labelled estrone) bou,medicine,medical supply,medical supplies,medical product

Hiramoto, R; Jurandowski, J; Bernecky, J; and Pressman, D, Immunohistochemical identification of tissue culture cells. (1961). Subject Strain Bibliography 1961. 1057 ...

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MeSH-minor] Adenocarcinoma / drug therapy. Adenocarcinoma / metabolism. Adenocarcinoma / secondary. Adenocarcinoma, Clear Cell / drug therapy. Adenocarcinoma, Clear Cell / metabolism. Adenocarcinoma, Clear Cell / secondary. Adenocarcinoma, Mucinous / drug therapy. Adenocarcinoma, Mucinous / metabolism. Adenocarcinoma, Mucinous / secondary. Adult. Aged. Angiotensin II Type 1 Receptor Blockers / pharmacology. Animals. Apoptosis / drug effects. Benzimidazoles / pharmacology. Blotting, Western. Carcinoma, Medullary / drug therapy. Carcinoma, Medullary / metabolism. Carcinoma, Medullary / secondary. Case-Control Studies. Cell Proliferation / drug effects. Disease Progression. Electrophoretic Mobility Shift Assay. Female. Humans. Immunoenzyme Techniques. Inhibitor of Apoptosis Proteins. Male. Mice. Mice, Inbred BALB C. Mice, Nude. Middle Aged. Peritoneal Neoplasms / drug therapy. Peritoneal Neoplasms / metabolism. Peritoneal Neoplasms / pathology. Receptor, Angiotensin, Type 1 / chemistry. Receptor, ...

in Clinical Cancer Research : An Official Journal of the American Association for Cancer Research (1997), 3(2), 227-31. Tumor samples obtained from 72 patients resected for non-small cell lung cancer were stained immunohistochemically using an immunoperoxidase method and the MLuC5 monoclonal antibody specific for the 67 ... [more ▼]. Tumor samples obtained from 72 patients resected for non-small cell lung cancer were stained immunohistochemically using an immunoperoxidase method and the MLuC5 monoclonal antibody specific for the 67-kDa laminin receptor. Sixty-one of 72 patients (84.7%) displayed a MLuC5-positive reaction, which was usually localized in both the inner surface of the plasmatic membranes and the cytoplasm of neoplastic cells. When we compared the laminin receptor expression with clinicopathological and biological parameters such as histotype, grading, T status, N status, ploidy, proliferative activity, vessel invasion, and p53 protein accumulation, the following results were ...

TY - JOUR. T1 - Fibrous papule. T2 - An immunohistochemical study with an antibody to S-100 protein. AU - Spiegel, Joan. AU - Nadji, Mehrdad. AU - Penneys, Neal S.. PY - 1983/1/1. Y1 - 1983/1/1. N2 - We studied twenty biopsies taken from fibrous papules which were located in the central part of the face. The lesions, which were characteristic histologically, did not contain S-100 protein within the stellate cells in the papillary dermis, nor was this substance found in mesenchymal cells with some features of nevus cells. In control studies, S-100 protein was found using an unlabeled antibody peroxidase-antiperoxidase technic within nevus cells composing junctional, intradermal, and compound nevi, Spitz nevi, and halo nevi. It therefore seems unlikely that fibrous papule represents a form of degenerated nevus as some investigators have proposed.. AB - We studied twenty biopsies taken from fibrous papules which were located in the central part of the face. The lesions, which were characteristic ...

This study aimed at evaluating in outpatients an algorithm for the laboratory diagnosis of Clostridioides (Clostridium) difficile infection (CDI), i.e., enzyme immunoassays (EIAs) detecting bacterial glutamate dehydrogenase (GDH) and toxin A/B, followed by polymerase chain reaction (PCR) analyses of samples with discordant EIA results.. In total, 9802 examinations of stool samples by GDH and toxin EIAs performed in 7263 outpatients and 488 inpatients were analyzed retrospectively. Samples with discordant EIA results had been tested by a commercially available PCR assay detecting genes of the C. difficile-specific triose phosphate isomerase (tpi) and toxin B (tcdB).. Concordant EIA results (686 C. difficile-positive, 8121 negative) were observed for 8807 (89.8%; 95% CI, 89.2-90.4%) samples. Of 958 samples with discordant EIA results, 895 were analyzed using PCR and 580 of 854 GDH-positive/borderline, toxin-negative samples (67.9%; 95% CI, 64.7-71.0%) were positive for tpi and tcdB, while 274 ...

