While most of the information available concerns the numerous effects of thyroid hormones on the maturation of neurons, it has been noted that glial cells also are affected by thyroid status (1). In...
Sry-related box (Sox) transcription factors share a conserved high-mobility-group box domain (HMG-domain) that binds DNA in the minor groove and bends DNA for further assembly of transcriptional machineries. During organogenesis, each member of the Sox family triggers a specific cell lineage differentiation, indicating that their interactions with DNA are different from each other. Therefore, investigating structural rearrangement of each Sox transcription factor HMG-domain upon binding to DNA would help to elucidate the distinctive molecular mechanism by which they interact with DNA. Previous studies have determined the crystal structures of Sox2 HMG-domain/DNA, Sox4 HMG-domain/DNA, Sox9 HMG-domain/DNA and Sox17 HMG-domain/DNA complexes. However, major gaps remain in the structural information on the Sox transcription factor HMG-domains. Here, we report the crystal structure of the human Sox17 HMG-domain alone at 2.4 A resolution. Comparing this structure and the structure of the mouse Sox17 ...
HMG20B; high mobility group 20B; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily E member 1-related; BRAF25; BRAF35; HMG box domain containing 2; HMGX2; HMGXB2; SMARCE1r; SOXL; SMARCE1-related protein; high-mobility grou; high-mobility group 20B; BRCA2-associated factor 35; HMG box-containing protein 20B; HMG domain-containing protein 2; Sox-like transcriptional factor; HMG domain-containing protein HMGX2; structural DNA-binding protein BRAF35; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily E, member 1-related; PP7706; pp8857; FLJ26127; SOXL, HMGX2, BRAF25, BRAF35, HMGXB2, PP7706, pp8857, SMARCE1r; fc85b02; wu:fc85b02; zgc:110001; si:dkey-110c1.6; high mobility group box domain containing ...
TY - JOUR. T1 - Alleviation of historic H1-mediated transcriptional repression and chromatin compaction by the acidic activation region in chromosomal protein HMG-14. AU - Ding, Hanfei. AU - Bustin, Michael. AU - Hansen, Ulla. PY - 1997/10/1. Y1 - 1997/10/1. N2 - Histone H1 promotes the generation of a condensed, transcriptionally inactive, higher-order chromatin structure. Consequently, historic H1 activity must be antagonized in order to convert chromatin to a transcriptionally competent, more extended structure. Using simian virus 40 minichromosomes as a model system, we now demonstrate that the nonhistoric chromosomal protein HMG-14, which is known to preferentially associate with active chromatin, completely alleviates historic H1-mediated inhibition of transcription by RNA polymerase II. HMG-14 also partially disrupts histone H1-dependent compaction of chromatin. Both the transcriptional enhancement and chromatin-unfolding activities of HMG-14 are mediated through its acidic, C-terminal ...
Non-essential INO80 Chromatin Remodeling Complex Subunit; Preferentially Binds DNA Ends, Protecting Them From Exonucleatic Cleavage; Deletion Affects Telomere Maintenance Via Recombination; Related To Mammalian High Mobility Group Proteins
Protein Related To Mammalian High Mobility Group (HMG) Proteins; Nuclear Protein; Essential For Function Of H/ACA-type SnoRNPs, Which Are Involved In 18S RRNA Processing
In article ,2834 at alsys1.aecom.yu.edu,, ,burk at alsys1.aecom.yu.edu., writes: , There has been considerable interest and work on the evolution of , proteins between species. Does anyone know of work on analyses of , promoter sequences between species for a given gene? Not to blow my own horn too much. ;-} Take a look at my paper: Evolutionary Conserved Motifs and Protein Binding Elements in the 5 Region of the Chromosomal Protein HMG-14 Gene. DNA and Cell Biology, 12(8):753-761. IMHO, comparitive sequence analysis between promoter elements is the best way of identifying regions that important for promoter function ...
