A synthetic nonapeptide, which is C-terminal sequence of 94-amino acid of prostatic inhibin peptide was tested for progesterone and estrogen secretion by mouse granulosa cell cultures. Nonapeptide suppressed the progesterone and estrogen synthesis, the magnitude of suppression was highest at 5 ng dose level for progesterone and 50 ng dose level for estradiol. The study suggests that, nonapeptide exerts its effect by impairing the binding of FSH to granulosa cell receptors. ...
Poster (2015, June 14). Study question: In a model reproducing early ischemia after ovarian tissue transplantation, does the pan-caspase inhibitor Z-VAD-FMK could prevent granulosa cell apoptosis? Summary answer: Results ... [more ▼]. Study question: In a model reproducing early ischemia after ovarian tissue transplantation, does the pan-caspase inhibitor Z-VAD-FMK could prevent granulosa cell apoptosis? Summary answer: Results obtained with HGL5 granulosa cell line suggest that Z-VAD-FMK is efficient to protect granulosa cells from etoposide or CoCl2 induced apoptosis. What is known already: Removal, cryopreservation and subsequent graft of ovarian strips after cancer treatment have been successfully used to re-establish female fertility. However, the pregnancy rate after autografting of cryopreserved tissue is about 30%. Indeed, the major problem after transplantation is follicular loss due to ischemic reperfusion injury. Study design, size, duration: Three human granulosa cell lines (GC1a, ...
TY - JOUR. T1 - Arachidonic acid inhibits luteinizing hormone-stimulated progesterone production in hen granulosa cells. AU - Johnson, A. L.. AU - Tilly, J. L.. PY - 1990/1/1. Y1 - 1990/1/1. N2 - Arachidonic acid has been proposed to function as a hormone-induced second messenger in a variety of mammalian endocrine tissues. The present studies were conducted to evaluate whether arachidonic acid, either added exogenously or released endogenously following treatment with physiologic (phospholipase A2) or pharmacologic (melittin) agents, influences basal and/or luteinizing hormone (LH)-induced cyclic adenosine 3,5-monophosphate (cAMP) and progesterone production in granulosa cells from domestic hens. Phospholipase A2 (PLA2) and melittin treatments failed to alter basal concentrations of progesterone, whereas arachidonic acid had a slight stimulatory effect (only at the 50-μM dose) on progesterone levels, and no effect on cAMP. By contrast, arachidonic acid, PLA2, and melittin each inhibited ...
TY - JOUR. T1 - Dynamics of Myc/Max/Mad expression during luteinization of primate granulosa cells in vitro. T2 - Association with periovulatory proliferation. AU - Chaffin, Charles L.. AU - Brogan, Rebecca S.. AU - Stouffer, Richard L.. AU - Vandevoort, Catherine A.. PY - 2003/4/1. Y1 - 2003/4/1. N2 - Granulosa cell luteinization involves the attenuation of gonadotropin-induced proliferation. Although recent evidence indicates that primate granulosa cells stop dividing within 12 h of an ovulatory stimulus, early events in cell cycle arrest remain unknown. In the current study an in vitro model of primate granulosa cell luteinization is established that allows assessment of early events in terminal differentiation. A luteinizing dose of human chorionic gonadotropin (hCG) results in a secondary rise in proliferation before cell cycle arrest that is paralleled by a transient increase in the expression of c-Myc. In contrast, the c-Myc antagonists Mad1, Mad4, and Mxi1 are transiently repressed by ...
TY - JOUR. T1 - Differential regulation of steroidogenesis by bone morphogenetic proteins in granulosa cells. T2 - Involvement of extracellularly regulated kinase signaling and oocyte actions in follicle-stimulating hormone-induced estrogen production. AU - Miyoshi, Tomoko. AU - Otsuka, Fumio. AU - Inagaki, Kenichi. AU - Otani, Hiroyuki. AU - Takeda, Masaya. AU - Suzuki, Jiro. AU - Goto, Junko. AU - Ogura, Toshio. AU - Makino, Hirofumi. PY - 2007. Y1 - 2007. N2 - In the present study, we investigated the cellular mechanism by which oocytes and bone morphogenetic proteins (BMPs) govern FSH-induced steroidogenesis using rat primary granulosa cells. BMP-6 and BMP-7 both inhibited FSH- and forskolin (FSK)-induced progesterone synthesis and reduced cAMP synthesis independent of the presence or absence of oocytes. BMP-7 also increased FSH-induced estradiol production, and the response was further augmented in the presence of oocytes. In contrast, BMP-6 had no impact on estradiol synthesis regardless ...
