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Curcumin is a bifunctional antioxidant derived from |i|Curcuma longa|/i|. This study identifies curcumin as a neuroprotectant against hemin-induced damage in primary cultures of cerebellar granule neurons (CGNs) of rats. Hemin, the oxidized form of heme, is a highly reactive compound that induces cellular injury. Pretreatment of CGNs with 5–30 |i|μ|/i|M curcumin effectively increased by 2.3–4.9 fold heme oxygenase-1 (HO-1) expression and by 5.6–14.3-fold glutathione (GSH) levels. Moreover, 15 |i|μ|/i|M curcumin attenuated by 55% the increase in reactive oxygen species (ROS) production, by 94% the reduction of GSH/glutathione disulfide (GSSG) ratio, and by 49% the cell death induced by hemin. The inhibition of heme oxygenase system or GSH synthesis with tin mesoporphyrin and buthionine sulfoximine, respectively, suppressed the protective effect of curcumin against hemin-induced toxicity. These data strongly suggest that HO-1 and GSH play a
The present study demonstrates a function of HO-1 activity as a potent host defense factor for HIV-1 infection. Our results clearly show that activation of HO-1 by its substrate hemin protected them against HIV infection with various clinical HIV isolates, including some of those that developed resistance to conventional antiretroviral drugs. In vivo, hemin administration in humanized NOD-SCID mice substantially suppressed HIV replication. These findings suggest that the HO-1 inducer, hemin, is a potentially effective endogenous biologic in inducing a host defense response against HIV infection. A critical component of a variety of proteins, including hemoglobin, hemin has been shown to exert numerous beneficial physiological functions (25). After being approved by the Food and Drug Administration, several formulations of hemin have been used since the 1970s to successfully treat acute porphyries, to control liver allograft failure due to recurrence of erythopoietic protophoria, and in patients ...
Hemin is made of red blood cells processed from human blood. Hemin works by lowering the production of a certain enzyme in the body. Hemin is used to treat the symptoms of occasional attacks of porphyria related to the menstrual cycle in women. Hemin helps control symptoms such as pain, increased heart rate or blood...
Different pharmacological and genetic approaches have been used to upregulate HO-1 with the ultimate goal of normalization of BP in SHRs.5,7 The usage of stannous chloride for 4 days (IP), a well-known HO inducer, significantly lowered BP of 7-week-old SHRs but not that of 20-week-old SHRs.6 Treatment with hemin and hemin arginate of 7-week-old SHRs for 4 days (IP) normalized high systolic BP to 130±5 mm Hg.11 Systolic BP of these rats reached 140 mm Hg 1 week later and gradually increased to the levels of untreated SHRs 1 month later.11 All of these previous attempts to combat hypertension by upregulating HO only modestly lowered high BP in young SHRs (5 to 8 weeks old) for an apparently short period of time (3 to 4 weeks).12 Once hypertension is fully established in SHRs (such as in 20-week-old SHRs), upregulating HO expression using a 4-day IP hemin injection failed to lower BP.5,13,14 More importantly, a long-lasting normalization of BP in the absence of antihypertensive interventions has ...
In this study we report the activation of c-Jun N-terminal kinase (JNK) in human K562 erythroleukemia cells undergoing hemin-mediated erythroid differentiation, which occurs concomitantly with activation of heat shock factor 2 (HSF2) and leads to a simultaneous in vivo phosphorylation of c-Jun. The activation of JNK occurs through activation of mitogen-activated protein kinase kinase (MKK) 4 and not by activation of MKK7 or inhibition of JNK-directed phosphatases. We have previously shown that overexpression of the HSF2-beta isoform inhibits the activation of HSF2 upon hemin-induced erythroid differentiation. Here we demonstrate that HSF2-beta overexpression blocks the hemin-induced activation of the MKK4-JNK pathway, suggesting an erythroid lineage-specific JNK activation likely to be regulated by HSF2. ...
