TY - JOUR. T1 - Open reading frame 2, encoded by the latency-related gene of bovine herpesvirus 1, has antiapoptotic activity in transiently transfected neuroblastoma cells. AU - Shen, Wenwen. AU - Jones, Clinton. N1 - Copyright: Copyright 2009 Elsevier B.V., All rights reserved.. PY - 2008/11. Y1 - 2008/11. N2 - The latency-related (LR) RNA encoded by bovine herpesvirus 1 is abundantly expressed in the trigeminal ganglia of latently infected calves. Expression of LR proteins is necessary for reactivation from latency and the protection of infected neurons from apoptosis. In this study, we demonstrated that an LR-encoded protein, open reading frame 2 (ORF-2), or ORF-2 fusion proteins encoded by alternatively spliced LR transcripts inhibit cold shock or Fas ligand-induced apoptosis in mouse neuroblastoma (neuro-2A) cells. Frameshift mutants of ORF-2 do not inhibit apoptosis, which suggests that protein expression, not LR RNA expression, mediates the antiapoptotic activity of the LR gene in ...

TY - JOUR. T1 - Dexamethasone treatment of calves latently infected with bovine herpesvirus 1 leads to activation of the bICP0 early promoter, in part by the cellular transcription factor C/EBP-alpha. AU - Workman, Aspen. AU - Perez, Sandra. AU - Doster, Alan. AU - Jones, Clinton. PY - 2009. Y1 - 2009. N2 - Sensory neurons within trigeminal ganglia (TG) are the primary site for bovine herpesvirus 1 (BHV-1) latency. During latency, viral gene expression is restricted to the latency-related (LR) gene and the open reading frame ORF-E. We previously constructed an LR mutant virus that expresses LR RNA but not any of the known LR proteins. In contrast to calves latently infected with wild-type (wt) BHV-1 or the LR rescued virus, the LR mutant virus does not reactivate from latency following dexamethasone (DEX) treatment. In this study, we demonstrated that bICP0, but not bICP4, transcripts were consistently detected in TG of calves infected with the LR mutant or LR rescued virus following DEX ...

α-Herpesvirinae subfamily members, including herpes simplex virus type 1 (HSV-1) and bovine herpes virus 1 (BHV-1), initiate infection in mucosal surfaces. BHV-1 and HSV-1 enter sensory neurons by cell-cell spread where a burst of viral gene expression occurs. When compared to non-neuronal cells, viral gene expression is quickly extinguished in sensory neurons resulting in neuronal survival and latency. The HSV-1 latency associated transcript (LAT), which is abundantly expressed in latently infected neurons, inhibits apoptosis, viral transcription, and productive infection, and directly or indirectly enhances reactivation from latency in small animal models. Three anti-apoptosis genes can be substituted for LAT, which will restore wild type levels of reactivation from latency to a LAT null mutant virus. Two small non-coding RNAs encoded by LAT possess anti-apoptosis functions in transfected cells. The BHV-1 latency related RNA (LR-RNA), like LAT, is abundantly expressed during latency. The LR-RNA

Although bovine herpesvirus-4 (BHV-4), a gammaherpesvirus lacking a clear disease association, has been demonstrated in many tissues during persistent BHV-4 infection, a likely site of virus persistence is in cells of the monocyte/macrophage lineage. To establish an in vitro model of persistent infection potentially useful for examining the molecular mechanisms of BHV-4 persistence/latency, we infected the bovine macrophage cell line BOMAC. Following extensive cell death, surviving cells were found to be persistently infected, maintaining the viral genome over many passages and producing low levels of infectious virus. Although selection was unnecessary for the maintenance of the viral genome, cells persistently infected with recombinant BHV-4 containing a neomycin-resistance gene could be selected with geneticin, thus confirming that persistent BHV-4 infection was compatible with cell survival and replication. Furthermore, persistent BHV-4 infection caused no decrease in the growth rate of BOMAC cells.

