Intracellular bacterial pathogens have evolved a range of mechanisms, including manipulation of the host cell epigenetic machinery and host cell gene expression rewiring, to parasitize and thrive inside host phagocytes. A new study in The EMBO Journal (Yaseen et al, 2018) reports that, conversely, host macrophages can use epigenetic modulators to modify the cell surface of invading pathogens and counteract infection. This study opens new avenues to better understand host-pathogen interactions and to develop novel, more effective antimicrobial strategies.. See also: I Yaseen et al (January 2018) ...
Bacterial pathogens represent a major health problem worldwide. In developing countries they are one of the major killers (notably M. tuberculosis) particularly for immuno-compromised patients (aging people, HIV-infected patients, grafted patients) or people suffering from specific diseases (e.g. cystic fibrosis patients). One aggravating factor is the rise of new strains of bacteria resistant to one or several antibiotics, and the very limited number of newly developed antibiotics. This has led to a renewed interest in understanding how pathogenic bacteria can cause diseases.. Bacteria are defined as virulent (or pathogenic) based on their ability to mount a harmful infection in a host. Virulent bacteria can infect a host, non-virulent bacteria cannot. A successful infection relies on a series of specific bacterial traits, such as the ability to secrete toxins, to escape the host immune system, to replicate within host cells or to inhibit phagocytic engulfment. For any given pathogenic ...
Using DNA Microarrays to Study Host-Microbe Interactions, info about Medicine: Medical Specialties: Microbiology: Using DNA Microarrays to Study Host-Microbe Interactions
Deborah Hung Laboratory. We are facing an incredible challenge in the realm of infectious diseases that has been brought on by the convergence of three current phenomena - newly emerging infectious organisms, a global crisis in antibiotic resistance, and the threat of bioterrorism. A response to this challenge requires a renewed devotion to understanding the underlying mechanisms involved in infection and an increased commitment to the discovery of new antibiotics.. The goal of research in the Hung Lab is to understand in vivo mechanisms of bacterial pathogenesis by studying pathogen-host interactions. By merging the fields of chemical genetics and bacterial genetics/genomics, we hope to provide insight into possible new paradigms for addressing infectious diseases.. Despite recent, largely genetic, technical advances in the field of in vivo pathogen-host interactions, many important questions related to the mechanisms of bacterial pathogenesis remain unanswered, in part because of the inability ...
Our understanding of bacterial pathogenesis is dominated by the cell biology of the host-pathogen interaction. However, the majority of metabolites that are used in prokaryotic and eukaryotic physiology and signalling are chemically similar or identical. Therefore, the metabolic crosstalk between pathogens and host cells may be as important as the interactions between bacterial effector proteins and their host targets. In this Review we focus on host-pathogen interactions at the metabolic level: chemical signalling events that enable pathogens to sense anatomical location and the local physiology of the host; microbial metabolic pathways that are dedicated to circumvent host immune mechanisms; and a few metabolites as central points of competition between the host and bacterial pathogens.
Disease-causing microorganisms that find their way into feeds and foods or farms and homes can pose a threat to the health of the animal and human hosts. The Host - Pathogen Interface research cluster seeks to uncover both the common and unique ways that micro-organisms act to gain entry and produce disease in their hosts, and understand how protective barriers and defense mechanisms in animal tissues and cells work to combat bacterial, viral, and parasitic infectious agents. Through their discoveries, our investigators develop innovative approaches to prevent or ameliorate outbreaks of infectious diseases in humans, companion animals, and livestock. ...
We fund basic, clinical and translational research into pathogens implicated in human infectious diseases. This includes understanding of the pathogen at the molecular and cellular level through to host pathogen interactions and into epidemiology of infectious diseases. We are also keen to fund research to inform novel strategies for preventing and controlling infectious and immune disease control, including research on human behaviour and lifestyle.. ...
