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TY - CONF. T1 - Functional astrocyte-neuron lactate shuttle in a human stem cell derived neuronal network. AU - Hill, Eric. AU - Tarczyluk, Marta. N1 - Biotechnology and Biological Sciences Research Council [BB/H008527/1]; Alzheimers Research UK [PPG2009B-3]. PY - 2013. Y1 - 2013. N2 - The development of stem cell-derived neuronal networks will promote experimental system development for drug screening, toxicological testing and disease modelling, providing that they mirror closely the functional competencies of their in vivo counterparts. The NT2 cell line is one of the best documented embryocarcinoma cell lines, and can be differentiated into neurons and astrocytes. Great focus has also been placed on defining the electrophysiological properties of these cells, and more recently we have investigated the functional properties of their associated astrocytes. We now show for the first time in a human stem cell derived co-culture model that these cultures are also metabolically competent and ...

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Protein Involved In Synthesis Of The Thiamine Precursor HMP; Member Of A Subtelomeric Gene Family Including THI5, THI11, THI12, And THI13; Hydroxymethylpyrimidine Is Also Known As HMP

In these cyclic forms, the ring usually has 5 or 6 atoms. These forms are called furanoses and pyranoses, respectively - by analogy with furan and pyran, the simplest compounds with the same carbon-oxygen ring (although they lack the double bonds of these two molecules). For example, the aldohexose glucose may form a hemiacetal linkage between the hydroxyl on carbon 1 and the oxygen on carbon 4, yielding a molecule with a 5-membered ring, called glucofuranose. The same reaction can take place between carbons 1 and 5 to form a molecule with a 6-membered ring, called glucopyranose. Cyclic forms with a 7-atom ring called heptoses are rare.. Two monosaccharides can be joined together by a glycosidic or ether bond into a disaccharide through a dehydration reaction during which a molecule of water is released. The reverse reaction in which the glycosidic bond of a disaccharide is broken into two monosaccharides is termed hydrolysis. The best-known disaccharide is sucrose or ordinary sugar, which ...

TY - JOUR. T1 - Creation of a long-acting nanoformulated 29,39-dideoxy-39-thiacytidine. AU - Guo, Dongwei. AU - Zhou, Tian. AU - Araínga, Mariluz. AU - Palandri, Diana. AU - Gautam, Nagsen. AU - Bronich, Tatiana K. AU - Alnouti, Yazen. AU - McMillan, JoEllyn M. AU - Edagwa, Benson J. AU - Gendelman, Howard Eliot. PY - 2017/3/1. Y1 - 2017/3/1. N2 - Background: Antiretroviral drug discovery and formulation design will facilitate viral clearance in infectious reservoirs. Although progress has been realized for selected hydrophobic integrase and nonnucleoside reverse transcriptase inhibitors, limited success has been seen to date with hydrophilic nucleosides. To overcome these limitations, hydrophobic long-acting drug nanoparticles were created for the commonly used nucleoside reverse transcriptase inhibitor, lamivudine (29,39-dideoxy-39-thiacytidine, 3TC). Methods: A 2-step synthesis created a slow-release long-acting hydrophobic 3TC. Conjugation of 3TC to a fatty acid created a myristoylated ...

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Blabolil, P., M. Riha, D. Ricard, J. Peterka, M. Prchalova, M. Vasek, M. Cech, J. Frouzová, T. Juza, M. Muska, M. Tuser, V. Drastik, Z. Sajdlova, M. Šmejkal, L. Vejřík, J. Matena, David S. Boukal, D. Ritterbusch, and J. Kubečka. 2017. A simple fish-based approach to assess the ecological quality of freshwater reservoirs in Central Europe. Knowl. Manag. Aquat. Ecosyst. 418, doi:10.1051/kmae/ ...

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Are you being tempted by mulberries? Hey, whats in your mind now? Well, how about considering this very useful mulberry tree for the garden or on a commercial scale?

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Easy to grow, mulberries are care free plants that require minimal care. Give the long lived trees plenty of space. - Growing Mulberries - Landscaping at BellaOnline

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The importance of non-glucose carbohydrates, especially mannose and inositol, for normal development is increasingly recognized. Whether pregnancies complicated by abnormal glucose transfer to the fetus also affect the regulation of non-glucose carbohydrates is unknown. In pregnant sheep, maternal insulin infusions were used to reduce glucose supply to the fetus for both short (2-wk) and long (8-wk) durations to test the hypothesis that a maternal insulin infusion would suppress fetal mannose and inositol concentrations. We also used direct fetal insulin infusions (1-wk hyperinsulinemic-isoglycemic clamp) to determine the relative importance of fetal glucose and insulin for regulating non-glucose carbohydrates. A maternal insulin infusion resulted in lower maternal (50%, P | 0.01) and fetal (35-45%, P | 0.01) mannose concentrations, which were highly correlated (r2 = 0.69, P | 0.01). A fetal insulin infusion resulted in a 50% reduction of fetal mannose (P | 0.05). Neither maternal nor fetal plasma

