Periodical: McGee, Richard, Paul Simpson, Clifford Christian, Marina Mata, Phillip Nelson, and Marshall W. Nirenberg. Regulation of Acetylcholine Release from Neuroblastoma x Glioma Hybrid Cells. Proceedings of the National Academy of Sciences of the United States of America 75, 3 (March 1978): 1314-1318. Article. 5 Images ...
Hybrid (both intra-species and inter-species) cell lines arise through intentional or nonintentional fusion of somatic cells having different origins. Hybrid cell lines can pose a problem for authentication testing to confirm cell line identity, since the results obtained may not conform to the results expected for the two parental cell types. Thus, depending on the identity testing methodology, a hybrid cell may display characteristics of one of the parental cell type or of both. In some instances, the hybrid cell line may display characteristics that are different from those displayed by either parental cell type; these differences may not necessarily indicate cellular cross-contamination. Testing should be performed as soon as possible after an intended fusion has occurred, so that a baseline reference profile is available for later comparison. In this article, we describe the various approaches that have been used for identifying hybrid cell lines and the results that might be expected when using
Neuroblastoma x glioma hybrid NG108-15 cells endogenously express at least three receptors which activate adenylate cyclase via the intermediacy of the stimulatory G-protein, Gs. Sustained exposure of the cells to agonists at the IP prostanoid receptor results in a substantial decrease in cellular levels of the alpha-subunit of Gs (Gs alpha) [McKenzie and Milligan (1990) J. Biol. Chem. 265, 17084-17093; Adie, Mullaney, McKenzie and Milligan (1992) Biochem J. 285, 529-536]. By contrast, equivalent treatments of the cells with agonists at either the A2 adenosine receptor or the secretin receptor have no measurable effect on cellular amounts of Gs alpha. To examine whether this is a feature specific to the IP prostanoid receptor or is related to the level of expression of the individual receptors, NG108-15 cells were transfected with a construct containing a human beta 2-adrenoceptor cDNA under the control of the beta-actin promoter. Two clones of these cells were examined in detail, beta N22, ...
Cell Structure and Processes, Unit Membrane Chloroplast, Mitochondria, Rough Endoplasmic Reticulum, Smooth Endoplasmic Reticulum, Cell Membrane, Nucleus
Traber, J; Fischer, K; Latzin, S; and Hamprecht, B, "Morphine antagonizes the action of prostaglandin in neuroblastoma cells but not of prostaglandin and noradrenaline in glioma and glioma x fibroblast hybrid cells." (1974). Subject Strain Bibliography 1974. 2169 ...
Chromosomes were isolated from Chinese hamster x human hybrid cell lines containing four and nine human chromosomes. Human genomic DNA was biotinylated by nick translation and used to label the human chromosomes by in situ hybridization in suspension. Streptavidin was covalently coupled to the surface of magnetic beads and these were incubated with the hybridized chromosomes. The human chromosomes were bound to the magnetic beads through the strong biotin-streptavidin complex and then rapidly separated from nonlabeled Chinese hamster chromosomes by a simple permanent magnet. The hybridization was visualized by additional binding of avidin-FITC (fluorescein) to the unoccupied biotinylated human DNA bound to the human chromosomes. After magnetic separation, up to 98% of the individual chromosomes attached to magnetic beads were classified as human chromosomes by fluorescence microscopy. ...
Microinjection of DNA. Injection of linear DNA molecules into fertilized eggs (pronuclear stage) using an inverted microscope, micromanipulation equipment and injection / holding devices.. The first successful production of transgenic mice using pronuclear microinjection was reported in 1980 [1]. The pronuclear microinjection method of producing a transgenic animal results in the introduction of linear DNA sequences into the chromosomes of the fertilized eggs. If this transferred genetic material is integrated into one of the embryonic chromosomes, the animal will be born with a copy of this new information in every cell. The foreign DNA must be integrated into the genome prior to the doubling of the genetic material that precedes the first cleavage.. If this does not occur, only a few cells will integrate the gene. For this reason, the DNA is introduced into the fertilized egg at the earliest stage, which is the pronuclear period immediately following fertilization. For several hours following ...
