TY - JOUR. T1 - Influence of referral bias on the clinical characteristics of patients with Gram-negative bloodstream infection. AU - Al-Hasan, M. N.. AU - Eckel-Passow, J. E.. AU - Baddour, L. M.. PY - 2011/11. Y1 - 2011/11. N2 - Referral bias can influence the results of studies performed at tertiary-care centres. In this study, we evaluated demographic and microbiological factors that influenced referral of patients with Gram-negative bloodstream infection (BSI). We identified 2919 and 846 unique patients with Gram-negative BSI in a referral cohort of patients treated at Mayo Clinic Hospitals and a population-based cohort of Olmsted County, Minnesota, residents between 1 January 1998 and 31 December 2007, respectively. Multivariable logistic regression analysis was used to determine factors associated with referral. Elderly patients aged ≥80 years with Gram-negative BSI were less likely to be referred than younger patients [odds ratio (OR) 0·43, 95% confidence intervals (CI) 0·30 - 0·62] ...
Multidrug-resistant gram-negative bacilli are emerging threats in the intensive care unit setting worldwide. Extended-spectrum β-lactamases, AmpC β-lactamases, and carbapenem-resistant Enterobacteriaceae are increasing at an alarming rate, leaving limited therapeutic options. In addition, multidrug resistance among Pseudomonas aeruginosa and Acinetobacter baumannii has widely disseminated and become a frequent cause of nosocomial infections within many intensive care units. Therefore, resistance is increasing to all currently available antibiotics, including cephalosporins, penicillins, aztreonam, carbapenems, fluoroquinolones, and aminoglycosides. Some multidrug-resistant gram-negative bacteria remain susceptible to only a few antibiotics such as tigecycline, fosfomycin, and polymyxins. The steady trend of increasing resistance coupled with the lack of novel antibiotics targeting resistant gram-negative bacilli has forced clinicians to increasingly apply more aggressive dosing strategies, ...
Gram-negative bacterial infection refers to a disease caused by gram-negative bacteria. One example is E. coli. It is important to recognize that this class is defined morphologically (by the presence of a bacterial outer membrane), and not histologically (by a pink appearance when stained), though the two usually coincide. One reason for this division is that the outer membrane is of major clinical significance: it can play a role in the reduced effectiveness of certain antibiotics, and it is the source of endotoxin. The gram status of some organisms is complex or disputed: Mycoplasma are sometimes considered gram-negative, but because of its lack of a cell wall and unusual membrane composition, it is sometimes considered separately from other gram-negative bacteria. Gardnerella is often considered gram-negative, but it is classified in MeSH as both gram-positive and gram-negative. It has some traits of gram-positive bacteria, but has a gram-negative appearance. It has been described as a ...
Gram-Negative Bacterial Infections; Bacterial Infections, Gram-Negative. On-line free medical diagnosis assistant. Ranked list of possible diseases from either several symptoms or a full patient history. A similarity measure between symptoms and diseases is provided.
BioAssay record AID 558745 submitted by ChEMBL: Clearance of formed compound in patient with gram-negative bacterial infection at 250 mg/kg, iv.
The research report offers insights into both existing and therapeutics under development for the treatment of gram-negative bacterial infection.
Spero Therapeutics antibiotic pipeline is focused on areas for which the unmet medical need is greatest, the biological rationale for treatment is clear, and for which there are few approved therapies, or where the standard of care is suboptimal.. Our pipeline of anti-infective agents is one of the most unique in the industry and consists of three investigational product candidates, including:. ...
Papers and discussions from a meeting held in Vienna, September 1973. Most of the papers will be of interest mainly to investigators of the nature and pathogenetic mechanisms of endotoxin. The seventh and eighth sections include, however, papers on important clinical problems precipitated by Gram-negative bacterial endotoxemia: coagulation disorders, shock, and pulmonary effects. Detailed subject index. ...
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Lyme disease has emerged as an increasing problem for people in the east and northeastern part of the United States. It can cause a chronic debilitating infection if left untreated and is difficult to diagnose. The illness ...
Build: Wed Jun 21 18:33:50 EDT 2017 (commit: 4a3b2dc). National Center for Advancing Translational Sciences (NCATS), 6701 Democracy Boulevard, Bethesda MD 20892-4874 • 301-435-0888. ...
Free flashcards to help memorize facts about Gram Negative Bacilli. Other activities to help include hangman, crossword, word scramble, games, matching, quizes, and tests.
Garenoxacin mesylate [USAN] is a novel oral des-fluoro quinolone with potent antimicrobial activity against common respiratory pathogens, including resistant strains and Gram-positive and Gram-negative bacterial infections.
