Anaerococcus is a genus of bacteria. Its type species is Anaerococcus prevotii. These bacteria are Gram-positive, anaerobic, indole-negative cocci. Its genome was sequenced in August 2009. It can cause infection and is a normal part of the human microbiome. It is a pathogen of humans found in ovarian abscesses, chronic wounds and vaginal discharge. It can be present in urinary tract infections, chronic ulcers, pleural empyema, blood infections, and soft tissue infections. It is involved in polymicrobial infections. Anaerococcus senegalensis Anaerococcus murdochii Anaerococcus vaginalis Anaerococcus tetradius Anaerococcus prevotii Anaerococcus octavius Anaerococcus lactolyticus Parte, A.C. Anaerococcus. Retrieved 27 March 2017. BacMap. Retrieved 27 March 2017. Murphy, Elizabeth Carmel; Frick, Inga-Maria (2013). Gram-positive anaerobic cocci - commensals and opportunistic pathogens. FEMS Microbiology Reviews. 37 (4): 520-553. doi:10.1111/1574-6976.12005. ISSN 1574-6976 ...
Looking for Gram-positive cocci? Find out information about Gram-positive cocci. A form of eubacteria which are more or less spherical in shape Explanation of Gram-positive cocci
Beswick, Alan J; Lawley, Blair; Fraise, Adam P; Pahor, Ahmes L; Brown, Nigel L (1999). Detection of Alloiococcus otitis in mixed bacterial populations from middle-ear effusions of patients with otitis media. The Lancet. 354 (9176): 386-389. doi:10.1016/S0140-6736(98)09295-2. ISSN 0140-6736. PMID 10437868 ...
Two strains of a Gram-positive catalase-negative, facultatively anaerobic coccus originating from human sources were characterized by phenotypic and molecular taxonomic methods. The strains were found to be identical to each other based on 16S rRNA gene sequencing and constitute a new subline within the genus Facklamia. The unknown bacterium was readily distinguished from Facklamis hominis and Facklamia ignava by biochemical tests and electrophoretic analysis of whole-cell proteins. Based on phylogenetic and phenotypic evidence it is proposed that the unknown bacterium be classified as Facklamia sourekii sp. nov., the type strain of which is CCUG 28783AT.
Conclusions These findings indicate that linezolid has an excellent activity against clinical drug resistant Gram-positive isolates in Iran. Constant monitoring and surveillance of linezolid MIC distribution allows the researchers to assess and detect gradual upward MIC drifts....
Cocci bacteria are bacteria that are spherical or oval in shape. The three basic shapes of bacteria are coccus, bacillus and spiral. Cocci bacteria can form arrangements that have different...
microbiology result UNKNOWN INSTRUCTIONS DAY 1: Today you will be given a broth tube containing two unknown microorganisms: a Gram-positive coccus and a Gram-negative rod.…
Study Flashcards On Buxton Gram Positive Cocci 4/18 at Cram.com. Quickly memorize the terms, phrases and much more. Cram.com makes it easy to get the grade you want!
Your professor should have given you a dichotomous key or some other type of table(s) to help you identify your bacteria. At the very least I would think a list of bacteria would be provided, because only the professor knows what bacteria the students were given. When I had my unknown project in micro we were provided such information; I couldnt imagine not having it! Sorry I cant help you further, good luck ...
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Dps (DNA protection during starvation) proteins play an important role in the protection of prokaryotic macromolecules from damage by reactive oxygen species. The Dps homolog, Dps-1, from the radiation-resistant bacterium Deinococcus radiodurans has an extended N-terminal tail. In the crystal structure of Dps-1, the first ~30 N-terminal residues are invisible and the remaining 25 residues form a loop that harbors a novel metal binding site. The data presented here show that retention of this N-terminal metal site is necessary for formation of the dodecameric protein assembly. Previous studies have suggested that the lysine-rich N-terminus of Dps proteins participates in DNA binding. Accordingly, deletion of the N-terminal tail of Dps-1 obliterates DNA/Dps-1 interaction. Electrophoretic mobility shift assays using DNA modified with specific major/minor groove reagents show that Dps-1 interacts through the DNA major groove. Dodecameric Dps-1 can bind ? 22bp DNA duplexes with very high affinity (Kd ...
