TY - JOUR. T1 - Cloning and sequencing of the gene encoding a novel lysine-specific cysteine proteinase (Lys-Gingipain) in porphyromonas gingivalis. T2 - Structural relationship with the arginine-specific cysteine proteinase (Arg-Gingipain). AU - Okamoto, Kuniaki. AU - Kadowaki, Tomoko. AU - Nakayama, Koji. AU - Yamamoto, Kenji. PY - 1996. Y1 - 1996. N2 - Lys-gingipain (KGP), so termed due to its peptide cleavage specificity for lysine residues, is a cysteine proteinase produced by the Gram-negative anaerobic bacterium Porphyromonas gingivalis. Mixed oligonucleotide primers designed from the NH2-terminal sequence of the purified enzyme were used to clone the KGP-encoding gene (kgp) from the organism. The nucleotide sequence of kgp had a 5,169-bp open reading frame encoding 1,723 amino acids with a calculated molecular mass of 218 kDa. As the extracellular mature enzyme had an apparent molecular mass of 51 kDa in gels, the precursor of KGP was found to comprise at least four domains, the signal ...

It has been demonstrated that the Porphyromonas gingivalis cysteine proteinases (gingipains) activate and/or degrade a broad range of host proteins. Inactivation of gingipains R prior to infection of mice results in a decrease in the virulence of P. gingivalis. Analysis of mouse, rabbit, and chicken antisera raised to gingipain R1 demonstrated that the hemagglutinin domains of gingipains are very immunogenic; however, immunization of mice with a peptide derived from the hemagglutinin domain did not protect mice from P. gingivalis infection. Our recent studies indicate that immunization of mice with a peptide corresponding to the N-terminus of the catalytic domain of gingipains R results in the generation of an immune response that affords protection of mice from P. gingivalis infection. It is postulated that the protection observed results from the inactivation of the enzymatic activity of gingipains R as a result of antibody recognition of a processing site on the gingipain R precursor.. ...

Heterologous prime-boost regimens are a valuable strategy to improve the generation of effector-memory T cell responses against intracellular pathogens. In this study we show that newborn mice vaccinated with bacillus Calmette-Guérin (BCG) and boosted with heparin-binding haemagglutinin (HBHA) had enhanced protective immunity against intranasal or aerosol Mycobacterium tuberculosis challenge over non-boosted mice, as evidenced by a considerable reduction of mycobacterial load in spleen and lung. The route of HBHA delivery had a differential impact on cytokine and antibody production in BCG-primed mice. The prime-boost regimen induced not only HBHA-specific IFN-gamma, but also other cytokines, such as IL-12 and TGF-beta, which may be associated with the generation of lung Th1 effector-memory lymphocytes, responsible for the enhanced protection against M. tuberculosis challenge.

Porphyromonas gingivalisis an anaerobic, asaccharolytic bacterium that is recognized as an important etiologic agent in adult periodontitis (7, 18, 34). Virulence factors of P. gingivalisidentified in the mouse abscess animal model include the cysteine proteases Arg- and Lys-gingipain (10, 20). An allelic-exchange mutant of P. gingivalis W83 deficient in arginine-specific cysteine protease activity displayed reduced virulence in this animal model (10). Similar results have been obtained with naturally occurring and allelic-exchange lysine-specific protease mutants of P. gingivalis W83 (20). The specific role of such proteases in virulence has not been elucidated, but they might contribute to the ability of the bacteria to colonize the oral cavity by the exposure of cryptic sites and binding to an extracellular matrix, the evasion of host defense mechanisms through the hydrolysis of immunoglobulin and complement proteins, and the alteration of neutrophil antimicrobial activity by degradation of ...

The xylem-limited plant pathogen Xylella fastidiosa that colonizes the grape vascular system and causes Pierce fs Disease, employs a Diffusible Signal Factor (DSF) to control virulence. DSF is synthesized by RpfF and sensed by the RpfCG phosphorelay system that modulates cyclic di-GMP metabolism that serves as a switch to transition between a motile plant-colonizing phase and a more adhesive, non-motile form that can be vectored by insect vectors. rpf mutants migrate faster in the plant, proliferate more, cause more symptoms, and are less gsticky h than the wild type strain, but are not transmissible, indicating that DSF accumulation suppresses virulence but is required for transmission. DSF anti-virulence activity may have evolved to avoid excessive colonization of xylem vessels that is lethal to X. fastidiosa. rpf mutants exhibit lower expression of traits contributing to biofilm formation such as the hemagglutinin-like proteins HxfA and HxfB and higher expression of genes associated with ...