Small cell lung cancer (SCLC) is known to express the HuD protein, the neuronal antigen homologous to Drosophila Elav and Sxl genes involved in neuronal and sex development. HuD is the target of an immune response including high titered antibodies causing paraneoplastic encephalomyelitis and sensory neuropathy. Because the p53 recessive oncogene is mutated and anti-p53 antibodies frequently occur in cancer patients, we wondered if the development of anti-HuD antibodies signaled the presence of HuD mutations in lung cancer. The HuD gene was mapped to chromosome region 1p using a human-mouse hybrid cell panel. We confirmed that 26 of 46 cancer (43 lung cancer and 3 mesothelioma) cell lines expressed HuD mRNA and that this expression, as well as protein expression by Western blot, correlated strongly with the SCLC neuroendocrine phenotype. Southern blot and single-strand conformation polymorphism analyses showed that HuD was not mutated in 78 lung cancers, including patients with the severe ...
Mutations in mitochondrial DNA (mtDNA) can be specifically targeted and removed by transcription activator-like effector nucleases (TALENs) in murine oocytes, single-celled mouse embryos, and fused human-mouse hybrid cells, providing proof of principle for a method that could one day be used to treat certain hereditary mitochondrial disorders in people. Between 1,000 and 100,000 mitochondria power each human cell. Often, mitochondria in the same cell have different genomes, or haplotypes, a condition known as heteroplasmy. Certain haplotypes include mutations that impact mitochondrial function and cause disease, particularly in energy-hungry organs such as the brain and heart. Because mitochondria segregate randomly as cells divide, it is impossible to determine early in embryonic development how a mix of wild-type and mutated mitochondria inherited from the mother will affect an organism.. To rid mitochondria of these harmful mutations, researchers have used restriction enzymes as well as ...
Systematic forward genetics using somatic cell mutants is a powerful tool in elucidating biochemical pathways [1]. However, it is often difficult to identify the genetic loci responsible for a particular somatic cell mutant phenotype. Map-based positional cloning strategies can be used in somatic cell hybrids to analyze mutants with recessive phenotypes. By tracking DNA sequence variants that co-segregate with heritable traits, the genes accounting for these traits can be localized to specific chromosomal locations [2]. Recent advances in genome sequencing have facilitated the assembly of physical maps of candidate regions, but fine mapping and narrowing of candidate regions is still often tedious and remains a major obstacle in somatic cell genetics. Consequently, there is a need to efficiently map the positions of genes in somatic cell mutants.. We previously established a method to enrich for human genes that complement rodent somatic cell mutants [3]. The mutant rodent cells are fused with ...
Ricciuti, F and Ruddle, F H., "Biochemical and cytological evidence for triple hybrid cell line formed from fusion of three different cells." (1971). Subject Strain Bibliography 1971. 330 ...
Gene expression profiling, chromosome assignment and mutational analysis of the porcine Golgi-resident UDP-N-Acetylglucosamine transporter SLC35A3 ...
A strategy was developed to generate expressed sequence tags (ESTs) from human pancreatic islet gene products using differential display of mRNA. Screening of over 2,000 cDNA amplification products identified 42 cDNAs that were preferentially expressed in pancreatic islets relative to exocrine tissue. Public database analysis showed that 29 (69%) corresponded to novel genes, in contrast with only 66 of 250 (26.4%) cDNA clones randomly selected from a human islet library. Reverse transcription-polymerase chain reaction (RTPCR) and/or Northern analysis of RNA from multiple tissues confirmed that expression was enhanced in human islet cell RNA for 11 of 15 tested cDNAs. Sequencetagged sites developed from 19 islet cDNAs were used to map these genes to human chromosomes using a combination of monochromosomal somatic-cell hybrids, genome-wide radiation hybrids, and mega-yeast artificial chromosome analysis. These results indicate that this PCR-based cDNA selection strategy yields information on a ...
