TY - JOUR. T1 - Separation and analysis of subcellular organelles in a human promyelocytic leukemia cell line, HL-60. T2 - Application to the study of myeloid lysosomal enzyme synthesis and processing. AU - Nauseef, W. M.. AU - Clark, Robert A. PY - 1986. Y1 - 1986. N2 - We describe a system for analysis of the intracellular pathways in the biosynthesis and packaging of functionally important proteins in human myeloid cells. The human promyelocytic cell line HL-60 was used since peripheral blood neutrophils are terminally differentiated and do not actively synthesize protein. Cells were disrupted by nitrogen cavitation and subcellular organelles in postnuclear supernatant separated on a discontinuous gradient of Percoll modified to resolve organelles important in protein synthesis. This Percoll gradient separated azurophilic granules from less dense organelles and partially separated the less dense organelles from one another. Approximate densities of organelles identified by electron microscopy ...

The present study selected and characterized a multidrug‑resistant HL‑60 human acute promyelocytic leukemia cell line, HL‑60/RS, by exposure to stepwise incremental doses of doxorubicin. The drug‑resistant HL‑60/RS cells exhibited 85.68‑fold resistance to doxorubicin and were cross‑resistant to other chemotherapeutics, including cisplatin, daunorubicin, cytarabine, vincristine and etoposide. The cells over‑expressed the transporters P‑glycoprotein, multidrug‑resistance‑related protein 1 and breast‑cancer‑resistance protein, encoded by the adenosine triphosphate‑binding cassette (ABC)B1, ABCC1 and ABCG2 genes, respectively. Unlike other recognized chemoresistant leukemia cell lines, HL‑60/RS cells were also strongly cross‑resistant to arsenic trioxide. The proportion of leukemia stem cells (LSCs) increased synchronously with increased of drug resistance in the doxorubicin‑induced HL‑60 cell population. The present study confirmed that doxorubicin‑induced ...

TY - JOUR AU - Jakovljević, Katarina AU - Joksovic, Milan D. AU - Matić, Ivana Z. AU - Petrovic, Nina AU - Stanojković, Tatjana AU - Sladić, Dušan AU - Vujčić, Miroslava AU - Janović, Barbara AU - Joksovic, Ljubinka AU - Trifunović, Snežana AU - Markovic, Violeta PY - 2018 UR - http://cer.ihtm.bg.ac.rs/handle/123456789/2379 AB - Hybrid compounds that combine the 1,3,4-thiadiazole-containing catechol moiety with a chalcone motif were synthesized and examined for their antioxidant activity, cytotoxicity, and DNA-binding activity. A series of thirteen compounds showed strong antioxidant and cytotoxic effects on human acute promyelocytic leukemia HL-60 cells. Several compounds exerted good cytotoxic activities on cervical adenocarcinoma HeLa cells. The treatment of HeLa cells with IC50 and double IC50 concentrations of the compounds 5a, 5c, 5f, and 5m induced a statistically significant increase in the percentage of cells within a subG1 cell cycle phase. The examined compounds caused G2/M ...

TY - JOUR. T1 - Analysis of gene profiles involved in the enhancement of all-trans retinoic acid-induced HL-60 cell differentiation by sesquiterpene lactones identifies asparagine synthetase as a novel target for differentiation-inducing therapy. AU - Song, Ju Han. AU - Kim, Seung Hyun. AU - Cho, Kyung Min. AU - Hwang, Seung Yong. AU - Kim, Hyeoung Joon. AU - Kim, Tae Sung. PY - 2014/3/1. Y1 - 2014/3/1. N2 - All-trans retinoic acid (ATRA) is one of the most useful drugs in the treatment for acute promyelocytic leukemia (APL), but its adverse effects, which include drug resistance and hypercalcemia are obstacles to achieving complete remission. Our previous study showed that some sesquiterpene lactones (STLs), i.e., helenalin (HE) and parthenolide (PA) but not sclareolide (SC), enhance ATRA-induced differentiation of HL-60 APL cells with no unexpected effects, but the precise mechanism on underlying this synergism is not yet fully understood. In this study, we investigated the distinctive ...

The mechanism of neutrophil activation by the chemotactic peptide formyl-methionyl-leucyl-phenylalanine (FMLP) has been studied by pretreatment of human neutrophils with pertussis toxin. Upon stimulation with FMLP, the cytosolic-free calcium concentration, [Ca2+]i, is increased both by stimulation of calcium influx and mobilization of cellular calcium. We have measured [Ca2+]i as well as the generation of the phospholipid breakdown product inositol trisphosphate (IP3), which is thought to mediate Ca2+ mobilization. As the phosphoinositide pool in human neutrophils is difficult to prelabel with [3H]myoinositol, experiments were also carried out in the cultured human promyelocytic leukemia cell line HL-60 after differentiation with dimethylsulfoxide. Pertussis toxin pretreatment of both cell types inhibited FMLP stimulated membrane depolarization, exocytosis, and superoxide production in a dose-dependent manner. This toxin effect was selective for the receptor agonist, since stimulation of these ...

