Matthew Garrett PharmD Candidate 2015 It is estimated that around 20 million hepatitis E infections occur every year. The hepatitis E virus causes liver disease and is usually transmitted through contaminated drinking water. The highest prevalence of hepatitis E is seen in eastern Asia. Hepatitis E infection usually resolves in 4-6 weeks and is…
This is the case of a 56-year-old man who underwent heart transplantation. Within the first postoperative days, his respiratory and limb muscles weakened, which was attributed to critical illness polyneuromyopathy (CIPM). At day 70 post transplantation, he had increased liver enzyme levels and acute hepatitis E virus (HEV) infection was diagnosed. HEV RNA was found in the serum, stools, and cerebrospinal fluid. Results of further investigations suggested a possible HEV-related polyradiculoneuropathy. At transplantation, the patient was negative for immunoglobulin (Ig)G, IgM, and HEV RNA. A trace-back procedure identified the source of infection and concluded that HEV infection was contracted from blood transfusion 12 days prior to transplantation from an HEV RNA-positive donor. Tests of the organ donor for HEV were negative. Phylogenetic analysis revealed sequence homology between the HEV-3 strain of the patient and the HEV-3 strain of the blood donor. Despite ribavirin treatment, the patient ...
PubMed comprises more than 30 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
Consumption of raw or undercooked pork meat and liver is the most common cause of hepatitis E infection in the EU, said EFSA. More than 21,000 cases of hepatitis E infections have been reported in humans over the last 10 years, with an overall 10-fold inc .... ...
The assay was validated using paired oral fluid and serum samples collected from 141 patients who presented either with (n=76) or without (n=65) symptoms of acute viral hepatitis at a clinical diagnostics center in Dhaka, Bangladesh. The sensitivity and specificity of the oral fluid-based immunoassay for HEV IgG (past HEV infection) and HEV IgA (recent HEV infection) antibodies was calculated in reference to Wantais (Beijing Wantai) serum-based HEV enzyme-linked immunosorbent assay (ELISA) kits for IgG and IgM antibodies, respectively.. RESULTS: ...
Hepatitis E infection is usually a self-limiting disease and an important cause of acute hepatitis in tropical and subtropical regions where the virus is endemic. In industrialized countries, sporadic cases of acute hepatitis E virus (HEV) infections have been described and the number of documented autochthonous infections seems to be increasing. We report three sporadic cases of autochthonous hepatitis E infections in Southwestern Germany which presented at our university hospital within two years. All cases were men who presented with acute hepatitis, icterus and elevated liver. In case 1 and case 2, liver biopsy revealed acute hepatitis, both patients were positive for anti-HEV antibodies, case 1 was also positive for HEV RNA with a viral load of 3.0x10(3)copies/ml in serum. In case 3, anti-HEV antibodies were detectable and HEV RNA was detected in serum (4.3x10(3)copies/ml) and stool (1.4x10(6)copies/ml). None of the patients had a recent travel history outside Germany and close contact to ...
Rabbits have also been suggested as a zoonotic source of Hepatitis E Virus. French researchers found that farmed and wild rabbits in France are naturally infected with Hepatitis E Virus. They also characterized a human Hepatitis E Virus strain that is closely related to rabbit Hepatitis E Virus strains; this finding thus supports the potential of zoonotic transmission from rabbits to humans[3]. However, research showed that isolates from farmed, wild and pet rabbits in The Netherlands were distinct from most human isolates and are unlikely to be a zoonotic source[4] ...
To investigate characteristics of hepatitis E cases in the United States, we tested samples from persons seronegative for acute hepatitis A and B whose clinical specimens were referred to the Centers for Disease Control and Prevention during June 2005-March 2012 for hepatitis E virus (HEV) testing. We found that 26 (17%) of 154 persons tested had hepatitis E. Of these, 15 had not recently traveled abroad (nontravelers), and 11 had (travelers). Compared with travelers, nontravelers were older (median 61 vs. 32 years of age) and more likely to be anicteric (53% vs. 8%); the nontraveler group also had fewer persons of South Asian ethnicity (7% vs. 73%) and more solid-organ transplant recipients (47% vs. 0). HEV genotype 3 was characterized from 8 nontravelers and genotypes 1 or 4 from 4 travelers. Clinicians should consider HEV infection in the differential diagnosis of hepatitis, regardless of patient travel history.
