An adult patient with Sudan Black B (SB B) positive leukaemic lymphoblasts is described. Peroxidase and naphtol AS D chloroacetate esterase stains were negative. The diagnosis of acute lymphoblastic leukaemia (ALL) was based on morphology (FAB classification: L1), on immunological marker studies (cALL+, Tdt+, Ia+) and on electron microscopy, revealed blasts, compatible with lymphoblasts. Additional proof of the diagnosis of ALL were the diffuse lymphadenopathy and the rapid response to ALL chemotherapy. Scattered azurophilic granules were present in some lymphoblasts; ultrastructurally multiple lysosomal inclusions were detected, containing small vesicles, sometimes in association with entrapped cytoplasmic organelles. The large amounts of phospholipids in these inclusions explain the Sudan Black B positivity. This type of ALL can easily be misdiagnosed as acute myeloid leukaemia.
Semantic Scholar extracted view of Some histological and histochemical observations on the connective tissue of chronically inflamed human gingiva. by Andreas H. Melcher
DAB is the most commonly used amine substrate for the histochemical localization of peroxidases. DAB is an extremely sensitive indicator, producing a characteristic brown oxidation product easily visible with light microscopy. DAB can be used as a stand alone product, or it may be combined with KPL Enhance Black Buffer to create HistoMark BLACK substrate ...
IntroductionExperimental and clinical studies have shown that a normal, functioning endothelium is necessary in order to obtain successful penetrating keratopla
Cytological criteria for the diagnosis of acute myeloid leukaemia: French-American-British (FAB) classification FAB subtype MicroscopyCytochemistry/ Immunology M0 Blasts >30% of bone marrow nucleated cells <3% of blasts positive for Sudan Black B or POX Myeloperoxidase-positivity by ultrastructural cytochemistry Myeloid immunological markers (e.g. CD13, CD33, myeloperoxidase) M1 Blasts >90% of bone marrow nonerythroid cells > 3% of blasts positive for Sudan Black B or POX Maturing granulocytic cells (i.e. promyelocytes to PMN cells) < 10% non erythroid cells (Pro)monocytes < 10% non-erythroid cells
The architectural properties and fiber-type composition of the cat hip cuff muscles, i.e. the deep layer of muscles encircling the hip joint, were determined. The muscles studied included the gluteu
An Nervengewebskulturen menschlicher Foeten und neugeborener Ratten wurden Untersuchungen über die toxische Wirkung von Methylquecksilber (MMC) auf Neurone und Gliazellen im Rückenmark und Cerebellum...
Fig. (1) Localization of phloem unloading in the melon root tips. The emergence of the СF in the root tips in 0 (A), 1.5 (B), 4 (C) and 10 (D) hours after its application to the cotyledons. The dashed line indicates the boundary between the meristem and the elongation zone. The boundary between the root zones was determined by morphometric analysis. Bar - 500 µm. ...
Principal Investigator:KAWAKAMI Hayato, Project Period (FY):1995 - 1996, Research Category:Grant-in-Aid for Scientific Research (C), Section:一般, Research Field:General anatomy (including Histology/Embryology)
The Muscle Enzyme Histochemistry and IHC Lab is one of many pediatric labs at Nemours, providing muscle enzyme histochemistry and immunodiagnostics.
Migraine is also thought to be an inherited disorder giving rise to a "vulnerability" to an abnormal discharge of neurons (different from that seen in epilepsy) that preferentially affects brainstem regions and is triggered by a chemical event. Still working as good as the day they were bought. Para eliminar las arruguitas finas , rocephin and xarelto lo ideal es exfoliar la piel. In addition, rocephin and decadron shot side effects the Act strengthens federal laws, provides new sources of funding to assist victims of sexual assault and stalking, and provides a means for communities to build an effective coordinated community response to these crimes! Fine structural localization of a blood-brain barrier to exogenous peroxidase! Because the medication is delivered directly to the spinal cord, rocephin injection price your symptoms can be controlled with a much smaller dose than is needed with oral medication. Always consult your doctor about your medical conditions or back problem! All possible ...
