TY - JOUR. T1 - Mechanism of bradykinin-induced histamine release from rat peritoneal mast cells. AU - Zhao, Qiu E.. AU - Mihara, Takuma. AU - Sugimoto, Yukio. AU - Kamei, Chiaki. PY - 1996/2. Y1 - 1996/2. N2 - Bradykinin at concentrations higher than 2 μM caused a significant histamine release from rat peritoneal mast cells when extracellular Ca2+ was removed from the medium. Under the same experimental conditions, bradykinin increased Ca2+ release from the intracellular Ca store of the rat peritoneal mast cells, and a clear relationship was observed between the magnitude of histamine release and an increase in fluorescence intensity. Addition of Ca2+ to the medium resulted in an inhibition of the response to bradykinin in a concentration-dependent manner. Almost the same results were obtained when Mg2+, Ba2+ and La3+ were added to the medium. Neither B1 nor B2 antagonists caused significant antagonistic effects on histamine release induced by bradykinin. However, B2 antagonists caused a ...

TY - JOUR. T1 - Spontaneous histamine release and histamine content in normal subjects and subjects with asthma. AU - Akagi, Katsumi. AU - Townley, Robert G.. PY - 1989/4. Y1 - 1989/4. N2 - Spontaneous histamine release (SHR) from basophils by simple incubation at 37 °C for 60 minutes and histamine content of basophils were assessed in normal subjects, patients with asthma, and methacholine-sensitive subjects without asthma (NAMS). SHR from basophils of normal subjects did not exceed 10% of the total histamine. A significantly higher SHR was observed in basophils from subjects with asthma than from normal subjects (p ,0.002). Basophil SHR in patients with asthma not receiving medication was significantly greater than that in patients with asthma receiving medication (p ,0.05). SHR from basophils in NAMS subjects was similar to that in normal subjects. SHR was highly dependent on temperature and Ca++ and Mg++ ions and appeared to be a slower event than anaphylactic release. The mean histamine ...

The tea made with leaves and stems of plant Anchietia salutaris is traditionally used in Brazil to treat allergies. We examined the effects of a crude aqueous extract and of purified fractions of this plant on the histamine release induced in rat and guinea pig tissues. The crude extract (3-10 mu g/ml) inhibits the histamine release induced by compound 48/80 (0.5 mu g/ml) and antigen in rat peritoneal mast cells. The inhibition is significant after 10 s of preincubation and is completed after 3 min. The crude extract dissolved in the perfusion fluid (1-30 mu g/ml) also inhibits the histamine release induced in guinea pig heart by cardiac anaphylaxis and in hearts from pretreated animals (10-100 mg/kg i.p.). In pretreated animals, the effect manifests after 3 h, is maximum after 12 h and disappears after 48 h. The histamine release induced in isolated guinea pig heart by ionophore A23187 is inhibited by similar doses as in antigen-induced histamine release. Extraction with solvents concentrated ...

Basophils possess membrane bound IgE molecules, and immunological activation leads to a secretory process with cell degranulation and histamine release. Heterologous anti IgE, concanavaline A, and phytohaemagglutinin are potent non-cytotoxic releasing agents. They operate by a mechanism similar to that of immunological activation. Heavy water is not a histamine releasing inducer but it increases histamine release of the cells. We studied the histamine release reaction of leukaemic basophils in 10 patients and found a physiological response such as that previously reported with normal human basophils.. ...

Journal of Immunology Research is a peer-reviewed, Open Access journal that provides a platform for scientists and clinicians working in different areas of immunology and therapy. The journal publishes research articles, review articles, as well as clinical studies related to classical immunology, molecular immunology, clinical immunology, cancer immunology, transplantation immunology, immune pathology, immunodeficiency, autoimmune diseases, immune disorders, and immunotherapy.

Most histamine in the body is generated in granules in mast cells and in white blood cells called basophils andeosinophils. Mast cells are especially numerous at sites of potential injury -- the nose, mouth, and feet, internal body surfaces, and blood vessels. Non-mast cell histamine is found in several tissues, including the brain, where it functions as a neurotransmitter. Another important site of histamine storage and release is the enterochromaffin-like (ECL) cell of the stomach.. The most important pathophysiologic mechanism of mast cell and basophil histamine release is immunologic. These cells, if sensitized byIgE antibodies attached to their membranes, degranulate when exposed to the appropriate antigen. Certain amines and alkaloids, including such drugs as morphine, and curare alkaloids, can displace histamine in granules and cause its release. Antibiotics likepolymyxin are also found to stimulate histamine release.. Histamine release occurs when allergens bind to mast-cell-bound IgE ...

The aim of this study was to evaluate whether Na+ exerts its inhibitory effect on basophil histamine release induced by immunoglobulin E (IgE)-dependent (anti-IgE) and IgE-independent (N-formyl-methionyl-leucyl-phenylalanine (FMLP), interleukin-3 (IL-3)) stimuli in patients with allergic rhinitis (n = 24) and allergic bronchial asthma (n = 10). Peripheral blood leucocytes were stimulated with anti-IgE, FMLP and IL-3 in the presence of high and low Na+ concentrations, and histamine release was measured using a fluorometric method. In standard Na(+)-containing medium, spontaneous and stimulated histamine release was higher in allergic patients (n = 34) (both rhinitic and asthmatic) than in healthy subjects (n = 41). Na+ removal from extracellular medium and its isosmotic substitution with choline chloride or with N-methyl-D-glucamine led to a significant increase of anti-IgE-, FMLP- and IL-3-induced histamine release in normal subjects, but not in allergic patients. The increase in Na+ ...

