Hypoxia plays a key role in tumour cell survival, invasion, and metastasis. An increasing number of studies have attempted to characterize the tumour response to hypoxia and to identify predictive markers of disease. Here we show that hypoxia increases tumour cell invasion and migration by the modulation of Rab11, an important molecule for vesicular trafficking. In our study, we found that Rab11, together with the activation of Rac1, could stimulate invasion and migration of cervical cancer cell lines HeLa/SiHa in hypoxia. Activation of Rac1 activity by hypoxia seems to be central to carcinoma invasion. We also found that these effects could be related to the integrin αvβ3. In addition, we studied the molecular pathway for this process. Our results showed that in cervical cancer cell... lines HeLa/SiHa, Rac1 activation in hypoxia could stimulate invasion and migration, and this process was mediated by integrin αvβ3-mediated FAK and PI3K phosphorylation. Furthermore, hypoxia induced a ...
BioAssay record AID 87350 submitted by ChEMBL: Compound was tested for antiviral activity in HeLa Cell cultures against herpes simplex virus type 1 (HSV-1).
HeLa cells in culture. Light microscopy footage of the movement, division and death of HeLa cervical cancer cells. HeLa cells are a continuously cultured cell line of human cancer cells. They are immortal, in that they can divide an unlimited number of times. However, they are also poorly differentiated and lack some of the characteristics of normal cells. HeLa cells are widely used in biological and medical research. They all originate from a sample taken in 1951 from cervical cancer patient Henrietta Lacks, after whom they are named. - Stock Video Clip K005/6160
HeLa cell. Coloured scanning electron micrograph (SEM) of a cultured Hela cell (centre) on MDCK kidney cells (background). HeLa cells are a continuously cultured cell line of human cancer cells, which are immortal and so thrive in the laboratory. Numerous microvilli (string-like) can be seen, which cancer cells use to enable them to move around and spread (metastasise) from one area to another. MDCK cells are a strain of cultured, normal kidney epithelial cells. - Stock Image C011/1346
BioAssay record AID 506767 submitted by ChEMBL: Binding affinity to SAP145 in SAP145-targeting siRNA-treated human HeLa cells at 1 uM by fluorescent microscopy.
Accumulating evidence supports the concept that cancer stem cells (CSCs) are responsible for tumor initiation and maintenance. They are also considered as an attractive target for advanced cancer therapy. Using a sphere culture method that favors the growth of self-renewal cells, we have isolated sphere-forming cells (SFCs) from cervical cancer cell lines HeLa and SiHa. HeLa-SFCs were resistant to multiple chemotherapeutic drugs and were more tumorigenic, as evidenced by the growth of tumors following injection of immunodeficient mice with 1 × 104 cells, compared with 1 × 106 parental HeLa cells required to grow tumors of similar size in the same time frame. These cells showed an expression pattern of CD44high/CD24low that resembles the CSC surface biomarker of breast cancer. We further demonstrated that HeLa-SFCs expressed a higher level (6.9-fold) of the human papillomavirus oncogene E6, compared with that of parental HeLa cells. Gene silencing of E6 with a lentiviral-short-hairpin RNA (shRNA)
Fig. 6. Mitotic events of cell division after disruption of mitochondria in the histone EGFP-H1 expressed cell. The disruption of a single mitochondrion by femtosecond laser irradiation had no influence on cell division or cell activity. The cell nuclei and mitotic chromosomes in HeLa cells were visualized using histone EGFP-H1. Mitochondria were stained with MitoTracker Red. Confocal fluorescence image and transmission image (a) before and (b) after femtosecond laser irradiation with 0.39 nJ/pulse (exposure time: 32 ms). The yellow arrow indicates the irradiation point. (c)-(f) Time-lapse confocal images and transmission images. The mitotic events of cell division in the irradiated cells proceeded normally. Scale bar: 20 μm ...