Hasan and Schafer demonstrate that hemin-induced Egr-1 expression is derived solely from its ability to induce ROS. Using a panel of ROS scavengers and inhibitors, the authors show that superoxide is necessary for Egr-1 induction because the superoxide scavenger, tiron, completely inhibits hemin-induced ROS. Inhibition of NADPH oxidase activity with apocynin also completely abolishes hemin-induced ROS. Confirmation of these results using alternate approaches such as genetically targeted models or small interfering RNA knockdown will further strengthen the role of NADPH oxidase. These results are significant because they demonstrate that increased ROS levels may be a consequence of the activation of NADPH oxidase by heme and not the result of Fe2+ toxicity. This suggests that heme remains intact in VSM under experimental conditions, eliminating a role for HO-1 and other degradation products, CO and biliverdin/bilirubin, and also excludes the nonenzymatic oxidative degradation of heme and the ...
This paper reviews the evidence that protein synthesis in rabbit reticulocytes is regulated by the reversible phosphorylation of the initiation factor eIF-2 by protein kinases under the control of the cytoplasmic haemin concentration on the one hand, and double-stranded RNA on the other. A molecular mechanism is proposed to account for the observation that inhibition of protein synthesis occurs when considerably less than half the eIF-2 present has been phosphorylated. The question of whether phosphorylation regulates protein synthesis in other types of cell is discussed. ...
s including MARHS4, MARHS2 and MARe in uninduced K562 cells. Hemin induction of the K562 cells obviously increased the binding of SATB1 to MARHS4, MARHS2 and
Iron acquisition and metabolismIron acquisition and metabolism - no subcategoryHeme, hemin uptake and utilization systems in GramPositives Uncharacterized siderophore biosynthesis protein near heme transporter HtsABC ...
Iron acquisition and metabolismIron acquisition and metabolism - no subcategoryHeme, hemin uptake and utilization systems in GramPositives Iron compound ABC uptake transporter permease protein ...
Therapeutic options for management of diabetic gastroparesis are limited. Failure to maintain upregulation of heme oxygenase 1 (HO1) leads to loss of interstitial cells of Cajal and delayed gastric emptying in diabetic non-obese diabetic mice.. HO1 is an enzyme which protects cells from physical, chemical, and biologic stress. In mice with diabetes and slow gastric emptying, hemin increases HO-1 activity and improves gastric emptying. Hemin is produced from red blood cells and is approved by the Food and Drug Administration for treating acute porphyria, which is an inherited condition caused by an enzyme deficiency. Hemin is not approved by the Food and Drug Administration for treating gastroparesis.. In this study subjects were randomized to intravenous hemin, prepared in albumin, or albumin alone. After infusions on days 1, 3, and 7, weekly infusions were administered for 7 weeks. Assessments included blood tests for HO1 protein and enzyme activity levels, gastric emptying with 13^C-spirulina ...
Neisseria gonorrhoeae, the etiologic agent of gonorrhea, utilizes TonB-dependent transporters to import essential nutrients such as iron. Study of TonB-dependent transporters is extremely important due to the fact that they make excellent vaccine targets. In order to learn more about the structure, function, expression, and regulation of selected TonB-dependent transporters, three goals were established for this study. The first goal was to examine the role of two highly conserved regions of TbpB in lipidation. One of the conserved regions of TbpB, the LSAC motif, was shown to be critical for lipidation. The second goal was to determine whether MisR/MisS regulates expression of TbpA and TbpB. MisR/MisS was shown to regulate the expression of TbpA and TbpB. The third goal was to assess the ability of recombinant TdfJ to bind hemin when expressed in E. coli. Recombinant TdfJ was shown to specifically bind hemin when expressed in E. coli.
Figure 2. Dependency of hemin catabolism on riboflavin bioavailability. The elimination of hemin requires cyclic reduction of heme oxygenase by flavoprotein cytochrome P450 reductase that, in turn, utilizes both flavin mononucleotide (FMN) and flavin-adenine dinucleotide (FAD) as prosthetic groups. Average or increased red meat consumption may overload the capacity of this chain of reactions already compromised by impaired intestinal absorption of riboflavin (with decreased FMN and FAD synthesis), leading to increased hemin (iron) delivery to the CNS and increased utilization of riboflavin for hemin inactivation. Modified from Figure 1, box 21-1, page 783 of Ref. 2 ...
The implications of this research, identify hemin as a potential therapeutic target for sporadic Creutzfeldt-Jakob disease (CJD) and cerebral hemorrhage.