The actions of adenosine on histamine release of human lung fragments were investigated. Histamine release was stimulated either with the calcium ionophore A 23187 orwith concanavalin A. Adenosine and its analogue 5-N-ethylcarboxamidoadenosine alone had no significant effect on basal release or on the release elicited by A 23187 or concanavalin A. However, in the presence of the adenosine receptor antagonist 8-[4-[[[[(2-aminoethyl)amino]-carbonyl] methyloxy]-phenyl]-1,3-dipropylaxanthine (XAC), which itself did not affect the release, adenosine increased the stimulated histamine release. On the other hand, in the presence of the nucleoside transport inhibitor S-(p-nitrobenzyl)-6-thioninosine (NBTI), adenosine caused a reduction in stimulated histamine release. NBTI itself caused a stimulation of release. Thus, a stimulatory effect of adenosine was seen in the presence ofXAC, whereas an inhibitory effect was unmasked by NBTI. From these data it is concluded that adenosine exerts two opposing ...

The actions of adenosine on histamine release of human lung fragments were investigated. Histamine release was stimulated either with the calcium ionophore A 23187 orwith concanavalin A. Adenosine and its analogue 5-N-ethylcarboxamidoadenosine alone had no significant effect on basal release or on the release elicited by A 23187 or concanavalin A. However, in the presence of the adenosine receptor antagonist 8-[4-[[[[(2-aminoethyl)amino]-carbonyl] methyloxy]-phenyl]-1,3-dipropylaxanthine (XAC), which itself did not affect the release, adenosine increased the stimulated histamine release. On the other hand, in the presence of the nucleoside transport inhibitor S-(p-nitrobenzyl)-6-thioninosine (NBTI), adenosine caused a reduction in stimulated histamine release. NBTI itself caused a stimulation of release. Thus, a stimulatory effect of adenosine was seen in the presence ofXAC, whereas an inhibitory effect was unmasked by NBTI. From these data it is concluded that adenosine exerts two opposing ...

in Naunyn-Schmiedebergs Archives of Pharmacology (1998), 357(4), 357-62. Methoctramine, a selective M2 muscarinic cholinergic receptor antagonist, has been reported to activate phosphoinositide breakdown at high concentrations. Its polyamine structure suggests a putative ... [more ▼]. Methoctramine, a selective M2 muscarinic cholinergic receptor antagonist, has been reported to activate phosphoinositide breakdown at high concentrations. Its polyamine structure suggests a putative activation of guanine nucleotide-binding proteins (G proteins). Incubation of methoctramine with rat peritoneal mast cells resulted in a dose-dependent noncytotoxic histamine release, with an EC50 of 20 microM and a maximum effect at 1 mM. Atropine, pirenzepine and HHSiD neither inhibited methoctramine-induced histamine release nor stimulated histamine release. Histamine release and inositol phosphates generation induced by methoctramine were both inhibited by pertussis toxin pretreatment. Benzalkonium chloride, a ...

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Mita, H., Yasueda, H. and Akiyama, K. (2000), Affinity of IgE antibody to antigen influences allergen-induced histamine release. Clinical & Experimental Allergy, 30: 1583-1589. doi: 10.1046/j.1365-2222.2000.00921.x ...

Tree pollens, grasses, ragweed, and outdoor molds are common allergens that can enter the body through mucus membranes found in the nose and respiratory tract. In response, some people may overproduce an antibody known as immunoglobulin E (IgE), leading the immune system to overreact. IgE travels to specific immune cells called mast cells which trigger the release of mediators like histamines to help get rid of these allergens. Histamine release initiates the very familiar allergy symptoms of sneezing, itchy and watery eyes, nasal congestion, and runny noses.. Unfortunately, with the increase of allergic conditions over the last 70 years in high-income countries, their occurrence is now a worldwide public concern. Genetic changes, environmental exposure, and nutritional changes in food may all be contributing factors to this rising trend. Thankfully there are complementary solutions to help manage symptoms, support immune function, and stabilize mast cells to reduce histamine release, so we can ...

The Translational Controlled Tumour Protein TCTP (gene symbol TPT1, also called P21, P23, Q23, fortilin or histamine-releasing factor, HRF) is a highly conserved protein present in essentially all eukaryotic organisms and involved in many fundamental cell biological and disease processes. It was first discovered about 35 years ago, and it took an extended period of time for its multiple functions to be revealed, and even today we do not yet fully understand all the details. Having witnessed most of this history, in this chapter, I give a brief overview and review the current knowledge on the structure, biological functions, disease involvements and cellular regulation of this protein ...

Of clinical interest is also the quantification of the histamine release from basophilic leucocytes in allergies (please refer to the add-on kit Histamine Release; reference no. BA E-1100).. ...

How Delivering Free Radical Therapy According to the Designed2Win Health Model Provided a Positive Outcome. Every now and then a FRT health provider may run into a client who presents a confusing situation that hadnt been solved with meds, and really cant get solved through FRT without returning to its health model basis. An example is a recent client who demonstrated an eosinophilic white blood count of more than 20%. Less than 4% is normal, so 20% is not healthy, especially when allowed to remain that high, as the amount of toxins released by eosinophils aimed at killing parasites but which can be stimulated by any allergen are certain to damage healthy tissues. Such a scenario affecting the lungs can cause severe compromise in lung function, leading to COPD, asthma or a tumor.. In this scenario the culprit is generally some allergen that has stimulated both the mast cells and the basophilic white blood cells to release histamine. The histamine helps stimulate bone marrow to produce more ...

There are seven types of white blood cells, two of which are primarily involved in the inflammation process. Eosinophils control allergic responses, while basophils release histamine during an inflammatory response ...

