Define Haemophilus influenzae type b. Haemophilus influenzae type b synonyms, Haemophilus influenzae type b pronunciation, Haemophilus influenzae type b translation, English dictionary definition of Haemophilus influenzae type b. n. Abbr. Hib A rod-shaped, gram-negative bacterium found in the human respiratory tract that causes acute respiratory infections, such as pneumonia, and...
Description of disease Vaccination, Haemophilus influenzae type B (HIB). Treatment Vaccination, Haemophilus influenzae type B (HIB). Symptoms and causes Vaccination, Haemophilus influenzae type B (HIB) Prophylaxis Vaccination, Haemophilus influenzae type B (HIB)
Non-typeable Haemophilus influenzae (NTHi) and encapsulated Haemophilus influenzae type b (Hib) were inoculated into the middle ears of Sprague-Dawley rats. Tympanic membrane (TM) status was assessed otomicroscopically and specimens from various middle ear areas were prepared for light microscopy at various times during the acute phase and up to 6 months after inoculation. Irrespective of bacteria strain, acute otitis media (AOM) was present in all ears 4 days after inoculation. The Hib-infected ears showed initially a severe course of AOM, but all were otomicroscopically resolved by day 12, at which time a few NTHi-inoculated ears still exhibited middle ear effusion. The TMs infected with Hib had normalized without scar formation, whereas NTHi induced a persistent thickening of the TMs in half of all cases. The middle ear mucosa of NTHi-infected ears initially showed vigorous activity among the goblet cells, but the mucosa normalized after the acute phase. Hib, by contrast, induced prominent ...
article{fc3e4d3f-eec5-4f6b-8f7e-bed105dc5dde, abstract = {,p,Non-typable Haemophilus influenzae (NTHi) are small, gram-negative bacteria and are strictly human pathogens, causing acute otitis media, sinusitis and community-acquired pneumonia. There is no vaccine available for NTHi, as there is for H. influenzae type b. Recent advances in proteomic techniques are finding novel applications in the field of vaccinology. There are several protein separation techniques available today, each with inherent advantages and disadvantages. We employed a combined proteomics approach, including sequential extraction and analytical two-dimensional polyacrylamide electrophoresis (2D PAGE), and two-dimensional semi-preparative electrophoresis (2D PE), in order to study protein expression in the A4 NTHi strain. Although putative vaccine candidates were identified with both techniques, 11 of 15 proteins identified using the 2D PE approach were not identified by 2D PAGE, demonstrating the complementarily of the ...
TY - JOUR. T1 - Hemophilus influenzae pharyngitis and cellulitis in adults.. AU - Kroneman, O. C.. AU - Brody, H.. PY - 1980/11/1. Y1 - 1980/11/1. N2 - Hemophilus influenzae infections in adults are becoming more common but are often unsuspected in this age group by the primary care physician. Two case reports illustrate pharyngitis, and pharyngitis associated with cellulitis of the neck, in which H influenzae was cultured from the blood. The throat and skin are only two of the many sites for H influenzae infections in adults. As no physical signs are pathognomonic for this organism, its possible role should influence the choice of antibiotics while awaiting culture results. Newer cephalosporins, especially cefamandole and cefoxitin, appear promising in the treatment of these infections.. AB - Hemophilus influenzae infections in adults are becoming more common but are often unsuspected in this age group by the primary care physician. Two case reports illustrate pharyngitis, and pharyngitis ...
Haemophilus influenzae (including Hib) is a bacterium that can cause a severe infection, occurring mostly in infants and children younger than five years of age. It can cause lifelong disability and be deadly. In spite of its name, Haemophilus influenzae bacteria do not cause influenza (the flu).. There are six identifiable types of Haemophilus influenzae bacteria (a through f) and other non-identifiable types (called nontypeable). The one most people are familiar with is Haemophilus influenzae type b, or Hib. Theres a vaccine that can prevent disease caused by Hib, but not the other types of Haemophilus influenzae bacteria.. ...