1. Pyridine reacts with alkaline haematin to form a dipyridine dihydroxy dimeric haematin in which there is no competition between pyridine and OH− for coordination positions on the iron of haematin. 2. Imidazole competes directly with OH− to form the monomeric imidazole parahaematin in alkaline media. 3. The monomeric imidazole parahaematin aggregates readily to form a species that is precipitated on standing. 4. A structure is proposed for the haematin-pyridine compound in alkaline media.. ...
Comments to the paper "Observations on the Alkaline Haematin/Detergent Complex for Measuring Haemoglobin Concentration by O. W. van Assendelft and W. G. Zijlstra (1 ...
Lee, Heung-Man; Park, Il-Ho; Shin, Jae-Min; Yoon, Hyun-Sun; Park, Gyeong Yul; Zeher, Margit; Matsui, Katsuhiko; Tamai, Saki; Ikeda, Reiko; Suri, Drsushil; Suri, Dranu; Arani, Marzieh Heidarzadeh; Lubis, Azwin; Endaryanto, Anang; Koga, Shinichiro; Suk, Lee Ju; Tsuzuki, Yasunobu; Kim, Seo Hyeong; Shin, Jung U.; Noh, Ji Yeon; Jin, Shan; Jin, Shan; Lee, Hemin; Lee, Jungsoo; Park, Chang Ook; Lee, Kwang Hoon; Lee, Kwang Hoon; Tepetam, Fatma Merve; Park, Chun Wook; Son, Jee Hee; Cho, Soo Ick; Cho, Yong Se; Byun, Yun Sun; Yang, Yoon Seok; Chung, Bo Young; Kim, Hye One; Cho, Hee Jin; Katada, Yoshinori; Tanaka, Toshio; Nakabayashi, Akihiko; Nishida, Koji; Aoyagi, Kenichi; Tsukamoto, Yuki; Konma, Kazushi; Matsuura, Motoo; Park, Jung-Won; Harada, Yoshinori; Jeong, Kyoung Yong; Yura, Akiko; Yoshimura, Maiko; Kyung, Tae-Suk; Kim, Young Hyo; Park, Chang-Shin; Jang, Tae Young; Heo, Min-Jeong; Jung, Ah-Yeoun; Yang, Seung-Chan; Kim, Hye One; Cho, Yong Se; Byun, Yun Sun; Yang, Yoon Seok; Chung, Bo Young; Son, Jee ...
Purpose: Diabetic retinopathy (DR) is a progressive neurodegenerative disease and a leading cause of blindness. Overexpression of Heme Oxygenase-1(HO-1) by hemin induction protected retinal ganglion cells in diabetic retinopathy through anti-inflammatory, anti-apoptotic, and anti-proliferative effects. We investigated microRNA (miRNA) alterations in DR after hemin treatment with specific focus on miR-126, and its downstream target, HO-1.. Methods: miRNA expression profiling microarray (Affymetrix MicroRNA 2.0 Array) was used to examine the retinas of treated and non-treated streptozotocin- induced diabetic rats. Expressions of specific miRNAs were verified with PCR in the rat retina and in glucose-exposed endothelial cells. A target search, based on sequence complementarities, identified specific targets. We analyzed mRNA levels and protein expression in endothelial cells from large vessels and retinal capillaries and in the rat retina, with or without injection of miR-126 mimic or antagomir. ...
Post-liver transplantation acute lung injury (ALI) severely affects patients survival, whereas the mechanism is unclear and effective therapy is lacking. The authors postulated that reperfusion-induced increased oxidative stress plays a critical role in mediating post-liver transplantation ALI and that induction of heme oxgenase-1 (HO-1), an enzyme with anti-oxidative stress properties, can confer effective protection of lung against ALI. Male Sprague-Dawley rats underwent autologous orthotopic liver transplantation (OALT) in the absence or presence of treatments with the selective HO-1 inducer (Hemin) or HO-1 inhibitor (ZnPP). Lung tissues were collected at 8 h after OALT, pathological scores and lung water content were evaluated; survival rate of rats was analyzed; protein expression of HO-1 was determined by western blotting, and nuclear translocation of Nuclear factor erythroid 2-related factor 2 (Nrf2) and nuclear factor(NF)-κB p65 were detected by Immunofluorescence staining. The inflammatory
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Cairan empedu berasal dari penghancuran hemoglobin dari eritrosit yang telah tua. Hemoglobin ini akan di uraikan menjadi hemin, zat besi, dan globin. Zat besi dan globin akan di simpan di dalam hati kemudian di kirim ke sum-sum tulang merah. Zat-zat tersebut digunakan dalam pembentukan antibodi atau hemoglobin baru. Sementara itu, Hemin akan di rombak menjadi bilirubin dan biliverdin. Bilirubin dan biliverdin ini merupakan zat warna bagi empedu dan mengandung warna hijau-biru. Zat warna tersebut di dalam usus akan mengalami oksidasi menjadi urobilin. Urobilin kemudian di eksresikan dari dalam tubuh dan memberi warna kekuningan pada feses dan urine ...
