Rabbit antiserum to culture supernatant of concanavalin A-stimulated peripheral blood mononuclear cells was used in a reverse hemolytic plaque assay to detect activated T lymphocytes in human peripheral blood. The number of plaque-forming cells in fresh, unstimulated lymphocyte preparations was approximately 400/10(6) cells, and increased 7- to 14-fold after stimulation with a variety of mitogens and antigens. The kinetics of the increase paralleled 3H-thymidine incorporation, with a maximum on day 3 of culture. Plaques were eliminated by treatment of cells with anti-Leu-I + complement or by depletion of E-rosettes. The activated T cells were not restricted to a given inducer, suppressor, or Ia+ T cell subset, however. Both mitogen-stimulated culture supernatants and mitogen-activated lymphocytes, but not resting lymphocytes, were effective in absorbing the capacity of the rabbit antiserum to develop plaques. This suggested that the predominant specificity of this antiserum was directed toward ...
A reverse haemolytic plaque assay (RHPA) for angiotensinogen was developed in rat hepatoma H4 cells and applied to investigate the possible secretion of angiotensinogen from rat pituitary cells in primary culture. Over a 24-hour incubation period in Cunningham chambers plaques with a mean area of 2,800 +/- 430 and 590 +/- 220 microns2/plaque (SD, n = 6) formed around all viable H4 cells and 2.8 +/- 0.59% of viable pituitary cells respectively. As a positive control PRL secretion from lactotrophs was routinely checked by the RHPA and shown to form plaques with a mean area of 4,050 +/- 1,850 microns2/plaque after a 4-hour incubation. By comparing plaque size in H4 cells with angiotensinogen release in cell culture, as quantified by radioimmunoassay, the secretion rate of angiotensinogen from pituitary cells was calculated as 22 +/- 8 ng/10(6) cells/24 h. Plaque-forming cells consisted of two morphologically distinct populations; 78% being small cells (less than 6 microns diameter) containing little
The hemolytic plaque assay has been adapted to the study of synthesis of C′1 by individual cells. With this method we found that in the guinea pig the only cells capable of producing plaques due to hemolytic C′1 were the columnar epithelial cells of the small intestine. The production of hemolytic plaques was inhibited by puromycin. None of the other cell types studied were capable of producing hemolytic plaques.. ...
We acknowledge the Gadigal people of the Eora Nation, the traditional owners and custodians of the land on which the Garvan Institute of Medical Research is located. We pay respects to Elders, past, present and future, and recognise the continuing connection and contribution to this land. ...
The kinetics of the generation of primed IgM and IgG antibody-forming cell precursors, and of helper T-cell populations, were analyzed in mice whose primary responses to high and low doses of SRBC were arrested at intervals by the immunosuppressive agents cyclophosphamide monohydrate and specific antibody. The extent to which immunological memory was established in these animals before blockade of the primary response was assessed by the hemolytic plaque assay following challenge 12 wk after priming. The presence of IgG B-memory cells and T-memory cells in suppressed mice was further investigated by the transfer into these animals of syngeneic SRBC-stimulated thymocytes or anti-θ-treated spleen cells.. It was found that the progenitors of secondary IgM-synthesizing cells were primed almost immediately after injection of antigen, and that early blockade of the primary response resulted in a raised IgM response after challenge. On the other hand, priming for a secondary IgG response took at least ...
Rihova, B; Tlaskalova, H; and Riha, I, "Detection of mouse cells producing antibodies against the azophenylarsonic group by haemolytic plaque assay." (1980). Subject Strain Bibliography 1980. 259 ...
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Semantic Scholar extracted view of Influence of route and dose of antigen on the migration inhibition and plaque-forming cell responses to sheep erythrocytes in the lizard, Calotes versicolor. by Swaminathan Jayaraman et al.
Spleen cell suspensions of unprimed donor mice containing precursors of immunocytes have been transplanted into X-irradiated recipient mice. In the presence of antigen (sheep erythrocytes) these precursors, called antigen-sensitive units, gave rise to progeny cells secreting specific antibody. We studied quantitatively the production of cells releasing IgM hemolysins (direct plaque-forming cells), IgG hemolysins (indirect plaque-forming cells), and hemagglutinins (cluster-forming cells). We found that each of these immunocyte populations was distinct, i.e., that cells releasing agglutinins did not, as a rule, release hemolysins, and vice versa. We also found that cell populations secreting IgM hemolysins did not shift, under certain experimental conditions, to the production of IgG hemolysins during the primary immune response.. By transplanting graded numbers of spleen cells, we succeeded in limiting to one or a few the number of antigen-sensitive units that reached the recipient spleen. We ...