Anti-leukemic activity of phosphoproteins from Sesamin via induction of nuclear antigen H731and CLIP-associating protein 2 isoform X25 mediated apoptosis

TC Hsieh, J Kunichki, Z Darzynkiewicz, JM Wu.. Department of Biochemistry and Molecular Biology, New York Medical College, Valhalla, NY, USA.. OBJECTIVE: The goal of this in vitro study was to test the cytostatic and cytotoxic activities of extracts derived from the polysaccharopeptide (PSP), Im-Yunity (Integrated Chinese Medicine Holdings Ltd., Kowloon, Hong Kong) prepared from strain Cov-1 of the mushroom Coriolus versicolor. DESIGN: Different volumes of 70% ethanol and water extracts of Im-Yunity were incubated with cultures of human promyelocytic leukemic HL-60 cells, and compared to nontreated control cells. At various times after treatment, cells were harvested and analyzed with respect to: (1). proliferation and cell cycle phase distribution, (2). induction of apoptosis, and (3). changes in expression of the immunomodulating cytokines interleukin (IL)-1 beta, IL-6, and IL-8. To test whether extracts also affected normal cells, similar experiments were also performed using isolated ...

Treatment of intact NIH 3T3 cells with 12-O-tetradecanoylphorbol-13-acetate (TPA) causes a rapid redistribution (stabilization) of protein kinase C to the particulate fraction. Part of the enzyme activity stabilized to the membrane fraction in response to TPA can be recovered associated with nuclear-cytoskeletal components. An apparently pure nuclear fraction prepared from NIH 3T3 cells was found to contain 25-30% of the total membrane-associated protein kinase C activity when isolated in the presence of Ca2+. In untreated control cells, most of this activity found with the nuclear fraction can be extracted by chelators. Phorbol easter (TPA) treatment of NIH 3T3 cells induces the tight association of protein kinase C to the nucleus; this tightly bound activity is not dissociable by chelators and can be recovered only by solubilization with detergent. Nuclei purified from untreated human promyelocytic leukemic HL-60 cells contain higher amounts of chelator-stable, detergent-extractable protein ...

Sigma-Aldrich offers abstracts and full-text articles by [Elisabetta Cavalieri, Antonella Rigo, Massimiliano Bonifacio, Alessandra Carcereri de Prati, Emanuele Guardalben, Christian Bergamini, Romana Fato, Giovanni Pizzolo, Hisanori Suzuki, Fabrizio Vinante].

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TY - JOUR. T1 - Soluble CD141-152 confers responsiveness to both lipoarabinomannan and lipopolysaccharide in a novel HL-60 cell bioassay. AU - Yu, Weiming. AU - Soprana, Elisa. AU - Cosentino, Giovanna. AU - Volta, Manuela. AU - Lichenstein, Henri S.. AU - Viale, Giovanna. AU - Vercelli, Donata. PY - 1998/10/15. Y1 - 1998/10/15. N2 - CD14 is a pattern recognition receptor involved in the interaction with multiple ligands, including LPS from Gram-negative bacteria and lipoarabinomannan (LAM) from mycobacteria. While the interactions between LPS and soluble CD14 (sCD14) have been analyzed in detail, LAM/CD14 interactions remain uncharacterized due to the lack of suitable functional assays. We describe herein a novel bioassay for the analysis of CD14/ligand interactions. CD14-negative myeloid HL-60 cells up-regulate endogenous CD14 gene expression when stimulated with LPS in the presence of recombinant soluble CD141-348. Using the HL-60 bioassay, we showed that sCD141- 348 confers responsiveness ...

While the molecular and biophysical mechanisms underlying cell protrusion on two-dimensional substrates are well understood, our knowledge of the actin structures driving protrusion in three-dimensional environments is poor, despite relevance to inflammation, development and cancer. Here we report that, during chemotactic migration through microchannels with 5 μm × 5 μm cross-sections, HL60 neutrophil-like cells assemble an actin-rich slab filling the whole channel cross-section at their front. This leading edge comprises two distinct F-actin networks: an adherent network that polymerizes perpendicular to cell-wall interfaces and a free network that grows from the free membrane at the cell front. Each network is polymerized by a distinct nucleator and, due to their geometrical arrangement, the networks interact mechanically. On the basis of our experimental data, we propose that, during interstitial migration, medial growth of the adherent network compresses the free network preventing its

HL-60 is a promyelocytic cell line derived by S.J. Collins, et al. Peripheral blood leukocytes were obtained by leukopheresis from a 36-year-old Caucasian female with acute promyelocytic leukemia.

Our findings suggest an inherent, cryptic chirality in VMCs that is revealed by an unbiased extracellular mechanical transition and mediated by cytoskeletal reorganization, analogous to chemically induced chirality seen in neutrophil-like cells.31 To our knowledge, this is the first demonstration of an association between LR asymmetry and cytoskeletal reorganization, triggered by an unbiased mechanical interface, and the first demonstration that a microscale dynamic asymmetry unfolds into a de novo, consistently oriented and periodic macroscale pattern resembling tissue architecture. In VMCs, the rightward-biased turning required stress-fiber accumulation at the FN/PEG interface, suggesting that chirality may be in the architecture of the actin filament assembly at the macroscale level, say as clockwise or counterclockwise orientation. Alternatively, it may arise from chirality at the micro- or nanoscale, such as helicity of microfilaments, or chiral rotagen molecules, such as dynein or myosin, ...

BioAssay record AID 43233 submitted by ChEMBL: The compound was evaluated for antitumoral activity against human leukemia cell line CCRF CEM after 72 hr of incubation.

