EPC-CM promotes HUVEC proliferation and migration.EPC-CM incubation significantly increased proliferation (A) and migration (B) of HUVEC in comparison to contro

TY - JOUR. T1 - Sphingosine 1-Phosphate Protects Human Umbilical Vein Endothelial Cells from Serum-deprived Apoptosis by Nitric Oxide Production. AU - Kwon, Young Guen. AU - Min, Jeong Ki. AU - Kim, Ki Mo. AU - Lee, Doo Jae. AU - Billiar, Timothy R.. AU - Kim, Young Myeong. PY - 2001/4/6. Y1 - 2001/4/6. N2 - Sphingosine 1-phosphate (S1P) can prevent endothelial cell apoptosis. We investigated the molecular mechanisms and signaling pathways by which S1P protects endothelial cells from serum deprivation-induced apoptosis. We show here that human umbilical vein endothelial cells (HUVECs) undergo apoptosis associated with increased DEVDase activity, caspase-3 activation, cytochrome c release, and DNA fragmentation after 24 h of serum deprivation. These apoptotic markers were suppressed by the addition of S1P, the NO donor S-nitroso-N-acetylpenicillamine (100 μM), or caspase-3 inhibitor z-VAD-fmk. The protective effects of S1P were reversed by the nitric-oxide synthase (NOS) inhibitor ...

Human umbilical vein endothelial cells (HUVEC) are commonly used as a laboratory model system for the physiological and pharmacological investigations. This video describes how to derive HUVEC from the endothelium of veins of the umbilical cord. - Derivation of Human Umbilical Vein Endothelial Cells (HUVEC) - AbVideo™ - Support - Abnova

The involvement of ecto-5′-nucleotidase (E-5′Nu) in the elevation of extracellular adenosine during inflammation is unclear. In the present study, the effect of lipopolysaccharide (LPS), an inflammation inducer, was investigated on E-5′Nu in human umbilical vein endothelial cells (HUVECs). E-5′Nu activity was enhanced after a 24 h exposure to LPS. This effect was dose dependent, with an EC 50 of 1.66 ng/ml. At 10 μM, the phosphatidylinositol 3-kinase (PI3K) inhibitor LY-294002 abolished the LPS-induced E-5′Nu activity. However, at 10 μM, the NF-κB inhibitor ammonium pyrrolidine dithiocarbamate had no effect. LPS upregulated the protein expression but not the messenger RNA expression of E-5′Nu. The inhibition of E-5′Nu by 100 μM α,β-methylene adenosine-5′-diphosphate increased the LPS-induced inflammation, suggesting that E-5′Nu plays a significant role in reducing inflammation, probably through the generation of adenosine. In conclusion, the experiments indicate that LPS ...

https://doi.org/10.18632/oncotarget.24110 Nana Ai, Cheong-Meng Chong, Weiting Chen, Zhe Hu, Huanxing Su, Guokai Chen, Queenie Wing Lei Wong, Wei Ge

https://doi.org/10.18632/oncotarget.17298 Li-Chun Zheng, Xiao-Qing Wang, Kun Lu, Xiao-Ling Deng, Cheng-Wei Zhang, Hong Luo, Xu-Dong Xu, Xiao-Man Chen, Lu Yan, Yi-Qing Wang, Song-Lin Shi

Sigma-Aldrich offers abstracts and full-text articles by [Xiaoqin Mu, Kaiwen He, Hui Sun, Xin Zhou, Lingling Chang, Xin Li, Wenfeng Chu, Guofen Qiao, Yanjie Lu].

MATERIAL AND METHODS: Human umbilical vein endothelial cells (HUVECs) were treated with 1 µg/ml LPS in the absence or presence of 10 µM rosiglitazone for 24 h. Cell viability was measured by MTT assay. Flow cytometry was used to examine the cell apoptosis and ROS production in HUVECs response to LPS and rosiglitazone. The levels of pro-inflammatory cytokine factors, including TNF-α, IL-6, CXCL12, and CXCR4, were measured by ELISA, real-time PCR, and Western blot assay, respectively. The expression of PPARg, Bcl-2, and Bax and the activity of JAK2 and STAT3 were also investigated by Western blot assay ...

confirmed for the first time that HepG2-derived VASN can be transferred to human umbilical vein endothelial cells (HUVECs) via receptor mediated endocytosis of exosomes, at least in part through ...

