Increasing β-cell mass is one of the mechanisms by which glucose homeostasis is maintained in vivo in response to physiological (i.e., growth and gestation) and pathophysiological (i.e., obesity and insulin resistance) stimuli. Unlike other terminally differentiated cell types, pancreatic β-cells retain their ability to proliferate under both physiological and pathophysiological conditions (3). In the progression of diabetes, insulin secretion is increased, and a large amount of the increase in the first stage of this compensation process is due to increased β-cell mass, which is mainly achieved through an increase in β-cell number (24).. The gene encoding for Grb10 is imprinted in mammalian cells, and its expression is defined by its parental origins (25). The maternal allele determines Grb10 expression in all tissues except the brain, and the paternal allele determines Grb10 expression in the brain. Grb10 is expressed in insulin target tissues such as fat and muscle in vivo. The unique ...

Purified Recombinant Mouse Grb7 Protein, His-tagged from Creative Biomart. Recombinant Mouse Grb7 Protein, His-tagged can be used for research.

Defects in insulin secretion and reduction in β-cell mass are associated with type 2 diabetes in humans and understanding the basis for these dysfunctions may reveal strategies for diabetes therapy. In this study, we show that pancreas-specific knockout of growth factor receptor-binding protein 10 (Grb10), which is highly expressed in pancreas and islets, leads to elevated insulin/IGF-1 signaling in islets, enhanced β-cell mass and insulin content, and increased insulin secretion in mice. Pancreas-specific disruption of Grb10 expression also improved glucose tolerance in mice fed with a high-fat diet and protected mice from streptozotocin-induced β-cell apoptosis and body weight loss. Our study has identified Grb10 as an important regulator of β-cell proliferation and demonstrated that reducing the expression level of Grb10 could provide a novel means to increase β-cell mass and reduce β-cell apoptosis. This is critical for effective therapeutic treatment of both type 1 and 2 diabetes. ...

Original model: BIOMD0000000048 The epidermal growth factor receptor (EGFR) is part of the protein-tyrosine kinase receptor family. It regulates cell growth, survival, proliferation and differentiation. Epidermal growth factor binds to the EGFR, and facilitates receptor dimerization and rapid activation of its intrinsic tyrosine kinase, followed by autophosphorylation of multiple tyrosine residues in the cytoplasmic receptor domain. This tyrosine phosphorylation of EGFR triggers a number of biochemical reactions involving various protein domains (as an example, see Figure 1 for the targets involved in tumor cells [1]). Kholodenko et al developed the first detailed kinetic model of EGF receptor function [2] (a similar model was created in parallel by Bhalla and Iyengar [3]). The authors focus on the activation of the growth factor receptor-binding protein 2 (Grb2), Src homology and collagen domain protein (Shc) and phosholipase C-γ (PLC-γ). Pathways involving Grb2 and Shc lead to the activation ...

Buy our Recombinant Human GRB2 protein. Ab116430 is a full length protein produced in Escherichia coli and has been validated in WB, ELISA, SDS-PAGE, MS. Abcam…

The RT² qPCR Primer Assay is the most reliable SYBR® Green-based quantitative real-time PCR assay for gene expression analysis. Our experimentally verified design algorithm yields gene-specific qPCR assays characterized by uniform and high PCR efficiencies and standardized amplification conditions. Every RT² Primer Assay is subjected to rigorous experimental verification. Single product amplification of the correct size and high PCR efficiency are guaranteed when using the appropriate RT² qPCR Master Mixes. The uniform PCR amplification efficiencies and PCR conditions of the RT² qPCR Primer Assays provide an accurate and scalable solution for multiple gene expression analyses. Browse Primer Assays By Gene ...

HPA005788: Rabbit Polyclonal Anti-GRAP2 Antibody against Human GRB2-related adaptor protein 2. Validated for IF, IHC, WB. Order at Bio-Connect.

Complete information for GRB2 gene (Protein Coding), Growth Factor Receptor Bound Protein 2, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium

Complete information for GRB2 gene (Protein Coding), Growth Factor Receptor Bound Protein 2, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium

TITLE: GCN GRB OBSERVATION REPORT NUMBER: 2156 SUBJECT: GRB030329: 444 AAVSO Measurements DATE: 03/04/20 03:21:34 GMT FROM: AAVSO GRB Network at AAVSO ...

