Use of I region-restricted, antigen-specific T cell hybridomas to produce idiotypically specific anti-receptor antibodies. |...
Murine T cell hybridomas bearing receptors for antigen plus I region gene products were used as immunogens in mice in an effort to raise anti-receptor antisera. The antisera were assayed for anti-receptor activity by the ability to inhibit interleukin 2 production by the T cell hybridomas stimulated by antigen and I region expressing antigen-presenting cells. The T cell hybridomas used in these experiments were made by fusing antigen-specific, I region-restricted BALB/c T cell blasts to the AKR thymoma, BW5147. Three groups of mice were immunized with the T cell hybridomas: (BALB/c X AKR)F1 animals, syngeneic to the hybridoma; (BALB.B X aKR)F1 animals, differing from the hybridomas at H2; and (C.B20 X AKR)F1 animals, differing from the hybridomas at Igh. Mice were immunized multiple times and sera from individual animals were assayed for anti-receptor antibodies. In all groups, some mice produced anti-receptor antibodies by the criterion that they were inhibitory in the assay mentioned above. ...
Jens Christian Jensenius - Research outputs - Research - Aarhus University
Mannose-binding lectin (MBL) substitution: recovery of opsonic function in vivo lags behind MBL serum levels. / Brouwer, Nannette; Frakking, Florine N J; van de Wetering, Marianne D; van Houdt, Michel; Hart, Margreet; Budde, Ilona Kleine; Strengers, Paul F W; Laursen, Inga; Houen, Gunnar; Roos, Dirk; Jensenius, Jens C; Caron, Huib N; Dolman, Koert M; Kuijpers, Taco W.. In: Journal of Immunology, Vol. 183, No. 5, 2009, p. 3496-504.. Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review ...
Pharmaceuticals | Free Full-Text | Hybridoma-Derived Idiotype Vaccine for Lymphoma: Approval Must Wait | Notes
Hybridoma-derived idiotype vaccines have been used for the experimental treatment of human lymphoma over the last twenty years, providing evidence of biological efficacy, clinical efficacy and clinical benefit. However, the product that has come closer to regulatory approval is unlikely to clear that hurdle due to the insufficiently robust data obtained in a recently closed clinical trial. This review aims at discussing the reasons for hybridoma-derived idiotype vaccines, more difficult to produce but also more successful than recombinant idiotype vaccines so far, are unlikely to gain regulatory approval. In particular, it is necessary to examine the many peculiar features of this therapeutic approach in a broader context, with special attention to concepts like customized active immunotherapy and randomization. Most published trials based on hybridoma-derived idiotype vaccines are being analyzed, together with the yet non-peer reviewed data from the only randomized study conducted so far with this
Unscramble hybridomas | Words unscrambled from letters hybridomas | Scrabble Word hybridomas | Words Made with the Letters...
Unscramble hybridomas, Unscramble letters hybridomas, Point value for hybridomas, Word Decoder for hybridomas, Word generator using the letters hybridomas, Word Solver hybridomas, Possible Scrabble words with hybridomas, Anagram of hybridomas
Analysis of T-cell hybridomas with an unusual MHC class II-dependent ligand specificity<...
TY - JOUR. T1 - Analysis of T-cell hybridomas with an unusual MHC class II-dependent ligand specificity. AU - Mendiratta, S. K.. AU - Singh, Nagendra. AU - Bal, V.. AU - Rath, S.. PY - 1996/1/1. Y1 - 1996/1/1. N2 - We have characterized two unusual T-cell hybridomas, 1E3 and 3B8, from H-2(k) mice immunized with I-Ab-transfected L cells (H-2(k)), that are stimulated by L cells transfected with I-Ab, I-A(k) or I-Eb, but not by non-transfected L cells. These hybridomas could not be stimulated by spleen cells from H-2(i3), H-2(k), H-2b or H-2(d) mice. Monoclonal anti-I-A antibodies did not block their responses, suggesting that mouse major histocompatibility complex (MHC) class II molecules may be peptide donors rather than restriction elements for them. The stimulation of these hybridomas by fibroblast targets was not blocked by an anti-H-2k(k),D(k)-specific monoclonal antibody. Lipopolysaccharide (LPS)-activated splenic and peritoneal exudate cells from H-2(k), H-2(d), H-2(i3), H-2b as well as ...
Changes of monosaccharide availability of human hybridoma lead to alteration of biological properties of human monoclonal...
TY - JOUR. T1 - Changes of monosaccharide availability of human hybridoma lead to alteration of biological properties of human monoclonal antibody. AU - Tachibana, Hirofumi. AU - Taniguchi, Kiyotaka. AU - Ushio, Yoshitaka. AU - Teruya, Kiichiro. AU - Osada, Kazuhiro. AU - Murakami, Hiroki. PY - 1994/1/1. Y1 - 1994/1/1. N2 - The effect of glucose and other monosaccharide availability in culture medium on production of antibody by human hybridomas has been studied. Human hybridoma cells C5TN produce an anti lung cancer human monoclonal antibody, and the light chain is N-glycosylated at the variable region. When the cell line was grown in the presence of various concentrations of glucose, the antibodies produced changed their antigen-binding activities. Analysis of the light chains produced under these condition revealed that four molecular-mass variant light chains ranging from about 26 to 32 kDa were secreted. The twenty six-kDa species, which corresponds to a non-glycosylated form of the light ...
Equivalence of human and mouse CD4 in enhancing antigen responses by a mouse class II-restricted T cell hybridoma. | Journal of...