Understanding brain energy metabolism-neuroenergetics-is becoming increasingly important as it can be identified repeatedly as the source of neurological perturbations. Within the scientific community we are seeing a shift in paradigms from the traditional neurocentric view to that of a more dynamic, integrated one where astrocytes are no longer considered as being just supportive, and activated microglia have a profound influence. Lactate is emerging as the good guy, contrasting its classical bad guy position in the now superseded medical literature. This review begins with the evolution of the concept of lactate shuttles; goes on to the recent shift in ideas regarding normal neuroenergetics (homeostasis)-specifically, the astrocyte-neuron lactate shuttle; and progresses to covering the metabolic implications whereby homeostasis is lost-a state of allostasis, and the function of microglia. The role of lactate, as a substrate and shuttle, is reviewed in light of allostatic stress, and ...

Deoxynojirimycin (DNJ) is the archetypal iminosugar, in which the configuration of the hydroxyl groups in the piperidine ring truly mimic those of d-glucopyranose; DNJ and derivatives have beneficial effects as therapeutic agents, such as anti-diabetic and antiviral agents, and pharmacological chaperones for genetic disorders, because they have been shown to inhibit α-glucosidases from various sources. However, attempts to design a better molecule based solely on structural similarity cannot produce selectivity between α-glucosidases that are localized in multiple organs and tissues, because the differences of each sugar-recognition site are very subtle. In this study, we provide the first example of a design strategy for selective lysosomal acid α-glucosidase (GAA) inhibitors focusing on the alkyl chain storage site. Our design of α-1-C-heptyl-1,4-dideoxy-1,4-imino-l-arabinitol (LAB) produced a potent inhibitor of the GAA, with an IC50 value of 0.44 µM. It displayed a remarkable ...

Astrocytes are now recognized as dynamic signaling elements in the brain. Bidirectional communication between neurons and astrocytes involves integration of neuronal inputs by astrocytes and release of gliotransmitters that modulate neuronal excitability and synaptic transmission. The ovarian steroid hormone, 17\beta-estradiol, in addition to its rapid actions on neuronal electrical activity can rapidly alter astrocyte intracellular calcium concentration $([Ca^{2+}]_i)$ through a membrane-associated estrogen receptor. Using calcium imaging and electrophysiological techniques, we investigated the functional consequences of acute treatment with estradiol on astrocyte-astrocyte and astrocyte-neuron communication in mixed hippocampal cultures. Mechanical stimulation of an astrocyte evoked a $[Ca^{2+}]_i$ rise in the stimulated astrocyte, which propagated to the surrounding astrocytes as a $[Ca^{2+}]_i$ wave. Following acute treatment with estradiol, the amplitude of the $([Ca^{2+}]_i)$ elevation in ...

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The fruitless mulberry is not a variety in itself; it is the male clone of any mulberry tree variety. The most common variety cloned is the white mulberry tree (Morus alba). The fruitless white mulberry tree is a tough, versatile tree often grown in hot inland areas. With wide leaves and a dense, spreading crown, ...

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Le Bagousse-Pinguet Y., Liancourt P., Götzenberger L., de Bello F., Altman J., Brozova V., Chlumska Z., Dvorsky M., Capkova K., Kopecky M., Rehakova K., Riha P., Leps J. & Dolezal J. 2018: A multi-scale approach reveals random phylogenetic patterns at the edge of vascular plant life. Perspectives in Plant Ecology, Evolution and Systematics 30, 22 - ...

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Fruitless mulberry trees are fast-growing and fairly large, providing dense shade and thick, lateral branches for climbing. But they are fairly disease-prone and also tend to have shallow roots, which can make them difficult to maintain. The best way to keep your fruitless mulberry happy and healthy in your yard is ...

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Mulberry is a deciduous tree that belongs to the fig family. There are 10 to 16 species of mulberry that can be found in warm, subtropical and temperate parts of the world. The best known and most commonly cultivated species are red, white and black mulbe

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Remember the mulberry cuttings I took back in late winter? A lot of them failed to take. They grew some lovely healthy looking green leaves but it was a trick - just the cutting drawing moisture up. When I checked, there was no real root development. But a decent number did take, and back in

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