TY - JOUR. T1 - Immunity to growth factor receptor-bound protein 10, a signal transduction molecule, inhibits the growth of breast cancer in mice. AU - O-Sullivan, In Sug. AU - Chopra, Amla. AU - Carr, Janai. AU - Tae, Sung Kim. AU - Cohen, Edward P.. PY - 2008/4/1. Y1 - 2008/4/1. N2 - This study describes the application of a unique strategy to identify breast cancer antigens [tumor-associated antigen (TAA)]. In a mouse model, the strategy led to the identification of growth factor receptor-bound protein 10 (Grb10) as a newly identified TAA. Grb10 is a signal transduction molecule associated with multiple transmembrane tyrosine kinase receptors. It was discovered by comparing microarrays of cellular breast cancer vaccines highly enriched for cells that induced breast cancer immunity in tumor-bearing mice with nonenriched vaccines. The vaccines were prepared by transferring a cDNA expression library derived from SB5b cells, a breast cancer cell line C3H/He origin (H-2k), into LM mouse ...

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GRB2 (Growth factor receptor-bound protein 2), Authors: Gagani Athauda, Donald P Bottaro. Published in: Atlas Genet Cytogenet Oncol Haematol.

TY - JOUR. T1 - The human GRB2 and Drosophila Drk genes can functionally replace the Caenorhabditis elegans cell signaling gene sem-5. AU - Stern, M. J.. AU - Marengere, L. E.M.. AU - Daly, R. J.. AU - Lowenstein, E. J.. AU - Kokel, M.. AU - Batzer, A.. AU - Olivier, P.. AU - Pawson, T.. AU - Schlessinger, J.. PY - 1993/1/1. Y1 - 1993/1/1. N2 - Mutations in the Caenorhabditis elegans gene sem-5 affect cell signaling processes involved in guiding a class of cell migrations and inducing vulval cell fates. The sem-5 sequence encodes a protein comprised almost exclusively of SH2 and SH3 domains (SH, src homology region) that are found together in many signaling proteins and nonreceptor tyrosine kinases. A human protein, GRB2, was identified by its ability to associate with the activated human epidermal growth factor receptor (hEGFR). The GRB2 and Sem-5 proteins share an identical architecture of their SH2 and SH3 domains and 58% amino acid sequence identity. Here we demonstrate that GRB2 and a ...

InterPro provides functional analysis of proteins by classifying them into families and predicting domains and important sites. We combine protein signatures from a number of member databases into a single searchable resource, capitalising on their individual strengths to produce a powerful integrated database and diagnostic tool.

1IO6: Solution Structure of the C-Terminal SH3 Domain of Grb2 Complexed with a Ligand Peptide: A Ligand Exchange Model of the SH3 Domain

Granzyme B (GrB) è una proteasi di tipo serinico espressa dai linfociti citotossici (linfociti T e cellule NK), che in presenza di perforina (Pf), induce apoptosi nelle cellule bersaglio quali cellule infettate e cellule tumorali. Recentemente, è stato dimostrato che GrB è espressa, spesso in assenza di Pf, anche in cellule non-linfoidi; tuttavia il ruolo di GrB in queste cellule non è stato ancora definito. Lobbiettivo della mia ricerca è stato quello di indagare: - lespressione di GrB e Pf in linee cellulari e in tessuti di carcinoma della vescica, mediante RT-PCR, Western Blot, ELISA, immunofluorescenza e immunoistochimica. - la funzione di GrB in vitro mediante saggi di attività enzimatica e saggi di invasione cellulare, in presenza o in assenza di silenziamento genico di GrB. I risultati di questo studio hanno rivelato lespressione di GrB, in assenza di Pf, in cellule e tessuti di carcinoma uroteliale (UC). Differenze significative sono state riscontrate tra lespressione di GrB e ...

These |i|Grb2|/i| floxed mice may be useful for studying the effect of GRB2-dependant signal transduction on lymphocyte differentiation and immune response.

Check out A study of the prompt and afterglow emission of the short GRB 061201. Rate and share your experience with other people.

1 MEAIAKYDFK ATADDELSFK RGDILKVLNE ECDQNWYKAE LNGKDGFIPK NYIEMKPHPW 61 FFGKIPRAKA EEMLSKQRHD GAFLIRESES APGDFSLSVK FGNDVQHFKV LRDGAGKYFL 121 WVVKFNSLNE LVDYHRSTSV SRNQQIFLRD IEQVPQQPTY VQALFDFDPQ EDGELGFRRG 181 DFIHVMDNSD PNWWKGACHG QTGMFPRNYV TPVNRNV ...