The authors have explored the effect of intermittent hypoxia in the cell membrane and histochemical changes in the cardiac muscle cells of the white rat. Every day the animals were exposed to experimental conditions characteristic of 7.000 m of high above sea level (first experimental group) and every odd day (second experimental group). Animals of the experimental groups were sacrificed immediately (first subgroup), 24 hours later (second subgroup) and 7 days after the final exposition (third subgroup). The hypoxical stress elicited the vacuolation of the cardiac muscle fibres of the subendocardiac layer of miocard which persisted, and did not reduce its number, intensity and extensity in the first experimental group, although it reduced its intensity and vacuoles size in the second experimental group, while in fuxinorrhagia which has been presented focally the intensity has been reduced in all the three subgroups of the first experimental group of animals ...
ID PRSVADH preliminary; circular DNA; SYN; 6900 BP. XX AC ATCC77131; XX DT 01-JUL-1993 (Rel. 7, Created) DT 01-APR-1995 (Rel. 11, Last updated, Version 1) XX DE Vertebrate/E.coli phagemid vector pRSVADH - incomplete. XX KW cloning vector. XX OS Cloning vector OC Artificial sequences; Cloning vehicles. XX RN [1] RC pRSVADH from pWX008 & pRc/RSV RC pRSVPAP from pSV2Apap & pRc/RSV RC plasmid from pM-MuLV-SV-lacZ & pSK+ RC plasmid from plasmid & pECE RC pRSVlacZ from plasmid & pRc/RSV RC pCMVlacZ from pM-MuLV-SV-lacZ RC pCMVADH from pWX008 RA Lin W.C., Culp L.A.; RT "Selectable plasmid vectors with alternative and ultrasensitive RT histochemical marker genes"; RL Biotechniques 11:344-351(1991). XX CC Constructed by cloning the HindIII/XbaI fragment from pWX008 into CC pRc/RSV. [1] CC Transient expression experiments should be performed to examine the CC RSV promoter efficiency in different cell lines. The RSV promoter CC can be replaced by excising with a BglII/HindIII digest. (personal CC ...
小路 武彦 日本比較内分泌学会ニュース = Newsletter of Japan Society for Comparative Endocrinology 94, 19-23, 1999-08 J-STAGE 参考文献15件 ...
Introduction to website that provides information about cytochemistry and cell biology and links to Gwen Childs, Ph.D. and Microanatomy.net web sites.
Japans strongest earthquake on record has killed hundreds and raised fears about radioactive leaks from damaged nuclear power reactors. WSJs Jake Lee and Mariko Sanchanta, deputy Tokyo bureau chief,
TY - JOUR. T1 - Histochemical demonstration of membranous localization of endothelial nitric oxide synthase in endothelial cells of the rat brain. AU - Tomimoto, Hidekazu. AU - Akiguchi, Ichiro. AU - Wakita, Hideaki. AU - Nakamura, Sinichi. AU - Kimura, Jun. PY - 1994/12/19. Y1 - 1994/12/19. N2 - We examined cellular and subcellular localization of endothelial nitric oxide synthase by using immunoelectron microscopy. The results showed that immunoreaction products were associated with membranous structure, likely the endoplasmic reticulum and cytoplasmic vesicles. These membranous localization of imunoreactivities at electron microscopic level corresponded to the perinuclear granular structures observed by light microscopic immunohistochemistry for endothelial nitric oxide synthase as well as NADPH diaphorase histochemistry.. AB - We examined cellular and subcellular localization of endothelial nitric oxide synthase by using immunoelectron microscopy. The results showed that immunoreaction ...
Damjanov, I; Solter, D; and Skreb, N, "Enzyme histochemistry of experimental embryo-derived teratocarcinomas." (1971). Subject Strain Bibliography 1971. 1865 ...