Haemophilus influenzae type b HAEMOPHILUS INFLUENZAE TYPE B (HIB) VACCINES FOR AUSTRALIAN CHILDREN: INFORMATION FOR IMMUNISATION PROVIDERS Disease and epidemiology Haemophilus influenzae type b (Hib) is
Example(s): ACT-HIB (Haemophilus influenzae type b (Hib) vaccine),Infanrix Hexa (tetanus, diphtheria, pertussis, polio, Haemophilus influenzae type b, and hepatitis B combined vaccine (DTaP-IPV-Hib-hep B, called the 6-in-1)),Pentacel (tetanus, diphtheria, pertussis, polio, and Haemophilus influenzae type b combined vaccine (DTaP-IPV-Hib, called the 5-in-1))
Background: The rate of Haemophilus influenzae type b (HIB) infections has decreased dramatically since the use of HIB vaccines in infants and children. The current prevalence of invasive HIB infections and those due to non-type b H. influenzae is not fully known. The objective was to describe the cases of all invasive H. influenzae infections and describe the spectrum and severity of clinical disease. Methods: Retrospective study of all hospitalized patients with culture-proven invasive Haemophilus influenzae infections at Nationwide Childrens Hospital, Columbus, Ohio, from 2009 to 2018. The electronic health records were reviewed for pertinent demographic, clinical, laboratory data, and outcomes. Results: There were a total of 59 culture-proven H. influenzae infections of which 12 were excluded due to insufficient patient data. The remaining 47 patients (32 [68%] male; 30 [64%] white, 8 [17%] African-American) and their culture results are provided in Table ...
Our investigation of a large collection of invasive NTHi isolates has allowed us to determine if particular LOS biosynthetic genes are present and have altered expression in sterile niches in the human host. While five out of seven of these biosynthetic genes (lic1A, lic3A, lic3B, lex2A, and lgtC) show no significant correlation with an ON or OFF expression state during invasive infection, we demonstrate that lic2A remains OFF in invasive isolates and oafA ON is statistically overrepresented in invasive isolates compared to the level in carriage isolates.. Our observation that lic2A is OFF in most invasive isolates is intriguing, as this finding appears contradictory to earlier results. Expression of lic2A was previously demonstrated to confer resistance to human serum (31), and modification of the NTHi LOS inner core with a galactose by Lic2A has been shown to shield the cells from in vitro neutrophil-mediated killing assays when lic1A is phase-varied OFF, with this modification being ...
TY - JOUR. T1 - Animal Models for the Study of Noninvasive Haemophilus influenzae Disease. T2 - Pulmonary Clearance Systems. AU - Hansen, Eric J.. AU - Toews, Galen B.. PY - 1992/6. Y1 - 1992/6. N2 - Nontypeable Haemophilus influenzae are increasingly being recognized as an important cause of disease in the lower respiratory tract. Information about the pathogenesis of these infections has been limited until recently by the paucity of experimental animal models for studying the host-parasite interaction in vivo. Three different animal models for investigation of the interaction of nontypeable H. influenzae with the lower respiratory tract have been used: Two involve the evaluation of short-term pulmonary clearance in mice or rats, while a new, long-term infection model in rats mimics the more chronic bronchopulmonary infections sometimes caused by these organisms. Results obtained in these three model systems indicate that both humoral and cellular immune mechanisms are involved in immunity to ...