4JES: The Hemophore HasA from Yersinia pestis (HasAyp) Coordinates Hemin with a Single Residue, Tyr75, and with Minimal Conformational Change.
Hemolytic anemia is one of the hallmarks of malaria and leads to an increase in oxidized heme (hemin) within the plasma of infected individuals. While scavenger proteins sequester much of the circulating heme, it has been hypothesized that extracellular heme may play a central role in malaria pathogenesis. W CSC100: Celebrating Canadian Chemistry
The preparation from ferriprotoporphyrin IX (FP) in aqueous acid medium of the related pigments beta- and B-hematin [see G. Blauer and M. Akkawi, Biochem. Mol. Biol. Int. 35, 231 (1995)] is presented under different conditions. Both pigments are characterized by infrared spectra which differ in the range of 1600-1700 cm-1 in their strong bands with absorption peaks measured at 1648 +/- 2 cm-1 for B-hematin and at 1663 +/- 1 cm-1 for beta-hematin. The pH dependence of B-hematin formation at 37 degrees C and at different concentrations of acetic acid and FP exhibits a maximum yield near pH 4. The formation of beta-hematin at 70 degrees C shows high yield in 6 M acetic acid or in the presence of 0.028 M trichloroacetate at pH 4.6. The dependence of the yield of the pigments on the time and temperature of incubation, concentration of FP, and the presence of different electrolytes was investigated. Both B- and beta-hematin are either insoluble or very slightly soluble in different solvents at room
1.3 mg/day. Therefore, if the PD patients had a normal absorptive capacity for vitamin B2, their large ingestion of red meat (up to 700 g/day), associated with milk, rice and beans, fruits and vegetables, should have provided a normal riboflavin status. In contrast, 31 consecutive PD patients had laboratory evidence for riboflavin deficiency (Table 2) suggesting that patients with sporadic PD belong to the subset of the general population (10-15%) (3) that may express a flavokinase with low affinity for vitamin B2, leading to a decreased absorption.. However, the digestion of red meat releases hemin, a highly diffusible toxin that, when not properly inactivated, increases intracellular iron concentrations and enhances hydroxyl radical production (Fenton reaction). Most of the absorbed hemin is destroyed by the enzyme heme oxygenase (HO) in the digestive tract and liver (26). Because HO is oxidized during the catabolization of hemin to biliverdin, the HO molecules must be reduced through the ...
The role of heme in the regulation of δ-aminolevulinic acid (ALA) synthetase was studied in fetal rat liver. The activity of ALA synthetase, the first and rate-limiting enzyme in the heme-biosynthetic pathway, is 10 times higher in fetal rat liver mitochondria than in adults. Hemin injection depresses mitochondrial ALA synthetase activity in the adult but not in the fetus. Cycloheximide, a selective inhibitor of protein synthesis in cytoplasmic ribosomes, but not in mitochondria, causes a rapid decrease in fetal mitochondrial ALA synthetase activity. When hemin is given prior to cycloheximide, a slower rate of turnover of mitochondrial ALA synthetase activity occurs than after cycloheximide alone. Treatment with 3-amino-1,2,4-triazole, an inhibitor of δ-aminolevulinic acid dehydratase, the second enzyme in heme biosynthesis, causes a decrease in fetal mitochondrial ALA synthetase activity but no decrease in the extramitochondrial enzyme levels. These studies indicate that heme may facilitate ...
