Molecular Plant-Microbe Interactions 10:394-400...Continual Green-Fluorescent Protein Monitoring of Cauliflower Mosaic Virus 35S Promoter Activity in Nematode-Induced Feeding Cells in Arabidopsis thaliana...
Read this Science Presentation or Speech and over 30,000 other research documents. Introduction to Green Fluorescent Protein (gfp).. Introduction to Green Fluorescent Protein (GFP) The green fluorescent protein (GFP) obtained from the jellyfish Aequorea victoria has become one of the most widely researched proteins in biochemistry and cell biology, In early 1960s researcher named Osamu Shimomura set out to find the reason for this luminescence , after harvesting...
OV-IA82Δ113 and OV-IA82Δ116 viruses were constructed by infecting OFTu cells (in T25 flasks) at a multiplicity of infection (MOI) of 1.0 with wild type OV-IA 82 for 3 hours and subsequently transfecting the cells with 10 μg of pSVP-113LF-EGFP-113RF and pSVP-116LF-EGFP-116RF transfer vectors by standard in vivo recombination protocols [28, 29]. Transfections were carried out using Lipofectamine 2000 (Invitrogen, Carlsbad, CA, USA) according to the manufacturers instructions.. Viruses were harvested 48 h pi by scraping infected/transfected OFTu cells into sterile 15 ml conical tubes. The cell suspensions were vortexed, frozen/thawed 3 times, and then centrifuged at 1000 rpm, for 10 min at 4°C (Eppendorf Centrifuge 5810R, 15 amp version, Hamburg, Germany). The supernatant (viruses) were transferred into 2 ml cryogenic vials (Corning, NY, USA) and stored at -80°C for future use.. In order to select and purify recombinant viruses, limited dilution and plaque assays were performed. 3 ×104 of ...
The monoclonal Anti-GFP-HRP antibody facilitates the fast and convenient detection of GFP and GFP derivatives or GFP-tagged fusion proteins in Western blot or ELISA applications. Conjugation to horseradish peroxidase (HRP) simplifies Western blot or ELISA analysis as incubation with secondary antibodies becomes dispensable. After incubation the Anti-GFP-HRP antibody can be directly detected using commercially available chemiluminescent reagents. The monoclonal Anti-GFP antibody specifically reconizes wild-type GFP from Aequorea victoria and derivatives, e.g., EGFP, CFP, YFP, and BFP. - 中国
Goat Polyclonal GFP antibody for FLISA, ICC, FACS, IHC (fro), IF, PLA, WB. Published in 7 Pubmed References. Order this anti-GFP antibody. | Product number ABIN151299
Anti-GFP antibody conjugated to Biotin [LGB-1] validated for WB. Referenced in 1 publication. Immunogen corresponding to recombinant full length protein
Lack of expression of dystrophin leads to degeneration of muscle fibers and infiltration of connective and adipose tissue. Cell transplantation therapy has been proposed as a treatment for intractable muscle degenerative disorders. Several reports have demonstrated the ability of bone-marrow derived cells (BMDC) to contribute to non-haematopoietic tissues including epithelium, heart, liver, skeletal muscle and brain following transplantation by means of fusion and reprogramming. A key issue is the extent to which fusion and reprogramming can occur in vivo, particularly under conditions of myogenic deterioration.To investigate the therapeutic potential of bone marrow transplantation in monogenetic myopathy, green fluorescent protein-positive (GFP+) bone marrow cells were transplanted into non-irradiated c-kit receptor-deficient (W⁴¹) mdx mice. This model allows BMDC reconstitution in the absence of irradiation induced myeloablation. We provide the first report of BMDC fusion in a ...
