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A respiratory protection program that meets OSHAs 29 CFR 1910.134 and ANSI Z88.2 requirements or European Standard EN 149 must be followed whenever workplace conditions warrant a respirators use. Wear a NIOSH/MSHA or European Standard EN 149 approved full-facepiece airline respirator in the positive pressure mode with emergency escape provisions ...
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OBJECTIVE: To assess the influence of blocking smooth muscle large conductance Ca(2+) -activated K+ channels and voltage-gated K+ channels on the conducted dilation to ACh and isoproterenol. MATERIALS AND METHODS: Rat mesenteric arteries were isolated with a bifurcation, triple-cannulated, pressurized and imaged using confocal microscopy. Phenylephrine was added to the superfusate to generate tone, and agonists perfused into a sidebranch to evoke local dilation and subsequent conducted dilation into the feed artery. RESULTS: Both ACh- and isoproterenol-stimulated local and conducted dilation with similar magnitudes of decay with distance along the feed artery (2000μm: ∼15% maximum dilation). The gap junction uncoupler carbenoxolone prevented both conducted dilation and intercellular spread of dye through gap junctions. IbTx, TEA or 4-AP, blockers of large conductance Ca(2+) -activated K+ channels and voltage-gated K+ channels, did not affect conducted dilation to either agonist. A combination
During the assembly of gap junctions, a hemichannel in the plasma membrane of one cell is thought to align and dock with another in an apposed membrane to form a cell-to-cell channel. We report here on the existence and properties of nonjunctional, plasma membrane connexin43 (Cx43) hemichannels. The opening of the hemichannels was demonstrated by the cellular uptake of 5(6)-carboxyfluorescein from the culture medium when extracellular calcium levels were reduced. Dye uptake exhibited properties similar to those of gap junction channels. For example, using different dyes, the levels of uptake were correlated with molecular size: 5(6)-carboxyfluorescein (approximately 32%), 7-hydroxycoumarin-3-carboxylic acid (approximately 24%), fura-2 (approximately 11%), and fluorescein-dextran (approximately 0.4%). Octanol and heptanol also reduced dye uptake by approximately 50%. Detailed analysis of one clone of Novikoff cells transfected with a Cx43 antisense expression vector revealed a reduction in dye ...
In a previous work, we showed the extension of the microemulsion area towards highly or fully water dilutable systems by adding a short chain alc. as cosolvent, like ethanol, to the system water/sodium oleate/citronellol/limonene. It was possible to convert an anti-percolative system to a percolative one by making the interfacial film more flexible. The question arises if this is a general concept. For this reason, we investigated pseudo-ternary systems water/surfactant/cosurfactant/ethanol/oil with different cosurfactants (1-pentanol, 1-heptanol, dodecanol, Guerbet alcs.) and fatty acid Me ester-rapeseed biodiesel as oil phase. Sodium oleate was used as surfactant. Ethanol was added as cosolvent to enhance the film flexibility and so to increase the microemulsion area. With increasing hydrophobicity of the cosurfactants and the oil and without further addn. of ethanol, only very restricted single phase areas were obtained. However, with a certain amt. of ethanol, sodium oleate, and 1-heptanol a ...
0038] The APR product stream 124 may comprise APR products that include oxygenated intermediates. As used herein, "oxygenated intermediates" generically refers to hydrocarbon compounds having 1 or more carbon atoms and between 1 and 3 oxygen atoms (referred to herein as C1+O1-3 hydrocarbons), such as alcohols, ketones, aldehydes, furans, hydroxy carboxylic acids, carboxylic acids, diols and triols. Preferably, the oxygenated intermediates have from 1 to 6 carbon atoms, or 2 to 6 carbon atoms, or 3 to 6 carbon atoms. Alcohols may include, without limitation, primary, secondary, linear, branched or cyclic C1+alcohols, such as methanol, ethanol, n-propyl alcohol, isopropyl alcohol, butyl alcohol, isobutyl alcohol, butanol, pentanol, cyclopentanol, hexanol, cyclohexanol, 2-methyl-cyclopentanonol, heptanol, octanol, nonanol, decanol, undecanol, dodecanol, and isomers thereof. The ketones may include, without limitation, hydroxyketones, cyclic ketones, diketones, acetone, propanone, 2-oxopropanal, ...
Abstract: : Purpose: To assess feasibility and biocompatibility of a lamellar, non-perforating synthetic cornea implanted in rabbit eyes following a corneal injury. Methods: Corneal vascularisation and scarring was induced in the right eye of 15 rabbits by application of 1-heptanol and complete surgical removal of the limbus. Implantation of an sDSC (7mm diameter, 450µm thick optical zone, 100µm thick outer flange) was performed after 45 ± 5 days. The KPros were implanted with their central optic part positioned on a completely exposed Descemets membrane (DM), the outer flange was located in deep stroma (85%). 3 different materials were tested: hydrophobic PMMA (n=5), hydrophilic HEMA-MMA (n=5) and HEMA-NVP (n=5), with a water content of 34% and 75%, respectively. The corneal surface was covered with a conjunctiva-Tenon flap. Central flap trephination was performed after 63 ± 7 days. Degree of DM vascularisation and scarring was assessed after flap opening and weekly thereafter. Results: ...