1. Wheatgrass Juice is one of the best sources of living chlorophyll available today. However, to get the full benefit, the chlorophyll must come fresh from a living plant.. 2. Wheatgrass juice contains up to 70% chlorophyll, which is an important blood builder. The chlorophyll molecules closely resemble that of the hemin molecule, the pigment which combines with protein to form hemoglobin. The major difference is the chlorophyll molecule contains magnesium as its central atom, and the hemin molecule contains iron. The molecular structure of these two substances is almost identical in all other respects.. 3. Chlorophyll is the molecule that absorbs sunlight and uses its energy to synthesize carbohydrates from CO2 and water. This process is known as photosynthesis, a complex biochemical pathway in which solar energy is used to convert water and carbon dioxide to glucose and other carbohydrates, and is the basis for sustaining the life processes of all plants. Since animals and most humans obtain ...
If you have a question about this talk, please contact Frida Kaori Weierud.. Abstract not available. This talk is part of the Foster Talks series.. ...
may be the causative agent from the biphasic human being disease Oroya fever. Oxidative tension (1 mM H2O2) and hemin restriction got no significant influence SRSF2 on mRNA amounts. Dedication of proteins quantities using immunoblotting and SDS-PAGE showed the best levels of under acidic circumstances or in 20 °C. The least quantity of was synthesized […]. ...
Heme oxygenase-1 (HO-1), an antioxidant defense enzyme, has been shown to protect against oxidant-induced liver injury. However, its role on liver fibrosis remains unclear. This study aims to elucidate the effect and the mechanism of HO-1 in nutritional fibrosing steatohepatitis in mice. Male C57BL/6J mice were fed with a methionine-choline deficient (MCD) diet for eight weeks to induce hepatic fibrosis. HO-1 chemical inducer (hemin), HO-1 chemical inhibitor zinc protoporphyrin IX (ZnPP-IX) and/or adenovirus carrying HO-1 gene (Ad-HO-1) were administered to mice, respectively. Liver injury was assessed by serum ALT, AST levels and histological examination; hepatic lipid peroxides levels were determined; the expression levels of several fibrogenic related genes were assayed by real-time quantitative PCR and Western blot. MCD feeding mice showed progressive hepatic injury including hepatic steatosis, inflammatory infiltration and fibrosis. Induction of HO-1 by hemin or Ad-HO-1 significantly attenuated the
INTRODUCTION: Remifentanil preconditioning (RPC) can protect the heart from ischemia-reperfusion injury (IRI). Heme oxygenase-1 (HO-1) can also reduce the cardiac infarct size during ischemia reperfusion in vivo. Our aim was to investigate whether HO-1 expression participates in RPC cardio protection. METHODS: IRI was induced in male Sprague-Dawley rats (300g) by occluding the left coronary artery for 30 minutes followed by 2 hours of reperfusion. 6μg/kg/min remifentanil as administered in three infusion cycles of 5-min (RPC group). Hemin, an agonist of HO-1, 30mg/kg/d was given intraperitoneally for 2 days before RPC (RPC+hemin group). Tin Protoporphyrin IX (SnPP), which can inhibit HO-1 expression, was injected 0.7 mg/kg/day subcutaneously for 2 days before RPC (RPC+SnPP group). The non-operation group was marked as sham. In the control group, 0.9% saline replaced remifentanil. After the operation, the heart infarct size was determined by 2, 3, 5-triphenyltetrazolium staining. Expression of ...
target_id = "014432" $split = "___" $target = array( "Target" => array( "id" => "14861", "target_id" => "014432", "local_target_id" => "014432", "locus_tag" => "RHE_CH03268", "ncbi_gi" => "86282969", "ncbi_accession" => "ABC92032.1", "ncbi_geneid" => null, "ncbi_taxon_id" => "347834", "gene_name" => "hmuS", "common_name" => "hemin transport system, degrading protein [Rhizobium etli CFN 42]", "ncbi_coded_by_region" => "CP000133.1:3430983..3432032", "ncbi_note" => "similar to HmuS [Rhizobium leguminosarum] and hmuS (SMc01513) [Sinorhizobium meliloti]; Similar to entrez-protein:CAC34392.1; Putative location:bacterial cytoplasm Psort-Score: 0.4277", "tigr_main_role_id" => null, "tigr_sub_role_id" => null, "comment" => null, "justification" => "Symbiotic and global nitrogen fixation based on ortholog expansion from Sinorhizobium", "sequence" => ...
