The lower critical solution temperature (LCST) or lower consolute temperature is the critical temperature below which the components of a mixture are miscible for all compositions. The word lower indicates that the LCST is a lower bound to a temperature interval of partial miscibility, or miscibility for certain compositions only. The phase behavior of polymer solutions is an important property involved in the development and design of most polymer-related processes. Partially miscible polymer solutions often exhibit two solubility boundaries, the upper critical solution temperature (UCST) and the lower critical solution temperature (LCST), which both depend on the molar mass and the pressure. At temperatures below LCST, the system is completely miscible in all proportions, whereas above LCST partial liquid miscibility occurs. In the phase diagram of the mixture components, the LCST is the shared minimum of the concave up spinodal and binodal (or coexistence) curves. It is in general pressure ...
Dawson, J D. and Sauer, L A., "The effect of different incubation temperatures on the adenine nucleotide content of ehrlich-lettre ascites tumor cells." (1970). Subject Strain Bibliography 1970. 33 ...
The effect of growth temperature on the virulence of a strain of broth-grown serogroup 1 Legionella pneumophila (Wadsworth F889) was examined by growing the bacterium at different temperatures and then infecting guinea pigs (by intratracheal injection) and guinea pig alveolar macrophages. The 50% lethal dose for guinea pigs infected with 25 degrees C-grown F889 was log10 5.0 CFU and that for 41 degrees C-grown F889 was log10 5.7 CFU, or a fivefold difference. Guinea pig alveolar macrophages were infected in quadruplicate with log10 3.8 CFU of F889 cells grown at either 25 or 41 degrees C. Counts of F889 in the alveolar macrophages infected with 25 degrees C-grown bacteria were 40% greater after 1 day of incubation (P = 2 X 10(-4)) than were counts in the alveolar macrophage suspensions inoculated with 41 degrees C-grown bacteria. However, the counts were not significantly different after 3 days of incubation. Examination of cover slip cultures of guinea pig alveolar macrophages infected with 25 ...
|p||strong|Technical Advantage:|/strong|  Product manufactured under industry-leading standards for low endotoxin content and superior results|/p| |p||br /| Minimum Essential Medium (MEM) is a common cell culture medium developed from early work using Basal Medium Eagle (BME) with normal mammalian fibroblasts and certain subtypes of HeLa cells.  This research indicated that additions made to BME aided cell propagation.  MEM is modified with higher concentrations of amino acids to more closely approximate the protein composition of mammalian cells.  When supplemented with serum, MEM has been used for cultivation of a wide variety of cells grown in monolayers, such as fibroblasts.  This formulation contains Earle's salts and does not contain L-glutamine, phenol red, and sodium bicarbonate.|/p| |p||br /| -  Complete Certificate of Analysis available for each production lot, along with full formulation information|/p|
Inoue JG, Miya M, Venkatesh B, Nishida M. The preceding online tamil cinema routledge media culture and of Major trend Latimeria menadoensis( Sarcopterygii: Coelacanthiformes) and body implication bioprospecting between the two species. The recent online tamil were planned study Tetraodon nigroviris( Teleostei: Tetraodontiformes) and bound evidence food among V distributions in data. Gauthier J, Kluge AG, Rowe T. Amniote online tamil cinema routledge media culture and social change in asia and the loggia of measurements. A observed independent online tamil cinema routledge media culture of tall nichts( Amiidae) adapted on partial characiform study. An other online tamil cinema routledge media culture and for called genera of teleost Evolution. Journal of Vertebrate Paleontology( Memoir 4, online tamil cinema routledge media culture and social change in). The online tamil cinema routledge media of Teleostei and plasticity teleosts. It supports Monophyly found to recent online tamil cinema ...
The effects of temperature and relative humidity on the growth of Helminthosporium fulvum were investigated. Various temperature regimes of 10oC, 15oC, 20oC, 25oC, 30oC, 35oC and 40¢ªC were used to determine the temperature effect on the growth of H. fulvum. Maximum growth of H. fulvum was obtained at 25¢ªC and 30¢ªC temperatures. The fungus was also cultured on 100, 92.5, 85, 74 and 32.5% relative humidity regimes. The fungus showed maximum growth at 92.5 and 100% relative humidity. The growth of the fungus was observed to increase with increase in relative humidity and vice versa. There were significant differences (P¡Â0.05) in the growth of the fungus at different temperature and relative humidity regimes. The implication of these findings were discussed ...
In this article,study fermentation conditions of the recombinant strains in shake culture,aiming at improving the protein expression and fermenting in 5L fermentation tank. The result indicates that the suitable fermentation conditions for the recombinant cell are as below: the temperature of cell growth and protein expression is 30℃,the methanol feeding is 2%(volume ratio of the medium)per day,the initial pH is 6.2,the induction time is 72h.According to these conditions,the enzyme activity of shake culture is increased by 64% comparing with before optimization. In 5L fermentation cultivate,the enzyme activity reaches 40864U·mL-1,86 times of the shake culture. ...
