Snippe, H; Houte, A V.; and Merchant, B, "Immunogenic synergy of haptenated liposomes and haptenated hemocyanin in b-cell defective cba/n mice. Abstr." (1982). Subject Strain Bibliography 1982. 2073 ...
Exposure of normal mouse spleen cells in vitro to highly 125I-labeled dinitrophenyl (DNP)-protein carrier conjugates specifically inactivated cells able to mount an immune response to that hapten after in vivo challenge. The deletion was hapten specific and independent of the radioactive carrier to which the hapten was bound. DNP-binding cells were inactivated by radioactivity that was not part of the hapten, but was solely confined to the carrier moiety. The deletion of the anti-DNP response lasted 2-3 wk and could be specifically inhibited.. ...
Anti-ϑ serum treatment of cells from immunized mice before their transfer to irradiated non-immune recipients decreased the ability of such cells to be stimulated by hapten on both homologous and heterologous carriers over a wide range of antigen doses. The anti-ϑ treatment was shown to affect only thymus-dependent (T) cells, since the number of bone marrow-derived (B) cell precursors was unaltered. Thus, specific T cells contribute significantly, not only to stimulation by the hapten on the homologous carrier, but also when hapten is presented on heterologous and even poorly immunogenic carriers. These results are interpreted as indicating a role for collaborative interactions of hapten-specific T cells with B cells specific for the same determinant. Such interactions were found at low antigen concentration. Results are discussed with relation to the mechanism of B cell stimulation.. ...
Rockey, J H.; Montogmery, P C.; and Dorrington, K J., "Induced optical activity (circular dichroism) of antibody-hapten complexes. Abstr." (1971). Subject Strain Bibliography 1971. 2145 ...
Competitive immunoassay-validated anti-Hapten antibody against hapten [Salbutamol(SAL)] and hapten-carrier conjugates [BSA-Salbutamol(SAL)] competitive antigens(Carrier-coupled antigen, immunogen, hapten-carrier conjugates, BSA-conjugated, OVA-conjugated). The anti-Hapten antibody against hapten [Salbutamol(SAL)] had been validated with our hapten-carrier conjugates [BSA-Salbutamol(SAL)]via competitive ELISA test. GeneMedi offers paired anti-small molecules (Chemicals, Antibiotics, Mycotoxins, Hormones, Drugs of Abuse , etc.) antibodies (monoclonal antibody, mab)and small molecules competitive antigens (Carrier-coupled antigen, immunogen, hapten-carrier conjugates, BSA-conjugated , OVA-conjugated) for the rapid test and diagnostics kit of Growth Promoters, Toxic Heavy Metal, Myotoxins, Nutritions, Food Safety, Pesticides, Drugs of Abuse, Allergen, Antibiotics, Agricultural, Water Contamination and so on. All our anti-chemicals antibodies and small molecules hapten-carrier conjugates antigens (BSA
Looking for hapten? Find out information about hapten. An incomplete antigen that cannot induce antibody formation by itself but can do so by coupling with a larger carrier molecule Explanation of hapten
The present studies were designed to probe the role(s) of T cells in preventing or altering tolerance induction in hapten-specific B cells. This was accomplished by using hapten conjugates of normally immunogenic heterologous carriers to selectively inhibit 2,4-dinitrophenyl (DNP)-primed B cells in adoptive transfer experiments in vivo. The data provide strong indications that one critical role of T-cell participation in humoral responses to antigens is to circumvent the development of a tolerogenic signal that, in the absence of such T-cell function, might otherwise ensue after binding of the antigenic determinants by specific precursor B lymphocytes.. ...
