TY - JOUR. T1 - The prediction of drug metabolism using scaffold-mediated enhancement of the induced cytochrome P450 activities in fibroblasts by hepatic transcriptional regulators. AU - Chiang, Tsai Shin. AU - Yang, Kai Chiang. AU - Zheng, Shu Kai. AU - Chiou, Ling Ling. AU - Hsu, Wen Ming. AU - Lin, Feng Huei. AU - Huang, Guan Tarn. AU - Lee, Hsuan Shu. PY - 2012/7. Y1 - 2012/7. N2 - A reliable, reproducible, and convenient in vitro platform for drug metabolism determination and toxicity prediction is of tremendous value but still lacking. In the present study, a collection of 24 hepatic transcription factors and nuclear receptors in different combinations were surveyed, and 10 among them were finally selected to induce the expression and enzyme activities of cytochrome P450 (CYP) 3A4, 1B1, and 2C9 in human dermal fibroblasts (HDFs). The expression and activities of these CYPs in the induced HDFs were higher than those in commonly used hepatoma cell lines. High CYP expression and activities ...
Before I began my Hands Free journey, I pushed and pressured and rushed my way through life until I became too busy to breathe. I became too stressed to laugh. I became too distracted to see the precious moments slipping right through my multitasking fingers. Through a painful revelation while out for a run one day, I realized I was missing my life-the best parts of life. I decided to take small steps toward change. I created designated times to use technology so it no longer consumed my time and attention. I lowered my standards from perfect to good enough. I stopped trying to do it all and made sleep a priority again. I began putting my family, my health and my happiness back on the priority list. Its been three and a half years since I began my Hands Free journey. I have not been cured of the Disease of Distraction, but I have made significant progress. I live in today, rather than putting life off for someday. I know my children and my spouse as unique individuals. My smile and laugh have ...
Transcriptional regulation is mediated in large part by posttranslational modifications of chromatin-remodeling enzymes. Wright and colleagues investigated the contribution of poly-(ADP-ribose) polymerase-1 (PARP-1)-driven chromatin modification in hormone-regulated gene expression in the absence of DNA damage, a known activator of PARP-1. Progestin treatment of breast cancer cells led to a rapid and transient increase in PARP-1 activity, as measured by poly-(ADP)-ribosylation (PARylation) of nuclear proteins, and decreased levels of the PARP-1 substrate NAD. In addition, hormone stimulation enhanced the expression of progesterone target genes and induced proliferation, and these effects were dependent on the progestin-driven increase in PARP-1 activity. Inhibition of cyclin-dependent kinase 2 (CDK2), a hormone-activated kinase that regulates progesterone target genes, significantly diminished PAR accumulation and PARP-1-stimulated gene expression in response to progestin, suggesting that CDK2 ...
HNF-3 beta, a member of the HNF-3/fork head family of transcription factors, is expressed in the node, notochord, floor plate, and gut in mouse embryos. A null mutation of this gene leads to embryonic lethality. The primary defect of HNF-3 beta -/- embryos is an absence of organized node and notocho …
I see the father figures of our times who will one day head families and its saddening to see that theres a lot of distraction and misplacement of values these days. The other day I was listening to Inspiration FM and a woman was almost in tears of how she learnt to tolerate her husband who always verbally abused and put her morale down. She made a statement that she had to learn to treat him as who he should be and not what he currently was. Im thinking at the same time and asking myself if I should see Nigeria this way too ...
Purpose: To demonstrate the signaling transduction of TGFbRII→Smads⊣SPDEF→Muc5/ac axis for conjunctival goblet cell differentiation.. Methods: We employed conditional deletion of transforming growth factor β receptor II (TGFβRII) in keratin 14-positive stratified epithelia. Phenotypic and biochemical analyses of the resultant ocular surface were compared to the wild-type littermates at various stages.. Results: We found that, in the absence of an external phenotype, the ocular surface epithelium develops properly, but young mice displayed conjunctival goblet cell expansion, demonstrating that TGFβ signaling is required for normal restriction of goblet cells within the conjunctiva. We observed increased expression of SAM-pointed domain containing ETS transcription factor (SPDEF) in stratified conjunctival epithelial cells in TGFβRII cKO mice, suggesting that TGFβ restricted goblet cell differentiation directly by repressing Spdef transcription. Gain-of-function of Spdef in keratin ...