Video articles in JoVE about human umbilical vein endothelial cells include High-resolution Time-lapse Imaging and Automated Analysis of Microtubule Dynamics in Living Human Umbilical Vein Endothelial Cells, Study of the Actin Cytoskeleton in Live Endothelial Cells Expressing GFP-Actin, Preparation and In Vitro Characterization of Magnetized miR-modified Endothelial Cells, Microfluidic Assay for the Assessment of Leukocyte Adhesion to Human Induced Pluripotent Stem Cell-derived Endothelial Cells (hiPSC-ECs), Gene Expression Analysis of Endothelial Cells Exposed to Shear Stress Using Multiple Parallel-plate Flow Chambers, Incorporating Pericytes into an Endothelial Cell Bead Sprouting Assay, An In Vitro 3D Model and Computational Pipeline to Quantify the Vasculogenic Potential of iPSC-Derived Endothelial Progenitors, Porphyromonas gingivalis as a Model Organism for Assessing Interaction of Anaerobic Bacteria with Host Cells, A Multi-well Format Polyacrylamide-based Assay for Studying

Human umbilical vein endothelial cells (HUVECs, Lonza) were cultured in EGM-2 (Lonza) and only used between passage 2C4. a novel heterotypic cell contact mediated signaling role for RhoA, as well as yield mechanistic insight into the ability of cells within the tumor microenvironment to facilitate steps of the metastatic cascade. cell-cell contacts (58), it remained unclear how cell contacts regulate RhoA activity in real-time. Additionally, although the EGF/CSF-1 paracrine loop of signaling was identified between tumor cells and macrophages, the intracellular signaling pathways induced by macrophages in the tumor microenvironment were elusive. Indeed, the EGF/CSF-1 paracrine loop of signaling is also required for both macrophage-induced invadopodium formation and transendothelial migration (Figure S9). However, as these are known to be secreted molecules, it remains to be determined which upstream contact-mediated signaling between cells in the tumor microenvironment is important for ...

TY - JOUR. T1 - Imaging of angiogenesis of human umbilical vein endothelial cells by uptake of exosomes secreted from hepatocellular carcinoma cells. AU - Yukawa, Hiroshi. AU - Suzuki, Kaoru. AU - Aoki, Keita. AU - Arimoto, Tomoko. AU - Yasui, Takao. AU - Kaji, Noritada. AU - Ishikawa, Tetsuya. AU - Ochiya, Takahiro. AU - Baba, Yoshinobu. N1 - Funding Information: This research was mainly supported by the Development of Diagnostic Technology for Detection of miRNA in Body Fluids grant from the Japan Agency for Medical Research and Development and New Energy and Industrial Technology Development Organization and by the Nanotechnology Platform Program (Molecule and Material Synthesis) of the Ministry of Education, Culture, Sports, Science and Technology (MEXT). This work was partially supported by a Research Center Network for Realization of Regenerative Medicine grant from the Japan Agency for Medical Research and Development and JSPS KAKENHI Grant Numbers JP26790006 and 17H02731. We ...

Xie, W., Xie, H., Liu, F., Li, W., Dan, J., Mei, Y., Dan, L., Xiao, X., Li, J. and Chen, X. (2013), Propranolol induces apoptosis of human umbilical vein endothelial cells through downregulation of CD147. British Journal of Dermatology, 168: 739-748. doi: 10.1111/bjd.12193 ...

The purpose of the present study was to investigate the effect of microRNA (miR)‑144‑5p on human umbilical vein endothelial cells (HUVECs) to explore the role of miR‑144‑5p in atherosclerosis. miR‑144‑5p expression was upregulated in HUVECs using miR‑144‑5p mimics. The relative expression level of miR‑144‑5p in HUVECs was detected using reverse transcription‑quantitative PCR (RT‑qPCR). Cell proliferation was detected by performing an MTT assay. Apoptosis was determined via flow cytometry. Cell migration ability was detected by a wound‑healing assay. Cell invasion was determined by a transwell assay. The protein levels of phosphorylated (p)‑PI3K, p‑Akt and endothelial nitric oxide synthase (eNOS) were detected using western blot analysis. The binding sites between miR‑144‑5p and 3‑untranslated region of rapamycin‑insensitive companion of mTOR (RICTOR) mRNA were predicted by TargetScan and confirmed by a dual luciferase reporter assay. The present study ...

