Here, we report that kidney-pancreas and kidney-islet transplants, when successful, prevent the worsening of graft survival and vascular function of the kidney graft that may occur in ESRD diabetic patients receiving kidney transplants. Noninvasive assessments of graft vascular function using RI and UAE evaluations showed that the KP and KI-s groups had better cumulative kidney graft survival at 2, 4, and 6 years than did the KD group. Expression of NOS in the kidney graft correlated well with the functional data, showing an early impairment of NO pathways in the glomerular vessels of KD-transplanted patients. Only the KP transplant group experienced a sustained compensatory hypertrophy of the kidney graft.. The transplanted kidney in ESRD type 1 diabetic patients is exposed to the deleterious effects of both diabetes and immunosuppression (34). Initially, diabetic nephropathy encompasses both functional changes (i.e., reduced renal vasodilatory reserve and reduced ability to respond to ...
Purpose: Several risk models have been developed to predict outcome after liver transplantation (LT) in the last decade. This study analyzes the ability of these risk models to predict patient, overall graft and death-censored graft survival at short- and long-term follow-up after transplantation.. *Methods: Data included information from the SRTR database on LTs from deceased donors performed in adults (>=18 years old) from January 1st, 2005 until December 31st, 2015. For all LTs the BAR-score, DRI, ET-DRI, DRM, sRRI, SOFT and D-MELD scores were calculated. Model performance was evaluated by the discriminative capacity and area under the ROC-curve (c-statistic) for patient survival, overall graft survival and death-censored graft survival. High-risk transplantations were defined as scores above 80th percentile according to the respective risk models.. *Results: In the study period, 62,294 LTs were included. Patient survival at 3 months was best predicted by the SOFT (c-statistic: 0.68) and BAR ...
In kidney transplantation, dynamic predictions of graft survival may be obtained from joint modelling of longitudinal and survival data for which a common assumption is that random-effects and error terms in the longitudinal sub-model are Gaussian. However, this assumption may be too restrictive, e.g. in the presence of outliers, and more flexible distributions would be required. In this study, we relax the Gaussian assumption by defining a robust joint modelling framework witht-distributed random-effects and error terms to obtain dynamic predictions of graft survival for kidney transplant patients. We take a Bayesian paradigm for inference and dynamic predictions and sample from the joint posterior densities. While previous research reported improved performances of robust joint models compared to the Gaussian version in terms of parameter estimation, dynamic prediction accuracy obtained from such approach has not been yet evaluated. Our results based on a training sample from the French DIVAT ...
Data & statistics on The actuarial graft and patient survival rate: The actuarial graft and patient survival rate., Actuarial Patient and Graft Survival Rates at Hopital Necker-Enfants Malades, Actuarial survival of patients grafted within 2 years of diagnosis or later: the difference is highly significant (P=0.0004). The diagnosis-transplantation (Dx-Tx) interval is not known for four patients....
Journal of Immunology Research is a peer-reviewed, Open Access journal that provides a platform for scientists and clinicians working in different areas of immunology and therapy. The journal publishes research articles, review articles, as well as clinical studies related to classical immunology, molecular immunology, clinical immunology, cancer immunology, transplantation immunology, immune pathology, immunodeficiency, autoimmune diseases, immune disorders, and immunotherapy.
TY - JOUR. T1 - A paired kidney analysis on the impact of pre-transplant anti-HLA antibodies on graft survival. AU - Michielsen, Laura A.. AU - Wisse, Bram W.. AU - Kamburova, Elena G.. AU - Verhaar, Marianne C.. AU - Joosten, Irma. AU - Allebes, Wil A.. AU - van der Meer, Arnold. AU - Hilbrands, Luuk B.. AU - Baas, Marije C.. AU - Spierings, Eric. AU - Hack, Cornelis E.. AU - van Reekum, Franka E.. AU - Bots, Michiel L.. AU - Drop, Adriaan C. A. D.. AU - Plaisier, Loes. AU - Seelen, Marc A. J.. AU - Sanders, Jan-Stephan F.. AU - Hepkema, Bouke G.. AU - Lambeck, Annechien J.. AU - Bungener, Laura B.. AU - Roozendaal, Caroline. AU - Tilanus, Marcel G. J.. AU - Voorter, Christien E.. AU - Wieten, Lotte. AU - van Duijnhoven, Elizabeth M.. AU - Gelens, Marielle. AU - Christiaans, Maarten H. L.. AU - van Ittersum, Frans J.. AU - Nurmohamed, Shaikh A.. AU - Lardy, Neubury M.. AU - Swelsen, Wendy. AU - van der Pant, Karlijn A.. AU - van der Weerd, Neelke C.. AU - ten Berge, Ineke J. M.. AU - Bemelman, ...
