Playing Hide and Seek: How Glycosylation of the Influenza Virus Hemagglutinin Can Modulate the Immune Response to Infection. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
The A(H7N9) virus hemagglutinin protein has several motifs that are characteristic of mammalian-adapted and human influenza viruses, including mutations that confer human-type receptor-binding and enhanced virus replication in mammals. The pandemic risk rises exponentially should these viruses acquire the ability to transmit readily among humans.. Reports indicate that several A(H7N9) viruses from patients who were undergoing antiviral treatment acquired resistance to the primary medical countermeasure-neuraminidase inhibitors (such as oseltamivir, peramivir, and zanamivir). Acquisition of resistance to these inhibitors by A(H7N9) viruses could increase the risk of serious outcomes of A(H7N9) virus infections.. The hemagglutinin proteins of A(H7N9) viruses have a cleavage site consistent with a low-pathogenic phenotype in birds; in the past, highly pathogenic H7 variants (with basic amino acid insertions at the cleavage site that enable the spread of the virus to internal organs) have emerged ...
I am looking for a source of Influenza virus haemagglutinin antigen. I need this to use as a positive control for westerns to check expression of a HA tagged protein. The antibody we are going to use is mAb 12CA5 (from Boehringer). I will appreciate if anyone in knowledge of the commercial availablility of the compatible HA antigen will give me the details of the source. Thanks very much Obaid Khan ...
Biochemical tests used in the identification of infectious agents include the detection of metabolic or enzymatic products characteristic of a particular infectious agent. Since bacteria ferment carbohydrates in patterns characteristic of their genus and species, the detection of fermentation products is commonly used in bacterial identification. Acids, alcohols and gases are usually detected in these tests when bacteria are grown in selective liquid or solid media. The isolation of enzymes from infected tissue can also provide the basis of a biochemical diagnosis of an infectious disease. For example, humans can make neither RNA replicases nor reverse transcriptase, and the presence of these enzymes are characteristic of specific types of viral infections. The ability of the viral protein hemagglutinin to bind red blood cells together into a detectable matrix may also be characterized as a biochemical test for viral infection, although strictly speaking hemagglutinin is not an enzyme and has no ...
Hemagglutinin molecule of the H1 subtype from H1N1 swine flu virus, a type of influenza A virus. Hemagglutinin is a protein on the surface of the influenza virus that allows it to attach to host cells by binding to sialic acid residues on their cell membr - Stock Image C004/8948
Vaccine researchers have developed a strategy aimed at generating broadly cross-reactive antibodies against the influenza virus: embrace the unfamiliar.. In recent years, researchers interested in a "universal flu vaccine" identified a region of the viral hemagglutinin protein called the stem or stalk, which doesnt mutate and change as much as other regions and could be the basis for a vaccine that is protective against a variety of flu strains.. In an Emory Vaccine Center study, human volunteers immunized against the avian flu virus H5N1 readily developed antibodies against the stem region of the viral hemagglutinin protein. In contrast, those immunized with standard seasonal trivalent vaccines did not, instead developing most of their antibodies against the more variable head region. H5N1, regarded as a potential pandemic strain, is not currently circulating in the United States and the volunteers had not been exposed to it before.. The results were published Monday, August 25 in ...
Vaccine researchers have developed a strategy aimed at generating broadly cross-reactive antibodies against the influenza virus: embrace the unfamiliar.. In recent years, researchers interested in a "universal flu vaccine" identified a region of the viral hemagglutinin protein called the stem or stalk, which doesnt mutate and change as much as other regions and could be the basis for a vaccine that is protective against a variety of flu strains.. In an Emory Vaccine Center study, human volunteers immunized against the avian flu virus H5N1 readily developed antibodies against the stem region of the viral hemagglutinin protein. In contrast, those immunized with standard seasonal trivalent vaccines did not, instead developing most of their antibodies against the more variable head region. H5N1, regarded as a potential pandemic strain, is not currently circulating in the United States and the volunteers had not been exposed to it before.. The results were published Monday, August 25 in ...
Anti-Hemagglutinin/HA Antibody11713-T54, manufactured by Sino Biological is validated in WB,ELISA,FCM,ICC/IF,IP. Custom antibody services and bulk production also available. To learn more comprehensive our antibody product information including immunogen, specificity, and more, you can read all details here.
Anti-Hemagglutinin/HA Antibody11056-R014, manufactured by Sino Biological is validated in ELISA(Cap). Custom antibody services and bulk production also available. To learn more comprehensive our antibody product information including immunogen, specificity, and more, you can read all details here.