Severe, or even marginal, deficiencies of zinc lead to reversible dysfunctions of human T lymphocytes.. Zinc deficiency is easily produced in laboratory animals, with a prompt development of numerous abnormalities in immune functions. Zinc deficiency consistently leads to atrophy of the thymus and other lymphoid organs, to reduced lymphocyte numbers in tissues (especially in the T cell areas) and to lymphopenia. Zinc deficiency is accompanied by anergy, manifested predominantly by dysfunctions of cellmediated immunity, along with some depression in the production of T cell-dependent antibodies and splenic plaque-forming cells.. Zinc deficiency initiates a selective decrease in CD4f helper cell numbers. In vitro testing reveals a markedly reduced proliferative response by these cells to phytohemagglutinin and other mitogens. Natural killer cells also show decreased activity and T cell-mediated cytotoxicity is reduced. In zinc-deficiency, lymphocytes have a reduced ability to produce interleukin 2 ...
Definition of THYMUS-DEPENDENT ANTIGEN: T-deyendent antigen. An antigen which fails to stimulate an antibody response if T-lymphocytes are absent. Co-operation between B- lymphocytes and helper T
Baker, P J.; Prescott, B; Stashak, P W.; and Amsbaugh, D F., "Characterization of the antibody response to type iii pneumococcal polysaccharide at the cellular level. Iii. Studies on the average avidity of the antibody produced by specific plaque-forming cells." (1971). Subject Strain Bibliography 1971. 1026 ...
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Plaque assay;a) Sf9 cells negative control (Mock)b) one day after virus dilution added to Sf9 cellsc) two days after virus dilution added to Sf9 cellsd) four da
We have investigated the systemic antibody response to sperm whale myoglobin (SWMb) antigenic sites in three strains of inbred mice using an inhibition of plaque assay. Sperm whale myoglobin was attached to sheep red blood cells (SRBC) via rabbit anti-SRBC Fab fragments. Inhibition of lysis was obtained with synthetic peptides representing the purported five antigenic sites but not with a peptide whose sequence was unrelated to SWMb and synthetic peptides of SWMb from outside the antigenic sites gave minimal or no inhibition. The results of our studies show that the pattern of response to the five antigenic sites differs in each strain, but that almost total inhibition is obtained in all strains with these five sites. The antigenic dominance of these sites supports the concept of discrete antigenic sites on soluble proteins. They also suggest a reason for contrary reports in the literature based on hybridoma technology.
Niels Kaj Jerne FRS (December 23, 1911 - October 7, 1994) was a Danish immunologist. Jerne won the Nobel Prize in Physiology or Medicine with Georges J. F. Köhler and César Milstein in 1957. They won it "[f]or theories concerning the specificity in development and control of the immune system and the discovery of the principle for production of monoclonal antibodies".[1] According to Jernes biographer Thomas Söderqvist, Jerne did not play the role of a bench scientist, he could not pipette accurately, and he did not enjoy experimental work. Jernes Nobel Prize was awarded for theories, rather than discoveries. He developed the "natural selection theory of immunology," which was proposed by Paul Ehrlich 50 years earlier, although he was missing the clonal selection element proposed by David Talmage and later by Frank Macfarlane Burnet. James Watson had told Jerne that his theory "stinks." He was married three times and had two sons, Ivar Jerne (born 1936) and Donald Jerne (born 1941), with ...