Hop on to get the meaning of XBF-1 acronym / slang / Abbreviation. The Medical Acronym / Slang XBF-1 means... AcronymsAndSlang. The XBF-1 acronym/abbreviation definition. The XBF-1 meaning is Anterior Neural Plate-specific Winged Helix Transcription Factor. The definition of XBF-1 by AcronymAndSlang.com
Gentaur molecular products has all kinds of products like :search , Prosci \ Checkpoint suppressor 1 is a member of the forkhead_winged helix transcription factor family. Checkpoints are eukaryotic DNA damage-inducible cell cycle arrests at G1 and G2. Checkpoint suppressor 1 s \ 27-724 for more molecular products just contact us
The liver and pancreas are derived from the foregut endoderm. Although we increasingly understand the genetic pathways regulating proliferation and differentiation of these organs once they are specified (McLin and Zorn, 2006; Zaret, 2002; Zhao and Duncan, 2005), the earlier events linking endoderm formation at gastrulation to organ induction are less clear. A detailed understanding of these early developmental decisions is likely to be important for designing efficient strategies to generate therapeutically useful tissue from stem cells.. Hepatic specification in the ventral foregut endoderm requires signals from the surrounding mesenchyme (Fukuda-Taira, 1981; Gualdi et al., 1996; Le Douarin, 1975). In the mouse and chick, these signals include Fgf2 from the cardiogenic mesoderm and BMPs from the septum transversum mesenchyme (Jung et al., 1999; Rossi et al., 2001; Zhang et al., 2004), whereas in zebrafish Wnt2bb in the lateral plate mesoderm is required (Ober et al., 2006). There is also ...
Estrogen inhibition of a key receptor that helps regulate blood pressure may be a new mechanism by which premenopausal women are protected from cardiovascular disease. Abstract The steroid hormone aldosterone (aldo) contributes to cardiovascular disease in animal models and in humans. Aldo activates the mineralocorticoid receptor (MR), a hormone-activated transcription factor, and indeed, pharmacological MR inhibition improves cardiovascular outcomes. Because the incidence of cardiovascular dise
To identify receptor-associated proteins that may contribute to the specificity of insulin and IGF-I signaling responses, a mouse embryo library was screened using the yeast two-hybrid system. Multiple receptor-interactive clones encoding the SH2 domain of the adapter protein Grb10 were isolated. Subsequent cloning of the full-length Grb10 sequence from a mouse fat cDNA library defined a previously unknown Grb10 variant, that appears to be the predominant isoform in mouse tissues. Receptor-deficient R- cells (fibroblasts from mice with homologous disruption of the IGF-I receptor gene) and transfected R- cells expressing either insulin receptors (R-IR cells) or IGF-I receptors (R+ cells) were used to investigate the specificity of Grb10 interaction with the two related receptors. Hormone-activated insulin receptors in R-IR cells coprecipitated with three species, all recognized as Grb10 isoforms by specific Grb10 antibody. Under the same conditions, Grb10 was essentially undetectable in IGF-I ...