Experimentally induced adaptations in the 2009 H1N1 influenza virus led to dramatically increased replication rates and decreased receptor-binding affinity in human lung cells, according to findings published this week in Virology.. A team of German and Austrian researchers serially passaged a 2009 H1N1 variant isolate in human A549 lung epithelial cells to determine factors that might affect the strains future virulence.. After six passages, the viruss replication rate abruptly rose, causing a 100-fold increase in viral titers. Both the original isolate and the adapted strain infected A549 cells with similar effectiveness.. Investigators identified five mutations that occurred with a frequency of more than 20% in viral hemagglutinin (HA). Three of these mutations led to an amino acid change in viral proteins.. Two HA mutations, HA1 D130E and HA2 I91L, caused significant changes to the A549 receptor-binding affinity. The mutation HA1 D130E affects the HA globular head near the cell receptor ...
The selective binding between avian and human influenza A viral hemagglutinins (HA) subtype H3 and Neu5Acα2-3 and α2-6Gal (avian α2-3, human α2-6) is ...
Antigenic determinants of influenza virus haemagglutinin. VI. Antigenic characterization of the oligosaccharide sidechains from HA1of influenza virus haemagglutinins Journal Articles ...
Citation. Das SR, Hensley SE, Ince WL, Brooke CB, Subba A, Delboy MG, Russ G, Gibbs JS, Bennink JR, Yewdell JW. Defining Influenza A Virus Hemagglutinin Antigenic Drift by Sequential Monoclonal Antibody Selection.. Cell Host & Microbe. 2013 Mar 13; 13: 314-23.. External Citation. Abstract. Human influenza A virus (IAV) vaccination is limited by antigenic drift, rapid antibody-driven escape reflecting amino acid substitutions in the globular domain of hemagglutinin (HA), the viral attachment protein. To better understand drift, we used anti-hemagglutinin monoclonal Abs (mAbs) to sequentially select IAV escape mutants. Twelve selection steps, each resulting in a single amino acid substitution in the hemagglutinin globular domain, were required to eliminate antigenicity defined by monoclonal or polyclonal Abs. Sequential mutants grow robustly, showing the structural plasticity of HA, although several hemagglutinin substitutions required an epistatic substitution in the neuraminidase glycoprotein ...
In the polarized kidney cell line MDCK, the influenza virus hemagglutinin (HA) has been well characterized as a model for apically sorted membrane glycoproteins. Previous work from our laboratory has shown that a single amino acid change in the cytoplasmic sequence of HA converts it from a protein that is excluded from coated pits to one that is efficiently internalized. Using trypsin or antibodies to mark protein on the surface, we have shown in MDCK cells that HA containing this mutation is no longer transported to the apical surface but instead is delivered directly to the basolateral plasma membrane. We propose that a cytoplasmic feature similar to an endocytosis signal can cause exclusive basolateral delivery. ...
DNA vaccines expressing plasma membrane and secreted forms of the influenza and measles virus hemagglutinins (HAs) have been used to evaluate the effect of secretion on DNA-raised antibody responses. At low doses of DNA, the plasma membrane form of the influenza virus HA raised higher titers of antibody than the secreted form. The isotype of the DNA-raised antibodies depended on both the method of DNA delivery and the form of the expressed antigen. Following intramuscular injections, DNAs expressing membrane bound forms of the influenza and measles HAs raised predominantly IgG2a. By contrast, DNAs expressing the secreted from of the two HAs as well as another secreted protein, human growth hormone, raised predominantly IgG1. Gene gun delivery resulted in predominantly IgG1 antibody responses for both secreted and membrane bound forms of the hemagglutinins. The raising of predominantly IgG1 by i.m. delivery of the secreted form of the influenza hemagglutinin was IL-4 dependent suggesting that a T-helper
Author: Hütter, J. et al.; Genre: Journal Article; Published in Print: 2013; Title: Toward Animal Cell Culture-Based Influenza Vaccine Design: Viral Hemagglutinin N-Glycosylation Markedly Impacts Immunogenicity
Citation. Magadán JG, Khurana S, Das SR, Frank GM, Stevens J, Golding H, Bennink JR, Yewdell JW. Influenza a Virus Hemagglutinin Trimerization Completes Monomer Folding and Antigenicity.. Journal of Virology. 2013 Sep 01; 87: 9742-53.. External Citation. Abstract. Influenza A virus (IAV) remains an important human pathogen largely because of antigenic drift, the rapid emergence of antibody escape mutants that precludes durable vaccination. The most potent neutralizing antibodies interact with cognate epitopes in the globular head domain of hemagglutinin (HA), a homotrimeric glycoprotein. The H1 HA possesses five distinct regions defined by a large number of mouse monoclonal antibodies (MAbs), i.e., Ca1, Ca2, Cb, Sa, and Sb. Ca1-Ca2 sites require HA trimerization to attain full antigenicity, consistent with their locations on opposite sides of the trimer interface. Here, we show that full antigenicity of Cb and Sa sites also requires HA trimerization, as revealed by immunofluorescence ...