Immediately after renal transplantation, patients experience rapid and significant improvement of their clinical conditions and undergo considerable systemic and cellular modifications. However, some patients present a slow recovery of the renal function commonly defined as delayed graft function (DGF). Although clinically well characterized, the molecular mechanisms underlying this condition are not totally defined, thus, we are currently missing specific clinical markers to predict and to make early diagnosis of this event. We investigated, using a pathway analysis approach, the transcriptomic profile of peripheral blood mononuclear cells (PBMC) from renal transplant recipients with DGF and with early graft function (EGF), before (T0) and 24 hours (T24) after transplantation. Bioinformatics/statistical analysis showed that 15 pathways (8 up-regulated and 7 down-regulated) and 11 pathways (5 up-regulated and 6 down-regulated) were able to identify DGF patients at T0 and T24, respectively. Interestingly
Persistence of post-operative color Doppler abnormalities is linked to reduced graft survival in pediatric patients after liver ...
VELASQUEZ, Sonia Y.; GARCIA, Luis F. y ALVAREZ, Cristiam M.. Regulatory T cells and their influence in kidney allograft survival. Medicina (B. Aires) [online]. 2007, vol.67, n.5, pp.491-501. ISSN 0025-7680.. The immune response elicited by an allogenic transplant usually leads to an effector response resulting in allograft rejection; however, some individuals maintain a long-term functioning transplant without signs of rejection (operational tolerance) even in the absence of immunosuppression. It has been suggested that the same mechanisms are responsible for tolerance to self-antigens and alloantigens. One of such mechanisms is immune regulation and several cell subsets with regulatory properties have been identified. Among them, the best characterized cell populations are the regulatory T cells (Treg). Although Treg in mice are CD4+CD25+, in humans the Treg phenotype is restricted to CD4 T cells with high expression of CD25 (CD25high) and Foxp3. Phenotypic and functional analysis of ...
A recent study investigated whether the limited supply of kidneys for transplant could be alleviated by using more kidneys from deceased donors who had ...
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We can assume that the cellular content and proportions of different cell types in the HSCT graft are important in safe and efficient HSCT, as is also the spectrum of cytokines the cells secrete. However, there are no systematic studies on the immunological content of clinical HSCT grafts. In current HSCT protocols, the cell and cytokine profile of the graft is not characterized.. In the project we systematically characterise the immune cell profiles of clinical HSCT grafts and study their associations with the outcome of HSCT. From all grafts we estimate the numbers of the following cell populations ...
Dr. KORAY ERDOGAN - ASMED CLINIC - 5004 grafts - MANUAL FUE PATIENTS AGE: 40 NW: 5 Total transplanted area: 130 cm2 Total donor capacity: 8600 grafts
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New polymeric adsorbents prepared by radiation-induced graft polymerization are superior to conventional adsorbents in terms of resolution in elution
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TY - JOUR. T1 - Early renal function recovery and long-term graft survival in kidney transplantation. AU - Wan, Susan S.. AU - Cantarovich, Marcelo. AU - Mucsi, I.. AU - Baran, Dana. AU - Paraskevas, Steven. AU - Tchervenkov, Jean. PY - 2016/5/1. Y1 - 2016/5/1. N2 - Following kidney transplantation (KTx), renal function improves gradually until a baseline eGFR is achieved. Whether or not a recipient achieves the best-predicted eGFR after KTx may have important implications for immediate patient management, as well as for long-term graft survival. The aim of this cohort study was to calculate the renal function recovery (RFR) based on recipient and donor eGFR and to evaluate the association between RFR and long-term death-censored graft failure (DCGF). We studied 790 KTx recipients between January 1990 and August 2014. The last donor SCr prior to organ procurement was used to estimate donor GFR. Recipient eGFR was calculated using the average of the best three SCr values observed during the first ...
The ultimate goal of clinical transplantation is for the recipients to achieve long-term survival, with continuing graft function, that is equivalent to that of the age-matched general population. We studied subsequent outcome in kidney transplant recipients with 10 years of graft function. In all, 2202 kidney transplant recipients survived with graft function ,10 years. For 10-year survivors, the actuarial 25-year patient survival rate for primary transplant living donor (LD) recipients was 57%; graft survival, 43%. For primary transplant deceased donor (DD) recipients, the actuarial 25-year patient survival rate was 39%; graft survival, 27%. The two major causes of late graft loss were death (with graft function) and chronic allograft nephropathy (tubular atrophy and interstitial fibrosis). The two major causes of death with function were cardiovascular disease (CVD) and malignancy. For nondiabetic recipients, the mean age at death with function from CVD was 54 ± 13 years; for diabetic ...
TY - JOUR. T1 - Kidney allograft survival outcomes in combined intestinal-kidney transplant. T2 - An analysis of the UNOS/OPTN database 2000-2014. AU - Moinuddin, Irfan. AU - Yaqub, Muhammad Sohail. AU - Taber, Tim. AU - Mujtaba, Muhammad. AU - Sharfuddin, Asif. PY - 2018/1/1. Y1 - 2018/1/1. N2 - Background and objectives: Intestinal transplants carry a high morbidity/mortality. Kidney allograft outcomes after combined intestinal (IT) with kidney transplant (CIKT) remain largely uninvestigated. Materials and methods: The UNOS STAR database was queried to identify all such combined organ transplants from 2000 to 2015. Results: Out of a total 2215 (51.4% peds vs 48.6% adults) intestinal transplants, 111 (5.0%) CIKT were identified (32.4% peds vs 67.6% adults). Over the study period of CIKT, a total of 45.9% of these cases died with a functioning kidney graft. DGF rate was 9.0%. The 1-year reported kidney acute rejection rate was 6.3%. For the entire CIKT population over the entire study era, the ...