TY - JOUR. T1 - Luteinizing hormone stimulates the formation of inositol trisphosphate and cyclic AMP in rat granulosa cells. Evidence for phospholipase C generated second messengers in the action of luteinizing hormone. AU - Davis, J. S.. AU - Weakland, L. L.. AU - West, L. A.. AU - Farese, R. V.. PY - 1986/1/1. Y1 - 1986/1/1. N2 - The following studies were conducted to determine whether luteinizing hormone (LH), a hormone which increases cellular levels of cyclic AMP, also provokes increases in second messengers derived from inositol lipid metabolism (i.e. inositol phosphates and diacylglycerol). Rat granulosa cells isolated from mature Graafian follicles were prelabelled for 3 h with myo-[2-3H]inositol. LH provoked rapid (5 min) and sustained (up to 60 min) increases in the levels of inositol mono-, bis-, and trisphosphates (IP, IP2 and IP3, respectively). Time course studies revealed that IP3 was formed more rapidly than IP2 and IP following LH treatment. The response to LH was ...
Mol Hum Reprod. 2014 Jan 26. [Epub ahead of print] Chang HM, Cheng JC, Taylor E, Leung PC. Author information Abstract In the ovary, connexin-coupled gap junctions in granulosa cells play crucial roles in follicular and oocyte development as well as in corpus luteum formation. Our previous work has shown that theca cell-derived bone morphogenetic protein (BMP)4 and BMP7 decrease gap junction intercellular communication (GJIC) activity via the down-regulation of connexin43 (Cx43) expression in immortalized human granulosa cells. However, the effects of oocyte-derived growth factors on Cx43 expression remain to be elucidated. The present study was designed to investigate the effects of oocyte-derived growth differentiation factor (GDF)9 and BMP15 on the expression of Cx43 in a human granulosa cell line, SVOG. We also examined the effect relative to GJIC activity and investigated the potential mechanisms of action. In SVOG cells, treatment with BMP15 but not GDF9 significantly decreased Cx43 mRNA ...
Cocaine-and amphetamine regulated transcript (CART) is an endogenous neuropeptide, which is widespread in animals, plays a key role in regulation of follicular atresia in cattle and sheep. Among animal ovaries, CART mRNA was firstly found in the cattle ovaries. CART was localized in the antral follicles oocytes, granulosa and cumulus cells by immunohistochemistry and in situ hybridization. Further research found that secretion of E2 was inhibited in granulosa cells with a certain dose of CART, the effect depends on the stage of cell differentiation, suggesting that CART could play a crucial role in regulating follicle atresia. The objective of this study was to characterize the CART expression model and hormones secretion in vivo and vitro in pig follicle granulosa cells, preliminarily studied whether CART have an effect on granulosa cells proliferation and hormones secretion in multiparous animals such as pigs. The expression levels of CART mRNA in granulosa cells of different follicles were analyzed
Cocaine-and amphetamine regulated transcript (CART) is an endogenous neuropeptide, which is widespread in animals, plays a key role in regulation of follicular atresia in cattle and sheep. Among animal ovaries, CART mRNA was firstly found in the cattle ovaries. CART was localized in the antral follicles oocytes, granulosa and cumulus cells by immunohistochemistry and in situ hybridization. Further research found that secretion of E2 was inhibited in granulosa cells with a certain dose of CART, the effect depends on the stage of cell differentiation, suggesting that CART could play a crucial role in regulating follicle atresia. The objective of this study was to characterize the CART expression model and hormones secretion in vivo and vitro in pig follicle granulosa cells, preliminarily studied whether CART have an effect on granulosa cells proliferation and hormones secretion in multiparous animals such as pigs. The expression levels of CART mRNA in granulosa cells of different follicles were analyzed
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Advanced maternal age has been shown to influence follicular and luteal dynamics in bovine ovary resulting in reduced fertility. The overall objective of the four studies presented in this thesis is to identify the maternal age-associated transcriptional changes in granulosa cells of the dominant follicles during follicle development. In the first study, mRNA expression levels of housekeeping genes were measured by real-time quantitative PCR (RT-qPCR) in granulosa cells of dominant follicles and FSH-stimulated follicles to select and validate suitable reference genes for relative gene expression analyses during maternal and follicular aging. Stability of six reference genes (GAPDH, ACTB, EIF2B2, UBE2D2, SF3A1 and RNF20) was analyzed using GeNorm, DeltaCT and NormFinder programs and comprehensive ranking order was determined based on these programs. Geometric mean of multiple genes (UBE2D2, EIF2B2, GAPDH and SF3A1) was more appropriate reference control than individual genes for the comparison of ...