A tobacco mosaic virus (TMV)-based vector was utilized for expression of a cytosolic form of the bovine herpesvirus type 1 (BHV-1) protein glycoprotein D (gDc). Nicotiana benthamiana plants were harvested 7 days after inoculation with RNA transcripts derived from the TMV-gDc recombinant virus. Recombinant gDc protein of expected electrophoretic mobility accumulated in inoculated leaves to a concentration of about 20 μg/g of fresh leaf tissue. Oil-based vaccines were formulated with crude foliar extracts to immunize mice parentally. After a single injection, animals developed a sustained and specific response to both the isolated gD and native virus particles. Cattle vaccinated with the same gDc containing extracts developed specific humoral and cellular immune responses directed against both the viral gD and BHV-1 particles. Most importantly, animals vaccinated with the plant-produced gDc showed good levels of protection after challenge with the virulent BHV-1. Virus excretion was drastically reduced

en] In contrast to most gammaherpesviruses, Bovine herpesvirus 4 (BoHV-4) has a broad range of host species both in vitro and in vivo. Several in vitro studies demonstrated that some human cell lines are sensitive or even permissive to BoHV-4. These observations led to the hypothesis that cross-species transmission of BoHV-4 could lead to human infections. In the present study, we investigate the sensitivity of BoHV-4 to neutralization by naïve human sera in order to determine if humans exhibit innate anti-viral activities against this virus. Our results demonstrate that human sera from naïve individuals, in contrast to the sera of naïve subjects from various animal species, neutralize BoHV-4 efficiently. A series of complementary experiments were performed to unravel the mechanism(s) of this neutralization. The data obtained in this study demonstrates that human serum neutralizes BoHV-4 in a complement dependent manner activated by natural antibodies raised against the ...

Persistent infection of macrophages with bovine herpesvirus 4 (BoHV-4) has been proposed to play a secondary causal role, along with bacterial infection, in bovine post-partum metritis. Mechanisms of maintenance of BoHV-4 persistent infection are not understood. We previously generated in vitro models of BoHV-4 persistent infection in human rhadomyosarcoma and bovine macrophage cell lines by drug selection of cells infected with BoHV-4 carrying a drug-resistance marker, and demonstrated circular episomal BoHV-4 genomes. In the present study, we used fluorescent in situ hybridization (FISH) to demonstrate BoHV-4 genomes also integrated into the genomes of these persistently infected cells.

Shown are a section from a mock-infected calf (A) and sections obtained 2 days (B), 4 days (C), 6 days (D), 10 days (E), and 14 days (F) after infection. (A) Western blot analysis comparing the expression of BHV-1 genes following infection of bovine CRIB cells. For intracellular staining, cells were fixed in 1% paraformaldehyde (30 min at room temperature) and then permeabilized with 0.1% saponin. Infected MDBK cells were used as a positive control, while mock-infected APC and MDBK cells were used as negative controls. All MDBK cells expressed high levels of BHV-1 glycoproteins (Fig. ↵* Corresponding author. In contrast to TG from mock-infected calves, ORF2 was readily detected in TG sections of latently infected calves (Fig.. coli containing vector pRSET. Reconstitution of infectious virus from BAC plasmids was obtained by transfection in MDBK cells. Images were acquired by the IVIS system 10 to 20 min after luciferin administration. After 1 h of adsorption at 37°C, cells were rinsed with ...

in Veterinary Microbiology (1996), 53(1-2), 133-41. The cell-mediated immunity (CMI) following bovine herpesvirus 4 (BHV4) infection has been poorly investigated in cattle. The in vivo response measured by a delayed type of hypersensitivity (DTH) assay has ... [more ▼]. The cell-mediated immunity (CMI) following bovine herpesvirus 4 (BHV4) infection has been poorly investigated in cattle. The in vivo response measured by a delayed type of hypersensitivity (DTH) assay has been reported to be positive in only few animals showing serological evidences of BHV4 infection. We have investigated the CMI following BHV4 infection by an in vitro antigen-specific interferon gamma (IFN-gamma) release assay, as an indicator of an actively acquired immunity to BHV4. Our preliminary results using a partially purified antigen suggest that there was a measurable CMI in 75 out of 168 animals (44.4%) originating from a farm with a clinical history and serological evidences (76.3% seropositivity) of BHV4 ...