Im pretty sure the fact that HeLa cells are abnormal has been known more or less since they were first isolated. That said, how they are abnormal has not. And this is very relevant - if researchers are using HeLa cells to study something, and HeLa cells are abnormal along that axis, then their data are suspect. So knowing how they are normal, and abnormal, is important information. This is why the news that the transformation of healthy non-cancerous cervical cells into HeLa cells happened due to human papilloma virus infection - that is, Henrietta Lacks (the source of the cells) had HPV, which gave her the cancer that ultimately killed her. This is noteworthy because it establishes the mutation(s) and phenotype(s) that result when cervical cells are infected with HPV (with ramifications both for work with HeLa and other cervical cancer lines AND for care for patients).. Remember also that HeLa was the very first immortalized cell line. Studies on HeLa led to the immortalization of other cells, ...
Light micrograph of dividing HeLa cells. Chromosomes in the cell nucleus (purple), microtubules in the cell cytoskeleton (tubulin; green), and actin (red) are visible here. In the cell in the centre of the image, condensed chromosomes (purple) have aligned and attached to the spindle (green). During anaphase (one of the stages of nuclear division in mitosis), the spindle pulls the condensed chromosomes to opposite poles of the cell. The cell will finish dividing its nuclear material and then cytoplasm in order to physically separate into two daughter cells. HeLa cells are an immortal human epithelial cell line derived from a cancerous tumour of the cervix (adenocarcinoma). It was established in 1951 from a biopsy taken from Henrietta Lacks and was the first human cell line to survive and grow in the laboratory. Henriettas cells were originally used in this way without permission from her or her family which raises issues about ethics and privacy. HeLa cells have been used extensively around the world
Definition of HeLa cell in the Legal Dictionary - by Free online English dictionary and encyclopedia. What is HeLa cell? Meaning of HeLa cell as a legal term. What does HeLa cell mean in law?
Background Cervical cancer is one of the most common cancers in women worldwide. Malignant tumors develop resistance mechanisms and are less sensitive to or do not respond to irradiation. With the development of high-throughput sequencing technologies, circular RNA (circRNA) has been identified in an increasing number of diseases, especially cancers. It has been reported that circRNA can compete with microRNAs (miRNAs) to change the stability or translation of target RNAs, thus regulating gene expression at the transcriptional level. However, the role of circRNAs in cervical cancer and the radioresistance mechanisms of HeLa cells are unknown. The objective of this study is to investigate the role of circRNAs in radioresistance in HeLa cells. Methods High-throughput sequencing and bioinformatics analysis of irradiated and sham-irradiated HeLa cells. The reliability of high-throughput RNA sequencing was validated using quantitative real-time polymerase chain reaction. The most significant circRNA
This study demonstrated the cytotoxic and cell growth inhibitory effect of a bioactive extract of |i|Funalia trogii|/i| (Berk.) Bond. et Singer, which was grown...
Relying on virtual screening and the 3-dimensional structure of the complex of PAI-1 with its inhibitory peptide, we have identified 2 novel, orally bioavailable, small molecule PAI-1 inhibitors, TM5001 and TM5007. Both are stable, nontoxic, and devoid of cellular toxicity as demonstrated in vitro by their inability to raise LDH levels in the medium of cultured HeLa cells. The absence of acute and subacute toxicity is confirmed in vivo in mice given a single dose of up to 2000 mg/kg, or in rats fed 300 mg/kg for 1 week or 2000 mg/kg for 2 weeks. The in vivo effectiveness of TM5007 is demonstrated in animal models of either acute vascular thrombosis or of chronic lung fibrosis, without deleterious effects on blood pressure or bleeding, in good agreement with previous results in PAI-1 deficient mice and humans.8,14. The specificity of the effect of TM5007 on PAI-I was further documented in other serpin/serine protease systems (ie, α1-antitrypsin/trypsin and α2-antiplasmin/plasmin) by a ...