PubMedID: 23291945 | Safety, reactogenicity and immunogenicity of a booster dose of the 10-valent pneumococcal non-typeable Haemophilus influenzae protein D conjugate vaccine (PHiD-CV) in Malian children. | Human vaccines & immunotherapeutics | 2/1/2013
Acute otitis media (AOM) is one of the most frequently encountered bacterial infections in children aged | 5 years; Streptococcus pneumoniae (S. pneumoniae) and non-typeable Haemophilus influenzae (NTHi) are historically identified as primary AOM causes. Nevertheless, recent data on bacterial pathogens causing AOM in Latin America are limited. This prospective study aimed to identify and characterize bacterial etiology and serotypes of AOM cases including antimicrobial susceptibility in | 5 year old Colombian children. From February 2008 to January 2009, children ≥3 months and | 5 years of age presenting with AOM and for whom a middle ear fluid (MEF) sample was available were enrolled in two medical centers in Cali, Colombia. MEF samples were collected either by tympanocentesis procedure or spontaneous otorrhea swab sampling. Bacteria were identified using standard laboratory methods, and antimicrobial resistance testing was performed based on the 2009 Clinical and Laboratory Standards Institute (CLSI
TY - JOUR. T1 - Structural and antigenic conservation of the P2 porin protein among strains of Haemophilus influenzae type b. AU - Hansen, E. J.. AU - Pelzel, S. E.. AU - Orth, K.. AU - Moomaw, C. R.. AU - Radolf, J. D.. AU - Slaughter, C. A.. PY - 1989. Y1 - 1989. N2 - The P2 porin protein is the most abundant protein in the outer membrane of Haemophilus influenzae type b (Hib). Biochemical and immunochemical techniques were used to characterize the P2 proteins from a number of different Hib strains. P2 proteins from Hib outer membrane vesicles were resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred to nitrocellulose for in situ tryptic digestion. Solid-phase tryptic digests of P2 from eight Hib strains were resolved by high-pressure liquid chromatography and shown to be similar if not identical. Radioimmunoprecipitation analysis involving Hib cells (containing intrinsically radiolabeled proteins or lipooligosaccharide) and Western blot (immunoblot) analysis ...
TY - JOUR. T1 - Synergistic activation of NF-κB by nontypeable Haemophilus influenzae and tumor necrosis factor α. AU - Watanabe, Takahiro. AU - Jono, Hirofumi. AU - Han, Jiahuai. AU - Lim, David J.. AU - Li, Jian Dong. PY - 2004/3/9. Y1 - 2004/3/9. N2 - Nontypeable Haemophilus influenzae (NTHi) is an important human pathogen causing otitis media in children and exacerbation of chronic obstructive pulmonary disease in adults. Like most other bacterial infections, NTHi infections are also characterized by inflammation, which is mainly mediated by cytokines and chemokines such as tumor necrosis factor α (TNF-α). Among a variety of transcription regulators, NF-κB has been shown to play a critical role in regulating the expression of large numbers of genes encoding inflammatory mediators. In review of the current studies on NF-κB regulation, most of them have focused on investigating how NF-κB is activated by a single inducer at a time. However, in bacteria-induced inflammation in vivo, ...
Despite nearly complete vaccine coverage, a small number of fully vaccinated children in the Netherlands have experienced invasive disease caused by Haemophilus influenzae serotype b (Hib). This increase started in 2002, nine years after the introduction of nationwide vaccination in the Netherlands. The capsular polysaccharide of Hib is used as a conjugate vaccine to protect against Hib disease. To evaluate the possible rise of escape variants, explaining the increased number of vaccine failures we analyzed the composition of the capsular genes and the expressed polysaccharide of Dutch Hib strains collected before and after the introduction of Hib vaccination. The DNA sequences of the complete capsular gene clusters of 9 Dutch Hib strains were assessed and two variants, designated type I and type II were found. The two variants displayed considerable sequence divergence in the hcsA and hcsB genes, involved in transport of capsular polysaccharide to the cell surface. Application of hcsA type specific
Uncurated}} [[Image:Disease_hib2.jpg,frame,thumbnail,50px,alt=Culture of ,i>Haemophilus influenzae,/i> on blood agar plate.,Culture of ,i>Haemophilus influenzae,/i> [21]]] [[image:swollen_foot.jpg,frame,Affected individual with swollen foot [21] ]] [[image:child_swolllen.jpg,frame,Child affected with face swollen [21] ]] ==Introduction== ,i>Haemophilus influenzae,/i> was first described by Richard Pfeiffer in 1982 during the outbreak of influenza. The name of this bacterium was given by Winslow, et al. in 1920. [3] ,i>Haemophilus influenzae,/i> was the leading cause of death worldwide before the introduction of an effective vaccine in 1990s. It is the main cause of bacterial meningitis in many developing countries. ,i>H.influenzae,/i>. characterizes a group of bacteria that may cause different types of clinical syndromes in infants and children under the age of five. H. influenzae can cause ear, eye, or sinus infections, pneumonia, meningitis, and a life threatening infection called ...