Cardiac structure and functionare commonly studied using primary culture of neonatal and adult cardiac myocyte. However, their inability to divide and retain their differentiated phenotype in culture limits their use.. Established from a mouse atrial myocyte tumour, HL-1 cells share similar characteristics with primary cultures of cardiac myocytes. They have the ability to proliferate while keeping a differentiated cardiomyocyte phenotype in culture (this allows the use of specific molecular tools as RNA interference). However, there are concerns about their genetic stability and some studies have shown the cells to contain a functionally heterogeneous population.. The team from Imperial College isolated homogeneous and stable clones of HL-1 cell lines - thereby excluding any differences due to cellular heterogeneity of the original cell line - that display phenotypic characteristics consistent with cardiac cells.. Clones 3 and 6 appear to be most promising for cardiac research. These cells ...

Janet E. Rubin (jrubi02 at unix.cc.emory.edu) wrote: : Can anyone help us with our RT-PCR problem? We have GAP : primers that work perfectly well in rat and mouse - and in fact were : suggested by a BioTechniques article because they were : supposed to read human as well. However, despite being able : to RT-PCR other things from HL-60 cells, our GAP primers do not : work, at all (no band). So the question is, is GAP wierd in HL-60 : cells (has anyone else RTd GAP without problems?), any : suggestions for good primers for housekeeping-ish genes in this : cell line? : Thanks in advance (or previously as someone once wrote). -- Shahram Mori _/\_ Program in Molecular Biology _\ /_ Dept. of chemistry and Biochemistry Box 3C \_ _/ NMSU Las Cruces NM ...

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Detailed information about the celline expression of DLST in HL-60 stained with HPA003010. The antibody showed a High level of staining

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In these studies we have identified a novel small molecular weight mimetic of TGF-β, A-161906. A-161906 was originally synthesized as part of a series of metalloproteinase inhibitors at Abbott Laboratories. A-161906 is a micromolar inhibitor of TNF-α release in PMA-stimulated HL-60 cells. PMA stimulates the release of TNF-α concomitant with the differentiation of HL-60 cells. However, in a lipopolysaccharide-stimulated model in a more monocytic-differentiated THP-1 cell line, A-161906 has no effect on TNF-α release. This suggested that A-161906 had other mechanisms aside from the inhibition of TNF-α release, a metalloproteinase-dependent process, that were associated with the differentiation of HL-60 cells by PMA. HTS of a PAI-1/luciferase construct in Mv1Lu cells provided the identification of a novel activity of A-161906, modulation of transcriptional promoter activity in a cellular assay sensitive to TGF-β (32).. In cancer and other diseases where the normal responses to TGF-β have ...

TY - JOUR. T1 - Ellagitannins from Terminalia calamansanai induced apoptosis in HL-60 cells. AU - Chen, Lih Geeng. AU - Huang, Wen Tsung. AU - Lee, Lain Tze. AU - Wang, Ching Chiung. PY - 2009/6. Y1 - 2009/6. N2 - Terminalia calamansanai (Blanco) Rolf. (Combretaceae) is used medicinally as lithontriptic in Philippines. The 70% acetone extracts of T. calamansanai leaves inhibited the viability of human promyelocytic leukemia HL-60 cells. 1-α-O-Galloylpunicalagin, punicalagin, 2-O-galloylpunicalin, sanguiin H-4, and methyl gallate were the main components isolated from T. calamansanai with the IC 50 values of 65.2, 74.8, 42.2, 38.0 and ,100 μM, respectively, for HL-60 cells. Apoptosis of HL-60 cells treated with 1-α-O-galloylpunicalagin, punicalagin, 2-O-galloylpunicalin, and sanguiin H-4 was noted by the appearance of a sub-G 1 peak in flow cytometric analysis and DNA fragmentation by gel electrophoresis. 2-O-Galloylpunicalin and sanguiin H-4 induced a decrease of the human ...

TY - JOUR. T1 - Dysregulated bcl-2 expression inhibits apoptosis but not differentiation of retinoic acid-induced HL-60 granulocytes. AU - Park, Ulie R.. AU - Robertson, Kent. AU - Hickstein, Dennis D.. AU - Tsai, Schickwann. AU - Hockenbery, David M.. AU - Collins, Steven J.. N1 - Copyright: Copyright 2020 Elsevier B.V., All rights reserved.. PY - 1994/7/15. Y1 - 1994/7/15. N2 - The bcl-2 proto-oncogene appears to contribute to the development of certain malignancies by inhibiting programmed cell death (apoptosis). Mature granulocytes show a markedly limited life span and rapidly undergo apoptosis. To further define the relationship between apoptosis and granulocyte differentiation, we used retroviral vector-mediated gene transduction to introduce the normal bcl-2 gene into the HL-60 myeloid leukemia cell line and determined the response of these bcl-2-transduced HL-60 cells to the induction of granulocyte differentiation by retinoic acid (RA). Although the bcl-2-transduced HL-60 cells showed ...

Mitogen-activated protein kinases (MAPKs) are important transducers of external signals for cell growth, survival, and other cellular responses including cell differentiation. Several MAPK cascades are known with the MEK1/2-ERK1/2, JNK, and p38MAPKs receiving most attention, but the role of MEK5-ERK5 in intracellular signaling deserves more scrutiny, as this pathway transmits signals that can complement ERK/2 signaling. We hypothesized that the ERK5 pathway plays a role in the control of monocytic differentiation, which is disturbed in myeloid leukemia. We therefore examined the cellular phenotype and key molecular events which occur when human myeloid leukemia cells, acute (AML) or chronic (CML), are forced to differentiate by vitamin D derivatives (VDDs). This study was performed using established cell lines HL60 and U937, and primary cultures of blasts from 10 patients with ML. We found that ERK5 and its direct downstream target transcription factor MEF2C are upregulated by 1,25D in parallel ...