Canada/Ottawa: Les Oeufs Richard Eggs Inc., a Rivière-Héva, Quebec establishment, recalls certain batch/lots of Les Oeufs Richard Eggs, Nutri and No Name branded Fresh Chicken Eggs from the Canadian marketplace due to suspected Salmonella contamination and consequential risks of Gram-negative bacterial infection, severe illness and/or possible life-changing complications, all serious health hazards. CFIA: http://ht.ly/aRs230rlrOb Direct…
A collection of disease information resources and questions answered by our Genetic and Rare Diseases Information Specialists for Stenotrophomonas maltophilia infection
What does this mean and reasons for caution?. There were several important observations drawn from this study. The first is that children with CF were found to be at increased risk of acquiring S. maltophilia infection, contrary to previous beliefs that this bacterium was more of a problem in older patients with CF and end stage lung disease. In addition, there has previously been a reluctance to treat patients with CF with oral antibiotics due to the risk of selecting out multi-drug resistant pathogens such as S. maltophilia. However, this study demonstrated that antibiotic therapy may actually be protective against S. maltophilia by slowing lung function decline. ...
TY - JOUR. T1 - The sul1 gene in Stenotrophomonas maltophilia with high-level resistance to trimethoprim/sulfamethoxazole. AU - Chung, Hae Sun. AU - Kim, Kyeongmi. AU - Hong, Sang Sook. AU - Hong, Seong Geun. AU - Lee, Kyungwon. AU - Chong, Yunsop. N1 - Publisher Copyright: © The Korean Society for Laboratory Medicine.. PY - 2015. Y1 - 2015. N2 - Emerging resistance to trimethoprim/sulfamethoxazole (SXT) poses a serious threat to the treatment of Stenotrophomonas maltophilia infections. We determined the prevalence and molecular characteristics of acquired SXT resistance in recent clinical S. maltophilia isolates obtained from Korea. A total of 252 clinical isolates of S. maltophilia were collected from 10 university hospitals in Korea between 2009 and 2010. Antimicrobial susceptibility was determined by using the CLSI agar dilution method. The sul1, sul2, and sul3 genes, integrons, insertion sequence common region (ISCR) elements, and dfrA genes were detected using PCR. The presence of the ...
Stenotrophomonas maltophilia can present as bacteremia, respiratory tract infection, urinary tract infection, soft tissue and wound infections, bone
The expression of the Stenotrophomonas maltophilia multidrug resistance (MDR) pump SmeDEF is transcriptionally regulated by SmeT (12), a local repressor encoded by the smeT gene, which is located upstream of smeD and which is divergently transcribed. SmeT binds to the intergenic smeT-smeD region, where the promoters of smeT and smeD are located. It has previously been found that a mutation in smeT is responsible for smeDEF overproduction in MDR strain S. maltophilia D457R (1, 12). However, nothing is known about the molecular basis of smeDEF overproduction in clinical S. maltophilia isolates. For that goal, the intergenic smeT-smeD region, which contains both the smeT and smeD promoters, as well as the smeT gene, were cloned from a collection of clinical S. maltophilia strains, 33% of which were SmeDEF overproducers (2), by PCR and sequenced as described previously (12). The strains used in this work are listed in Table 1. The intergenic region and the smeT gene were highly polymorphic (Fig. 1). ...
Roseomonas gilardii is a species of Gram negative, strictly aerobic, coccobacilli-shaped, pink-pigmented bacterium. It is the type species of the genus Roseomonas. The new species was among the first Roseomonas species proposed in 1993, and is named for Gerald L. Gilardi for his many contributions to bacteriology and, specifically, for his contributions in the area of glucose-nonfermenting gram-negative rods. R. gilardii is pathogenic for humans, causing bacteremia and other infections. There are two subspecies of R. gilardii: R. g. subsp. gilardii and R. g. subsp. rosea. Rihs JD, Brenner DJ, Weaver RE, Steigerwalt AG, Hollis DG, Yu VL. Roseomonas, a new genus associated with bacteremia and other human infections. Journal of clinical microbiology. 1993 Dec 1;31(12):3275-83. Sanchez-Porro C, Gallego V, Busse HJ, Kämpfer P, Ventosa A. Transfer of Teichococcus ludipueritiae and Muricoccus roseus to the genus Roseomonas, as Roseomonas ludipueritiae comb. nov. and Roseomonas rosea comb. nov., ...
Multidrug-resistant Gram negative bacilli (MDR-GNB) are increasingly problematic in healthcare settings, primarily because of the paucity of effective antimicrobial agents that are available to treat infections with these organisms. Our MDR-GNB transmission prevention program includes hand hygiene promotion, antimicrobial stewardship, isolation precautions, environmental cleaning and disinfection, and programs to promote, monitor and sustain evidence-based best practices for Multidrug-Resistant Organism (MDRO) prevention. Tools and Resources. ...
Introduction: The present study was undertaken to determine the prevalence of non-fermenting gram negative bacilli and antibiogram of Pseudomonas aeruginosa and Acinetobacter baumannii. Materials and Methods: Conventional bacteriological methods were used for identification non fermenting gram negative bacilli and susceptibility testing was performed with the help of the Kirby-Bauer disc diffusion method. Results: A total 195 non fermenting gram negative bacilli were isolated during the study period. Pseudomonas aeruginosa and Acinetobacter baumannii isolates represent 89.23% of all isolated bacteria. Therefore, antibiotic resistance rates were performed specifically against these two bacteria. P. aerurginosa showed good sensitivity to imipenem(94.29%) followed by amikacin(86.67%) and piperacillin/tazobactum(68.57%). A. baumannii showed good sensitivity to imipenem(82.61%) followed by amikacin(76.81%). Conclusion: P. aeruginosa and A. baumannii were the most common NFGNB isolated in our study. ...