High mobility group (HMG)I proteins bind preferentially to the minor groove of A.T-rich regions in double-stranded DNA [1,2]. DNA-binding of these, and several related, proteins is effected by an 11-residue domain known as an A.T-hook [1]. Within known HMG-I proteins are found three highly conserved regions, closely related to the consensus sequence TPKRPRGRPKK [1]. A synthetic oligopeptide with this sequence specifically binds to substrate DNA in a manner reminiscent of intact HMG-I proteins. Structure predictions suggest that the peptide has a secondary structure similar to the anti-tumour and anti-viral drugs netropsin and distamycin, and to the dye Hoechst 33258 [1]. These ligands, which also preferentially bind to A.T-rich DNA, effectively compete with both the synthetic peptide and the HMG-I proteins for DNA binding [1]. The peptide also contains novel structural features such as a predicted Asx bend, or "hook", at its N-terminus, and laterally-projecting cationic Arg/Lys "bristles", which ...
A high mobility group protein (HMGB1, 215 aa) considered a structural protein in chromatin. It can be released from endotoxin or cytokine-stimulated macrophages and is toxic (levels are high in patients with sepsis). It is bound by RAGE (receptor for advanced glycation end products ) and inhibition of the RAGE-amphoterin interaction suppresses various systems linked to tumour proliferation, invasion, and expression of matrix metalloproteinases. ...
File Title: Active Chromatin Structure of Saccharomycea cerevisiae: High Mobility Group Proteins, Histone Modifications and DNase I Sensitivity ...
Authors: Buchko, Garry; Ni, Shuisong; Reeves, Raymond; Kennedy, Michael. Citation: Buchko, Garry; Ni, Shuisong; Lourette, Natacha; Reeves, Raymond; Kennedy, Michael. "NMR resonance assignments of the human high mobility group protein HMGA1." J. Biomol. NMR 38, 185-185 (2007).. Assembly members: ...
The high mobility group (HMG) proteins I and Y are well characterized nonhistone chromosomal proteins which bind to A·T-rich regions of DNA, and may regulate gene expression and/or DNA replication. We utilized a series of mouse mammary epithelial preneoplastic and tumor cell lines to explore the relationship between neoplastic transformation and HMG-I(Y) gene expression. The cell lines used in this study were originally derived from a single hyperplastic outgrowth, and exhibit a distinct gradient of preneoplastic to highly metastatic transformation states. We measured the levels of HMG-I(Y) gene expression in these cell lines during the different phases of cell growth in culture. At both subconfluent and confluent cell densities, elevated levels of HMG-I(Y) mRNA were directly correlated with the relative degree of neoplastic transformation and metastatic progression of these cells. HMG-I(Y) mRNA levels were always highest in proliferating cells. However, the differences in HMG-I(Y) gene ...
The SOX18 gene localizes to the long arm of chromosome 20. It spans a length of about 3.3 Kb and encodes a protein of 468 amino acids. Like all other SOX genes, SOX18 also carries a characteristic conserved DNA sequence that codes for an approximately 80 amino acid DNA binding domain having homology with the High Mobility Group (HMG) box domain. Another vital domain in Sox18 is the transactivation domain, which is a 92 amino acid domain immediately C terminal of the HMG DNA binding region. Only a handful of mutations in SOX18 leading to HLTS are known. These include both missense as well as nonsense mutations. The missense mutations tend to affect the HMG box and result in a phenotype with a dominant mode of transmission. The nonsense mutations, on the other hand, tend to terminate the transactivation domain, and result in a phenotype that is transmitted in an autosomal recessive manner. ...
The SOX18 gene localizes to the long arm of chromosome 20. It spans a length of about 3.3 Kb and encodes a protein of 468 amino acids. Like all other SOX genes, SOX18 also carries a characteristic conserved DNA sequence that codes for an approximately 80 amino acid DNA binding domain having homology with the High Mobility Group (HMG) box domain. Another vital domain in Sox18 is the transactivation domain, which is a 92 amino acid domain immediately C terminal of the HMG DNA binding region. Only a handful of mutations in SOX18 leading to HLTS are known. These include both missense as well as nonsense mutations. The missense mutations tend to affect the HMG box and result in a phenotype with a dominant mode of transmission. The nonsense mutations, on the other hand, tend to terminate the transactivation domain, and result in a phenotype that is transmitted in an autosomal recessive manner. ...