The HL-60 (Human promyelocytic leukemia cells) cell line has been used for laboratory research on how certain kinds of blood cells are formed. HL-60 proliferates continuously in suspension culture in nutrient and antibiotic chemicals. The doubling time is about 36-48 hours. The cell line was derived from a 36-year-old woman with acute promyelocytic leukemia at MD Anderson Cancer Center. HL-60 cells are predominantly a neutrophilic promyelocyte (precursor). Proliferation of HL-60 cells occurs through the transferrin and insulin receptors, which are expressed on cell surface. The requirement for insulin and transferrin is absolute, as HL-60 proliferation immediately ceases if either of these compounds is removed from the serum-free culture media. With this line, differentiation to mature granulocytes can be induced by compounds such as dimethyl sulfoxide (DMSO), or retinoic acid. Other compounds like 1,25-dihydroxyvitamin D3, 12-O-tetradecanoylphorbol-13-acetate (TPA) and GM-CSF can induce HL-60 ...

Ultraviolet B (UVB) radiation acts as a strong apoptotic trigger in many cell types, in tumor and normal cells. Several studies have demonstrated that UVB-induced cell death occurs through the generation of reactive oxygen species. The consequent oxidative stress includes the impairment of cellular antioxidants, the induction of DNA damage and the occurrence of apoptosis. In this review, we investigated UVB apoptotic action in various cell models by using ultrastructural, molecular and cytofluorimetric techniques. Myeloid leukemia HL-60, T-lymphoblastoid Molt-4 and myelomonocytic U937 human cells, generally affected by apoptotic stimuli, were studied. Human chondrocytes and C2C12 skeletal muscle cells, known to be more resistant to damage, were also considered. All of them, when exposed to UVB radiation, revealed a number of characteristic apoptotic markers. Membrane blebbing, cytoplasm shrinkage and chromatin condensation were detected by means of electron microscopy. DNA cleavage, investigated by

The cytotoxic response of several types of neoplastic cells to analogues of unnatural alkyl phospholipids (e.g., rac-1-hexadecyl-2-methoxy-glycero-3-phosphocholine) has been partially attributed to their accumulation as a result of the low activity of the alkyl cleavage enzyme (a tetrahydropteridine-dependent monooxygenase) in tumor cells. We tested this possibility by comparing the alkyl cleavage enzyme activity in cells that exhibit differences in sensitivity toward the cytotoxic effects of the rac-1-hexadecyl-2-methoxy-glycero-3-phosphocholine. Human promyelocytic leukemia cells (HL-60), a cell line highly sensitive to the cytotoxic alkyl phospholipid analogue, possessed an alkyl cleavage enzyme activity (0.25 pmol/min/µg protein) similar to that found in three cell types known to be relatively resistant to the cytotoxic activity of the analogue: immature human promyeloblastic leukemia cells (K562) (0.22 pmol/min/µg protein), human polymorphonuclear neutrophils (0.34 pmol/min/µg protein), ...

Ortho-topolin riboside induced cell apoptosis through ERS pathway and inhibited DNMT1 activity in acute myeloid leukemia cells, Li Wang, YanHong Zhao, Jiao Cheng, FanLin Lin, YingY

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CD109 (Cluster of Differentiation 109) is a cell surface antigen that is linked to glycosyl-phosphatidyl-inositol (GPI). CD109 is also known as platelet-specific Gov antigen, 150 kDa TGF-beta-1-binding protein, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 7, CPAMD7, p180, r150, DKFZp762L1111, FLJ38569, and RP11-525G3.1. It is expressed by CD34+ acute myeloid leukemia cell lines, T-cell lines, activated T lymphoblasts, endothelial cells, and activated platelets. CD109 is considered to be a marker of early-stage megakaryocytic hematopoiesis. Overexpression of CD109 has been reported in squamous cell carcinomas, such as lung carcinoma, esophageal carcinoma, and cervical carcinoma.. ...

CD109 (Cluster of Differentiation 109) is a cell surface antigen that is linked to glycosyl-phosphatidyl-inositol (GPI). CD109 is also known as platelet-specific Gov antigen, 150 kDa TGF-beta-1-binding protein, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 7, CPAMD7, p180, r150, DKFZp762L1111, FLJ38569, and RP11-525G3.1. It is expressed by CD34+ acute myeloid leukemia cell lines, T-cell lines, activated T lymphoblasts, endothelial cells, and activated platelets. CD109 is considered to be a marker of early-stage megakaryocytic hematopoiesis. Overexpression of CD109 has been reported in squamous cell carcinomas, such as lung carcinoma, esophageal carcinoma, and cervical carcinoma.. ...

During 1.25% dimethylsulfoxide (DMSO)-triggered granulocytic differentiation of HL-60 cells, the neurotensin-induced [Ca2+]i rise became gradually smaller and completely disappeared 4 days after treatment with DMSO. The mRNA level for neurotensin receptors was also decreased after differentiation ...

BioAssay record AID 393286 submitted by ChEMBL: Inhibition of MPO activity in TNF-alpha-stimulated human HL60 cells measured enzyme activity per 106 cells at 50 uM by spectrophotometrically.

We first tested no matter whether unpolarized HL-60 neutrophils can break their morphological symmetry and start off to migrate when they are stimulated by a

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M induced an inhibitory effect against the proliferation of HL-60 and colony potential of HCT-116 cells. The apoptosis in HL-60 cells was associated with down-regulation of Bcl-2 and activation of Bax, while in HCT-116 cells, necrotic features were observed; size of cells was dramatically increased by swelling of cytoplasm and loss of membrane integrity, cell rupture and release of cellular contents. ...