We have examined the ability of hCD4 to interact functionally with mouse class II MHC molecules using the mouse T cell hybridoma BI-141, specific for beef insulin. We have previously shown that expression of mouse CD4 results in a marked enhancement of IL-2 release by BI-141 cells in response to beef insulin or, in a cross-reactive response, to pork insulin, on the appropriate mouse APCs. We now demonstrate that expression of hCD4 results in an equivalent stimulation of antigen responses by this mouse T cell hybridoma. The specificity of this effect was demonstrated by mAb and gp120 blocking studies. These data provide the first direct evidence for function of hCD4 and in an exclusively mouse system. ...
A murine hybridoma with large cytoplasmic inclusions of kappa light chains. | JEM
A murine hybridoma cell line has been established that consistently forms large cytoplasmic inclusions. These structures bind antibody specific for mouse kappa L chain when stained in situ. SDS-PAGE analysis of isolated inclusion bodies produce a single protein band of approximately 26,000 Mr that reacts with anti-kappa antibody when transferred to nitrocellulose. No carbohydrate was detected in association with the purified protein. These data are consistent with the intracellular retention and deposition of complete kappa L chain protein. ...
Detection and characterization of murine ecotropic recombinant virus in myeloma and hybridoma cells<...
TY - JOUR. T1 - Detection and characterization of murine ecotropic recombinant virus in myeloma and hybridoma cells. AU - Deo, Y.. AU - Ghebremariam, H.. AU - Cloyd, M.. PY - 1994. Y1 - 1994. N2 - Ecotropic recombinant virus (ERV), a relatively new class of murine retrovirus endogenous to mice, is expressed at significant levels by most murine myeloma and hybridoma cells examined. The routine XC, S+L-, mink cell focus-inducing (MCF), and reverse transcriptase (RT) tests are not suitable to detect and quantify the levels of ERV. A serological focus assay, based on specific anti-murine leukemia virus (MuLV) viral envelope (env) antibodies, is required to detect ERV. A more sensitive format of this serological focus assay includes co-cultivation of test article cells with the indicator (Mus dunni) cells. ERV isolated from murine hybridoma cells show a unique pattern of cross-reactivity with anti-MuLV env antibodies and this pattern is clearly distinct from that of ectropic and xenotropic ...
Plus it
The bispecific monoclonal antibody (Bi-MAb) HRS-3/AP-1 was developed by somatic hybridization of the 2 mouse hybridoma cell lines HRS-3 and AP-1, which produce monoclonal antibodies with reactivity against the Hodgkins- and Reed-Sternberg cell-associated CD30 antigen and alkaline phosphatase, respectively. After an active incubation with alkaline phosphatase, purified whole immunoglobulin molecules and F(ab′)2 fragments of the Bi-MAb were equally effective in converting a relatively noncytotoxic prodrug, mitomycin phosphate (MOP), into mitomycin alcohol, which was 100 times more toxic to the Hodgkins- and Reed-Sternberg cell line L540 (CD30+) than MOP. The cytotoxic activity of MOP was unaffected when the cells were pretreated with either the Bi-MAb or the enzyme alone. The Bi-MAb HRS-3/AP-1 did not bind to HPB-ALL cells (CD30-) and was not able to activate MOP on these cells. In cocultivation experiments with HPB-ALL and L540 cells, the activation of MOP by the Bi-MAb HRS-3/AP-1 and ...
Expression and function of the transfected CD8 alpha chain in murine T cell hybridomas - GeoScience.net
Kanagawa, O.; Nakauchi, H.; Sekaly, R.P.; Maeda, K.; Takagaki, Y., 1990: Expression and function of the transfected CD8 alpha chain in murine T cell hybridomas
MHC-II antigen presentation by W cells is usually important in order | Ubiquitin-Mediated Proteolysis of the Neuronal and Tumor...
MHC-II antigen presentation by W cells is usually important in order for W cells to receive ideal costimulation from helper Compact disc4+ T cells. blend partner was created in the 1970s to generate B-cell hybridomas that secrete monoclonal antibodies (Kohler and Milstein, 1975). Thereafter Shortly, this technique was used to Capital t cells to create T-cell hybridomas that secrete IL-2 after TCR signaling (Kappler et al., 1982; Rock and roll et al., 1990). In light of the useful advantages of using peptide-specific T-cell hybridomas, researchers possess broadly used them as a device to quantitatively measure peptide-specific antigen demonstration by multiple types of antigen showing cells (APC). Vidovic et al exhibited that the adhesion substances and integrins in human being and murine Capital t cells are extremely extremely functionally conserved and murine T-cell:human being APC conversation happened easily (Vidovic et al., 2003). We and others possess utilized HLA-DR transgenic rodents to ...
Opti-Clone II - Hybridoma Cloning Factor (0910010) - MP Biomedicals
MPs Opti-Clone™ is a partially purified hybridoma growth medium supplement which improves the cloning efficiency of murine B-cell hybridomas. It will enhance the growth of hybridomas cultured at low cell densities, and it will dramatically increase the number of antibody producing colonies during HAT selection. It supports the growth of hybridomas used in the manufacture of monoclonal antibodies. The formulation is suitable for use in cloning and fusion applications. Features: ∙ Promotes hybridoma growth ∙ Eliminates feeder cell layers ∙ Increases antibody production ∙ Improves stressed cells viability ∙ Improves surviving hybridomas ...
Induction of c-ets and c-fos gene expression upon antigenic stimulation of a T cell hybridoma with inducible cytolytic capacity...