To investigate characteristics of hepatitis E cases in the United States, we tested samples from persons seronegative for acute hepatitis A and B whose clinical specimens were referred to the Centers for Disease Control and Prevention during June 2005-March 2012 for hepatitis E virus (HEV) testing. We found that 26 (17%) of 154 persons tested had hepatitis E. Of these, 15 had not recently traveled abroad (nontravelers), and 11 had (travelers). Compared with travelers, nontravelers were older (median 61 vs. 32 years of age) and more likely to be anicteric (53% vs. 8%); the nontraveler group also had fewer persons of South Asian ethnicity (7% vs. 73%) and more solid-organ transplant recipients (47% vs. 0). HEV genotype 3 was characterized from 8 nontravelers and genotypes 1 or 4 from 4 travelers. Clinicians should consider HEV infection in the differential diagnosis of hepatitis, regardless of patient travel history ...
Read "Hepatitis E virus infection in patients on dialysis and in solid organ transplant recipients in Argentina: exploring associated risk factors, Archives of Virology" on DeepDyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips.
In Brazil, the circulation of hepatitis E virus (HEV) has been demonstrated in distinct groups of individuals and some animals, but its prevalence among individuals with human immunodeficiency virus (HIV) infection is unknown. This study aimed to assess the frequency of serological and molecular HEV markers in individuals infected with HIV from São Paulo, Brazil. Serum and plasma samples of 354 HIV-infected patients collected between 2007 and 2013 were included. All samples were tested for anti-HEV IgG and IgM antibodies and HEV RNA. Anti-HEV IgG and IgM antibodies were detected in 10.7% (38/354) and 1.4% (5/354) of the samples, respectively. Both antibodies were detected simultaneously in only two samples. HEV RNA was not detected in any sample. There was no significant correlation of anti-HEV serological status (positivity to anti-HEV IgG and/or IgM) with sex, age, CD4(+) T cell count, HIV viral load, antiretroviral therapy, liver enzyme levels, or coinfection with hepatitis B virus and/or ...
Discussion: We have reported elsewhere (1) that HEV infection can evolve into chronic hepatitis E in organ transplant recipients receiving immunosuppressive drugs. Two other cases (2, 3) of prolonged HEV infection have been reported: 1 in a patient with lymphoma being treated with rituximab (2), and another in an immunocompromised patient with HIV infection (3). The lymphocyte and CD4+ cell counts were lower than normal in our transplant recipients (1) and in the patient with HIV (3), and they were not reported for the patient with lymphoma (2). Hairy cell leukemia is an indolent chronic B-cell lymphoproliferative disease with a good long-term prognosis, and its main complication is infection (4). We chose to treat this patient with pegylated interferon-α2b for several reasons. We have reported (1) that immunosuppressive drugs are associated with progression to severe liver fibrosis in chronic HEV infection, so we treated this patients chronic HEV infection with pegylated interferon-α2b ...
Chronic hepatitis E virus (HEV) infections have recently been described in HIV-infected patients. Only few data are available for sub-Saharan Africa, where HIV and HEV are highly co-endemic, and where liver pathology is common in HIV-infected individuals. OBJECTIVES: To assess the prevalence of HEV viremia, anti-HEV antibodies, and serum aminotransferase levels in HIV patients in Ghana and Cameroon. STUDY DESIGN: We retrospectively surveyed a cross-section of patients who were enrolled in cohort studies in Ghana (West Africa), and Cameroon (Central Africa). Plasma samples from 1029 HIV patients from Ghana and 515 patients from Cameroon including 214 children were analyzed for HEV-RNA by two reverse transcription PCR methods. In a subset of 791 patients, anti-HEV IgG and IgM antibodies were analyzed. RESULTS: No HEV-RNA was detected in any of the plasma samples of 1544 patients. HEV seroprevalence was high in adult HIV patients from Ghana (45.3%, n=402) and Cameroon (14.2%, n=289), but low in ...