Activation of ERK signaling may promote cardioprotection from ischemia-reperfusion (I/R) injury. ZnT-1, a protein that confers resistance from zinc toxicity, was found to interact with Raf-1 kinase through its C-terminal domain, leading to downstream activation of ERK. In the present study, we evaluated the effects of ZnT-1 in c...Read More ...

The Baseline and Classical controllers use a classic cascaded-loop architecture with three inner P-only loops to control the angular rates p,q,r, and three outer PI loops to control the angular positions phi,alpha,beta. The six proportional gains and three integral gains are all scheduled as a function of alpha and beta. The Baseline variant contains the baseline design featured in docid:aeroblks_ug.f4-50264. Parts 2 and 3 of this series use the Classical variant to walk through the tuning process. The active variant is controlled by the workspace variable CTYPE. Set its value to 2 to activate the Classical variant of the controller.. ...

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Effects of DCA treatment on p53mutated/ leukemic cell linesThe p53mutated B leukemic cell lines MAVER, MEC-1 and MEC-2, as well as the p53 HL-60 cells, were exp

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CGP049090: an inhibitor of WNT signaling, effectively induce apoptosis in acute myeloid leukemia cells; structure in first source

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Violacein, a pigment isolated from Chromobacterium violaceum, has been reported to have multiple biological activities including in vitro antitumor effects. Certain anticancer agents are known to induce apoptosis in human tumor cell lines. In this work, our aim was to investigate the effectiveness of violacein/β-cyclodextrin (β-CD)-containing systems to produce lethal effects in the human promyelocytic leukemia cell line HL60. Using the MTT tetrazolium reduction test, IC 50 for the inclusion complexes (1:1 and 1:2 violacein:β-CD molar ratios) and violacein alone were less than 1 μM. Violacein and violacein/β-CD complexes were able to induce NBT reduction. Moreover, by using the Feulgen reaction, all the compounds were found to trigger apoptosis in HL60 cells, inducing around 35% of DNA fragmentation, as analyzed through the diphenylamine assay. In addition, caspases seem to play an important role in the activation of the executioner phase of apoptosis induced by violacein and its ...

Plumbagin, a naphtoquinone from the roots of Plumbago zeylanica is known to possess anticancer and anti-bacterial activity. Based on the former finding of our group in vitro demonstrating its effectiveness in human promyelocytic leukemia cells, NB4, in this study we further revealed the mitochondrial pathway involved in plumbagin-induced apoptosis. We also found that the generation of ROS was a critical mediator in plumbagin-induced apoptosis, which would be abrogated completely by antioxidant, NAC. The anticancer effect of plumbagin was investigated in vivo using NB4 tumor xenograft in NOD/SCID mice. The incidence of formation, growth characteristics, body weight and volume of tumors were observed. The histopathologic examination of tumors and organs were made. The results showed that intraperitoneal injection of plumbagin (2 mg/kg body weight) daily for 3 weeks resulted to a 64.49% reduction of tumor volume compared with the control. Furthermore, there was no overt manifestation of toxicity ...

Hiperisin, sar kantaron (Hypericum perforatum L.) olarak bilinen bitkinin aktif bile enlerinden birisidir. Hiperisinin antit m ral zellikleri oldu u solid organ t m rlerinin h cre dizileri ve hayvan modellerinde g sterilmi ve bunun mitokondrial fonksiyonlarda bozulma neticesinde ortaya kt g sterilmi tir. In vitro antil semik etkisi de vard r. Ancak etki mekanizmas tam olarak ayd nlat labilmi de ildir. Hiperium ekstrakt ile hiperisine ait kar la t rmal sitotoksisite al mas yoktur ve sitotoksisitenin alt nda yatan mekanizma ayd nlat lamam t r. Bu al mada Ege B lgesinde yeti en sar kantaron otundan Hypericum perforatum ekstrakt n n elde edilip, bu ekstrakt n HL-60 l semik h cre dizisinde doz ve zamana ba ml sitotoksisitesi olup olmad n ve varsa bu sitotoksisik etkiyi hiperisinin sitotoksik zellikleri ile kar la t rmak ve bu etkinin alt nda yatan mekanizman n a klanmas ama lanm t r. Sar kantaron otu ekstrakt n n 1/1000, 1/5000, 1/10.000, 1/50.000 dil syonlar ile yap lan deneylerde, IC50 dozunun ...

Bloodstream form T. brucei (TC221) and human myeloid leukemia HL-60 cells were grown axenically as described previously (11).. For toxicity tests, cells were seeded into 24-well plates at appropriate densities (104 trypanosomes/ml; 5 × 104 HL-60 cells/ml) in 1 ml of medium containing various concentrations (10−4 to 10−12 M) of proteasome inhibitors dissolved in 100% dimethyl sulfoxide (DMSO). The controls contained DMSO alone. In all experiments, the final DMSO concentration was 1%, which had no effect on the cell growth (11). After 48 h of incubation, living cells were counted with a Neubauer hemocytometer. The control cell counts were 106 trypanosomes/ml and 5 × 105 HL-60 cells/ml. Each experiment was set up in duplicate and repeated three times.. For detection of apoptosis, cells were exposed to proteasome inhibitors at various concentrations for 24 h, harvested by centrifugation, and fixed overnight with 70% ethanol at −20°C. Then, cells were washed twice with HBSS (Hanks balanced ...