TY - JOUR. T1 - Contributions of high mobility group box protein in experimental and clinical acute lung injury. AU - Ueno, Hiroshi. AU - Matsuda, Tomoyuki. AU - Hashimoto, Satoru. AU - Amaya, Fumimasa. AU - Kitamura, Yoshihiro. AU - Tanaka, Masaki. AU - Kobayash, Atsuko. AU - Maruyama, Ikuro. AU - Yamada, Shingo. AU - Hasegawa, Naoki. AU - Soejima, Junko. AU - Koh, Hidefumi. AU - Ishizaka, Akitoshi. PY - 2004/12/15. Y1 - 2004/12/15. N2 - This study was performed to examine the putative role of high mobility group box (HMGB) protein in the pathogenesis of acute lung injury (ALI). Observations were made (7) in 21 patients who were septic with ALI and 15 patients with normal lung function and (2) in a mouse model 24 hours after intratracheal instillation of lipopolysaccharide (LPS). The concentrations of HMGB1 were increased in plasma and lung epithelial lining fluid of patients with ALI and mice instilled with LPS. LPS-induced ALI was mitigated by anti-HMGB1 antibody. Although this protein was ...
high mobility group nucleosome-binding domain-containing protein 2,high mobility group protein N2,high-mobility group (nonhistone chromosomal) protein 17,high-mobility group nucleosomal binding domain 2,non-histone chromosomal protein HMG-17,nonhistone chromosomal protein HMG-17 ...
REPORT High Mobility Group Protein B1 (High Mobility Group Protein 1 or High Mobility Group Protein Box 1 or HMGB1) - High mobility group box 1 protein also known as high-mobility group protein 1 (HMG-1) is a protein encoded by ... > ...
PREDICTED: similar to High mobility group protein 1 (HMG-1) (High mobility group protein B1) (Amphoterin) (Heparin-binding protein p30) isoform 1 [Canis familiaris ...
Nuclear high-mobility-group (HMG) proteins were isolated from hen oviduct. These were proteins HMG-1, −2, −3, −14 and −17, which are equivalent to the classification of calf thymus HMG proteins. Hen oviduct proteins HMG-1 and −2 were individually isolated by HCIO4.extraction and CM-Sephadex chromatographic separation. Their mol.wts. were determined as 28 000 and 27 000, respectively. The proteins have a high content of acidic and basic amino acids. The association of proteins HMG-1 and −2 with the genome of hen oviduct nuclei was probed by a limited digestion with nucleases. Hen oviduct nuclei were incubated with deoxyribonuclease I or micrococcal nuclease until 10% of the DNA was digested. The nuclear suspension was centrifuged and the contents of proteins HMG-1 and −2 in the supernatant and sediment fractions were analysed by polyacrylamide-gel electrophoresis. HMG proteins were found to be preferentially released by micrococcal-nuclease digestion rather than by deoxyribonuclease ...
Background: Smokers show increased prevalence of insulin resistance for glucose and type 2 diabetes mellitus (T2DM). The underlying mechanisms, however, remain poorly understood. Tobacco smoke exposure increases tissue expression of high mobility group box 1 (HMGB1), a damage-associated molecular pattern (DAMP) that activates innate immunity. We recently reported that exposure of human macrophages to tobacco smoke extract (TSE) provokes the release of HMGB1 in two forms: as a soluble molecule and carried on membrane microvesicles (MV). Both soluble recombinant HMGB1 (rHMGB1) and MV-associated HMGB1 on TSE-induced MVs (TSE-MVs) mediate crucial crosstalk with adipocytes by impairing insulin signaling and by recruiting monocytes to the adipocytes -. GOAL- To identify the receptors on adipocytes that mediate the effects of HMGB1 on insulin signaling and monocyte recruitment.. Methods: Cultured 3T3-L1 adipocytes were incubated for 24h with rHMGB1 or TSE-MVs, in the absence or presence of neutralizing ...