C3a is an anaphylatoxin that triggers a response by stimulating inflammatory cells to release histamine, enzymes, cytokines and other mediators.

How MRT Compares to IgG: There are a number of advantages of MRT over any form of IgG testing. MRT is an endpoint test, meaning that all the immune based adverse reactions that end up causing a mediator release in the blood will be identified. So MRT does this without caring about the mechanism (such as IgG). In fact MRT is able to take into account the actions of all mechanisms, whether they are antibodies or other, because all of them ultimately cause white blood cells to release mediators. MRT is able to account for a much wider array of reactions than the relatively simple IgG testing. In addition, MRT is able to identify reactions to chemicals. Overall MRT is more accurate an useful clinically.. The ALCAT Test: The ALCAT test was invented and patented by the same person who invented and patented MRT, Dr Mark Pasula. The two technologies are similar, yet separately patented, which means there is a unique difference. The main difference between the two tests is in terms of accuracy and ...

Release Notes for Cisco AnyConnect Secure Mobility Client, Release 4.9 -Release Notes: Release Notes for Cisco AnyConnect Secure Mobility Client, Release 4.9

If individual updates, such as maintenance releases or diagnostic updates, were installed after a feature release, you must select this option to uninstall the individual updates before the new feature release is installed. If the option is not selected, errors might occur during the feature release installation. ...

The KDE Community is happy to announce the immediate availability of the second release candidate for KDE 4.0. This release candidate marks the last mile on the road to KDE 4.0. This release sees increasing participation from distributions, you can download packages for Debian, Kubuntu, Mandriva, openSUSE & Fedora or grab the live CDs from Kubuntu & openSUSE.

The KDE Community is happy to announce the immediate availability of the second release candidate for KDE 4.0. This release candidate marks the last mile on the road to KDE 4.0. This release sees increasing participation from distributions, you can download packages for Debian, Kubuntu, Mandriva, openSUSE & Fedora or grab the live CDs from Kubuntu & openSUSE. KDEs

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Added highlighting the account status bar, if the account is in Margin Call or Stop Out states. Fixed drawing of the Fibonacci Fan... - Release Notes

I've redone libmii to add support for all aspects of your miis. In addition to now being able to view all info about your mii in text form, I've also whipped up some code to graphically represent your miis. I'm going to release the new libmii along with the sprites and GRRLIB code fo

I've redone libmii to add support for all aspects of your miis. In addition to now being able to view all info about your mii in text form, I've also whipped up some code to graphically represent your miis. I'm going to release the new libmii along with the sprites and GRRLIB code fo

I am pleased to announce the release of the OWASP ModSecurity Core Rule Set (CRS) v2.1.0. This is a significant update as we have added many new capabilities. CHANGE LOG - -------------------------- Version 2.1.0 - 12/29/2010 -------------------------- Improvements: - Added...

TY - JOUR. T1 - Effect of loratadine on histamine release induced by antigen-antibody reaction. AU - Kamei, Chiaki. AU - Sugimoto, Yukio. AU - Yamaji, Masako. AU - Takada, Miho. PY - 1996. Y1 - 1996. N2 - Loratadine caused an inhibition of histamine release from rat peritoneal mast culls induced by passively sensitized mast cells, and IC 50 was 9.57 μM. SCH 34117, a metabolite of loratadine, also inhibited histamine release from mast cells, and its potency was more than that of loratadine. Moreover, in ex vivo experiments, loratadine (5 mg/kg, p.o.) as well as terfenadine provided a relatively potent inhibitory effect on histamine release from lung pieces of actively sensitized guinea pigs exposed to antigen.. AB - Loratadine caused an inhibition of histamine release from rat peritoneal mast culls induced by passively sensitized mast cells, and IC 50 was 9.57 μM. SCH 34117, a metabolite of loratadine, also inhibited histamine release from mast cells, and its potency was more than that of ...

In order to clarify the pathogenetic role of basophils and mast cells in chronic urticaria, histamine and leukotriene (LT)C4 release was examined in washed mixed leukocytes (n = 8) and skin mast cells (n = 5) from patients with chronic urticaria and compared with the same cells from normal controls (n = 9). Anti-IgE-stimulated basophil histamine release was significantly reduced in urticaria patients (median 2.9% vs 15.1% in normal controls), whereas histamine release to A23187, FMLP, and PAF, as well as anti-IgE-induced LTC4 release, showed no differences in both groups. In contrast, anti-IgE-stimulated skin mast cells from urticaria patients reacted similarly to those of controls (median histamine release 11.4% vs 14.2% in normal controls). Pretreatment of the cells with interleukin (IL)-3 upregulated responsiveness of basophil histamine release to anti-IgE in urticaria patients (median histamine release 14.3%), but pretreatment with the H2-antagonist cimetidine showed no effect. These data ...

Latexin, a protein possessing inhibitory activity against rat carboxypeptidase A1 (CPA1) and CPA2, is expressed in a neuronal subset in the cerebral cortex and cells in other neural and non-neural tissues of rat. Although latexin also inhibits mast-cell CPA (MCCPA), the expression of latexin in rat mast cells has not previously been confirmed. In the present study we examined the expression and subcellular localization of latexin in rat peritoneal mast cells. Western blot and reverse-transcriptase-mediated PCR analyses showed that latexin was contained and expressed in the rat peritoneal mast cells. Immunocytochemically, latexin immunofluorescence was localized on granular structures distinct from MCCPA-, histamine- or cathepsin D-immunopositive granules. Immunoelectron microscopy revealed that latexin was associated with a minority population of granules. The latexin-associated granules were separated from MCCPA- or histamine-containing granules on a self-generating density gradient of ...