Left: The pituitary gonadotropins FSH and LH are part of the hypothalamic-pituitary-gonadal axis that coordinately regulates the menstrual (in humans) or estrous (in nonhuman mammals) cycle by extensive feedback loops. FSH controls follicular granulosa cell (GC) growth and estradiol production, while LH controls ovulation and follicular luteinization. Right: A cross section of a mouse ovary is shown, demonstrating the main cell types and follicle stages. Ovarian follicles are composed of a single oocyte surrounded by somatic cells (granulosa cells) and thecal cells. Follicles grow from primordial (not shown) to primary and secondary stages independent of the pituitary gonadotropins. FSH stimulates growth to the preovulatory follicle stage, characterized by granulosa cells that directly surround the oocyte (cumulus cells) and those that make up the bulk of the wall of the follicle. Following the LH surge, the follicle erupts through the ovarian surface (OSE), and the remaining cells of the ...
Wingless-type mouse mammary tumor virus integration site family (WNT)/β-catenin (CTNNB1) pathway components are expressed in ovarian granulosa cells, direct female gonad development, and are regulated by the pituitary gonadotropins. However, the in vivo functions of CTNNB1 during preovulatory follicular development, ovulation, and luteinization remain unclear. Using a mouse model Ctnnb1(Ex3)fl/fl;Cyp19-Cre (Ctnnb1(Ex3)gc-/-), expressing dominant stable CTNNB1 in granulosa cells of small antral and preovulatory follicles, we show that CTNNB1 facilitates FSH-induced follicular growth and decreases the follicle atresia (granulosa cell apoptosis). At the molecular level, WNT signaling and FSH synergistically promote the expression of genes required for cell proliferation and estrogen biosynthesis, but decrease FOXO1, which negatively regulates proliferation and steroidogenesis. Conversely, dominant stable CTNNB1 represses LH-induced oocyte maturation, ovulation, luteinization, and progesterone ...
Epidermal growth factor (EGF)-like factors [amphiregulin (AREG), betacellulin, and epiregulin] are induced by LH and activate the EGF receptor (ERBB1)/ERK1/2 pathway in granulosa cells and cumulus cells of preovulatory follicles to impact ovulation.
Follicle-stimulating Hormone, Aged, Anti-mullerian Hormone, Body Mass Index, Concentration, Cumulus Granulosa Cells, Environment, Follicular Fluid, Gene, Gene Expression, Gonadotropin, Gonadotropins, Granulosa Cell, Granulosa Cells, Health, Hormone, mRNA, Oocyte, Oocyte Retrieval, Oocytes
Zoological Science publishes articles, reviews and editorials that cover the broad and increasingly interdisciplinary field of zoology.
Follicular selection is the basis for regular ovulation. In the chicken ovary, one small yellow follicle (SYF) is selected daily from a pool of similar size follicles and becomes a preovulatory follicle. Follicle stimulating hormone (FSH) and luteinizing hormone (LH) are primary regulators of follicular growth and differentiation. Epidermal growth factor (EGF), an intraovarian hormone, suppresses granulosa cell differentiation. However, the precise regulation and interactions of these hormones on follicular selection and the expression of their receptors have not been determined. The objectives of this study we (1) develop and validate a suitable culture system for granulosa cells from SYF; (2) investigate if FSH, LH and EGF alone or in combination regulate the abundance of FSH, LH and EGF receptor (FSHr, LHr and EGFr, respectively) and P450 side chain cleavage (P450scc) mRNA and progesterone (P4) production in granulosa cells from SYF; (3) determine the differential abundance of FSHr, LHr, EGFr ...