Single-molecule fluorescence spectroscopy and imaging, super-resolution microscopy. The extraordinary temporal and spatial precision of human infectious processes relies on the exact and specific interactions of host and pathogen. Research into these processes requires sophisticated analytical methods. The small size of most infectious agents (bacteria, viruses) and the subcellular compartments in the host requires imaging with a spatial resolution close to electron microscopy to obtain insights into the molecular structure and organization of host pathogen interactions. The project of Markus Sauer aims to develop refined localization microscopy methods (dSTORM and PALM) capable of providing quantitative information on the distributions and densities of molecules with unmatched spatial resolution. For example, the sub-cellular localization of bacterial proteins secreted by Chlamydia (Thomas Rudel), Salmonella (Jörg Vogel) and Campylobacter (Cynthia Sharma) and their interacting host proteins ...
In this study, comprehensive analyses of murine 65-kDa GBPs in host defense were performed. In addition to the five known mGBPs, five novel murine 65-kDa GTPases were identified by microarray and in silico analyses. Interestingly, all 10 mGBPs were up-regulated in vitro by IFN-γ, selected proinflammatory stimuli, and TLR agonists as well as in vivo upon infection with L. monocytogenes or T. gondii. Importantly, a set of mGBPs were found to enclose intracellular T. gondii parasites, defining them as key effector molecules at the interface of host pathogen interaction. Strikingly, virulent T. gondii interfered with the accumulation of mGBPs around the PV.. GBPs are characterized by their distinct GTP-binding motives G1-G4. The known 65-kDa mGBPs have canonical G1 and G3 GTP binding sites, but possess an RD in G4 instead of the N/T KXD motif found in other GTPases, such as Rab, Ras, and 47-kDa GTPases (12, 15). Mutational analysis of hGBP1 revealed that in the G4-binding motif of GBPs the amino ...
The editors of Pathogens and Disease aim to publish outstanding primary Research Articles, Short Communications, and MiniReviews reporting on hypothesis- or discovery-driven studies relating to pathogens, the host pathogen interaction, the host response to infection, and their molecular and cellular correlates.
[email protected] Research Focus: Host pathogen interaction, trafficking of AAV and VACV in multiple cell lines with emphasis on cytokines/ chemokines mediated inhibition of viruses, viral morphogenesis, signaling cascade and cell fate ...
Im an evolutionary biologist who studies bacteriophage life history stochasticity and the population dynamics of host/pathogen interactions Im currently affiliated with Queens College and the CUNY Graduate Center. I can be reached at john.dennehyATqc.cuny. ...
Im an evolutionary biologist who studies bacteriophage life history stochasticity and the population dynamics of host/pathogen interactions Im currently affiliated with Queens College and the CUNY Graduate Center. I can be reached at john.dennehyATqc.cuny. ...
The Jason Harris, MD, MPH and Regina LaRocque, MD, MPH Laboratory is focused on host-pathogen interactions and the innate and adaptive immune response to Vibrio cholerae, cholera vaccines, and in the area of susceptibility to cholera.
Tuberculosis kills between 2 million and 3 million people each year and continues to be a major global health concern. Mycobacterium tuberculosis, the etiologic agent responsible, is an obligate human pathogen that has infected mankind since the dawn of time. The emergence of highly drug-resistant forms of the disease threatens to completely undermine disease-control efforts and even may be shifting the fundamental pathobiology of the host-pathogen relationship. The community of scientists engaged in studying this deadly disease has made dramatic advances in understanding the biology and biochemistry of this deadly pathogen, but many important details are only now starting to be appreciated. These three symposia in the tuberculosis theme will bring together diverse speakers struggling to understand the molecular details of the host-pathogen relationship and how the bacterium may be adapting to human attempts to bring the disease under control. The subjects were chosen so that the three symposia ...
The Merck Research Laboratories (MRL) Exploratory Science Center (ESC) Cambridge, a wholly owned subsidiary of Merck and Co., is focused on driving early discovery research.