But these Enteties are not changing but transmutating our so called DNA/RNA of our Cell-Organanelles (a kind of Cell-PhOne & Cell-PhoTones = SelfOnes & Selfie-tOnes through Light-&Sound-Interference-Frequencies of the Dia-Bolus-Tri-Tonus of Atomic-Harmony. And we have to respond to be open hearted enough to embrace our own channels of remembering, who & what we are if…
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Will it work? I am sceptical. This technology uses similar nuclear transfer techniques to those used in therapeutic cloning for embryonic stem cells (which has thus far failed to deliver) and animal-human cytoplasmic hybrids (cybrids). The wild claims made about the therapeutic properties of cybrids by the biotechnology industry, research scientists, patient interest groups and science journalists duped parliament into legalising and licensing animal human hybrid research in 2008. Few now will remember Gordon Browns empty promises in the Guardian on 18 May that year of cybrids offering a profound opportunity to save and transform millions of lives and his commitment to this research as an inherently moral endeavour that can save and improve the lives of thousands and over time millions of people. That measure was supported in a heavily whipped vote as part of the Human Fertilisation and Embryology Bill, now the HFE Act. But cybrids are now a farcical footnote in history. They ...
Will it work? I am sceptical. This technology uses similar nuclear transfer techniques to those used in therapeutic cloning for embryonic stem cells (which has thus far failed to deliver) and animal-human cytoplasmic hybrids (cybrids). The wild claims made about the therapeutic properties of cybrids by the biotechnology industry, research scientists, patient interest groups and science journalists duped parliament into legalising and licensing animal human hybrid research in 2008. Few now will remember Gordon Browns empty promises in the Guardian on 18 May that year of cybrids offering a profound opportunity to save and transform millions of lives and his commitment to this research as an inherently moral endeavour that can save and improve the lives of thousands and over time millions of people. That measure was supported in a heavily whipped vote as part of the Human Fertilisation and Embryology Bill, now the HFE Act. But cybrids are now a farcical footnote in history. They ...
Malignant tumours are the second main cause of mortality worldwide, with haematological malignancies representing 10%. Current treatment strategies, such as chemotherapy, radiotherapy, and stem cell transplantation, are mostly effective but may induce serious side effects. In addition, tumours are developing resistance against most of these conventional therapies. Immunotherapy is a promising investigational approach, especially hybrid cell vaccination, in which a professional APC is fused to a tumour cell, and the fusion product mostly combines the antigenicity of the tumour cell partner, processed and presented through the relevant APC-machinery, and associated with co-stimulatory molecules CD80, CD86, and CD40 expression, for proper induction of anti-tumour immune responses. I investigated the phenotypic and functional characteristics of a group of previously-made hybrid cell lines, generated by in vitro fusion of a human APC (HMy2; EBV B-lymphoblastoid cell line) with haematological ex vivo ...
The p27Kip1 gene codes for a cyclin-dependent kinase inhibitor implicated in G1 arrest by transforming growth factor β, cell-cell contact, agents that elevate cyclic AMP, and the growth-inhibitory drug rapamycin. p27 binds to and inhibits complexes formed by cyclin E-cdk2, cyclin A-cdk2, and cyclin D-cdk4. The involvement of p27 in the negative regulation of cell proliferation suggests that it may also function as a tumor suppressor gene. Using a combination of somatic cell hybrid panels and fluorescence in situ hybridization p27Kip1 has been mapped to the short arm of chromosome 12 at the 12p12-12p13.1 boundary, reported to harbor deletions and rearrangements in leukemia and mesotheliomas. In order to assess potential p27Kip1 gene alterations, we have screened a total of 147 human primary solid tumors and found no detectable cancer-specific mutations. These results argue that the often observed loss of antimitogenic transforming growth factor β responsiveness in human cancer cells is not due ...