X-MOL提供的期刊论文更新,Reproductive Biology and Endocrinology--MicroRNA-200b and microRNA-200c are up-regulated in PCOS granulosa cell and inhibit KGN cell proliferation via targeting PTEN,Tingting He; Yifei Sun; Yingchun Zhang; Shigang Zhao; Yanjun Zheng; Guimin Hao; Yuhua Shi
Obstetrics and Gynecology International is a peer-reviewed, Open Access journal that aims to provide a forum for scientists and clinical professionals working in obstetrics and gynecology. The journal publishes original research articles, review articles, and clinical studies related to obstetrics, maternal-fetal medicine, general gynecology, gynecologic oncology, uro-gynecology, reproductive medicine and infertility, reproductive endocrinology, and sexual medicine.
BioMed Research International is a peer-reviewed, Open Access journal that publishes original research articles, review articles, and clinical studies covering a wide range of subjects in life sciences and medicine. The journal is divided into 55 subject areas.
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Einspanier, R.; Pitzel, L.; Wuttke, W.; Hagendorff, G.; Preuss, K. D.; Kardalinou, E.; Scheit, K.H.: Demonstration of mRNAs for oxytocin and prolactin in porcine granulosa and luteal cells. Effects of these hormones on progesterone secretion in vitro. FEBS Letters 204 (1), S. 37 - 40 (1986 ...
In addition, the expression of 12 miRNA families including miR-130 (a, b), bta-miR-181 (a, b, c, d), bta-miR-199 (a-3p, a-5p, b, c), bta-miR-2285 (k, t), bta-miR-2411 (- 3p,-5p), bta-miR-2483 (- 3p,-5p), bta-miR-29 (a, b), bta-miR-339 (a, b), bta-miR-365 (- 3p,-5p), bta-miR-455 (- 3p,-5p), bta-miR-92, and bta-miR-99 (a,-5p, b) were differentially expressed between the granulosa cells of SF and DF (Table 1). [score:5] ...
Novus offers a wide range of antibodies for immunohistochemistry on paraffin sections. View our catalog of IHC-P products plus protocols and tips.
During reproductive life, only a selected few ovarian follicles mature and ovulate, while the vast majority of follicles undergo a degenerative process called atresia. Recent studies have indicated that follicular atresia is mediated through apoptosis of follicular granulosa cells. The objectives of the present study were to determine the time of onset of apoptosis in granulosa cells of preovulatory follicles and to evaluate the consequences of gonadotropin withdrawal on mitogen-activated protein (MAP) kinase activities. Bonnet monkeys (Macaca radiata) undergoing controlled ovarian stimulation cycles were utilized for stimulation of multiple follicles, and granulosa cells were retrieved from preovulatory follicles at 24, 48, 72, and 96 h after stopping gonadotropin treatment. Serum and follicular fluid estradiol concentrations were highest at 24 h but declined precipitously (P , 0.05) to reach the lowest concentrations at 96 h; however, progesterone concentrations during this period did not ...
Tai, C.J.; Kang, S.K.; Tzeng, C.R.; Leung, P.C., 2001: Adenosine triphosphate activates mitogen-activated protein kinase in human granulosa-luteal cells
TY - JOUR. T1 - Colocalization of connexin 43 and connexin 45 but absence of connexin 40 in granulosa cell gap junctions of rat ovary. AU - Okuma, A.. AU - Kuraoka, A.. AU - Iida, H.. AU - Inai, T.. AU - Wasano, K.. AU - Shibata, Y.. PY - 1996/7. Y1 - 1996/7. N2 - The expression and localization of gap junction family proteins (connexins) were examined in nonstimulated and gonadotrophin-stimulated ovarian follicles of immature rats. Immunoblot and RNA blot analysis showed the presence of connexin (Cx) 43, Cx40 and Cx45 in ovarian tissue. Of these connexin proteins, Cx43 and Cx45 were identified by immunofluorescent microscopy between granulosa cells in characteristic expression patterns related to follicular developmental stages, while Cx40 was not expressed in granulosa cells but was detected in blood vessels in ovarian stroma. In some plaques of gap junction between granulosa cells, Cx45 was found to be colocalized with Cx43. In immunofluorescent microscopy, the expression of Cx43 was ...