primary intravaginal (IVAG) infection in genetically athymic (nude) mice. Nude (nu/nu) N: NIH(S) and Balb/c mice, as well as their euthymic counterparts were IVAG infected with 5 x 105 pfu of HSV-2. The progression of the infection was followed by HSV-2 immunolabeling using the peroxidase-antiperoxidase technique in tissue sections of the whole body, electron microscopy, and viremia titration at two different timepoints. 70% of athymic NIH mice, 30% of euthymic NIH mice, and 80% of both athymic and euthymic Balb/c mice developed acute vulvovaginitis and died between 8-10 days post-infection (pi). Viremia was not detected in either athymic or euthymic mice. HSV-2 replicated in the vulvovaginal, vesical and perianal epithelia, then progressed towards the central nervous system mainly along autonomic nerves and ganglia. HSV-2 antigens were not detected in liver, spleen, kidney, skin, heart, lung or bone marrow. The conclusion is that the T-cell immune response seems to limit the IVAG infection of ...

We have adapted the PCR-EIA for amplification and detection of an autolysin gene fragment of the pneumococcus. The assay was found to have a quantitative sensitivity for the detection of DNA equivalent to the amount of DNA from three organisms. It detected all tested serotypes of pneumococcus, whereas other streptococci, H. influenzae type b, and S. aureus yielded a negative result.. The qualitative sensitivity and specificity of the assay were estimated on the basis of the results of the assay for the positive controls (specimens for tests of sensitivity) and negative controls (specimens for tests of specificity) and the ability of the assay to detect additional cases of pneumococcal infection determined by the results of the assay with the culture-negative CSF specimens from patients with meningitis. Seven of the eight specimens that were culture or LA positive for pneumococcus were positive by PCR-EIA. The isolate from the only specimen that tested negative was the only one that showed ...

Its February and this patient appears to have influenza. I need a result right now. I know theres a rapid test available.. Anyone involved with infectious disease laboratory testing has heard the above refrain from physicians in the emergency room, intensive care unit, or infectious disease service. Assay manufacturers are constantly developing rapid diagnostic tests for a large variety of infectious diseases. However, an assays speed does not always correlate with an assays performance. Magauran et al,1 in this issue of Infectious Diseases in Clinical Practice, report their experience in using rapid assays for detecting influenza and respiratory syncytial virus (RSV) in their hands during a 2-year time frame. Their laboratory, at the Cleveland Clinic Foundation in Cleveland, Ohio, USA, instituted rapid enzyme immunoassay (EIA) testing for influenza and RSV in the fall of 2004. Specimens that tested negative by the rapid EIA procedures were then subjected to standard direct ...

Report No FR0444 P GALE, B HEGARTY, K WILSON AND C D WATTS APRIL 1994 SUMMARY I BENEFITS An up-to-date review is provided of commercially available kits and new developments for enzyme immunoassay of organics in potable and raw waters enabling Water Industry staff to make informed judgements on the utility of these simple, inexpensive procedures for their monitoring requirements. II OBJECTIVES To review the availability of enzyme immunoassay kits for analysis of pesticides and other substances of concern to the water industry. To evaluate the kits in relation to water industry requirements and conventional analytical methods. To stimulate the development of appropriate kits by discussion of the water industry s requirements with the leading enzyme immunoassay kit manufacturers. To report on the kits developed for the analysis of atrazine and uron pesticides. III REASONS Conventional methods for the analysis of pesticides and other organic chemicals in water often use techniques such as ...

The modified immunoenzymatic method for detection of antibody coated bacteria (IP ACB) was compared with immunofluorescence technique (IF ACB) in the diagnosis of urinary tract infection. For the study 100 patients were employed with significant and insignificant bacteriuria. It was found that 81% of the results obtained by IP ACB and IF ACB were identical, however the immunoenzymatic method was more sensitive than immunofluorescence. Moreover, the IP ACB technique is simpler, less time consuming and may be performed by using the ordinary optic microscope.