Oenococcus oeni temperate bacteriophage fOg44 Lys protein: from Oenococcus oeni bacteriophage fOg44 and lactobaccilius phage phig1e; amino acid sequence in first source; GenBank AF047001 (phage fOg44)
My laboratory studies the structure and function of protein-DNA complexes required for the initiation and movement of DNA replication forks, for homologous recombination, and for DNA repair processes including double-strand break repair (DSBR). Our model systems include the bacteriophage T4, the extremely radiation-resistant bacterium Deinococcus radiodurans, the budding yeast Saccharomyces cerevisiae, and humans. Our experimental approaches include steady-state and pre-steady-state kinetics, fluorescence spectroscopy, site-directed mutagenesis, single-molecule enzmology, X-ray crystallography, and others. Current projects in the lab include: Mechanisms of DNA helicase assembly and translocation at DNA replication forks. DNA helicase enzymes unwind double-stranded DNA at replication forks, allowing leading and lagging strand DNA synthesis to occur. Studies of two DNA helicases, Gp41 and Dda, in the bacteriophage T4 replication system have provided important insights on the roles of helicase in ...
Two gamma- and UVC-resistant bacterial strains, designated JSH3-1T and 9-2-2, were isolated from garden soil in South Korea. Cells were Gram-stain-positive, aerobic, non-motile and spherical. A polyphasic approach was used to study the taxonomic properties of strains JSH3-1T and 9-2-2. Phylogenetic analysis based on nearly full-length 16S rRNA gene sequences of strains JSH3-1T and 9-2-2 indicated highest similarity with Deinococcus radiomollis PO-04-20-132T (94.7 and 94.9 %, respectively); levels of sequence similarity with the type strains of other Deinococcus species were less than 94.0 %. Strains JSH3-1T and 9-2-2 shared relatively high 16S rRNA gene sequence similarity (98.7 %) and had a high DNA reassociation value of 81±0.5 %. Meanwhile, they showed low levels of DNA reassociation (<25 %) with other closely related species of the genus Deinococcus . The two strains showed chemotaxonomic features typical of the genus Deinococcus , with the presence of menaquinone 8 as the respiratory
Crystalline surface layer proteins (S-layer proteins) have considerable potential for the crystalline arrays in biotechnology, biomimetics and nonlife applications, including areas such as microelectronics and molecular nanotechnology. The extensive application potential of surface layers in nanobiotechnology is according to the particular inherent attributes of the single molecular arrays consisted of uniform protein or glycoprotein subunits. Most important, functional groups on the protein lattice are arrayed in well-specified positions and orientations. Many applications of S-layers are related to the ability of isolated subunits to recrystallize into single molecular arrays in suspension, suitable surfaces or interfaces. Utilization of the s-layers as template to pattern inorganic nanostructures, requires the separation and purification of these proteins and study of their structures on solid surfaces. The hexagonally packed intermediate (Hpi) protein of Deinococcus radiodurans belongs to the
The measured m/z value for the [M+Na]+ ion of DR8 was 1,521.6, very close to the mass calculated for a cross-linked dimer without one disaccharide moiety (1,520.53) (Fig. 1; Table 1). Such muropeptides, also identified in T. thermophilus HB8 and other bacteria (18, 19), are most likely generated by the enzymatic clevage of MurNAc-l-Ala amide bonds in murein by an N -acetylmuramyl-l-alanine amidase (21). In particular, DR8 could derive from DR11. The difference between measured m/z values for DR8 and DR11 was 478.7, which fits with the mass contribution of a disaccharide moiety (480.5) within the mass accuracy of the instrument.. The m/z values for muropeptides DR2, DR10, and DR12 supported the argument for structures in which the twod-Ala residues from thed-Ala-d-Ala C-terminal dipeptide were lost, leaving Orn as the C-terminal amino acid.. The position of one Gly residue in muropeptides DR2, DR8, and DR10 to DR13 could not be formally demonstrated. One of the Gly residues could be at either the ...
Su tiempo de generación se mide en aproximadamente 80 minutos en condiciones de crecimiento óptimas. El mejor crecimiento se ve en entre 25 a 35 ° C sin embargo cesa el crecimiento por debajo de 4 ° C y por encima de 45 ° ...