The high mobility group (HMG) of nuclear proteins regulates expression of many genes through architectural remodelling of the chromatin structure, and formation of multiprotein complexes on promoter/enhancer regions. This leads to the active transcription of their target genes. Here we show that HMGA2, a member of the HMGA sub-family of HMG proteins, has a critical function in cardiogenesis. Overexpression of HMGA2 enhanced, whereas siRNA-mediated knockdown of HMGA2 blocked, cardiomyocyte differentiation of the embryonal carcinoma cell line P19CL6. Moreover, overexpression of a dominant-negative HMGA2 or morpholino-mediated knockdown of HMGA2 expression blocked normal heart formation in Xenopus laevis embryos, suggesting that HMGA2 has an important role in cardiogenesis both in vitro and in vivo. Mechanistically, HMGA2 associated with Smad1/4 and showed synergistic trans-activation of the gene for a cardiac transcription factor Nkx2.5; a conserved HMGA2 binding site was required for the promoter ...
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HMG1 and 2 (high mobility group proteins 1 and 2; renamed HMGB1 and 2) contain two DNA-binding HMG-box domains (A and B) and a long acidic C-terminal domain. They bind DNA without sequence specificity, but have a high affinity for bent or distorted DNA, and bend linear DNA. The individual A and B boxes (which, although broadly similar, show both structural and functional differences) exhibit many of the structure-specific properties of the whole protein. The acidic tail modulates the affinity of the tandem HMG boxes in HMG1 and 2 for a variety of DNA targets, including four-way junctions, but not distorted DNA minicircles, to which the proteins bind with very high affinity. HMG1 and 2 appear to play important architectural roles in the assembly of nucleoprotein complexes in a variety of biological processes, for example V(D)J recombination, the initiation of transcription, and DNA repair. ...
Component of the FACT complex, a general chromatin factor that acts to reorganize nucleosomes. The FACT complex is involved in multiple processes that require DNA as a template such as mRNA elongation, DNA replication and DNA repair. During transcription elongation the FACT complex acts as a histone chaperone that both destabilizes and restores nucleosomal structure. It facilitates the passage of RNA polymerase II and transcription by promoting the dissociation of one histone H2A-H2B dimer from the nucleosome, then subsequently promotes the reestablishment of the nucleosome following the passage of RNA polymerase II. Binds specifically to double-stranded DNA (By similarity).
Hepatoma derived growth factor Hepatoma-derived growth factor (HDGF) also known as high mobility group protein 1-like 2 (HMG-1L2) is a protein that in humans is encoded by the HDGF gene. Hepatoma-derived growth factor (HDGF), a potential predictive and prognostic marker in several human cancers, is the firstly reported
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In this article, we show that c-Jun binds to the HMG-I/Y promoter and induces expression of the HMG-I/Y chromatin binding protein. Decreasing HMG-I/Y proteins using an antisense approach inhibits c-Jun/AP-1-induced transformation, while overexpression of HMG-I/Y confers anchorage-independent cell growth. The colonies in Rat1a cells overexpressing HMG-I/Y were smaller and fewer, suggesting that HMG-I/Y expression only partially recapitulates the c-Jun/AP-1 phenotype. HMG-I/Y is a chromatin remodeling protein with DNA binding domains called AT hooks, which bind to the minor groove of stretches of AT-rich DNA (13-21, 26). Several studies suggest an important role for HMG-I/Y in regulating gene transcription (13-26, 42-44). The HMG-I/Y gene is highly conserved among species; the transcribed regions of the human and mouse HMG-I/Y genes are ∼80% identical at the nucleotide sequence level and ,90% identical when only the protein-coding exons are considered (48). Both mouse and human HMG-I/Y promoters ...