Lipofuscin can make fluorescence imaging of human tissues virtually impossible. Lipofuscin consists of highly autofluorescent granules of oxidized proteins and lipids that build up in the lysosomes of aging cells in a variety of tissues. Lipofuscin granules fluoresce brightly in all channels used for fluorescence microscopy (Figure 1). Consequently, immunofluorescence in many human tissues or aged animal tissues can be virtually impossible unless lipofuscin fluorescence is masked.. TrueBlack® eliminates lipofuscin autofluorescence, clearing the way for immunofluorescence. Traditionally, Sudan Black B has been used to quench lipofuscin autofluorescence by incubating tissue sections with the dye after immunofluorescence staining. However, Sudan Black B also introduces non-specific red and far-red fluorescence, limiting the use of fluorescent dyes in those wavelengths. Biotium developed TrueBlack® as a superior alternative to Sudan Black B to quench autofluorescence with much lower background ...
Fig. (6) Staining of invertase activity on the parts of the transverse sections of a maize root tip from the region of the beginning of the elongation zone (A, B) and from the basal part of the meristem (C). The parts of the sections contain rhizodermis (rh), cortex (cor), endodermis (en), pericycle (pc) and stele (A, B) and a region of vascular cylinder (C). The reaction with (A, C) or without (В) sucrose in the incubation medium. Bar - 100 µm (A, B), 25 µm (C). ph - phloem, mx - metaxylem vessel, px - protoxylem vessel. ...
The directions of laboratory and clinical studies oriented to experimental confirmation of the priority concept of `laser histochemical surgery are presented. The goal of the studies is reproduction on experimental model of a number of pathologies (in vivo and in vitro) of the `sensitization to laser radiation by staining effect. Testing of the histochemical stains as sensitizers to laser irradiation of their `address substrates, i.e. vitally stained intracellular structures which participate in the pathologic processes evolution is under planning. The processes include: (a) metabolic disorders in the brain cells, i.e. disseminated sclerosis; (b) generalized metabolic disorders- -mucopolysaccharidosis and collagenosises (periarteritis nodosa, rheumatism, rheumatoid arthritis, sclerodermia); (3) metabolic disorders in individual organs--vessel atherosclerosis, hypercholesterolemia, myocardial infarction, cardiosclerosis, caries and parodontosis. The conditions of the studies are detailed in ...
This unit describes fixation and staining for b‐galactosidase activity; it has been successfully used on vertebrate embryos and tissue explants
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Wholesale Sulphur Black B - Select 2018 high quality Wholesale Sulphur Black B products in best price from certified Chinese Sulphur Black Pigment manufacturers, Sulphur Black Dyestuff suppliers, wholesalers and factory on Made-in-China.com
This paper describes some histologic and histochemical characteristics of an easily transplantable islet-cell tumor of the Syrian hamster. The presence of insulin was demonstrated by means of fluorescent antibody and immunoassay technics. A positive histochemical reaction for tryptophan suggests, but does not prove, the presence of small amounts of glucagon. It is suggested that the tumor may be a useful tool for biologic studies.. ...
Human lymphocytes, cultured in the presence of phytohemagglutinin, undergo morphologic transformation and subsequent mitosis. Before mitosis (48 to 72 hours), a sharp increase in acid phosphatase activity occurs in cells stimulated with phytohemagglutinin. Histochemical examination of these cells demonstrates that innumerable granules containing acid phosphatase develop in the cytoplasm before mitosis. It is possible that enzymes present in granules which stain for acid phosphatase activity (lysosome-like) may play a role in phytohemagglutinin-stimulated cell division. ...
fibrosis (es); Fibrosi (eu); fibrosi (ca); Fibrose (de); فیبروز (fa); 纤维化 (zh); 線維化 (ja); Fibros (sv); Фіброз (uk); Fibrosis (la); 섬유화 (ko); fibrozo (eo); Fibróza (cs); இழைநார்ப் பெருக்கம் (ta); Fibrosi (it); Fibrose (fr); Fibroza (hr); Xơ hóa (vi); Fibroza (sr); Fibrose (gl); לייפת (he); Fibrosis (id); Fibrosi (pl); fibrose (pt); Fibrose (nl); фіброз (be-tarask); Fibroza (bs); Фиброз (ru); Фиброза (bg); fibrosis (en); تليف (ar); Fibrosis (ms); Fibroz (uz) formation of excess fibrous connective tissue in an organ or tissue in a reparative or reactive process (en); Ehun bat zuntzezko ehun bilakatzean datzan fenomenoa (eu); formation of excess fibrous connective tissue in an organ or tissue in a reparative or reactive process (en) 線維症, 抗線維化, 抗線維化作用, 線維形成性 (ja); 纖維結締組織 (zh); التليف (ar ...