Association between Early Resistive Index Measurement and Early Graft Function and Long-Term Graft Survival after Kidney Transplantation: an Evidence-based Clinical Review
Multiple studies have documented racial differences in graft survival in kidney transplant recipients. Although several studies in adult kidney transplant recipients have evaluated risk factors that might predispose to these differences, studies in pediatric patients are lacking. This study retrospectively analyzed data from the North American Pediatric Renal Trials and Collaborative Studies (NAPRTCS) to identify racial differences in kidney transplant outcomes and evaluate factors that might contribute to those differences. The study was restricted to the first NAPRTCS registry-reported kidney transplant for pediatric patients (age | or =21 yr) whose race was reported as either black or white. Univariate graft survival analyses were performed using the log rank statistic. Relative hazard rates for the effect of race on graft failure were determined using proportional hazards models. Multivariate analyses were restricted to patients with |30 d of graft survival and were adjusted for initial diagnosis,
Background. Chronic allograft nephropathy (CAN) is the leading cause of late allograft failure, with only limited treatment options. Blockade of the renin-angiotensin system (RAS) decreases progression in diabetic and non-diabetic renal disease, but the effect on CAN is as yet unclear. Therefore, we have studied retrospectively the effect of RAS blockade on renal survival in patients with biopsy-proven CAN.. Methods. The medical records of 72 patients with biopsy-proven CAN were evaluated with regard to time course of graft function, proteinuria, blood pressure, and antihypertensive and immunosuppressive treatment. Coxs proportional hazards model was used for analysing renal graft survival after the index biopsy.. Results. On univariate analysis, histological determinants influencing renal survival were the chronic interstitial and chronic tubular score, and clinical parameters were the serum creatinine level at the time of the biopsy, the relative change in serum creatinine level between 12 ...
BACKGROUND: The clinical and immunological relevance of a positive B-cell flow-cytometry (B-FCXM) crossmatch in renal transplantation is still controversial. METHODS: We retrospectively analysed 145 consecutive cadaveric renal transplantations performed from May 1991 to September 1995 in our institution. All grafts were transplanted following a negative IgG T-cell complement-dependent cytotoxicity crossmatch (T-CDCXM). Concomitantly to CDCXM, B-cell and T-cell FCXM were performed and results were expressed as a mean fluorescence index (FI). Two groups were compared: 116 recipients grafted with a negative B-FCXM vs a group of 19 patients grafted with a positive B-FCXM. RESULTS: The two groups were similar for length of cold ischaemia, donor and recipients age and degree of HLA mismatching. The proportion of patients with pre-transplant anti-HLA class I antibodies or a retransplantation was significantly increased in the positive B-FCXM group vs the negative B-FCXM group. Recipient survival at 48 ...
TY - JOUR. T1 - Allogeneic uterus transplantation in baboons. T2 - Surgical technique and challenges to long-term graft survival. AU - Tryphonopoulos, Panagiotis. AU - Tzakis, Andreas G.. AU - Tekin, Akin. AU - Johannesson, Liza. AU - Rivas, Krishna. AU - Morales, Pablo R.. AU - Wagner, Joseph. AU - Mölne, Johan. AU - Enskog, Anders. AU - Diaz-Garcia, Cesar. AU - Dahm-Kähler, Pernilla. AU - Berho, Mariana. AU - Zimberg, Stephen. AU - Falcone, Tommaso. AU - Ruiz, Philip. AU - Olausson, Michael. AU - Brännström, Mats. PY - 2014/1/1. Y1 - 2014/1/1. UR - http://www.scopus.com/inward/record.url?scp=84907397669&partnerID=8YFLogxK. UR - http://www.scopus.com/inward/citedby.url?scp=84907397669&partnerID=8YFLogxK. U2 - 10.1097/TP.0000000000000322. DO - 10.1097/TP.0000000000000322. M3 - Letter. C2 - 25171537. AN - SCOPUS:84907397669. VL - 98. SP - e51-e56. JO - Transplantation. JF - Transplantation. SN - 0041-1337. IS - 5. ER - ...
Antigen-presenting cells (APCs) play an important role in transplant rejection and tolerance. In high-risk corneal transplantation, where the graft bed is inflamed and vascularized, immature APCs in the donor corneal stroma quickly mature and migrate to lymphoid tissues to sensitize host T cells. In this study, using a mouse model of corneal transplantation, we investigated whether enrichment of tolerogenic APCs (tolAPCs) in donor corneas can enhance graft survival in corneal allograft recipients with inflamed graft beds. Treatment of donor corneas with interleukin-10 (IL-10) and transforming growth factor-β1 (TGFβ1) altered the phenotype and function of tissue-residing APCs. Transplantation of these tolAPC-enriched corneas decreased frequencies of interferon gamma (IFNγ)+ effector T cells (Teffs), as well as allosensitization in the hosts, diminished graft infiltration of CD45+ and CD4+ cells, and significantly improved corneal allograft survival compared to saline-injected controls. These data
Background:Delayed graft function (DGF) is closely associated with the use of marginal donated kidneys due to deficits during transplantation and in recipients. We aimed to predict the incidence of DGF and evaluate its effect on graft survival.Methods:|/s...