These reference sequences exist independently of genome builds. Explain. These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above. ...
In order to determine the biological functions of moderately abundant, high mobility group (HMG)-like nuclear proteins, a genetic approach has been taken. The gene for one such protein, NHP2, has been cloned and characterized from Saccharomyces cerevisiae. NHP2 has been called HMG-like because of the physical/chemical properties it shares with the HMG proteins from higher eukaryotic cells. However, nucleotide sequence analysis revealed that NHP2 could encode a 17.1 kilodalton basic protein which was not significantly homologous to any previously sequenced HMG proteins. Thus NHP2 defines a new member of the HMG class of proteins. A search of protein databases showed that the amino acid sequence of NHP2 shared significant identities with two ribosomal proteins; the acidic ribosomal protein S6 from Halobacterium marismorium and protein L7a from mammals. The biological relevance of these homologies is unclear since previous biochemical results indicated that NHP2 was not a ribosomal protein. S1 ...
SP100B, a binding domain required during gene repression, especially during viral infections. It is only found during cell stress and after it interacts with an interferon. With the addition of SAND and high mobility group (HMG) interaction domain that serve as a DNA binding domain. It can then regulate transcription from chromosomal genes such as AIRE-1 (autoimmune regulator 1) and NUDR (nuclear DEAF-1 related). In a study done by Wilcox, et.al (2005) ,ref, Wilcox, K., Sheriff, S., Isaac, A., & Taylor, J. (2005). SP100B is a repressor of gene expression. Journal of cellular biochemistry, 95(2).,/ref,, it was shown that SP100B plays roles during the repression from an activated promoter and represses basal promoter activity by inhibiting the transactivation of ICP4, hence repressing both promoters. The required domains for repression were predicted to lie on the C-terminal of B domain that contains HMG and SAND expression that interact with HP1 domain ...
SP100B, a binding domain required during gene repression, especially during viral infections. It is only found during cell stress and after it interacts with an interferon. With the addition of SAND and high mobility group (HMG) interaction domain that serve as a DNA binding domain. It can then regulate transcription from chromosomal genes such as AIRE-1 (autoimmune regulator 1) and NUDR (nuclear DEAF-1 related). In a study done by Wilcox, et.al (2005) ,ref, Wilcox, K., Sheriff, S., Isaac, A., & Taylor, J. (2005). SP100B is a repressor of gene expression. Journal of cellular biochemistry, 95(2).,/ref,, it was shown that SP100B plays roles during the repression from an activated promoter and represses basal promoter activity by inhibiting the transactivation of ICP4, hence repressing both promoters. The required domains for repression were predicted to lie on the C-terminal of B domain that contains HMG and SAND expression that interact with HP1 domain ...
Clone REA701 recognizes the human Sox17 antigen, a member of the SRY-related HMG-box family of transcription factors, which are implicated in embryogenesis. Sox17 is a transcription regulator that binds target promoter DNA and bends the DNA. Sox17 is found in adult heart, lung, spleen, testis, ovary, placenta, fetal lung, and kidney. In normal gastrointestinal tract, it is preferentially expressed in esophagus, stomach, and small intestine than in colon and rectum. Additional information: Clone REA701 displays negligible binding to Fc receptors. - Nederland
Clone REA701 recognizes the human Sox17 antigen, a member of the SRY-related HMG-box family of transcription factors, which are implicated in embryogenesis. Sox17 is a transcription regulator that binds target promoter DNA and bends the DNA. Sox17 is found in adult heart, lung, spleen, testis, ovary, placenta, fetal lung, and kidney. In normal gastrointestinal tract, it is preferentially expressed in esophagus, stomach, and small intestine than in colon and rectum. Additional information: Clone REA701 displays negligible binding to Fc receptors. - Schweiz
Electrophoretic concentration (EC) is an electric field-driven and environmentally friendly off-line sample preparation for charged analytes. EC was demonstrated for the enrichment of either six anionic pollutants or five cationic drugs from purified, drinking, river, or waste- water samples. EC provided analyte enrichment in 15-50 min with concentration factors of 30-249 and 12-243 for the negatively and positively charged analytes, respectively. A modification of the EC device enabled simultaneous EC and separation (SECS) of six cationic and anionic herbicides with concentration factors of 18-337 in 30 min. The potential of SECS has also been evaluated for the determination of high mobility ions in urine and the results obtained have been compared to common acetonitrile treatment of urine. SECS provided an enrichment of high mobility ions and revealed more peaks compared to the acetonitrile treatment ...