The symptoms mentioned above are the mild symptoms for COVID-19, which begins mild and gradually develops into something severe. Some recover from these symptoms without hospitalization. However, others become critically ill and have difficulty in breathing.. The most vulnerable people who can contract the disease are the elderly and people who have underlying medical problems. These medical problems include heart problems, lung problems, diabetes, and cancer. However, those with mild symptoms can transmit the disease to other people.. Coronavirus COVID-19 IgM ELISA Assay Kit. The enzyme-linked immunosorbent assay (ELISA) is a biochemistry assay that detects proteins, peptides, hormones, and antigens from test samples. Detection is achieved by assessing the conjugated enzyme activity. The most crucial element in detection is the antigen-antibody interaction. ELISA assay uses the microplate-based enzyme immunoassay technique.. The pathogenic strains developed by coronaviruses cause respiratory ...

In the present study, C. pneumoniae was detected in throat swab specimens from pediatric patients with lower respiratory tract infections by PCR-EIA and by isolation in cell culture. The organism was recovered in culture from only 1 of 368 specimens tested. The reasons for the low sensitivity of culture in this study are unknown. Sensitivity of cell culture depends on a number of factors, such as sufficient numbers of viable chlamydiae, collection method, transport and storage conditions of specimens, and choice of cell lines. We examined throat swab specimens, and it is possible that these samples did not include enough cells which harbored the organism. We cannot exclude the possibility that the isolation rate ofC. pneumoniae might have been higher with other types of specimens. Comparative studies on the relative efficacy of throat swab, nasopharyngeal swab, and sputum samples for recovery ofC. pneumoniae from culture revealed that positive results were most frequently obtained with sputum ...

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A single human tumor tissue with 5-10 m thickness is mounted on a positively charged glass slide. The slides are fixed and dehydrated with acetone for consistent results with in situ hybridization and immunohistochemistry. More tumors maybe available upon request ...

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The ViraChip® is an immunoblot based on an enzyme-immunoassay in a microarray format, carrying highly purified specific antigens bound to nitrocellulose at defined positions. It is suited for the qualitative detection of antibodies against specific antigens in human serum.. One microarray is fixed at the bottom of each well in a standard microtiter plate (MTP). The single breakable wells are stored in a holding frame with 96 positions. During the serum incubation step antigen-specific antibodies bind to the immobilised antigens, herein after referred to as spots, on the microarray. During the conjugate reaction, the AP-conjugate binds to the antigen-antibody complex. The alkaline phosphatase converts the chromogen/substrate and thus, stains the antigen-antibody complex on the microarray purple. The washing procedures following serum, conjugate and chromogen/substrate incubation steps remove unbound antibodies and reagents.. The control spots include serum controls, conjugate controls, ...

This antibody is designed to detect laminin using western blotting analysis under non-reducing and non-heating conditions and for histology on frozen tissue sections and paraffin embedded tissue sections.

This antibody is designed to detect laminin using western blotting analysis under non-reducing and non-heating conditions and for histology on frozen tissue sections and paraffin embedded tissue sections.

Author(s): Stern, Kalyn Michiko | Abstract: Drug sensitization is thought to arise through changes in transcription, modification of signaling, and synaptic transmission. A novel rat gene, mrt1, may contribute to this neuronal plasticity as it is upregulated during sensitization. Mrt1 contains a PX domain which classifies it as a sorting nexins- thus mrt1 is also called SNX27. In addition, SNX27 also has a PDZ domain which has been demonstrated to interact with and mediate GIRK channel trafficking. To explore this interaction and the role that SNX27 may play in sensitization it is necessary to localize SNX27 expression. Using a novel SNX27 antibody I had three aims for my Masters project. First, to characterize the specificity of the antibody. Second, to characterize SNX27 expression in the brain, particularly in the hippocampus. Third, to correlate this expression back to an interaction with GIRK channels. The SNX27 antibody demonstrated good specificity for SNX27 protein. SNX27 was found to be

Principal Investigator：KATO Ihachi, Project Period (FY)：1996 - 1997, Research Category：Grant-in-Aid for Scientific Research (B), Section：一般, Research Field：Conservative dentistry

The unlabeled antibody enzyme strategy of immunohistochemistry: preparation and properties of soluble antigen-antibody sophisticated (horseradish peroxidase-antihorseradish peroxidase) and its use in identification of spirochetes. Interleukin-10 (IL-10) impacts the growth and differentiation of many hemopoietic cells in vitro; notably, it is a potent suppressor of macrophage and T cell capabilities. In IL-10-deficient mice, generated by gene specializing in, […]. ...