Influenza A virus H1 HA (Hemagglutinin) antibody [B219M] for ELISA, WB. Anti-Influenza A virus H1 HA (Hemagglutinin) mAb (GTX41203) is tested in Influenza A virus samples. 100% Ab-Assurance.
Influenza A virus H1N1 HA (Hemagglutinin) antibody [GT521] for WB. Anti-Influenza A virus H1N1 HA (Hemagglutinin) mAb (GTX629746) is tested in Influenza A virus samples. 100% Ab-Assurance.
Das Hüllglykoprotein Hämagglutinin (HA) von Influenzavirus ist verantwortlich sowohl für die Bindung als auch für die nachfolgende Fusion der viralen Hülle mit der endosomalen Membran. Eine Analyse der 3D Struktur der HA-Ektodomaine zeigt, dass die Stabilität des Proteins sowohl durch kovalente als auch durch nicht-kovalente Wechselwirkungen bedingt ist. Die Konformationsänderung von HA bei saurem pH-Wert weißt auf eine mögliche Rolle von Protonierungseffekten auf ionisierbare Aminosäuren hin. Untersuchungen zur Bedeutung geladener Aminosäuren und Salzbrücken für die Struktur des HA wurden auf der Grundlage von ‚site directed mutagenesis durchgeführt. Der Einfluss der Mutationen auf die Konformationsänderung und die Fusionsaktivität von HA wurden durch einen Proteinase K-Assay bzw. Fluoreszenzmikroskopie erfasst. Die Ergebnisse beider Methoden wurden miteinander korreliert. Abgesehen von der Mutante R109E zeigten Wildtyp-HA und alle anderen Mutanten eine vergleichbare ...
Das Hüllglykoprotein Hämagglutinin (HA) von Influenzavirus ist verantwortlich sowohl für die Bindung als auch für die nachfolgende Fusion der viralen Hülle mit der endosomalen Membran. Eine Analyse der 3D Struktur der HA-Ektodomaine zeigt, dass die Stabilität des Proteins sowohl durch kovalente als auch durch nicht-kovalente Wechselwirkungen bedingt ist. Die Konformationsänderung von HA bei saurem pH-Wert weißt auf eine mögliche Rolle von Protonierungseffekten auf ionisierbare Aminosäuren hin. Untersuchungen zur Bedeutung geladener Aminosäuren und Salzbrücken für die Struktur des HA wurden auf der Grundlage von ‚site directed mutagenesis durchgeführt. Der Einfluss der Mutationen auf die Konformationsänderung und die Fusionsaktivität von HA wurden durch einen Proteinase K-Assay bzw. Fluoreszenzmikroskopie erfasst. Die Ergebnisse beider Methoden wurden miteinander korreliert. Abgesehen von der Mutante R109E zeigten Wildtyp-HA und alle anderen Mutanten eine vergleichbare ...
Rabbit polyclonal antibody raised against synthetic peptide of Seasonal H1N1 Hemagglutinin. A synthetic peptide corresponding to Seasonal H1N1 Hemagglutinin. (PAB16771) - Products - Abnova
Shop Hemagglutinin/proteinase ELISA Kit, Recombinant Protein and Hemagglutinin/proteinase Antibody at MyBioSource. Custom ELISA Kit, Recombinant Protein and Antibody are available.
Hemagglutinin consists of a globular head and a stem. The globular head consists of three chains, Chains A, C, and E. The stem of the protein consists of three chains as well, Chains B, D, and F.