The FOXA family of transcription factors regulates chromatin structure and gene expression especially during embryonic development. In normal breast tissue FOXA1 acts throughout mammary development; whereas in breast carcinoma its expression promotes luminal phenotype and correlates with good prognosis. However, the role of FOXA2 has not been previously studied in breast cancer. Our purpose was to analyze the expression of FOXA2 in breast cancer cells, to explore its role in breast cancer stem cells, and to correlate its mRNA expression with clinicopathological features and outcome in a series of patients diagnosed with breast carcinoma. We analyzed FOXA2 mRNA expression in a retrospective cohort of 230 breast cancer patients and in cell lines. We also knocked down FOXA2 mRNA expression by siRNA to determine the impact on cell proliferation and mammospheres formation using a cancer stem cells culture assay. In vitro studies demonstrated higher FOXA2 mRNA expression in Triple-Negative/Basal-like ...
Fibrinogen alpha chain; Cleaved by the protease thrombin to yield monomers which, together with fibrinogen beta (FGB) and fibrinogen gamma (FGG), polymerize to form an insoluble fibrin matrix. Fibrin has a major function in hemostasis as one of the primary components of blood clots. In addition, functions during the early stages of wound repair to stabilize the lesion and guide cell migration during re-epithelialization. Was originally thought to be essential for platelet aggregation, based on in vitro studies using anticoagulated blood. However, subsequent studies have shown that it i [...] (789 aa ...
FOXA3 - FOXA3 - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector shRNA available for purchase from OriGene - Your Gene Company.
References for Abcams Recombinant Human FOXA1 protein (ab98301). Please let us know if you have used this product in your publication
1. ChoH, MuJ, KimJK, ThorvaldsenJL, ChuQ, et al. (2001) Insulin resistance and a diabetes mellitus-like syndrome in mice lacking the protein kinase Akt2 (PKB beta). Science 292: 1728-1731.. 2. TaniguchiCM, KondoT, SajanM, LuoJ, BronsonR, et al. (2006) Divergent regulation of hepatic glucose and lipid metabolism by phosphoinositide 3-kinase via Akt and PKClambda/zeta. Cell Metab 3: 343-353.. 3. WanM, LeavensKF, SalehD, EastonRM, GuertinDA, et al. (2011) Postprandial hepatic lipid metabolism requires signaling through Akt2 independent of the transcription factors FoxA2, FoxO1, and SREBP1c. Cell Metab 14: 516-527.. 4. SaltielAR, KahnCR (2001) Insulin signalling and the regulation of glucose and lipid metabolism. Nature 414: 799-806.. 5. AriasJ, AlbertsAS, BrindleP, ClaretFX, SmealT, et al. (1994) Activation of cAMP and mitogen responsive genes relies on a common nuclear factor. Nature 370: 226-229.. 6. ChriviaJC, KwokRP, LambN, HagiwaraM, MontminyMR, et al. (1993) Phosphorylated CREB binds ...
Zhang, Z., Chu, M., Hu, J., Yi, W., Wang, B., & Ma, X. (2017). P1-Hepatocyte Nuclear Factor 4α Expression Significantly Correlates with the Severity of Chronic Gastritis. International Journal of Clinical and Experimental Pathology, 10 (6). http://dx.doi.org/no doi ...
p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...
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Recombinant protein of human hepatocyte nuclear factor 4, alpha (HNF4A), transcript variant 5, 20 ug available for purchase from OriGene - Your Gene Company.
Hepatocyte nuclear factors (HNFs) are a group of phylogenetically unrelated transcription factors that regulate the transcription of a diverse group of genes into proteins. These proteins include blood clotting factors and in addition, enzymes and transporters involved with glucose, cholesterol, and fatty acid transport and metabolism. As the name suggests, hepatocyte nuclear factors are expressed predominately in the liver. However HNFs are also expressed and play important roles in a number of other tissues so that the name hepatocyte nuclear factor is somewhat misleading. Nevertheless, the liver is the only tissue in which a significant number of different HNFs are expressed at the same time. In addition, there are a number of genes which contain multiple promoter and enhancer regions each regulated by a different HNF. Furthermore, efficient expression of these genes require synergistic activation by multiple HNFs. Hence hepatocyte nuclear factors function to ensure liver specific expression ...