The recruitment of monocytes and their differentiation into macrophages at sites of inflammation are key events in determining the outcome of the inflammatory response and initiating the return to tissue homeostasis. To study monocyte trafficking and macrophage differentiation in vivo, we have generated a novel transgenic reporter mouse expressing a green fluorescent protein (GFP) under the control of the human CD68 promoter. CD68-GFP mice express high levels of GFP in both monocyte and embryo-derived tissue resident macrophages in adult animals. The human CD68 promoter drives GFP expression in all CD115(+) monocytes of adult blood, spleen, and bone marrow; we took advantage of this to directly compare the trafficking of bone marrow-derived CD68-GFP monocytes to that of CX3CR1(GFP) monocytes in vivo using a sterile zymosan peritonitis model. Unlike CX3CR1(GFP) monocytes, which downregulate GFP expression on differentiation into macrophages in this model, CD68-GFP monocytes retain high-level GFP
TY - JOUR. T1 - Studying cytoskeletal dynamics in living cells using green fluorescent protein. AU - Yoon, Yisang. AU - Pitts, Kelly. AU - McNiven, Mark. PY - 2002/7/4. Y1 - 2002/7/4. N2 - Microfilaments, intermediate filaments, and microtubules are three major cytoskeletal systems providing cells with stability to maintain proper shape. Although the word "cytoskeleton" implicates rigidity, it is quite dynamic exhibiting constant changes within cells. In addition to providing cell stability, it participates in a variety of essential and dynamic cellular processes including cell migration, cell division, intracellular transport, vesicular trafficking, and organelle morphogenesis. During the past eight years since the green fluorescent protein (GFP) was first used as a marker for the exogenous gene expression, it has been an especially booming era for live cell observations of intracellular movement of many proteins. Because of the dynamic behavior of the cytoskeleton in the cell, GFP has ...
References for The Development of New FPs 15. Shaner , N C, Patterson, G H, & Davidson, M W. (2007). Advances in fluorescent protein technology . Journal of Cell Science, 120. PMID: 18057027 16. Shaner, N C, Steinbach, P A & Tsien, R Y. (2005). A guide to choosing fluorescent proteins. Nature Methods, 2, PMID: 16299475 17. Ai, H, Shaner, N C, Cheng, Z, Tsien, R Y & Campbell, R E. (2007). Exploration of new chromophore structures leads to the identification of improved blue fluorescent proteins. Biochemistry, 46, PMID: 17444659 18. Kremers, G J, Goedhart, J, van den Heuvel, D J, Gerritsen, H C. & Gadella, T W J. (2007). Improved green and blue fluorescent proteins for expression in bacteria and mammalian cells. Biochemistry, 46, PMID: 17323929 19. Cubitt, A B., Heim, R, Adams, S R, Boyd, A E, Gross, L A & Tsien, R Y. (1995). Understanding, improving and using green fluorescent proteins. Trends Biochemical Science, 20, PMID: 8578587 20. Rizzo, M A, Springer, G H, Granada, B & Piston, D W. (2004). ...
References for The Development of New FPs 15. Shaner , N C, Patterson, G H, & Davidson, M W. (2007). Advances in fluorescent protein technology . Journal of Cell Science, 120. PMID: 18057027 16. Shaner, N C, Steinbach, P A & Tsien, R Y. (2005). A guide to choosing fluorescent proteins. Nature Methods, 2, PMID: 16299475 17. Ai, H, Shaner, N C, Cheng, Z, Tsien, R Y & Campbell, R E. (2007). Exploration of new chromophore structures leads to the identification of improved blue fluorescent proteins. Biochemistry, 46, PMID: 17444659 18. Kremers, G J, Goedhart, J, van den Heuvel, D J, Gerritsen, H C. & Gadella, T W J. (2007). Improved green and blue fluorescent proteins for expression in bacteria and mammalian cells. Biochemistry, 46, PMID: 17323929 19. Cubitt, A B., Heim, R, Adams, S R, Boyd, A E, Gross, L A & Tsien, R Y. (1995). Understanding, improving and using green fluorescent proteins. Trends Biochemical Science, 20, PMID: 8578587 20. Rizzo, M A, Springer, G H, Granada, B & Piston, D W. (2004). ...
PubMed comprises more than 30 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
The assessment of how many cells have been successfully transfected or transduced in a cell population is a basic and critical evaluation parameter in many cell and molecular biology labs. Commonly, the cells of interest are transfected or transduced with a construct that results in the expression of a fluorescent protein (FP) reporter, such as GFP.
Clontech offers two improved green fluorescent proteins: AcGFP1 is a monomeric green fluorescent protein and ZsGreen1 is extremely bright.