Relying on virtual screening and the 3-dimensional structure of the complex of PAI-1 with its inhibitory peptide, we have identified 2 novel, orally bioavailable, small molecule PAI-1 inhibitors, TM5001 and TM5007. Both are stable, nontoxic, and devoid of cellular toxicity as demonstrated in vitro by their inability to raise LDH levels in the medium of cultured HeLa cells. The absence of acute and subacute toxicity is confirmed in vivo in mice given a single dose of up to 2000 mg/kg, or in rats fed 300 mg/kg for 1 week or 2000 mg/kg for 2 weeks. The in vivo effectiveness of TM5007 is demonstrated in animal models of either acute vascular thrombosis or of chronic lung fibrosis, without deleterious effects on blood pressure or bleeding, in good agreement with previous results in PAI-1 deficient mice and humans.8,14. The specificity of the effect of TM5007 on PAI-I was further documented in other serpin/serine protease systems (ie, α1-antitrypsin/trypsin and α2-antiplasmin/plasmin) by a ...
Cell and Culture Conditions. The PKR wild-type MEFs [MEF/PKR(+/+)] and PKR-knockout MEFs [MEF/PKR(-/-)], provided by Dr. Glen Barber (University of Miami, Miami, FL), have been described previously (Yang et al., 1995). The human cervical cancer cell line HeLa and the human lung cancer cell lines H1299, H460, H226B, and A549 were maintained in our laboratory. The human bronchial epithelial (HBE) cell line was purchased from Clonetics (Walkersville, MD). All cells except for HBE cells were routinely cultured in Dulbeccos modified Eagles medium supplemented with 10% heat-inactivated fetal calf serum, 100 units/ml penicillin, and 100 mg/ml streptomycin and maintained in the presence of 5% CO2 at 37°C. HBE cells were cultured in serum-free keratinocyte medium (Invitrogen, Carlsbad, CA).. Chemicals and Antibodies. A chemical library with 10,000 compounds, including BEPP and its analog BECC, was obtained from ChemBridge Research Laboratories, Inc.. The chemicals in the library were provided at a ...
One cryopreserved vial of HeLa NucLight Red (nuclear-labelled RFP) expressing cells at a density of 1x106 cells/vial. Each vial contains a stable population of 1 million HeLa cells expressing the NucLight Red fluorescent protein restricted to the nucleus. Parental HeLa cells were purchased from ATCC (Cat# CCL-2). HeLa
Although it is widely believed that eukaryotic DNA is looped by attachment to a nucleoskeleton, there is controversy about its composition and which sequences are attached to it. As most nuclear derivatives are isolated using unphysiological conditions, the criticism that attachments seen in vitro are generated artifactually has been difficult to rebut. Therefore we have re-investigated attachments of chromatin to the skeleton using physiological conditions. HeLa cells are encapsulated in agarose microbeads and lysed using Triton in a physiological buffer. Then, most chromatin can be electroeluted after treatment with a restriction enzyme to leave some at the base of the loops still attached. Analysis of the size and amounts of these residual fragments indicates that the loops are 80-90kbp long. The residual fragments are stably attached, with about 1kbp of each fragment protected from nuclease attack. This is very much longer than a typical protein-binding site of 10-20bp.
While both the Rabenosyn-5 and Vps45 orthologs appear to coordinate endocytic regulatory functions in yeast and invertebrates, their relationship in mammalian cells is not well understood. Leaving out -L would be equivalent to L0, or specifying that the pool level was backup01. To assess the impact of TBC1d5 loss on VPS35 recruitment to endosomes, TBC1d5 KO cells were mixed with control HeLa cells at a ratio of 1:1 and the cells were simultaneously stained with TBC1d5, VPS35 and EEA1. 1 ). This approach, used by most system emulators, allows the emulator to run an arbitrary guest operating system without modifications because guest OS is not aware that it is not running on real hardware. This is consistent with a proposed role for the vps pathway in VP40 vesicular release. Untuk konsultasi konfigurasi server custom isp for a dns server mendapatkan diskon terbaik (untuk pembelian cloud resources dalam jumlah banyak), silahkan hubungi free terraria server download. BlueHost grew enormously in the ...