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Detection of specific antibodies against Glycoprotein E (gE) of the Bovine Herpesvirus Type 1 (BHV-1) in milk sample, by blocking ELISA.

Citation Zhang K, Donovan T, Sucharita S, Brownlie R, Snider M, Tikoo SK, van Drunen Littel-van den Hurk S. 2019. US3 kinase-mediated phosphorylation of tegument protein VP8 plays a critical role in the cellular localization of VP8 and its effect on the lipid metabolism of bovine herpesvirus 1-infected cells. J Virol 93:e02151-18. https://doi.org/10.1128/JVI.02151-18. ...

Utvrđivanje prisustva antitela protiv goveđeg respiratornog sincicijalnog virusa (BRSV), virusa parainfluence 3 (PI3) i goveđeg herpesvirusa 1 (BHV 1) u krvnom serumu junadi primenom indirektne imunoenzimske ...

The present invention relates to infectious bovine rhinotracheitis viruses which fail to produce any functional thymidine kinase as a result of an insertion in the thymidine kinase gene, vaccines against infectious bovine rhinotracheitis containing the same and methods for production and use of same. The present invention also relates to infectious bovine rhinotracheitis-based viral vectors useful for the coexpression of foreign genes.

Looking for Infectious Bovine Rhinotracheitis? Find out information about Infectious Bovine Rhinotracheitis. a viral disease in cattle characterized by general inhibition, inflammation of the mucous membrane of the respiratory tract , and by the development of... Explanation of Infectious Bovine Rhinotracheitis

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Two commercial live virus infectious bovine rhinotracheitis (IBR) vaccines for intranasal administration and an inactivated polyvalent calf pneumonia vaccine were compared for safety and efficacy in calves against experimental IBR infections. All three products were clinically safe for use in young calves; a mild, transient, febrile response was induced by one of the live vaccines. Vaccinal virus was recovered for up to 16 days after vaccination from nasal secretions of all calves given live vaccine. Both live vaccines stimulated a serum neutralising antibody response, but the inactivated vaccine failed to elicit any serological response. Following intranasal challenge four months after the first dose of vaccine, all live virus vaccinates remained systemically healthy. A slight nasal discharge and a few rapidly healing ulcers of the nasal mucosa were the only abnormalities observed. Both the group given the inactivated vaccine and the unvaccinated controls developed clinical IBR with pyrexia, ...

Effective protocols against infectious bovine rhinotracheitis (IBR) in cattle combine a life vaccine which is applied every 6 months.

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Reproduction is a large key to maintaining dairy farm profitability and sustainability. Failure in a reproductive program can inevitably result in increased times of low milk production and increases in culling. The reproductive articles by the authors are aimed at aiding producers in selecting, evaluating, and managing their reproductive programs. We are always looking for new items to write and talk about. Something of interest to you? Let us know by simply going to the Contact Us page!. Publications are ordered by year published.. 2013. Impact of Infectious Bovine Rhinotracheitis (IBR) on Reproduction in Cattle - J Fain. Improving Artificial Insemination Techniques - W Graves and R Smith. 2012. Setting Goals to Improve Breeding Strategies - W Graves. Solving Postpartum Breeding Problems - W Graves. Heat Detection Strategies for Dairy Cattle - W Graves. Dairy Herd Synchronization Programs - W Graves. Dairy Reproduction Benchmarks - J Smith, W Gilson, L Ely, and W Graves. 2011. Improving Dairy ...

PEDRAZA, FJ; SCHILD, AL y ALESSI, AC. Immunohistochemical description of encephalitis in Brazilian cattle naturally infected with bovine herpes virus type 5 (BoHV-5). Arch. med. vet. [online]. 2008, vol.40, n.1, pp.69-75. ISSN 0301-732X. http://dx.doi.org/10.4067/S0301-732X2008000100010.. Twelve cases of viral meningoencephalitis in Brazilian cattle were submitted to immunohistochemical analysis for inflammatory response description. All the cases showed severe neurological signs followed by death. Mild to moderate histological inflammatory changes in the brain and cerebellum characterized the neurological infection showing meningitis, mononuclear perivascular cuffing, gliosis, haemorrhage, and macrophages (gitter cells) accompanying great areas of malacia. None of the cases showed intranuclear inclusion bodies. However, in five of them it was possible to isolate the BoHV-5. In order to collect data to allow the description of the inflammatory response in these cases, brain samples from all of ...