Clontech offers two improved green fluorescent proteins: AcGFP1 is a monomeric green fluorescent protein and ZsGreen1 is extremely bright.
Sino Biologcial offers high quality green fluorescent protein GFPSpark® with features of bright green fluorescence, high pH-stability and fast maturation.
The recent emergence of an autofluorescent protein, the green fluorescent protein (GFP), has opened the door for the convenient use of intact living cells and organisms as experimental systems in fields ranging from cell biology to biomedicine. We present an overview of some of the major applications of GFP, namely its use in protein tagging and in monitoring gene expression as well as its potential in a variety of biological screens.. ...
Since the discovery of the green fluorescent protein gene, fluorescent proteins have impacted molecular cell biology. This protocol...
Anti-GFP antibody (ab6556) has been cited in 498 publications. References for Human, Mouse, Rat in ChIP, EM, Flow Cyt, ICC, ICC/IF, IF, IHC, IHC (PFA fixed)…
TY - JOUR. T1 - Antiparallel leucine zipper-directed protein reassembly. T2 - Application to the green fluorescent protein [12]. AU - Ghosh, Indraneel. AU - Hamilton, Andrew D.. AU - Regan, Lynne. PY - 2000/6/14. Y1 - 2000/6/14. UR - http://www.scopus.com/inward/record.url?scp=0034647238&partnerID=8YFLogxK. UR - http://www.scopus.com/inward/citedby.url?scp=0034647238&partnerID=8YFLogxK. U2 - 10.1021/ja994421w. DO - 10.1021/ja994421w. M3 - Letter. AN - SCOPUS:0034647238. VL - 122. SP - 5658. EP - 5659. JO - Journal of the American Chemical Society. JF - Journal of the American Chemical Society. SN - 0002-7863. IS - 23. ER - ...
Page contains details about PrS/enhanced green fluorescent protein plasmid bilayers . It has composition images, properties, Characterization methods, synthesis, applications and reference articles : nano.nature.com
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Figure 3. Characterization of NEX-CRE mice and analysis of Itgb1 expression by flow cytometry. a-n , Z/EG reporter mice carrying a CRE-inducible GFP transgene were crossed with NEX-CRE mice to analyze the CRE recombination pattern. a-c , GFP fluorescence was evident in the developing cerebral cortex of E12.5-E16.5 embryos by whole-mount analysis. d , GFP fluorescence throughout the cerebral cortex was also evident in vibratome sections. e-h , Coronal sections of mice at E14.5 and E16.5 were stained with antibodies to GFP. GFP expression was evident in the SVZ and cortical plate (CP), but not in the VZ. In e and g , nuclei were counterstained with DAPI (blue). i-k , Higher-magnification views of coronal sections stained with DAPI and antibodies to GFP. The vast majority of cells were GFP positive (arrows). l-n , Sections from E15.5 animals were stained with antibodies to doublecortin (dcx, red) and GFP (green). ( l′-n′ ) Higher-magnification views of the area outlined in l-n . Note ...
In addition to XLacZ+/- mosaics, the suitability of transgenic mice from lines Y001deltaDRR and Y223 [30] (both displaying mosaic corneal GFP expression) were evaluated for wound healing studies. These animals carry a yeast artificial chromosome (YAC) containing the human PAX6 locus [31] into which a GFP reporter gene has been inserted at the PAX6 ATG start codon, placing it under the control of the PAX6 regulatory elements. This also eliminates production of PAX6 protein from the YAC ensuring that wild type Pax6 levels in these mice are unaffected. Both lines demonstrated patterns of GFP-positive and GFP-negative radial corneal stripes (Fig. 5) qualitatively similar to those observed in XLacZ+/- mosaics (Fig. 2). The reason for the mosaic transgene expression is not clear but it probably involves stochastic transgene inactivation early in development so that only a proportion of adult limbal stem cells express GFP. Line Y001 contains a single copy of the YAC with a 10-20 kb truncation, while ...
A cDNA, ERD1, isolated from one-hour-dehydrated plants of Arabidopsis thaliana L. encodes a putative protein that is similar to the regulatory ATPase subunit (ClpA) of the Clp protease and contains a putative chloroplast-targeting transit-peptide at the N-terminus. A chimeric gene with the putative plastid-targeting sequence of the erd1 gene fused to the synthetic green-fluorescent protein (sGFP ...