Short-term starvation of immune deficient Drosophila improves survival to gram-negative bacterial infections.. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
Abstract Objective: Recommended combination for the treatment of serious Stenotrophomonas maltophilia infections is ticarcillin-clavulanate and co-trimoxazole (SXT). However, first agent is not available in our country, and the second component may be a matter of antimicrobial resistance or intolerance. Therefore, we aimed to evaluate antimicrobial susceptibility testing results of S. maltophilia and in vitro activity […]
Dr. Yau has focused her research on the epidemiology, diagnosis and management of multi-drug resistant bacterial pahogens in cystic fibrosis (CF). Her research involve examining the epidemiology and clinical significance of Stenotrophomonas maltophilia infections in pediatric and adult CF patients, as well as determining the most effect antimicrobial combinations for the treatment of this infection in CF. She was the co-principal investigator on a multi-center randomnized controlled trial evaluating the utility of biofilm susceptibility assay in choosing more effective antibiotics to treat CF patients with chronic Pseudomonas aeruginosa pulmonary infection. She is also investigating the use of novel antimicrobial agents/modalities in the treatment of CF patients with multi-drug resistant Gram negative bacterial pulmonary infections. In addition, Dr. Yau is involved in a national multicentre study to determine the role of transmissible, clonal P. aeruginosa in the Canadian CF patient population ...
Background: The Verigene® Gram-Negative Blood Culture Test (BC-GN) detects 4 Gram-negative genera, 4 species, and 6 resistance genes within 2 hours of blood culture positivity, shortening the time from Gram stain to pathogen and resistance gene identification. The purpose of this study was to determine the impact of BC-GN testing paired with an antimicrobial stewardship intervention on antimicrobial and clinical outcomes.. Methods: This retrospective pre-post study compared patients admitted with a blood culture positive for a Gram-negative organism detectable by the BC-GN before (January 1, 2015 - July 1, 2015) and after (July 15, 2015 - January 15, 2016) this microarray test was implemented. The primary objective was to compare time from Gram stain to antimicrobial switch pre and post implementation. Secondary objectives included time from Gram stain to active treatment, in-hospital mortality, and hospital length of stay.. Results: 877 patients with Gram-negative bacteremia were included, 456 ...
An improvement resource to help health and social care economies reduce the number of Gram-negative bloodstream infections (BSIs) with an initial focus on Escherichia coli (E.coli).
This article describes the emergence of resistance and predictors of fatality for 1556 cases of healthcare-associated Gram-negative bloodstream infection in 2014 and 2015. The colistin resistance rate in Klebsiella pneumoniae ...
Trends in meticillin resistant (MRSA) and meticillin susceptible (MSSA) Staphylococcus aureus, Gram-negative bacteraemia and Clostridioides difficile infection.
Background: Gram-negative bacterial bloodstream isolates (GNBSIs) resistant to all available high-efficacy/low-toxicity (HELT) antibiotics (quinolones, penicillins, cephalosporins, carbapenems) exhibit difficult to treat resistance (DTR), which limits options for empiric and targeted therapy. The wrong empiric antibiotic may be selected even when GNBSIs remain susceptible to 1 or 2 HELT agents. Analyzing DTR trends allows assessment of the potential burden of inappropriate therapy and impact of antimicrobial resistance control interventions. Methods: Inpatient encounters (2009-2013) with ≥ 1 positive GNBSI (E. coli, Enterobacter spp., Klebsiella spp., P. aeruginosa or A. baumannii) were analyzed using PremierTM Database linked patient data from 180 US hospitals. Laboratory-reported categorical susceptibility breakpoints were used to categorize GNBSIs into respective DTR strata (non-DTR, DTR-2, DTR-1 and full DTR, denoting ,2, 2, 1 and 0 active HELT antibiotic classes, respectively). Annual ...
Follow-up blood cultures may have little utility in the management of Gram-negative bacilli bacteremia, according to data from a recent study.
402 patients enrolled. No significant difference in mortality (about 18% in both groups). No significant difference in rate of pneumonia recurrence (27%) and mean time to recurrence.. No signal with multivariate analysis, except: infections with non-lactose-fermenting Gram negative bacilli had more recurrence (i.e. same organism regrew) in the short-course group (40.6% vs 25.4%; 15.2% absolute hazard, 37.4% relative hazard).. No effects on secondary outcomes including organ dysfunction, vent-free days, ICU stay, etc, including in the subset of patients with NLFGNB infection. No difference in any outcome for MRSA pneumonias.. Significant reduction in antibiotic days, and MDR pathogens developed significantly less often in short-course group (42.1% vs 62.3%, p=.04). ...