TY - JOUR. T1 - Oncogene-dependent engraftment of human myeloid leukemia cells in immunosuppressed mice. AU - Kiser, M.. AU - McCubrey, J. A.. AU - Steelman, L. S.. AU - Shelton, J. G.. AU - Ramage, J.. AU - Alexander, R. L.. AU - Kucera, G. L.. AU - Pettenati, M.. AU - Willingham, M. C.. AU - Miller, M. S.. AU - Frankel, A. E.. N1 - Funding Information: This work was supported in part by the Leukemia and Lymphoma Society Grant No. 6114-99 (AEF), NIH CA76178 (AEF), NIH CA51025 (JAM) and the American Cancer Society Grant No. IRG-93-035-6 (GLK). The authors acknowledge the gift of SC-65461 and SC-50431 IL3 receptor agonist proteins from Dr Barbara Klein and Pharmacia, Inc. We thank Dr Douglas Case for input on statistical analyses.. PY - 2001. Y1 - 2001. N2 - We have developed an in vivo model of differentiated human acute myeloid leukemia (AML) by retroviral infection of the cytokine-dependent AML cell line TF-1 with the v-Src oncogene. When injected either intravenously or intraperitoneally into ...

Description: Using deep sequencing (deepCAGE), the FANTOM4 study measured the genome-wide dynamics of transcription-start-site usage in the human monocytic cell line THP-1 throughout a time course of growth arrest and differentiation. Modeling the expression dynamics in terms of predicted cis-regulatory sites, we identified the key transcription regulators, their time-dependent activities and target genes. Systematic siRNA knockdown of 52 transcription factors confirmed the roles of individual factors in the regulatory network. Our results indicate that cellular states are constrained by complex networks involving both positive and negative regulatory interactions among substantial numbers of transcription factors and that no single transcription factor is both necessary and sufficient to drive the differentiation process. ...

Using deep sequencing (deepCAGE), the FANTOM4 study measured the genome-wide dynamics of transcription-start-site usage in the human monocytic cell line THP-1 throughout a time course of growth arrest and differentiation. Modeling the expression dynamics in terms of predicted cis-regulatory sites, we identified the key transcription regulators, their time-dependent activities and target genes. Systematic siRNA knockdown of 52 transcription factors confirmed the roles of individual factors in the regulatory network. Our results indicate that cellular states are constrained by complex networks involving both positive and negative regulatory interactions among substantial numbers of transcription factors and that no single transcription factor is both necessary and sufficient to drive the differentiation process ...

HEPG2SP (hepatoblastoma carcinoma derived cell line secreted proteins): Hundred ml of supernatant HEPG2 culture media were concentrated down to 100 l in a MicrosepTM Concentrators. The concentrated sample was mixed with 400 l of a solution containing urea (8 M), CHAPS (4% w/v), DTE (65 mM), Resolytes 3.5-10 (2 % v/v) and a trace of bromophenol blue. The whole final diluted HEPG2SP sample was used for in-gel sample rehydration. NAME=1001363,HL60 (promyelocytic leukemia cells): A monolayer culture of a human promyelocytic leukemia cell line was grown in Dulbeccos modified Eagle medium (DMEM) containing 10% fetal calf serum (FCS). Cells were rinsed once with DMEM without FCS and removed from the flask by incubating them with a solution containing trypsin (0.5 g/l) and EDTA (0.2 g/l). After 3 minutes, DMEM containing FCS was added into the flask to stop the action of the trypsin. The cells were detached from the surface of the flask by squirting the solution onto the cells. The suspension was ...

Glaucocalyxin A (GLA) is a biologically active ent-kauranoid diterpenoid isolated from Rabdosia japonica var. glaucocalyx, a traditional Chinese medicinal herb, which has been shown to inhibit tumor cell proliferation. However, the mechanism underlying GLA-induced cytotoxicity remains unclear. In th …

TY - JOUR. T1 - Proteomic analysis of the response to cell cycle arrests in human myeloid leukemia cells. AU - Ly, Tony. AU - Endo, Aki. AU - Lamond, Angus I.. N1 - Wellcome Trust 083524/Z/07/Z, 097945/B/11/Z, 073980/Z/03/Z, 08136/Z/03/Z, and 0909444/Z/09/Z Angus I Lamond; European Research Council HEALTH-F4-2010-257082 Angus I Lamond; Biotechnology and Biological Sciences Research Council BB/K003801/1 Angus I Lamond.. PY - 2015/1/2. Y1 - 2015/1/2. N2 - Previously, we analyzed protein abundance changes across a minimally perturbed cell cycle by using centrifugal elutriation to differentially enrich distinct cell cycle phases in human NB4 cells (Ly et al., 2014). In this study, we compare data from elutriated cells with NB4 cells arrested at comparable phases using serum starvation, hydroxyurea, or RO-3306. While elutriated and arrested cells have similar patterns of DNA content and cyclin expression, a large fraction of the proteome changes detected in arrested cells are found to reflect ...