INA-UV induced apoptosis in Hela cells is also caspase dependent. A. Hela cells, pre-incubated or not with 40 μM ZVADfmk were treated with indicated amounts of
HeLa cells were washed with cold PBS and lysed in cell lysis buffer (20-mM Tris-HCl, pH 8.0, 150-mM NaCl, 2-mM EGTA, 0.5-mM EDTA, 0.5% NP-40, 5-mM NaF, 1-mM Na3VO4, 1-mM PMSF, and 500× protease inhibitor cocktail; Calbiochem) for 20 min on ice. For Usp16/Plk1 coIP assays, cell lysates were mixed with anti-Usp16 polyclonal antibodies (raised in the laboratory in rabbit using His-tagged Usp16 1-257 aa) or mouse anti-Plk1 antibodies (06-813; EMD Millipore) and 15 µl protein A-Sepharose beads (75% slurry) and incubated at 4°C for 1 h. After washing with lysis buffer, the beads were harvested and suspended in Laemmli sample buffer. For Plk1/BubR1 coIP assays, cell lysates were mixed with anti-Plk1 polyclonal antibodies (raised in the laboratory in rabbit using His-tagged Plk1 326-end). For GST pull-down assays, HeLa cell lysates were incubated with 5 µg of soluble GST or GST-fused proteins bound to 15 µl glutathione-Sepharose beads (75% slurry) at 4°C for 1 h. After washing with lysis buffer, ...
Cell lines are an invaluable scientific tool. They allow us to dissect the internal workings of tissues in a controlled environment without the ethical implications of working with whole organisms. Starting with the first successful immortal cell line HeLa, the number of available cell lines has since diversified into a plethora of options. Just like model […]. The post Top 5 of the most commonly used cell lines! appeared first on Bitesize Bio.. ...
AC0206: Western Blot (1:1000) of over-expressed TMX with positive and negative controls on HeLa Cells. TMX is the 34kDa band. The upper band (~38 kDa) is myc-tagged TMX. The lower band at~28 kDa (*) is a non-specific band.. ...
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Kinetics of B-fragment transport from EE/RE to the Golgi apparatus. (A) Confocal microscopy on living HeLa cells. Fluorophore-labeled B-fragment was internalize
Here we present a new approach to aqueous hydrazoic acid detection through the synthesis and evaluation of an alkyne-based fluorescent probe, which could be applied in the monitoring of hydrazoic acid in both living Hela cells and larval zebrafish. This probe can also serve as an early warning automaton whic
目的:探讨反义脱氧寡核苷酸封闭HSP70基因对体外宫颈癌 HeLa 细胞增殖、凋亡及化疗敏感性的影响。方法:1)将体外培养的宫颈癌 HeLa 细胞分为正常对照组(Ctrl组)、反义寡核苷酸处理组(AS组)、正义寡核苷酸处理组(S组)、随机寡核苷酸处理组(R组),每组各5例,分别转染体外培养的宫颈癌 HeLa 细胞,采用Western免疫印迹检测各组细胞HSP70蛋白表达。2)将顺铂处理体外培养的宫颈癌 HeLa 细胞分为正常对照组(Ctrl组)、单纯顺铂处理组(Cis组)、反义寡核苷酸+顺铂处理组(AS +Cis组)、正义寡核苷酸+顺铂处理组(S +Cis组)、随机寡核苷酸+顺铂处理组 (R +Cis组),每组各8例。采用四甲基偶氮唑蓝光吸收法(methyl thiazolyl tetrazolium,MTT) 法检测 HeLa 细胞的生长抑制率; 流式细胞术检测 HeLa 细胞的凋亡率。结果:1)Ctrl组、AS组、S组、R组HSP70灰度比值分别为1.365±0.187,0.379±0.134,1.403±0.163和1.410±0
When Professor Gertrude Buehring steps into her lab in Koshland Hall, she is never met by HeLa cells. In fact, she makes a point of it. "We never grow them," she says. "I wouldnt want to take that risk, actually.". Buehring, a professor of virology in the School of Public Health, has a reason to be wary of HeLa. Both her PhD and postdoc careers were spent working at UC Berkeleys Cell Culture Laboratory housed in the Naval Biosciences Laboratory in Oakland, a cell repository funded by the federal government that characterized and maintained cell lines for research scientists. She happened to be working there at a time when Dr. Walter Nelson-Rees, the co-director, was working hard to expose HeLas misdeeds. The vigorous cells crime? The contamination of other, less hardy cell lines.. Nelson-Rees was not the first to suspect contamination by HeLa cells. In the 1960s, Dr. Stanley Gartler, a research geneticist, released the initial "HeLa bomb". Gartler had discovered that the 18 different cell ...