Buy Duricef Online! Duricef is a broad-spectrum antibiotic of the cephalosporin type, effective in Gram-positive and Gram-negative bacterial infections. It is a bactericidal antibiotic.
Temocillin. Molecular model of the drug temocillin (C16.H18.N2.O7.S2), a beta-lactamase-resistant penicillin used to treat multiresistant Gram-negative bacterial infections. Atoms are represented as spheres and are colour-coded: carbon (grey), hydrogen (white), nitrogen (blue), oxygen (red) and sulphur (yellow). Illustration. - Stock Image F017/0687
Emerging evidences suggest systemic inflammation and postoperative infections lead to cancer recurrence (7-10). The mechanisms by which postoperative gram-negative bacterial infections promote cancer recurrence are poorly understood. Although LPS-induced systemic inflammation can increase cancer cell recruitment to the hepatic sinusoids (12) and liver metastasis in vivo (29), knowledge on the direct impact of LPS on the metastatic potentials of cancer cells in vivo is very limited. In this study, we have documented an increase in adhesion to endothelial cells and various ECM substrates in vitro and an enhancement in hepatic recruitment and liver metastasis in vivo by human colorectal cancer cells through LPS-induced TLR4 signaling.. Although LPS may alter proliferation and apoptosis in cancer cells (30, 31), and potentially explain the increased metastasis with LPS incubation of cancer cells, we did not show any proliferative or apoptotic changes in vitro after 4 hours of LPS treatment. This may ...
This topic has 2 study abstracts on Achillea fragrantissima indicating that it may have therapeutic value in the treatment of Polio, Candida Infection, and Gram-Negative Bacterial Infections
Background: The value of follow-up blood cultures (FUBCs) to document clearance of bacteremia due to gram-negative bacilli (GNB) has not been well-established. Although previous studies suggested the yield of FUBC for GNB bacteremia is low, it remains to be elucidated for whom FUBC may be beneficial and for whom unnecessary. The objective of the study ...
bacterial infections - MedHelps bacterial infections Center for Information, Symptoms, Resources, Treatments and Tools for bacterial infections. Find bacterial infections information, treatments for bacterial infections and bacterial infections symptoms.
bacteremia Definisi Terdapatnya bakteri dalam darah. Hampir semua prosedur kedokteran gigi dapat menimbulkan bakteremia, walaupun tidak selalu berbaha...
To Whom It May Concern Lick of death: Capnocytophaga canimorsus is an important cause of sepsis in the elderly1 describes an excellent outcome thanks to an elegant diagnostic work up and excellent treatment. However, I believe the headline Lick of death is unnecessarily fear-mongering and unwarranted (the patient, after all, did not die). Additionally, while the authors should be congratulated for educating practitioners about C. canimorsus, I feel there is scope for discussion with veterinarians to reduce further risk to this and other patients. The authors conclude that the source of the patients infection was the owners own dog, via a lick, on the grounds that Capnocytophaga canimorsus was isolated from that patient, that it is a zoonotic organism and that the patient admitted to being licked by the dog. My understanding is that this conclusion is based on findings that C canimorsus colonises the mouths of dogs and cats. This seems reasonable though for the sake of completeness and ...
We reviewed clinical and epidemiologic features of 56 human Capnocytophaga canimorsus isolates submitted during a 32-year period to California's Microbial Diseases Laboratory for identification. An increasing number of isolates identified as C. canimorsus have been submitted since 1990. Many laboratories still have difficulty correctly identifying this species.
We reviewed clinical and epidemiologic features of 56 human Capnocytophaga canimorsus isolates submitted during a 32-year period to California's Microbial Diseases Laboratory for identification. An increasing number of isolates identified as C. canimorsus have been submitted since 1990. Many laboratories still have difficulty correctly identifying this species.
Antibiotic treatment following a dog bite in an immunocompromized patient in order to prevent Capnocytophaga canimorsus infection: a case report. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
Learn Proteus Mirabilis - Gram Negative Bacilli - Microbiology - Medicine faster and easier with Picmonics unforgettable images and stories! Picmonic is research proven to increase your memory retention and test scores. Start learning today for free!