TY - JOUR. T1 - Diamine oxidase-gold ultrastructural localization of histamine in isolated human lung mast cells stimulated to undergo anaphylactic degranulation and recovery in vitro. AU - Dvorak, Ann M.. AU - Morgan, Ellen S.. AU - Schleimer, Robert P.. AU - Lichtenstein, Lawrence M.. PY - 1996/1/1. Y1 - 1996/1/1. N2 - A new enzyme-affinity-gold ultrastructural method makes use of the affinity of the enzyme, diamine oxidase coupled to gold, for its substrate, histamine, for localization of histamine in isolated human lung mast cells (HLMCs). The method works with routinely prepared ultrastructural samples, thereby allowing precise identification of ultrastructural structures that contain histamine. We used this method to identify the release of histamine from granule stores in anti-immunoglobulin-E (IgE)-stimulated HLMCs and the replacement of histamine in secretory granules of HLMCs during recovery from anaphylactic degranulation in vitro. The findings show that electron-dense granules in ...

TY - JOUR. T1 - Inhibitory effect of nifedipine and cromolyn sodium on skin reactions and 45ca uptake and histamine release in rat mast cells induced by various stimulating agents. AU - Tanizaki, Yoshiro. AU - Akagi, Katsumi. AU - Lee, Kyung Nam. AU - Townley, Robert G.. PY - 1983. Y1 - 1983. N2 - The effect of nifedipine, one of the calcium channel antagonists, was examined in allergic and nonspecific reactions using rat skin and peritoneal mast cells. Rat passive cutaneous anaphylaxis response mediated by IgE antibody was inhibited by nifedipine (100 μg/rat) and also by cromolyn sodium (5 mg/rat). Skin reactions induced by histamine or methacholine were not inhibited, and skin reaction induced by serotonin was slightly inhibited by these drugs. The 45Ca uptake and histamine release in sensitized mast cells stimulated by ovalbumin were suppressed by nifedipine. In actively sensitized cells, nifedipine seems to be more effective on the inhibition of 45Ca uptake, but less effective on histamine ...

The mechanism of chronic mast cell activation in asthma is unclear. Monomeric immunoglobulin (Ig)E in the absence of allergen induces mediator release from rodent mast cells, indicating a possible role for IgE in the continued activation of mast cells within the asthmatic bronchial mucosa. In this study it was investigated whether monomeric IgE induces Ca2+ influx and mediator release from human lung mast cells (HLMC). Purified HLMC were cultured for 4 weeks and then exposed to monomeric human myeloma IgE. Ratiometric Ca2+ imaging was performed on single fura-2-loaded cells. Histamine release was measured by radioenzymatic assay; leukotriene C4 (LTC4) and interleukin (IL)-8 were measured by ELISA. At concentrations experienced in vivo, monomeric IgE induced dose-dependent histamine release, LTC4 production and IL-8 synthesis. This was associated with a rise in cytosolic free Ca2+. Enhanced histamine release was still evident 1 week after initial exposure to IgE suggesting that continued exposure ...

Human lung mast cells (HLMCs) play a central role in asthma pathogenesis through their relocation to the airway smooth muscle (ASM) bundles. β2 adrenoceptor (β2-AR)-agonists are used to relieve bronchoconstriction in asthma, but may reduce asthma control, particularly when used as monotherapy. We hypothesized that HLMC and human ASM cell (HASMC) responsiveness to β2-AR agonists would be attenuated when HLMCs are in contact with HASMCs. Cells were cultured in the presence of the short-acting β2-agonist albuterol, and the long-acting β2-agonists formoterol and olodaterol. Constitutive and FcεRI-dependent HLMC histamine release, HASMC contraction, and β2-AR phosphorylation at Tyr350 were assessed. Constitutive HLMC histamine release was increased in HLMC-HASMC coculture and this was enhanced by β2-AR agonists. Inhibition of FcεRI-dependent HLMC mediator release by β2-agonists was greatly reduced in HLMC-HASMC coculture. These effects were reversed by neutralization of stem cell factor ...

Cromone therapy is thought to inhibit IgE-dependent histamine release from mast cells, although the effects of sodium cromoglycate on human lung mast cells are weak. Both agents have weak effects on eosinophils and on macrophage activity but do not affect airway smooth muscle tone.

We investigated the ability of antigen-IgE interactions to stimulate histamine release from human infant cutaneous mast cells. Skin obtained at circumcision contained numerous perivascular mast cells, as assessed by light and electron microscopy. The histamine content of this tissue averaged 17.7 ng (+/- 1.5 SEM)/mg wet weight. Challenge of 200-microns thick sections of unsensitized skin with varying concentrations of monoclonal murine antibodies to human IgE caused no net release of histamine. After skin sections were incubated in the presence of 5 micrograms/ml of human myeloma IgE (S) for 120 min at 37 degrees C, monoclonal anti-IgE challenge resulted in 40.1% (+/- 6.0 SEM) histamine release. Similar passive sensitization with 1/20 dilutions of serum from humans expressing IgE to purified Juniperus sabinoides (JS) antigen rendered the tissue responsive to specific antigen challenge. Dose-related histamine release occurred over 30 min with optimal release of 12.6% (+/- 2.4 SEM) after ...