Mesenteric lymph node cells were obtained from rabbits that were primed with dinitrophenylated ragweed antigen (DNP-Rag) and supplemented with free Ascaris antigen (Asc); in vitro anti-hapten antibody response of the primed cells was studied. It was found that stimulation of Rag-sensitized cells with free Rag enhanced anti-DNP antibody response to DNP-unrelated carrier conjugate (DNP-KLH) but suppressed the response of the same cell suspension to DNP-Asc. It was also found that both IgG and IgE antibody responses to DNP-Rag were suppressed by the addition of free Asc with the homologous antigen. Mechanisms involved in the enhancement and suppression of antibody response by free carrier were explored with cell-free supernatant (CFS) obtained from the culture of primed lymph node cells with free carrier. The results showed that essentially all CFS preparations studied enhanced antibody response when they were added to primed lymph node cells which had been treated with a hapten-protein conjugate. ...
B lymphocytes with receptors specific for a particular hapten have been prepared through an antigen-affinity procedure. Methods have been developed for the clonal stimulation of these cellsin vitro, with a single, hapten-specific B cell as the unequivocal target. Many stimulatory combinations involve multivalent antigen and one or more antigen non-specific, non-MHC restricted T lymphocyte-derived growth and differentiation factors. These factors, of which there are at least 4 or 5, are progressively being defined and should soon become available through recombinant DNA technology. Judicious use of factor combinations and selected antigens should soon answer whether
Hapten Sciences is a venture-backed, start-up company developing novel new chemical entity where are no similar products in development or on the market. Clementi Ltd. works with Hapten on regulatory affairs issues and clinical development plans. Not only does Hapten rely on Clementi and Associates for these critical areas, but also on broad strategic and cross-functional development issues and opportunities. After having worked together for four years, Clementi and Associates Ltd is regarded by Hapten and the company partners as the most trusted and relied upon advisory group we have had.. ...
A hapten is a small molecule that can stimulate an strong immune response only when it is in combination with a larger molecule such as a protein. Functionally , a hapten is virtually the equivalent of en epitope. ...
Fluorescent conjugates of several of these anti-dye and anti-hapten antibodies are also available. These antibodies can be used for direct detection of labeled phospholipids via fluorescence quenching.
Hapten: A small molecule that acts antigenic once it has been combined with carrier molecules (to form a hapten-carrier conjugate ...
neoarsphenamine: was MH 1963-92; NEOARSENOBENZOL & NOVARSENOBENZENE were see NEOARSPHENAMINE 1976-92; use ARSENICALS to search NEOARSPHENAMINE 1966-92; very toxic former antisyphilitic agent still used occasionally for infections, including those in animals; it produces skin sensitization & has become a tool in the study of immunologic tolerance as a hapten
There are 2 six-letter words containing A, H, N, P and T: HAPTEN & HATPIN. Every word on this site can be used while playing scrabble. Create other lists, that start with or end with letters of your choice.
The synthetic method for haptens of organophosphorus (OP) pesticides with a spacer arm (amino carboxylic acid) attached at the pesticide thiophosphate group was simplified to a large extent. While the previous synthetic approach for this type of haptens requires seven steps, the present process involves only two steps. Using this process, five haptens of fenthion differing in spacer arm length (4-8 atoms) were synthesized and they were conjugated to bovine serum albumin and keyhole limpet hemocyanin to be used as immunogens. Rabbits were immunized with these hapten-protein conjugates for production of polyclonal antibodies against fenthion. The five haptens were conjugated to ovalbumin to be used as plate-coating antigens and twenty polyclonal antisera to the haptens were screened against each of the five coating antigens using noncompetitive and competitive indirect enzyme-linked immunosorbent assay (ELISA). The titer difference between the homologous and heterologous combinations was small, suggesting
Control of small molecule hapten epitope densities on antigenic carrier proteins is essential for development and testing of optimal conditions for vaccines. Yet, accurate determination of epitope density can be extremely difficult to accomplish, especially with the use of small haptens, large molecular weight carrier proteins, and limited amounts of protein. Here we report a simple radiometric method that uses 14C-labeled cystine to measure hapten epitope densities during sulfhydryl conjugation of haptens to maleimide activated carrier proteins.