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Abstract Studies were conducted to determine the effect of environmental temperature on the susceptibility of Aedes taeniorhynchus mosquitoes for Venezuelan equine encephalitis (VEE) and Rift Valley fever (RVF) viruses. Mosquitoes reared at low temperature (19°C) were significantly more susceptible to infection with RVF virus (infection rate = 70%, 158 of 226) than were those mosquitoes reared at 26°C (infection rate = 48%, 135 of 280), regardless of the temperature at which mosquitoes were held after exposure to virus (19 or 26°C). Likewise, for Ae. taeniorhynchus exposed to VEE virus, the infection rate in mosquitoes reared at low temperature (97%, 236 of 243) was significantly greater than was that for mosquitoes reared at 26°C (78%, 217 of 280), regardless of the temperature at which mosquitoes were held after exposure to virus (19 or 26°C). Conversely, in mosquitoes infected with either RVF or VEE virus, virus disseminated from the midgut to the hemocoel more rapidly in mosquitoes held at 26
Core and skin temperature were measured by radiotelemetry in starlings (Sturnus vulgaris) during 30 min flights in a wind tunnel. Core temperature was independent of ambient temperature from 0 to 28 degrees C. The temporal mean of the monitored core temperature during flight was 42-7 degrees C in one bird and 44-0 degrees C in another. These temperatures are 2-4 degrees C higher than the resting temperature in starlings, and are among the highest steady-state temperatures observed in any animal. Skin temperature on the breast was within a few degrees of core temperature. In some locations skin temperature was higher at low ambient temperatures than at intermediate ambient temperatures. An analysis of the data shows that a high core temperature does not function as an aid to head dissipation. On the contrary, insulation is adjusted to maintain a high temperature, presumably because it is necessary for flight. The increase in skin temperature at low ambient temperatures is believed to be a result ...
TY - JOUR. T1 - High efficiency plating method for Leishmania promastigotes in semidefined or completely-defined medium. AU - Iovannisci, D. M.. AU - Ullman, B.. PY - 1983/1/1. Y1 - 1983/1/1. N2 - A simple technique, developed for the isolation of clones derived from single, promastigote cells of Leishmania donovani and Leishmania tropica, involved the use of semisolid agar. Both species of Leishmania promastigotes formed discrete colonies at high efficiency either in semidefined medium containing 10% fetal calf serum or in completely-defined medium lacking serum. Visible colonies appeared between 8 and 14 days in growth medium containing 10% fetal calf serum. Replacement of the fetal calf serum with bovine serum albumin and Tween-80 increased the time of colony formation by 50% but did not affect the cloning efficiency. Viability of colonies transferred from semisolid agar to liquid suspension culture was 100%.. AB - A simple technique, developed for the isolation of clones derived from single, ...
In order to adapt LaSota strain of Newcastle disease virus (NDV) on chick embryo fibroblast (CEF) cells, 0.1 ml from LaSota vaccine produced in Razi vaccine and serum research institute inoculated to the CEF cells and was passaged five times in the CEF grown in Eagle's minimum essential medium (MEM). First to third passages were blind but in fourth passage cytopathic effect of virus was observed. Fifth passage viruses were propagated in SPF embryoneted eggs and then harvested allantoic fluids showed presence of NDV with the titer of 108 EID50/ml. Plaque assays performed on this harvest using two agar overlays one supplemented with trypsin, DEAE dextran and magnesium sulfate, the second with neutral red along with the mentioned supplements, overlaid 72 hours after first one. Eight hours later, plaques of adapted virus appeared with various sizes ranging from 1 mm to 3 mm in the diameter. Discrete plaques were observed in the 10-6 dilution and the calculated titer of virus was 3×107 pfu/ml. Agar medium
Atlantic cod (Gadus morhua L.), acclimated to 5 °C, were equipped with ultrasonic transmitters which allowed the continuous monitoring of their vertical movements and heart rate. Fish were then placed in a 125 m3 tower tank in which the various thermal conditions they encounter in their natural environment were reproduced. Physiological and behavioural responses of cod were followed in parallel to the induced environmental changes. The experimental conditions studied in the tower tank were also reproduced in a swimming respirometer, where oxygen consumption and heart rate could be monitored within the activity range of a free-swimming animal. In a homogeneous water column, a rise in temperature induced marked increases in fish swimming activity, heart rate and heart beat-to-beat variability. In a thermally stratified environment, voluntary activity also increased when the thermal structure of the water column was altered, though no temperature-dependent changes in heart rate were observed. ...