TY - JOUR. T1 - Late graft loss or death in pediatric liver transplantation. T2 - an analysis of the SPLIT database. AU - Soltys, K. A.. AU - Mazariegos, G. V.. AU - Squires, R. H.. AU - Sindhi, R. K.. AU - Anand, R.. AU - Dunn, Stephen. AU - Manendez, Jerome. AU - Flynn, Louise. AU - Jonas, Maureen. AU - Krawczuk, Laura. AU - Christoff, Marielle. AU - Kane, Robert. AU - Solomon, Harvey. AU - Phillips, Erin. AU - Ferrer, Laurie. AU - Heffron, Thomas. AU - DePaolo, Jill. AU - Pillen, Todd. AU - Davis, Laurel. AU - Bucuvalas, John. AU - Ryckman, Fred. AU - Hawkins, Andre. AU - Arya, Gajra. AU - Narkewicz, Michael R.. AU - Sokol, Ronald J.. AU - Karrer, Frederick. AU - Mark, Cara. AU - Orban-Eller, Kathy. AU - Humar, Abhi. AU - Durand, Brenda. AU - Studenski, Leslie. AU - Rand, Elizabeth. AU - Anderer, Kathleen. AU - Mazariegos, George. AU - Chien, Nydia. AU - Seward, Lynn. AU - Atkison, Paul. AU - Roden, Jay. AU - Mittal, Naveen. AU - Cutright, Lisa. AU - Telega, Grzegorz. AU - Lerret, ...
The impact of FSGS on graft survival in children is greatest in LD transplants, resulting in loss of expected LD graft survival advantage. The rationale for LD grafts in children with FSGS should be based on factors other than better outcomes typically associated with LD transplantation.
Introduction The ability to preserve organs prior to transplant is essential to the organ allocation process. Objective The purpose of this study is to describe the functional relationship between cold-ischemia time (CIT) and primary nonfunction (PNF), patient and graft survival in liver transplant. Methods To identify relevant articles Medline, EMBASE and the Cochrane database, including the non-English literature identified in these databases, was searched from 1966 to April 2008. Two independent reviewers screened and extracted the data. CIT was analyzed both as a continuous variable and stratified by clinically relevant intervals. Nondichotomous variables were weighted by sample size. Percent variables were weighted by the inverse of the binomial variance. Results Twenty-six studies met criteria. Functionally, PNF% = −6.678281+0.9134701*CIT Mean+0.1250879*(CIT Mean−9.89535)2−0.0067663*(CIT Mean−9.89535)3, r2 = .625, , p|.0001. Mean patient survival: 93 % (1 month), 88 % (3 months), 83 %
Abstract:. Background. Liver transplantation is the standard of care for the treatment of liver failure worldwide, yet millions of people living in sub-Saharan Africa remain without access to these services. South Africa (SA) has two liver transplant centres, one in Cape Town and the other in Johannesburg, where Wits Donald Gordon Medical Centre (WDGMC) started an adult liver transplant programme in 2004.. Objectives. To describe the outcomes of the adult liver transplant programme at WDGMC.. Methods. This was a retrospective review of all adult orthotopic liver transplants performed at WDGMC from 16 August 2004 to 30 June 2016 with a minimum follow-up of 6 months. The primary outcome was recipient and graft survival and the effect of covariates on survival. Kaplan-Meier survival analysis included all adults who underwent their first transplant for end-stage liver disease (ESLD) (N=275). Proportional hazards regression analysis using hazard ratios (HRs) was conducted to determine which ...
Background. The molecular mechanism of small-forsize graft injury remains unclear. The aim of this study is to investigate the gene expression pattern of acute phase response in relation to graft size in a rat-liver transplantation model. Methods. A rat orthotopic liver transplantation model using 30%, 50%, and whole grafts was used. The graft survival rates and liver morphology were compared among the three groups. Two transcription factors, nuclear factor (NF)-κB (p65) and early growth response (Egr-1), and their downstream genes were compared. Results. According to the graft size, the rats were grouped as follows: group 1 (n=20), 32% (24-47%); group 2 (n=10), 56% (50-65%); and group 3 (n=10), 104% (89-120%). The 7-day survival rates were 20% (P=0.039 vs. group 2, P=0.000 vs. group 3), 60%, and 100% in groups 1, 2, and 3, respectively. Dilation of hepatic sinusoids and vacuolization of hepatocytes were observed in group 1. Up-regulation of Egr-1 and endothelin (ET)-1 and over-expression of ...