Biochemical tests used in the identification of infectious agents include the detection of metabolic or enzymatic products characteristic of a particular infectious agent. Since bacteria ferment carbohydrates in patterns characteristic of their genus and species, the detection of fermentation products is commonly used in bacterial identification. Acids, alcohols and gases are usually detected in these tests when bacteria are grown in selective liquid or solid media. The isolation of enzymes from infected tissue can also provide the basis of a biochemical diagnosis of an infectious disease. For example, humans can make neither RNA replicases nor reverse transcriptase, and the presence of these enzymes are characteristic of specific types of viral infections. The ability of the viral protein hemagglutinin to bind red blood cells together into a detectable matrix may also be characterized as a biochemical test for viral infection, although strictly speaking hemagglutinin is not an enzyme and has no ...
Biochemical tests used in the identification of infectious agents include the detection of metabolic or enzymatic products characteristic of a particular infectious agent. Since bacteria ferment carbohydrates in patterns characteristic of their genus and species, the detection of fermentation products is commonly used in bacterial identification. Acids, alcohols and gases are usually detected in these tests when bacteria are grown in selective liquid or solid media. The isolation of enzymes from infected tissue can also provide the basis of a biochemical diagnosis of an infectious disease. For example, humans can make neither RNA replicases nor reverse transcriptase, and the presence of these enzymes are characteristic., of specific types of viral infections. The ability of the viral protein hemagglutinin to bind red blood cells together into a detectable matrix may also be characterized as a biochemical test for viral infection, although strictly speaking hemagglutinin is not an enzyme and has ...
Biochemical tests used in the identification of infectious agents include the detection of metabolic or enzymatic products characteristic of a particular infectious agent. Since bacteria ferment carbohydrates in patterns characteristic of their genus and species, the detection of fermentation products is commonly used in bacterial identification. Acids, alcohols and gases are usually detected in these tests when bacteria are grown in selective liquid or solid media. The isolation of enzymes from infected tissue can also provide the basis of a biochemical diagnosis of an infectious disease. For example, humans can make neither RNA replicases nor reverse transcriptase, and the presence of these enzymes are characteristic., of specific types of viral infections. The ability of the viral protein hemagglutinin to bind red blood cells together into a detectable matrix may also be characterized as a biochemical test for viral infection, although strictly speaking hemagglutinin is not an enzyme and has ...
Ready-to-use high expression level Hemagglutinin/HA cDNA clones are full sequence confirmed with various fusion tags, 27 in lentiviral vector, 171 in expression vector, 2 in cloning vector also available.
The 12CA5 monoclonal antibody recognizes the 9-amino acid sequence YPYDVPDYA, derived from influenza virus hemagglutinin (HA). HA is commonly added to proteins of interest using recombinant DNA technology. The HA tag can then be used for detection or purification of the tagged protein.
p>An evidence describes the source of an annotation, e.g. an experiment that has been published in the scientific literature, an orthologous protein, a record from another database, etc.,/p> ,p>,a href="/manual/evidences">More…,/a>,/p> ...
除此之外3初步的分析還指出3這種病毒不會在鳥類身上引起嚴重的症狀3這根自2002起蹂躪亞洲的H5N1病毒株不同》雖然不會在鳥類身上引起嚴重的症狀3但是在人類身上就會3因為我們對這種新病毒毫無免疫力4此外3初步研究指出3這種新型病毒會感染下呼吸道的深層3而這種感染3就像引起SARS的新型冠狀病毒一樣3會引起嚴重的疾病》另外3這種病毒如何跟人們呼吸道上的蛋白質接受器結合也跟它引起疾病的嚴重程度有關3而科學家們還需要進一步研究4雖然都是非常初步的3但分析已經指出3該病毒的H蛋白(haemagglutinin(已經產生了突變使得這個蛋白質可以跟哺乳動物呼吸道上的接受器結合3而且3該病毒還具有數個突變跟哺乳動物的嚴重疾病有關》 ...
Sasisekharan noted that recent studies have shown that the hemagglutinin protein from the avian flu virus has on occasion to glycans of the upper airway. The
Zhang X., Lu G., Qi J., Li Y., He Y., Xu X., Shi J., Zhang C.W., Yan J., Gao G.F.. Measles virus is a major public health concern worldwide. Three measles virus cell receptors have been identified so far, and the structures of the first two in complex with measles virus hemagglutinin (MV-H) have been reported. Nectin-4 is the most recently identified receptor in epithelial cells, and its binding mode to MV-H remains elusive. In this study, we solved the structure of the membrane-distal domain of human nectin-4 in complex with MV-H. The structure shows that nectin-4 binds the MV-H β4-β5 groove exclusively via its N-terminal IgV domain; the contact interface is dominated by hydrophobic interactions. The binding site in MV-H for nectin-4 also overlaps extensively with those of the other two receptors. Finally, a hydrophobic pocket centered in the β4-β5 groove is involved in binding to all three identified measles virus receptors, representing a potential target for antiviral drugs.. Nat. ...