Unlike enzyme markers, green fluorescent protein can be visualized at high resolution in living cells using confocal microscopy. The images are not prone to fixation or staining artifacts, and can be of exceptional clarity. Moreover, the activities of living cells, such as cytoplasmic streaming, are …
A quantitative investigations of plants expressing green fluorescent protein (GFP) using a home-made camera attachment In this laboratory exercise students use digital cameras to measure surface fluorescence of transgenic plants expressing green fluorescence protein (GFP). Inquiry pedagogy is used to teach processes used in scientific investigations and how GFP is used in reporter gene systems. Students start by making observations involving induction of expression of a GFP expressing reporter gene and then.... Publisher: EvoEd Digital ...
After 24 hrs,EGFP-expressing cells have been picked in Asarylaldehyde the presence of 1 mg/mL G418 and colonies have been expanded.EGFP virus was harvested in the PT67 cells and put to use to infect 231-BR cells.The following day,231-BR cells had been selected within the presence of 0.8 mg/mL G418.EGFPexpressing cells had been then co-transfected with pCMV4.HER2 fulllength human cDNA and pSVzeo to confer antibiotic resistance.The sequence in the HER2 insert in pCMV4.HER2 was confi rmed by sequencing.Secure colonies had been picked during the presence of 0.750 mg/mL zeocin.A vector management cell line was simultaneously established by transfecting both pCMV4 that lacked inserted cDNA and pSVzeo in to the 231-BR-EGFP cells and deciding on secure colonies during the presence of 0.750 mg/mL zeocin.The 231-BR cells that had been transfected with vectors that contained or lacked the HER2 cDNA were maintained in Dulbeccos modifi ed Eagle Medium supplemented with 10% fetal bovine serum and 1% ...
All greens need a thorough washing in lukewarm water to remove soil. Fill a large bowl with lukewarm water and move the greens up and down in the water several times. Then lift the greens out of water so that any sand or soil will remain on the bottom of the bowl. Some greens may require two washings. After washing, pat greens with a clean towel or use a salad spinner to dry them. Leave young greens whole, but cut the stems off of larger, older greens.. Mild greens, such as spinach, kale, and chard can be steamed, boiled, or eaten raw. If you choose to cook them, such greens should be cooked quickly to preserve their bright green color. Stronger-flavored greens, such as collard, mustard, and turnip greens, should be blanched before cooking or adding to soups or stews, to remove objectionable odors and bitter flavors. (Refer to blanching instructions below.) Remember that greens will cook down to between a quarter and a half of their original volume, so buy accordingly.. Raw: Raw greens, with ...
mTurquoise is a basic (constitutively fluorescent) cyan fluorescent protein published in 2010, derived from Aequorea victoria. It has low acid sensitivity.
From Jellyfish to the Bench: How Green Fluorescent Protein Is Used in Research Students may be familiar with green fluorescent protein (GFP) from classwork or seeing transgenic animals in their local pet store. However, they may not know that its also an important research tool. Use this overview to help them discover how scientists use GFP to study many of the major questions in biology. View » ...
From Jellyfish to the Bench: How Green Fluorescent Protein Is Used in Research Students may be familiar with green fluorescent protein (GFP) from classwork or seeing transgenic animals in their local pet store. However, they may not know that its also an important research tool. Use this overview to help them discover how scientists use GFP to study many of the major questions in biology. View » ...
These NG-BAC transgenic mice express enhanced green fluorescent protein (EGFP) directed by the |i|Rag2|/i| promoter, and may be used in studies of B and T cell development as EGFP fluorescence reflects |i|Rag2|/i| protein expression.
Now i have a new problem! This BrdU protocol needs Denaturation. Now i want to monitor GFP-transfected cells too. The HCl seems to denature the endogeneous GFP too. Is there a more acid resistable GFP? Perhaps an anti-GFP antibody? Or may i choose another method to denaturize the DNA for the anti-BrdU? Maybe temp.?Or stain for myc-tagged GFP ...