Innate immunity is crucial in the early stages of resistance to novel viral infection. The family of cytokines known as the interferons (IFNs) forms an essential component of this system: they are responsible for signalling that an infection is underway and for promoting an antiviral response in susceptible cells. We construct a spatial stochastic model, parameterized by experimental data and informed by analytic approximation, to capture the dynamics of virus-IFN interaction during in vitro infection of Madin-Darby bovine kidney cell monolayers by Herpes simplex virus 1. The dose dependence of infection progression, subsequent monolayer destruction and IFN-β production are investigated. Implications for in vivo infections, in particular the priming of susceptible cells by IFN-β during infection, are considered. ...

Papers published in the August issue of the Journal of NeuroVirology focus on a variety of research topics, including: toxicity and activities of HIV-1 latency-reversing drugs; transcriptional regulation by the bovine herpesvirus 1 protein ORF2; and predictors and characteristics of seizures in PML survivors. Continue reading JNV - August 2016. ...

Indirect immunofluorescence staining of MDBK cells infected with either BoHV-1, BoHV-5, CpHV-1, CvHV-1, CvHV-2, or the Anlier isolate. Cells were incubated unti

She says, When both sides realize that theres no longer an incentive to engage in improper collection and payment practices, I predict that the demand for IBR will diminish to the point that its mainly being used for $200K hospitalizations and similar bills. One non-monetary advantage that IBR will serve is that if a doctor was truly underpaid, he wont have to wait years until the case in chief is resolved to get what is owed him. Another one is that the Board and the judges will not have to deal with issues that they are in no way equipped to address ...

Specifications, pictures, and pricing on our New Sea-Doo GTX 170 iBR, Stock Number: . Shop B & B Cycles in Victorville, California to find your next Watercraft.

A total of six hundred ninety nine (699) blood samples were collected randomly from cattle population of different cattle farms belonging to rural unorganised sectors and organised cattle farms of seven different districts of West Bengal to study the seroprevalence of infectious bovine rhiniotracheitis (IBR) by Virus Neutralization Test (VNT). The seroprevalence of IBR among the cattle population of West Bengal was estimated to be 48.15% on district-wise study (highest seroprevalence rate of 85.29% in the cattle population of Nadia district and lowest rate of seroprevalence of 20.72% in the cattle population of Jalpaiguri district) and 42.32% on sampling area-wise study. Eighteen (18) serologically positive serum samples were selected as representative samples which were subjected to two fold end point titration. The end point titration of the seropositive samples collected from organised cattle farm of organised sector showed the IBR antibody titre to range from 1: 1 to 1: 8, while that of the samples

Message from the Co- Directors VND Update Equine - Coccidioides sp. infection Bovine - Anaphylaxis; Infectious bovine rhinotracheitis virus (IBRv) Small Ruminant - Yersinia pseudotuberculosis infection (goat) Pig - Malignant catarrhal fever (MCF) Poultry - Inclusion body hepatitis Welcome Dr. Wendi Jackson

TY - JOUR UR - http://lib.ugent.be/catalog/pug01:1153143 ID - pug01:1153143 LA - eng TI - Comparative analysis of replication characteristics of BoHV-1 subtypes in bovine respiratory and genital mucosa explants: a phylogenetic enlightenment PY - 2011 JO - (2011) VETERINARY RESEARCH SN - 0928-4249 PB - 2011 AU - Steukers, Lennert 002002270865 802000520297 AU - Vandekerckhove, Annelies UGent 802000137250 002000112516 AU - Van Den Broeck, Wim DI03 801000970865 0000-0001-6683-5658 AU - Glorieux, Sarah 001999359855 801001865386 AU - Nauwynck, Hans DI04 801000719877 0000-0001-5470-0713 AB - In general, members of the Alphaherpesvirinae use the epithelium of the upper respiratory and/or genital tract as a preferential site for primary replication. Bovine herpesvirus type 1 (BoHV-1) may replicate at both sites and cause two major clinical entities designated as infectious bovine rhinotracheitis (IBR) and infectious pustular vulvovaginitis/balanoposthitis (IPV/IPB) in cattle. It has been hypothesized ...