Human umbilical vein endothelial cells stained initially for nuclei with DAPI (blue) and for vascular endothelial cadherin (red).

The fuel sensing enzyme AMP-activated protein kinase (AMPK) enhances processes that generate ATP when stresses such as exercise or glucose deprivation make cells energy deficient. We report here a novel role of AMPK, to prevent the activation of NF-kappaB in endothelial cells exposed to the fatty ac …

AKT (a serine/threonine protein kinase) has become a popular target for drug discovery campaigns, due to the fact that AKT inhibitors may help to treat a number of cancers. In this application note BioTek demonstrates an automated homogeneous assay to probe AKT phosphorylation at its serine 473 residue using endogenous levels of kinase expression within human primary HUVEC cells.

In the present study we try to investigate the effect of Sodium Ferulate (SF) on Oxidized Low-Density Lipoprotein (Ox-LDL) induced IL-8 expression in ..

2-methoxyoestradiol (2-MeOE2) is a potent anti-angiogenic agent. Its 3- and 17-sulphamoylated derivatives have been demonstrated to induce G2-M cell cycle arrest and apoptosis in breast cancer cells in vitro as well as tumour regression in rats in vivo with greater potency than the parent oestrogen. To determine whether the anti-cancer properties of these derivatives can be synergistically enhanced with low-dose TNF-alpha co-treatment, we investigated the effects of these treatments in adult human fibroblasts and human umbilical vein endothelial cells (HUVECs). Treatment of fibroblasts with 0.1 microM 2-methoxyoestradiol-3,17-bis sulphamate (2-MeOE2bisMATE) but not 2-MeOE2 caused a reversible morphology change and induced G2-M arrest (from 12 to 33%) but not subsequent apoptosis. In contrast, treatment of HUVECs did not induce morphology change or G2-M arrest. Using a nucleosomal ELISA assay, we showed that TNF-alpha (20 ng/ml) combination treatment synergistically increases 0.1 microM 2-MeOE2bisMATE

4785 Natural Killer Transcript 4 (NK4) is a novel human gene that was first identified in IL-2 stimulated natural killer cells or mitogens stimulated T cells. Besides lymphocytes, our microarray data showed an induction of NK4 in human umbilical vein endothelial cells (HUVEC) when Akt was activated. Since Akt is important in cell survival signaling pathway, and NK4 was originally found in cytokines stimulated lymphocytes, we hypothesized that NK4 may play an important role in the inflammation angiogenesis. Proteins involved in angiogenesis such as VEGF, Ang1, or Ang2 did not influence NK4 transcription. The major inflammatory cytokines, IL-1 or TNFα, were found to induce NK4 expression. Those cytokines could induce NK4 mRNA when Akt activity was abolished by adenovirus dominant negative Akt, but not when NFkB activation was disrupted by adenovirus IKB. Taken together, Akt activation was sufficient to induce NK4 transcription, however that was not required for IL-1- or TNFα-meidated induction ...

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene, which is upregulated in human umbilical vein endothelial cells, encodes a G protein-coupled receptor. Variations in this gene can affect a person's stature. Multiple transcript variants encoding different proteins have been found for this gene. [provided by RefSeq, Mar 2009 ...

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TY - JOUR. T1 - Comparative profiling of stemness markers expressed by human umbilical vein endothelial cells and human umbilical cord matrix-derived stem cells. AU - Hayati, Abdul Rahman. AU - Adzman-Sellymiah, AU - Kien Hui, Chua. AU - Aqilah, Raman Nur Fatin. AU - Tan, Geok Chin. AU - Fatimah, Simat Siti. AU - Lim, Yun Hsuen. AU - Syahrina, Rahim Nur. AU - Fariha, Mohd Manzor Nur. PY - 2018/12/28. Y1 - 2018/12/28. N2 - Mesenchymal stem cells population has been successfully isolated from the matrix and characterised for its stemness properties. Endothelial cells from the vein of the umbilical cord which are usually termed as HUVEC are also of interest to researchers as it can be manipulated in vitro to study endothelial cells behaviour and vascular-associated diseases. Immunophenotyping for positive and negative markers of mesenchymal stem cells was performed on HUVEC and compared to the closest stem cells that were isolated from the umbilical cord matrix which is termed as HUCMSC in this ...