Expression of cellular oncogenes was studied in a T cell hybridoma that undergoes cytolytic activation when stimulated by specific antigen or by anti-Thy-1 antibody. The activation occurs without induction of hybridoma proliferation, providing a model to examine oncogene expression during functional differentiation of lymphocytes. We found that c-fos and c-ets-1 mRNAs were transiently induced at high levels in the hybridoma 30 min and 4 h after stimulation, respectively. c-myc and c-ets-2 oncogenes were constitutively expressed in the hybridoma and their mRNA levels were unaffected during 4 h of stimulation, although c-myc expression was reduced in the later stage of stimulation. Inhibitors of T cell activation, cyclosporin A and anti-LFA-1 antibody, blocked the induction of c-fos and c-ets-1 mRNAs without reducing the levels of c-myc and c-ets-2. The results indicate that the functional activation of the CTL hybridoma is associated with induction of c-fos and c-ets-1 genes. ...
New heteromyeloma cell lines for the production of human monoclonal antibodies. | OPENBS Open Archive UniBS
The aim of this study was to establish hybridomas capable of long-term production of human monoclonal antibodies (mAbs). Heterohybridization was performed between the mouse myeloma cell line P3X63Ag8.653 and activated human peripheral blood lymphocytes (PBL). In order to achieve better retention of human chromosomes, as well as to improve the stability of the heterohybrids, one HAT-sensitive immunoglobulin (Ig)-non-secreting human x mouse (h x m) heteromyeloma was fused for a second time with activated human PBL. In this way, a panel of HAT-sensitive Ig-non-secreting h x h x m heteromyelomas was obtained and tested for its ability to generate stable human Ig-secreting heterohybrids with activated human PBL. Six lines were selected on the basis of their enhanced characteristics of fusion efficiency and genetic stability. When fused with in vitro immunized human PBL, they generated several h x h x h x m hybridomas stably secreting high yields (10-23 micrograms/ml/24 h) of human mAbs reactive with ...
Transmural pressure induces IL-6 secretion by intestinal epithelial cells<...
TY - JOUR. T1 - Transmural pressure induces IL-6 secretion by intestinal epithelial cells. AU - Kishikawa, H.. AU - Miura, S.. AU - Yoshida, H.. AU - Hirokawa, M.. AU - Nakamizo, H.. AU - Higuchi, H.. AU - Adachi, M.. AU - Nakatsumi, R. C.. AU - Suzuki, H.. AU - Saito, H.. AU - Ishii, H.. N1 - Copyright: Copyright 2008 Elsevier B.V., All rights reserved.. PY - 2002. Y1 - 2002. N2 - Although intestinal epithelial cells (IECs) are known as an important source for IL-6, it is not known whether mechanical forces affect IL-6 production. We investigated how transmural pressure modulates IL-6 synthesis and activation of transcription factors in IECs. Pressure was loaded onto IEC-18 cells by introducing compressed helium gas into the cell culture flask for 1-48 h. IL-6 release into the culture media was determined by cell proliferation bioassay using an IL-6-dependent mouse hybridoma cell line (7TD1). Exposure to pressure (80mmHg) significantly enhanced IL-6 release into the culture media from IEC-18 ...
Primed B cells present type-II collagen to T cells.
article{9a5c9918-f28d-42f7-9e4a-e617d47af020, abstract = {Development of type-II collagen (CII)-induced arthritis (CIA) is dependent on a T-cell mediated activation of autoreactive B cells. However, it is still unclear if B cells can present CII to T cells. To investigate the role of B cells as antigen-presenting cells (APCs) for CII, we purified B cells from lymph nodes of immunized and nonimmunized mice. These B cells were used as APC for antigen-specific T-cell hybridomas. B cells from naïve mice did present native, triple-helical, CII (nCII) but also ovalbumin (OVA) and denatured CII (dCII) to antigen-specific T-cell hybridomas. In addition, B cells primed with nCII or OVA, but not dCII, activated the antigen-specific T-cell hybridomas two to three times better than naïve B cells. We conclude that antigen-primed B cells have the capacity to process and present CII to primed T cells, and antigen-primed antigen-specific B cells are more efficient as APC than naïve B cells. We further ...
Technology / Software - Hybridoma cell lines secreting monoclonal antibodies (Ref. TEC0072) - Content - Knowledge Portal (UPF)
XP Media and CloneMedia kits for Mouse Hybridoma Generation | Molecular devices
Read PDF to know how XP Media & CloneMedia for Mouse Hybridoma Generation provide a complete solution that supports all stages of hybridoma cell line development.
False-col SEM of hybridoma cell - Stock Image G400/0031 - Science Photo Library
False-colour scanning electron micrograph of a hybridoma cell producing a monoclonal antibody to cytoskeleton protein. Monoclonal antibodies are produced by injecting a mouse with an antigen & harvesting its antibody response by removing the B lymphocytes. These are fused with myeloma (tumour) cells taken from a mutant lymphocyte source. The fusion product is called a hybridoma cell. B lymphocytes are short-lived; when fused with tumour cells, which divide indefinitely, the continuous production of antibody is secured. Hybridomas are screened for the required antibody; they are then cloned & produce that antibody. Magnification: x1500 at 35mm size. - Stock Image G400/0031
Hybridomas
Hybridomas are immortalized cells derived from the fusion of B lymphoblasts with a myeloma fusion partner. Some hybridomas in the ATCC collection are somatic cell hybrids. These cells are capable of producing immunoglobulins that are specific for viral, bacterial or cellular targets.
Adaltis S.r.l. - Hybridomas (Cell Lines) & Monoclonal Antibody Production
Adaltis can produce purified and not purified antibody by using its cell lines. Antibodies can be purified on request by: HPLC , FPLC , affinity chromatography and gel filtration.. For production service information please contact: [email protected]. ...