The newest playable character to join the ranks of Paladins: Champions of the Realm has been announced to be Talus, of the Skadrin.
Background: High Mobility Group Box 1 (HMGB1) is as an abundant and ubiquitous nuclear DNA-binding protein that has multiple functions dependent on its cellular location. In the nucleus, HMGB1 binds to DNA and is critical for proper transcriptional regulation. However, little is known about the effects of HMGB1 on heart failure. The aim of this study was to examine the impact of HMGB1 on cardiac dysfunction induced by pressure overload.. Methods and Results: We generated transgenic mice with cardiac-specific overexpression of HMGB1 (HMGB1-TG) using α-myosin heavy chain promoter. There were no differences in cardiac morphology and function between wild-type (WT) and HMGB1-TG mice at basal condition. Thoracic transverse aortic constriction (TAC) was performed in transgenic mice with cardiac-specific overexpression of HMGB1 (HMGB1-TG) and wild-type (WT) mice. The survival rate after TAC was significantly higher in HMGB1-TG mice compared to WT mice. Increases in heart/body weight ratio after TAC ...
Human malignant gliomas, the most common primary brain tumors, are characterized by excessive proliferation and infiltrative growth. Although the molecular determinants of glioma properties are still unclear, a significant role for the altered regulation of modulators of glial growth and motility has been proposed. In the complex network of molecular signals involved in tumorigenesis, high mobility group box 1 protein (HMGB1) has recently emerged. This low molecular weight protein, previously considered solely as a chromatin-associated molecule, can be present extracellularly after passive release by necrotic cells or active secretion by some cell types. In the extracellular milieu, HMGB1 can act as a multifunctional paracrine/autocrine factor via its interaction with the receptor for advanced glycation end products (RAGE). Increasing evidence supports that HMGB1 plays an important role in tumors, promoting cancer cell growth, motility, and invasion. Although different human tumors overexpress ...
The majority of glutathione, an important antioxidant, appears in the body in its reduced form (GSH), with 10% appearing in its oxidized form of glutathione disulfide (GSSG). A deficiency in glutathione results in oxidative stress and plays a major role in the pathogenesis of type 2 diabetes. Furthermore, oxidative stress is an important cause of intestinal epithelial cell death. High mobility group box 1 protein (HMGB1) has been shown to mitigate disease and inhibit apoptosis. This investigation sought to determine the effect of HMGB1 on oxidative stress in the gut to determine HMGB1s role in the pathogenesis of type 2 diabetes. The oxidative stress of HMGB1 f/f and HMGB1 f/f vil-CRE mice was measured using a GSH/GSSG ratio assay kit on ileal mucosal scrapings, cecal contents, stoop samples, and tissue taken from the liver. Future investigations could investigate the role of HMGB1 in conjunction with diet by also using mice that are fed either a high fat diet or a regular chow diet.
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InterPro provides functional analysis of proteins by classifying them into families and predicting domains and important sites. We combine protein signatures from a number of member databases into a single searchable resource, capitalising on their individual strengths to produce a powerful integrated database and diagnostic tool.