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Production of AmpC and extended spectrum beta-lactamases among urinary isolates has created a serious problem to the successful management of the urinary tract infection. The main purpose of this study was to determine the rates of the extended spectrum beta-lactamase (ESBL) production and AmpC beta-lactamase (ABL) production among urinary isolates. Among total 564 urinary isolates, 514 (91.1%) were gram negative bacilli and 50 (8.9%) were gram positive cocci. E. coli (76.1%) was the most common bacteria isolated. Staphylococcus aureus (6.7%) was the predominant gram positive bacteria isolated. 35 (6.8%) of the 514 gram negative bacilli were ESBL producers. Similarly, 14 (2.7%) of the gram negative bacilli were ABL producers. Only one isolate was ESBL and ABL co-producer. Highest rate of susceptibility of gram negative bacteria was seen toward amikacin (97.3%) followed by imipenem (94.4%). Similarly, highest rate of susceptibility among gram positive cocci was seen toward vancomycin (100%) followed by
TY - JOUR. T1 - Proteomic analysis of 17β-estradiol degradation by Stenotrophomonas maltophilia. AU - Li, Zhongtian. AU - Nandakumar, Renu. AU - Madayiputhiya, Nandakumar. AU - Li, Xu. PY - 2012/6/5. Y1 - 2012/6/5. N2 - Microbial degradation plays a critical role in determining the environmental fate of steroid hormones, such as 17β-estradiol (E2). The molecular mechanisms governing the microbial transformation of E2 and its primary degradation intermediate, estrone (E1), are largely unknown. The objective of this study was to identify metabolism pathways that might be involved in microbial estrogen degradation. To achieve the objective, Stenotrophomonas maltophilia strain ZL1 was used as a model estrogen degrading bacterium and its protein expression level during E2/E1 degradation was studied using quantitative proteomics. During an E2 degradation experiment, strain ZL1 first converted E2 to E1 stoichiometrically. At 16 h E1 reached its peak concentration, and microbial growth started. At the ...
Stenotrophomonas maltophilia is a human opportunist pathogen, with an environmental origin. Members of the species are common inhabitants of water and soils, including rhizosphere. S. maltophilia can be a plant endosymbiont and is found in animals and washed foods. This Gram-negative bacterium has intrinsic resistance to various classes of antimicrobials. Within the S. maltophilia genome, genes encoding antibiotic inactivating enzymes, multidrug efflux pumps and a quinolone resistance gene contribute to its reduced antibiotic susceptibility. Although a low virulence pathogen, S. maltophilia can cause various infections in susceptible patients. In addition, S. maltophilia isolates present important biotechnological properties, which can be carefully taken into consideration given the pathogenic potential of this microorganism. This research topic examines S. maltophilia from different perspectives, and it includes 11 articles: 1 commentary, 7 primary research articles, and 3 review articles.In the first
Stenotrophomonas maltophilia 菌血症で悩むStenotrophomonas maltophilia なお、SM菌血症と略すらしいです。感染症学雑誌 第87巻 第 5 号http://journal.kansensho.or.jp/Disp?pdf=0870050596.pdf先日、私も久々に…
Emergence of Stenotrophomonas maltophilia as a nosocomial pathogen is becoming increasingly apparent. Pleiotropic resistance characterizes S. maltophilia. Furthermore, a slow growth rate and an increased mutation rate generate discordance between in vitro susceptibility testing and clinical outcome. Despite original susceptibility, drug-resistant strains of S. maltophilia are often recovered from patients receiving beta-lactams, quinolones, or aminoglycosides. Given the disparity among various in vitro susceptibility methods, this study incorporated a unique pharmacodynamic model to more accurately characterize the bacterial time-kill curves and mutation rates of four clinical isolates of S. maltophilia following exposure to simulated multidose regimens of ceftazidime, ciprofloxacin, gentamicin, and ticarcillin-clavulanate. Time-kill data demonstrated regrowth of S. maltophilia with all four agents. With the exception of ticarcillin-clavulanate, viable bacterial counts at the end of 24 h ...
Stenotrophomonas maltophilia is an environmental bacterium found in the soil, associated with plants and animals, and in aquatic environments. It is also an opportunistic pathogen now causing an increasing number of nosocomial infections. The treatment of S. maltophilia is quite difficult given its intrinsic resistance to a number of antibiotics, and because it is able to acquire new resistances via horizontal gene transfer and mutations. Certainly, strains resistant to quinolones, cotrimoxale and/or cephalosporins-antibiotics commonly used to treat S. maltophilia infections-have emerged. The increasing number of available S. maltophilia genomes has allowed the identification and annotation of a large number of antimicrobial resistance genes. Most encode inactivating enzymes and efflux pumps, but information on their role in intrinsic and acquired resistance is limited. Non-typical antibiotic resistance mechanisms that also form part of the intrinsic resistome have been identified via mutant library
This large outbreak (N = 12) of A. xylosoxidans bloodstream infections was caused by 1 strain, which was also isolated from CVC biofilm. Symptoms of bloodstream infection probably occurred when flushes detached bacteria from the CVC biofilm. The prospective study found that 3 (10%) of 29 patients had A. xylosoxidans-positive blood cultures. Our case-control and prospective studies support the association of A. xylosoxidans bloodstream infection and CVCs, and our molecular biologic studies confirm A. xylosoxidans biofilm of the same outbreak strain on a CVC. A. xylosoxidans outbreaks reported to date have been associated with healthcare and contamination of hospital products (1,2,5,12-14), but none occurred in an outpatient setting.. The cause of this outbreak most likely was the use of contaminated multidose vials of heparin or saline flushes, leading to the formation of an A. xylosoxidans biofilm on CVCs. Case-patient 1 had been hospitalized from late October through early November at Hospital ...