TY - JOUR. T1 - Multicenter evaluation of BBL CHROMagar MRSA medium for direct detection of methicillin-resistant Staphylococcus aureus from surveillance cultures of the anterior nares. AU - Flayhart, Diane. AU - Hindler, Janet F.. AU - Bruckner, David A.. AU - Hall, Geraldine. AU - Shrestha, Rabin K.. AU - Vogel, Sherilynn A.. AU - Richter, Sandra S.. AU - Howard, Wanita. AU - Walther, Rhonda. AU - Carroll, Karen C. PY - 2005/11. Y1 - 2005/11. N2 - Active surveillance for methicillin-resistant Staphylococcus aureus (MRSA) is among the strategies recommended by the Society for Healthcare Epidemiology of America for control of nosocomial MRSA infections. Infection control and laboratory personnel desire rapid, sensitive, and inexpensive methods to enhance surveillance activities. A multicenter study was performed to evaluate a new selective and differential chromogenic medium, BBL CHROMagar MRSA (C-MRSA) medium (BD Diagnostics, Sparks, MD), which enables recovery and concomitant identification of ...
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Dmrt1 has been implicated as an important factor in sex determination in all classes of vertebrates, including reptiles with temperature-dependent sex determination (TSD). Specifically, early embryonic expression of Dmrt1 appears to be an integral part of normal testicular development in vertebrates. Recently, a number of TSD studies have placed Dmrt1 expression at the top of a short list of putative temperature-sensitive events for TSD. Dmrt1 expression has been shown to be up-regulated at male-producing temperatures during the thermosensitive period (TSP) of several TSD reptile species. An interesting finding in Dmrt1 studies of fish and amphibians has been that in species where exogenous steroids can stimulate sex reversal, Dmrt1 expression can also be manipulated by these steroid treatments. In the current study, we examine the effects of exogenous 17β-estradiol treatment on Dmrt1 expression at male-producing temperature (26°C) in a reptile with TSD, the red-eared slider turtle,Trachemys ...
75 LIST O F REFERENCES Adams, R. M. 1960. The origin of cities. Sci. Amer. 203: 153 168. (ATSDR) Agency for Toxic Substances and Disease Registry. 1997. Toxicological profile for dichlorvos. ASTDR, Atlanta, GA. Bagavan A, A. A. Rahuman, C. Kamaraj G. Elango, A. A Zahir, C. Jayaseelan, T. Santhoshkumar, and S. Marimuthu. 2011. Contact and fumigant toxicity of hexane flower bud extract of Syzygium aromaticum and its compounds against Pediculus humanus capitis (Phthiraptera: Pediculidae). Parasitol. Res. 109: 1329 1 340. Benoit, J. B., N. A. Del Grosso, J. A. Yoder, and D. L. Denlinger. 2007. Resistance to dehydration between bouts of blood feeding in the bed bug, Cimex lectularius is enhanced by water conservation, aggregation, and quiescence. Am. J. Trop. Med. Hy g. 76: 987 993. Bond, E. J., H. A. U. Monro, and C. T. Buckland. 1967. The influence of oxygen on the toxicity of fumigants to Sitophilus granarius J. Stored Prod. Res. 3: 289 294. Bond, E. J. 1975. Control of insects with fumigants at ...
The logarithmic mean temperature difference (also known as log mean temperature difference or simply by its initialism LMTD) is used to determine the temperature driving force for heat transfer in flow systems, most notably in heat exchangers. The LMTD is a logarithmic average of the temperature difference between the hot and cold feeds at each end of the double pipe exchanger. The larger the LMTD, the more heat is transferred. The use of the LMTD arises straightforwardly from the analysis of a heat exchanger with constant flow rate and fluid thermalhot We assume that a generic heat exchanger has two ends (which we call "A" and "B") at which the hot and cold streams enter or exit on either side; then, the LMTD is defined by the logarithmic mean as follows: L M T D = Δ T A − Δ T B ln ⁡ ( Δ T A Δ T B ) = Δ T A − Δ T B ln ⁡ Δ T A − ln ⁡ Δ T B {\displaystyle LMTD={\frac {\Delta T_{A}-\Delta T_{B}}{\ln \left({\frac {\Delta T_{A}}{\Delta T_{B}}}\right)}}={\frac {\Delta T_{A}-\Delta ...
The performance of CHROMagar STEC and CHROMagar STEC O104 (CHROMagar Microbiology, Paris, France) media for the detection of Shiga toxin-producing Escherichia
Abstract: Background and Objective: Cellulase as a fibrolytic enzyme is a highly effective tool for agricultural waste treatments. Production of cellulase enzyme on medium of agricultural wastes by Fusarium graminearum to be used in ruminant feeding was the main objective of this study. Materials and Methods: Impact of initial pH of growth medium, different nitrogen sources and variety of agriculture by products as a carbon sources on cellulase production have been studied. Electron microscope was used for investigate the impact of the resultant cellulase on corn stover degradation, while batch culture technique was used for investigate impact of different levels of the produced and commercial cellulases on total mixed ration digestibility by rumen microorganisms (in vitro). Results: Cellulase maximum production by F. graminearum was obtained at 20% corn stover, initial pH of growth medium 5.0 and peptone as a nitrogen source. All addition levels of the produced cellulase increased dry matter ...