A new kidney allocation system, expected to be implemented in late 2014, will characterize donors on a percent scale (0%-100%) using the kidney donor profile index (KDPI). The 20% of deceased donor kidneys with the greatest expected posttransplant longevity will be allocated first to the 20% of candidates with the best expected posttransplant survival; kidneys that are not accepted will then be offered to remaining 80% of candidates. Waiting time will start at the time of maintenance dialysis initiation (even if before listing) or at the time of listing with an estimated glomerular filtration rate of 20 mL/min/1.73 m(2) or less. Under the current system, the number of candidates on the waiting list continues to increase, as each year more candidates are added than are removed. Median waiting times for adults increased from 3 years in 2003 to more than 4.5 years in 2009. Donation rates have not increased. Short-term outcomes continue to improve; death-censored graft survival at 90 days ...
Skin allograft survival.Compared to the control group, only the combination cell therapy significantly prolonged graft survival (p = 0.028).
Modern immunosuppression regimens effectively control acute rejection and decrease graft loss in the first year after transplantation; however, these regimens do not have a durable effect on long-term graft survival owing to a combination of drug toxicities and the emergence of chronic alloimmune responses. Eliminating drugs and their toxicities while maintaining graft acceptance has been the primary aim of cellular therapies. Tregs suppress both autoimmune and alloimmune responses and are particularly effective in protecting allografts in experimental transplant models. Further, Treg-based therapies are selective, do not require harsh conditioning, and do not have a risk of graft-versus-host disease. Trial designs should consider the distinct immunological features of each transplanted organ, Treg preparations, dose, and frequency, and the ability to detect and quantify Treg effects in a given transplant environment. In this Review, we detail the ongoing clinical trials of Treg therapy in liver ...
Modern immunosuppression regimens effectively control acute rejection and decrease graft loss in the first year after transplantation; however, these regimens do not have a durable effect on long-term graft survival owing to a combination of drug toxicities and the emergence of chronic alloimmune responses. Eliminating drugs and their toxicities while maintaining graft acceptance has been the primary aim of cellular therapies. Tregs suppress both autoimmune and alloimmune responses and are particularly effective in protecting allografts in experimental transplant models. Further, Treg-based therapies are selective, do not require harsh conditioning, and do not have a risk of graft-versus-host disease. Trial designs should consider the distinct immunological features of each transplanted organ, Treg preparations, dose, and frequency, and the ability to detect and quantify Treg effects in a given transplant environment. In this Review, we detail the ongoing clinical trials of Treg therapy in liver ...
Modern immunosuppression regimens effectively control acute rejection and decrease graft loss in the first year after transplantation; however, these regimens do not have a durable effect on long-term graft survival owing to a combination of drug toxicities and the emergence of chronic alloimmune responses. Eliminating drugs and their toxicities while maintaining graft acceptance has been the primary aim of cellular therapies. Tregs suppress both autoimmune and alloimmune responses and are particularly effective in protecting allografts in experimental transplant models. Further, Treg-based therapies are selective, do not require harsh conditioning, and do not have a risk of graft-versus-host disease. Trial designs should consider the distinct immunological features of each transplanted organ, Treg preparations, dose, and frequency, and the ability to detect and quantify Treg effects in a given transplant environment. In this Review, we detail the ongoing clinical trials of Treg therapy in liver ...
Modern immunosuppression regimens effectively control acute rejection and decrease graft loss in the first year after transplantation; however, these regimens do not have a durable effect on long-term graft survival owing to a combination of drug toxicities and the emergence of chronic alloimmune responses. Eliminating drugs and their toxicities while maintaining graft acceptance has been the primary aim of cellular therapies. Tregs suppress both autoimmune and alloimmune responses and are particularly effective in protecting allografts in experimental transplant models. Further, Treg-based therapies are selective, do not require harsh conditioning, and do not have a risk of graft-versus-host disease. Trial designs should consider the distinct immunological features of each transplanted organ, Treg preparations, dose, and frequency, and the ability to detect and quantify Treg effects in a given transplant environment. In this Review, we detail the ongoing clinical trials of Treg therapy in liver ...
Modern immunosuppression regimens effectively control acute rejection and decrease graft loss in the first year after transplantation; however, these regimens do not have a durable effect on long-term graft survival owing to a combination of drug toxicities and the emergence of chronic alloimmune responses. Eliminating drugs and their toxicities while maintaining graft acceptance has been the primary aim of cellular therapies. Tregs suppress both autoimmune and alloimmune responses and are particularly effective in protecting allografts in experimental transplant models. Further, Treg-based therapies are selective, do not require harsh conditioning, and do not have a risk of graft-versus-host disease. Trial designs should consider the distinct immunological features of each transplanted organ, Treg preparations, dose, and frequency, and the ability to detect and quantify Treg effects in a given transplant environment. In this Review, we detail the ongoing clinical trials of Treg therapy in liver ...