Rat hepatoma-human fibroblast hybrids of two independent lineages containing only 8-11 human chromosomes show pleiotropic extinction of thirteen out of fifteen hepatic functions examined. Reexpression of the entire group of functions most often occurs in a block, and except for one discordant subclone, correlates with loss of human chromosome 2. The extinguished cells and their reexpressing derivatives have been examined for the expression of seven liver-enriched transcription factors. C/EBP, LAP, DBP, HNF3, and vHNF1 expression are not systematically extinguished in parallel with the hepatic functions. However, HNF1 and HNF4 show a perfect correlation with phenotype: these factors are expressed only in the cells showing pleiotropic reexpression. Since recent evidence indicates that HNF4 controls HNF1 expression, it can be proposed that the HNF4 gene is the primary target of the pleiotropic extinguisher. ...
Two groups have uncovered new wrinkles in reactions involving heterotrimeric guanosine triphosphate (GTP)-binding proteins (G proteins). Ligand binding to G protein-coupled receptors (GPCRs) activates G proteins by catalyzing guanine nucleotide exchange and dissociation of the GTP-bound α subunit (Gα) from the βγ heterodimer (Gβγ). Both subunits are then free to activate various effectors. Ribas et al. partially purified a ligand-independent G-protein activator, NG108-15 G-protein activator (NG-GPA), from the neuroblastoma-glioma hybrid NG108-15. NG-GPA stimulated guanosine 5-O-thiotriphosphate (GTPγS) binding to purified brain G protein, as well as to recombinant Gαo and Gαi, and inhibited adenylyl cyclase activity in DDT1-MF-2 cell membranes. NG-GPAs stimulation of GTPγS binding, unlike GPCR-stimulated GTPγS binding, was unaffected by treatment with pertussis toxin, which prevents agonist-induced receptor interactions with the Gi family of G proteins, suggesting that NG-GPA ...
Plot: When Pokemon population shrank to dangerous numbers, a few scientists thought out a plan. Using the DNA of the few remaining Pokemon,they created Pokemon/human hybrids. Of course this process wasnt exactly legal, and the scientists had to do their experiments in a secret facility. They used to get willing participants but now they steal young children, teenagers, and Pokemon from their parents and trainers in the dead of night, using them to experiment on. Due to their being a black
Scullys experiments involved genetic manipulations. She was part of the hybridization experiments through gene therapy; as many others, she was a failure and but a step towards the successful alien/human hybrid. Byers and the Thinker find never seen before proteins in her blood, the result of "branched DNA" in her own DNA (proteins are synthesized following DNA information). This brached DNA was inserted into Scullys using "technology fifty years down the line […] The cutting edge of genetic engineering." (Langly). It is alien or hybrid DNA that was made inactive at the end of the tests, now its only waste product, "a biological poison". At some point it was active though, as proteins based on it were synthesized; Scully had been turned temporarily into some kind of hybrid. "Could be a tracking system." "Developmental stages of a biological marker." "Or something as insidious as grafting a human into something… inhuman." Among all the Lone Gunmens theories, the latter supporting advaned ...
Since the DNA has been proven to be from both a male human and female human (parents DUH!) the only way any other DNA could be introduced is by some kind of gene splicing or for both the parents to be part human and alien. This lack of 100% DNA proof (due to degredation) is what the OP is clinging to but it leads down a very mad wild path since we now have 3 alien human hybrids, the child and the parents! What about the grandparents .....and so it goes on ...
For those who dont remember, the "Visible Man" and "Visible Woman" were educational plastic model kits put out by a company called "Renwal" of Mineola, New York in the 1960s and early 70s. They were 1/5th scale representations of the human body, showing skeletal structure, organs and representations of the nervous system, and they looked like the creepy models you always stared at in your doctors office as a kid (either that or you stared at those weird calendars portraying industrious little bug/human hybrid communities involved in a variety of smurf-like activities....anyone remember what those things were called??). The Visible Man and Woman were really detailed kits, allowing the builder to assemble a human torso from bones on up. The body was clear plastic, and there was a large chest cavity allowing the builder to poke around and explore the arrangement of organs, or pretend to build a Frankenstein monster, whatever floated your boat ...