CYTOTOXISCHE T-ZELLEN + KILLER T-ZELLEN (IMMUNOLOGIE); HAPTENE (IMMUNOLOGIE); KLONIEREN + KLONALKULTUREN + INZUCHT (BIOLOGISCHE TECHNIKEN); CYTOTOXIC T CELLS + KILLER T CELLS (IMMUNOLOGY); HAPTENS (IMMUNOLOGY); CLONING + CLONAL CULTURES + INBREEDING (BIOLOGICAL TECHNIQUES ...
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So, in Mitochondria Pt. 2 I threw a whole lot of information at you, some of it without much context. In this post, I will hopefully provide some of that context. Specifically, we will explore the the different types of electron carrier molecules that are present in the electron transport chain. Most electron carrier molecules…
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Smith L.M, J. Parce W, Smith BA, McConnell HM. 1979. Antibodies bound to lipid haptens in model membranes diffuse as rapidly as the lipids themselves. Proceedings of the National Academy of Sciences of the United States of America. 76:4177-9. ...
Smith L.M, J. Parce W, Smith BA, McConnell HM. 1979. Antibodies bound to lipid haptens in model membranes diffuse as rapidly as the lipids themselves. Proceedings of the National Academy of Sciences of the United States of America. 76:4177-9. ...
View Notes - Slides3 011911(1) from BIOL 212 at UPenn. Today: 1) Short discussion of ATP and other carrier molecules 2) Protein folding 3) Exploration of adenylate kinase structure and
Adars Maleimide-Activated KLH will simplify the production of hapten - carrier conjugates for immunizations by crosslinking the activated KLH to cysteine - containing peptides through thiol groups ...
Keywords: Three-dimensional structure of antibodies & their complexes with haptens & antigens. Subject area(s): Immunology , Structural Biology & Biophysics. ...
koteswararao2kanugula at gmail.com wrote: , Dear sir, , This is koteswara rao , I am facing the problem of , coupling reaction as well as identification of the hapten + protien , coupling. Both Molecular Probes (www.probes.com) and Pierce (www.piercenet.com) discuss cross-linkers in their respective catalogues, which are available online ...
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If you are still wondering on how to lose weight and tired of hearing nasty opinions from other people, then Chiropractic treatment is the answer to t...
Allergic contact dermatitis (ACD) is a common T cell mediated skin disease. Small reactive organic molecules called haptens induce ACD. Haptens are not recognized by themselves, but need to bond to endogenous proteins in the skin, and processed further into complete antigens. Antigen specific T cells then recognize the resulting antigen. Although the haptens are well known, the chemical reactions of haptens inducing ACD is not well known. Neither are the chemical structures of the complete antigens associated with ACD in humans. This study is composed of three parts. Reactivity study of strong sensitizers like 2,4-dinitrofluorobenzene (DNFB), 1,4-benzoquinone (BQ), 4-t-butyl-1,2-benzoquinone (tBuBQ) and cis-1,2-hexahydrophthalic-anhydride (HHPA) under physiologic conditions with nucleophilic amino acids and peptides by analytical methods (HPLC, LC/MS/MS). The new reaction products were isolated and their structures were determined by NMR and MS. We found that most haptens reacted predominantly ...
Looking for online definition of cytolipin Hcytolipin K in the Medical Dictionary? cytolipin Hcytolipin K explanation free. What is cytolipin Hcytolipin K? Meaning of cytolipin Hcytolipin K medical term. What does cytolipin Hcytolipin K mean?