Epidemiological studies found that hepatitis E virus genotype 3 (HEV-3) infection was associated with chronic hepatitis and cirrhosis in immunocompromised patients. Our study aimed to investigate the relationship between the host immunosuppressive status and the occurrence of HEV-related chronic hepatitis. Here we describe a successful experimental study, using cynomolgus monkeys previously treated with tacrolimus, a potent calcineurin inhibitor immunosuppressant, and infected with a Brazilian HEV-3 strain isolated from naturally infected pigs. HEV infected monkeys were followed up during 160 days post infection (dpi) by clinical signs; virological, biochemical and haematological parameters; and liver histopathology. The tacrolimus blood levels were monitored throughout the experiment. Immunosuppression was confirmed by clinical and laboratorial findings, such as: moderate weight loss, alopecia, and herpes virus opportunistic infection. In this study, chronic HEV infection was characterized by the mild
Since 2010, reports of infection with hepatitis E virus (HEV) have increased in England and Wales. Despite mounting evidence regarding the zoonotic potential of porcine HEV, there are limited data on its prevalence in pigs in the United Kingdom. We investigated antibody prevalence, active infection, and virus variation in serum and cecal content samples from 629 pigs at slaughter. Prevalence of antibodies to HEV was 92.8% (584/629), and HEV RNA was detected in 15% of cecal contents (93/629), 3% of plasma samples (22/629), and 2% of both (14/629). However, although HEV is prevalent in pigs in the United Kingdom and viremic pigs are entering the food chain, most (22/23) viral sequences clustered separately from the dominant type seen in humans. Thus, pigs raised in the United Kingdom are unlikely to be the main source of human HEV infections in the United Kingdom. Further research is needed to identify the source of these infections.
Background. In many developed countries hepatitis E virus (HEV) infections have occurred predominantly in travellers to countries endemic for HEV. HEV is a potential threat to blood safety as the virus is transfusion-transmissible. To minimise this risk in Australia, individuals diagnosed with HEV are deferred. Malarial deferrals, when donors are restricted from donating fresh blood components following travel to an area in which malaria is endemic, probably also decrease the HEV risk, by deferring donors who travel to many countries also endemic for HEV. The aim of this study is to describe overseas-acquired HEV cases in Australia, in order to determine whether infection in travellers poses a risk to Australian blood safety. Materials and methods. Details of all notified HEV cases in Australia from 2002 to 2014 were accessed, and importation rates estimated. Countries in which HEV was acquired were compared to those for which donations are restricted following travel because of a malaria risk.
The aim of this study was to analyze the sensitivity of hepatitis E virus antigen (HEV-Ag) to determine acute E hepatitis. Ninety-four serum samples resulting anti-HEV IgM by DIA.PRO assay were analyzed with Wantai assay to check for HEV-Ag. Thirty samples were anti-HEV IgM positive and HEV-RNA positive, 19 samples harbored genotype 3, whereas 11 samples were genotype 1. Overall, 16% of anti-HEV IgM samples resulted HEV-Ag positive and 33.3% of HEV-RNA positive were also HEV-Ag positive. Among 64 HEV-RNA negative samples, 5 (7.8%) were HEV-Ag positive. The concordance of HEV-RNA and HEV-Ag was low (Cohens Kappa=0.36). The Bland-Altman plot revealed a low agreement between HEV-RNA viral load and HEV-Ag, confirmed by a not significant Spearmans correlation coefficient (rho=0.137, p >0.05). Moreover, the HEV-Ag showed 100% specificity. In genotype 3f samples with a viral load >800cp/ml HEV-Ag was positive in 80% of samples, whereas all patients harboring genotype 3e were HEV-Ag-negative ...
... is a viral hepatitis that affects the liver and is caused by the hepatitis E virus, or HEV. The liver is an organ found on the right side of our belly (abdomen) under the rib cage. It cleans our blood of toxins, poisons, and bacteria. It produces bile which helps our body breakdown food. It stores vitamins, minerals and sugars. And it helps control cholesterol levels. Hepatitis E occurs when the hepatitis E virus causes the liver to swell, scar, and become inflamed, which stops it from working properly or completely Symptoms may include nausea, diarrhea, fatigue, and jaundice (yellowing of the skin). Hepatitis E symptoms usually go away after 4-6 weeks. It is rare for HEV to become chronic, but it can be very serious for pregnant women. HEV is spread through food or water that is contaminated with the feces (bowel movement) of people infected with HEV. There is some concern that it may also be passed through pigs in areas where HEV is common. There is no evidence that HEV is passed ...