ヤギ・ポリクローナル抗体 ab2234 交差種: Ms,Rat,Hu,Pig 適用: WB,ELISA,IHC-P…GRB2抗体一覧…画像、プロトコール、文献などWeb上の情報が満載のアブカムの Antibody 製品。国内在庫と品質保証制度も充実。

Looking for online definition of growth factor receptor-bound protein 7 in the Medical Dictionary? growth factor receptor-bound protein 7 explanation free. What is growth factor receptor-bound protein 7? Meaning of growth factor receptor-bound protein 7 medical term. What does growth factor receptor-bound protein 7 mean?

Aberrant activation of receptor tyrosine kinases, such as epidermal growth factor receptor (EGFR), plays a role in cancer initiation, progression, and acquired drug resistance. Genetic analysis may not always reveal aberrant activity of receptor tyrosine kinase signaling; thus, detecting active signaling through these kinases in clinical samples should improve prognostication and personalization of therapies. Diverse cancers, for example, those found in the lung, colon, or head and neck, can have aberrant activation of EGFR signaling, and EGFR-targeted therapies are used to treat these diseases. Smith et al. developed a proximity ligation assay (PLA) to detect the interaction between EGFR and the requisite signaling adaptor GRB2 (growth factor receptor-bound protein 2) in common clinical preparations. EGFR:GRB2 PLA recapitulated traditional readouts of active EGFR signaling in cultured cells, in a panel of tumor xenografts in mice derived from primary patient samples, and in samples from three ...

2506 The tumor suppressing ability of p27Kip1 (Kinase Inhibitory Protein 1) is commonly assigned to its cyclin dependent kinase (CDK) inhibitory function. However, we recently identified a separable and distinct role for p27 regulating Growth Factor Receptor-Bound Protein 2 (GRB2). GRB2 participates in key signaling pathways often hyperactivated in human cancers. Conversely, p27 function is often disrupted in human cancers, and loss of p27 function may contribute to tumorigenesis by compromising GRB2-mediated signal transduction. We now show p27 regulates GRB2 in untransformed breast epithelial cells. This pathway is temporally disrupted in breast cancer cells, which contain excess cyclin D1-CDK4 which sequesters p27 destined to target GRB2. Our results describe the first example of transformed cells compromising a p27 function other than CDK inhibition, and thus provide an alternative explanation of why p27 is frequently disrupted in human cancers. ...

Binding of growth factor ligands to extracellular domain, RTKs induces receptor dimerization (forming either homodimers or heterodimers with other RTKs) followed by their autophosphorylation. This allows for subsequent recruitment of adaptor proteins such as growth factor receptor-bound protein 2 (GRB2), SRC homology 2 domain-containing protein (SHC), and Son of Sevenless (SOS) that activate proliferation, survival, and migration pathways (e.g., RAS, PI3K, SRC ...

Chronic myeloid leukemia is characterized by the Philadelphia (Ph1) translocation t(9;22) that generates a hybrid gene, bcr/abl, translated to a Mr210,000 tyrosine kinase (p210bcr/abl) with transforming activity for hematopoietic cells. Hematopoietic cell transformation by p210bcr/abl seems to involve activation of the Ras signaling pathway by at least two different signaling intermediates, growth factor receptor-bound protein 2 and Src homology and collagen protein, but additional signaling proteins are likely to be required as well. In an effort to identify additional phosphoproteins activated by p210bcr/abl, we studied the murine, interleukin 3-dependent, myeloid cell line, 32D, and a bcr/abl-transfected, factor-independent subline, 32Dp210. The analysis of whole-cell lysates of 32D and 32Dp210 cells showed that several proteins with a molecular weight of Mr50,000-60,000 were phosphorylated on tyrosine residues in 32Dp210 cells. Because Src family kinases have an apparent molecular weight of ...

To identify receptor-associated proteins that may contribute to the specificity of insulin and IGF-I signaling responses, a mouse embryo library was screened using the yeast two-hybrid system. Multiple receptor-interactive clones encoding the SH2 domain of the adapter protein Grb10 were isolated. Subsequent cloning of the full-length Grb10 sequence from a mouse fat cDNA library defined a previously unknown Grb10 variant, that appears to be the predominant isoform in mouse tissues. Receptor-deficient R- cells (fibroblasts from mice with homologous disruption of the IGF-I receptor gene) and transfected R- cells expressing either insulin receptors (R-IR cells) or IGF-I receptors (R+ cells) were used to investigate the specificity of Grb10 interaction with the two related receptors. Hormone-activated insulin receptors in R-IR cells coprecipitated with three species, all recognized as Grb10 isoforms by specific Grb10 antibody. Under the same conditions, Grb10 was essentially undetectable in IGF-I ...