Stenotrophomonas maltophilia is a pathogen that causes infections mainly in immunocompromised patients. Despite increased S. maltophilia isolation from respiratory specimens of patients with cystic fibrosis (CF), the real contribution of the microorganism to CF pathogenesis still needs to be clarified. The aim of the present study was to evaluate the pathogenic role of S. maltophilia in CF patients by using a model of acute respiratory infection in DBA/2 mice following a single exposure to aerosolized bacteria. The pulmonary bacterial load was stable until day 3 and then decreased significantly from day 3 through day 14, when the bacterial load became undetectable in all infected mice. Infection disseminated in most mice, although at a very low level. Severe effects (swollen lungs, large atelectasis, pleural adhesion, and hemorrhages) of lung pathology were observed on days 3, 7, and 14. The clearance of S. maltophilia observed in DBA/2 mouse lungs was clearly associated with an early and ...
INTRODUCTION Stenotrophomonas maltophilia is an important cause of hospital acquired infection particularly among severely debilitated and immunosuppressed patients. CASE PRESENTATION We report a case of S. maltophilia meningitis in a preterm baby boy after a neurosurgical procedure, successfully treated with trimethoprim-sulfamethoxazole and ciprofloxacin. CONCLUSION This organism should be considered as a potential cause of meningitis and trimethoprim-sulfamethoxazole and ciprofloxacin are a combination that is successful and safe for treating preterm infants.
There were 1632 admissions with 45 nosocomial Gram-negative bacteremias in 44 patients. Infection rates of 28.2/1000 admissions and 12.1/10 000 patient-days remained stable over 5 years. The mean patient age was 55.3 years (range 17-86 years); 27.3% of patients were female, and 72.8% were male. The majority (95.6%) of bloodstream infections were monomicrobial, with only one episode of polymicrobial bacteremia. Common admitting diagnoses included respiratory failure, solid organ transplant, post-surgery, and multi-trauma. Seven bacterial species were identified; Pseudomonas aeruginosa and Enterobacter spp. were most common. Sources of bacteremia included pneumonia (48.9%), followed by central venous catheterization (22.2%). The mean time from admission to hospital to development of bacteremia was 32.9 days (95% confidence interval [CI] 0-100.9), and time from admission to the ICU was slightly less at 26.0 days (95% CI 0-90.1). Antimicrobial susceptibilities were highest for imipenem, gentamicin, ...
Aeromonas hydrophila. Coloured transmission electron micrograph of thin-sectioned cells of Aeromonas hydrophila bacteria. It is a Gram- negative, rod-shaped bacillus. Aeromonas hydrophila is associated with septicaemia, pneumonia and gastroenteritis in humans. In particular it causes opportunistic infections in patients with lowered immunity, such as in cancer and liver disease. Aeromonas hydrophila has been isolated from urine, sputum, faeces and bile. It is resistant to many types of antibiotic including penicillin, but can be controlled with tetracyclins and gentamicin. Magnification: x28,800 at 6x4.5cm size. - Stock Image B220/0809
目次 中文摘要 i 英文摘要 ii 目次 iii 表目次 vii 圖目次 viii 縮寫字對照表 x 前言 1 一、 Stenotrophomonas maltophilia 1 二、 噬菌體療法 2 三、 原核生物的病毒-噬菌體 2 四、 T4-like噬菌體 3 五、 噬菌體T4複製機制 4 六、 研究動機 4 材料方法 6 一、 實驗材料 6 (一)菌種、噬菌體及質體 6 (二)藥品 6 (三)引子(primer) 6 (四)抗生素 6 (五)試劑與緩衝溶液 6 1. 抽取質體DNA試劑 6 2. 噬菌體保存液 6 3. 一般DNA電泳試劑 7 4. 脈衝式電泳(pulsed-field gel electrophoresis)試劑 7 5. RNA之抽取純化試劑 7 6. SDS-PAGE電泳試劑 7 7. 二維電泳(2D-electrohoresis)試劑 8 8. 銀染試劑 8 9. 蛋白質N端定序分析樣品準備之試劑 9 二、 實驗方法 9 (一)細菌培養與保存 9 (二)噬菌體篩選 9 (三)噬菌體培養 9 1. 小量噬菌體培養 9 2. 大量噬菌體培養 10 (四)噬菌體純化 10 ...
This report provides an all-inclusive environment of the analysis for the Hospital-Treated Gram-Negative Infections Market. The market estimates provided in the report are the result of in-depth secondary research, primary interviews and in-house expert reviews. These market estimates have been considered by studying the impact of various social, political and economic factors along with the current market dynamics affecting the Hospital-Treated Gram-Negative Infections Market growth. Along with the market overview, which comprises of the market dynamics the chapter includes a Porters Five Forces analysis which explains the five forces: namely buyers bargaining power, suppliers bargaining power, threat of new entrants, threat of substitutes, and degree of competition in the Hospital-Treated Gram-Negative Infections Market. It explains the various participants, such as system integrators, intermediaries and end-users within the ecosystem of the market. The report also focuses on the competitive ...
Rice, L.B. (2007) Emerging issues in the management of infections caused by multidrug-resistant gram-negative bacteria. Cleveland Clinic Journal of Medicine, 74, S12-S20.