1) Guillain-Barre syndrome being associated with hepatitis E virus infection and 2) Topic of the month: Review of new oral anticoagulants for stroke. This podcast for the Neurology Journal begins and closes with Dr. Robert Gross, Editor-in-Chief, briefly discussing highlighted articles from the print issue of Neurology. In the second segment Dr. Ted Burns interviews Dr. Bart Jacobs about his paper on Guillain-Barre syndrome being associated with hepatitis E virus infection. Dr. James Addington is reading our e-Pearl of the week about myelin-oligodendrocyte glycoprotein antibody. In the next part of the podcast Dr. Mark McAllister in Read More 1) Guillain-Barre syndrome being associated with hepatitis E virus infection and 2) Topic of the month: Review of new oral anticoagulants for stroke. This podcast for the Neurology Journal begins and closes with Dr. Robert Gross, Editor-in-Chief, briefly discussing highlighted articles from the print issue of Neurology. In the second segment Dr. Ted Burns ...
Hepatitis E Diagnostic Tests Market, Hepatitis E Diagnostic Tests Market Size, Hepatitis E Diagnostic Tests Market Share, Hepatitis E Diagnostic Tests Market Trends, Hepatitis E Diagnostic Tests Market Growth, Hepatitis E Diagnostic Tests Market Research
Hepatitis E virus (HEV) genotype 3, which usually causes asymptomatic infection in Japan, induced severe hepatitis in 8 patients. To better understand genetic features of HEV associated with increased virulence, we determined the complete or near-complete nucleotide sequences of HEV from these 8 patients and from 5 swine infected with genotype 3 strain swJ19. Phylogenetic analysis showed that the isolates from the 8 patients and the 5 swine grouped separately from the other genotype 3 isolates to create a unique cluster, designated JIO. The human JIO-related viruses encoded 18 amino acids different from those of the other HEV genotype 3 strains. One substitution common to almost all human HEV strains in the JIO cluster was located in the helicase domain (V239A) and may be associated with increased virulence. A zoonotic origin of JIO-related viruses is suspected because the isolates from the 5 swine also possessed the signature V239A substitution in helicase ...
Hepatitis E is a disease of major public-health concern mainly in developing countries. Although molecular and sero-epidemiological investigations of HEV have been performed in many provinces in China, the epidemiological data from Yunnan Province are limited and genotypes are not be fully characterized. In this study the prevalence and characteristics of hepatitis E virus (HEV) detected in pigs from Yunnan province, China was evaluated. A total of 13 out of 187 pig fecal samples collected in 2011 revealed HEV positive results; likewise, 7 out of 69 samples collected in 2012 exhibited positive results. These findings indicated a total prevalence of 7.8% (20/256). Phylogenetic and molecular evolutionary analysis results revealed that nine strains were found in the samples obtained in 2011, in which 87.1% to 99.4% nucleotide sequence identity was shared among these strains; and 77.0% to 81.9%, 52.2% to 53.6%, 77.0% to 88.2% and 77.9% to 96.8% nucleotide sequence identities were shared with strains
Infection with hepatitis E virus (HEV) can be clinically inapparent or produce symptoms and signs of hepatitis of varying severity and occasional fatality. This variability in clinical outcomes may reflect differences in host susceptibility or the presence of virally encoded determinants of pathogenicity. Analysis of complete genome sequences supports the division of HEV genotype 3 (HEV-3) variants into three major clades: 3ra comprising HEV isolates from rabbits, and 3efg and 3abchij comprising the corresponding named subtypes derived from humans and pigs. Using this framework, we investigated associations between viral genetic variability of HEV-3 in symptomatic and asymptomatic infections by comparing HEV-3 subgenomic sequences previously obtained from blood donors with those from patients presenting with hepatitis in the UK (54 blood donors, 148 hepatitis patients), the Netherlands (38 blood donors, 119 hepatitis patients), France (24 blood donors, 55 hepatitis patients) and Germany (14 blood donors
Among ten patients who contracted sporadic acute or fulminant hepatitis E between 2001 and 2002 in Hokkaido, Japan, nine (90 %) had a history of consuming grilled or undercooked pig liver 2-8 weeks before the disease onset. We tested packages of raw pig liver sold in grocery stores as food in Hokkaido for the presence of hepatitis E virus (HEV) RNA by RT-PCR. Pig liver specimens from seven (1·9 %) of 363 packages had detectable HEV RNA. Partial sequence analyses revealed that the seven swine HEV isolates belonged to genotype III or IV. One swine HEV isolate (swJL145) from a packaged pig liver had 100 % identity with the HE-JA18 isolate recovered from an 86-year-old patient in Hokkaido. Two swine HEV isolates (swJL234 and swJL325) had 98·5-100 % identity with the HE-JA4 isolate obtained from a 44-year-old patient in Hokkaido. These results indicate that inadequately cooked pig liver may transmit HEV to humans.