COG3 - DNA Damage Signalling and Repair Inhibitors
The variable-region genes of monoclonal antibody against spores were cloned from mouse hybridoma cells by reverse transcription-PCR. food spoilage (9). Control of the bacterial spores in meals processing is vital that you ensure the basic safety and an extended shelf lifestyle of foods. To keep the product quality and basic safety of foods, polyclonal and […]. ...
Patent US6391637 - Use of CD40 ligand, a cytokine that binds CD40, to stimulate hybridoma cells - Google Patents
There is disclosed a polypeptide (CD40-L) and DNA sequences, vectors and transformed host cells useful in providing CD40-L polypeptides. More particularly, this invention provides isolated human and murine CD40-L polypeptides that bind to the extracellular binding region of a CD40 receptor. Also disclosed are methods of simulating hybridoma cells to increase monoclonal antibody production by administering a CD40 ligand polypeptide that stimulates B cell proliferation.
METHODS OF PRODUCING HYBRIDOMAS AND MONOCLONAL ANTIBODIES AND ANTIBODIES PRODUCED THEREBY - diagram, schematic, and image...
METHODS OF PRODUCING HYBRIDOMAS AND MONOCLONAL ANTIBODIES AND ANTIBODIES PRODUCED THEREBY - diagram, schematic, and image 150 ...
Patent US5700466 - Method of ameliorating or preventing septic shock using a monoclonal ... - Google Patents
The present invention pertains to the novel hybridoma SDW18.1.1, hybridomas obtained from SDW18.1.1, monoclonal antibodies obtained from such hybridomas and derivatives of such monoclonal antibodies. The novel hybridomas are formed by fusion of cells from a mouse myeloma line and spleen cells from a mouse previously immunized with cachectin/TNF. Diagnostic and therapeutic utilities for the monoclonal antibodies and their derivatives are proposed, and testing procedures, materials in kit form and pharmaceutical compositions are likewise set forth.
Anti-ABeta Globulomer Antibodies, Antigen-Binding Moieties Thereof, Corresponding Hybridomas, Nucleic Acids, Vectors, Host...
Anti-ABeta Globulomer Antibodies, Antigen-Binding Moieties Thereof, Corresponding Hybridomas, Nucleic Acids, Vectors, Host Cells, Methods of Producing Said Antibodies, Compositions Comprising Said Antibodies, Uses Of Said Antibodies And Methods Of Using Said Antibodies - diagram, schematic, and image 83 ...
DO11.10 TCR Antibody, anti-mouse - Hybridomas - MACS Antibodies - Products - Miltenyi Biotec - Danmark
The DO11.10 mouse model is a valuable tool for studies of T cell immigration, immunoregulation, development, activation, and function. The KJ1-26 monoclonal antibody reacts with the T cell receptor (TCR) expressed on lymphocytes of the DO11.10 transgenic mouse and the TCR of the BALB/c-derived DO11.10 and DO11.10.24 T cell hybridoma. The DO11.10 TCR is specific for the chicken ovalbumin (OVA) peptide (323-339) in the context of I-A[d] major histocompatibility (MHC) molecules. Transgenic DO11.10 T cells also recognize OVA peptide from jungle fowl and turkey in the presence of A20-1.11, the H-2d-bearing, antigen-presenting B cell lymphoma. - Danmark
Fibroblast blues
In a previous article, RAMPITSCH at BCRSSU.AGR.CA wrote: ,Hello nets: ,Im not sure if this is the right place to ask, but ..... ,I have a hybridoma cell line contaminated with fibroblasts. Normally I dont ,have a problem eliminating these since they stick to the plate a lot better ,than the hybridomas do: simply transferring the cells to a new plate usually ,does the trick. Now I have a line of fibroblasts which refuses to die (even ,after two months and more transfers than I care to remember). The hybridoma ,cell line (needless to say an important one) is growing poorly as it is barely ,able to attach to the plate and cant compete with these aggressive fibro- ,blasts (maybe they have been transformed?). Is there a miracle anti-fibro- ,blast agent? Is there any way of selectively getting rid of these fibroblasts? , ,Thanks for any help and comments, ,Chris R. , Chris: I may be brain-cramping but cant you just sort out the hybridomas? Or even pan them? Use something like an anti-Ig-FITC ...
Regulation of hybridoma antibody production by coculture with antigen by J J. Gibbons, Y T. Kim et al.
Gibbons, J J.; Kim, Y T.; and Siskind, G W., Regulation of hybridoma antibody production by coculture with antigen specific or idiotype specific immune spleen cell. Abstr. (1982). Subject Strain Bibliography 1982. 1805 ...
Kinetic characterization of metabolism and antibody production of a hybridoma cell line in high density homogeneous perfusion...
All rights reserved. No part of this book may be reproduced in any form by any electronic or mechanical means without permission in writing from the author ...
Growth limitation in hybridoma cell cultures: The role of inhibitory or toxic metabolites | SpringerLink
Hybridoma cells usually grow to fairly low cell densities in batch cultures (1-3×106 cells/ml). The reason for this is either that essential nutritional components of the medium are consumed, or that
KAKEN - Research Projects | INDUCTION OF HUMAN HEPATOMA-SPECIFIC IMMUNE RESPONSE BY HEPATOMA-DENDRITIC CELL HYBRIDOMAS (KAKENHI...