Bacteria of the genus Deinococcus are extremely resistant to ionizing radiation (IR), ultraviolet light (UV) and desiccation. The mesophile Deinococcus radiodurans was the first member of this group whose genome was completely sequenced. Analysis of the genome sequence of D. radiodurans, however, failed to identify unique DNA repair systems. To further delineate the genes underlying the resistance phenotypes, we report the whole-genome sequence of a second Deinococcus species, the thermophile Deinococcus geothermalis, which at itsoptimal growth temperature is as resistant to IR, UV and desiccation as D. radiodurans, and a comparative analysis of the two Deinococcus genomes. Many D. radiodurans genes previously implicated in resistance, but for which no sensitive phenotype was observed upon disruption, are absent in D. geothermalis. In contrast, most D. radiodurans genes whose mutants displayed a radiation-sensitive phenotype in D. radiodurans are conserved in D. geothermalis. Supporting the existence of
Although more than 80% of infected and 70% of noninfected leg ulcers have been shown to harbor anaerobic organisms, their role in mediating impaired wound healing in the skin is frequently overlooked. There is now increasing evidence that the gram-positive anaerobic cocci play a role (both directly and indirectly) in mediating impaired wound healing in vivo. This article discusses the mechanisms by which these microorganisms may interfere with the inflammation, repair, and remodeling phases of the wound healing process.. ...
Nemonoxacin (Taigexyn®): A New Non-Fluorinated Quinolone. By Li-Wen Chang, Ming-Chu Hsu and Ying-Yuan Zhang. Nemonoxacin (Taigexyn®), a novel C-8-methoxy non-fluorinated quinolone, has been approved for use in community-acquired pneumonia (CAP) in Taiwan (2014) and mainland China (2016). The FDA granted nemonoxacin qualified infectious disease product and fast-track designations for CAP and acute bacterial skin and skin structure infection in December 2013. It possesses a broad spectrum of bactericidal activity against typical and atypical respiratory pathogens. In particular, nemonoxacin has activity against resistant Gram-positive cocci, including penicillin-resistant Streptococcus pneumoniae and methicillin-resistant Staphylococcus aureus. Oral nemonoxacin was compared with oral levofloxacin for efficacy and safety in three randomized, double-blinded, controlled Phase II-III clinical trials for the treatment of CAP. This article will review the microbiological profile of nemonoxacin ...
Japan Geoscience Union Meeting 2018,Mutation analysis of the <em>rpoB</em> gene in the radiation-resistant bacterium, <em>Deinococcus radiodurans</em> R1 exposed to space
Formerly a member of the streptococcaceae, the enterococci now belong to their own bacterial family. Their morphology is quite similar to that of Streptococcus. Both are gram + cocci, however, but the enterococci are slightly elongated (oval) and are aggregated in shorter chains when viewed under the microscope. As the name implies, the enterococci are members of the normal gastrointestinal flora. The clinically most important species are E. faecalis and E. faecium. They are categorized as facultative pathogens as they do not cause any harm if contained within the gut lumen. However, if they are able to penetrate through the gut wall or are displaced to other mucosal membranes they can cause severe infections. The enterococci can penetrate the gut wall through lesions/ulcers or due to decreased immune presence and displacement is mostly due to fecal contamination due to inadequate hygiene. Once outside the gut, they can cause infections such as UTIs, cystitis, peritonitis, and bacteremia, which ...
Deinococcus radiodurans ATCC ® 13939D-5™ Designation: Genomic DNA from Deinococcus radiodurans strain R1 (smooth) TypeStrain=True Application:
There are also plenty of simpler creatures that can survive in extreme conditions. For example, the bacterium deinococcus radiodurans can repair its DNA on the fly, withstanding doses thousands of times higher than the lethal limit for humans. For the zerg to survive long in space, they would need to share this ability to repair radiation damage rapidly.. They would also have to be able to survive without air, and to completely exhale all the air inside them safely before entering space. One possible mechanism to help the zerg survive without air is the creep. Creep provides sustenance to zerg buildings, but if it also could nourish zerg creatures, it might keep them going without the need to breathe. Combine that with a slow metabolism, making any nourishment or oxygen absorbed from the creep last for a while after they leave it, and you might be able to do away with the need to breathe. This is admittedly far-fetched, and would work much better for microscopic creatures with higher surface ...
Anaerococcus senegalensis strain JC48T (= CSUR P156 = DSM25366) is the type strain of A. senegalensis sp. nov. This bacterium is a Gram-positive, anaerobic, indole-negative coccus that was isolated from the stool of a healthy Senegalese patient as part of a culturomics study aimed at cultivating individually all species within human feces.. Defining bacterial species is a matter of debate. This is notably due to the elevated cost and poor reproducibility and inter-laboratory comparability of the gold standard DNA-DNA hybridization and G+C content determination [1]. In contrast, the development of PCR and sequencing methods is now widely available and cost-effective, which profoundly changes the way Archaea, Bacteria and are classified. Using 16S rRNA sequences with internationally-validated cutoff values enabled the taxonomic classification or reclassification of hundreds of taxa [2]. More recently, high throughput genome sequencing and mass spectrometric analyses of bacteria gave ...