Abdominal aortic aneurysm (AAA) is a great threat to the health of elder (,50 years old) individuals. High salt intake is considered to raise the risk of AAA but the underlying mechanism remains to be elucidated. As endothelial dysfunction in the abdominal aorta is strongly associated with AAA, the present study hypothesized that high salt led to AAA by inducing apoptosis of endothelial cells. The present study verified that hypertonic medium with excess sodium chloride induced apoptosis of human umbilical vein endothelial cells (HUVECs), a commonly used cell model to study aortic endothelial cells. Further mechanism studies suggested that hypertonic conditions elevated the expression of nuclear factor of activated T cells 5 (NFAT5) and a high level of NFAT5 was capable of inducing apoptosis of HUVECs. In the investigation of downstream signals of NFAT5, it was identified that either hypertonic conditions or NFAT5 overexpression promoted the activity of NF‑κB signaling pathway and ...

Thejass, P.; Kuttan, G., 2007: Inhibition of angiogenic differentiation of human umbilical vein endothelial cells by diallyl disulfide (DADS)

TY - JOUR. T1 - The polyphenol delphinidin induces antioxidant effects in human umbilical vein endothelial cells through activation of endogenous glutathione: importance of using relevant concentration in in vitro systems. AU - Goszcz, Katarzyna. AU - Duthie, Garry. AU - Stewart, Derek. AU - Megson, Ian. N1 - © 2017, Published by the BMJ Publishing Group Limited.. PY - 2017/4/1. Y1 - 2017/4/1. N2 - Polyphenols are regarded to have a wide range of health-promoting effects. Increased consumption of polyphenol-rich food is known to be associated with numerous cardioprotective effects. Polyphenols have been shown to improve endothelial function, inhibit abnormal platelet aggregation, reduce inflammation and improve plasma lipid profiles. Moreover, polyphenols have been widely recognised as powerful antioxidants. Given that oxidative stress plays a key role in initiation and progression of atherosclerosis, antioxidant therapy with polyphenols has potential. However, the concentrations required to ...

We studied the effects of a C60 water suspension at 4 microg/mL (nC60) and the water soluble fullerenol C60(OH)24 at final concentrations of 1-100 microg/mL on human umbilical vein endothelial cells (HUVECs) in culture. We found that a 24 hr treatment of HUVECs with C60(OH)24 at 100 microg/mL signif …

Pathways in biological system often cooperate with each other to function. Changes of interactions among pathways tightly associate with alterations in the properties and functions of the cell and hence alterations in the phenotype. So, the pathway interactions and especially their changes over time corresponding to specific phenotype are critical to understanding cell functions and phenotypic plasticity. With prior-defined pathways and incorporated protein-protein interaction (PPI) data, we counted PPIs between corresponding gene sets of each pair of distinct pathways to construct a comprehensive pathway network. Then we proposed a novel concept, characteristic sub pathway network (CSPN), to realize the phenotype-specific pathway interactions. By adding gene expression data regarding a given phenotype, angiogenesis, active PPIs corresponding to stimulation of interleukin-1 (IL-1) and tumor necrosis factor α (TNF-α) on human umbilical vein endothelial cells (HUVECs) respectively were derived. Two

The tumor growth and metastasis of several cancers depend on the extent of both angiogenesis and lymphangiogenesis triggered by chemical signals that are originating from cancer cells with aggressive growth ability. The discovery of angiogenic inhibitors have been helping to reduce both morbidity and mortality resulting from multiple types of cancers. As part of the comprehensive treatment strategy combining anti-angiogenic agents with conventional cytoreductive treatments is considered to be the most effective approach. As part of this strategy an added ability for inducing apoptosis in cancer cells could make the therapeutic agents very effective. The in vitro anti-angiogenic activity of water soluble form of JFD (JFD-WS) was examined by ECMatrixTM gel assay with human umbilical vein endothelial cells (HUVEC) using 0.01-10 μM concentrations at different time intervals (0, 4 and 8 hrs). A complete inhibition of in vitro angiogenesis was observed in 8 hrs at 10 μM concentration of JFD-WS. Our ...