in Point Vétérinaire (2000), 31. Bovine herpesvirus type 4 (BHV-4) is a ubiquitous herpesvirus in cattle. It has been isolated from animals showing a wide variety of clinical signs but few of the isolates have proven experimental ... [more ▼]. Bovine herpesvirus type 4 (BHV-4) is a ubiquitous herpesvirus in cattle. It has been isolated from animals showing a wide variety of clinical signs but few of the isolates have proven experimental pathogenicity. Species that are susceptible to BHV-4 include animals other than ruminants, notably cats and, surprisingly, a primate the owl monkey (Aotus trivirgatus). BHV-4 exists as a latent infection in mononuclear cells. In cattle, BHV-4 has been isolated in animals exhibiting ocular and respiratory conditions and it has been found in females with genital tract conditions such as post-partum metritis and vulvovaginitis. There is some epidemiological and experimental evidence that BHV-4 may be a cause of bovine abortion. There is no vaccine available in ...

Another area would be to look at normal non-disease producing viruses in ancient cattle digs like some of the Herpesviruses of cattle, Chase adds. Most every species has its own Herpesviruses; cattle have five. Infectious bovine rhinotracheitis (IBR) was not recognized as a clinical entity until the 1950s, Chase explains. What changed in the non-disease IBR virus to make it become a pathogen? One then could see if those new or different viral genes would be good targets for vaccines or preventive therapies ...

The Sophia-Antipolis Laboratory specialises in the study of diseases of ruminants and bees. It employs nearly thirty people and its research focuses primarily on Q fever, infectious bovine rhinotracheitis (IBR), ruminant pestiviruses, and the major diseases and poisoning phenomena affecting bees.

Global-Regulation Translation of Repeal Of The Bang Disease Act, Of Rinderleukosegesetzes And Of The Federal Act On Combating The Infectious Bovine Rhinotracheitis And The Infectious Pustular Vulvovaginitis

TY - JOUR. T1 - The first immunoglobulin-like domain of porcine nectin-1 is sufficient to confer resistance to pseudorabies virus infection in transgenic mice. AU - Ono, E.. AU - Tomioka, Y.. AU - Watanabe, Y.. AU - Amagai, K.. AU - Taharaguchi, S.. AU - Glenisson, J.. AU - Cherel, P.. PY - 2006/9/1. Y1 - 2006/9/1. N2 - Nectin-1 is an alphaherpesvirus receptor that binds to virion glycoprotein D (gD). Porcine nectin-1 mediates entry of pseudorabies virus (PRV), herpes simplex virus types 1 and 2 (HSV-1 and HSV-2), and bovine herpesvirus type 1 (BHV-1). The gD-binding domain of nectin-1 is the first or N-terminal immunoglobulin (Ig)-like domain of the entire ectodomain. Here, we generated three transgenic mouse lines expressing a fusion protein consisting of the first Ig-like domain of porcine nectin-1 and the Fc portion of porcine IgG1 to assess the antiviral potential of the first Ig-like domain of nectin-1 in vivo. All of the transgenic mouse lines showed significant resistance to PRV ...

Early symptoms usually include bouts of sneezing, eye infection (conjunctivitis) and watery discharge from nose and eyes. Later on, the cat may develop a fever, along with apathy and loss of appetite. This is often followed by a more severe and purulent discharge from the eyes and nose. When herpes virus induced Rhinotracheitis is the cause, the cat usually develops a more severe eye infection, sometimes complicated by corneal ulcerations. A spastic cough will also be added to the mix.. Once the acute phase runs its course, cats remain carriers of the herpes virus for the rest of their lives. The infection may be reactivated when the carrier cat goes through periods of stress later on in life, inducing a mild upper respiratory infection.. ...