Dashper, S. G. et al "Characterization of a Novel Outer Membrane Hemin-Binding Protein of Porphyromonas gingivalis ." Journal of Bacteriology 182.22 (2000): 6456-6462. Web. 20 Nov. 2019. ...
There is no effective drug for the therapy of acute carbon monoxide (CO) poisoning. The purpose of the present study was to investigate the potential preventive and therapeutic effects of hemin on an animal model of acute CO poisoning and to provide a potential therapeutic candidate drug. A total of 80 Kunming mice were randomly divided into four groups, namely the air control, acute CO poisoning, hemin-treatment + CO and hemin-pretreatment + CO groups (n=20 each). Furthermore, the mortality rate of mice, blood carboxyhaemoglobin (HbCO) concentration and serum malondialdehyde (MDA) concentration were measured, and pathological changes of the hippocampal area were determined using histochemical staining ...
Additional comment: After the equilibration, the IPG gel strips were cut to size. Six mm were removed from the anodic end and 14 mm from the cathodic end. The second dimension gels were overlayered with a solution containing agarose (0.5% w/v) and Tris-glycine-SDS (25 mM-198 mM-0.1% w/v) pH 8.3 heated at about 70o C and the IPG gel strips were immediately loaded through it ...
There are 11 seven-letter words containing A, H, M, N and T: ANTHEMS HEMATIN HETMANS ... PHANTOM THIAMIN XANTHAM. Every word on this site can be played in scrabble. Create other lists, that begin with or end with letters of your choice.
Talk Title: Pulse Azidohomoalanine (AHA) Labeling in Mammals (PALM) Analysis for Global Analysis of Newly-synthesized Proteins in Animal Models of Disease. VIEW MORE. ...
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Differentiation in murine erythroleukemic cells is arrested at the proerythroblast stage. A small fraction of the population, however, undergoes spontaneous differentiation. This spontaneous differentiation was examined at the individual cell level in relation to cell multiplication, commitment, and maturation. The results indicate that murine erythroleukemic cells destined to undergo spontaneous differentiation first undergo commitment, an irreversible process characterized by cells becoming (a) capable of producing hemoglobin coupled with (b) a loss of their ability to undergo more than six subsequent cell divisions. Commitment is followed by a maturation process which includes the accumulation of erythroid specific markers, e.g., hemoglobin. With respect to commitment, spontaneous differentiation resembles the differentiation produced by most inducers but differs from that evoked by hemin. Serum hemin may therefore be exempt from implication in the spontaneous process. ...
Background: Dental technicians (DTs) are exposed to Beryllium (Be) and other substances capable of inducing lung disease. Heme oxygenase -1 (HO-1) play a protective antioxidant role in the lung. The guanine-thymidine (GT) n repeats in the HO-1 promoter determine HO-1 induction level. Short (GT) n repeats (n = ,25; S genotype) is considered as protective since HO-1 is induced more rapidly than in long (GT) n repeats (n = ≥25; L genotype).. Aims To evaluate the correlation of HO-1 polymorphisms to functional and exposure parameters in DTs and the protective role of HO-1 on Be Oxide (BeO) exposed A549 epithelial cells apoptosis.. Methods 65 DTs were followed-up for 2 years by questionnaires,induced sputum (IS) particles size distribution laser analysis (Dapi 2000 Donner Tech and Pulmonary Function Tests. HO-1 genotyping was done by PCR DNA sequencer (AB prism 310). A549 epithelial cell line was cultured with BeO and pretreated with Hemin and Znpp (for stimulation and inhibition of HO-1 ...
Good girl.... Gone are the days when I had 5 litre flasks of Harriss haematoxylin ripening in the Stoke-on-Trent sunshine; I felt like an alchemist. Then it was plagiarised Gills haematoxylin ripening, then some infernal lead haematoxylin concoction that turned everything it touched black; I strangely put on weight too. The ripening of haematoxylin to haematin then to oxyhaematin was a delight to witness, the latter meant you have gone too far. The ability to carry out an H&E progressively without having to remove the cytoplasmic overstaining a delight. Im one for keeping home made haematoxylin together with retaining the a in haematin, but on both points alas I feel I shall fail. -----Original Message----- From: Bauer, Karen [mailto:[email protected]] Sent: 01 March 2005 18:02 To: [email protected] Subject: [Histonet] Hematoxylin[Scanned] Hello, I was just wondering what everyone is using for Hematoxylin these days. We are one of those rare Histology Labs that makes ...