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Gamillus is a GFP cloned from Olindias formosa (flower hat jellyfish) and exhibits superior acid tolerance (pKa=3.4) and nearly twice as much brightness compared with the reported GFPs. The fluorescence spectrum is constant between pH4.5 and 9.0, which falls between the intracellular range in most cell types. X-ray crystallography (a technique used for determining the atomic and molecular structure of a crystal, in this case, a Gamillus crystal) and point mutagenesis suggest the acid tolerance of Gamillus is attributed to stabilization of deprotonation in its chemical structure. The findings were published in Cell Chemical Biology ...
What makes a good photoconvertable GFP for single particle tracking (SPT)? Well, all the things that make a good label (bright, stable, fast to mature, small, inert, this convenient and nicely separated adsorbance and emission spectra - the obvious stuff) and one more thing that is not so obvious ...
High throughput (HTS) and high content screening (HCS) assays often utilize cell sources, which are not amenable to large-scale screening and can require extensive cell culture and processing prior to imaging. To overcome this hurdle, Hera and a commercial partner ArunA Biomedical, engineered human neural progenitors derived from stem cells with a non-viral vector encoding a GFP reporter gene using a selectable piggyBac™ gene editing system to produce hNP1 GFP+ cells. hNP1 reporter cells exhibit detectable dose dependent response to toxins tested, (cytotoxicity and cell migration) providing a sensitive, high throughput and high content amenable, cell based human neurotox assay platform.. ...
The health quotient of this fish steamed on greens recipe is a bit off the charts. Lean protein from fish, fiber and vitamins and minerals from the greens.
Anti-GFP, anti-RFP, and anti-mCherry antibodies provide a convenient method for visualizing GFP, RFP, or mCherry proteins respectively, especially when amplification of the fluorescent protein of interest is necessary to overcome a weak or degraded signal.. Our anti-fluorescent protein antibodies are highly specific, reacting only with the intended target and thereby contributing to low background signal and an increased signal to noise ratio. They are easily incorporated into standard immunostaining protocols for cell and tissue analysis,enabling consistent and reliable detection of fluorescent proteins and fluorescent protein fusions in western blot and flow cytometry applications. ...
Transfection of the different EGFP fusion constructs to IMR-90 fibroblasts. A) The wild-type SAP30L concentrates in small dense bodies in the nuclei. B) The nuc
The original immunofluorescence technique introduced by AH COONS and co-workers about 60 years ago has been refined during the last two decades in a manifold way. New developments such as confocal, deconvolution, ratio-imaging, total internal reflection and applications of fluorescent reporter molecules (such as green fluorescent protein [GFP] and variants, briefly called FP for fluorescent proteins) have now become integral tools in functional genomics and cell development. In functional genomics, transgenic approches using FPs might well be exploited to examine promoter activity and to clone regulatory elements ...
3ème journée de réflexion de lEcole Doctorale Thématique en Génie des Procédés: Lintensification des procédés du point de vue académique et industriel ...
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Plasmid pSpCas9n(BB)-2A-GFP (PX461) from Dr. Feng Zhangs lab contains the insert hSpCas9n and is published in Nat Protoc. 2013 Nov;8(11):2281-308. doi: 10.1038/nprot.2013.143. Epub 2013 Oct 24. This plasmid is available through Addgene.
miniSOG Q103V is a basic (constitutively fluorescent) cyan fluorescent protein published in 2016, derived from Arabidopsis thaliana. It requires the cofactor flavin for fluorescence.
The discovery reveals the role of a growth factor and endothelial cells in thymus repair, and could have implications for chemotherapy and radiation patients recovery following treatment.. 0 Comments. ...
In chapter 3, "The Sense of Sensibility," author Wendy Jones uses scenes from one of Jane Austens most celebrated novels to illustrate the functioning of the bodys stress response system.. 0 Comments. ...
Protein Kinase Of Unknown Cellular Role; Green Fluorescent Protein (GFP)-fusion Protein Localizes To The Cytoplasm And Nucleus; Null Mutant Is Sensitive To Expression Of The Top1-T722A Allele; Not An Essential Gene; Relocalizes From Nucleus To Cytoplasm Upon DNA Replication Stress