Oocyte control of granulosa and theca cell function may be mediated by several growth factors via a local feedback loop(s) between these cell types. This study examined both the role of oocyte-secreted factors on granulosa and thecal cells, cultured independently and in co-culture, and the effect of stem cell factor (SCF); a granulosa cell derived peptide that appears to have multiple roles in follicle development. Granulosa and theca cells were isolated from 2-6 mm healthy follicles of mature porcine ovaries and cultured under serum-free conditions, supplemented with: 100 ng/ml LR3 IGF-1, 10 ng/ml insulin, 100 ng/ml testosterone, 0-10 ng/ml SCF, 1 ng/ml FSH (granulosa), 0.01 ng/ml LH (theca) or 1 ng/ml FSH and 0.01 ng/ml LH (co-culture) and with/without oocyte conditioned medium (OCM) or 5 oocytes. Cells were cultured in 96 well plates for 144 h, after which viable cell numbers were determined. Medium was replaced every 48 h and spent medium analysed for steroids.Oocyte secreted factors were ...
TY - JOUR. T1 - Follicle-stimulating hormone increases concentrations of messenger ribonucleic acid encoding cytochrome P450 cholesterol side-chain cleavage enzyme in primary cultures of porcine granulosa cells. AU - Urban, Randall J.. AU - Garmey, James C.. AU - Shupnik, Margaret A.. AU - Veldhuis, Johannes D. PY - 1991/4. Y1 - 1991/4. N2 - FSH is the primary hormonal inducer of ovarian follicle maturation and a critically important regulator of steroidogenesis in granulosa cells. We examined possible molecular mechanisms subserving FSH action by assessing concentrations of cytochrome P450 cholesterol side-chain cleavage (P450scc) mRNA in porcine granulosa cells maintained in serum-free culture. Cellular concentrations of specific P450scc mRNA were measured by Northern blot hybridization using a 32P-labeled 1-kilobase porcine cDNA clone. Specificity was tested by estimating the granulosa cell mRNA content of the constitutively expressed enzyme, glyceraldehyde-3-phosphate dehydrogenase. ...
POF is associated with complex risk factors and high morbidity (2). Typically, studies have focused on the effects of risk factors, including genetic variations, immunity and inflammation, environmental hormones and contamination, personal stress, and endocrine disorders (1,2). During the development of POF, lesions are most prominent in egg and ovarian granulosa cells (4,5,24). Ovarian granulosa cells, similar to Sertoli cells of the male reproduction system, support female reproductive cells i.e. mature egg cells (3), surrounding these cells during follicle development to form a unique microenvironment. Ovarian granulosa cells secrete hormones and cytokines for egg maturation, including estrogen, FSH, anti-Müllerian antibody, inhibinα/β and growth hormone, to support the development, maturation and ovulation of follicles (3). Ovarian granulosa cell apoptosis reduces the follicular reserve capacity of the ovary, thus causing the development of POF (1,5). In the ovaries of POF patients, ...
The contribution of specific follicle populations to dimeric inhibin production and inhibin subunit mRNA expression by the rat ovary has been investigated in two model systems, granulosa cells isolated from 25-day-old diethylstilboestrol (DES)-treated rats and post-natal rat ovaries, dispersed in culture or whole ovaries, using specific two-site immunoassays and real time PCR. Media from FSH-stimulated granulosa cell cultures fractionated by gel filtration and RP-high performance liquid chromatography revealed two predominant peaks of alpha subunit activity which were attributed to alpha subunit and 31 k dimeric inhibin-A. The corresponding inhibin-B levels were low. FSH stimulation did not alter the ratio of inhibin-A:alpha subunit produced by granulosa cells. All three inhibin subunit mRNAs were expressed by granulosa cells, with eight-fold more alpha subunit mRNA relative to either of the beta subunits. Administration of DES to immature rats prior to the isolation of granulosa cells from ...