Activation of heme oxygenase-1 (HO-1), a heme-degrading enzyme responsive to a wide range of cellular stress, is traditionally considered to convey adaptive responses to oxidative stress, inflammation and vasoconstriction. These diversified effects a
TY - JOUR. T1 - Heme arginate and the endothelium. T2 - mechanism for its safety in porphyria. AU - Balla, J.. AU - Balla, G.. AU - Kakuk, G.. AU - Nath, K. A.. AU - Jacob, H. S.. AU - Vercellolti, G. M.. PY - 1996. Y1 - 1996. N2 - Acute intermittent porphyria (AIP) is a potential fatal disease characterized by decreased synthesis of heme and accumulation of porphyrin precursors. Infusion of hematin has proven efficacious, but with considerable vascular side effects such as thrombosis and DIG; in contrast, Finnish investigators have demonstrated that heme arginate (HA) is both effective and non-vasculotoxic. We have previously shown that heme (hemin chloride) serves as a catalytically active iron source, potentiating the oxidation of LDL and sensitizing endotnelial cells (EC) to oxidant injury. EC respond to heme and oxidant challenge by upregulating the cell cytoprotectants, heme oxygenase (HO) and ferritin. We now provide a mechanism that accounts for the relative safety and efficacy exhibited ...
In situ surface-enhanced Raman scattering studies of the nitrosyl adduct of hemin adsorbed roughened silver surfaces in aqueous electrolytes
TY - JOUR. T1 - HO-1 Overexpression Attenuates Endotoxin Effects on CAT-2 Isozymes Expression. AU - Huang, Te Yang. AU - Tsai, Pei Shan. AU - Huang, Chun Jen. PY - 2008/8. Y1 - 2008/8. N2 - Background: l-arginine transport mediated by type-2 cationic amino acid transporter (CAT-2) isozymes is one crucial mechanism that regulates nitric oxide (NO) production via inducible nitric oxide synthase (iNOS). We sought to investigate the effects of heme oxygenase-1 (HO-1) overexpression on CAT-2 isozymes, e.g., CAT-2, CAT-2A, and CAT-2B. Materials and Methods: Adult male Sprague Dawley rats were allocated to receive lipopolysaccharide (LPS), normal saline, hemin (a HO-1 inducer), tin protoporphyrin (SnPP, a HO-1 inhibitor), LPS plus hemin, or LPS plus hemin plus SnPP. After maintaining for 6 h, rats were sacrificed and the expression and activity of individual enzyme was evaluated. Results: LPS increased HO activity, HO-1 concentration, NO production, l-arginine transport, and concentrations of iNOS, ...
pentaerythritol tetranitrate reductase: catalyzes denitration of pentaerythritol tetranitrate with NADPH to 3-hydroxy-2,2-bis-((nitroxy)methyl)propanal and 2,2-bis-((nitroxy)methyl)-propanedial; isolated from Enterobacter cloacae
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Hans Fischer (27 July 1881 - 31 March 1945) was a German organic chemist and the recipient of the 1930 Nobel Prize for Chemistry "for his researches into the constitution of haemin and chlorophyll and especially for his synthesis of haemin." Fischer was born in Höchst on Main, now a city district of Frankfurt. His parents were Dr. Eugen Fischer, Director of the firm of Kalle & Co, Wiesbaden, and Privatdozent at the Technical High School, Stuttgart, and Anna Herdegen. He went to a primary school in Stuttgart, and later to the "Humanistisches Gymnasium" in Wiesbaden, matriculating in 1899. He read chemistry and medicine, first at the University of Lausanne and then at Marburg. He graduated in 1904, and in 1908 he qualified for his M.D..[citation needed] He worked first at a Medical Clinic in Munich and then at the First Berlin Chemical Institute under Emil Fischer. He returned to Munich in 1911 and qualified as lecturer on internal medicine one year later. In 1913 he became a lecturer in ...
Very little is known about heme transport in eukaryotes and a heme-specific transport uptake systems have not been identified in yeast. Heme is synthesized in m...