TY - JOUR. T1 - Differential apoptosis-inducing effect of quercetin and its glycosides in human promyeloleukemic HL-60 cells by alternative activation of the caspase 3 cascade. AU - Shen, Shing Chuan. AU - Chen, Yen Chou. AU - Hsu, Feng-Lin. AU - Lee, Woan Rouh. PY - 2003/8/1. Y1 - 2003/8/1. N2 - Flavonoids were demonstrated to possess several biological effects including antitumor, antioxidant, and anti-inflammatory activities in our previous studies. However, the effect of glycosylation on their biological functions is still undefined. In the present study, the apoptosis-inducing activities of three structure-related flavonoids including aglycone quercetin (QUE), and glycone rutin (RUT; QUE-3-O-rutinoside), and glycone quercitrin (QUI; QUE-3-O-rhamnoside) were studied. Both RUT and QUI are QUE glycosides, and possess rutinose and rhamnose at the C3 position of QUE, respectively. Results of the MTT assay showed that QUE, but not RUT and QUI, exhibits significant cytotoxic effect on HL-60 cells, ...

Proprietary Mushroom Blend. There are an estimated 140,000 mushroom species on Earth, yet there are only 14,000 named species know. These comprise a vast and largely untapped source of powerful pharmaceutical products. In particular, they represent an unlimited source of polysaccharides with antitumor and immunostimulating properties. Mushroom polysaccharides prevent oncogenesis, show direct antitumor activity against various allogeneic and syngeneic tumors, and prevent tumor metastasis. Polysaccharides from mushrooms do not attack cancer cells directly, but produce their antitumor effects by activating different immune responses in the host. (Reference 1). Ganoderma lucidum. The antiproliferative activities on tumoral cells, namely, human breast cancer (MCF-7 and MDA-MB-231), hepatoma (HepG2) and myeloid leukemia (HL-60), of ethanolic extracts from two species of Ganoderma, G. lucidum and G. sinense, were investigated. Both had apoptosis induction through the alternation of mitochondrial ...

B36 Lantadene A (LA, 22β-angeloyloxy-3-oxoolean-12-en-28-oic acid) a pentacyclic triterpenoid isolated from leaves of obnoxious weed Lantana camara L. was evaluated for apoptosis induction in human leukemia HL-60 cell line. The effect of LA on cell proliferation of HL-60 cancer cells were determined by MTT assay. The morphological effects of LA treated HL-60 cancer cells were observed under a fluorescence microscope. DNA fragmentation was observed using gel electrophoresis. Flow cytometry was carried out to observe changes in cell-cycle distribution of the cells. The expression of Bcl-2 and Bax proteins in HL-60 cells were visualized by means of immunohistochemical assay and cell viability was determined upon treatment with DEVD-CHO and LA. Typical morphological changes including cell shrinkage, chromatin condensation and characteristic DNA ladder formation in agarose gel electrophoresis were observed. LA induced marked concentration and time dependant inhibition of cancer cell proliferation ...

Almost one-third of patients with acute myeloid leukemia have FMS-like tyrosine kinase 3 mutations and therefore have poor survival prospects. 3 with inner tandem duplication mutant murine xenograft model. Mice given a diet filled with ONO-7475 exhibited considerably longer success and, interestingly, obstructed leukemia cell infiltration in the liver organ. In conclusion, ONO-7475 effectively eliminates severe myeloid leukemia cells and by systems that involve disruption of different success and proliferation pathways. Launch Anexelekto (AXL) is normally a receptor tyrosine kinase (RTK) from the TAM (Tyro3, AXL, and MER) family members.1C4 Activation of AXL by growth arrest particular 6 (GAS6) induces diverse success cascades.1C4 Recent research have uncovered that AXL regulates survival signaling in buy PF-543 lots of cancers, including leukemia.1C5 High expression of AXL or GAS6 in AML patients is prognostic for poor survival outcome.6C10 The AXL/GAS6 axis promotes leukemia cell proliferation ...

|i|Artemisia|/i| is an important genus of Iranian flora. Cytotoxic activities for some species of the genus have already been reported. In this study, we have investigated the cytotoxic effects of |i|n|/i|-hexane, CH|sub|2|/sub|Cl|sub|2|/sub|, EtOAc, EtOH, and EtOH/H|sub|2|/sub|O (1 : 1) extracts of |i|A. turanica|/i| Krasch. on two human leukemic cancer cell lines (K562 and HL-60) and J774 as normal cells using alamarBlue (resazurin) assay. PI staining of the fragmented DNA and western blot analysis were used to evaluate the possible apoptotic effect of the extract. The CH|sub|2|/sub|Cl|sub|2|/sub| extract of |i|A. turanica|/i| showed the most antiproliferative effect on cancer cells among all tested extracts with IC|sub|50|/sub| values of 69 and 104 |i|μ|/i|g/mL on K562 and HL-60 cells, respectively, whereas the normal cells were not affected significantly by this extract. Sub-G1 peak in the flow cytometry histogram of the cells treated with CH|sub|2|/sub|Cl|sub|2|/sub|

The synthesis of a variety of 1-aryl-7-phenylaminoisoquinolinequinones from 1,4-benzoquinone and arylaldehydes via the respective 1-arylisoquinolinequinones is reported. The cyclic voltammograms of the new compounds exhibit two one-electron reduction waves to the corresponding radical-anion and dianion and two quasi-reversible oxidation peaks. The half-wave potential values (EI½) of the members of the series have proven sensitive to the electron-donor effect of the aryl group (phenyl, 2-thienyl, 2-furyl) at the 1-position as well as to the phenylamino groups (anilino, p-anisidino) at the 7-position. The antiproliferative activity of the new compounds was evaluated in vitro using the MTT colorimetric method against one normal cell line (MRC-5 lung fibroblasts) and two human cancer cell lines: AGS human gastric adenocarcinoma and HL-60 human promyelocytic leukemia cells in 72-h drug exposure assays. Among the series, compounds 5a, 5b, 5g, 5h, 6a and 6d exhibited interesting antiproliferative activities