TY - JOUR. T1 - Comparative effect of recombinant IL-1, -2, -3, -4, and -6, IFN-γ, granulocyte-macrophage-colony-stimulating factor, tumor necrosis factor-α, and histamine-releasing factors on the secretion of histamine from basophils. AU - Alam, R.. AU - Welter, J. B.. AU - Forsythe, P. A.. AU - Lett-Brown, M. A.. AU - Grant, J. A.. PY - 1989. Y1 - 1989. N2 - Most cytokines possess multiple biologic activities. This study was undertaken to investigate the effect of rIL-1β, -2, -3, -4 and -6, IFN-γ, TNF-α, and granulocyte-macrophage (GM)-CSF on basophils from 16 donors and the amount of histamine releases was compared with that by partially purified mononuclear cell-derived histamine-releasing factor (HRF) and anti-IgE. We found that only IL-3 and GM-CSF at relatively high doses (50 to 500 ng/ml) released small amounts of histamine (3 to 14%) from two allergic donors. In contrast, both HRF and anti-IgE released significant amounts of histamine from all donors. Other cytokines did not ...

TY - JOUR. T1 - Cyclosporin A rapidly inhibits mediator release from human basophils presumably by interacting with cyclophilin. AU - Cirillo, Raffaele. AU - Triggiani, Massimo. AU - Siri, Lorenzo. AU - Ciccarelli, Anna. AU - Pettit, George. AU - Condorelli, Mario. AU - Marone, Gianni. PY - 1990. Y1 - 1990. N2 - We have examined the effects of cyclosporin A (CsA) and a series of CsA analogs that bind with decreasing affinity to cyclophilin, to evaluate the involvement of this protein in the release of preformed (histamine) and de novo synthesized (peptide leukotriene C4; LTC4) mediators of inflammatory reactions from human basophils. CsA (8 to 800 nM) concentration-dependently inhibited (5 to 60%) histamine release from peripheral blood basophils challenged with anti-IgE. CsA was more potent (92.6 ± 1.8 vs 59.1 ± 4.5%; p , 0.001) and, at low concentrations, more effective when the channel-operated influx of Ca2+ as bypassed by the ionophore A23187 (IC40 = 24.1 ± 3.9 vs 105.5 ± 22.2 nM; p , ...

The release mechanism of chemical mediators from basophils and mast cells was discussed when these cells were stimulated by different antigens and anti-IgE. 1. Ca(2+) influx into mast cells increased after stimulation by antigen. The increased Ca(2+) uptake by mast cells was inhibited by antiallergic agents, disodium cromoglycate (DSCG) and tranilast, and calcium antagonists, nifedipine and nicardipine. 2. The dose-response curve of histamine release by antigen was different from that by anti-IgE. The maximum release of histamine by house dust was, however, similar to that by anti-IgE. House dust- and anti-IgE-induced release of histamine increased with higher- serum IgE levels. On the contrary, the release of histamine by Candida albicans was not releated to serum IgE levels, and the release by C. albicans did not parallel therelease by anti-IgE ...

Basophils are a rare granulocyte population that has been associated with allergic and inflammatory responses. It is essential to understand the regulatory mechanisms by which basophils are kept in check, considering the impact of dysregulated basophil function on immune responses under different pathological conditions. Among immunoregulatory cells, CD4+CD25+FoxP3+ regulatory T cells (Tregs) are the key players that maintain immune tolerance. The mechanisms by which Tregs regulate and suppress diverse immune cell subsets have been studied extensively, but the impact of Tregs on basophil functions is not well understood. We report that human basophils are refractory to Treg-mediated suppression and found that Tregs stimulate resting basophils to induce the expression of activation markers including CD69, CD203c, and CD13 and the release of basophil cytokines including IL-13, IL-8, and IL-4. Mechanistically, Tregs could induce human basophil activation via IL-3 and STAT5 activation, whereas ...

The nonselective beta-adrenoceptor agonist, isoprenaline (pD2; 8.8 +/- 0.2), and selective beta2-adrenoceptor agonists, clenbuterol (9.2 +/- 0.4) and salbutamol (7.1 +/- 0.1), inhibited the immunoglobulin E-mediated release of histamine from human lung mast cells in a concentration-dependent manner. …

Various allergic diseases cause allergic inflammation, which is mediated by mast cells. The current study investigated the anti‑allergic inflammatory effects of formononetin and its mechanism of action in vitro using mast cells. Levels of histamine and pro‑inflammatory cytokines, including tumor necrosis factor‑α (TNF‑α), interleukin (IL)‑1β and IL‑6, were measured to assess the effects of formononetin on allergic inflammation. The activation of intracellular calcium and nuclear factor (NF)‑κB, as well as the activity of caspase‑1, were assessed to determine the mechanism of action. It was determined that difference concentrations of formononetin (0.1, 1 and 10 µM) suppressed histamine release and secretion of TNF‑α, IL‑1β and IL‑6. Further investigations indicated that the effects of formononetin were associated with a reduction of intracellular calcium, suppression of NF‑κB activation and upstream IκKα phosphorylation and inhibition of caspase‑1 activity. ...

TY - JOUR. T1 - Histamine release from human leukocytes by human recombinant interleukin-3. AU - Kanamori, S.. AU - Urisu, A.. AU - Iimi, K.. AU - Kondo, Y.. AU - Horiba, F.. AU - Masuda, S.. AU - Tsuruta, M.. AU - Yazaki, T.. N1 - Copyright: Copyright 2004 Elsevier B.V., All rights reserved.. PY - 1990. Y1 - 1990. UR - http://www.scopus.com/inward/record.url?scp=0025120802&partnerID=8YFLogxK. UR - http://www.scopus.com/inward/citedby.url?scp=0025120802&partnerID=8YFLogxK. M3 - Article. C2 - 1697456. AN - SCOPUS:0025120802. VL - 39. SP - 452. EP - 458. JO - Japanese Journal of Allergology. JF - Japanese Journal of Allergology. SN - 0021-4884. IS - 5. ER - ...