TY - JOUR. T1 - Regulation of virulence gene expression resulting from streptococcus pneumoniae and nontypeable haemophilus influenzae interactions in chronic disease. AU - Cope, Emily K.. AU - Goldstein-Daruech, Natalia. AU - Kofonow, Jennifer M.. AU - Christensen, Lanette. AU - McDermott, Bridget. AU - Monroy, Fernando. AU - Palmer, James N.. AU - Chiu, Alexander G. AU - Shirtliff, Mark E.. AU - Cohen, Noam A.. AU - Leid, Jeff G.. PY - 2011/12/5. Y1 - 2011/12/5. N2 - Chronic rhinosinusitis (CRS) is a common inflammatory disease of the sinonasal cavity mediated, in part, by polymicrobial communities of bacteria. Recent molecular studies have confirmed the importance of Streptococcus pneumoniae and nontypeable Haemophilus influenzae (NTHi) in CRS. Here, we hypothesize that interaction between S. pneumoniae and NTHi mixed-species communities cause a change in bacterial virulence gene expression. We examined CRS as a model human disease to validate these polymicrobial interactions. Clinical ...
The Report Haemophilus influenzae Type B Infections Global Clinical Trials Review, H1, 2016 provides information on pricing, market analysis, shares,...
H. influenzae type b has been nearly abolished in the U.S. due to effective vaccine development, which has been available since 1988. Immunization with the Hib vaccine can help prevent Haemophilus influenzae type b disease. In rare cases, children may still develop H. influenzae type b infections. This can occur if the child has not completed their series of immunizations or in older children who did not receive the vaccine as an infant. ...
H. influenzae type b has been nearly abolished in the U.S. due to effective vaccine development, which has been available since 1988. Immunization with the Hib vaccine can help prevent Haemophilus influenzae type b disease. In rare cases, children may still develop H. influenzae type b infections. This can occur if the child has not completed their series of immunizations or in older children who did not receive the vaccine as an infant.. ...
TY - JOUR. T1 - Significance of tagI and mfd genes in the virulence of non-typeable Haemophilus influenzae. AU - Spricigo, Denis A.. AU - Cortes, Pilar. AU - Moranta, David. AU - Barbe, Jordi. AU - Bengoechea, Jose Antonio. AU - Llagostera, Montserrat. PY - 2014. Y1 - 2014. N2 - Non-typeable Haemophilus influenzae (NTHi) is an opportunist pathogen well adapted to the human upper respiratory tract and responsible for many respiratory diseases. In the human airway, NTHi is exposed to pollutants, such as alkylating agents, that damage its DNA. In this study, we examined the significance of genes involved in the repair of DNA alkylation damage in NTHi virulence. Two knockout mutants, tagI and mfd, encoding N(3)methyladenine-DNA glycosylase I and the key protein involved in transcription-coupled repair, respectively, were constructed and their virulence in a BALB/c mice model was examined. This work shows that N-3-methyladenine-DNA glycosylase I is constitutively expressed in NTHi and that it is ...
Collected resources and information for haemophilus influenzae type b (hib) disease and vaccination: Access the latest recommendations, news, information, and resources from IAC, government agencies, professional journals, and other organizations in one spot on immunize.org.