There are no specific protocols for Rabbit Anti-Sheep IgM H&L (Alkaline Phosphatase) (ab112761). Please download our general protocols booklet
TY - JOUR. T1 - Suppression and recovery of the immune response and CFU potential of mice during and after chronic low-dose-rate gamma-irradiation. AU - Hubner, Karl. AU - Gengozian, N.. AU - Gilman, T. A.. PY - 1975/1/1. Y1 - 1975/1/1. N2 - Chronic exposure of mice to 60Co γrays at rates of 1·6 R/hr and 6 R/hr leads to profound immunosuppression, as demonstrated with the Jerne haemolysis-in-agar technique. The immunosuppressive effect is dose-rate dependent. Thus, significant reduction of the number of splenic antibody plaque-forming cells (PFC) to sheep red blood cells is seen after exposure to 1005 R at 1·6 R/hr; at the higher exposure rate of 6 R/hr, PFC suppression is seen after a total dose of only 468 R. Although recovery of the PFC response is possible after accumulated doses of 1005, 1579, and 2138 R at 6 R/hr and 5256 R at 1·6 R/hr, normal immunological capacity had not returned 120 days after irradiation. In contrast the recovery of splenic colony-forming units (CFU), as an ...
Looking for online definition of plaque assay in the Medical Dictionary? plaque assay explanation free. What is plaque assay? Meaning of plaque assay medical term. What does plaque assay mean?
BDF1 mice treated with CoV had markedly reduced levels (less than 20%) of native serum C3 32 hr later, whereas the frequency of splenic CR+ cells was normal. CoV treatment before immunization reduced the IgM PFC response to a T-dependent antigen (TNP
... (Anti-Sheep Gold Conjugates). Affinity isolated anti-sheep IgG antibody produced in rabbit and coupled to 50nm gold nanoparticles (0.5ml, OD3).
... (Anti-Sheep Gold Conjugates). Affinity isolated anti-sheep IgG antibody produced in rabbit and coupled to 5nm gold nanoparticles (0.5ml, OD3).
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Anti-Sheep IgA Alkaline Phosphatase secondary antibody validated for WB, ELISA, IHC-P, ICC/IF. Other Alkaline Phosphatase secondaries available.
Plaque-forming bacteria, coloured scanning electron micrograph (SEM). Plaque consists of a film of bacteria embedded in (19527351) Framed, Poster, Canvas Prints, Puzzles, Photo Gifts and Wall Art. Ships from UK, USA, Australia for quick delivery
... Secondary Antibody from Invitrogen for ELISA applications. Supplied as 1 mg purified secondary antibody (4.4 mg/ml) in PBS with 0.09% sodium azide; pH 7.4.
A radial haemolysis test was used to investigate immunity to mumps. Antibody was found in 92 (42%) out of 220 children aged up to 5 years, 124 (78%) out of 159 children aged 6--10 years, 192 (86%) out of 222 children aged 11 years, 138 (92%) out of 150 children aged 15 years, and 280 (95%) out of 296 women attending an antenatal clinic. A group of 307 cadets aged 16--18 years were also tested and interviewed: 133 (95%) out of 140 who said that they had had mumps and 108 (87%) out of 124 who said that they had not had mumps were found to have antibody. The results suggest that tests for immunity to mumps by radial haemolysis would permit more rational use of mumps-specific immunoglobulin and attenuated mumps vaccine. ...
This unit describes the antigenic stimulation of in vitro antibody production by B cells and the subsequent measurement of secreted antibodies. A generalized system for inducing in vitro antibody production is presented along with a procedure for quantifying the number of antibody-producing cells by plaque-forming cell (PFC) assays: the Cunningham-Szenberg technique and the Jerne-Nordin technique. The assay can be modified as described to measure all classes of antibodies or to enumerate total immunoglobulin-secreting B cells. A protocol for preparing the resting B cells by Percoll gradient centrifugation is also described. ...
Female mice were injected with a suspension of 0, 2 ml of 10 per cent v/v sheep red blood cells (SRBC) and sacrificed on the 5th day for recording of spleen mass and assay of plaque-forming cells (PFCs) in the spleens. All PFCs from a pool of five spleens and the individual spleen masses were subjected to rigid statistical analysis to verify whether differences from the control groups were significant (P , 0, 05). Three doses of 4 mg cortisol administered at 24-hourly intervals were given at various times relative to the time of immunization to determine the optimum stage at which to inhibit the formation of PFCs. The greatest inhibitory effect was obtained when the first dose was given at -6 h, with a lesser though still significant depression of PFCs with the -30 h dose regimen. Earlier and later injections had relatively little effect. An attempt was made to determine the response to different dosage levels by injecting doubling doses of steroid starting at the -6 h period. A maximal effect ...