TY - JOUR. T1 - Hepatitis e virus (HEV) detection and quantification by a real-time reverse transcription-PCR assay calibrated to the world health organization standard for HEV RNA. AU - Germer, Jeffrey J.. AU - Ankoudinova, Irina. AU - Belousov, Yevgeniy S.. AU - Mahoney, Walt. AU - Dong, Chen. AU - Meng, Jihong. AU - Mandrekar, Jayawant. AU - Yao, Joseph D.. PY - 2017/5/1. Y1 - 2017/5/1. N2 - Hepatitis E virus (HEV) has emerged as a cause of chronic hepatitis among immunocompromised patients. Molecular assays have become important tools for the diagnosis and management of these chronically infected patients. A real-time reverse transcription-quantitative PCR (RT-qPCR) assay utilizing Pleiades probe chemistry and an RNA internal control for the simultaneous detection and quantification of HEV RNA in human serum was developed based on an adaptation of a previously described and broadly reactive primer set targeting the overlapping open reading frame 2/3 (ORF2/3) nucleotide sequence of HEV. A ...
In the present study, we detected anti-HEV IgM and IgG in a cohort of 497 women at mid-term pregnancy and at 6 years postpartum and also in their 497 children. We found that the positive rate of anti-HEV IgM or the prevalence of anti-HEV IgG was constant in women at the two time points. The unchanged positive rates do not reflect that there was no novel HEV infection occurred during the observation period, but it was resulted from the balance of seronegative conversion due to the natural decay of anti-HEV IgG and the seropositive conversion caused by the novel infections. Additionally, we found that anti-HEV prevalence in Chinese children was extremely low.. Previous studies have indicated that commercially available immunoassays for detection of anti-HEV antibodies differ dramatically in the sensitivity and specificity. The sensitivity could range from 72 to 98 % in detecting the same diagnostic sensitivity panel [20], and highly discrepant results existed in evaluation of anti-HEV IgG ...
主要论著:. 1.Shen Q, Pu Y,Fu X, Xie Y, Bian X, Yang S, Yang Y, Cui L, Wang X, Wang H, Zhang W. 2014. Changes in the cellular proteins of A549 infected with hepatitis E virus by proteomics analysis. BMC Vet Res. 2014 Aug 30;10(1):188.. 2.Shen Q, Zhang W, Yang S, Cui L, Hua X. 2012. Complete genome sequence of a new-genotype porcine norovirus isolated from piglets with diarrhea. J Virol. 2012 Jun;86(12):7015-6.. 3.Shen Q, Zhang W, Yang S, Chen Y, Ning H, Shan T, Liu J, Yang Z, Cui L, Zhu J, and Hua X. 2009. Molecular Detection and Prevalence of Porcine Caliciviruses in eastern China from 2008 to 2009. Arch Virol 154(10):1625-30. 4.Shen Q, Zhang W, Cao X, Mou J, Cui L, and Hua X. 2007. Cloning of Full Genome Sequence of Hepatitis E Virus of Shanghai Swine Isolate Using RACE Method. Virol J 4:98. 5.Quan Shen, Rongqing Ren, Wen Zhang, Zhibiao Yang, Shixing Yang, Yan Chen, Li Cui, Xiuguo Hu. 2011. Prevalence of Hepatitis E Virus and Porcine Caliciviruses in Pig Farms of Guizhou Province, China. ...