TY - JOUR. T1 - Adenosine 5′-triphosphate activates nuclear translocation of mitogen-activated protein kinases leading to the induction of early growth response 1 and Raf expression in human granulosa-luteal cells. AU - Tai, Chen Jei. AU - Chang, Shu Ju. AU - Leung, Peter C K. AU - Tzeng, Chii Ruey. PY - 2004/10. Y1 - 2004/10. N2 - With the stimulation of many types of cell surface receptors, MAPKs are activated. We have previously demonstrated an effect of extracellular ATP on ERKs and gonadotropin-induced progesterone secretion, implicating the significance of ATP in the regulation of ovarian function. However, little is known about the specific role of ATP in the subsequent MAPK-induced signaling cascade in human granulosa-luteal cells (hGLCs). The present study was designed to examine the effect of ATP on the activation of the MAPK signaling pathway, including nuclear translocation and the expression of the immediate early genes in hGLCs. Western blot analysis, using a monoclonal antibody, ...
This study was designed to develop preantral follicle isolation and classification protocols for the domestic dog as a model for endangered canids. Ovary donors were grouped by age, size, breed purity, ovary weight and ovary status. Ovaries were randomly assigned to 1 of 3 digestion protocols: A) digestion and follicle isolation on the day of spaying; B) storage at 4 degrees C for 18 to 24 h prior to digestion and follicle isolation; C) digestion on the day of spaying, then incubation at 4 degrees C for 18 h prior to follicle isolation. Minced tissue was placed in a collagenase/DNase solution at 37 degrees C for 1 h. Follicles were classified by oocyte size and opaqueness and by size and appearance of the granulosa cell layers. Preantral follicles contained small, pale oocytes. Preantral follicles containing grown oocytes with dense cytoplasmic lipid were designated as advanced preantral. Only advanced preantral and early antral follicles were examined and classified further. Group 1 follicles had
The human LH receptor (LHR) plays a key role in luteal function and the establishment of pregnancy through its interaction with the gonadotropins LH and human chorionic gonadotropin. We previously identified four splice variants of the LHR in human luteinized granulosa cells (LGCs) and corpora lutea (CL). Real-time quantitative PCR revealed that expression of the full-length LHR (LHRa) and the most truncated form (LHRd) changed significantly in CL harvested at different stages of the ovarian cycle (P , 0.01, ANOVA). LHRa expression was reduced in the late luteal CL (P,0.05). Conversely, an increase in LHRd expression was observed in the late luteal CL (P,0.01). Chronic manipulation of human chorionic gonadotropin in LGC primary cultures supported the in vivo findings. LHRd encodes a protein lacking the transmembrane and carboxyl terminal domains. COS-7 cells expressing LHRd were unable to produce cAMP in response to LH stimulation. COS-7 cells coexpressing LHRd and LHRa also failed to generate ...
Our proposal is to conduct an open phase II clinical trial that allows us to explore the activity of ketoconazole, an inhibitor of the enzyme CYP17, in ovarian granulosa tumors similar to what has been done in prostate cancer. The rational is based on dysregulation that FOXL2 mutations present in almost all granulosa tumors result in the expression of CYP17 that appears to be key in the development and progression of the disease.. This work would represent the first attempt to address the treatment of ovarian granulosa cancer with a molecular solid rational, drawing on the recent identification of the mutation leader of this tumor. If succeed provide a widely available therapeutic alternative compared with current cancer therapies, with low toxicity. In addition it would open a new line of research with CYP17 enzyme inhibitors that could alter the course and outcome, usually fatal, in advanced stages of disease. ...