The Schultz group will add chemical expertise and tool development to the consortium. The tools provided will help understanding pathogen-host interactions as well as pathogen lifecycles. Specifically, we will prepare artificial amino acids and photostable membrane-permeant dyes for non-disruptive labeling of proteins in intact pathogens and host cells. We will apply new methods to cross-link interacting proteins inside cells. We will provide photoactivatable lipids to manipulate host membranes. Finally, we will provide fluorescent reporters for monitoring relevant enzyme activities. All tools will be applied in close collaboration with other groups from the consortium including Brügger, Müller, Ruggieri, Kräusslich, Lemke, Briggs, Fackler, Urban. Specifically, we will help to visualize rare molecules such as viral particles or dsRNA by helping other consortium members tagging them with dyes suitable for single molecule microscopy (Lemke, Kräusslich) or correlative electron microscopy ...
Dept. of Infectious Diseases - Virology. Malaria remains one of the most devastating infectious diseases in humans, for two reasons. One is that Plasmodium(the causative malaria agent) has evolved efficient means to exploit cellular host mechanisms in order to proliferate. The second are the persisting gaps in our understanding of these pathogen-host interactions, especially in the clinically silent liver stage. Our two labs have recently performed a collaborative project with the aim to dissect this particular step, using a physiologically relevant in vivo mouse model of Plasmodium infection. We specifically focused on the identification of mi(cro)RNAs (small regulatory RNAs that control gene expression in many species) that are dysregulated in livers of infected mice. Interestingly, we found that Plasmodium infection triggers a significant, up to ten-fold increase of a particular miRNAknown to be a key player in innate and adaptive immunity in mammals, miR-155 (Hammerschmidt-Kamper et al., ...
Multidisciplinary has become the watchword of modern biology. The integration of several disciplines has created a rapidly growing multidisciplinary field of research, the so-called Mathematical Immunology . In simple terms, mathematical immunology attempts to uncover the biological mechanisms underlying the dynamics of pathogen-host interactions by employing mathematical, statistical, and computational models. In recent years, the field of mathematical immunology has blossomed with the availability of large and rich datasets due to the genomics revolution and increased sensitivity of laboratory and clinical tools; the development of mathematical tools capable of encapsulating complex nonlinear systems; and the advancement in computing power for large-scale calculations, simulation, and visualization. Interest in the computer simulation of biological processes to reduce complications incurred in human and animal research (such as ethical and practical considerations, costs, and risks) has also ...
The Hultquist lab specializes in the development and adaptation of high-throughput, quantitative, systems-based approaches for use in primary models of disease to better understand the host-pathogen relationship. Leveraging diverse expertise in primary cell models, proteomic profiling, and functional genomics, we work at the intersection of systems biology and infectious disease, defining host-pathogen interactions and their consequences for replication and pathogenesis. Through these efforts, we ultimately hope to strengthen the bridge from big data to targeted discovery to clinical application for the development of personalized, host-driven therapies and the advancement of human health. ...
MICROBE and HOST LIPIDS. From infections to microbiota and imaging. Microbes are found in almost every habitat present in nature and are vital to humans. For instance, the human microbiota has an influence on host physiology. In contrast, some pathogenic microbes can cause infectious diseases, which altogether claim 17 million deaths per year worldwide. Better understanding man and microbes interaction is thus a major public health issue. Indeed, a deeper knowledge of microbiota functions as well as the pathogenic mechanisms developed by microbes will pave the way for the development of new preventive or therapeutic strategies.. Lipids, via their different functions, play a key role in host-microbe interactions. They are essential components of the cell membranes. Some of them are able to modulate host cell functions, and might be used as virulence factors by pathogenic microbes. Manipulation of host lipid metabolism is among the invading strategies evolved by infectious agents, but regulation ...
Why is a wholesome person protected from attacks while people with cystic fibrosis or damaged epithelium are particularly vunerable to this opportunistic pathogen? To be able to address this issue it is vital to completely understand the powerful interplay between your web host microenvironment and host-pathogen connections within a physiologically relevant way. pathogenesis can help progress fundamental knowledge of pathogenic systems and raise the translational potential of analysis results from bench towards the patients bedside. Launch As a respected reason behind life-threatening nosocomial attacks has been thoroughly studied within the last 35 years. Zero available or super model tiffany livingston program can mimic the condition phenotype completely. Accordingly the intricacy of the web host pathogen and host-pathogen connections provides necessitated a mixed usage of different model systems to progress our knowledge of infectious disease. is certainly most commonly BRL 52537 ...