State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, National Institute of Diagnostics and Vaccine Development in infectious diseases, School of Public Health, Xiamen University, Xiamen, Fujian Province, 361102 China. Along with the improved sensitivity and specificity of Hepatitis E diagnostics, awareness of hepatitis E has increased a lot all over the world. Hepatitis E virus (HEV) is now the leading cause of acute viral hepatitis. In China, the annually reported number of new acute hepatitis E cases gradually increased from 16,444 in 2003 to 29,202 in 2011, and similarly the sales of anti-HEV IgM rose from 378,000 tests in 2003 to 6,096,000 tests in 2011. Although more clinical sites started to consider HEV infection as a diagnosis of hepatitis-like symptoms, the fact that the sales of anti-HEV IgM tests are much lower than those of anti-HAV IgM tests in China and that there is still no commercialized hepatitis E diagnostic reagents on the United States market, would ...
Justification and scope. Hepatitis E virus (HEV) infection is one of the main causes of acute hepatitis in both developed and developing countries. This infectious disease has a high prevalence and incidence in Europe.1 HEV infection has a greater clinical impact in vulnerable populations, such as immunosuppressed patients, pregnant women, and patients with underlying liver disease.2,3 Thus, the World Health Organisation (WHO) ranks it as one of the leading causes of death due to acute hepatitis of viral origin worldwide.4 However, national and international recommendations for the screening, diagnosis, and treatment of HEV have not been developed (EASL guidelines has been reported after submission of the present document),5 which makes it difficult to manage patients. This, combined with the fact that HEV infection is not a notifiable disease in most countries, allows us to speculate that its incidence and clinical impact may be higher than expected. The European Food Safety Authority (EFSA) ...
Abstract We determined the prevalence of IgG antibodies to hepatitis E virus (anti-HEV IgG) among travelers attending Boston-area travel health clinics from 2009 to 2010. Pre-travel samples were available for 1,356 travelers, with paired pre- and post-travel samples for 450 (33%). Eighty of 1,356 (6%) pre-travel samples were positive for anti-HEV IgG. Compared with participants who had never lived in nor traveled to a highly endemic country, the pre-travel prevalence odds ratio (POR) of anti-HEV IgG among participants born in or with a history of previous travel to a highly endemic country was increased (POR = 4.8, 95% CI = 2.3-10.3 and POR = 2.6, 95% CI = 1.4-5.0, respectively). Among participants with previous travel to a highly endemic country, anti-HEV IgG was associated with age | 40 years (POR = 3.7, 95% CI = 1.3-10.2) and travel history to ≥ 3 highly endemic countries (POR = 2.7, 95% CI = 1.2-5.9). Two participants may have contracted HEV infection during their 2009-2010 trip.
Pigs carry hepatitis E virus (HEV) which they can pass on to humans. ...Kunio Satou and Hiroshi Nishiura analysed blood test data from 2500 p...Inoculation studies have shown that the virus remains in pigs?faeces a...HEV which is found worldwide can potentially cause acute hepatitis i...Suckling pigs dont contract the virus in their first 30 days because...,Hepatitis,E,takes,a,piggyback,biological,biology news articles,biology news today,latest biology news,current biology news,biology newsletters
Transmission: like hepatitis A, hepatitis E is mainly transmitted through eating contaminated food or drinking contaminated water. Outbreaks generally occur where there is a lack of safe water and poor sanitation.. Prevention: currently there is a vaccine to prevent hepatitis E, but it is not widely available. Reduce the risk of exposure to hepatitis E by practicing good hygiene and sanitation, and avoiding drinking water that has come from a potentially unsafe source.. Treatment: there is no treatment for hepatitis E but people usually recover by themselves. It can, however, be fatal in some cases. ...
Hepatitis E is an important medical pathogen in many developing countries but is rarely reported from the United States, although antibody to hepatitis E virus anti-HEV is found in1% of U.S. citizens. Zoonotic spread of the virus is suspected. Sera obtained from 239 wild rats trapped in widely separated regions of the United States were tested...