Inhibin and Activin are important local modulators of theca cell steroidogenesis in the ovary. needed account activation of the Smad2/3 path. Activin reduced the phrase amounts of steroidogenic severe regulatory proteins (Superstar), whereas Superstar phrase was elevated by SB-431542 and inhibin, by itself and in mixture. was untouched. The phrase of coding 17-hydroxylase was untouched by activin but elevated by SB-431542 and inhibin, and when added in mixture the impact was enhanced further. The phrase of 3-hydroxysteroid dehydrogenase (and by phrase of aromatase in the cumulus granulosa cells encircling the oocyte in the preovulatory hair foillicle, hence reducing exposure to these damaging effects of excess testo-sterone and androgens possibly. In rats, androgens not really just can induce follicular atresia (Hillier & Ross 1979, Daniel & Armstrong 1986, Billig for 5?minutes and resuspended in lifestyle mass media (DMEM-F12 with 100?kIU/d penicillin, 01?g/d streptomycin, 3?mmol/d l-glutamine, ...
Inhibin and Activin are important local modulators of theca cell steroidogenesis in the ovary. needed account activation of the Smad2/3 path. Activin reduced the phrase amounts of steroidogenic severe regulatory proteins (Superstar), whereas Superstar phrase was elevated by SB-431542 and inhibin, by itself and in mixture. was untouched. The phrase of coding 17-hydroxylase was untouched by activin but elevated by SB-431542 and inhibin, and when added in mixture the impact was enhanced further. The phrase of 3-hydroxysteroid dehydrogenase (and by phrase of aromatase in the cumulus granulosa cells encircling the oocyte in the preovulatory hair foillicle, hence reducing exposure to these damaging effects of excess testo-sterone and androgens possibly. In rats, androgens not really just can induce follicular atresia (Hillier & Ross 1979, Daniel & Armstrong 1986, Billig for 5?minutes and resuspended in lifestyle mass media (DMEM-F12 with 100?kIU/d penicillin, 01?g/d streptomycin, 3?mmol/d l-glutamine, ...
Ovarian follicle is a fluid filled structure that consists of the developing oocyte as well as other cells involved in the menstuation process -Every month one of these primary follices containing a primary oocyte will devleop into a secondary follicle that contains a secondary oocyte. -During stimulation, secondary oocyte is released into the fallopian tubes while the remainder of cells develop into the corpus luteum.. -Other than the oocyte itself, there are two types of cells in the ovarian follicle:. (1) Theca Cell: Regulates Oogenesis and produces sex steroid hormones. (2) Granulosa Cell: FSH stimulates granulosa cells to convert androgens (coming from the thecal cells) to estradiol by aromatase during the follicular phase of the menstrual cycle.. General Process: LH acts on -, Theca cell = Cholesterol -, Androgen -, Travels to Granulosa cell; which with the aid of FSH; Androgen -, Oestrogen.. ...
Granulosa cell tumors (GCT) of the ovary are relatively uncommon (less than 5% of all ovarian malignancies). GCTs can be divided on the basis of histopathological features into juvenile and adult types; these designations reflect the usual age group in which each type occurs. Adult-type GCTs (A-GCT) are most frequently diagnosed in perimenopausal women, at a mean age that is 10 years younger than that of women diagnosed with epithelial ovarian cancer. Patients with A-GCT usually present with low stage disease and have a relatively favorable prognosis, yet the risk of recurrence is unpredictable, and may occur 10 to 30 years after initial diagnosis. For women with recurrent disease or advanced stage tumors at diagnosis, there is no effective treatment beyond surgery, and 20 to 30% of women diagnosed with A-GCT will ultimately die of their disease (1).. Past efforts to understand the molecular pathogenesis of GCTs have been limited by their relative rarity. Since pre-ovulatory growth of normal ...
I am, a medical practitioner, interested in attending the next Granulosa Cell Tumor Conference and would love to visit New Zealand if that is where it may be held.
Primordial follicles are found just under the tunica albuginea. They are one cell thick layer of somatic cells wrapping one oocyte, which is arrested in prophase I. Somatic cells area rather flattened to cuboidal, in close apposition to the oocyte. The follicle is separated from the surrounding stroma by a basal lamina. Those follicles that start growing also begin to mature, and will eventually release the fully developed ovum during ovulation, at the end of this maturing process. Depending on the author and the developing stage, follicles receive different names. Primary follicles show an increase in the oocyte size and the follicle somatic cells covering the oocyte change from flattened to cuboidal shapes. Furthermore, the pellucida membrane starts to be visible between the oocyte and the somatic cells of the follicle. As maturation progresses, follicle somatic cells proliferate and reorganize into several layers, altogether known as granulosa cells. Granulosa cells are enclosed by the ...