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University of Alabama, Birmingham, Department of Medicine: Steffen Gay, MD (principal investigator); Renate E. Gay, MD; and Guoquiang Huang, MD (HL-33733). Department of Biochemistry: Edward J. Miller, PhD (principal investigator); Donald K. Furuto, PhD; Margaret S. Vail; and Annie J. Narkates (HL-33728).. Albany Medical College, NY: Assad Daoud, MD (principal investigator); Adriene S. Frank, PhD; Mary A. Hyer; and E. Carol McGovern (HL-33765).. Baylor College of Medicine, Houston, Tex: Louis C. Smith, PhD (principal investigator), and Faith M. Strickland, PhD (HL-33750).. University of Chicago, Ill: Robert W. Wissler, PhD, MD (principal investigator); Dragoslava Vessellinovitch, DVM, MS; Akio Komatsu, MD, PhD; Yoshiaki Kusumi, MD; Gregory M. Culen, DPM; Alyna Chien, BA; Alexis Demopoulos, BA; Gertrud Friedman, BA; R. Timothy Bridenstein, MS; Robert J. Stein, MD; Robert H. Kirschner, MD; Manuela Bekermeier, ASCP; Blanche Berger, ASCP; and Laura Hiltscher, ASCP (HL-33740).. University of ...

P2Y4 is a 365-amino acid 7 transmembrane protein. It has been shown that, when expressed in a mammalian cell line, the receptor protein was activated specifically by UTP and UDP, but not by ATP and ADP. Activation of P2RY4 resulted in increased inositol phosphate formation and calcium mobilization. Adrian et al, anazlyed the espression of several purinergic receptors during differentiation in a promyelocytic leukemia cell line. Granulocytic differentiation was induced by dimethylsulfoxide, and a monocytic/macrophage phenotype was induced by phorbol esters. P2RY4 was highly expressed in uninduced promyelocytes, and its expression decreased slightly following both granulocytic and monocytic differentiation.. ...

S100A8 and S100A9 are calcium-binding proteins predominantly expressed by neutrophils and monocytes and play key roles in both normal and pathological inflammation. Recently, both proteins were found to promote tumor progression through the establishment of premetastatic niches and inhibit antitumor immune responses. Although S100A8 and S100A9 have been studied in solid cancers, their functions in hematological malignancies remain poorly understood. However, S100A8 and S100A9 are highly expressed in acute myeloid leukemia (AML), and S100A8 expression has been linked to poor prognosis in AML. We identified a small subpopulation of cells expressing S100A8 and S100A9 in AML mouse models and primary human AML samples. In vitro and in vivo analyses revealed that S100A9 induces AML cell differentiation, whereas S100A8 prevents differentiation induced by S100A9 activity and maintains AML immature phenotype. Treatment with recombinant S100A9 proteins increased AML cell maturation, induced growth arrest, and

MCL1 Antibody is a Rabbit Polyclonal antibody against MCL1. This gene encodes an anti-apoptotic protein, which is a member of the Bcl-2 family. Alternative splicing results in multiple transcript variants. The longest gene product (isoform 1) enhances cel

Drug tolerant leukemic cell subpopulations may explain frequent relapses in acute myeloid leukemia (AML), suggesting that these Relapse-Initiating Cells (RICs) persistent after chemotherapy represent bona fide targets to prevent drug resistance and relapse. We uncovered that the G-protein coup...

Erufosine is an ether-lipid-derived synthetic compound belonging to the group of alkylphosphocholines (APCs). This chemotherapeutic agent exhibits pronounced anti-leukemic activity without affecting the normal hematopoiesis and exerts its influence by modulating signal transduction pathways in malignant tumor ...

1,25-dihydroxy-23-thiavitamin D3: shows differentiation-inducing activity of human myeloid leukemia cells into macrophages; structure given in first source

Thomas has had an exceptionally prolific and diverse career, making fundamental discoveries on human leukemia viruses, co-discovering the first human oncogenes, and revealing molecular regulatory mechanisms of cellular differentiation ad reprogramming. [more] ...

Unscramble antileukemic, Unscramble letters antileukemic, Point value for antileukemic, Word Decoder for antileukemic, Word generator using the letters antileukemic, Word Solver antileukemic, Possible Scrabble words with antileukemic, Anagram of antileukemic

Han SS, Kim K, Hahm ER, Lee SJ, Surh YJ, Park HK, Kim WS, Jung CW, Lee MH, Park K, Yang JH, Yoon SS, Riordan NH, Riordan HD, Kimler BF, Park CH, Lee JH, Park S. L-ascorbic acid represses constitutive activation of NF-kappaB and COX-2 expression in human acute myeloid leukemia, HL-60. J Cell Biochem. 2004 Oct 01; 93(2):257-70 ...

Xuehua Xu is the author of these articles in the Journal of Visualized Experiments: Imaging G-protein Coupled Receptor (GPCR)-mediated Signaling Events that Control Chemotaxis of Dictyostelium Discoideum, Imaging G Protein-coupled Receptor-mediated Chemotaxis and its Signaling Events in Neutrophil-like HL60 Cells

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A real-time Bonner sphere spectrometer (BSS) has been developed for spectral neutron measurements with the HL-2A Tokamak. To correct and verify the accuracy of the neutron spectrum from the BSS, the B ...