Non-typeable Haemophihis influenzae (NTHi) is a significant cause of otitis media in children. We have employed single and multiple step electrospray ionization mass spectrometry (ESIMS) and NMR spectroscopy to profile and elucidate lipopolysaccharide (LPS) structural types expressed by NTHi strain 162, a strain obtained from an epidemiological study in Finland. ESIMS on O-deacylated LPS (LPS-OH) and core oligosaccharide (OS) samples of LPS provided information on the composition and relative abundance of glycoforms differing in the number of hexoses linked to the conserved inner-core element, L-alpha-D-Hepp-(1--, 2)-[PEtn --, 6]-L-alpha-D-Hepp-(1 --, 3)-L-alpha-D-Hepp-(1 --, 5)(.)[PPEtn --, 4]-alpha-Kdop-(2 --, 6)-Lipid A of H. influenzae LPS. The strain examined was found to elaborate Hex2 to Hex5 LPS glycoform populations having structures identical to those observed for H. influenzae strain Rd [Risberg, A.; Masoud, H.; Martin, A.; Richards, J.C.; Moxon, E.R.; Schweda, E.K.H. Eur. J Biochem. ...
Haemophilus influenzae is gram negative rod. There are 6 serotypes of haemophilus influenzae which include serotypes a, b,c, d, e and f which are based on the polysaccharide capsules.
The chemical structure and biologic function of the lipid A portion of lipopolysaccharide are not identical among gram-negative bacteria. This study indicates that antigenically heterogeneous lipid A exists among strains of Haemophilus influenzae. An immunoglobulin G3 murine monoclonal antibody, 3D2, produced against a nontypable H. influenzae strain 3524 has specificity for a site on the lipid A portion of the H. influenzae lipopolysaccharide. With the Western blot and immunodot assay, 3D2 recognized this lipid A determinant on 14 of 24 (58%) of strains of nontypable H. influenzae and in 51 of 95 (54%) strains of H. influenzae type b. This lipid A epitope has a high degree of specificity for H. influenzae, since it is not present on the lipid A of 39 gram-negative strains from 14 non-Haemophilus species. In addition, studies of 36 strains of six Haemophilus species other than H. influenzae and 8 strains of 4 species of Actinobacillus did not contain the 3D2 epitope. Enzyme-linked immunosorbent ...
Haemophilus influenzae (H. influenzae) is a group of bacteria that can cause different types of infections in babies and children. H. influenzae most often cause ear, eye, or sinus infections. They also cause pneumonia.
article{a2ab6767-ca6c-4527-8f1e-217da06cea72, abstract = {Nontypeable Haemophilus influenzae (NTHi) commonly causes local disease in the upper and lower respiratory tract and has recently been shown to interfere with both the classical and alternative pathways of complement activation. The terminal pathway of the complement system is regulated by vitronectin that is a component of both plasma and the extracellular matrix. In this study, we identify protein E (PE; 16 kDa), which is a recently characterized ubiquitous outer membrane protein, as a vitronectin-binding protein of NTHi. A PE-deficient NTHi mutant had a markedly reduced survival in serum compared with the PE-expressing isogenic NTHi wild type. Moreover, the PE-deficient mutant showed a significantly decreased binding to both soluble and immobilized vitronectin. In parallel, PE-expressing Escherichia coli bound soluble vitronectin and adhered to immobilized vitronectin compared with controls. Surface plasmon resonance technology ...
Haemophilus influenzae is caused by bacteria which strikes children younger than 5 years old. So book a visit now to get a haemophilus influenzae type b vaccine as an injection for your child.
Haemophilus influenzae are fastidious facultatively anaerobic Gram-negative bacteria that cause a range of human infections including otitis media, meningitis, epiglottitis and pneumonia [1, 2]. H. influenzae lacks all enzymes in the biosynthetic pathway for the porphyrin ring and as a result is unable to synthesize protoporphyrin IX (PPIX), the immediate precursor of heme. Since H. influenzae cannot synthesize PPIX the organism has an absolute growth requirement for an exogenous source of PPIX or heme [3, 4]. As a result of this growth requirement H. influenzae has evolved a complex multifunctional array of uptake mechanisms to ensure that it is able to utilize available porphyrin in vivo [5]. One protein shown to be involved in utilization of heme by H. influenzae is the heme binding lipoprotein HbpA [6-8]. HbpA was initially identified as a potential constituent of a heme acquisition pathway following transformation of an H. influenzae genomic DNA library into Escherichia coli and screening ...