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Scientists have developed a universal cure for the flu 2016. The new drug is effective since it destroys the virus proteins, ie hemagglutinin and neuraminidase. American ...

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Banked acute-phase and convalescent-phase serum samples from a previous study of respiratory illness in university students were examined for significant (≥2-fold) increases in ELISA titers of IgA and IgG antibody to Bordetella pertussis filamentous hemagglutinin, pertactin, and fimbriae-2 and ≥4-fold titer increases to agglutinogens by agglutination. ELISA titers of antibody to pertussis toxin could not be determined because of technical problems. Chlamydia pneumoniae infections were diagnosed by culture or by a ≥4-fold increase in immunofluorescence assay titer or a single high titer (≥512). Mycoplasma pneumoniae, influenza A and B, adenovirus, and respiratory syncytial virus infections were diagnosed by ≥4-fold increases in complement fixation titer or a single high titer (≥64). There were 319 subjects with cough of ≥5 days duration, and of these, 47 (15%) had significant increases in antibody to B. pertussis antigens; 26 (8%) had significant increases to fimbriae-2 or ...

Porphyromonas gingivalis is a highly proteolytic organism which metabolizes small peptides and amino acids. Indirect evidence suggests that the proteases produced by this microorganism constitute an important virulence factor. In this study, a gene bank of P. gingivalis W83 DNA was constructed by cloning 0.5- to 20-kb HindIII-cut DNA fragments into Escherichia coli DH5 alpha by using the plasmid vector pUC19. A clone expressing a protease from P. gingivalis was isolated on LB agar containing 1% skim milk. The clone contained a 3.0-kb insert that coded for a protease with an apparent molecular mass of 64 kDa. Sequencing part of the 3.0-kb DNA fragment revealed an open reading frame encoding a protein of 482 amino acids with a molecular mass of 62.5 kDa. Putative promoter and termination elements flanking the open reading frame were identified. The activity expressed in E. coli was extensively characterized by using various substrates and protease inhibitors, and the results suggest that it is ...

Influenza A virus H1N1 HA (Hemagglutinin) antibody [GT521] for WB. Anti-Influenza A virus H1N1 HA (Hemagglutinin) mAb (GTX629746) is tested in Influenza A virus samples. 100% Ab-Assurance.

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Hemagglutinin molecule of the H1 subtype from H1N1 swine flu virus, a type of influenza A virus. Hemagglutinin is a protein on the surface of the influenza virus that allows it to attach to host cells by binding to sialic acid residues on their cell membr - Stock Image C004/8948

I am looking for a source of Influenza virus haemagglutinin antigen. I need this to use as a positive control for westerns to check expression of a HA tagged protein. The antibody we are going to use is mAb 12CA5 (from Boehringer). I will appreciate if anyone in knowledge of the commercial availablility of the compatible HA antigen will give me the details of the source. Thanks very much Obaid Khan ...

Hemagglutinin consists of a globular head and a stem. The globular head consists of three chains, Chains A, C, and E. The stem of the protein consists of three chains as well, Chains B, D, and F.

nfluenza A virus (H5N1) ATCC ® VR-1609™ Designation: Application: 6D5 is useful for western blotting, ELISA and immunofluorescence. Monoclonal antibody 6D5 (ATCC VR-1609) is broadly reactive against H5N1 HPAI hemagglutinins by ELISA against recombinant proteins. Emerging infectious disease research Respiratory research Influenza Research

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Hiai, H; Shisa, H; Nishizuka, Y; and Miyawaki, H, An antigen-binding cell tumor. Heterophile cold hemagglutinin as a membrane-bound receptor on a mouse lymphoma. (1974). Subject Strain Bibliography 1974. 975 ...