Mouse Connexin-23 ELISA Kit;Mouse Cx23 ELISA Kit;Mouse AEY12 ELISA Kit;Mouse D230044M03Rik ELISA Kit;Mouse Gjf1 ELISA Kit;Mouse Gsfaey12 ELISA Kit;Mouse gap junction protein, epsilon 1 ELISA Kit;Mouse gap junction epsilon-1 protein ELISA Kit;Mouse connexin 23 ELISA Kit;Mouse putative gap junction protein connexin Cx43.4 ELISA Kit ...
The work presented in this thesis describes the influence of the endothelium on smooth muscle cells, and how the structure of the internal elastic lamina (IEL) affects this relationship in mesenteric and saphenous arteries. This was enabled by the study of functional and confocal microscopy dye transfer experiments. Normotensive (WKY) and hypertensive (SHR) rats of 12 weeks and 6 months of age were used to assess the effect of hypertension and ageing on endothelial and smooth muscle cell communication. The endothelium-derived hyperpolarising factor (EDHF) response in mesenteric arteries was investigated using wire myography, and the involvement of myoendothelial gap junctions (MEGJs) was assessed using the putative gap junction inhibitor carbenoxolone. Carbenoxolone attenuated the EDHF response in the WKY, suggestive of the involvement of myoendothelial gap junctions in EDHF. In the saphenous artery, incubation with L-NAME and indomethacin abolished the relaxation to ACh, indicating that there ...
Abstract: : Purpose: The physiological barriers of the eye limit drug delivery to the posterior segment of the eye via topical or systemic administration. Intravitreal administration has proven to be safe and effective in the treatment of various vitreoretinal diseases. Elimination of drugs from the vitreous body can be via aqueous drainage or through the retina. Drugs eliminating predominantly through the aqueous pathway have higher half-lives and results in prolonged therapeutic action in the posterior segment of the eye. Therefore it is important to study the effect of physicochemical properties of the drugs on their elimination characteristics from the vitreous. The purpose of this study is to determine the effect of lipophilicity on vitreous disposition of short-chain aliphatic alcohols. Methods: Radiolabeled (C14) methanol, 1-propanol, 1-pentanol, 1-hexanol and 1-heptanol with log Pc values ranging from -0.77 to 2.7 were studied. New Zealand albino rabbits weighing 2-2.5 kg were used. ...
Bisby F.A., Roskov Y.R., Orrell T.M., Nicolson D., Paglinawan L.E., Bailly N., Kirk P.M., Bourgoin T., Baillargeon G., Ouvrard D. (red.) (2011). "Species 2000 & ITIS Catalogue of Life: 2011 Annual Checklist.". Species 2000: Reading, UK.. http://www.catalogueoflife.org/annual-checklist/2011/search/all/key/Culicidae. Diakses pado 24 September 2012. ...
She was very depressed on presentation, reluctant to walk or move. Her breathing was severely laboured and harsh. Her mandibular glands were enlarged, her gums were pale pink/cyanotic Temperature was 39.7. All the joints and muscles were painful to palpate ...
Gap junctions contain intercellular channels that allow direct communication between the cytosolic compartments of adjacent cells. Each gap junction channel is formed by docking of two hemichannels, each containing six connexins, contributed by each neighboring cell. These channels permit the direct transfer of small molecules including ions, amino acids, nucleotides, second messengers and other metabolites between adjacent cells. Gap junctional communication is essential for many physiological events, including embryonic development, electrical coupling, metabolic transport, apoptosis, and tissue homeostasis. Communication through Gap Junction is sensitive to a variety of stimuli, including changes in the level of intracellular Ca2+, pH, transjunctional applied voltage and phosphorylation/dephosphorylation processes. This figure represents the possible activation routes of different protein kinases involved in Cx43 and Cx36 phosphorylation ...
Gap junctions contain intercellular channels that allow direct communication between the cytosolic compartments of adjacent cells. Each gap junction channel is formed by docking of two hemichannels, each containing six connexins, contributed by each neighboring cell. These channels permit the direct transfer of small molecules including ions, amino acids, nucleotides, second messengers and other metabolites between adjacent cells. Gap junctional communication is essential for many physiological events, including embryonic development, electrical coupling, metabolic transport, apoptosis, and tissue homeostasis. Communication through Gap Junction is sensitive to a variety of stimuli, including changes in the level of intracellular Ca2+, pH, transjunctional applied voltage and phosphorylation/dephosphorylation processes. This figure represents the possible activation routes of different protein kinases involved in Cx43 and Cx36 phosphorylation ...
Severn Biotech, Limited SBP0076 - Gap 27 Cx40 - SBP0076 - Gap 27 Cx40 MW: 1289.51 H-Ser-Arg-Pro-Thr-Glu-Lys-Asn-Val-Phe-Ile-Val-OH Analogue of extracellular loop 2 of connexin40. Gap junction blocker. 1mg 5mg
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The BIA tool measures impedance or resistance through the body by attaching electrodes to the hands and feet while the individual is lying down. A small imperceptible signal passes through the body and is able to determine the difference in composition between muscle and fat based on conduction and resistance. Tissues that hold water like muscle are better conductors and allows the current to pass quicker through the body while fat increases resistance and slows the current down. This tool takes into account subcutaneous and visceral fat (the more problematic fat) and through its algorithms determines the factors listed below. The height, weight, age, and waist to hip ratio of the patient are all used as objective markers needed to run the test and to monitor progress.. ...
Calcium waves are propagated among islands of UMR cells. Calcium waves were induced in a subconfluent monolayer of fluo-3-loaded UMR cells. The outline of c
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