Simpson, M.A.; Gozzo, J.J., 1978: Spectrophotometric determination of lymphocyte mediated sheep red blood cell hemolysis in vitro
Immunologists from Emory University have identified a distinct set of long-lived antibody-producing cells in the human bone marrow that function as an immune archive.
What is the point of a biography? For sure, there are the practical aspects. Writers have to make their way in the world. Readers are naturally curious about famous individuals, particularly those in the same line of work, who usually provide more than their fair share of schadenfreude. Thomas Söderqvist, chair of the Department of History of Medicine at the University of Copenhagen, Denmark, aims higher than this, however; in portraying the life of Niels Kaj Jerne (1911-1994) he hopes to "provide food for ethical reflection for readers in the centres of technoscience who are asking the perennial questions: what is good, what is bad and what constitutes a flourishing life?". As food for ethical reflection, Jerne provides quite a banquet. Tellingly, he saved virtually all his correspondence and manuscript drafts since his school days in anticipation of recounting his glorious life to lesser beings. Söderqvist interviewed Jerne extensively (160 hours of recorded conversations) over several ...
Donkey anti-Sheep IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, HRP conjugate from Invitrogen for Western Blot, Immunohistochemistry and ELISA applications.This antibody is cross-adsorbed against human, mouse and rabbit igg. Supplied as 1 mg purified secondary antibody in PBS with 1% BSA and 0.1% Kathon™ CG; pH 7.2.
TY - JOUR. T1 - The role of B cell proliferation in the generation of immunoglobulin-secreting cells in man. AU - Jelinek, Diane F. AU - Lipsky, P. E.. PY - 1983. Y1 - 1983. N2 - The relationship of B cell proliferation and the generation of immunoglobulin-secreting cells (ISC) was explored in vitro by examining the effect of hydroxyurea (HU), an inhibitor of cellular DNA synthesis, on the generation of ISC from human peripheral blood mononuclear cells (PBM). HU completely inhibited the capacity of PBM to generate ISC in response to pokeweed mitogen (PWM) and other polyclonal B cell activators. Inhibition resulted from an effect on B cell proliferation, because HU also prevented the generation of ISC in cultures of purified B cells supplemented with either T cell supernatants or mitomycin C-treated T cells. Inhibiting B cell proliferation by treating them with mitomycin C before culture also abolished the generation of ISC. When ISC were enumerated after a 7-day incubation with PWM, the addition ...
Rhodamine Conjugated Affinity Purified Anti-SHEEP IgG F(ab)2 (RABBIT), Rhodamine Conjugated Affinity Purified anti-Sheep IgG F(ab )2 [Rabbit]; N/A Rhodamine Conjugated Affinity Purified Anti-SHEEP IgG F(ab)2 (RABBIT)IGHG1
Immune response to immobilized sheep erythrocyte monolayer.: Spleen cells from adult specific pathogen-free miniature swine were exposed to sheep red blood cell
Authors: Claudia Loetsch, Joanna Warren, Adrienne Laskowski, Rodrigo Vazquez-Lombardi, Christoph Jandl, David B Langley, Daniel Christ, David R Thorburn, David K Ryugo, Jonathan Sprent, Marcel Batten, Cecile King
The film is unabashedly pro-sheep and supportive of the sheep lifestyle, with many ewes running about on screen wearing only wool as two cowboys eat badly-cooked provisions.. "Brokeback" was supposed to open last Friday in Sandy, Utah a suburb of Salt Lake City, The AP reports. ...
2017年是(我)變動最大的一年除了全心投入傳統底片行列,在接近2017年的尾聲又迎來了(數位微噴)的新課題,而現今(FB)當道 能存在的網站除了小惡魔01 外大概就我們這個 ...
the antibodies to measles and rubella viruses were titrated in a sample of infantile population in palermo in 1978. measles antibodies were investigated in 900 children aged 0-15 years by the hi test; rubella antibodies in 120 girls aged 12-14 years by the hemolysis-in-gel test. a saturation level of immunity against measles virus as can be found in the adult population appeared to be attained at the age of 12-13 years. at this age however, about 28% of the girls were still not immunized again ...