Hepatitis E virus (HEV) is responsible for epidemics and endemics of acute hepatitis in humans, mainly through waterborne, foodborne, and zoonotic transmission routes. HEV is a single-stranded, positive-sense RNA virus classified in the family Hepeviridae and encompasses four known Genotypes (1-4), at least two new putative genotypes of mammalian HEV, and one floating genus of avian HEV. Genotypes 1 and 2 HEVs only affect humans, while Genotypes 3 and 4 are zoonotic and responsible for sporadic and autochthonous infections in both humans and several other animal species worldwide. HEV has an ever-expanding host range and has been identified in numerous animal species. Swine serve as a reservoir species for HEV transmission to humans; however, it is likely that other animal species may also act as reservoirs. HEV poses an important public health concern with cases of the disease definitively linked to handling of infected pigs, consumption of raw and undercooked animal meats, and animal manure ...
This review details the molecular virology of the hepatitis E virus (HEV). a few variants from Africa, and genotypes 3 and 4 include human and swine HEV strains from industrialized countries and Asia (particularly China), respectively. While genotypes 1 and 2 have only been found in humans, genotypes 3 and 4 have been recovered from humans as well as pigs and other animal species. Genotype 3 is usually evenly distributed across the world while genotype 4 is found more often in China and Japan. Early studies on HEV transmission and pathogenesis as well as preclinical vaccine development studies have mostly been carried out in non-human primates such as cynomolgus, rhesus and owl monkeys, and chimpanzees (Uchida et al., 1991; Purdy et al., 1992; Ticehurst et al., 1992; McCaustland et al., 2000). More recently, pigs have also been used for transmission and molecular studies (Meng et al., 1998). However, a small animal model for HEV is still elusive. That and the lack of a suitable cell culture ...
Hepatitis E is becoming a growing health concern in European countries as an increase of sporadic human cases of unknown origin has been recorded lately. Its causative agent, Hepatitis E virus (HEV), is known to have zoonotic potential and thus the role of domestic and wild animals in the chain of v …
Read "A549 and PLC/PRF/5 cells can support the efficient propagation of swine and wild boar hepatitis E virus (HEV) strains: demonstration of HEV infectivity of porcine liver sold as food, Archives of Virology" on DeepDyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips.
ver historia personal en: www.cerasale.com.ar [dado de baja por la Cancillería Argentina por temas políticos, propio de la censura que rige en nuestro medio]// www.revistamedicos.com.ar // www.quorumtuc.com.ar // www.sectorsalud.com.ar // www.maimonides.edu // weblog.maimonides.edu/farmacia/archives/UM_Informe_Autoevaluacion_FyB.pdf - // weblog.maimonides.edu/farmacia/archives/0216_Admin_FarmEcon.pdf - // www.documentalistas.org.ar // www.cpcesfe2.org.ar // www.nogracias.eu // www.estenssorome.com.ar // www.cuautitlan.unam.mx/descargas/licenciaturas/bqd/plandestudio_bqd_ // www.latamjpharm.org/trabajos/25/2/LAJOP_25_2_6_1_M4M6Z9746D.pdf // www.nogracias.eu/v_juventud/informacion/informacionver.asp?cod= // www.colfarse.com.ar // www.proz.com/kudoz/english_to_spanish/art_literary/523942-key_factors.html - 65k - // www.llave.connmed.com.ar/portalnoticias_vernoticia.php?codigonoticia=17715 // www.frusculleda.com.ar/homepage/espanol/activities_teaching.htm // ...
Boutrouille A, Bakkali-Kassimi L, Crucière C, Pavio N. Prevalence of anti-hepatitis E virus antibodies in French blood donors. J Clin Microbiol. 2007; 45(6): 2009-10 ...
Initially, 22-year-old Emilia Indongo thought she was pregnant when she started feeling dizzy, weak, nauseous and started vomiting on Christmas Eve. It was only after 10 days of medical tests, including pregnancy tests, that she was told she is infected with hepatitis E. In the weeks of medical tests a weak Indongo shuttled between her home and hospital, while enduring episodes of vomiting and lying helpless in her bed.
Methods: The HEV open reading frame (ORF)2 protein was purified from plant tissue by using immobilized metal-anion chromatography (IMAC). The recombinant protein was used to develop an in-house ELISA for testing anti-HEV antibodies in both human and swine sera. Thirty-six serum samples collected from patients with serologically proven HEV infection with commercial kits were tested for anti-HEV IgG antibodies by using the plant-expressed protein. Forty-five serum samples collected from apparently healthy pigs in Bulgarian farms were also tested ...