Alkaline Phosphatase. Sigma-Aldrich offers a broad range of alkaline phosphatase preparations optimized for conjugation to antibodies and other proteins for ELISA, Western blotting, and histochemical detection.
This kit is designed for histochemical detection of fragmented DNA using the TUNEL method. It allows quick and easy detection of single cells at the primary stage of apoptosis. Tissue sections and fixed cells may be used for samples.
This kit is designed for histochemical detection of fragmented DNA using the TUNEL method. It allows quick and easy detection of single cells at the primary stage of apoptosis. Tissue sections and fixed cells may be used for samples.
Thanks to Barry for this wonderful list. One reference, #4 Beckstead is actually about a formalin-free fixative, Zinc Tris Buffer with paraffin embedded tissues when CD markers are compromised by formalin or PFA fixation. Beckstead did this study for human CD markers in lymphomas. At 02:03 PM 6/23/2006, you wrote: >Some suggestions. >This is a short list. >Barry > >1. Mann, Gustav 1902. Physiological Histology. Methods and Theory. >Oxford at the Clarendon Press. This text deals in detail with methods in >histology and for fixation with the history and rationale behind using >various chemical agents as fixatives. > >2. Pearse A.G.E. The Chemistry and practice of Fixation. Chapter 5 >pages 97 -158. In Histochemistry Theoretical and Applied vol 1. 4th >edition. Preparative and Optical Techniques. 1980. Excellent description >of fixation chemistry and extensive list of references. vol. 2. >Analytical techniques 1985. vol 3. Enzyme Histochemistry 1991. Churchill >Livingstone, London. > >3. Arnold ...
Estrogen studies have largely focused on the use of biochemical and histochemical techniques to track these mechanisms," Dominguez said. What he has been able to do with his research is provide dramatic real-time visualizations of these mechanisms, particularly in live neurons.. The images for his study are generated by a custom-built microscope illuminated with an Argon laser, then captured by an Electron Multiplying Charge Coupled Device camera.. "Access to custom high-end equipment and advance training were initially what drew me to USC from UCLA as a postdoc," he said. "But its the mentorship that really make my postdoc experience incredible and sustainable.". In 2013, Dominguez was the recipient of the USC Provosts Postdoctoral Scholar Research Grant. The $25,000 award supported a recent paper in collaboration with Robert Chow, principal investigator at the Chow laboratory at USC, and Kassandra Kisler from the Berislav Zlokovic lab, who specializes in the study of Alzheimers disease ...
This concise book, by one of the worlds foremost liver pathologists, presents an up-to-date concept of liver pathology based on vast clinical experience at the Royal Free Hospital in London, as well as with patients sent from elsewhere. The text is well structured, readable, illustrations are clear and of excellent quality, and the index is good. Morphologic details are explained clearly and concisely. This edition illustrates how advances of histochemical procedures for the diagnosis of liver disease can be put to good use and updates the histologic features of different liver diseases discussed in previous editions. This book should be ...
Foram utilizados 15 peixes da espécie Centropomus parallelus conhecida como robalo peva provenientes da região estuarino-lagunar de Cananéia Estado de São Paulo. A análise morfológica foi realizada em preparações coradas pelo método de Rosenfeld e Laür. Para estudo citoquímico foram empregados os seguintes métodos: PAS, Sudan Black B, detecção da mieloperoxidase, da fosfatase ácida, da fosfatase alcalina e da esterase. Para a demonstração das metaloproteinases MMP 2 e 9 foi empregado o método imunocitoquímico indireto utilizando-se o complexo avidina-biotina-peroxidase. Para a análise ultra-estrutural, após centrifugação do sangue total, o creme leucocitário foi fixado em Karnovsky e processado segundo o método convencional para MET. No sangue do Centropomus parallelus foram encontrados 5 tipos de células: eritrócitos, trombócitos, neutrófilos, linfócitos e monócitos. Os eritrócitos apresentaram formas esférica, oval ou elíptica e o núcleo acompanhando a forma ...