TY - JOUR. T1 - Piceatannol is an effective inhibitor of IgE-mediated secretion from human basophils but is neither selective for this receptor nor acts on syk kinase at concentrations where mediator release inhibition occurs. AU - Miura, K.. AU - Lavens-Phillips, S.. AU - MacGlashan, D. W.. PY - 2001/12/1. Y1 - 2001/12/1. N2 - Background: Syk kinase is probably an early necessary tyrosine kinase involved in IgE-mediated secretion from human basophils. Causal testing of the role ofsyk kinase in the secretion requires a selective pharmacological agent. Piceatannol has previously been used to demonstrate the causal role of syk in secretion but its selectively has recently come into question. Objective: To determine whether piceatannol inhibits IgE-mediated signalling events in a manner consistent with its putative inhibitory effects on syk kinase and at concentrations relevant to its inhibition of mediator release. Methods: Human basophils were examined for the effects of piceatannol on mediator ...

Four subjects with clinical histories of milk-induced asthma were studied (three allergic to cows milk; one to soya milk). In each instance, skin prick tests, RAST (IgE and IgG4), the basophil histamine release, and serum precipitins, using appropri

Hello arek, You don?t tell why you have a high histamine. Histamine levels can become elevated for many reasons,and are usually temporary. Anything recommended for lowering your level must be run by your physician. Drug interactions can be problematic and even fatal in persons with an elevated histamine, so please check before attempting to treat yourself. Allergies, surgical procedures, parasitic infections (real parasitic infections such as those contracted from eating raw fish), certain fish and shellfish, fermented foods, wines, salamis, etc. can cause an elevated histamine. http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&list_uids=3530640&dopt=Citation ?In humans, histamine ( -imidazole ethylamine) is the most important mediator and is mostly found in the initial phase of an anaphylactic reaction (immediate type allergy). Histamine is produced by the enzymatic decarboxylation of histidine. In the organism, histamine is present in nearly all tissues, and it is mainly ...

Niacin is important for the manufacture of the sex and adrenal hormones; however, along with its primary prosexual effects, shortly after ingestion, niacin can produce sensations of intense warmth accompanied by a tingly itch throughout the body lasting up to 20 minutes. It may also produce a reddish cast to the skin, which is especially pronounced in the extremities. This response is called the Niacin Flush.. This red, itching warmth caused by niacin use is well known and is harmless. The flush is healthy and indicates that niacin is helping improve blood flow and circulation. Reaction from the Niacin Flush actually mimics an individuals natural sexual response. This natural sex flush, which has been documented by Masters and Johnson, is caused by a large histamine release; in fact, a niacin flush is caused by the same histamine release as the natural sex flush, with one important difference: Because niacin instigates a larger-than-normal histamine release, the results of using niacin are ...

The developers and the researchers have a great responsibility when introducing a new drug into clinical practice, particularly in neuromuscular blocking drugs.7 Dr. Caldwell addresses this issue in his editorial when he compares the side effects of rapacuronium and GW280430A.8 Because we were involved in the clinical evaluation of rapacuronium,9-11 we would like to comment on some relevant aspects of the side effect profiles of both drugs and on the drug approval processes. First, in clinically relevant concentrations, rapacuronium potentates bronchoconstriction most probably by destabilization of the balance between M2 and M3 muscarinic receptors.12 In contrast, GW280430A seems to release histamine3 and therefore may possibly induce bronchoconstriction. Second, although many antihistaminic drugs and prophylactic strategies are available, an effective treatment to rebalance the muscarinic effects of rapacuronium was and is still missing. Third, because rapacuronium did not release histamine,13 ...

Adenosine potentiated anaphylactic histamine release from isolated rat mast cells in a dose-dependent manner between 10−8 and 10−5 M. Adenosine was found to be present during a normal incubation of...

Description: Histamine is naturally occurring in food, with high concentrations associated with spoiled and fermented foods. Exposure to high levels of histamine through the ingestion of food can cause symptoms similar to an allergic response. Our Histamine Assay Kit detects total histamine from food samples using a colorimetric probe. Reduction of the probe yields color development proportional the histamine levels in the sample. Absorbance at 450nm is read after a one hour incubation at 37C and histamine levels are calculated based on a histamine standard curve ...

Methods: Female Balb/c mice were sensitized with 200 μl 0.5% DNCB for three days. After sensitization, mice were challenged with 200 μl 1% DNCB on the same dorsal skin and also 20 μl 1% DNCB on each ear every 3 days, and orally administrated by gavage with DCE (0.6, 1.2 and 2.4 g/kg) daily from day 14 to day 29 for 16 consecutive days. At the end of experiment, the clinical scores for AD on the mice were calculated to evaluate the therapeutic effect of DCE; and serum, ears and dorsal skin of the mice were collected for mechanistic study. The anti-allergic activity of DCE was also evaluated using antigen-induced RBL-2H3 cell line. The release of selected cytokines, chemokines and β-hexosaminidase was measured to determine the anti-allergic activity of DCE. In addition, intracellular Ca2+ level, MAPKs and Lyn phosphorylations were further investigated to reveal its anti-allergic molecular mechanisms ...

Why the immune system mounts an attack against seemingly harmless agents baffles researchers. Because the immune system treats the harmless agent as a threat, B lymphocytes produce immunoglobulin E antibody (IgE). IgE attaches to mast cells such as those present in the nasal and bronchial passages, resulting in histamine release. Inflammation and allergy symptoms follow. Once sensitized, mast cells automatically release histamine when the invading agent reappears. Because IgE can sensitize cells anywhere in the body, individuals symptoms vary (eg, a cat may produce hives in one person and generate sneezing in another person).9,11 Allergy should not be confused with atopy. Atopy indicates that a person has developed a symptomless sensitization. The Increase in Allergies A genetic susceptibility to allergens has been established, but a shift in the human gene pool is an unlikely explanation for the increased prevalence of allergies. Shifts in population-based traits require several generations to ...