Hepatitis E Virus (HEV) infection is a newly recognized serious threat to global public health and Africa is suspected to be among the most severely affected regions in the world. Understanding HEV epidemiology in Africa will expedite the implementation of evidence-based control policies aimed at preventing the spread of HEV including policies for the use of available resources such as HEV vaccines. Here we present a comprehensive review of HEV epidemiology in Africa based on published data. We searched for articles on HEV epidemiology in Africa from online databases such as PubMed, Scopus, and ISI Web of Science and critically reviewed appropriate publications to extract consistent findings, identify knowledge gaps, and suggest future studies. Taking a particularly high toll in pregnant women and their fetuses, HEV has infected human populations in 28 of 56 African countries. Since 1979, 17 HEV outbreaks have been reported about once every other year from Africa causing a reported 35,300 cases with 650
Hepatitis E virus (HEV) causes acute hepatitis with approximately 20 million cases per year globally. Based on genetic diversity, HEV is classified into different genotypes, with genotype 3 (HEV-3) being most prevalent in Europe and North America. The transmission of HEV-3 has been shown to be zoonotic and mainly associated with the consumption of raw or undercooked pork products. Herein, we investigated the efficacy of high-pressure processing (HPP) in inactivation of HEV-3 using a cell culture system. HPP has been indicated as a promising non-thermal pathogen inactivation strategy for treatment of certain high-risk food commodities, without any noticeable changes in their nature. For this purpose, we treated HEV-3 in media with different conditions of HPP: 400 MPa for 1 and 5 min, as well as 600 MPa for 1 and 5 min, at ambient temperature. All four HPP treatments of HEV in media were observed to result in a 2-log reduction in HEV load, as determined by the amounts of extracellular HEV RNA produced at
Via Eurosurveillance: Hepatitis E in pigs in Israel: seroprevalence, molecular characterisation and potential impact on humans. The abstract: Introduction The zoonotic hepatitis E virus (HEV) genotype 3 (HEV-G3) has become a common cause of acute and chronic hepatitis among humans...
PubMed Central Canada (PMC Canada) provides free access to a stable and permanent online digital archive of full-text, peer-reviewed health and life sciences research publications. It builds on PubMed Central (PMC), the U.S. National Institutes of Health (NIH) free digital archive of biomedical and life sciences journal literature and is a member of the broader PMC International (PMCI) network of e-repositories.
The lack of an efficient cell culture system for hepatitis E virus (HEV) has greatly hampered detailed analyses of this virus. The first efficient cell culture systems for HEV that were developed were
Prevalence and genetic characterization of hepatitis E virus in paired samples of feces and serum from naturally infected pigs.: This study describes the distri
Hepatitis E is a liver disease caused by the hepatitis E virus (HEV). HEV occurs sporadically in developed countries, primarily in the genotypes 3 and 4, which originate in animals. Infection with HEV often progresses in general without symptoms. The infection can also lead to an acute liver infection that often causes more serious complications, especially in pregnant women.
Hepatitis E is a viral infection of the liver that causes inflammation of the organ. The Hepatitis E virus is a positive-sense single-stranded RNA virus with an icosahedral structure comprising a genome of 7.5 kilobases. It was initially classified as belonging to the Caliciviridae family but has since been classified as a member of the Hepeviridae family.
Non-Travel-Associated Hepatitis E in England and Wales: Demographic, Clinical, and Molecular Epidemiological Characteristics. Ijaz, Samreen; Arnold, Eve; Banks, Malcolm; Bendall, Richard P.; Cramp, Matthew E.; Cunningham, Richard; Dalton, Harry R.; Harrison, Tim J.; Hill, Simon F.; MacFarlane, Lorna; Meigh, Rolf E.; Shafi, Shuja; Sheppard, Martin J.; Smithson, Jacquie; Wilson, Melanie P.; Chong-Gee Teo // Journal of Infectious Diseases;10/1/2005, Vol. 192 Issue 7, p1166 Between 1996 and 2003, 186 cases of hepatitis E were serologically diagnosed. Of these, 17 (9%) were not associated with recent travel abroad. Patients were 155 years old (range, 56-82 years old) and tended to be male (76%). Two patients presented with fulminant hepatitis. A total of 129 (69%)... ...