This page describes the Haemophilus influenzae type B bacteria which causes severe infections in young children, including treatment and preventative approaches(vaccination)
TY - JOUR. T1 - Opacity-associated protein A contributes to the binding of Haemophilus influenzae to chang epithelial cells. AU - Prasadarao, Nemani V.. AU - Lysenko, Elena. AU - Wass, Carol A.. AU - Kim, Kwang Sik. AU - Weiser, Jeffrey N.. PY - 1999/8. Y1 - 1999/8. N2 - Opacity-associated protein A (OapA), which is responsible for the transparent-colony phenotype of Haemophilus influenzae, has been implicated in the colonization of the nasopharynx in an infant rat model of carriage. In this report, we show that OapA mediates attachment to Chang epithelial cells examined by using genetically defined type b and nontypeable H. influenzae strains with or without OapA. We also showed that OapA was conserved among H. influenzae strains by comparing deduced amino acid sequences. Both recombinant OapA and polyclonal anti-OapA antiserum blocked the binding of H. influenzae to Chang epithelial cells, suggesting that the interaction of H. influenzae is specific to OapA. Moreover, the binding of ...
This is a retrospective study about serotype distribution and antibiotic resistance of MEF S. pneumoniae and H. influenzae isolates collected from children before introduction of PCV10 and Hib conjugate vaccines nationwide. Microbiological diagnosis of AOM is not done on a routine basis in Bulgaria and this mild disease is underrepresented in our data set. Also, the tympanocentesis is not a common practice in our country. Thus, bacterial cultures studied here most likely represent cases of severe otitus media or patients with a complicated AOM.. The analysis included 128 S. pneumoniae isolates, of which 86% occurring in children aged ≤ 5 years. We found a relatively high rate of serotype 19F infections (20.3%), which is in accordance with the serotype distribution observed in the studies of AOM in German children [8], and the Finland [7]. Serotypes 6B and 19A were next most prominent, each comprising about 16% and 11%, respectively of the overall data set. The coverage calculations for the ...
At its meeting on April 16, 1975, the Committee on Infectious Diseases reconsidered the problem of ampicillin-resistant strains of Hemophilus influenzae type b. The following facts were noted:. (1) Strains of H. influenzae type b highly resistant in vitro to ampicillin have been reported from 20 states and the District of Columbia.1. (2) These strains were isolated from children with sepsis, meningitis, cellulitis, epiglottitis, suppurative arthritis, and pneumonia. Some of the children died when the resistance of the etiologic strain was not appreciated.. (3) The prevalence of these strains is still uncertain but appears to be at a low level in most communities. Epidemiologic studies, however, indicate these strains may infect many children in closed communities, such as day-care centers.2. The Committee believes a modification of its prior statement3 is warranted based on the wide-spread occurrence of these strains:. (1) Initial management of children with documented or suspected severe ...
Introduction to Annual Summary of Communicable Diseases, 2002. List of Reportable Diseases, 2002. Number of Cases of Selected Reportable Diseases, 2002. Fifty-six cases of invasive Haemophilus influenzae disease (1.2 per 100,000 population) were reported in 2001. Case-patients ranged in age from newborn to 89 years, (median, 63 years). Nineteen (34%) had pneumonia, 22 (39%) had bacteremia without another focus of infection, four (7%) had meningitis, and 11 (20%) had other conditions. Twelve (21%) deaths were reported among these cases.. Only one case was known to be type b (Hib), compared to three cases in 2000 and five cases in 1999. The Hib case reported in 2001 occurred in a 5-year-old with a complete vaccine history and no underlying medical conditions. Epiglottitis was the type of infection, and the patient survived. Nineteen (34%) of the Haemophilus influenzae isolates were not available for typing at MDH. Of the total 37 isolates for which typing was performed (including the one [3%] type ...