Americans still dont understand that there is an intense war going on between humans evil alien human hybrids so called royalists in every state within
Cuticchia, A., & Porter, C. J. (1996). The role of the Genome Data Base at the chromosome 1 workshop. Cytogenetics and Cell Genetics, 72(2-3), 147-147 ...
You can think about a cells genetics as a huge social network. We can then take the DNA sequences of the transcription factor footprints associated with each gene and predict the proteins bound to these regulatory regions, and in this way reconstruct the genetic regulatory networks in every cell type.
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DI-fusion, le Dépôt institutionnel numérique de lULB, est loutil de référencementde la production scientifique de lULB.Linterface de recherche DI-fusion permet de consulter les publications des chercheurs de lULB et les thèses qui y ont été défendues.
A hybrid vehicle configured to allow charging from a source external to the vehicle includes a connector electrically connecting a battery with an external commercial power supply, an absorption unit
Hybrid sales have plummeted alongside gas prices and the economy, and dealers are giving sweet deals to move hybrids off their lots. Are some current owners regretting buying their hybrids when the market was at its peak?
Hyundai IONIQ hybrid, a stylish family car with sporty and dynamic exterior lines. Learn more about its various and convenient functions and high performance with hybrid technology
Orbital Technologies announced on 8 Dec 2009 that it successfully static tested its big vortex hybrid rocket motor. Whats a vortex hybrid? Its a hybrid that controls the fuel regression rate and combustion stability by swirling or recirculating the oxygen that is injected into the cavity of the fuel grain ...
[103 Pages Report] Check for Discount on United States Medical Hybrid Imaging System Market Report 2017 report by QYResearch Group. In this report, the United States Medical Hybrid Imaging System...
Page contains details about Au-ZnO hybrid nanopyramids . It has composition images, properties, Characterization methods, synthesis, applications and reference articles : nano.nature.com
The hybrid cloud is evolving along a dichotomy as the year comes to a close: It is getting easier to deploy but more challenging to optimize.
Ford Escape Hybrid - Stop Safely Now - My problem has been resolved... and you wont believe what it was. After months of working on my vehicle and several helpful posts and advice from this board... I finally figured it out. The only
This video uses a business scenario to illustrate a hybrid blend of purchased, leased, and employee-owned devices. The role of the network administrator is emphasized.
vivomove® 3 is a stylish hybrid smartwatch with fitness and health monitoring features, smart notifications, and a connected GPS for tracking your daily activities.
Originally Posted by jberry Do you find this is better than running 122 on 153 than 144 (or even 136?!) Havent tested the v3.5 yet on 144 or 136 but I
Moreover, within the body fusion of myoblasts and formation of multinucleate fibers may be exemplified. They can also be allowed to fuse in vitro and form heterokaryons. Macrophages fuse around the foreign body or bacterial cells in the tissues. Bone cells are also known to undergo somatic cell fusion. Cells growing in culture are induced by some of the viruses such as Sendai virus to fuse and form hybrids. This virus induces two different cells first to form heterokaryon (Fig. 6.7). During mitosis chromosome of heterokaryons are brought towards two poles which later on fuse to form hybrids. Removal of surface carbohydrates is necessary before establishment of cell fusion. Some chemicals such as polyethylene glycol also induce somatic cell fusion. It is interesting to note that the cells of taxonomically different animals can fuse and form hybrids. This suggests that there is no compatibility between membranes, nuclei, organelles of two different groups of animal cells (Sidebottom and ...
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Wait, wait, arthropod-like organisms with human CNS? A mammalian brain and spinal cord arent going to come close to functioning with arthropod physiology. For one thing, the human brain and spinal cord require a closed circulatory system for blood supply. A brain with that many condensed folds will not receive sufficient oxygen just by floating in a bath of hemolymph, plus the spinal cord needs a notochord and vertebral column for support or its delicate fibers will collapse under their own weight. The brain itself, one could conceivably shove in an arthropod cephalon of sufficient size for mere physical support, but it would still die from lack of oxygen, fail to connect with any of the radically different sensory/motor nerves of the arthropod head, crowd out the mouthparts, and otherwise fail for a dozen reasons. Secondly, where will said mammalian CNS originate from, and how, given that arthropods are protostomes and we are deuterostomes? You do know that even in the very beginning of zygote ...