An Autumn Peach Reviewing my past year with uterine (endometrial) cancer, I am grateful for and thank everyone for their support. Also wishing my sisters with all gynecologic and breast cancers the very best this fall season. October 10, 2013: A year ago today, my reading group met to discuss The Book Thief by Markus Zusak,…

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Enzyme immunoassays were developed using monoclonal antibodies raised against somatic (O), flagellar (H) and capsular (Vi) antigens of Salmonella typhi. The assay based on anti-O monoclonal antibodies could specifically detect S. typhi and soluble lipopolysaccharide (LPS) isolated from S. typhi. Anti-H MoAbs detected motile S. typhi and soluble flagellar antigen. Monoclonal antibodies against capsular polysaccharide could detect Vi-containing S. typhi as well as soluble Vi antigen. The three assays reported here detected S. typhi with 100% sensitivity in blood culture broths obtained from bacteriologically confirmed typhoid patients and were negative with blood specimens containing Salmonella senftenberg, E. coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Proteus mirabilis or Streptococcus (α-hemolytic) derived from patients with pyrexia. The assays, however, did not demonstrate the presence of soluble antigens in sera and urine samples obtained from typhoid patients. ...

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The enzyme-linked immunosorbent assay (ELISA) (/ɪˈlaɪzə/, /ˌiːˈlaɪzə/) is a test that uses antibodies and color change to identify a substance. ELISA is a popular format of wet-lab type analytic biochemistry assay that uses a solid-phase enzyme immunoassay (EIA) to detect the presence of a substance, usually an antigen, in a liquid sample or wet sample. The ELISA has been used as a diagnostic tool in medicine and plant pathology, as well as a quality-control check in various industries. Antigens from the sample are attached to a surface. Then, a further specific antibody is applied over the surface so it can bind to the antigen. This antibody is linked to an enzyme, and, in the final step, a substance containing the enzymes substrate is added. The subsequent reaction produces a detectable signal, most commonly a color change in the substrate. Performing an ELISA involves at least one antibody with specificity for a particular antigen. The sample with an unknown amount of antigen is ...

TY - JOUR. T1 - Standardization of an enzyme immunoassay for human antibody to Haemophilus ducreyi. AU - Desjardins, M. AU - Thompson, CE. AU - Filion, LG. AU - Ndinya-Achola, JO. AU - Plummer, FA. AU - Ronald, AR. AU - Piot, P. AU - Cameron, DW. PY - 1992. Y1 - 1992. KW - B780-tropical-medicine. KW - Bacteriology. KW - Haemophilus ducreyi. KW - Antibody detection. KW - Immunoenzyme techniques. KW - Immunoglobulin G. KW - Immunoglobulin M. KW - Screening. KW - STD. KW - Sexually transmitted diseases. KW - Laboratory techniques and procedures. M3 - A1: Web of Science-article. VL - 30. SP - 2019. EP - 2024. JO - Journal of Clinical Microbiology. JF - Journal of Clinical Microbiology. SN - 0095-1137. ER - ...

RIDASCREEN®FAST PSP SC [R1905] - Intended use: RIDASCREEN®FAST PSP SC is a competitive enzyme immunoassay for the quantitative analysis of Paralytic Shellfish Poison (PSP) and related algae toxins in mussels.General information: PSP is a potential neurotoxin that occurs in various algae (Gonyaulax-species). Over the food chain it could reach mussels and therefore, PSP is mainly

RIDASCREEN®FAST Zearalenon [R5502] - Intended use: RIDASCREEN®FAST Zearalenon is a competitive enzyme immunoassay for the quantitative analysis of zearalenone in cereals and feed. The test was validated with different matrices of cereals and composed feed.General information: The mycotoxin zearalenone is formed by fungi of the genus Fusarium. Zearalenone is a phytohormone which displays, apart

Veratox® for Neomycin is a competitive enzyme immunoassay for the quantitative analysis of Neomycin in muscle.. Veratox for Neomycin enables international and government regulatory agencies, food manufacturers and processors, as well as quality assurance organisations, to detect neomycin in various sample types and to satisfy customer concerns about food safety.. ...