Pulmonary arterial hypertension (PAH) is a life-threatening disorder characterized by elevated pulmonary artery pressure and pulmonary vascular resistance. Continuous infusion of a prostanoid, which acts as a vasodilator and anti-proliferative agent, is indicated in the treatment of patients with severe PAH. Two prostanoids are approved for intravenous (IV) use in the United States: epoprostenol (epoprostenol sodium [brand name Flolan®], Gilead, Foster City, California) and treprostinil (treprostinil sodium [brand name Remodulin®], United Therapeutics, Silver Spring, Maryland) (1). These drugs are administered to PAH patients at hundreds of treatment centers in the United States. In September 2006, CDC received a report from a PAH specialist of a suspected increase in the number of gram-negative bloodstream infections (BSIs) among PAH patients treated with IV treprostinil. CDC conducted a retrospective investigation with the assistance of several state health departments and the cooperation of ...
Bloodstream infections (BSI) are associated with high morbidity and mortality. This scenario worsens with the emergence of drug-resistant pathogens, resulting in infections which are difficult to treat or even untreatable with conventional antimicrobials. The aim of this study is to describe the epidemiological aspects of BSI caused by multiresistant gram-negative bacilli (MDR-GNB). We conducted a laboratory-based surveillance for gram-negative bacteremia over a 1-year period. The bacterial isolates were identified by MALDI-TOF/MS and the antimicrobial susceptibility testing was performed by VITEK®2. Resistance genes were identified through PCR assays. Of the 143 patients, 28.7% had infections caused by MDR-GNB. The risk factors for MDR bacteremia were male sex, age ≥ 60, previous antimicrobial use, liver disease and bacteremia caused by K. pneumoniae. K. pneumoniae was the most frequently observed causative agent and had the highest resistance level. Regarding the resistance determinants, SHV, TEM,
Background: Dog bites are the most common animal bite injuries occurring in the United States. Estimated infection rates range between 15% and 20%. Polymicrobial infections are most common. Capnocytophaga canimorsus (C. canimorsus) is a Gram-negative rod strongly associated with dog bites, and is known to cause life-threatening infection in humans. A 42-year-old woman with septic shock: An unexpected source. Richard Sacks and Kim ...
We have previously shown that Arcobacter butzleri induces intestinal, extra-intestinal, and systemic immune responses in perorally infected gnotobiotic IL-10−/− mice in a strain-dependent fashion. Here, we present a comprehensive survey of small and large intestinal expression profiles of inflammatory and regulatory mediators as well as of the matrix-degrading gelatinases MMP-2 and MMP-9 following murine A. butzleri infection. Gnotobiotic IL-10−/− mice were infected with A. butzleri strains CCUG 30485 or C1 of human and chicken origin, respectively. At day 6 following A. butzleri infection, mucin-2 mRNA, an integral part of the intestinal mucus layer, was downregulated in the colon, whereas TNF and IL-23p19 mRNA were upregulated in the ileum. Furthermore, IFN-γ, IL-17A, IL-1β, and IL-22 mRNA were upregulated in both colonic and ileal ex vivo biopsies at day 6 post strain CCUG 30485 infection. These changes were accompanied by downregulated colonic MMP-9 levels, whereas both MMP-2 and ...
Ochrobactrum anthropi is a common soil alphaproteobacterium (7) that can interact with or colonize many eukaryotic organisms, leading to different outcomes, such as disease or even growth of some plants (references 4 and 13 and references therein). O. anthropi, a close relative of brucellae, has not garnered much attention (5, 8) but is becoming increasingly recognized as a potentially problematic opportunistic and nosocomial pathogen (13). A rising number of reported cases includes some potentially life-threatening infections, such as endocarditis (6, 9, 14). These reports, together with the organisms intrinsic multiresistance to antibiotics (13), could lead to a situation resembling that of an Acinetobacter sp., whose importance as a highly problematic nosocomial pathogen skyrocketed in the past few years (12). The increasing relevance of O. anthropi to human health, together with its phylogenetic proximity to the highly pathogenic brucellae (a USDA Select Agent), prompted us to determine the ...