Modern immunosuppression regimens effectively control acute rejection and decrease graft loss in the first year after transplantation; however, these regimens do not have a durable effect on long-term graft survival owing to a combination of drug toxicities and the emergence of chronic alloimmune responses. Eliminating drugs and their toxicities while maintaining graft acceptance has been the primary aim of cellular therapies. Tregs suppress both autoimmune and alloimmune responses and are particularly effective in protecting allografts in experimental transplant models. Further, Treg-based therapies are selective, do not require harsh conditioning, and do not have a risk of graft-versus-host disease. Trial designs should consider the distinct immunological features of each transplanted organ, Treg preparations, dose, and frequency, and the ability to detect and quantify Treg effects in a given transplant environment. In this Review, we detail the ongoing clinical trials of Treg therapy in liver ...
TY - JOUR. T1 - Adult liver transplantation in the USA. AU - Alqahtani, Saleh Ali. AU - Larson, Anne M.. PY - 2011. Y1 - 2011. N2 - Purpose of review: To provide the nontransplant clinician with a basic understanding of the liver transplant process. Recent findings: Since its inception, the technique of liver transplantation and patient management has evolved considerably. We present an up-to-date overview of the evaluation of the transplant recipient and the listing and timing of transplant. We conclude with a brief summary of long-term complications, which should be considered when caring for the posttransplant patient. Summary: Liver transplantation is the only definitive treatment option for patients dying of liver failure. The growing population of patients with liver disease means that more transplants will be performed. As these patients now live longer lives, it is crucial that clinicians have a basic understanding of the process and outcomes.. AB - Purpose of review: To provide the ...
Results: In untreated control groups (n=5) animals rejected skin grafts, solid organ grafts and hind limb transplants acutely by POD 14 (± 1 day), POD 9 (± 2 days), and 8 (± 1 day), respectively. The induction regimen extended skin graft survival to 32 ± 8 days (n=5). Additional donor BM augmentation lead to allograft survival of 150 days in 4 of 5 recipients. However, indefinite graft survival of ,150 days was observed in all animals receiving the induction regimen and a VCA. Mixed chimerism analysis showed engraftment of donor BM in groups receiving BM at the time of skin transplantation (6.8% ± 3.1%). In groups receiving a VCA alone or an allograft augmented with donor BM and splenocytes, donor chimerism was detected at 22.51% ± 5.96% and 30.17% ± 8.72%, respectively. Vβ-T cell receptor staining showed decreased expression of Vβ 5.1/5.1 and Vβ 8.1/8.2 in animals receiving the full induction regimen compared to controls indicating a central selection and depletion mechanism for ...
This cohort study evaluates the association of deceased donor acute kidney injury with recipient graft survival and characterizes recovery and discard practices
This research grant spans a multidisciplinary innovation experiment involving reproductive preservation, biomolecular imaging and regenerative medicine. The purpose is to explore the most frequent multiple factors to affect the survival rate of immature testicular grafts which deserves to improve the survival rate of the immature graft from lacking spermatogenesis to spermatogenesis over time in the long run. Our long term series of applied bioluminescence imaging in vivo to track the grafts under 3-D in vivo imaging system (IVIS) system to analyze the graft survival in a longitudinal real-time model that may create much faster and more convicing data on a quality and quantity basis even in the experiment to adjust the drugs delivery or able to reduce multiple error trials. In the experiment, the same animals were used for continuous observation to reduce untoward sacrified mouse model that may meet the 3R principles of substitution, reduction and sophistication. While the fate mapping of ...
Aim: To evaluate if baseline serum lipids are associated with islet graft survival in type 1 diabetes mellitus islet transplant (ITx) recipients.. Research design and methods: Baseline fasting lipid profile was collected from 44 ITx recipients. Comparisons were performed between subjects below and above the median values of each lipid fraction. Differences in outcomes were compared by Kaplan-Meier curves and Cox-regression analysis.. Results: Subjects with baseline fasting plasma triglycerides and VLDL-cholesterol above median had shorter islet graft survival (triglycerides: 39.7±6.1 vs. 61.3±6.6 months, P=0.029 and VLDL: 41.5±5.7 vs. 62.8±7.3 months, P=0.032). Total, LDL and HDL-cholesterol didnt influence islet function. Triglycerides (OR=2.97, 95%CI=1.03-8.52, P=0.044) maintained its association with graft failure after adjustments for confounders.. Conclusions: Higher baseline triglycerides are associated with earlier decline in islet graft function. Prospective clinical trials should ...
Shapiro, R and Tzakis, AG and Hakala, TR and Lopatin, WB and Stieber, AC and Starzl, TE (1989) Renal transplantation in black recipients at the University of Pittsburgh. Transplantation Proceedings, 21 (6). 3921 - 3925. ISSN 0041-1345 ...
Fingerprint Dive into the research topics of Islet allograft survival in nonhuman primates immunosuppressed with basiliximab, RAD, and FTY720. Together they form a unique fingerprint. ...