Name:C.I.Solvent Black 5,C.I.50415 Molecular Structure: azine class C.I.Solvent Black 5,C.I.50415,CAS 11099-03-9/52276-97-8/8005-01-4,Nigrosine Spirit Soluble,Basonyl Black X22,Nigrosine Spirit Soluble,Oil Black B,Solvent Black B C.I.Solvent Black 5,C.I.50415,CAS 11099-03-9/52276-97-8/8005-01-4,Nigrosine Spirit Soluble,Basonyl Black X22,Nigrosine Spirit Soluble,Oil Black B,Solvent Black B Molecular Formula: Molecular Weight: CAS Registry Number:11099-03-9/52276-97-8/8005-01-4
هدف این مطالعه جدا کردن اکتینومیست‌های نمک‌دوست و تحمل‌پذیر نمک با توانایی رنگبری از مواد رنگزای آزو است. به این منظور، اکتینومیست‌های نمک‌دوست و تحمل‌پذیر نمک جدا شده از پساب کارخانه نساجی مرینوس قم و دریافت شده از مرکز ذخایر ژنتیکی زیستی ایران، با توجه به توانایی رنگبری از ماده رنگزای آزوی Remazol Black B مورد غربالگری قرار گرفتند و سویه‌های M18 و M77 بیشترین رنگبری را نشان دادند. توانایی رنگبری از ماده رنگزای Remazol Black B توسط این دو سویه در حضور نمک سدیم‌کلراید (0تا 15%)، pH (11-5)، محدود دمایی (C 40-25) در شرایط ایستا مورد بررسی قرار گرفت. بهترین درصد رنگبری برای
DI-fusion, le Dépôt institutionnel numérique de lULB, est loutil de référencementde la production scientifique de lULB.Linterface de recherche DI-fusion permet de consulter les publications des chercheurs de lULB et les thèses qui y ont été défendues.
I have had very good results staining nerve fibers with Kiernans physically developed silver method [Kiernan, HISTOLOGICAL AND HISTOCHEMICAL METHODS, 3rd ed., pp371-373]. Allen A. Smith, Ph.D. Professor of Anatomy School of Graduate Medical Sciences Barry University Miami Shores, FL -----Original Message----- From: [email protected] [mailto:[email protected]] On Behalf Of Chow Wai Lyn, Adeline Sent: Tuesday, January 13, 2004 10:53 PM To: [email protected] Subject: [Histonet] PGP 9.5 Im using PGP 9.5 to stain for nerve fibres in the epidermis of skin taken from the hypothenar region. The results have been inconsistent so far , sometimes staining well, sometimes not. Sections are 30um and stained overnight room temp. using DAKO envision kit. Any advice ? Thanks.. Lyn Research Assistant National University of Singapore _______________________________________________ Histonet mailing list [email protected] ...
The serosa consists of ordinary loose fibrous connective tissue with a delicate covering of mesothelium (simple squamous epithelium derived from mesoderm). The serosa is continuous with the mesentery. ...
noun): An autoimmune disease that affects the blood vessels and connective tissue; fibrous connective tissue is deposited in the skin ...