in Journal of virology (2016), 90(4), 2039-51. Carbohydrates play major roles in host-virus interactions. It is therefore not surprising that, during coevolution with their hosts, viruses have developed sophisticated mechanisms to hijack for their ... [more ▼]. Carbohydrates play major roles in host-virus interactions. It is therefore not surprising that, during coevolution with their hosts, viruses have developed sophisticated mechanisms to hijack for their profit different pathways of glycan synthesis. Thus, the Bo17 gene of Bovine herpesvirus 4 (BoHV-4) encodes a homologue of the cellular core 2 protein beta-1,6-N-acetylglucosaminyltransferase-mucin type (C2GnT-M), which is a key player for the synthesis of complex O-glycans. Surprisingly, we show in this study that, as opposed to what is observed for the cellular enzyme, two different mRNAs are encoded by the Bo17 gene of all available BoHV-4 strains. While the first one corresponds to the entire coding sequence of the Bo17 gene, the ...
TopThis module aims at providing an in-depth knowledge in the field of host-pathogen interactions, i.e how the immune system deals with pathogens, and how the pathogens deal with the hosts immune system. An introductory lecture series covers the basics in microbiology (bacteriology, virology, parasitology), immunology, vaccinology, and general principles of host-pathogen interactions. Selected diseases illustrate host-pathogens interactions along with the consequences for vaccine and drug design. The following set of lectures covered by clinicians and professionals focus on patient management, field study, as well as safety aspects when working with pathogens in a research lab. Tutorials are broken into "journal club", "article write-up exercise" and "problem-based study" and are directly related to the topics developed during the lectures ...
Infectious diseases continue to pose significant risks to human, animal and plant health. A better understanding of these threats - and the mechanisms that host cells mount to fend off the infections - is key to the diagnosing, treating and preventing the spread of disease.
Characterize at the biochemical level the molecules involved in these processes, define the way they interact and study their tridimensional structure by X-ray crystallography.
Interactions between pathogens and host immune cells are complex, dynamic, and play a critical role in disease pathogenesis. The outcome of such interactions is a reflection of the properties of the microbial agent and the ability of the host to respond to infection. This outcome may range from disease, persistent infection within the host, or even microbe elimination. Pathogenic organisms and/or their virulence factors often exploit the host immune machinery and subsequently modulate cell function, signaling, migration, and cytoskeleton rearrangement. An emerging theme in microbe-host interaction is that evolutionarily distant pathogens exploit a number of common cellular pathways. Understanding the common cellular targets and immune dysfunction elicited by various microbes may offer critical intervention to deregulate functional immunity by disseminating the impact of invading microbes and leading to novel therapeutic strategies.. Our research focuses on studying host-pathogen interactions ...
A new report appearing in the March 2014 issue of The FASEB Journal helps shed light on what drives the evolution of pathogens, as well as how our bodies adapt to ward them off. Specifically, the report shows that our bodies ...
CELL BIOLOGY. Breaking down miRNAs. Elbarbary et al. identify an endonuclease that initiates decay of microRNAs that regulate the cell cycle.. PROTEOMICS. Mapping the proteome. Thul et al. generated the Cell Atlas, an image-based collection of 12,003 proteins and 13 organelles that reveals proteins that exhibit multiple localizations and single-cell variation (see also Horwitz and Johnson).. HOST-MICROBE INTERACTIONS. Taking a look at plant-microbe relationships. Martin et al. review the evolution of plant-symbiont interactions that modulate plant signaling pathways, development, and immunity. ...
PhD Research Project: Using live-cell imaging of the immune response to investigate host-pathogen i in £30,000 - £39,999, Academic (e.g. Lecturer), Science with Global Academy Jobs. Apply Today.
Research on host-microbe interactions has become an emerging cross-disciplinary field. In both vertebrates as well as invertebrates a complex microbiome confers immunological, metabolic and behavioural benefits; its disturbance can contribute to the development of disease states. However, the molecular and cellular mechanisms controlling interactions within the metaorganisms are poorly understood and many key interactions between the associated organisms remain unknown.. We are particularly interested in:. ...