Effect of glucose treatment on basal and FSH- or IGF-1-stimulated progesterone and oestradiol secretions by rat granulosa cells. Granulosa cells from immature r
Interspecies-SCNT preimplantation embryos derived from human granulosa cells fused with enucleated bovine oocytes. Cleavage embryos (A) and blastocysts (C) derived from SCNT. Parthenogenetically developed cleavage embryos (B) and hatching blastocysts (D) as controls ...
Definition of Membrana hyaloidea with photos and pictures, translations, sample usage, and additional links for more information.
Frost, Darrel R. 2014. Amphibian Species of the World: an Online Reference. Version 6.0. Electronic Database accessible at http://research.amnh.org/herpetology/amphibia/index.html. American Museum of Natural History, New York, USA. Cochranella granulosa . Accessed on 19 May 2008 ...
This study assessed the effects of 4-nitrophenol (PNP) and 3-methyl-4-nitrophenol (PNMC) on steroidogenesis in the granulosa layers (GLs) and theca layers (TLs) of chicken preovulatory follicles in vitro and in vivo. In the.... Read More ...
Dr. Burak Hacıhanefioğlu 1969 yılında İstanbulda doğdu. 1986 yılında girdiği Hacettepe Üniversitesi Tıp Fakültesinden 1993 yılında mezun oldu. 1993-1999 yılları arasında İstanbul Üniversitesi Cerrahpaşa Tıp Fakültesinde Kadın Hastalıkları ve Doğum ihtisası yaptı. Kadın Hastalıkları ve Doğum ihtisası döneminde Polikistik Ovaryum Sendromunda İnsülin Direnci ve Renin-Angiotensin sistemi (RAS) arasındaki ilişkinin değerlendirilmesi konulu tezini tamamladı.. Dr. Burak Hacıhanefioğlu Kadın Hastalıkları ve Doğum ihtisasını bitirdikten sonra sonra 1999 yılında İsveçte Orebro Üniversitesinde polikistik over sendromunda yumurta gelişimi ve yumurtlama konusunda çalışmalarda bulundu.. 2000-2002 yılları arasında İngilterede Newham General Hospitalda Üreme Endokrinolojisi bölümünde araştırma görevlisi olarak çalıştı. Bu dönemde Investigation of apoptotic and cell cycle pathways in granulosa cells of women with polycystic ...
O presente trabalho teve como objetivo estudar o processo de microfiltração tangencial com membranas tubulares cerâmicas, aplicado à clarificação da vinhaça originária do processamento de cana de açúcar,...
Neste estudo foram avaliados os efeitos antitumorais da fosfoetanolamina sintética (FS) em modelos de tumores experimentais e as vias de sinalizações envolvidas nesta atividade. In vitro a FS foi citotóxica...
BACKGROUND: In mammalian follicles, oocytes are arrested at the diplotene stage of prophase I until meiotic resumption following the LH surge. Recently, C-type natriuretic peptide (CNP), encoded by natriuretic peptide precursor type C (NPPC) was found to suppress mouse oocyte maturation by promoting cyclic guanosine 5-monophospate (cGMP) production in cumulus cells. However, regulation of NPPC/CNP expression during the pre-ovulatory period and their regulation by the LH surge have not been investigated.. METHODS AND RESULTS: Based on genome-wide analysis of DNA microarray data sets using samples from periovulatory ovaries, we found increases in NPPC transcripts in granulosa cells during pre-ovulatory follicle growth in mice and a rapid decline induced by the pre-ovulatory LH/hCG stimulation. Treatment of pre-ovulatory animals with hCG decreased ovarian CNP content. In isolated ovarian cells, NPPC mRNA was predominantly expressed in mural granulosa cells exhibiting similar regulation following ...