Principal Investigator:SHIMIZU Yukihiro, Project Period (FY):1999 - 2000, Research Category:Grant-in-Aid for Scientific Research (C), Section:一般, Research Field:Gastroenterology
Institut für Softwaretechnik und Theoretische Informatik: Multimodal Interaction
Topics In this course we will set the basics for an understanding of multimodal communication between humans and multimodal interaction between humans and machines. We will start with clarifying the basic principles of human-human communication and human-machine interaction. We will then describe the processes taking place in humans when perceiving auditory, visual and tactile signals, as well as how these perceptions are integrated in order to form a multimodal perception. The signals can be generated and received by machines which are able to interact with humans in limited domains. The set-up of such machines will be discussed, and limitations as well as potential solutions to overcome these limitations will be explained. The course is held in the MOOC format. Only the first session will take place in the lecture hall, where further information will be given and registration takes place. Being present in this session is mandatory for participating in the course. The remainder of the course ...
Institut für Softwaretechnik und Theoretische Informatik: Multimodal Interaction
Language: English VL Time: MOOC; New lecture available every week. VL Room: Online ISIS course: https://isis.tu-berlin.de/course/view.php?id=23345 Intro to the course and Q&A session in Zoom on April 19th, 2021 at 12:00-14:00. The link will be provided in the ISIS course. Please have a look into the intro video of the the course in ISIS and the structure of the course before the online session on April 19th. During this Zoom session we will introduce the general plan of the course and you can ask your questions about it. From 26th of April the lectures with chapters will be unlocked weekly, with homework and/or project tasks for each chapter. Topics In this course we will set the basics for an understanding of multimodal communication between humans and multimodal interaction between humans and machines. We will start with clarifying the basic principles of human-human communication and human-machine interaction. We will then describe the processes taking place in humans when perceiving ...
Corona Virus Disease (COVID-19)
1. It is the disease caused by the novel coronavirus. Patients may have fever with respiratory symptoms - cough, runny nose, sore throat or difficulty breathing. It can even be fatal if the symptoms are severe.. 2. Route of Transmission: Human-Human transmission through droplets from coughing and sneezing of infected person and close contact with COVID-19 cases.. 3. Incubation period: 2-14 days. ...
News | KurzweilAI
An international team has developed an AI algorithm with social skills that has outperformed humans in the ability to cooperate with people and machines in playing a variety of two-player games. The researchers, led by Iyad Rahwan, PhD, an MIT Associate Professor of Media Arts and Sciences, tested humans and the algorithm, called S# (S sharp), in three types of interactions: machine-machine, human-machine, and human-human. In most instances, machines programmed with… read more. ...
News | KurzweilAI
An international team has developed an AI algorithm with social skills that has outperformed humans in the ability to cooperate with people and machines in playing a variety of two-player games. The researchers, led by Iyad Rahwan, PhD, an MIT Associate Professor of Media Arts and Sciences, tested humans and the algorithm, called S# (S sharp), in three types of interactions: machine-machine, human-machine, and human-human. In most instances, machines programmed with… read more. ...
Join us for: Plantarium: Whats up at the Hub: Tema Genus: TEMA - Institutionen för Tema
The Gothenburg-based artist Nino Mick suggests in one of their poems that ultimately biology is our only home. The film programme suggests different ways of approaching such an understanding of biology as home. This film programme consists of a screening of two movies: Pojktanten and a short film/installation, Sporing Lips of Transposed Desire. Each of these two audio-visual artefacts explore floral-aesthetics through something that can be rendered as plant-human intimacies. The programme is inviting the audience as entrants - more than simply viewers - to engage in these ecological intimacies and to think of the ethics and potentials of thinking intimacies as beyond a human-human relationality. Approaching the films through such an angle of ecological intimacy might allow to perceive a decentred understanding of the human through multiplicities and entanglements with others. Wibke will offer in a short introductory talk to the programme as a thinking tool to apply to the film ...
Clinical Trials Registry | Internet Stroke Center
During the therapy session, visual 3D motion tracking will be used via Kinect, audio will be recorded, and basic measurements (i.e. the distance between a participants wrist and elbow) will be taken. After the patient-therapist interaction has been mapped, members of the research team will build computer based models of the therapist and patient using the insights gained from the human-human interaction. The models developed in this proposed study will be used as a template for programming safe and intuitive humanoid-patient interactions for future study. The model of the therapist will be implemented on the Robot (w/o a patient involved) and in a simulation environment where it will be tested with a computer-based model of the patient. ...
mouse anti-estrogen receptor alpha, ligand binding domain (aa 304-554) hybridoma (ERalpha BZ1) at eagle-i Network Shared...
mouse anti-estrogen receptor alpha, ligand binding domain (aa 304-554) hybridoma (ERalpha BZ1) is an eagle-i resource of type Hybridoma cell line at eagle-i Network Shared Resource Repository.
mouse anti-late bloomer hybridoma (10C9 anti-late bloomer) at eagle-i Network Shared Resource Repository
mouse anti-late bloomer hybridoma (10C9 anti-late bloomer) is an eagle-i resource of type Hybridoma cell line at eagle-i Network Shared Resource Repository.
Analysis of suppressor T cells induced by donor-specific transfusion (DST): establishment of a human T cell hybridoma producing...
TY - JOUR. T1 - Analysis of suppressor T cells induced by donor-specific transfusion (DST). T2 - establishment of a human T cell hybridoma producing an antigen-nonspecific suppressor factor.. AU - Fujiwara, T.. AU - Sakagami, K.. AU - Kusaka, S.. AU - Uda, M.. AU - Orita, K.. PY - 1992. Y1 - 1992. N2 - Formation of suppressor T cells (Ts) induced by donor-specific transfusion (DST) is one of the most commonly suggested mechanisms for the beneficial effect of DST. In this study, we established a human T cell hybridoma derived from the peripheral blood lymphocytes (PBL) of a DST-treated patient, which produced an antigen-nonspecific suppressor factor. Post-DST PBL were fused with an azaguanine-resistant mutant of a human T cell leukemia cell line, CCRF-CEM(AG). After selection and cloning, we established one clone producing the mixed lymphocyte reaction (MLR) inhibitory factor (C524: 18%-43% suppression). Suppressive activity of the supernatant obtained from C524 after activation by PHA was highly ...