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Hundreds of drawings and paintings by students at North Chicago Community Unit School District 187 are on display this month at Gurnee Mills.

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MeSH-minor] Adenocarcinoma, Mucinous / drug therapy. Adenocarcinoma, Mucinous / metabolism. Adenocarcinoma, Mucinous / pathology. Benzamides. Carboplatin / administration & dosage. Carcinoma, Papillary / drug therapy. Carcinoma, Papillary / metabolism. Carcinoma, Papillary / pathology. Cystadenocarcinoma, Serous / drug therapy. Cystadenocarcinoma, Serous / metabolism. Cystadenocarcinoma, Serous / pathology. Endometrial Neoplasms / drug therapy. Endometrial Neoplasms / metabolism. Endometrial Neoplasms / pathology. Female. Humans. Imatinib Mesylate. Immunoenzyme Techniques. Paclitaxel / administration & dosage. Piperazines / administration & dosage. Proto-Oncogene Proteins c-abl / genetics. Proto-Oncogene Proteins c-abl / metabolism. Proto-Oncogene Proteins c-kit / genetics. Proto-Oncogene Proteins c-kit / metabolism. Pyrimidines / administration & dosage. RNA, Messenger / genetics. RNA, Messenger / metabolism. RNA, Neoplasm / genetics. RNA, Neoplasm / metabolism. Tissue Array Analysis. Tumor ...

TY - JOUR. T1 - Immunohistochemical demonstration of a paracrine role of nitric oxide in bronchial function. AU - Rengasamy, A.. AU - Xue, C.. AU - Johns, R. A.. PY - 1994. Y1 - 1994. N2 - We addressed the controversial role of nitric oxide (NO) in bronchial function by an immunohistochemical study of the localization of NO synthase (NOS) and its effector protein, soluble guanylate cyclase, in rat bronchus. For this study, a monoclonal antibody to the bovine constitutive neuronal NOS was developed and characterized. In Western blot analysis, this monoclonal antibody (anti-NOS antibody) reacted with bovine cerebellum NOS (150 kDa) as well as with structurally different NOSs from cultured bovine aortic endothelial cells (130 kDa) and cultured RAW 264.7 macrophages (130 kDa). The reactivity of anti-NOS antibody was confirmed by immunohistochemical staining of rat cerebellum, arterial endothelial cells, and cultured stimulated macrophages. When the distribution of NOS in rat airway was ...

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As a qualitative serum with an optimized protein content to study the normal protein composition of bovine serum. To identify or quantitate a normal serum protein component using a variety of immunodiffusion techniques including immunoelectrophoresis, single and double radial immunodiffusion and electroimmunodiffusion. As a reference serum in nephelometry and other automated precipitation techniques. As a blocking agent or as a negative control in non-precipitating antibody-binding assays e.g. in serodiagnostic immunofluorescence and immunoenzyme tests. This normal reference serum does not contain assigned values for individual proteins but can be used as an internal relative standard for quantitative protein assays such as double radial immunodiffusion (Mancini, Fahey), ELISA, Western immunoblotting and electroimmunodiffusion (Laurell), expressing the results obtained with the serum sample under investigation as a percentage of the protein concentration in the normal reference serum ...

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Abstract: Arachidonic acid and PAF are released from the lungs during anaphyIaxis. The aim of this study is to describe the kinetics of the release of eicosanoids and to observe the effects of selected ihhibitors and a PAF antagonist on their release. Isolated lungs of previously sensitized animals were perfused via the pulmonary artery and challenged with ovalbumin. Prostaglandin E21 throraboxane B2, 6-keto prostaglandin F1? levels were determined in aliquots of effluents with radioimmunoassay and/or enzyme immunoassay techniques whereas leukotrienes B4 and D4 were measured by reverse-phase high performance liquid chromatography. In selected experiments 12-HHT and 12-keto HT were measured in the lung effluents. The peak release of eicosanoids from the lungs ensued approximately at 4-6 minutes after the initiation of anaphylactic shock. Perfusion of the lungs with aspirin, indomethacin decreased the formation of cyclooxygenase products and there was no significant change in the release of LTB4, ...