Botanical Name: Matricaria recutita Country of Origin: England Method of Cultivation: Conventional / Field Grown Method of Extraction: Steam Distilled Plant Material Use: dried flower heads Family: Compositae or Asteraceae Synonyms: Blue Chamomile, Chamomilla recutita Flower Oil CAS No: 8002-66-2 Flash Point: 62 degrees C Appearance: Clear Mobile Liquid Color: Dark, blue Call for larger sizes.
Forkhead box protein A1 (FOXA1) is a pioneer factor of estrogen receptor α (ER)–chromatin binding and function, yet the role of FOXA1 in breast cancer and the underlying molecular mechanisms have not yet been elucidated. To evaluate gene expression alterations during breast carcinogenesis, FOXA1 expression was analyzed using the Serial Analysis of Gene Expression Genie suite, a gene expression profiling interactive analysis, and Oncomine analyses. The correlation between methylation and expression was analyzed using the MEXPRESS tool and UCSC Xena browser. Then, the expression and prognostic value of FOXA1 was validated by our own breast cancer samples using RT-PCR. We obtained the following important results. (1) The expression level of FOXA1 was significantly higher in breast cancer than normal tissues. (2) ER, PR, HEGR-2, and nodal status were positively correlated with FOXA1 expression. (3) Among patients with ER+ tumors, those with higher FOXA1
Hepatocyte nuclear factor 4alpha (HNF4alpha) is a regulator of hepatocyte and pancreatic transcription. Hnf4alpha deletion in the mouse is embryonically lethal with severe defects in visceral endoderm formation. It has been concluded in the past that the role of Hnf4alpha in the developing colon was …
HNF4 gamma antibody (hepatocyte nuclear factor 4, gamma) for IHC-P. Anti-HNF4 gamma pAb (GTX70879) is tested in Human samples. 100% Ab-Assurance.
Pcbd1 (GFP-tagged) - Mouse pterin 4 alpha carbinolamine dehydratase/dimerization cofactor of hepatocyte nuclear factor 1 alpha (TCF1) 1 (Pcbd1),, 10 µg.
Complete information for FOXA3 gene (Protein Coding), Forkhead Box A3, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
Purified Recombinant Human FOXA3(T58A) protein, Arginine-tagged from Creative Biomart. Recombinant Human FOXA3(T58A) protein, Arginine-tagged can be used for research.
HNF-4-alpha兔多克隆抗体(ab76649)可与人样本反应并经WB, ELISA实验严格验证。中国75%以上现货,所有产品均提供质保服务,可通过电话、电邮或微信获得本地专属技术支持。
Developmental and Growth Hormone Regulation of the Expression of Liver-Enriched Transcription Factors in the Bovine Liver Satyanarayana Eleswarapu ABSTRACT Liver gene expression changes during development and is affected by growth hormone (GH). These changes in gene expression may be due to the differential expression of the liver-enriched transcription factors (LETFs). To study the potential involvement of LETFs in the regulation of gene expression in the bovine liver, we cloned the cDNA fragments of nine bovine LETFs, including hepatocyte nuclear factor (HNF)-1Æ Ã , 1Æ Ã , 3Æ Ã , 3Æ Ã , 3Æ Ã , 6, albumin D-element binding protein (DBP), and CCAAT/enhancer-binding proteins (C/EBP) -Æ Ã and Æ Ã , and compared the expression levels of them between adult and fetal bovine liver and between GH-treated and untreated adult bovine liver. The mRNA abundance of the LETFs was determined by ribonuclease protection assay (RPA). The cloned bovine LETF cDNA sequences showed high degrees of ...
A family of transcription factors (hepatocyte nuclear factors) enriched in liver. HNF1 is predominantly expressed in liver and kidney and selectively interacts with the control regions of several liver-specific genes. Heterozygous mutations in hepatocyte nuclear factor (HNF)-1α and HNF-1β result in maturity-onset diabetes of the young. ...