DNA protection during starvation (Dps) proteins are important for bacterial oxidative stress responses. This study aims to understand the roles and characteristics of the two Dps homologs in Deinococcus radiodurans: Dps-1 and Dps-2. Dps-2 contains a predicted signal peptide and in vivo localization of Dps-2 reveals that its location is non-cytoplasmic. β-galactosidase assays show that the Dps-2 promoter is upregulated in the presence of H2O2 and the results from the DNA protection assay show that Dps-2 is able to protect DNA efficiently against reactive oxygen species (ROS). Dps-2 has a C-terminal extension that is needed for assembly into a dodecamer but not for DNA binding. Dps-1 is assembled from six dimers and stoichiometric experiments reveal that the stoichiometry of Dps-1 binding to 22 bp DNA substrate is 1:6, meaning that Dps-1has six DNA binding sites. However, for the 26 bp DNA substrate, the stoichiometry is 1:4, suggesting that the protein can interact with both faces of a DNA duplex
The primary use of antibiotics in pharyngitis is to treat infection due to group A Streptococcus (GAS). GAS is the most common bacterial agent causing acute pharyngitis, and accounts for approximately 15 to 30 of cases in children and 5 to 10 of adults (44,45). However, streptococcal pharyngitis is difficult to differentiate from other causes (such as viral etiology) on clinical grounds (45). Definitive diagnosis of streptococcal pharyngitis is based on the identification of GAS in the throat.... ...
Cirioni, O., Myszka, H., Dawgul, M., Ghiselli, R., Orlando, F., Silvestri, C., Brescini, L., Kamysz, W., Guerrieri, M., Giacometti A. In vitro activity and in vivo efficacy of the saponin diosgenyl 2-amino-2-deoxy-.-D-glucopyranoside hydrochloride (HSM1) alone and in combination with daptomycin and vancomycin against Gram-positive cocci J. Med. Microbiol., 60 (2011) 1337-1343, IF 2, ...
In vitro activity and in vivo efficacy of the saponin diosgenyl 2-amino-2-deoxy-β-D-glucopyranoside hydrochloride (HSM1) alone and in combination with daptomycin and vancomycin against Gram-positive cocci. ...
TYTU : In vitro activity and in vivo efficacy of the saponin diosgenyl 2-amino-2-deoxy-β-D-glucopyranoside hydrochloride (HSM1) alone and in combination with daptomycin and vancomycin against Gram-positive cocci ...
Cipro is an effective antibiotic that treats a wide variety of infections; however, its use is restricted in children and it carries a risk of tendinitis or tendon rupture. Keflex is a cephalosporin-type antibiotic that may be used in the treatment of gram-positive cocci infections.
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Protists are any various one-celled organisms that are free-living or organized into simple colonies. Protists may include protozoans, eukaryotic algae, and slime molds. This slide is a smear of of three typical bacteria types: cocci, bacilli, and spirilli. PRODUCT WARNINGS Our products are not toys. Use in a laborator
Study L16_Cocci flashcards from Michael Canfield's class online, or in Brainscape's iPhone or Android app. ✓ Learn faster with spaced repetition.
p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class=publication>Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href=http://www.nrbook.com/b/bookcpdf.php>Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...
Vagococcus penaei is a species of bacteria. It is Gram-positive, catalase-negative and coccus-shaped. Its type strain is CD276T (=LMG 24833T =CIP 109914T). (en) ...
My second week in S-STEM was intriguing. I started to get used to the scholars, I began to locate lab equipments by myself, and I also stopped getting lost in the laboratories, which is a thumb up for me. This week was all about finding out my Unknown bacteria. After being provided my Unknown bacteria, figuring out what type (gram positive or gram negative), shape (Cocci, Bacilli), and the name of bacteria was my job to find out. There are multiple steps in finding out your Unknown bacteria. Below are the steps I used to demonstrate mine. ...
Lineage: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Tissierellia; Tissierellales; Peptoniphilaceae; Finegoldia; Finegoldia ...
A smear from a simulated joint fluid collected from a 5 year old female with severe sepsis was sent to category A, B, C, and C1 laboratories for Gram staining.. Participants were expected to report the presence of gram positive cocci and neutrophils.. ...