The purpose of this study is to present an instance of pneumonia brought about by two different oils and to evaluate the simple histochemical method used for th
Conjunctival cytology analysis (stained with periodic acid-Schiff, 40×).Notes: (A) refers to the staining before injection. (B) represents the staining 1 mon
TY - JOUR. T1 - Identification of the Ulex europaeus agglutinin-I-binding protein as a unique glycoform of the neural cell adhesion molecule in the olfactory sensory axons of adult rats. AU - Pestean, Aurelian. AU - Krizbai, István. AU - Böttcher, Herma. AU - Párducz, Árpád. AU - Joó, Ferenc. AU - Wolff, Joachim R.. PY - 1995/8/4. Y1 - 1995/8/4. N2 - Histochemical localization of two lectins, Ulex europaeus agglutinin-I (UEA-I) and Tetragonolobus purpureus (TPA), was studied in the olfactory bulb of adult rats. In contrast to TPA, UEA-I detected a fucosylated glycoprotein that is only present in the surface membranes of olfactory sensory cells including the whole course of their neurites up to the final arborization in glomeruli. Immunoblotting revealed that UEA-I binds specifically to a protein of 205 kDa, while TPA stains several other glycoproteins. Affinity chromatography with the use of a UEA-I column identified the 205 kDa protein as a glycoform of neural cell adhesion molecule ...
PubMed journal article: Morphological and histochemical characteristics of the lamina propria in scrotal and abdominal testes from postpubertal boars: correlation with the appearance of the seminiferous epithelium. Download Prime PubMed App to iPhone, iPad, or Android
Succinate dehydrogenase activity was investigated histochemically in the rat pineal gland. The influence of fixation on the activity pattern, the possible diffusion of enzyme, the nothing...
Minute Peroxidase Suppressor is designed to inhibit endogenous peroxidase activity commonly encountered in immunohistochemistry (IHC) procedures. Inhibiting endogenous peroxidase activity is essential for avoiding false positive and reduce the background of IHC. Many chemical agents such as H2O2 and sodium aside have been used for this purpose but the inhibition is usually incomplete. Minute TM HRP Suppressor is a mixture of several potent HRP inhibitors and the result is a complete inhibitionof endogenous peroxidase activity. The suppressor can be used in regular IHC as well as HRP-mediated super sensitive procedures such as tyramide signal amplification (TSA). Major Features: Very stable at RT, ready to use and irreversible inhibition of endogenous peroxidase activity. Significantly reduce the background of sensitive assays using HRP. 1. After cell or tissue samples are prepared on slide, perform antigen retrieval step if necessary. 2. Block non-specific sites in the samples with normal serum ...
A bone induction model was used to study the effect of different pretreatments and inhibitors added to the substrate of acid phosphatise. Two different acid phosphatises (AcP) were demonstrated. The AcP of matrix-producing cells exhibited resistance to pre-treatment with EDTA while it was sensitive to tartrate added to the incubation medium. The AcP in dentin resorbing cells was EDTA sensitive but resistant to tartrate. Both resorbing and matrix-producing cells were generally sensitive to fluoride, copper and molybdate. In some of the dentionoclasts, fluoride resistant AcP was observed. The results support previous investigations on normal bone formation suggesting that two different isoenzymes of AcP exists. One of these seems to be related to matrix production and mineralization of the new ...
I (co-author R. Elsner) fell under Ken Norris spell in the early 1960s when I began working in Pete Scholanders lab at Scripps Institution of Oceanography. Subsequent interactions with Ken, the Renaissance Scientist, at UCLA and Hawaii did much to shape the course of my interests and research relating to marine mammals, a precious association for which I am forever grateful. ...
Define cytochemistry. cytochemistry synonyms, cytochemistry pronunciation, cytochemistry translation, English dictionary definition of cytochemistry. n. The branch of biochemistry that deals with the study of the chemical composition and activity of cells. cy′to·chem′i·cal adj. n the chemistry of living...
The intervertebral disc is basically made up of two parts and is often compared to a jelly donut. This donut-like structure is porous much like a sponge and (when healthy) is filled with fluid.. The center of this disc contains a jelly-like sack called the nucleus that - along with the fluid in the disc itself - acts like a hydraulic shock absorber.. The outer portion of the donut is called the annulus and is a series of concentric rings of fibrous connective tissue that surrounds the nucleus much like a ring of forts built one inside the other.. The top and bottom of the disc are capped with more of this fibrous connective tissue and these caps are called end plates. It should be noted that the end plates are strongly attached to the vertebrae above and below making it virtually impossible for the disc to slip.. ...