Our lab previously characterized the use of a multi-channel microfluidic glomerular device containing both biological (Human Umbilical Vein Endothelial Cells (HUVECs)) and artificial physical (8nm polyethersulferone (PES) membranes) mechanisms of filtration. This model was found to filter 71% BSA-FITC in solution. However, in-vivo, cells are responsible for filtration through vein endothelial cells and podocytes, cells fundamental to filtration due to slit diaphragms, within the glomerulus.. Our current focus is on improving this glomerular device by the addition of conditionally immortalized human podocyte cells (CIHP-1) and use of larger pore size membranes incapable of filtering to create a more realistic model. Before doing so, membranes were exposed to flow and found to maintain shape, cells were identified by light microscopy and immunohistochemistry, and coatings were optimized to keep cells on membranes during flow. Podocytes slit diaphragms allow them to filter small to large molecules ...

Definition of umbilical vein, with etymology, pronunciation (phonetic and audio), synonyms, antonyms, derived terms and more about the word umbilical vein.

Cardiovascular disease is associated with androgen hormones, particularly testosterone. Platelets play an important role in cardiovascular events as it can undergo inappropriate activation, adhesion, and aggregation, thus supporting the formation of a thrombus. The attachment of platelets to endothelial cells is influenced by CD40-CD40L interaction. Hyperglycemia can increase platelet reactivity and activate endothelial cells. To examine the influence of testosterone to the expression of CD40 in hyperglycemic endothelial cells, we did an in vitro laboratory study using post test only control group design. Study subject was the primary culture of endothelial cells derived from human umbilical vein endothelial cells (HUVEC). Independent variables were testosterone that was given in 3 doses: 1 nM, 10 nM, and 100 nM. High glucose environment was given in 22,4 mM. The expression of endothelial CD40 was measured using enzyme-linked immunosorbent assay (ELISA). This study showed that endothelial CD40 ...

The aim of this study was to analyse the potential roles of protein kinase enzymes in tumour necrosis factor-alpha (TNF-alpha) and interleukin-1 (IL-1) induced expression of the adhesion molecules intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) on human umbilical vein endothelial cells (HUVEC). The authors observed a marked increase in ICAM-1 and VCAM-1 expression on HUVEC stimulated for 24 h by TNF-alpha (10 ng/ml) or IL-1 (20 ng/ml). Pre-treatment of HUVEC for 30 min with protein tyrosine kinase (PTK) inhibitors genistein and herbimycin A (10 micrograms/ml and 0.5 microgram/ml, respectively) before stimulation with IL-1 did not affect the expression of these molecules. Similar results were observed with respect to VCAM-1 expression on HUVEC stimulated by TNF-alpha. In contrast, pre-incubation of HUVEC with PTK inhibitors prior to the addition of TNF-alpha significantly enhanced subsequent expression of ICAM-1, although spontaneous expression of ICAM-1 on

MicroRNAs (miRNAs) are small non-protein-coding RNAs that are incorporated into the RNA-induced silencing complex (RISC) and inhibit gene expression by regulating the stability and/or the translational efficiency of target mRNAs. p75NTR, which is scarcely present in healthy endothelial cells (ECs), becomes strongly expressed by capillary ECs after induction of peripheral ischemia in type-1 diabetic mice. p75NTR expression promotes endothelial cells apoptosis and inhibits angiogenesis. In order to identify miRNAs sub-sequentely modulated by p75NTR, miRNA expression profiles of human umbilical vein endothelial cells (HUVEC) over-expressing p75NTR were generated, allowing the identification of miRNAs modulated upon p75NTR up-regulation.

Plantago asiatica has been traditionally used for traditional medicine around East Asia. Plantamajoside (PM), which is isolated from this plant, is known for biological properties including anti-inflammation and antioxidant activity. To demonstrate the biological activity of PM against endothelial dysfunction induced by advanced glycation end-products (AGEs), a cellular inflammatory mechanism system was evaluated in human umbilical vein endothelial cells (HUVECs). We obtained PM through previous research in our laboratory. We formed the AGEs from bovine serum albumin with glyceraldehyde in the dark for seven days. To confirm the modulation of the inflammatory mechanism in endothelial dysfunction, we quantified the various pro-inflammatory cytokines and endothelial dysfunction-related proteins in the HUVECs with Western blotting and with real-time and quantitative real-time polymerase chain reactions. Co-treatment with PM and AGEs significantly suppressed inflammatory