Microcell-mediated chromosome transfer (MMCT) is a technique by which single or small numbers of chromosomes can be transferred from one mammalian cell to another by microcell fusion [1-3]. This technique can move the large intact genomic structures of natural chromosomes or artificially engineered chromosomes, and transferred chromosomes can be stably retained and freely segregate in recipient cells. Taking advantage of these features, MMCT has been employed very successfully in various basic science studies, e.g., genetic mapping and identification of tumor suppressor genes, analysis of genomic imprinting and production of animal models of disease [4-7]. Furthermore, MMCT is also used in gene transfer using a human artificial chromosome (HAC), mini-chromosome vector. HACs have several unique characteristics as gene-delivery vectors, including stable episomal maintenance in mammalian cells, the capacity to carry large transgenes, and less susceptibility to gene silencing, and have been applied ...
The work which I have been asked to review began a little over a year ago with an experiment which I made in collaboration with Dr J. F. Watkins (Harris & Watkins 1965). We showed that an animal virus, killed by irradiation with ultraviolet light, could be used to fuse together cells derived from mouse and man to produce artificial man-mouse hybrid cells. The idea of using viruses in this way has its origins in observations which go back for more than a century. (For a review of the relevant literature see Harris, Watkins, Ford & Schoefl 1966). Many diseases have long been known to be associated with lesions in which multi-nucleate cells are found. In the medical literature of the nineteenth century there is a protracted and vigorous controversy about the mode of formation of these cells. Multinucleate cells are commonly found in the lesions produced by certain pathogenic viruses and, during the last decade, it has become clear that in at least some cases the virus produces the multinucleate ...
After taking part in a rather unusual fuel-economy test for its non-hybrid 2013 Fusion EcoBoost, Ford has announced its 2013 Fusion Hybrid has just been awarded its official gas mileage ratings by the EPA. Just like the 2013 C-Max Hybrid, the 2013 Fusion Hybrid has been rated at 47 mpg highway, 47 mpg city, and 47 mpg combined. That’s hardly...
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They are increasing significantly," said Todd Leutheuser, executive director of the Southland Motor Car Dealers Association. "The incentives are strong enough that Chevy Volts are moving extraordinarily well and the Toyota Prius is off the charts - it could almost be its own brand.". Theres no denying the efficiency of hybrids. A recent issue of US News & World Report ranked the 2013 Ford Fusion hybrid No. 1 out of a list of affordable, mid-sized hybrid vehicles.. With an average price ranging from $26,851 to $31,526, the Ford Fusion hybrid was touted for its "excellent fuel economy, strong performance and great reliability." The car is powered by a four-cylinder engine and electric motor that together produce 188 horsepower.. At 47 miles per gallon in both the city and highway, the 2013 Fusion Hybrid has one of the best fuel ratings in its class.. The Fusion was followed in the rankings by the 2013 Hyundai Sonata hybrid, the 2013 Toyota Camry hybrid and the 2013 Toyota Prius V hybrid.. Others ...
In this article it is demonstrated that DSBs can induce homologous recombination between ectopic sequences in a higher eukaryote. In a recent study it was reported that the presence of the transposable element Ac can induce ectopic homologous recombination in somatic plant cells (Shalev and Levy 1997). The authors postulated that this was due to transiently induced breaks during transposition. The data presented here now fully sustain this hypothesis. Recently, however, it was also reported that Ac does not induce meiotic recombination (Dooner and Martinez-Ferez 1997). Thus the effect might be specific for somatic tissue.. It is becoming more and more apparent, especially for plants, that there are major differences between homologous recombination in somatic and meiotic cells. Our previous studies on the repair of DSBs in somatic plant cells by means of homologous sequences supplied by T-DNA molecules led us to the conclusion that the classical DSB repair model suggested for meiotic ...