Nitrophenyl (NP)-specific helper cells and suppressor cells were induced in vitro using NP-T4 bacteriophage as antigen. These cells could mediate their effects also by secreted effector molecules, helper and suppressor factors. The function of both NP-specific helper and suppressor cells was abolished by treatment with anti-Thy1.2 plus C, but they were not retained on nylon wool columns, suggesting that NP-specific helpers and suppressors were T cells. The membrane phenotype of both NP-specific helper and suppressor cells was found to be Ly1+2+I-J+(I-A-). The secreted effector molecules, helper and suppressor factors which mediate helper or suppressor function, bound to NP immunoadsorbents and are NP-specific in their function. They do not have conventional Ig determinants, but both bear determinants coded by the I-J subregion of H-2. The unusual phenotype of NP-specific helper and suppressor cells is discussed, as is the potential use of these hapten-specific T cells and their secreted effector
TY - JOUR. T1 - Hapten design and development of an ELISA (Enzyme-linked Immunosorbent Assay) for the detection of the mercapturic acid conjugates of naphthalene. AU - Marco, Maria Pilar. AU - Hammock, Bruce D.. AU - Kurth, Mark J.. PY - 1993. Y1 - 1993. N2 - Measurement of urinary metabolites constitutes a noninvasive method to assess toxic exposure. Naphthalene is a common environmental contaminant showing selective pulmonary toxicity in mice and presumably is involved in development of lung disease in man (Buckpitt, A.R.; Franklin, R.B. Pharmacol. Ther. 1989, 41, 339). A glutathione-based detoxification pathway leads to the formation of the mercapturic acid conjugates [NaphMA 1, (R)-N-acetyl-S-[(1R*,2R*)-2,2-dihydro-1-hydroxy-2-naphthyl]cysteine and (R)-N-acetyl-S-[(1R*,2R*)-1,2-dihydro-2-hydroxy-1-naphthyl]cysteine] which are excreted in urine. Herein we report the development of an immunoassay for the specific detection of these urinary metabolites. This study confirms the importance of ...
Recombinant hamster monoclonal antibody raised against 4-hydroxy-3-nitrophenyl acetyl (NP) hapten. Original antibody is raised against hapten-protein conjugated NP12-chicken gammaglobulin. (RAB00080) - Products - Abnova
Recombinant mouse monoclonal antibody raised against 4-hydroxy-3-nitrophenyl acetyl (NP) hapten. Original antibody is raised against hapten-protein conjugated NP12-chicken gammaglobulin. (RAB00064) - Products - Abnova
Incubation of unprimed spleen B cells with high concentrations of hapten-conjugates resulted in the induction of specific unresponsiveness or tolerance to a subsequent encounter with the hapten on a potentially immunogenic carrier. This process of tolerance induction could occur in the absence of extracellular calcium. In contrast B-cell activation to both proliferation and subsequent antibody secretion is known to be calcium dependent. This means that either (1) the decisions which determine immunity and tolerance in B cells are mediated through totally distinct signalling pathways, or that (2) if tolerance and immunity depend on same common signalling events, then the commitment of B cells to switch on or off must be determined at a very early stage.
T helper cell factors (HF) have been preparated against protein and synthetic antigens by restimulating in vitro induced helper cells with small amounts of antigen. HF when tested in vitro are antigen specific and capable of replacing T cells, initiating a thymus dependent IgM response. In the in vivo adoptive transfer assay HF is capable of replacing T helper cells and is active at very high dilution, inducing both IgM and IgG responses. When tested on unirradiated DNP primed animals the HF was able to initiate a thymus dependent anti-hapten response, comparable to that seen with helper T cells, of both IgM and IgG class. It was also shown that HF acts on anti-Thy 1 treated spleen cells. The results indicate that antigen specific helper factors may be one of the physiologic mediators of T-B interactions in intact animals.
To study the distribution of glycine immunoreactive neurons in the spinal cord and brain, antisera were raised against glycine conjugated to protein carriers. High-titer rabbit glycine antiserum was purified by affinity chromatography. Testing against other amino acids and peptides with immuno dot blots and ELISA assays showed little apparent cross-reaction with glutamate, aspartate, glutamine, taurine, and 17 other amino acids and related compounds. Similarly, the antiserum showed little apparent recognition of glycine when glycine was incorporated into peptides. A slight cross-reactivity with GABA, beta- alanine, and cysteine was found. Immunocytochemical labeling of tissue sections could be blocked with glycine conjugated to a heterologous carrier protein but not by other amino acids conjugated to that protein. Immunocytochemistry at the light microscope level with immunofluorescence and silver-intensified colloidal gold revealed a wide distribution of glycine-like immunoreactivity throughout ...