cansSAR 3D Structure of 5VTQ_B | CRYSTAL STRUCTURE OF THE A/HONG KONG/1/1968 (H3N2) INFLUENZA VIRUS HEMAGGLUTININ G225L/L226S MUTANT IN COMPLEX WITH 3-SLN | 5VTQ
Define haemagglutinin. haemagglutinin synonyms, haemagglutinin pronunciation, haemagglutinin translation, English dictionary definition of haemagglutinin. or n an antibody that causes the clumping of red blood cells
1HGD: BINDING OF INFLUENZA VIRUS HEMAGGLUTININ TO ANALOGS OF ITS CELL-SURFACE RECEPTOR, SIALIC ACID: ANALYSIS BY PROTON NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY AND X-RAY CRYSTALLOGRAPHY
Mouse monoclonal antibody raised against Influenza B virus hemagglutinin. B/Lee/40 and B/Singapore/-222/79 virus. (MAB14108) - Products - Abnova
There are no specific protocols for Recombinant Influenza A Virus Hemagglutinin H5 protein (ab69748). Please download our general protocols booklet
Using the Octet RED system, Kd values for S139/1 Fab and IgG binding to various influenza HA strains were determined to assess enhanced virus neutralization effects due to avidity of full IgG vs. Fab. Biotinylated HA and Streptavidin biosensors were used for these measurements. The increased avidity of IgG binding was shown to reduce Kd values and improve ability of S139/1 to recognize different heterosubtypes of the virus. The Kd data correlated with neutralization data.
1HGD: Binding of influenza virus hemagglutinin to analogs of its cell-surface receptor, sialic acid: analysis by proton nuclear magnetic resonance spectroscopy and X-ray crystallography.
GPM.1.8.2.0005.15 Determination of anti-A and anti-B haemagglutinins in medicinal products containing human immunoglobulins Ministry of Health of the Russian
Over 22 years ago, the August 1995 issue of the Journal of Virology published a study very similar to the one being discussed today called Selection of a Single Amino Acid Substitution in the Hemagglutinin Molecule by Chicken Eggs Can Render Influenza A Virus (H3) Candidate Vaccine Ineffective. The study essentially concluded that thanks to mutations, (which are extremely common), growing these viruses in mutated eggs should be ended. "Thus, it is recommended that in the selection of vaccine candidates, virus populations with the egg-adapted HA Lys-156 substitution be eliminated." Yes, I realize that this is a bit different mutation than the one we were discussing earlier, but the principles are the same. Said mutations caused a vaccine that was described with words such as "nonprotective," "poorly recognized," and that, "egg-grown HA Lys-156 variant induced an AFC profile vastly different from that elicited by the other two reassortant vaccines." With this being the case, why should be be ...
mouse Anti-HA, conjugated to DyLight 550 antibodies, anti-HA conjugated to DyLight 550, directly conjugated anti-HA antibody, AS15 2920, Human influenza hemagglutinin (HA) tagged proteins
INFLUENZA HEMAGGLUTININ PROTEINS AND METHODS OF USE THEREOF | TOLL-LIKE RECEPTOR 2 AGONISTS AND VACCINES AND USES THEREOF | Multivalent VLP Conjugates | SALMONELLA CHOLERAESUIS-SALMONELLA TYPHIMURIUM VACCINES | EHRLICHIAL INVASIN FOR IMMUNIZTION, DIAGNOSIS, AND CELL DELIVERY |
The monoclonal anti‐HA Tag antibody, clone HA‐1A1, recognises HA, and HA tagged proteins. Human influenza hemagglutinin (HA) is a
HA Epitope Tag (YPYDVPDYA), 0.1 mg. Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus.
HA Epitope Tag (YPYDVPDYA), 0.1 mg. Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus.
SWISS-MODEL Template Library (SMTL) entry for 6q18.1. Human antibody H1244 in complex with the influenza hemagglutinin head domain of A/Beijing/262/95(H1N1)
Scientists have developed a universal cure for the flu 2016. The new drug is effective since it destroys the virus proteins, ie hemagglutinin and neuraminidase. American ...
Nishizuka, Y; Tanaka, Y; Sakakura, T; and Kojima, A, "Frequent development of ovarian tumors from dysgenetic ovaries of neo- natally thymectomized mice." (1972). Subject Strain Bibliography 1972. 1525 ...