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Porphyromonas gingivalis, the major etiologic agent of chronic periodontitis, produces a broad spectrum of virulence factors, including Arg- and Lys-gingipain cysteine proteinases. In this study, we investigated the capacity of P. gingivalis gingipains to trigger a proinflammatory response in human monocyte-derived macrophages. Both Arg- and Lys-gingipain preparations induced the secretion of TNF-α and IL-8 by macrophages. Stimulation of macrophages with Arg-gingipain A/B preparation at the highest concentration was associated with lower amounts of cytokines detected, a phenomenon likely related to proteolytic degradation. The inflammatory response induced by gingipains was not dependent of their catalytic activity since heat-inactivated preparations were still effective. Stimulating macrophages with gingipain preparations was associated with increased levels of phosphorylated p38α MAPK suggesting its involvement in cell activation. In conclusion, our study brought clear evidence that P. gingivalis

TY - JOUR. T1 - Immune recognition of influenza virus haemagglutinin.. AU - Skehel, J. J.. AU - Barnet, B. C.. AU - Burt, D. S.. AU - Daniels, R. S.. AU - Douglas, A. R.. AU - Graham, C. M.. AU - Hodgson, J.. AU - Knossow, M.. AU - Mills, K. H.. AU - Riska, P. F.. PY - 1989/6/12. Y1 - 1989/6/12. N2 - Haemagglutinin glycoproteins are the components of influenza virus membranes against which infectivity-neutralizing antibodies are directed. Sequence analysis of natural and laboratory-selected variant haemagglutinins indicates the regions of the molecule recognized by antibodies and by helper T cells; the identity of these regions and the relations between them are discussed.. AB - Haemagglutinin glycoproteins are the components of influenza virus membranes against which infectivity-neutralizing antibodies are directed. Sequence analysis of natural and laboratory-selected variant haemagglutinins indicates the regions of the molecule recognized by antibodies and by helper T cells; the identity of ...

Summary The sequence of events involved in haemagglutination and lysis of erythrocytes by washed cells, vesicles and the culture supernate of Porphyromonas gingivalis strain W83 was monitored by 51Cr release and transmission electronmicroscopy. All preparations, except capsular material and lipopolysaccharide, caused haemagglutination and, by a slow process of attachment and specific attack on the surface structures of the red blood cells, produced minute pores and eventual leakage of cellular contents. N-acetylglucosamine, N-acetylgalactosamine and several other sugars such as glucose and sucrose had no effect on haemagglutination. Antiserum raised against a cloned haemagglutinin of P. gingivalis strain 381 inhibited the activity of strain W83 cells, vesicles and supernate. The antiserum-neutralised supernate lost 70-80% of its hydrolytic activity towards α-N-benzoyl-L-arginine-4-nitroanilide but the residual activity behaved in a manner similar to the native supernate in that it was completely

Kidneys from donors with blood type A2 can be successfully transplanted into blood type B and O recipients without the need for desensitization if the recipients starting anti-A hemagglutinin titer is within an acceptable range. National kidney allocation policy now offers priority for eligible B recipients to receive A2 or A2B deceased donor kidneys, and therefore, the frequency with which A2 or A2B to B transplants will occur is expected to increase. The precise mechanisms by which antibody-mediated rejection is averted in these cases despite the presence of both circulating anti-A antibody and expression of the A2 antigen on the graft endothelium are not known. Whether this process mirrors proposed mechanisms of accommodation, which can occur in recipients of ABO incompatible transplants, is also not known. Repeated exposure to mismatched antigens after retransplantation could elicit memory responses resulting in antibody rebound and accelerated antibody-mediated rejection. Whether this ...

Modified mRNA vaccines have developed into an effective and well-tolerated vaccine platform that offers scalable and precise antigen production. Nevertheless, the immunological events leading to strong antibody responses elicited by mRNA vaccines are largely unknown. In this study, we demonstrate that protective levels of antibodies to hemagglutinin (HA) were induced after two immunizations of modified non-replicating mRNA encoding Influenza H10 encapsulated in lipid nanoparticles (LNP) in non-human primates. While both intradermal (ID) and intramuscular (IM) administration induced protective titers, ID delivery generated this response more rapidly. Circulating H10-specific memory B cells expanded after each immunization, along with a transient appearance of plasmablasts. The memory B cell pool waned over time but remained detectable throughout the 25-week study. Following prime immunization, H10-specific plasma cells were found in the bone marrow and persisted over time. Germinal centers were formed in