Production of Monoclonal Antibody by Hybridoma Technology Introduction - Free Dissertation Writing For Students
Cesar Milstein and Georges J.F. Kohler invented the production of monoclonal antibodies in 1975 for which they got the Noble Prize of 1984 for Medicine and Physiology along with Niels Kaj Jerne, who made other contributions to immunology. The term hybridoma was introduced by Leonard Herzenberg in 1976-1977. Principle: The method starts by injecting a mice with immunogen. The mice should response to immunogenic reaction. A kind of somatic cell, the B-cell, produces antibodies that bind to the immunogen. These recently created antibodies are then collected from the mice. These isolated cells are merged with immortal B-cells (a myeloma cell), in order to provide a hybrid cell known as Hybridoma. These hybridomas have the antibody producing ability of the B-cell and also have the reproducibility and longevity. The hybridomas are fully grown in culture medium. In addition, the manufactured antibodies are all chemically identical in distinct to polyclonal antibody.. Hybridoma Production of Monoclonal ...
Guaranteed Mouse Monoclonal Antibody Production Services - Syd Labs
The most comprehensive custom mouse monoclonal antibody production services: custom production of monoclonal antibodies against non-modified, phospho-specific, methylation-specific, and acetylation-specific peptides and proteins; monoclonal antibodies for ELISA, Western blot, IP, IF, ICC, and IHC applications. Guaranteed ELISA > 1:40 000.
Monoclonal antibodies to various morphologic components of human skin<...
TY - JOUR. T1 - Monoclonal antibodies to various morphologic components of human skin. AU - Aiba, S.. AU - Masuko, T.. AU - Hosokawa, M.. AU - Hashimoto, Y.. PY - 1983/1/1. Y1 - 1983/1/1. N2 - Somatic cell hybrids were established from the mouse myeloma, P3x63Ag8.653 cells, and the spleen cells of a mouse hyperimmune to human epidermal cells. Indirect immunofluorescence test with hybridoma culture fluids displayed that 253 out of 263 hybridoma cultures secreted antibodies reactive with the frozen sections of human skin. The hybridomas secreting unique antibodies to skin components were cloned and designated as AHS-1 to -8. Monoclonal antibodies (MoAb) from AHS-1, AHS-2, and AHS-3 hybridomas did detect cytoplasmic antigens present in the epidermal layer, eccrine ducts and glands (except MoAb AHS-1), and hair follicles. Enzyme-linked immunosorbent assay showed that the antigen recognized by either MoAb AHS-1 or MoAb AHS-2, but not by MoAb AHS-3, shares the antigenic determinant with antigen(s) ...
HybriFree: a robust and rapid method for the development of monoclonal antibodies from different host species | BMC...
Monoclonal antibodies (MAbs) are made by identical immune cells and target one particular epitope by monovalent or monospecific affinity. The high affinity and selective binding of MAbs to epitopes in target antigens makes them highly potent tools for use in biochemistry, molecular biology and medicine. The first working method described for the isolation of monoclonal antibodies was hybridoma technology, based on forming hybrid cell lines (hybridomas) by fusing an antibody-producing B-cell with a myeloma cell [1]. The antibodies produced by a particular hybridoma clone share the same specificity. Thus, individual clones can be screened for the production of an antibody with the desired affinity. However, hybridoma technology has shortcomings: it takes a relatively long time (on the order of months) and has not been widely applied to organisms other than mice. Moreover, antibody sequence information is unavailable by this method. Thus, when a hybridoma-screened antibody is selected for further ...
Services Provided: Module 1 - NEI Vision Research Core
Our hybridoma bank contains over 20 murine hybridomas. Moreover, we have extensive experience in the purification and characterization of numerous murine monoclonal antibodies for in vitro and in vivo use. We have also generated monoclonal antibodies de novo by in vivo immunization and subsequent in vitro fusion of heterologous fusion partners from Chinese hamsters and rats, and produced IgA-secreting hybridomas de novo. Depending on the quantity of monoclonal antibodies required, hybridomas are propagated in either static flat tissue culture flasks or in roller bottle cultures. Monoclonal antibodies are purified by affinity column chromatography using protein G charged columns. The affinity purified monoclonal antibodies are dialyzed against deionized water, concentrated on a vacuum concentrator, resuspended in sterile PBS, and the protein concentration determined by BCA. The purity of the monoclonal antibodies is confirmed by western blotting with commercial anti-mouse IgG or IgM, antibodies, ...
Monoclonal Antibody Production | MD Anderson Cancer Center
The MD Anderson Monoclonal Antibody Core Facility provides custom monoclonal antibody production and purification to researchers at MD Anderson and beyond. Learn more.
Caco-2 Vs Hybridoma cells - Cell Biology
hi friends ! iam handling few cell lines ! we recently bought Caco-2 cell line. i have ended up mixing caco-2 cells with hybridoma cells and now iam unable to remove this contamination. Hybridoma cell line has a doubling time of just 24hrs is growing fast and soon will replace Caco-2 cells. caco-2 cells are really slow growing cells. i tried few mwthods to isolate them but failed ...