This paper reviews several different cell types and cell preparation approaches using standard cells, such as, CHO cells, EL4 cells, SF9 cells, and HELA cell cultures.
Ran-Binding Protein M (RanBPM) has been previously shown to inhibit c-Raf expression, however how this was achieved remains unclear. c-Raf is the central component of the extracellular signal-regulated kinase (ERK) pathway which has been linked to many cancer types. Furthermore, RanBPM was recently identified as part of the E3 ubiquitin ligase complex and the CTLH (C-terminal to LisH) complex (McTavish et al., 2019). Lastly, RanBPM has been linked to various signaling pathways related to numerous cellular processes which include - apoptosis, cell adhesion, migration, transcription, nuclear-cytoplasmic transport and also plays a significant role during development (Salemi et al., 2017). ...
Human IFITM1 Knockout HeLa Cell Lysate parental control lysate. CRISPR/Cas9 generated negative control for antibody validation in WB.
The assembly of a rotating HeLa cell/gold rod aggregate at an acoustic nodal line in the xy plane. The video was taken under 500X overall magnification except for 00:23 - 00:32 and 01:16 - 01:42, where a 200X overall magnification was used.
TY - JOUR. T1 - Anomalous structures in the cytoplasm of HeLa cells cultured in the presence of 5-bromodeoxvuridine.. AU - HENDEE, W. R.. AU - ZEBRUN, W.. AU - BONTE, F. J.. PY - 1963/6. Y1 - 1963/6. UR - http://www.scopus.com/inward/record.url?scp=84883840494&partnerID=8YFLogxK. UR - http://www.scopus.com/inward/citedby.url?scp=84883840494&partnerID=8YFLogxK. M3 - Article. C2 - 13953692. AN - SCOPUS:84883840494. VL - 17. SP - 675. EP - 680. JO - Journal of Cell Biology. JF - Journal of Cell Biology. SN - 0021-9525. ER - ...
Could anyone tell me the approximate percetage or amount of RNA in HeLa cell? I need around 50ugm for microarray experiment.Thanks a lot!. ...
Page contains details about gold nanoparticle-loaded HeLa cells . It has composition images, properties, Characterization methods, synthesis, applications and reference articles : nano.nature.com
LIF KO Cell Line. Mutation description: Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon2 and 32 bp deletion in exon2. KO validated by Sanger sequencing and western blot.
PSCA KO Cell Line. Mutation description: Knockout achieved by using CRISPR/Cas9, Homozygous: 2 bp deletion in exon 2. KO validated by Sanger sequencing and western blot.
Hela cell is an immortal cell line used in medical research. The cell line was derived...39;s diagnosis of cancer, but a debate still continues on the classification of the cells. HeLa are termed immortal in that they can divide an unlimited number of times as long... ...
We utilized the BAG-one promoter region of an 890-bp DNA as a probe to monitor the HeLa cDNA library, lTripIEx, by Southwestern blot investigation. Following
A good explanation is summarized in Cell and Tissue Culture: Lab ProceduresEds A. Doyle, JB Grifiths and DG Newell 27D:2.4. It is advisable to perform a death curve on the cells to be transfected, for 2 major reasons (even if results for your cell line have been published):1- The active component of geneticin (powdered form of G418) constitutes about 50% of the weight with variation between batches 2- Some groups quote active concentration while others quote net weight. To determine a suitable concentration for selection:Plate non-transfected control cells at a low density (1E4/ml for example)The next day, add G418 at 0, 100, 200, 400, 600, 800, 1000, 1200 ug/mlIncubate the cells for 6 days (may need to refeed)Check cells under microscope. The concentration of G418 which just kills all the cells is suitable for use in subsequent selection procedures.. ...