TY - JOUR. T1 - Catalytic antibodies. AU - Benkovic, Stephen. PY - 1992/1/1. Y1 - 1992/1/1. UR - http://www.scopus.com/inward/record.url?scp=0026717963&partnerID=8YFLogxK. UR - http://www.scopus.com/inward/citedby.url?scp=0026717963&partnerID=8YFLogxK. U2 - 10.1146/annurev.bi.61.070192.000333. DO - 10.1146/annurev.bi.61.070192.000333. M3 - Review article. C2 - 1497313. AN - SCOPUS:0026717963. VL - 61. SP - 29. EP - 54. JO - Annual Review of Biochemistry. JF - Annual Review of Biochemistry. SN - 0066-4154. ER - ...
IMMUNOLOGICAL MEASUREMENT METHOD USING HAPTEN AND ANTIBODY BINDING THERETO AS REFERENCE ANTIBODY, AND... | METHODS FOR DETECTING TRAUMATIC BRAIN INJURY | PERSONALIZED MEDICINE APPROACH FOR TREATING COGNITIVE LOSS | METHODS FOR DETERMINING DIFFERENCES IN ALPHA-4 INTEGRIN ACTIVITY BY CORRELATING DIFFERENCES IN sVCAM AND/OR... | SYSTEM AND METHOD FOR STUDYING MATRIX VESICLE CALCIFICATION |
A couple of quick thoughts: When you make a MAb hybridoma, you are doubling the amount of chromosomes in the cell, and the cell wishes to become diploid once again. Hence, it degrades or spits out the chromosomes it doesnt need (in this case, the genetic info needed to produce your antibody). 1) Try a different immunization protocol. You may need to immunize longer and more frequently to get a higher titer. 2) I do not know how you are attaching the hapten to the carrier protein. There may be insufficient epitope present to generate the specific response desired, and the mouse is reacting more to your carrier rather than to your epitope. By increasing the target immunogen on the carrier (if possible) you may get a better response. 3) You may want to use an adjuvant of some type to boost the response ...
TY - JOUR. T1 - Properties of a single-chain antibody containing different linker peptides. AU - Alfthan, Kaija. AU - Takkinen, Kristiina. AU - Sizmann, Dorothea. AU - Söderlund, Hans. AU - Teeri, Tuula. N1 - Project code: BEL2026. PY - 1995. Y1 - 1995. N2 - Single-chain antibodies were constructed using six different linker peptides to join the VH and VL domains of an anti-2-phenyloxazolone (Ox) antibody. Four of the linker peptides originated from the interdomain linker region of the fungal cellulase CBHI and consisted of 28, 11, six and two amino acid residues. The two other linker peptides used were the (GGGGS)3 linker with 15 amino acid residues and a modified IgG2b hinge peptide with 22 residues. Proteolytic stability and Ox binding properties of the six different scFv derivatives produced in Escherichia coli were investigated and compared with those of the corresponding Fv fragment containing no joining peptide between the V domains. The hapten binding properties of different antibody ...
TY - JOUR. T1 - The antitumor monoclonal antibody MOv2 recognizes the Lewis A hapten. AU - Leoni, F.. AU - Magnani, J. L.. AU - Miotti, S.. AU - Canevari, S.. AU - Pasquali, M.. AU - Sonnino, S.. AU - Colnaghi, M. I.. PY - 1988. Y1 - 1988. N2 - Monoclonal antibody MOv2, produced against ovarian carcinoma, was previously found to bind a carbohydrate epitope (CAMOv2) present on mucins, glycoproteins and a neutral glycolipid. In this paper, the structure of the carbohydrate epitope is determined by immunological reactivity with purified glycolipids and oligosaccharides. Using solid-phase radioimmunoassay and immunostaining of thin layer chromatograms, MOv2 binds strongly to Le(a)-active pentasaccharide ceramide. A smaller neutral glycolipid also weakly binds MOv2. Fifty percent inhibition of binding to Le(a)-active pentasaccharide ceramide is achieved with approximately 8 μM concentration of lacto-N-fucopentaose II (LNF II). Lacto-N-tetraose (LNT) also partially inhibits at about 103 times higher ...