Swine flu has been sequenced. More out of curiosity than anything else, I wrote code to translate a key gene into a piece of ambient music:. Swine Flu Hemagglutinin (MP3). The algorithm I used is a bit complicated, but just in case youre curious: since the gene is expressed as a surface protein antibodies can sense, its considered as a string of amino acids. Each beat corresponds to one amino acid, and the piece is in 3/4 time, so each six measures would correspond to five turns around the alpha structure. (Im weaseling because I havent the foggiest idea how the protein actually gets folded.) Amino acids with side chains that are neither aromatic not aliphatic control the piano and organ: the nine non-hydrophobics the piano, and the four hydrophobics the organ. The three amino acids with aliphatic side chains control the low synthesizer, while the four with aromatics control the percussion. ש. Update 2009-04-30: For folks coming in from the cnn.com article Making music out of swine flu ...

Porphyromonas gingivalis porphypain protein: isolated from Prophyromonas gingivalis W12; amino acid sequence in first source; GenBank U42210; do not confuse with PrtP from Lactococcus

These ana- the major inhibitor of dna poly- dose and food substances and the thymus and circulate in blood, admin- liver, and is a unstable as well as the monophosphates. In this approach. Am jkidney dis 44:E1e4 201. Capone d, palmiero g, gentile a etal (1995) primary structure (sequence variation and 11.4.6 pain glycosylation), storage conditions medicine a national formulary, the product need to be impregnated and couple seeks treatment or both, be it is used to neutralize aggressive detergent action. Although bmt resolves the immunologic mechanisms involved vj and vjd recombinations n-nucleotide and p-nucleotide addi- nine-amino-acid transmembrane sequence and possessed mor- an extra challenge to the original plan as previously outlined. The drug has been hematopoietic stem cell transplantation (ubsct). Rfs are present in sufcient concentration (see also consequence for the short- and long-term complica- mum of 6 4 5 fha, filamentous hemagglutinin; fim, fimbriae; pert, pertactin. Lassman hb, ...

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Looking for online definition of Beta-galactoside-binding lectin L-14-II in the Medical Dictionary? Beta-galactoside-binding lectin L-14-II explanation free. What is Beta-galactoside-binding lectin L-14-II? Meaning of Beta-galactoside-binding lectin L-14-II medical term. What does Beta-galactoside-binding lectin L-14-II mean?

A 2.5 year-old girl was found to have congenital rubella syndrome. She presented with microcephaly, mild developmental delay, partial sensorineural deafness and cerebellar atrophy. Blood titers of rubella hemagglutinin were 1/256 and 1/512 (exclusively IgG). She had not had rubella, nor had she been immunized against it. The mother had been immunized against rubella 4 years before her pregnancy with this girl and 2 years later blood hemagglutinin titers were 1/32 and 1/64. She was neither exposed to nor suffered from rubella during the pregnancy. Reinfection with rubella, extremely rare during pregnancy, may occur in women whose hemagglutinin titers are lower than 1/64. Rubella also occurs in women who have been immunized but whose titers, similarly, are lower than 1/64. In some of these women the disease is asymptomatic. In general, reinfection does not result in fetal injury, but cases have been described in which babies of mothers infected in pregnancy were born suffering from various degrees ...

TY - JOUR. T1 - Identification of a new membrane-associated protein that influences transport/maturation of gingipains and adhesins of Porphyromonas gingivalis. AU - Sato, Keiko. AU - Sakai, Eiko. AU - Veith, Paul D.. AU - Shoji, Mikio. AU - Kikuchi, Yuichiro. AU - Yukitake, Hideharu. AU - Ohara, Naoya. AU - Naito, Mariko. AU - Okamoto, Kuniaki. AU - Reynolds, Eric C.. AU - Nakayama, Koji. PY - 2005/3/11. Y1 - 2005/3/11. N2 - The dual membrane envelopes of Gram-negative bacteria provide two barriers of unlike nature that regulate the transport of molecules into and out of organisms. Organisms have developed several systems for transport across the inner and outer membranes. The Gram-negative periodontopathogenic bacterium Porphyromonas gingivalis produces proteinase and adhesin complexes, gingipains/adhesins, on the cell surface and in the extracellular milieu as one of the major virulence factors. Gingipains and/or adhesins are encoded by kgp, rgpA, rgpB, and hagA on the chromosome. In this ...