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Maturation of neurotransmission in the developing rat cochlea: immunohistochemical evidence from differential expression of synaptophysin and synaptobrevin 2

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Detection of pregnancy specific beta1-glycoprotein in formalin-fixed tissues.: Using an enzyme-bridge immunoperoxidase method, pregnancy specific beta1-glycopro

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Three of the 15 enzymes studied (EcoRII, NarI, and SgrAI) did not show complete cleavage even with the DNA relaxed, but there was still a clear drop in activity between the relaxed and stretched trials. In the cases of SgrAI and NarI the relative lack of activity may be attributable to the DNA template having relatively few recognition sites [only four and five sites, respectively, with minimum separations of 1,321 and 428 bp; looping of sites separated by ,1,000 bp is generally very slow (25)]. In the case of NarI this result is also consistent with the recent finding that, unlike most other type II REases, this enzyme only cleaves one strand of DNA per binding event and therefore has to bind twice to cleave dsDNA (26). Incomplete cleavage was also observed with EcoRII, for which the DNA template contained a similar number of sites and a similar distribution of separations as those of the enzymes for which complete cleavage was observed. Interestingly, this result might be explained by a recent ...

The DetectX® Triiodothyronine (T3) Enzyme Immunoassay kit is designed to quantitatively measure T3 present in urine, extracted serum and plasma, extracted dried fecal samples, and tissue culture media samples. This kit measures total T3 in extracted serum and plasma and in extracted fecal samples.. A T3 stock solution is provided to generate standard curves for the assay. Standards or diluted samples are pipetted into a coated microtiter plate. A T3-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of a sheep antibody to T3 to each well. After a two hour incubation the plate is washed and substrate is added. The substrate reacts with the bound T3-peroxidase conjugate. After a 30 minute incubation, the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader capable of measuring 450nm.. ...

De Gonozyme test (Abbott Laboratories), een nieuwe enzyme immunoassay (EIA) voor het aantonen van Neisseria gonorrhoeae werd geevalueerd in een grote groep promiscue vrouwen. Als de EIA werd uitgevoerd met materiaal afkomstig van de cervix, bedroeg de prevalentie van gonorroe 8,2%. Vergeleken met de resultaten, verkregen met de kweek, correspondeert deze waarde met een gevoeligheid van 88% en een specificiteit van 98.8%. Deze sensitiviteit is veel hoger dan de sensitiviteit gevonden van het grampreparaat (37%). Voor de urethra werden een sensitiviteit van 71% en een specificiteit van 99.0% gevonden. Bij een vergelijking van beide testen zonder een van beide als gouden standaard aan te nemen, bedroeg de gevoeligheid van de EIA van cervixmateriaal 89.9% en de specificiteit 99.0%. De EIA is bijzonder geschikt voor de snelle eliminatie van gonorroe uit de onderzochte patientenpopulatie. ...

Based on the versatile avidin-biotin complex method, the system is modular, and along with our selection of secondary antibodies, can accommodate a wide array of primary antibody and tissue species.

Recalls: Powdered Baby Formula - Similac. Parents who have purchased Abbott Laboratories product Similac as powdered baby formula are concerned following announcements yesterday and today related to a recall of 5,000,000 containers of Similac, the well-known powdered baby formula. The product is being recalled because it may contain insect parts of beetles and/or their larva. (So far, the type of beetle has not been made known.). Thus far, this recall does not affect any liquid baby formula manufactured by Abbott. Abbott Laboratories, based in North Chicago, Illinois, voluntarily closed the area of the Michigan processing plant where this particular product was made after discovering insects and have consulted the Food and Drug Administration, which deemed there are no immediately perceived deleterious effects on babies health, other than possible gastro-intestinal discomfort, which might discourage babies from eating.. The Similac products were sold in various sized plastic containers, ...

Figure 9. Inflammatory infiltrates in rejection associated arteritis are primarily T-cell infiltrates (Immunoperoxidase stain for CD3 antigen ...