Haemophilus influenzae (tudi Pfeifferjev bacil) je vrsta hemofilusov[1], gramnegativnih pleomorfnih (polimorfnih) bacilov, to pomeni, da se njihova oblika prilagaja okolju, lahko postanejo bacili, kokobacili ali tudi vibriji. Je fakultativno anaerobna oportunistična bakterija, ki jo je prvi opisal Richard Pfeiffer leta 1892 med pandemijo gripe oziroma influence.[2] Sprva so zmotno predvidevali, da povzroča gripo, in sicer vse do leta 1933, ko so odkrili virus gripe. Haemophilus influenzae, pri 80% populacije, naseljuje nosno-žrelni predel (nasopharynx) in je del normalne mikrobne flore. Haemophilus influenzae (tipa B) povzroča različne lokalizirane in invazivne okužbe,[3] zlasti vnetja dihal, paranazalnih sinusov, srednjega ušesa, veznic, možganskih ovojnic, seroznih mren in sklepov, lahko pa tudi sepso. H. influenzae raste le na krvnem agarju in čokoladnem agarju, na katerega je cepljen tudi Staphylococcus aureus[1], s tem dodamo rastni faktor V (koencim ali NAD+) in X (hematin), pri ...
TY - JOUR. T1 - Tentative criteria for determining the in vitro susceptibilities of Haemophilus influenzae, including quality control parameters, to two fluoroquinolones (grepafloxacin and PD 131628). AU - Sewell, David L.. AU - Barry, Arthur L.. AU - Allen, Stephen D.. AU - Fuchs, Peter C.. AU - Jorgensen, James H.. AU - Tenover, Fred. PY - 1995/3. Y1 - 1995/3. N2 - Grepafloxacin (OPC 17116) and PD 131628 were evaluated against 150 Haemophilus influenzae isolates to propose susceptibility testing criteria for both broth dilution and disk diffusion procedures using haemophilus test medium. Grepafloxacin-susceptible isolates are defined as those for which minimum inhibitory concentrations (MICs) are ≤0.06 μg/ml and zones of inhibition are ≥27 mm. PD 131628-susceptible strains of H. influenzae included those that exhibited MICs ≤0.03 μg/ml, and the zones were ≥32 mm. All MICs were well below concentrations that can be achieved in the blood and tissues of patients treated with either ...
The Haemophilus influenzae HMW1 adhesin is a high-molecular weight protein that is secreted by the bacterial two-partner secretion pathway and mediates adherence to respiratory epithelium, an essential early step in the pathogenesis of H. influenzae disease. In recent work, we discovered that HMW1 is a glycoprotein and undergoes N-linked glycosylation at multiple asparagine residues with simple hexose units rather than N-acetylated hexose units, revealing an unusual N-glycosidic linkage and suggesting a new glycosyltransferase activity. Glycosylation protects HMW1 against premature degradation during the process of secretion and facilitates HMW1 tethering to the bacterial surface, a prerequisite for HMW1-mediated adherence. In the current study, we establish that the enzyme responsible for glycosylation of HMW1 is a protein called HMW1C, which is encoded by the hmw1 gene cluster and shares homology with a group of bacterial proteins that are generally associated with two-partner secretion ...
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Five tests--satellitism, synthesis of porphyrins, acid production from sucrose, beta-galactosidase activity (ONPG), and indole production--to differentiate between strains of Haemophilus influenzae and strains of V-dependent Haemophilus species were evaluated. Six per cent of strains of H influenzae were misidentified as H parainfluenzae by a test for satellitism using filter paper discs impregnated with X factor, V factor, or both, applied to Columbia Agar. None of seven nutrient agars tested grew Haemophilus species, and determined accurately the X factor requirement. Synthesis of porphyrins from delta-aminolaevulinic acid provided a reliable means of demonstrating that X factor was required. A test for the production of acid from sucrose discriminated successfully between strains of V-dependent Haemophilus species (positive) and H influenzae (negative). Most isolates were identified correctly by the ONPG test, but occasional V-dependent strains were negative and could be misidentified as H ...