When Cetrulos team used skin from these Gal-free pigs to provide grafts covering burn-like injuries on the backs of baboons - injuries made while the animals were under anesthesia - the grafts adhered and developed a vascular system within 4 days of implantation. Signs of rejection began to appear on day 10, and rejection was complete by day 12 - a time frame similar to what is seen with deceased-donor grafts and identical to that observed when the team used skin grafts from other baboons. As with the use of second deceased-donor grafts to treat burned patients, a second pig-to-baboon graft was rapidly rejected. But if a pit-to baboon was followed by a graft using baboon skin, the second graft adhered to the wound and remained in place for around 12 days before rejection. The researchers also showed that acceptance of a second graft was similar no matter whether a pig xenograft or a baboon skin graft was used first ...
TY - JOUR. T1 - Whats the score?. AU - Nyberg, Scott L.. PY - 2004/8/1. Y1 - 2004/8/1. N2 - In summary, a quantitative approach to assessing quality of donor organs, such as a donor scoring system, is not perfect as pointed out by Sing et al. However, in all of our studies involving deceased donors we have found that consideration of multiple donor variables is more predictive of long-term graft survival than any single donor variable. The donor score is a practical tool as it provides a continuum of points to estimate likelihood of success following transplantation. In the case of a deceased donor kidney with improved donor score less than 20 points, there is over 75% likelihood of good or excellent renal function at one year post-transplant. In contrast, if the donor score is 20-29 points, the likelihood of good-to-excellent function falls to 45%, and with a donor score of 30 or greater the likelihood of good-to-excellent function falls to 27%. We do not feel that a scoring system should be ...
One month following graft injection, no significant difference was noted between M2-supplemented (105±7.0 mm3) and control graft volumes (72±22 mm3). By three months post-injection, M2-supplemented grafts remained stable while controls experienced further volume loss (103±8 mm3 vs. 39.4±15 mm3, p=0.015). Presence of M2 macrophages in the supplemented grafts was confirmed by flow cytometry. M2-supplemented grafts demonstrated a 157% increase in vascular density compared to controls (p,0.05). Induction of adipogenic C/EBPα gene expression was observed when M2 supernatants were added to SVF containing ASC ...
Joseph Phan, Mercer University College of Pharmacy The Kidney Disease: Improving Global Outcomes (KDIGO) clinical practice guideline for the care of kidney transplant recipients states that prolonged maintenance of immunosuppressive therapy after kidney transplantation has improved short-term outcomes in many cases, however this effect on long-term allograft survival is not known. The current standard-of-care combines…
Liver grafting is now established as the optimal treatment for patients with end-stage parenchymal liver disease. Twenty-three years of experience at a single center is presented. The 1-year actuarial patient survival rate for all cases transplanted in Cambridge has now risen from 10% in 1968 to 1970 to 80% in 1990 to 1991. Increasing numbers of patients are being referred for transplantation with an ever-increasing range of indications being developed. Many inborn errors of metabolism can now be cured by liver grafting. There is still, however, considerable scope for improvement in many areas of patient treatment from operative and postoperative care to long-term immunosuppressive management. Much remains to be done to minimize early sepsis- and rejection-related deaths and late immunosuppression-related morbidity.
Purpose: Our goal for this study was to determine the relative fates of endogenous Foxp3- conventional (Tconv) and Foxp3+ regulatory T cells (Tregs) specific for alloantigens as compared to non-antigen-specific T cells in the context of murine allotransplantation.. Methods: To selectively study alloreactive endogenous Tregs, donor hearts from F1 (BALB/cxC57BL/6) mice expressing the model antigen 2W were transplanted into C57BL/6 recipients, and 2W:I-Ab tetramers were used to track peripheral 2W:I-Ab-specific Tregs and Tconv cells. Long-term graft survival was induced in two groups of mice with co-stimulation blockade (CoB) comprising either anti-CD154 (D0, 7 and 14) + donor splenocytes (D0), or CTLA-4Ig (D0 and 2). Mice were sacrificed at D7 and D30, and their splenic T cells were phenotyped.. Results:Whereas the percentages or total numbers of 2W-reactive Tregs in secondary lymphoid organs were similar at d7 in naïve, acutely rejecting, and CoB-treated mice, at 30d post-transplant, recipients ...
Transplant Immunosuppression 2019: Hot Topics will focus on current options for immunosuppression (and whats in the pipeline), with particular attention to individualization of immunosuppression based on clinical and/or laboratory parameters; prevention, diagnosis and treatment of antibody-mediated rejection; improving long-term transplant outcomes; and major issues in transplant-related infectious disease, living donation, and patient-centered care.
MP preservation of DCD kidneys is superior to CS in terms of reducing DGF rate post-transplant. However, primary non-function, one year graft survival, and one year patient survival were not affected by the use of MP or CS for preservation.
How is Long-Lasting Delayed Graft Function abbreviated? LLDGF stands for Long-Lasting Delayed Graft Function. LLDGF is defined as Long-Lasting Delayed Graft Function very rarely.