Peroxidase Conjugated Affinity Purified Anti-SHEEP IgG F(ab)2 (RABBIT), Peroxidase Conjugated Affinity Purified anti-Sheep IgG F(ab )2 [Rabbit]; N/A Peroxidase Conjugated Affinity Purified Anti-SHEEP IgG F(ab)2 (RABBIT)IGHG1
De originale h ngek jer fra Mexico, hvor du b de kan ligge p tv rs, p langs eller diagonalt i h ngek jerne. Jo st rre en h ngek je er, jo mageligere ligger du i h ngek jen. H ngek jen former sig efter kroppens form og er h ndv vet i Mexico. ...
Sera from 1000 women attending antenatal clinics were screened for the presence of rubella antibody by haemagglutination inhibition (HI), single radial haemolysis (SRH), and enzyme-linked immunosorbent assay (ELISA). With 925 sera concordant results were obtained with all three tests. There were 46 false-negative HI tests due to the necessity of allowing for residual non-specific inhibitors. With SRH there was one false positive and three that were negative by SRH but HI- and ELISA-positive. By ELISA 14 sera gave a false-positive result (OD 405 nm greater than or equal to 0.2). Ten of these could be eliminated by taking an OD 405 nm of 0.5 as the threshold but then another 10 sera became false negatives.. ...
Patients with minimal change nephrotic syndrome (MCNS) frequently have suppressed in vivo and in vitro immune responsiveness of uncertain etiology. Because increased suppressor cell activity has been associated with this disease, urines from MCNS patients were screened for activity of the lymphokine soluble immune response suppressor (SIRS), a product of concanavalin A- or interferon-activated suppressor T cells. Urines from untreated MCNS patients suppressed polyclonal plaque-forming cell responses of cultured splenocytes. This suppressive activity was identified as human SIRS by the following functional and physical criteria: molecular weight estimated by gel filtration; kinetics of suppression; inhibition of suppression by catalase, levamisole, and 2-mercaptoethanol; abrogation of activity by acid or protease treatment; elution pattern on high performance liquid chromatography; and cross-reactivity with monoclonal antimurine SIRS antibodies. Suppressive activity disappeared from urine after ...
Anti-Sheep IgG (H&L) rabbit antibody is a polyclonal antibody that targets sheep IgG (H+L). The antibody is conveniently concentrated (5 mg/mL) and contains both heavy and light chains. The antibody can be used as is or conjugated to various enzymes for various immuno-applications. Bulk pricing and biotinylation se
IRVINE, Calif., Nov. 22, 2011 /PRNewswire/ -- AtheroNova Inc. Preclinical Study Demonstrates 95% Reduction in Arterial Plaque Formation. Findings Reported...
Summary Chickens were immunized with eight different serotypes of avian paramyxovirus (PMV). Ninety percent of splenic IgG antibody-forming cells (AFC) were serotype-specific with respect to the glycoprotein and nucleoprotein-polymerase antigens in an indirect immunoperoxidase binding system. The IgG AFCs which cross-reacted between serotypes divided the serotypes into two mutually exclusive super serogroups composed of PMV-1, -3, -4, -7 and -9, and PMV-2, -6 and -8. PMV-5 was not tested.
PubMed Central Canada (PMC Canada) provides free access to a stable and permanent online digital archive of full-text, peer-reviewed health and life sciences research publications. It builds on PubMed Central (PMC), the U.S. National Institutes of Health (NIH) free digital archive of biomedical and life sciences journal literature and is a member of the broader PMC International (PMCI) network of e-repositories.
Methods: Preparation of propofol nanoemulsion using NEMS™ technology has been performed for propofol 1% in NEMS™ (P1%), and propofol 2% in NEMS™ (P2%). Determination of free propofol concentration in aqueous phase was conducted using HPLC and rat paw lick test was evaluated as in vivo test to assay the intensity of pain on injection site. The sleep recovery test was conducted to evaluate the pharmacological effect and erythrocyte hemolysis test also conducted to ensure the safety of propofol in NEMS™. All of the test results were compared with Diprivan®1% as a positive standard. ...
Rabbit anti-sheep IgG recognizes sheep IgM mu heavy chain. This secondary antibody was purified using delipidation and defibrination. Sheep IgM mu heavy chain (PAB9464) - Products - Abnova