In a meta - analysis of 17 studies, it was found that patients which carry Herpes virus type 1 and type 2 are at high risk of developing Atherosclesosis, which is a disease of the heart arteries characterized by the deposition of fatty material on their inner walls.

IBR, R&D company dedicated to developing innovative and proprietary technological actives. IBR unique approaches to anti aging include COLORLESS CAROTENOIDS branded as IBR-Phyto(flu)ene® for topical and nutricosmetics as well as DORMINs that slow aging by slowing down cell proliferation and preserving telomeres

Andromeda is another pretty ribbon-style tangle from Hungarian tangler Lily Moon. Last month Lilys Drogon tangle proved very popular with the community and she has several tangles on the site.. Andromeda is very easy to tangle and the little touch of shading nicely finishes it off.. As Anita Aspfors Westin notes in the comments, Andromeda is Easy to learn and easy to remember!. Lily illustrates the step-by-step instructions for drawing Andromeda here on her blog. Below her steps she features Andromeda in a beautiful Zentangle® tile featuring the Zentangle®-original tangle Well.. As you enjoy any of the tangles on the site, please do leave a comment of thanks and encouragement to show the artists you appreciate them for sharing their creativity to inspire yours.. Check out the tag lilym for more of Lilys tangles on TanglePatterns.com.. ...

TY - JOUR. T1 - Transactivator Protein BICP0 of Bovine Herpesvirus 1 (BHV-1) Is Blocked by Prostaglandin D2 (PGD2), Which Points to a Mechanism for PGD2-Mediated Inhibition of BHV-1 Replication. AU - Saydam, Okay. AU - Abril, Carlos. AU - Vogt, Bernd. AU - Ackermann, Mathias. AU - Schwyzer, Martin. N1 - Copyright: Copyright 2008 Elsevier B.V., All rights reserved.. PY - 2004/4. Y1 - 2004/4. N2 - The immediate-early protein, BICP0, of bovine herpesvirus 1 (BHV-1) transactivates a variety of viral and cellular genes. In a yeast two-hybrid cDNA library screening, we found that lipocalin-type prostaglandin D synthase, which catalyzes the production of prostaglandin D2 (PGD2), is a cellular target of BICP0. We observed that, during wild-type BHV-1 infection, PGD2 levels were increased intracellularly and decreased in the medium. These effects were absent upon infection with recombinant BHV-1 expressing β-galactosidase instead of BICP0 (A2G2). Transient-expression assays showed that BICP0 alone ...

The study was designed to measure the seroprevalence of viral and bacterial diseases: Infectious bovine rhinotracheitis, bovine viral diarrhea, bovine leukemia, bovine parainfluenza, bovine respiratory syncytial disease, brucellosis, and paratuberculosis among bovine of Himachal Pradesh during the year 2013-2015

The ICP0 protein (bICP0) encoded by bovine herpesvirus 1 is the main viral regulatory protein since it stimulates AZD2014 all viral promoters and therefore productive infection. simplex trojan type 1-encoded ICP0 bICP0 decreases interferon response aspect 3 (IRF3) however not IRF7 proteins amounts in transiently transfected cells. The zinc RING sequences and finger close to the C terminus are essential for bICP0-induced degradation of IRF3. A proteasome inhibitor lactacystin interfered with bICP0-induced degradation of AZD2014 IRF3 recommending that bICP0 straight or indirectly goals IRF3 for proteasome-dependent degradation. IRF3 however not IRF7 isnt easily detectable in the nuclei of productively AZD2014 contaminated bovine cells through the past due stages of an infection. In the framework of productive an infection IRF3 and IRF7 are discovered in the nucleus at early situations after infection. At later situations after an infection IRF7 however not IRF3 is detectable in the nuclei of ...