Endothelial Cell Growth Kit-BBE (ATCC ® PCS-100-040) and Endothelial Cell Growth Kit-VEGF (ATCC ® PCS-100-041) each contain components that when added to Vascular Cell Basal Medium (ATCC ® PCS-100-030) create a complete ATCC ® Primary Cell Solution™ culture environment for endothelial cells derived from normal human large vessels (e.g., Normal Primary Human Umbilical Vein Endothelial Cells (HUVEC), ATCC ® PCS-100-010 or Primary Aortic Endothelial Cells, ATCC ® PCS-100-011). Your experimental design will dictate which Endothelial Cell Growth Kit should be used. Use of the Endothelial Cell Growth Kit-VEGF (ATCC ® PCS-100-041) will support a faster rate of proliferation because of the presence of several purified human recombinant (rh) growth factors (rh VEGF, rh EGF, rh FGF basic and rh IGF-1) combined with heparin and hydrocortisone. Use of the Endothelial Cell Growth Kit-BBE (ATCC ® PCS-100-040), which contains Bovine Brain Extract (BBE), is recommended if a less

Endothelial Cell Growth Kit-BBE (ATCC ® PCS-100-040) and Endothelial Cell Growth Kit-VEGF (ATCC ® PCS-100-041) each contain components that when added to Vascular Cell Basal Medium (ATCC ® PCS-100-030) create a complete ATCC ® Primary Cell Solution™ culture environment for endothelial cells derived from normal human large vessels (e.g., Normal Primary Human Umbilical Vein Endothelial Cells (HUVEC), ATCC ® PCS-100-010 or Primary Aortic Endothelial Cells, ATCC ® PCS-100-011). Your experimental design will dictate which Endothelial Cell Growth Kit should be used. Use of the Endothelial Cell Growth Kit-VEGF (ATCC ® PCS-100-041) will support a faster rate of proliferation because of the presence of several purified human recombinant (rh) growth factors (rh VEGF, rh EGF, rh FGF basic and rh IGF-1) combined with heparin and hydrocortisone. Use of the Endothelial Cell Growth Kit-BBE (ATCC ® PCS-100-040), which contains Bovine Brain Extract (BBE), is recommended if a less

Abcell-bio offers mature endothelial cells isolated from human umbilical cord vein.. These adherent cells express all the specific markers of endothelial cells: CD31, CD144 and KDR. Each HUVEC batch is derived from a single, healthy donor. Fresh HUVEC produced by Abcell-bio are provided in EndoMac2.2 medium, at passage 1,2,or 3. Cells are provided in a 25-cm2 flask.. ...

Recent findings indicate that specific microRNAs (miRNAs), such as those of the miR-17-92 cluster, may be responsible for regulating endothelial gene expression during tumor angiogenesis. Secreted miRNAs enclosed in exosomes also have an important role in cell-cell communication. To elucidate whether miRNAs secreted from neoplastic cells transfer into endothelial cells and are functionally active in the recipient cells, we investigated the effect of exosomal miRNAs derived from leukemia cells (K562) on human umbilical vein endothelial cells (HUVECs). As K562 cells released the miR-17-92 cluster, especially miR-92a, into the extracellular environment, K562 cells, transfected with Cy3-labeled pre-miR-92a, were co-cultured with HUVECs. Cy3-miR-92a derived from K562 cells was detected in the cytoplasm of HUVECs, and the Cy3-miR-92a co-localized with the signals of an exosomal marker, CD63. The expression of integrin α5, a target gene for miR-92a, was significantly reduced in HUVECs by exosomal ...

Increased generation of reactive oxygen species (ROS) in hyperglycaemia is linked to endothelial cell DNA damage in diabetes. Nuclear and/or mitochondrial DNA (mtDNA) damage may accelerate ageing of endothelial cells and, in part, account for the endothelial dysfunction associated with the pathogenesis of many cardiovascular diseases. The aim of this thesis was to investigate cell ageing in human endothelial cells, by studying the role of ROS in telomere attrition after exposure to increased glucose levels. This thesis also analysed the effects of mtDNA depletion on the pro-inflammatory phenotype of endothelial cells. Human umbilical vein endothelial cells (HUVECs) were treated with high glucose (HG; 22mM) or alternating normal (5.5mM) /HG (AG; to mimic post-prandial fluctuations in glucose). Telomere attrition rate, measured globally across all chromosomes using Southern blotting and specifically on the XpYp chromosome using single telomere length analysis (STELA) were increased 3-6-fold in ...