GRB2 antibody detects GRB2 protein by Western blot analysis.A. 30 μg THP-1 whole cell lysate/extract12 % SDS-PAGEGRB2 antibody (GTX101176) dilution: 1: ...

Recombinant GRB2-Related Adaptor Protein (GRAP) Protein (Myc-DYKDDDDK Tag). Species: Human. Source: HEK-293 Cells. Order product ABIN2722222.

SGIP1 (SH3 domain GRB2 like endophilin interacting protein 1), Authors: Dessen P. Published in: Atlas Genet Cytogenet Oncol Haematol.

We present motivations for and study feasibility of a small, rapid-optical/IR response gamma-ray burst (GRB) space observatory. By analyzing existing GRB data, we give realistic detection rates for X-ray and optical/IR instruments of modest size under actual flight conditions. Given new capabilities of fast optical/IR response (~1 s to target) and simultaneous multi-band imaging, such an observatory can have a reasonable event rate, likely leading to new science. Requiring a Swift-like orbit, duty cycle, and observing constraints, a Swift-BAT scaled down to 190 cm2 of detector area would still detect and locate about 25 GRB yr-1 for a trigger threshold of 6.5σ. About 23% of X-ray located GRB would be detected optically for a 10 cm diameter instrument (~6 yr-1 for the 6.5σ X-ray trigger). ...

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TITLE: GCN CIRCULAR NUMBER: 14003 SUBJECT: GRB 121123A: Swift/UVOT Observations DATE: 12/11/26 22:25:09 GMT FROM: Stephen Holland at STScI ...

Hop on to get the meaning of Gab2 acronym / slang / Abbreviation. The Medical & Science Acronym / Slang Gab2 means... AcronymsAndSlang. The Gab2 acronym/abbreviation definition. The Gab2 meaning is Grb2-associated binder 2. The definition of Gab2 by AcronymAndSlang.com

When assisting a patient from bed to wheelchair, you should:1. elevate head of bed.2. lower the bed as far as possible.3. use the face-to-face assist to help them stand.4. assist the patient to pivot with back to wheelchair before sitting down ...

GRB011212: Optical Observations A. Dullighan, G. Monnelly, G. Ricker, R. Vanderspek, N. Morgan (MIT); and D. Tholen (U.Hawaii) write: We have observed the error box of the HETE GRB011212 (GCN #1194) with the Baade 6.5m telescope at Magellan on 2001 December 14.17 UT (Epoch 1) and December 15.17 UT (Epoch 2) using the MagIC CCD detector. Twelve 360s R-band exposures were used to mosaic the entire HETE and RXTE/ASM joint error box on each night. Both nights had a seeing of 0.7 with a limiting magnitude of 24.5, except for the last half of the second night when the seeing decreased slowly to 1.3, with a limiting magnitude of 24.0 on the last frame. Third epoch observations were made with the University of Hawaii 2.2m telescope on Mauna Kea on 2002 February 16.33 and 17.38 UT. Three frames were used to mosaic the error box each with three 600s VR-band exposures under conditions of 1.3 seeing, giving a limiting magnitude of 24.5 after summing. Aperture photometry was performed on all objects ...

Scientists may soon help people grow strong muscles without needing to hit the weight room. As reported in The FASEB Journal, researchers discovered that by blocking the function of the protein Grb10 in mice in the womb, they developed as more muscular than their normal counterparts. This presents important implications for a range of conditions that are worsened by, or cause muscle wasting, such as injury, muscular dystrophy, and Type 2 diabetes.

Abstract: Very recently, the gravitational-wave (GW) event GW170817 was discovered to be associated with the short gamma-ray burst (GRB) 170817A. Multi-wavelength follow-up observations were carried out, and X-ray, optical, and radio counterparts to GW170817 were detected. The observations undoubtedly indicate that GRB 170817A originates from a binary neutron star merger. However, the GRB falls into the low-luminosity class that could have a higher statistical occurrence rate and detection probability than the normal (high-luminosity) class. This implies the possibility that GRB 170817A is intrinsically powerful, but we are off-axis and only observe its side emission. In this Letter, we provide a timely modeling of the multi-wavelength afterglow emission from this GRB and the associated kilonova signal from the merger ejecta, under the assumption of a structured jet, a two-component jet, and an intrinsically less-energetic quasi-isotropic fireball, respectively. Comparing the afterglow ...