in Journal of virology (2016), 90(4), 2039-51. Carbohydrates play major roles in host-virus interactions. It is therefore not surprising that, during coevolution with their hosts, viruses have developed sophisticated mechanisms to hijack for their ... [more ▼]. Carbohydrates play major roles in host-virus interactions. It is therefore not surprising that, during coevolution with their hosts, viruses have developed sophisticated mechanisms to hijack for their profit different pathways of glycan synthesis. Thus, the Bo17 gene of Bovine herpesvirus 4 (BoHV-4) encodes a homologue of the cellular core 2 protein beta-1,6-N-acetylglucosaminyltransferase-mucin type (C2GnT-M), which is a key player for the synthesis of complex O-glycans. Surprisingly, we show in this study that, as opposed to what is observed for the cellular enzyme, two different mRNAs are encoded by the Bo17 gene of all available BoHV-4 strains. While the first one corresponds to the entire coding sequence of the Bo17 gene, the ...

reacts with polypeptides of approximately 100 kDa in bovine liver, human heart fibroblasts, and Madin-Darby bovine kidney cultured cells (MDBK), but not with any components in the 110-115 kDa range from these sources, or from rat pheochromocytoma cultured cells (PC12), neuroblastoma or astrocytes. ...

Infecções pelos herpesvírus bovinos 1 (BoHV-1) e 5 (BoHV-5) têm sido sistematicamente detectadas em rebanhos bovinos brasileiros. Entretanto, por causa da alta similaridade antigênica entre estes dois vírus e a falta de testes sorológicos específicos, a prevalência de cada um deles é atualmente desconhecida. Com o objetivo de detectar e diferenciar infecções latentes causadas por BoHV-1 e/ou BoHV-5, gânglios trigêmeos de 200 bovinos foram coletados e analisados para a presença de DNA viral. As amostras foram coletadas em um frigorífico localizado no município de Capão do Leão, Rio Grande do Sul. Uma PCR semi quantitativa foi desenvolvida, otimizada e utilizada para detectar o DNA viral em gânglios. Para diferenciar entre infecções por BoHV-1 e BoHV-5, duas nested PCR tipo-específicas foram desenvolvidas e utilizadas. Além disto, as amostras de soros obtidas foram submetidas a testes de neutralização viral (VN) para detecção de anticorpos. O DNA de BoHV-1 e/ou BoHV-5 ...

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The biological characteristics of BoHV-4 make it a good candidate as a gene delivery vector for vaccination purposes. These characteristics include little or no pathogenicity, unlikely oncogenicity, the capability to accommodate large amounts of foreign genetic material, the ability to infect several cell types from different animal species, and the ability to maintain transgene expression in both undifferentiated and differentiated cells. A recombinant bovine herpesvirus 4 (BoHV-4CMV-IgKE2-14ΔTK) expressing an enhanced secreted form of the bovine viral diarrhea virus (BVDV) structural glycoprotein E2 (gE2-14), obtained by the removal of the putative transmembrane domain and addition of a 14 amino acids peptide at its carboxyl terminal and an immunoglobulin K signal peptide to the amino terminal, was successfully constructed using a Recombineering (recombination -mediated genetic engineering) approach on BoHV-4 cloned as bacterial artificial chromosome. The galactokinase - based recombineering system

We aimed to determine the seroprevalence of infection with Neospora caninum, Leptospira, and bovine herpesvirus type 1 and risk factors associated with these infections in water buffaloes in Veracruz State, Mexico. Through a cross-sectional study, 144 water buffaloes (Bubalus bubalis) raised in 5 ranches of Veracruz were examined for anti-N. caninum and anti-bovine herpesvirus type 1 antibodies by enzyme immunoassays, and anti-Leptospira interrogans antibodies by microscopic agglutination test.. Of the 144 buffaloes studied, 35 (24.3%) were positive for N. caninum, 50 (34.7%) for Leptospira, and 83 (57.6%) for bovine herpes virus. The frequencies of leptospiral serovars in buffaloes were as follows: 18.7% for Muenchen (n = 27), 10.4% for Hardjo LT (n = 15), 9.0% for Pyrogenes (n = 13), and 4.8% for Icterohaemorrhagiae (n = 7). Seropositive buffaloes were found in all 5 ranches studied. Logistic regression showed that cohabitation of buffaloes with cows was associated with infection with ...