VEGF is a key angiogenic cytokine and a major target in antiangiogenic therapeutic strategies. In endothelial cells (ECs), VEGF binds VEGF receptors and activates ERK1/2 through the phospholipase gamma (PLC gamma)-PKC alpha-B-Raf pathway. Our previous work suggested that influx of extracellular Ca(2+) is required for VEGF-induced ERK1/2 activation, and we hypothesized that this could occur through reverse mode (Ca(2+) in and Na(+) out) Na(+)-Ca(2+) exchange (NCX). However, the role of NCX activity in VEGF signaling and angiogenic functions of ECs had not previously been described. Here, using human umbilical vein ECs (HUVECs), we report that extracellular Ca(2+) is required for VEGF-induced ERK1/2 activation and that release of Ca(2+) from intracellular stores alone, in the absence of extracellular Ca(2+), is not sufficient to activate ERK1/2. Furthermore, inhibitors of reverse mode NCX suppressed the VEGF-induced activation of ERK1/2 in a time-and dose-dependent manner and attenuated ...

Extracts of raw and ripe Pu-erh tea were prepared from tea dust during processing.The antioxidtive activities of extracts were evaluated by ABTS and FRAP systems.The potentially protective effects of extracts on human umbilical vascular endothelial cells(HUVEC) were investigated in Na2S2O3-induction model.The results showed that the extract of raw Pu-erh tea had stronger antioxidtive activity than that of ripe Pu-erh tea in both test systems.But extract of ripe Pu-erh tea showed a less protective effect than that of extract of raw Pu-erh tea on the HUVEC cell damage induced by Na2S2O3,and relevent with tea polyphenols under certain concentrations.

Previously, we reported that a predominant action of a type-1 insulin-like growth factor receptor (IGF-1R)-targeted antibody was through inhibiting tumor-derived VEGF, and indirectly, angiogenesis. Here, we examined the direct antiangiogenic activity of the IGF-1R-targeted antibody SCH717454 that inhibits ligand-receptor binding and the mechanism by which tumors circumvent its antiangiogenic activity. Inhibition of ligand-stimulated activation of IGF-1R, insulin receptor (IN-R), or downstream signaling [phosphorylation of Akt (Ser473)] was determined by receptor-specific immunoprecipitation and immunoblotting. Inhibition of angiogenesis was determined by proliferation and tube formation using human umbilical vein endothelial cells (HUVEC) in vitro and in Matrigel plugs implanted in mice. SCH717454 blocked IGF-1-stimulated but not IGF-2-stimulated phosphorylation of Akt in sarcoma cells. Immunoprecipitation using anti-IGF-1R and anti-IN-R antibodies revealed that SCH717454 equally blocked ...

LIVas EnGS (containing Endothelial Cell Growth Supplement) is a new low serum medium optimized for the culture of human endothelial cells including Human Umbilical Vein Endothelial Cells (HUVEC), aortic endothelial cells and other human large-vessel endothelial cells. LIVas supports the growth of these cells in a low serum environment without human VEGF. Creative Bioarrays cell culture medium contains no antimicrobials and no phenol red since these components can cause cell stress and masking effects that may influence experimental results ...

Mouse monoclonal antibody raised against native human MCAM. Human umbilical vein endothelial cells (HUVECs). (MAB15401) - Products - Abnova

bromelain protease F9: selectively decreases the CD44-mediated binding of peripheral blood lymphocytes (PBL) to human umbilical vein endothelial cells (HUVEC)

Human Placental Vascular Endothelial Cell MicroRNA https://www.sciencepro.com.br/produtos/sc-7107 https://www.sciencepro.com.br/@@site-logo/logo-novo.png ...

Localization of tPA and vWf in endothelial cells: immunogold labeling in HUVEC of tPA (a and b) and of vWf (c), and immunogold labeling of tPA in murine ca

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專辦英國留遊學, 歐美紐澳留遊學, 顧問皆有十年以上的諮詢經驗, 為英美多所中學及大專院校的海外辦事處, 提供獎學金申請服務及資訊, VEC英國辦事處更提供學生後續支援及監護服務, 專業親切的客制化申請服務, 是專屬也是獨一無二的。