Henriettas cells were the first immortal human cells ever grown in culture. They were essential to developing the polio vaccine. Many scientific landmarks since then have used her cells, including cloning, gene mapping and in vitro fertilization. Continue reading →. ...
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The biological application of nanoparticles (NPs) is a rapidly developing area of nanotechnology that raises new possibilities in the treatment of human cancers. The cytotoxicity was evaluated by MTT and LDH assays. The apoptotic effect of free ICD-85 and ICD-85 NPs on HeLa cells was assessed using caspase-8 colorimetric assay. The MTT assay showed that ICD-85 NPs could enhance the in-vitro cytotoxicity against HeLa cellscompared to the free ICD-85. The IC50 value at 72 h was reduced from 25 ± 2.9 μg/mL for free ICD-85 to 15.5 ± 2.4 μg/mL for ICD-85 NPs. However, LDH assay demonstrated that ICD-85 has dose-dependent cytotoxicity on HeLa cells while ICD-85 NPs exhibited weaker cytotoxicity on same cells. The results also indicate that ICD-85-induced apoptosis on HeLa cells is associated with the activation of caspase-8. Moreover, caspase-8 assay analysis demonstrated that the ICD-85 NPs induced a higher apoptotic rate in HeLa cells compared to free ICD-85. Our results demonstrated that the
Background and AimCervical and breast cancer are considered to be the most common cause of death among Iranian women. Various studies have shown that probiotics are effective fighting cancer. The aim of the present study was to investigate the effect of Lactobacillus brevis on apoptosis and casp (casp3, casp8) gene expression in HeLa cancer cells. Materials and MethodsIn this study, L. brevis bacteria were collected from the vaginal fluid of females referred to Alzahra Hospital and isolated and cultured in MRS agar medium. Inhibition of HeLa cancer cell proliferation by bacteria was evaluated by MTT assay. Apoptosis of cancer cells was measured by fluorescent microscopy using DAPI method. Finally, expression of Caspase and akt genes was measured by Real time PCR.ResultsThe results indicated that L. brevis had the same effect on HeLa cancer cells (P=0.42, P=0.26). On the other hand, there was no significant increase in akt gene expression (p> 0.05).ConclusionLactobacillus brevis bacteria can be
NDRG2, a member of N-Myc downstream regulated gene family, plays some roles in cellular stress, cell differentiation and tumor suppression. We have found that NDRG2 expression in cervical cancer Hela cells increases significantly upon stimulation with cisplatin, the most popular chemotherapeutic agent currently used for the treatment of advanced cervical cancer. This interesting phenomenon drove us to evaluate the role of NDRG2 in chemosensitivity of Hela cells. In the present study, RNA interference was employed to down-regulate NDRG2 expression in Hela cells. RT-PCR and Western blot were used to detect expression of NDRG2, Bcl-2 and Bax in cancer cells. Real-time PCR was applied to detect miR-15b and miR-16 expression levels. Drug sensitivity was determined with MTT assay. Cell cloning efficiency was evaluated by Colony-forming assay. Apoptotic cells were detected with annexin V staining and flow cytometry. In vitro drug sensitivity assay revealed that suppression of NDRG2 could sensitize Hela cells
Epithelial-to-mesenchymal transition (EMT) renders cervical cancer cells more susceptible to metastasis, and reduced expression levels of E-cadherin have been shown in this malignancy (9). E-cadherin is a pivotal molecule involved in epithelial cell adhesion, aberrant expression of which has been demonstrated in various human malignancies as a result of irreversible (genetic) and reversible (epigenetic) modifications of the E-cadherin gene (CDH1), such that the epigenetic ones have been more implicated in E-cadherin downregulation (10).. In this study, we evaluated the impact of oxamflatin on HeLa cell line by determining the expression level of E-cadherin. Our results demonstrated that oxamflatin treatment leads to a time- and concentration-dependent increase in E-cadherin expression in cervical cancer cell lines.. There is ample evidence reporting that HDACIs could be applied to re-express silenced genes (9, 11). For example, valproic acid (VPA), as a HDAC inhibitor, led to the re-expression ...