The Function Pillar is devoted to exploration at the molecular level of host-microbe interactions and signalling relevant to health. It should open avenues to developing new bioactive compounds, targeting in particular immunomodulation and cell proliferation and also rational modulation of the microbiota. ...
Brunham, R C, F A Plummer, and R S Stephens. "Bacterial antigenic variation, host immune response, and pathogen-host coevolution.." Infection and Immunity 61.6 (1993): 2273-2276. Web. 19 Jan. 2020. ...
Many viruses evade host defense mechanisms by targeting specific host vulnerabilities, revealing critical points in host pathways regulating antiviral responses...
This theme emphasises quantitative approaches to understanding the pathogenesis and transmission of infectious diseases and involves epidemiology, genetics and evolution, and mathematical biology. It includes the epidemiology of endemic and exotic pathogens, mathematical modelling of host-pathogen interactions, and the informatics of pathogen evolution. This themes also investigating the epidemiology, disease burden and interactions of zoonotic pathogens - those that are transmitted between animals and humans. This research is analysing emergence of zoonotic diseases, quantifying their burden on populations and designing appropriate interventions, through strong collaborative links in tropical countries, in particular with sub-Saharan Africa.. ...
Keywords: HIV; Herpes; Host Factors; Host-Pathogen Interactions; Innate Immunity; Infection; Viruses; Restriction; Cyclophilin; TRIM5; Tetherin; Tropism; Gene ...
In this highly collaborative project, I was initially challenged to establish a working protocol for visualising colonisation in tiny tissue samples from the EHPC model. We spent time optimising the assay in vitro. Then, as the first volunteers of the study arrived, I processed the samples and tested the protocol for real. It took a total of 75 intense hours to manually scan all the microscope slides, searching for images to capture. There were some beautiful cell images, worth the patience (see below). Every single bacterium I found induced an adrenaline rush and was imaged for the record. The protocol also works for natural carriage and so opens avenues for exploring host-pathogen interactions in vivo, assessing markers of immune activation, identifying receptor-ligand interactions and investigating co-infection by different bacterial types. We then had to find a way to effectively study the host response to colonisation and have generated a novel RNAseq pipeline using transcriptomic data from ...
Get an introduction to modern medical virology, with an emphasis on structure, molecular biology, viral replication, mutations, evolution of viruses, host cell interactions and pathogenesis, as well as diagnosis, control and prevention of infection.
My research focus is to develop and apply computational and statistical methods to study host-pathogen interactions in barley and maize. I am currently working on microarray and RNA-Seq analysis for a barley-powdery mildew infectome. ...
Sassan Asgari is a PeerJ user. Bio: Interested in insect host-pathogen interactions and the role of microRNAs in the interactions
Topics: Structure and function of synaptic proteins; neurotoxins and receptors; host-pathogen interaction; protein complexes; protein-protein and protein-ligand interactions; X-ray ...
09:33, 6 April 2010 diff hist +158‎ N Amanda N. Wavrin Week 10 ‎ New page: ==Vibrio cholerae Journal Club Article== ===Host-induced Epidemic Spread of the Choler Bacterium== ====Vocabulary==== #hyperinfectious # # # # # # # # # current ...
Study Flashcards On Blood Borne Pathogens at Cram.com. Quickly memorize the terms, phrases and much more. Cram.com makes it easy to get the grade you want!
gain entry to a host. -colonise the tissue of the host. -resist the defences of the host. -cause damage to the host tissues. pathogens include bacteria, virus and fungi.. if a pathogen gets into a host and colonises its tissue an infection results. disease occurs when an infection leads to recognisable symptoms in the host. when a pathogen is transferred from one individual to another it is known as transmission. ...