Commonalities Between pDCs and B Cells | Science Signaling
Plasmacytoid dendritic cells (pDCs) are a subpopulation of dendritic cells that secrete large amounts of interferons (IFNs) in response to stimuli that activate the Toll-like receptors TLR9 or TLR7. The C-type lectin blood dendritic cell antigen 2 (BDCA2) is uniquely expressed by pDCs, and antibodies that bind BDCA2 inhibit IFN production in response to TLR signaling. Cao et al. found that the abundance of BDCA2 at the cell surface was increased, and signaling was reconstituted in Jurkat cells or a mouse T cell hybridoma cell line when the cells were also transfected to express the γ subunit of the Fcε receptor (FcεR1γ) but not when BDCA2 was coexpressed with the immunoreceptor tyrosine-based activation motif (ITAM) adaptor DAP12 or the related protein DAP10. Analysis of the mRNA and protein abundance of signaling proteins associated with either B cell receptor (BCR) or T cell receptor (TCR) signaling revealed that the signaling cascade of isolated human pDCs most resembled that downstream ...
Monoclonal Ab
Monoclonal Antibody Production Service (Taiwan) Start Order Immediately by Completing the Inquiry Form.. I. Hybridoma fusion and screening service: 16 weeks (USD 5,100). II. MAP Immunogen monoclonal antibody production: Total 19 weeks (USD 5,800). III. LAE antigen monoclonal antibody production: Total 20 weeks (USD 6,600). IV. Optional Services. I Hybridoma fusion and screening service: 16 weeks. ...
Monoclonal antibodies that immunoreact with a cation-stimulated plant membrane ATPase | Biochemical Journal
Hybridoma technology has been used successfully to generate monoclonal-antibody probes against protoplast membrane antigens. Hybridomas secreting monoclonal antibodies that either inhibit or stimulate a putative plasma-membrane marker enzyme, (K+ + Mg2+)-stimulated pH 6.5 ATPase, have been identified and cloned. The specificity of monoclonal-antibody probes on the activity of other phosphate-hydrolysing enzymes has also been examined. The production and identification of monospecific antibodies capable of immunoreacting with particular component proteins in a complex plant membrane mixture highlight the usefulness of hybridoma methodology for the enzymologist, especially since such monoclonal antibodies can be used in the purification of proteins by immunoaffinity techniques. ...
Modified hybridoma methodology: Antigen-directed chemically mediated cell fusion<...
TY - JOUR. T1 - Modified hybridoma methodology. T2 - Antigen-directed chemically mediated cell fusion. AU - Kranz, David M. AU - Herron, James N.. AU - Billing, Patricia A.. AU - Voss, Edward W.. PY - 1980/1/1. Y1 - 1980/1/1. N2 - The concept of antigen-directed cell fusions to increase the yield of hybridomas was investigated. To facilitate cell-cell contact, antigen conjugated cells were used in cell fusion studies. Specifically, fluorescyl conjugated murine myeloma cells (Sp 2/0-Ag14) incubated with murine immune (anti-fluorescyl) splenocytes formed aggregates containing fluorescent and non-fluorescent cells. Fusion of these populations with polyethylene glycol resulted in a greater number of anti-fluorescyl hybridomas relative to normal fusions under non-antigen directed condition. Ligand binding data indicated that despite the multicellular aggregates the hybridomas resulting from chemically mediated fusions produced only one monoclonal Ig.. AB - The concept of antigen-directed cell fusions ...
Plus it
Within the functional group of transcriptional regulation, we find the transcription factor Fosl2 (also known as Fra2), a Fos family member, among the most prominently up-regulated by both chemopreventive compounds. Interestingly, an antitumor promoter, the phenolic antioxidant tert-butylhydroquinone, was reported to induce expression of Fra2 (Fosl2) as well as Fra1. Furthermore, the authors concluded that inhibitory activator protein-1 complexes composed of Jun-Fra heterodimers, induced by tert-butylhydroquinone, antagonize the transcriptional effects of the tumor promoter 12-O-tetradecanoylphorbol-13-acetate, which are mediated by Jun-Fos heterodimers (21). Similarly, inhibition of interleukin-6-stimulated cell growth of human myeloma and mouse hybridoma cells was shown to be associated with increased expression of Fra2 protein (22). Fra2 has also been associated with differentiation in epidermis, and exogenous expression of Fra-2 (Fosl2) repressed activator protein-1 transcriptional activity ...
Plus it
Recent isolates of RX54-3 hybridoma cells (new cells) protect BALB/c mice against subsequent challenge with the tumorigenic myeloma parent cells used to construct this hybridoma. In contrast, hybridoma cells which have been maintained in tissue culture for long periods of time (old cells) are not protective. In the present study, we compared a number of properties of the new and old hybridoma cells and determined which line was more similar to the parent myeloma. We found that new hybridoma cells resembled myeloma cells in: (a) possessing A- and C-type viral particles on transmission electron microscopy and a relatively smooth surface on scanning electron microscopy; (b) being sensitive to a hypotonic solution containing the dye propidium iodide; (c) having similar DNA histograms on flow cytometric analysis; (d) being sensitive to the bacteriocin colicin HSC 10; and (e) being tumorigenic in nude mice. In contrast, old hybridoma cells differed in all of these characteristics from new hybridoma ...
Hybridomas and monoclonal antibodies : presented at the Thirty-Fourth Annual Meeting of the American Association of Blood Banks...
Get this from a library! Hybridomas and monoclonal antibodies : presented at the Thirty-Fourth Annual Meeting of the American Association of Blood Banks, November 3, 1981. [Serafeim P Masouredis;]
Hybridoma culture using Eppendorf New Brunswick™ CelliGen® 310 with Packed-bed Fibra-Cel® Basket Impeller - Pharmaceutical...
receive-email Wed like to keep you informed about relevant promotions, products and services, if you would like not to receive these, check this box.. ...