The number of deaths due to heroin overdoses has severely increased in last decade, and regular consumption of heroin leads to serious health concerns.. Vaccination is one of the emerging choices among heroin rehabilitation therapies. In general, these vaccines contain heroin-like hapten as a pharmacophore that acts as an antagonist. This antagonist has high affinity for heroin receptors but does not activate them, hence reverses the effects of heroin. Because of inherent chemical instability, when entered in to bloodstream these anti-heroin drugs rapidly metabolise into several by-products, which makes development of effective vaccines against heroin particularly challenging.. Recently an American research group at National Institute on Drug Abuse (NIDA) lead by Professor Kenner Rice has reported the synthesis and immunological evaluation for a series of haptens.. The team has emphasised the hydrolytic stability of hapten scaffolds and envisioned that hapten with amido groups at both the C3 and ...
The p-i concept is not conceived as opposing the hapten or prohapten concept, but supplementing it. Certain drugs such as penicillins may cause hypersensitivity reactions caused by hapten-carrier formation. Others, such as quinolones and sulfanilamides, may cause hypersensitivity by the hapten and p-i concept simultaneously [4, 22, 24]. Thus, if the hapten concept is well proven for a certain drug such as p-phenylenediamine or SMX, it does not rule out that the p-i concept is not also playing some role.. The p-i concept is contradicting many well-established rules in immunology. However, this is no argument against it as it is no more immunology but pharmacology; and the findings in drug allergy do contradict many established rules [35]. Actually, the fact that the p-i concept is not implying an immune response to a drug may explain many of these puzzling findings. Drug hypersensitivity generated according to the p-i concept is not caused by a newly generated immune response to the drug but is ...
Previous work established that a TLR ligand attached to a haptenated protein Ag or contained within a virus particle can promote the magnitude and quality of the GC reaction and, moreover, that TLR signaling within the Ag-specific B cell is an important contributor to that enhancement (7, 13). In this study, we used mRNA sequencing and GSEA to identify the intracellular transcriptional programs that were enhanced by coupled Ag and TLR9/MyD88 signaling in GC B cells. Especially enhanced were the transcriptional signatures associated with c-Myc transcriptional activity and mTORC1 signaling. Both of these pathways are central to the control of cell growth and proliferation in many cell types and are critical in B cells for the GC response (17, 32, 33). Interestingly, Tfh cells recognizing Ag presented by GC B cells also stimulate these pathways in the Ag-presenting GC B cell, leading to positive selection of the GC B cells (18, 19, 37). Thus, one potential mechanism by which TLR9/MyD88 signaling ...
LLPC may also be used to detect conformational changes occuring upon binding of antigen by antibody. Antigen-antibody complexes formed by different lgG antibodies against a large antigen like HSA all had similar surface properties. different from those of both antigen and antibody. In contrast, the surface properties of complexes formed by small antigens haptens are related to those of the lgG antibody ...
0035] It will be understood that the haptens of the current invention may be attached to the antigenicity-conferring carrier material (accm) via a crosslinking group. The crosslinking group may be any conventional cross linking group conventionally used in this field. The crosslinking group is ideally a functionalised linking group joining the accm to the hapten. The term "crosslinking group" as used herein is any bifunctional molecule able to covalently join the hapten element to an immunogenicity conferring carrier material. A suitable crosslinking group to link with alternative carrier materials is maleimide, or a maleimide derivative, for example when BTG-maleimide is used to conjugate with the hapten via a cysteine residue. Other cross-linking groups which could also couple this group on the cysteine include haloacetyls and pyridyldisulfides. Either Lys residue, or the Glu residue (C-terminal) may alternatively be used to conjugate to a carrier material, optionally via a cross-linking ...