Download Caffeine-induced alterations in non-histone chromosomal proteins of Chinese hamster ovary cells ebook by Susan Claire HarrisonType: pdf, ePub, zip, txt
Sox proteins encompass an evolutionarily conserved family of transcription factors with critical roles in animal development and stem cell biology. In common with vertebrates, the Drosophila group B proteins SoxNeuro and Dichaete are involved in central nervous system development, where they play both similar and unique roles in gene regulation. Sox genes show extensive functional redundancy across metazoans, but the molecular basis underpinning functional compensation mechanisms at the genomic level are currently unknown. Using a combination of genome-wide binding analysis and gene expression profiling, we show that SoxNeuro directs embryonic neural development from the early specification of neuroblasts through to the terminal differentiation of neurons and glia. To address the issue of functional redundancy and compensation at a genomic level, we compare SoxNeuro and Dichaete binding, identifying common and independent binding events in wild-type conditions, as well as instances of compensation and
Background The inflammatory response triggered by cardiac surgery with cardiopulmonary bypass (CPB) is a primary cause of postoperative atrial fibrillation (POAF). The objective of this study was to...
Results presented herein provide new insights into the release of HMGB1 by macrophages in cells stimulated by TLR ligands and suggest that the translocation of this protein and apoptosis are closely related processes that may reflect common mechanisms. Thus, as our findings show, stimulation of RAW 264.7 cells by either LPS or poly(I:C) leads to the translocation of HMGB1 from the nucleus into the extracellular milieu under conditions in which the frequency of apoptotic cells increases dramatically; similar results were obtained with primary macrophages cultured from mice. Although the presence of apoptotic cells in cultures of activated cells does not prove that they are the source of HMGB1, the failure of cells stimulated by CpG DNA to release HMGB1 suggests that activation by itself is not sufficient for this process. Thus, for macrophages, it appears that apoptosis is closely associated with events leading to HMGB1 release and, indeed, may be the origin of at least some of the HMGB1 released ...
The SOX genes form a gene family related by homology to the high-mobility group (HMG) box region of the testis-determining gene SRY. We have cloned and sequenced the SOX10 and Sox10 genes from human a
This gene encodes a key mitochondrial transcription factor containing two high mobility group motifs. The encoded protein also functions in mitochondrial DNA replication and repair. Sequence polymorphisms in this gene are associated with Alzheimers and Parkinsons diseases. There are pseudogenes for this gene on chromosomes 6, 7, and 11. Alternative splicing results in multiple transcript variants ...
Transcription factor high‐mobility group box‐containing protein 1 (HBP1) may function as a tumor suppressor in various types of cancer. In a previous study, we ...
Pharmacological preconditioning reduces myocardial infarct size in ischaemia-reperfusion (I-R) injury. Dexmedetomidine, a selective α2 -adrenoceptor agonist, has a proven cardioprotective effect when administered prior to I-R, although the underlying mechanisms for this effect are not fully understood. We evaluated whether dexmedetomidine preconditioning could induce a myocardio-protective effect against I-R injury by inhibiting associated inflammatory processes through downregulation of the high mobility group box 1 (HMGB1)-toll-like receptor 4 (TLR4)-nuclear factor κB (NF-κB) signalling pathway ...
High mobility group box protein-1 promotes cerebral edema after traumatic brain injury via activation of toll-like receptor 4, 2014 ...
Messenger RNA (mRNA) export adaptors play an important role in the transport of mRNA from the nucleus to the cytoplasm. that the histone chaperone FACT specifically binds UIF VE-821 but not REF via the SSRP1 subunit and this interaction is required for recruitment of UIF to mRNA. Together the results indicate that REF and UIF represent key human adaptors for the export of cellular mRNAs via the UAP56-NXF1 pathway. and proteins. Figure?1 Identification and Characterization of UIF By using an antiserum raised to UIF we detected it in extracts from 293T cells as a 37 kDa protein (Figure?1D). The levels of UIF were increased when cells were transfected with a UIF cDNA expression vector and reduced when cells expressed a microRNA (miRNA) targeting UIF messenger RNA (mRNA) indicating that the antibody recognized UIF. We analyzed manifestation of UIF in chick embryos and discovered a widespread manifestation pattern during advancement (Shape?S2). The expression pattern was identical compared to that ...