All bacteraemic cases, from August 2006 to September 2007 were identified by reviewing all positive blood culture results from the microbiology department of our hospital. One thousand three hundred and sixty six cases were detected in 1336 patients. The rate of true bacteremia which was 13.1 and 10.7% of cultures were contaminated. Of the 1366 episodes of bloodstream infection, 55.3% were community-acquired and 44.7% were health-care associated. Gram-positive bacteria prevailed (58.5%), followed by gram negative bacilli (38.5%). Polymicrobial bacteremia was detected in 2.2% of cases. Coagulase-negative staphylococci (CoNS) were the leading cause (550/1366 = 40.3%), whilst enterococci,Staphylococcus aureus and Streptococci represented 8, 6.4 and 3.8% respectively. Pseudomonas aeruginosa was the commonest gram-negative isolate (155/1366 = 11.3%), followed by Escherichia coli (8.2%) and Acinetobactersp. (7.3%). Fungi were isolated in
Lipopolysaccharide (LPS) biogenesis in Gram-negative microorganisms involves its biosynthesis in the cytoplasm and subsequent transportation across 3 cellular compartments towards the cell surface area. us to differentiate inhibitors Varenicline IC50 focusing on early and Varenicline IC50 past due phases of LPS biogenesis. The finding of fresh antibiotics to take care of Gram-negative infections is definitely a significant unmet clinical want. It is more challenging to take care of Gram-negative attacks than to take care of Gram-positive infections due to differences in mobile physiology. The cell envelope of Gram-negative bacterias consists of internal and external membranes having a slim coating of cell wall structure in between both membranes. The external membrane can be an asymmetric bilayer. The external leaflet comprises of lipopolysaccharide Varenicline IC50 (LPS), as the internal leaflet comprises of phospholipids (Number 1a).1,2 The assembly of LPS within the cell surface area forms a ...
Aeromonas hydrophila ATCC ® 7966D-5™ Designation: Genomic DNA from Aeromonas hydrophila TypeStrain=True Application: Water testing
Akbar, Sirwan, Rout, Simon and Humphreys, Paul (2015) Draft Genome Sequence of the Biofilm-Forming Stenotrophomonas maltophilia Strain 53. Genome Announcements, 3 (2). e00312-15. ISSN 2169-8287 Alhudhud, M, Humphreys, Paul and Laws, Andrew P. (2014) Development of A Growth Medium Suitable for Exopolysaccharide Production and Structural Characterisation by Bifidobacteria animalis ssp. lactis. Journal of Microbiological Methods, 100. pp. 93-98. ISSN 0167-7012 Bogdarina, Irina, Murphy, Helena C., Burns, Shamus P. and Clark, Adrian J.L. (2004) Investigation of the role of epigenetic modification of the rat glucokinase gene in fetal programming. Life Science, 74 (11). pp. 1407-1415. ISSN 0024-3205 Burns, Shamus P., Murphy, Helena C., Iles, Richard A. and Cohen, Robert D. (2001) Lactate supply as a determinant of the distribution of intracellular pH within the hepatic lobule. Biochemical Journal, 3. pp. 569-571. ISSN 0264-6021 Charles, C.J., Rout, S.P., Garratt, E.J., Patel, K., Laws, A.P. and ...
Certain strains of Aeromonas hydrophila are oxidase negative when grown on gram-negative selective and differential media. Of 100 strains of A. hydrophila examined, 8 were found to possess this characteristic. Information is provided on how to detect these common variants of A. hydrophila.
1N4O: Role of the disulfide bridge Cys69-Cys238 in class A b-lactamases : a structural and biochemical investigation on the b-lactamase L2 from Stenotrophomonas maltophilia
β -lactamase enzymes inactivate β -lactam antibiotics and render them ineffective for the treatment of clinically-important Gram-negative bacterial infections. Now, two new kits from Streck Inc., available in the UK from
Aeromonas hydrophila ATCC ® 7966™ Designation: TypeStrain=True Application: Quality control strain Reference material Testing Water testing
General Information: Soil bacterium that can cause opportunistic infections. Ochrobactrum anthropi is an opportunistic human pathogen usually causing infection in association with indwelling medical devices, such as catheters and drainage tubes. This organism and related species have also been isolated from soil, activated sludge, and plants. Ochrobactrum anthropi is a Gram-negative, anaerobic, motile bacterium. A common soil bacteria, it was originally considered as an opportunistic pathogen, causing infections in immunocompromised patients, patients with indwelling catheters or peritoneal dialysis but it is now emerging as a more and more important nosocomial pathogen. The first case of human infection was described in 1980. It has been isolated from blood, the urogenital tract, respiratory tract and eyes, and it can be part of the normal intestinal flora. It is resistant to many antibiotics, especially the beta-lactams. ...
1EI5: Crystal structure of a D-aminopeptidase from Ochrobactrum anthropi, a new member of the penicillin-recognizing enzyme family.
SWISS-MODEL Repository entry for A0KNI2 (ACSA_AERHH), Acetyl-coenzyme A synthetase. Aeromonas hydrophila subsp hydrophila (strain ATCC 7966 / DSM 30187 / JCM1027 / KCTC 2358 / NCIMB 9240)
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Infectious Disease News | BOSTON - The pipeline for new therapies to treat drug-resistant gram-negative bacilli is small, but measurable progress is being made, according to Helen Boucher, MD, who presented results on the progress of IDSAs 10 x 20 initiative. She and colleagues identified nine intravenous compounds in development to treat these resistant bacteria. Since a previous survey was conducted in
Rattanaumpawan, P., Ussavasodhi, P., Kiratisin, P. and Aswapokee, N. (2013) Epidemiology of Bacteremia Caused by Uncommon Non-Fermentative Gram-Negative Bacteria. BMC Infectious Diseases, 13, 167-175.
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There are many different types of bacterial infection treatments, which vary depending on the type of bacteria that is causing...