வருகிற ஜனவரி 8ஆம் தேதி ராகு கேது பெயர்ச்சி நடக்கிறது.இப்போது கன்னி ராசியில் இருக்கும் ராகு சிம்மம் ராசிக்கு வருகிறார் .கேது மீனம் ராசியில் இருந்து கும்பம் ராசிக்கு பெயர்ச்சியாகிறார்..ராகு கேது பின்னோக்கிதான் பெயர்ச்சியாவார்கள்…8.1.2016 முதல் 27.7.2017 வரை யான பலன்கள் அனைத்து ராசியினருக்கும் கொடுத்துள்ளேன்.. ராகுவை போல கொடுப்பார் இல்லை.கேதுவை போல கெடுப்பார் இல்லை என்பார்கள் ...
ISSN 225-1448. This archival website is powered by Pampa 5.1 and hosted by the Office of Population Research at Princeton University. For website issues contact us at [email protected] For all other matters please contact IUSSP at [email protected] ...
Pectin lyases (PNLs) are important enzymes that are involved in plant cell wall degradation during the infection process. Colletotrichum is a diverse genus of fungi, which allows the study of the evolution of PNLs and their possible role in pathogen-host interactions and lifestyle adaptations. The phylogenetic reconstruction of PNLs from Colletotrichum and analysis of selection pressures showed the formation of protein lineages by groups of species with different selection pressures and specific patterns. The analysis of positive selection at individual sites using different methods allowed for the identification of three codons with evidence of positive selection in the oligosaccharide-binding region and two codons on the antiparallel sheet, which may influence the interaction with the substrate ...
Phage display has become an established, widely used method for selection of peptides, antibodies or alternative scaffolds. The use of phage display for the selection of antigens from genomic or cDNA libraries of pathogens which is an alternative to the classical way of identifying immunogenic proteins is not well-known. In recent years several new applications for oligopeptide phage display in disease related fields have been developed which has led to the identification of various new antigens. These novel identified immunogenic proteins provide new insights into host pathogen interactions and can be used for the development of new diagnostic tests and vaccines. In this review we focus on the M13 oligopeptide phage display system for pathogen research but will also give examples for lambda phage display and for applications in other disease related fields. In addition, a detailed technical work flow for the identification of immunogenic oligopeptides using the pHORF system is given. The described
Subject: Lambda Lunch update To: [email protected] Lambda Lunch update, 1/29/08: 1/29/08, Noon, Building 32TII Conference Room: Emily Troemel (Fred Ausubel lab, MGH) "Host/pathogen interactions in C. elegans" 1/29/08, 2:15 PM, 37/2041: Heather W. Pinkett, (Caltech) "Structural Studies of an ABC Transporter from Haemophilus influenzae, HIF" 1/31/08*: Bob Schleif (Hopkins) "Pursuing a Proteins Innermost Secrets: AraC" (Sankar Adhya) 2/7/08*: Christophe Penno (Cashel lab) "Control of the virulence of Shigella flexneri by transcriptional slippage" 2/8/08, Noon, Building 32TII Conference Room: Jennifer Philips (Perrimon + Rubin labs, Harvard Med) "Genome-wide RNAi screen in Drosophila identifies host factors that modulate Mycobacterial infection" 2/8/08, 9 AM - 12 PM, Natcher (Building 45) Room E1/E2: Imaging Bacterial Structures: A Symposium in Honor of Martin Kessel. Register at http://www.csmicro.org/html/CSM_Winter08_Symposium.htm 3/7/08: 12:00 PM, 32TII/Conf. room: Petra Levin (Wash ...
Researchers at the Institute of Medical Microbiology and Hygiene aim to elucidate host pathogen interactions to provide a basis for the development of new strategies to fight infectious diseases. Current research at the Institute has evolved over decades: the nineteen-sixties through nineteen-eighties, under the leadership of P. Klein, heralded an era of pioneering studies on complement, interleukins and T-cells, with bacterial virulence factors and molecular biology of viruses emerging as additional themes in the nineteen-eighties. From 1990 until early 2012 the Institute was led by S. Bhakdi, whose research focused on bacterial pore forming toxins and complement. During these two periods, the Institute also served as an important coordinator of Collaborative Research Centers in the field of Immunology. Between September 2013 and October 2016, A. Diefenbach held the position as director of the Institute; and a new class of lymphoid cells became the main area of investigation. Thus, for more ...