Monoclonal Antibody Production - Sandwich ELISA guaranteed package - ProteoGenix
Premium package for production and purification of monoclonal antibodies guaranteed on sandwich ELISA. Discover also our list of available Packages online
Monoclonal Antibody Production And Purification
Production and Purification of your monoclonal antibodies (IgG, IgM and more) from Hybridoma or Cell Culture Supernatants produced by Davids or send to us.
Enhancement of hybridoma formation, clonability and cell proliferation in a nanoparticle-doped aqueous environment | BMC...
The isolation and production of human monoclonal antibodies is becoming an increasingly important pursuit as biopharmaceutical companies migrate their drug pipelines away from small organic molecules. As such, optimization of monoclonal antibody technologies is important, as this is becoming the new rate-limiting step for discovery and development of new pharmaceuticals. The major limitations of this system are the efficiency of isolating hybridoma clones, the process of stabilizing these clones and optimization of hybridoma cell secretion, especially for large-scale production. Many previous studies have demonstrated how perturbations in the aqueous environment can impact upon cell biology. In particular, radio frequency (RF) irradiation of solutions can have dramatic effects on behavior of solutions, cells and in particular membrane proteins, although this effect decays following removal of the RF. Recently, it was shown that nanoparticle doping of RF irradiated water (NPD water) produced a stabilized
Monoclonal Antibody Production - ELISA guaranteed package (Premium) - ProteoGenix
Premium package for production and purification of monoclonal antibodies guaranteed on ELISA system. Discover also our list of available Packages online
Epitopes of the cholera family of enterotoxins
Hybridoma-derived monoclonal antibodies were raised to enterotoxins of the cholera family and to chimeric B-subunit proteins in which individual amino acid residues of a heat-labile, cholera-related enterotoxin from an Escherichia coli strain of porcine origin (P-LT) were substituted with correspond …
Altweb: Publications: Proceedings: Alternatives in Monoclonal Antibody Production
September 24-25, 1997 , Baltimore, MD. A workshop of The Johns Hopkins Center for Alternatives to Animal Testing and The Office for Protection from Research Risks, National Institutes of Health ...
Monoclonal Antibody Production Service | Abbexa Ltd
At Abbexa, we have exciting offers and news about our products and services that we want to share with you. You can select if you would like to be contacted about specific topics below. We will always treat your data with care. We never share your details with any other companies.. If you change your mind later, you can stop receiving our updates at any time by changing your contact preferences in your account, or letting us know by email or phone.. ...
Generation of Anti-chemokine Monoclonal Antibodies with Migration Neutralizing Capacity: Their Potential as Therapeutic Agents...
Purpose: : Recruitment of immune cell subsets to the site of transplantation is potentiated in part by primordial chemokines such as CXCL1/KC and later CXCL9/Mig and CXCL10/IP-10, which are markedly up-regulated in high risk (HR) allogeneic corneal transplant recipients but not in syngeneic or normal risk allogeneic recipients. The purpose of this study is to address the effect of newly generated anti-chemokine mAbs as potential therapy for diminishing the influx of inflammatory immune cells into HR corneal allografts, therefore leading to enhanced graft survival. Methods: : Wild type (wt) C57BL/6 (B6), mice were immunized with CXCL1 peptide plus universal T cell epitope (PADRE) as adjuvant and boosted 14 days later with the same combination. Hybridoma lines were created by fusion of splenocytes from immunized mice with SP2/IL-6 myeloma cells. Anti-CXCL1/KC clones were selected based on their ability to bind whole CXCL1/KC by ELISA. Thioglycollate-elicited neutrophils or macrophages from ...
Hybridoma Hybridomas 4. Screening 5. Cloning and propagation - Free Critical Thinking For Students
An suitable adjuvant is blended with microgram or milligram amounts of immunogen and injected at multiple site of the mouse at various times in a repetitious manners. At times the mice is bled and examination for antibodies of purposed specificity is made. Highest concentration of antibodies are established after the mouse is immense with 2-3 individual delicate doses of antigen. At the point when concentration of antibody are most extreme the mouse is yielded and the spleen is taken. Then it is separated into individual spleenocyte by applying enzyme or instrumental process. Lymphocytes of the spleen is separated from rest of the organelles by Density gradient centrifugation . ...
IL-10 Antibody, anti-human | Hybridomas | MACS Antibodies | Products | Miltenyi Biotec | Canada
Interleukin 10 (IL‑10) is a cytokine predominantly secreted by CD4+ memory and effector T cells and antigen-presenting cells, for example, monocytes/macrophages and dendritic cells. IL-10 has important suppressive functions on immune responses and is believed to be involved in the maintenance of tolerance. IL-10 blocks activation of cytokine synthesis by Tʜ1 cells, activated monocytes, and NK cells. It can stimulate immunoglobulin production by B cells.The Anti-IL‑10 antibodies has been designed for intracellular staining of IL‑10-producing cells. Cells can be stimulated for IL‑10 production, for example, by polyclonal stimulation with mitogens. For induction of IL‑10 production by antigen-specific T cells, cells are re-stimulated with the respective antigen. IL‑10 can be accumulated in the cells by addition of secretion inhibitors like brefeldin A. After fixation and permeabilization of the cell sample, IL‑10-producing cells can be stained intracellularly with Anti-IL‑10 antibodies.
Positive Tested Species Reactivity: Drosophila-melanogaster, Insects, Clonality: Monoclonal
Hybridoma technology is used to fuse fusion a B cell and myeloma to form a hybridoma that produces identical monoclonal antibodies.
Positive Tested Species Reactivity: Wallaby, Clonality: Monoclonal
Hybridoma technology is used to fuse fusion a B cell and myeloma to form a hybridoma that produces identical monoclonal antibodies.