5S rRNA sequences were determined for the myxobacteria Cystobacter fuscus, Myxococcus coralloides, Sorangium cellulosum, and Nannocystis exedens and for the radioresistant bacteria Deinococcus radiodurans and Deinococcus radiophilus. A dendrogram was constructed by using weighted pairwise grouping based on these and all other previously known eubacterial 5S rRNA sequences, and this dendrogram showed differences as well as similarities compared with results derived from 16S rRNA analyses. In the dendrogram, Deinococcus 5S rRNA sequences clustered with 5S rRNA sequences of the genus Thermus, as suggested by the results of 16S rRNA analyses. However, in contrast to the 16S rRNA results, the Deinococcus-Thermus cluster divided the 5S rRNA sequences of the alpha subdivision of the class Proteobacteria from the 5S rRNA sequences of the beta and gamma subgroups of the Proteobacteria. The myxobacterial 5S rRNA sequence data failed to confirm the existence of a delta subgroup of the class Proteobacteria, which
Cellobiose versus glucose utilization by the ruminal bacterium Ruminococcus albus. Isolation and characterization of Selenomonas ruminantium strains capable of 2-deoxyribose utilization
A monoclonal antibody (MAb S1) generated against Exoglucanase A was used to investigate cellulolysis by R. flavefaciens. MAb S1 recognized few components in crude cellulase demonstrating its specificity for Exoglucanase A. MAb S1 was used to investigate the cellular location of the Exoglucanase A. Thin-sectioning of R. flavefaciens followed by immunolabelling, and examination using a transmission electron microscope showed a non-specific interaction of the monoclonal antibody for the embedding resin. Although, various treatments were tried to prevent this interaction, no conclusions about the cellular location of Exoglucanase A could be drawn. MAb S1 also inhibited the action of Exoglucanase A in vitro but failed to inhibit growth of R. flavefaciens when added to culture medium. This suggests the action of Exoglucanase A is not necessary for growth or the antibody concentration in the medium was too low to affect growth ...
A new spanish study performed in collaboration with technical institutes investigates the effects of co-inoculation ( inoculation of wine bacteria 24 hours after yeast addition) with two different wine bacteria Oenococcus oeni (PN4 and OMEGA under MBR form , from Lallemand oenology) on the kinetics of malolactic fermentation (MLF), wine chemical composition, and organoleptic characteristics in comparison with spontaneous MLF in Tempranillo grape must from Castilla-La Mancha (Spain).
A second category of proteins was involved in converting components of grapes and yeast to the fruity, nutty, buttery, other flavors in wine.. A third category of proteins was involved in making alcohol, although this is primarily the function of the yeast.. Finally, they compared the identified proteins from this microbe with those of other similar microbes.. This work sheds additional light on the complex process of making wine, and may eventually be useful in helping vintners select the best bacterial strains to produce the desired odors and flavors in wine.. It also provides some appreciation of the complex process involved in converting grapes into the nectar of the gods.. More information is available in the article by María de la Luz Mohedano entitled A partial proteome reference map of the wine lactic acid bacterium Oenococcus oeni ATCC BAA-1163. in Open Biol. 2014 4, 130154, published 26 February 2014 ...
Anaerococcus prevotii ATCC ® 9321D-5™ Designation: Genomic DNA from Anaerococcus prevotii strain PC 1 TypeStrain=True Application:
penicillinsを復習基礎力を固めたいと思います。UpToDate より引用Penicillin G is highly active against most Gram-positive cocci, Gram-positive rods, Gram-negative cocci, and anaerobes.…
Columbia CNA Agar is recommended for use as a selective growth medium for the isolation and differentiation of gram-positive cocci from clinical and non-clinical specimens which contain mixed flora. ...
2nd May 2015 The vine growers believe that the land on which they grow vines gives the wines a unique quality, and that is called terroir. We can consider that the physiological response of the vines to the type of soil and climatic conditions, together with the characteristics of the variety and form of cultivation,…
As a member of the wwPDB, the RCSB PDB curates and annotates PDB data according to agreed upon standards. The RCSB PDB also provides a variety of tools and resources. Users can perform simple and advanced searches based on annotations relating to sequence, structure and function. These molecules are visualized, downloaded, and analyzed by users who range from students to specialized scientists.
Any ideas if theres something wrong? My sweet little chick is acting extra tired today. It just started a few hours ago. All the other chicks are running...
Modules of 290 residues appended to GH43_16 enzymes. So far found exclusively in Ruminococci. The xyloglucan-binding function was demonstrated for the R. flavefaciens protein ...