May play a role in subcellular trafficking between the endoplasmic reticulum and Golgi apparatus through interactions with the Rho-type GTPases. Binding to the NS3 protein of dengue virus type 2 appears to subvert this activity into the alteration of the intracellular membrane structure associated with flaviviral replication.

14 January 2015: Imprinted genes are monoallelic expressed in a parent-of-origin dependent manner (Jirtle and Weidman 2007).The conflict theory of genomic imprinting predicts that maternally expressed genes are antigrowth while paternally expressed genes are progrowth (Haig and Graham 1991). The first two genes experimentally identified to be imprinted, the maternally expressed Igf2r (Barlow et al. 1991) and the paternally expressed Igf2 (DeChiara et al. 1991), were shown over two decades ago to adhere to this prediction. A second set of oppositely imprinted, fetal growth antagonistic genes has now been identified, the maternally expressed Grb10 and the paternally expressed Dlk1 (Madon-Simon et al. 2014). Grb10 encodes for an intracellular signaling adaptor protein that restricts fetal growth and promotes fat deposition. In contrast, Dlk1 encodes for a ligand that promotes fetal growth and restricts fat deposition. Deregulation of DLK1 expression by a single point mutation also results in the ...

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We are doing the first experiment for IFN-g detecting by the new AID machine. Then we will use the IFN-g/GrB fluorospot kit to evaluate the activity of cyto...

Bio-Path Holdings, Inc. is a clinical and preclinical stage oncology focused antisense drug development company. The Company utilizes a technology that achieves systemic delivery for target specific protein inhibition for any gene product that is over-expressed in disease. Its drug delivery and antisense technology, DNAbilize, is a platform that uses P-ethoxy, a deoxyribonucleic acid backbone modification. Its lead drug candidate, Liposomal Grb2 (BP1001), targets the protein Growth factor receptor-bound protein 2 (Grb2). Its other liposome delivered antisense drug candidate, Liposomal Bcl2 (BP1002), targets the protein B-cell lymphoma 2 (Bcl2). BP1001 is in Phase II clinical trials for acute myeloid leukemia, and for blast phase and accelerated phase chronic myelogenous leukemia. BP1002 is intended to target the lymphoma and certain solid tumor markets. BP1001 is also in preclinical studies for solid tumors, including triple negative breast cancer and inflammatory breast cancer.. ...

TORICH ASTM A500 GrB 75x75 Cold Formed Carbon Pipe ...TORICH ASTM A500 GrB 75x75 Cold Formed Carbon Pipe Structural Hollow Section Mild MS Profile Square Steel Tube For Construction, US $ 1000 - 1800 / Metric Ton, ASTM ...

Breast cancer is widely and effectively treated with endocrine treatment. However, in many cases the tumours will eventually progress into

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FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a signaling adaptor protein with two EH domains that interacts with proteins that participate in signaling, endocytosis and cytoskeletal changes. The encoded protein has been found in association with intersectin 1 and Src homology 3-domain growth factor receptor-bound 2-like (endophilin) interacting protein 1 when intersectin 1 was isolated from clathrin-coated pits. The encoded protein has also been shown to interact with amphiphysin, a cytoplasmic protein at the surface of synaptic vesicles. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Mar 2014 ...

Myc-DDK-tagged ORF clone of Homo sapiens GRB10 interacting GYF protein 2 (GIGYF2), transcript variant 1 as transfection-ready DNA - 10 µg - OriGene - cdna clones

The corrosion on the flanged ring just adds insult to injury, in my opinion. Now, the auction text doesnt specifically claim that the part isnt reproduction, but it does state that its Meccano - but the only part that is Meccano is the central spider. To even the greenest collector of Meccano, these parts arent pre-war. Neither are they, in any reasonable fashion, good to good plus. At least, I dont think they are.. The only question remains on the box. Is this genuine Meccano? Was there an early (pre-1928) version of the GRB box (or indeed, a late one) like this? It remains to be seen. My hunch is that these arent genuine, simply because its in too good a condition. This GRB was clearly bought and used in a model around 1970, and bears the scars from that use. I cant imagine that this box is more than twice the age of its contents -- otherwise why would it be connected with this part? And another identical box has appeared with a reproduction part in it? But I could be wrong... the ...

GRB 777 Mini Mobile Rock Crusher 580 Watt - Gold Rat . Rock Crusher for sale Freight quotes are by request purchase price does not include freight**** All 777Minis in stock have the upgraded impact bars* This little portable rock crusher is the smallest in the GRB range.

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