To investigate the causes of gastrointestinal bleeding (GIB) and its impact on patient and graft survival after orthotopic liver transplantation (OLTx), the first 1000 consecutive OLTx using tacrolimus were studied. Our patient population consisted of 834 adults. The bleeding episodes of patients with GIB (n=74) were analyzed, and patients without GIB (n=760) were used as controls. The mean age, gender, and United Network for Organ Sharing status were similar in both groups. Endoscopy was done in 73 patients with GIB and yielded a diagnosis in 60 patients (82.2%): 39 with a single, and 21 with multiple GIB episodes. In the remaining 13 patients (17.8%), the bleeding source was not identified. Of 92 GIB episodes with endoscopic diagnoses, ulcers (n=25) were the most common cause of bleeding, followed by enteritis (n=24), portal hypertensive lesions (n=15), Roux-en-Y bleeds, and other miscellaneous events (n=28). The majority (73%) of the GIB episodes occurred during the first postoperative ...
Novel research reveals racial and socioeconomic disparities among pediatric liver transplant patients. Findings published in Liver Transplantation, a journal of the American Association for the Study of Liver Diseases and ...
Here, we will know about FAQs common questions on the Kidney transplant 1. Which is the best treatment option for chronic kidney disease patient? Renal transplant is the best treatment option for the majority of chronic kidney patients. 2. What are the two kinds of transplant? Kidney for transplant may come from a person who … Read more ...
Kidney transplant at Emory Transplant Center is a nationally recognized program, performing more than 5,000 kidney transplants to date
How is Combined Pancreas-/Kidney Transplantation abbreviated? CPKT stands for Combined Pancreas-/Kidney Transplantation. CPKT is defined as Combined Pancreas-/Kidney Transplantation somewhat frequently.
Over time, off-pump bypass surgery grafts may fail. This eMedTV resource discusses graft failure and off-pump bypass surgery, including how long grafts typically last and tips that can help delay graft failure after surgery.
This study demonstrates for the first time, to our knowledge, the induction of stable insulin-free long-term operational tolerance in diabetic nonhuman primates given a single-donor allogeneic islet transplant with multiple MHC incompatibilities. Notably, all IPIT recipients given F(Ab)2-IT plus DSG experienced prolonged graft survival without maintenance immunosuppressive therapy or exogenous insulin and several (four of seven) are currently ,1-year survivors in excellent health with documented normoglycemia and immune competence.. Sharp et al. (26) first demonstrated the potential of the STZ-induction model for diabetes studies in primates. The studies of Theriault et al. (17) and Jonasson et al. (18) meticulously characterized glycemic parameters in STZ-induced cynomolgus and rhesus macaques, respectively. Jonasson et al. (18) also detailed secondary complications in an 11-year follow up of rhesus STZ-induced type 1 diabetes. With the caveat that evidence for autoimmune involvement is ...
Judith A. Kapp, PhD Dr. Kapps research focuses on identifying mechanisms of inducing and abrogating immunological tolerance. Our long-term goal is to translate our findings into novel therapies for preventing graft rejection and augmenting tumor immunity.. A long-term goal is to use knowledge gained from tumor studies and the induction of tolerance to develop methods to prolong graft survival. We have studied two transplantation models. This first involves transplantation of retinal pigment epithelial (RPE) cells as a treatment for age-related macular degeneration (AMD), which is the leading cause of blindness in people over the age of 65 in this country. This disease ultimately results from the loss of light sensing (photoreceptor) cells. However, the loss of photoreceptor cells is preceded by loss of the underlying RPE. Replacement of dead or damaged cells with healthy retinal cells is a very promising approach to the treatment of this, and other, retinal diseases that we are investigating. ...
FTY720 was originally developed for preventing allograft rejection (Adachi et al., 1995; Chiba et al., 1999; Suzuki, 1999). It prolongs graft survival in recipient rats with liver allografts and in canine kidney recipients, partly because of lymphocyte apoptosis, especially in CD4-positive cells (Enosawa et al., 1996). Furthermore, FTY720 is known to induce apoptosis in several cell lines and primary lymphocytes in vivo and in vitro (Shinomiya et al., 1997; Matsuda et al., 1998,1999; Wang et al., 1999; Nagahara et al., 2000; Sonoda et al., 2001). Apoptosis, a programmed cell death, or the cellular suicide program, is a fundamental biological process that plays requisite roles in the development, differentiation, and maintenance of cells. Inappropriate or dysregulated apoptosis, or failure to undergo programmed cell death, has been implicated in a number of diseases and pathological conditions (Thompson, 1995).. Understanding of biochemical events in apoptosis was significantly advanced by the ...
The mediation of transplant rejection by allogeneic immune responses remains the main impediment to prolonged graft survival and to the achievement of tolerance. Adequate cell activation and differentiation is required for efficient immune responses to develop, and the balance between regulatory and effector cells serves as a key determinant of transplantation outcome. Understanding of the different signalling pathways and transcriptional patterns which characterize particular immune cell subsets led to the identification of novel targets for the improved monitoring and treatment of transplanted patients. More recently, it has emerged that the reprogramming of complex intracellular metabolic pathways also plays a crucial part in shaping immune cell function.1 As a consequence, attention has turned toward the interrogation and manipulation of the metabolic status of immune cells with a view to developing novel monitoring tools and therapeutic approaches.. Because of their lower metabolic ...