P01 Kawashima K, Meyerholz DK, Gallup JM, Grubor B, Lazic T, Lehmkuhl HD, Ackermann MR* (USA, Japan) Differential expression of ovine innate immune genes by preterm and neonatal lung epithelia infected with respiratory syncytial virus N*, Wiggers L, Sachse K, Berndt A, Reinhold P (Belgium, Germany) MMP-2 and MMP-9 activity in bronchoalveolar lavage fluid of Chlamydia suis infected pigs Effects of bromhexine on the immune response in calves with experimentally induced bronchopneumonia P04 Kucheryavenko R, Stegniy B, Stetsenko V, Kucheryavenko V (Ukraine) About the situation of Infectious Bovine Rhinotracheitis in Ukraine P05 Spendlove PJ*, Basgallop N, Hodgson JL, Hodgson DR (Australia) Detection of aerosolised ipratropium bromide (ATROVENT®) in equine urine post administration using liquid chromatography mass spectroscopy (LCMS) P06 Spendlove PJ*, Hodgson JL, Hodgson DR (Australia) Comparative efficacy of aerosol therapies for the reduction of airway neutrophilia in horses P07 Klein CC*, Smith ...

Lentinula edodes, known as shiitake, has been utilized as food, as well as, in popular medicine, moreover, compounds isolated from its mycelium and fruiting body have shown several therapeutic properties. The aim of this study was to determine the antiviral activity of aqueous (AqE) and ethanol (EtOHE) extracts and polysaccharide (LeP) from Lentinula edodes in the replication of poliovirus type 1 (PV-1) and bovine herpes virus type 1 (BoHV-1). The time-of-addition assay was performed at the times -2, -1, 0, 1 and 2 h of the infection. The virucidal activity and the inhibition of viral adsorption were also evaluated. Plaque assay was used to monitor antiviral activity throughout. The AqE and LeP were more effective when added at 0 h of infection, however, EtOHE was more effective at the times 1 h and 2 h of the infection. AqE, EtOHE and LeP showed low virucidal activity, and the inhibition of viral adsorption was not significant. The results allowed us to conclude that AqE, EtOHE and LeP act on the

Human Herpes Virus Type 8 HHV-8, an enveloped icosahedral virus that contains a nucleocapsid and double-stranded linear DNA, is intended for research use only. These virus types are sold in 1.0 mL aliquots and are live and infectious. View Human Herpes Virus Type 8 HHV-8 Culture Fluid from ZeptoMetrix.

Specimen collection. Forty-one bovine semen samples were collected from healthy bulls of different breeds using an artificial vagina. The samples were obtained from a company specializing in reproduction programs and artificial insemination, packed in metal-free conical tubes (15 mL), and kept in liquid nitrogen from the time of collection until delivery of the samples, after which the samples were kept in a freezer (-80 °C) until analyses. These whole samples were used to estimate background levels for essential metals in bovine semen.. For the development of analytical methodology, four different bovine semen pools were prepared using sperms that were collected from six ordinary animals.. Preparation of bovine semen samples for direct introduction To evaluate the calibration method in aqueous medium and by matrix-matching, the samples (200 µL) were diluted 1:50 in a solution containing 0.01% v/v Triton® X-100 and 0.5% v/v HNO3. Ir, Rh and Y were added as internal standards to all samples to ...

What Does the FVRCP Vaccine Protect Against? The FVRCP vaccine (often called the distemper vaccine) actually protects against THREE contagious viral infections in cats. Feline Viral Rhinotracheitis: The rhinotracheitis virus (herpesvirus type 1) is a common cause of upper respiratory tract disease in cats. Infection often results in chronic (life-long) infection with intermittent flares of illness.…

Live vaccine containing per dose of 1 ml 105.2 TCID50 Feline viral rhinotracheitis virus; 104.7 pfu Feline calicivirus; 104.2 TCID50 Feline panleucopenia virus.

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This proposal integrates multiple aspects of molecular virology, host-pathogen interactions, and neuronal biology and highlights the skills and expertise that D...