TY - JOUR. T1 - Fabrication of thermo-responsive PNIPAAm-g-ETFE for cell culture dishes by pre-irradiation grafting. AU - Yamahara, Yumi. AU - Nagasawa, Naotsugu. AU - Taguchi, Mitsumasa. AU - Oshima, Akihiro. AU - Washio, Masakazu. PY - 2018/1. Y1 - 2018/1. N2 - Thermo-responsive templates for the cell cultivation based on Poly(tetrafluoroethylene-co-ethylene) (ETFE) were fabricated by pre-irradiation grafting of N-isoproplyacrylamide (NIPAAm) monomer by electron beam (EB) irradiation under nitrogen gas atmosphere at room temperature, and their characteristic properties were studied. The detachment of cultured HeLa cells from fabricated thermo-responsive templates were attempted. Furthermore, the reaction mechanism is proposed using ESR spectroscopy and FT-IR spectroscopy. It is confirmed that the cultured HeLa cells were detached from fabricated thermo-responsive templates at 20 °C. Water contact angle analysis indicated that obtained templates had thermo-response around 30 °C. It is ...
Carcinoma of the cervix is the second most common cancer among women worldwide especially in the developing world. Due to severe reported cytotoxic tendenciesby anticancer drugs, newer therapies from natural products are desirable. This work therefore aims to partially purify pro-apoptotic agents from indigenous natural products and to investigate the mechanism of action of the selected plant fractions.Sixteen indigenous medicinal plants were collected from South West Nigeria. Plant extracts were fractionated into hexane (HF), chloroform (CF), ethylacetate (EAF), methanol (MF) and water (WF) fractions using solvent-solvent partitioning and polyamide adsorption chromatography. The resultant ninety-six test fractions were screened for lethality potential using brine shrimp lethality (BSL) assay. Fractions with LC50 less than 10 ug/ml were subjected to Water Soluble Tetrazolium (WST-1) cytotoxicity assay and apoptosis evaluations using: morphological assessment, flow cytometric analysis of ...
The tumor promoter 12-O-tetradecanoylphorbol-13-acetate (TPA) caused a marked stimulation of inorganic [32P]orthophosphate incorporation into HeLa-cell phosphatidylcholine (PC), phosphatidylethanolamine (PE), and lysophosphatidylethanolamine. The increased incorporation of inorganic [32P]orthophosphate into PE and lysophosphatidylethanolamine in the presence of TPA was not associated with an increase in PE synthesis as detected by the incorporation of [3H]serine or [3H]ethanolamine. The PC-specific exchange protein from beef liver was used to insert PC labeled with [3H]choline, inorganic [32P]orthophosphate, or [14C]arachidonic acid plus [3H]palmitic acid into the outer monolayer of intact HeLa cell membranes. Radioactivity from the latter two compounds was rapidly incorporated into PE and lysophosphatidylethanolamine; the incorporation was stimulated by TPA. It was concluded that TPA stimulated the formation of PE by base exchange between ethanolamine and PC.. ...
This is too long for a comment, so I have to write it in here:. Mostly this is because other cell lines are more practical in the lab.. Stem cells are much more tricky to maintain - especially if you want to keep their stem cell properties. They only grow very slow, tend to differentiate when they get too much stress, cellular signals and so on and need special media (these need to be defined, so that they are not containing factors which promote differentiation for example). For everyday experiments (like expression profiles, experiments on the effects of certain substances on the growth, gene expression or whatever of cells) "normal" cell lines are much more suited as these are much easier to keep in the lab using standard media. Additionally,experiments can be more easily repeated and reproduced, since these cells should behave always the same.. ...