If you have MCAS, there are a number of ways to treat the problem. If you want to minimize your symptoms, a low-histamine diet can greatly alleviate the severity of symptoms related to histamine release. You may also want to investigate your sensitivity to salicylate and oxalate as they can trigger the release of histamine secretion.. As mentioned earlier, a digestive imbalance is the key to MCAS. So if you want to treat the problem, you have to make sure you are eating the right foods to treat the imbalance. Here are 5 nutrients that can greatly reset your body and treat MCAS:. 1. Ensure you are taking in your daily recommended dosage of vitamin C in ester form or buffered form. Taking between a daily dosage of 3,000 to 6,000 milligrams can work exceptionally well as a wonderful anti-histamine. It works by reducing mast cells from releasing histamine and causing histamine to break down at a quicker speed.. 2. In order for vitamin C to function at its optimal level, you should also make sure ...

Small, lightweight and battery-powered, Therapik® has been clinically proven to soothe stings and bites from organisms that deliver thermolabile venom and from mosquito saliva containing protein. These proteins cause your skin to release histamines. The type of protein in venom, produced by 20,000 species of insects and sea creatures, can be neutralized with the application of heat by denaturing it. This then stops the skins histamine release that triggered the itch and welts. Using patented technology based on this principle, Therapik® delivers highly concentrated heat to the skin through a small cylindrical opening ...

Allergic subjects release significantly more histamine than normals, exposure to the relevant antigens (P23/TPT1: [§§] locus 13q12-q14) nonessential co-chaperones produces a cytokine (histamine-releasing factor) from a subpopulation of highly allergic donor basophils HRF/p23 can stimulate nonimmune epithelium late allergic reaction (LAR), causing them (basophils) to release histimines. Molecular chaperone machines: Hsp70/Hsp90 and most p53 mutations are associated with a compensatory mutant pair the cyclophilin Cyp-40 and several other polypeptides such as P23 between both partners is through Rx (Resistance to potato virus X) immune sensor stabilization in response to wild type GR glucocorticoid receptor pharmacological properties hormonal stimuli heterocomplexes GR with plant hsp90 are stabilized, principally CyP40, arrives late in receptor complex assembly. GR regulation requires its Hsp90 co-chaperone (PTGES3) function, but not its chaperone activity. A polyclonal rabbit antibody inhibited ...

Purpose of Review: We review basophil testing by flow cytometry with an emphasis on advantages and disadvantages. Recent Findings: There are many tools available to assess the presence and severity of allergic diseases in patients. For 50 years, peripheral blood basophils have been used as tools to study these diseases. It is a very accessible cell that binds IgE antibody and secretes the classical mediators responsible for the symptoms of allergic reactions. In the last decade, an even more accessible methodology, using flow cytometry, has been developed to enhance the ability to use basophils for both mechanistic and clinical diagnostics. Basophil testing has been included in diagnostics for different forms of allergies as well as to monitor disease status. Summary: A variety of studies have begun to establish both precise methods and their clinical relevance for disease diagnosis, but there remain some important questions on how to take optimal advantage of the behaviours of basophils. ...

Mast cell activation disorders involve an unusual accumulation of mast cells that release histamine and other mediators for inappropriate or unknown reasons and cause inflammation, reports the...

How Delivering Free Radical Therapy According to the Designed2Win Health Model Provided a Positive Outcome. Every now and then a FRT health provider may run into a client who presents a confusing situation that hadnt been solved with meds, and really cant get solved through FRT without returning to its health model basis. An example is a recent client who demonstrated an eosinophilic white blood count of more than 20%. Less than 4% is normal, so 20% is not healthy, especially when allowed to remain that high, as the amount of toxins released by eosinophils aimed at killing parasites but which can be stimulated by any allergen are certain to damage healthy tissues. Such a scenario affecting the lungs can cause severe compromise in lung function, leading to COPD, asthma or a tumor.. In this scenario the culprit is generally some allergen that has stimulated both the mast cells and the basophilic white blood cells to release histamine. The histamine helps stimulate bone marrow to produce more ...

Basophil Involved in allergic and inflammatory responses. Basophils release histamine like mast cells, but unlike mast cells they circulate in the blood. Credit: Bret Syfert/Big Picture CC BY BIGPICTUREEDUCATION.COM

Sweet Itch in horses is a skin condition caused by an immune response to the saliva of the insect the biting midge, which causes a histamine release. Although not all horses experience this histamine release, in those that do it leads to extreme swelling. - Wag! (formerly Vetary)

Fingerprint Dive into the research topics of Loss of syk kinase during IgE-mediated stimulation of human basophils. Together they form a unique fingerprint. ...

You can blame your streaming nose on your bodys natural histamine response. When the dedicated patrol members of your immune system, immunoglobulins, detect an invader they rapidly flag other cells in your immune system to take action and release histamine. This is another alarm system that sets off even more body reactions. The cells lining your nose, mouth, even your eyelids are directed to produce and release watery mucus in what seems like gallon quantities ...

Gibbs, Bernhard F and Rathling, A. and Hube, M. and Haas, Helmut (2007) Histamine in allergy, infl ammation, must cells and basophils: Substantial differences in the kinetics of histamine release from human basophils caused by varying strengths of IgE-dependent activators. Inflammation Research, 56 (Supplement 1). S05-S06 . ISSN 1023-3830 . (doi:https://doi.org/10.1007/s00011-006-0502-1 ) (The full text of this publication is not currently available from this repository. You may be able to access a copy if URLs are provided ...