Haemophilus influenzae Antibody Does this test have other names? Haemophilus influenzae type b (Hib) IgG antibody level What is this test? This test measures the amount of anti-Hib IgG immunoglobulin, or antibody, in your blood. There are many types of Haemophilus influenza bacteria. They can live in your mouth, throat, ears, and airways and dont always cause illness. H. influenza type b (Hib) one of the most serious types. It doesnt cause the flu, although it has a similar-sounding name. It causes ba...
Viral co-infection does not reduce efficacy of vaccination against nontypeable Haemophilus influenzae middle ear infection in a rat ...
Vaccination against influenza and Streptococcus pneumoniae is recommended for elderly and immunocompromised individuals. However, there is little information concerning the efficacy of vaccination in specific groups of patients. In this study, 52 patients underwent vaccination against influenza, S. pneumoniae and Haemophilus influenzae type b (Hib) as they attended hospital outpatient clinics. Serum was analysed prior to vaccination and 4-6 weeks afterwards. Antibody titres against S. pneumoniae and Hib were compared with reference values corresponding to the geometric mean titres of a healthy UK population. For influenza vaccination, haemagglutination inhibition (HI) titres were measured against three inactivated strains; a titre of , or = 1/40 was considered protective. No patient had protective titres to all three antigens prior to vaccination and 41 patients (85%) had titres , 1/40 to all 3 strains. Post vaccination only 9/48 patients (19%) achieved protective antibody titres. Resistance to ...
BACKGROUND: Infections during pregnancy confers increased risk of maternal and perinatal morbidity and mortality. However, the case for advocating Haemophilus influenzae type B (Hib) and viral Influenza vaccinations in pregnancy is still debatable.. OBJECTIVES: To assess the impact of Hib and viral Influenza vaccinations during pregnancy on maternal, neonatal and infant health outcomes compared to placebo/control.. SEARCH METHODS: We searched the Cochrane Pregnancy and Childbirth Groups Trials Register (29 January 2015) and reference lists of retrieved studies.. SELECTION CRITERIA: All randomised controlled clinical trials (including cluster-randomised trials) and quasi-randomised trials evaluating Hib or viral influenza vaccination during pregnancy compared with no vaccination or placebo.. DATA COLLECTION AND ANALYSIS: Two review authors independently assessed trials for inclusion, risk of bias and extracted data. Data were checked for accuracy.. MAIN RESULTS: Two trials were included this ...
This study evaluated the vaccination response to Haemophilus influenzae type b (Hib) in malnourished pregnant women (MN), cord blood (CB) and in infants at two and six months of age for comparison with a control group (C). Twenty-eight malnourished pregnant women and 29 pregnant controls were immunized with conjugated Act-HIB® in the third trimester of pregnancy. Blood samples were collected from all before the immunization, during labor (post immunization), and from CB. All infants were immunized with Hib vaccine according to normal vaccine schedule and sera were collected at two and six months of age. Antibody levels to polyribosylribitol phosphate (PRP) were similar for both groups. Preimmunization: MN 1.94 µg/mL, C 1.68 µg/mL; post-vaccination: MN 18.53 µg/mL and C 17.55 µg/mL; in CB from MN 14.46 µg/mL and from C 17.04 µg/mL. Infants from MN and C mothers presented respectively at two months: 5.18 µg/mL and 8.60 µg/mL and at six months: MN 3.42 µg/mL and C 2.18 µg/mL. Antibody ...
Haemophilus influenzae type b (or Hib) is a vaccine-preventable disease that can cause ear infections, pneumonia, and bacterial meningitis, especially in young children. Learn more.
Haemophilus influenzae type b is a serious bacterial disease that usually strikes children younger than 5. It is spread from person-to-person by coughing and sneezing.
Haemophilus influenzae type b is a serious bacterial disease that usually strikes children younger than 5. It is spread from person-to-person by coughing and sneezing.
Haemophilus influenzae type b is a serious bacterial disease that usually strikes children younger than 5. It is spread from person-to-person by coughing and sneezing.