HIT is an immune response to heparin that can result in thrombogenic thrombocytopenia. It is often a difficult diagnosis to make, and its diagnosis is dependent upon both clinical and serologic criteria. Due to this ambiguity, increased testing and treatment may occur until serologic test results return. Recently, scoring tools have been developed to assist with the diagnosis of this disease. However, these scoring tools have yet to be validated. Thus, we plan to conduct a prospective, observational study to validate two scoring tools, the 4Ts and the Chong scale, in the diagnosis of HIT.. In order to validate these scoring tools, two physicians will independently score the 4Ts and the Chong scale for all patients suspected of having HIT. These patients will be identified if a physician has ordered HIT antibody testing using the enzyme immunoassay (EIA) method. Confirmatory testing with a serotonin release assay (SRA) will also be performed. ...

The Biotin ELISA kit is an enzyme immunoassay used to determine the presence of Biotin (Vitamin H) in foods. View kit details and purchase online today.

Bioaim Mouse Gastrin EasyTest™ EIA Kit suitable for Plasma, Serum in mouse. Reliably quantify 18pg/ml of Gastrin. It takes 2.0 hours.

The News: Amgen Inc. (Thousand Oaks CA) has agreed to delay the US launch of its FDA-approved biosimilar version of AbbVie Inc.s (North Chicago) Humira (adalimumab) until 2023, under a… Read more ». ...

NORTH CHICAGO, Ill., March 14, 2017 /PRNewswire/ -- AbbVie Granted Priority Review in Japan for its Investigational Regimen of Glecaprevir/Pibrentasvir...

In this article, we demonstrate the use of a novel bioluminescent substrate for simple, multiwell plate-based CYP4F2 and CYP4F3 assays for rapid enzyme analyses and inhibitor screening applications.

I use Vector ABC kit routinely on perfused brains and it works very well. The few times I have tried to use it on fresh-frozen sections, the background was very high. Does anybody know why? Lilith ---------- From: Carol Burden To: [email protected] Subject: Re: [IHC on frozen sections of brain] Date: Wednesday, December 16, 1998 6:26PM [email protected] wrote: Hello Everyone, Does any of you have a tried and proven protocol for IHC on frozen brain sections using preferably the vector ABC-HRP system or any ABC staining system? your prompt reply will be greatly appreciated. Thanking you in advance. Atoska S.Gentry,B.S, HT(ASCP) Id like this info as well,could you please forward your findings. Sincerely Thanks,Carol at Emory ____________________________________________________________________ More than just email--Get your FREE Netscape WebMail account today at http://home.netscape.com/netcenter/mail ...

There are many types of EIA used in microbiology and biochemistry. Depending on the format of the test, some have alternative names such as ELISA and CLIA (chemoluminescent immunoassay). There is usually a solid phase, a surface or particle with an attached specific antibody or antigen. A patient sample is added; after time for binding,…

View the basic ABT stock chart on Yahoo Finance. Change the date range, chart type and compare Abbott Laboratories against other companies.

NEW YORK (MarketWatch) -- Abbott Laboratories undefined said Wednesday its fourth-quarter profit fell 35% to $1.05 billion, or 66 cents a share, from $1.62...

Rapid quantitation of biopharmaceutical products or contaminates from bioreactor/fermenters, downstream processing or final product quality contr

The upgrade to XPi with improved features such as AutoStart and the Auto Rack Loader clearly demonstrated that Siemens had thought deeply during the development stages about what would improve workflow and drive efficiencies for a time-pressured lab.. Mark ...

Hello, , , Im looking for murine anti-TNF and anti IL-1 mAbs to use on cryostat cut , mouse lung tissue sections. I know that such Abs are available for FACS , analysis and ELISA work, however I have failed to find any specifically , for immunohistochemistry. First of all, does it really matter? Can I use , Abs generally used in ELISAs or FACS analysis in immuno-histo? and , secondly does anyone know where to get such Abs, that have been proven to , work on tissue sections. , , , I look forward to your comments and help, , , , Dr. A. Kadioglu , , ak13 at le.ac.uk ...

Immunoperoxidase labelling of Liu-FPN1 in rat kidney. (A) Strong labelling is evident throughout the cortex and outer stripe of the outer medulla. Labelling is

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