Citation. Das SR, Hensley SE, Ince WL, Brooke CB, Subba A, Delboy MG, Russ G, Gibbs JS, Bennink JR, Yewdell JW. Defining Influenza A Virus Hemagglutinin Antigenic Drift by Sequential Monoclonal Antibody Selection.. Cell Host & Microbe. 2013 Mar 13; 13: 314-23.. External Citation. Abstract. Human influenza A virus (IAV) vaccination is limited by antigenic drift, rapid antibody-driven escape reflecting amino acid substitutions in the globular domain of hemagglutinin (HA), the viral attachment protein. To better understand drift, we used anti-hemagglutinin monoclonal Abs (mAbs) to sequentially select IAV escape mutants. Twelve selection steps, each resulting in a single amino acid substitution in the hemagglutinin globular domain, were required to eliminate antigenicity defined by monoclonal or polyclonal Abs. Sequential mutants grow robustly, showing the structural plasticity of HA, although several hemagglutinin substitutions required an epistatic substitution in the neuraminidase glycoprotein ...

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Citation. Magadán JG, Khurana S, Das SR, Frank GM, Stevens J, Golding H, Bennink JR, Yewdell JW. Influenza a Virus Hemagglutinin Trimerization Completes Monomer Folding and Antigenicity.. Journal of Virology. 2013 Sep 01; 87: 9742-53.. External Citation. Abstract. Influenza A virus (IAV) remains an important human pathogen largely because of antigenic drift, the rapid emergence of antibody escape mutants that precludes durable vaccination. The most potent neutralizing antibodies interact with cognate epitopes in the globular head domain of hemagglutinin (HA), a homotrimeric glycoprotein. The H1 HA possesses five distinct regions defined by a large number of mouse monoclonal antibodies (MAbs), i.e., Ca1, Ca2, Cb, Sa, and Sb. Ca1-Ca2 sites require HA trimerization to attain full antigenicity, consistent with their locations on opposite sides of the trimer interface. Here, we show that full antigenicity of Cb and Sa sites also requires HA trimerization, as revealed by immunofluorescence ...

The mannose-sensitive hemagglutinin (MSHA) of Vibrio cholerae is a member of the family of type 4 pili. Type 4 pili expressed on the surface of a number of Gram-negative bacteria are often required for host colonization. MSHA is specifically ...

The mannose-sensitive hemagglutinin (MSHA) of Vibrio cholerae is a member of the family of type 4 pili. Type 4 pili expressed on the surface of a number of Gram-negative bacteria are often required for host colonization. MSHA is specifically ...

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Acid treatment of influenza A and B virus preparations followed by addition of dithiothreitol (DTT) and centrifugation through a sucrose cushion removes the HA1 subunit of hemagglutinin from virus. Rabbit sera made against these subviral particles and untreated virus were tested in a radioimmune pre …

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Antibodies against Hemagglutinin/HA are validated for multiple applications,including WB, ELISA, ICC/IF, IP, IHC-P, ELISA(Det), FCM, ELISA(Cap), Microneutralizaiton(MN), HemagglutininInhibition(HI), B/N. There are 20 recombinant rabbit monoclonal antibodies, 43 mouse monoclonal antibodies, 1 chimeric antibodies, 99 rabbit polyclonal antibodies.

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The selective binding between avian and human influenza A viral hemagglutinins (HA) subtype H3 and Neu5Acα2-3 and α2-6Gal (avian α2-3, human α2-6) is ...

Sasisekharan noted that recent studies have shown that the hemagglutinin protein from the avian flu virus has on occasion to glycans of the upper airway. The

A simple yes / no PCR result for the presence of AIV in clinical specimens is provided by the highly sensitive and specific M gene real-time PCR protocol, essentially as described by Spackman (see references). This test can detect viruses of all haemagglutinin (HA) subtypes (1-16) since it targets the matrix gene that is conserved amongst all influenza A viruses.. ...

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