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Prominent for its nutritious value milk can trigger be allergic to few among us, check the list of foods to avoid with dairy allergy.. Dairy allergy triggers when milk or other dairy related products are treated harmful by immune system. To detect proteins in milk products, your immune system produces antibodies, which signals back to release histamines.. Histamines react with protein substances to result adverse allergic symptoms. According to American Academy, Asthma and Immunology, 80 percent of children with this allergy will outgrow when they reach the age group of 16. Approximately 2.5 percent of infants below the age of 3 are hypersensitive to milk.. ...

Seasonal allergies are a real struggle for many people. Allergies start early in the Spring with tree and bloom pollens, closely followed by Summer grasses and then in the Fall with Molds and Dust. A good portion of my patients take an antihistamine daily all year just to function comfortably. Fortunately, there are many many options for support. Allergies occur when the immune system gets diverted down a pathway that starts to see normal environmental proteins (pollens and dust or food proteins) as foreign invaders instead of inert substances. The immune system goes down a very specific pathway called the TH2 pathway. In this pathway, exposure to the allergen causes activation of MAST cells, which release histamine and the activation of Eosinophils, which are the white blood cells associated with the allergic response. It is believed that this pathway evolved for the purposes of protecting us against parasites but since we dont spend as much time living off the land as we previously did, it ...

Allergies can be topical, respiratory, or digestive, and result in an immune system overreaction to a substance. This can release histamines, which cause wheezing, rashes, sneezing, vomiting, and even anaphylactic shock. People can be allergic to specific foods, plants, animals, and chemicals. Human studies are lacking, and more research is necessary on the anti-allergy potential of various herbs.. Hay fever is a seasonal allergy to pollens and fungi in the air, and can irritate the nose, throat, and eyes. ...

In people who are not allergic, the mucus in the nasal passages simply moves foreign particles to the throat, where they are swallowed or coughed out. But something different happens to a person who is sensitive to airborne allergens.. As soon as the allergen lands on the mucous membranes lining the inside of the nose, a chain reaction occurs that leads the mast cells in these tissues to release histamine and other chemicals. These powerful chemicals contact certain cells that line some small blood vessels in the nose. This allows fluids to escape, which causes the nasal passages to swell, resulting in nasal congestion.. Created by National Institute of Allergy and Infectious Diseases ...

Allergic reactions are the primary cause of hives. It can be difficult to determine the specific inciting factors, but there are numerous stimuli that can cause them to appear. Food products, plant contact or ingested medications can cause hives. Physiologically, they occur when there is a large histamine release in the body from specialized blood cells called mast cells. The histamines cause fluid to leak around the blood vessels in the area they appear leading to the skin changes that can be easily seen.. ...

INDICATIONS. Haridra has many medicinal properties - it is a well-known herb that helps in combating allergy effectively. It has anti-inflammatory and anti-allergic activity. It helps in faster wound healing and has blood-purifying properties. It also acts as a carminative, stomachic, appetizer and tonic.. INSTRUCTIONS. Take exactly as recommended by your Health Provider.. If you miss a dose of Haridra and you are using it regularly, use it as soon as possible. Do not use 2 doses at once.. DOSAGE. Take 1 capsule twice a day after meals.. STORAGE. Store at the room temperature away from moisture and sunlight. Keep out of reach of children.. SAFETY INFORMATION. There are no know precautions for using Haridra.. SIDE EFFECTS. Haridra isnt known to have any side effects if taken as per the prescribed dosage. ...

Description: Histamine is a mediator of the allergic response. Histamine release causes itching, flushing, hives, vomiting, syncope, and even shock. In addition, some patients with gastric carcinoids may exhibit high concentrations of histamine ...

Choosing to participate in a study is an important personal decision. Talk with your doctor and family members or friends about deciding to join a study. To learn more about this study, you or your doctor may contact the study research staff using the contacts provided below. For general information, Learn About Clinical Studies. ...

Antigen combine with antibody. Mast cells & basophils release chemotactic agents. Histamine release causes vessel dilation and. Contraction of smooth muscles Result in increased capillary permeability and constriction of bronchioles ...

Basophils are a type of granulocyte named for their propensity for binding basic dyes. They also possess large granules filled with histadine, proteolytic enzymes, leukotrienes, and proteoglycans. Basophils help generate inflammatory reactions in response to IgE and can be recruited for allergic responses. Human basophils can often be phenotyped cells that express 2D7 antigen, CD123, CD203c, FceRIa (they do not express CD117). Mouse basophils can be identified by expression of CD200R3 and FceRIa. BioLegend develops and manufactures world-class, cutting-edge immunological reagents for biomedical research, offered at an outstanding value.

Quercetin Maintains histamine release and degranulation Quercetin has been shown to be one of the most bioactive flavonoids. Quercetin maintains healthy histimine production by supporting healthy enzyme activity. Supplement Facts: Amount Per Serving each vegetable capsule contains: quercetin 250 mg. Directions: 2-4 capsules per day, in divided doses, between meals.

Quercetin Maintains histamine release and degranulation Quercetin has been shown to be one of the most bioactive flavonoids. Quercetin maintains healthy histimine production by supporting healthy enzyme activity. Supplement Facts: Amount Per Serving each vegetable capsule contains: quercetin 250 mg. Directions: 2-4 capsules per day, in divided doses, between meals.

Most reactions to Codeine not allergic but side-effects of codeine/narcotics (gi upset, constipiation, sedation, itching, hives). Codeine directly causes histamine release without the participation of allergic antibodies. Also, sulfa allergy is only to sulfa antibiotics, not other types of sulfas, e.G diurectics, diabetes meds. Would you like to video or text chat with me? ...