Zhang Y, Wang L, Zhou W, Wang H, Zhang J, Deng S, Li W, Li H, Mao Z, Ma D. Tissue factor pathway inhibitor-2: A novel gene involved in zebrafish central nervou
TY - JOUR. T1 - Effect of ethanol on the synthesis and secretion of hepatic secretory glycoproteins and albumin. AU - Tuma, D. J.. AU - Jennett, R. B.. AU - Sorrell, Michael Floyd. PY - 1981/1/1. Y1 - 1981/1/1. N2 - The effects of ethanol on the synthesis and secretion of serum glycoproteins and albumin, a nonglycosylated protein, were studied in rat liver slices. Serum glycoproteins and albumin were determined by immunoprecipitation from either the incubation medium or from the washed slices. When ethanol (10 mM) was present in the incubation medium, [14C]glucosamine incorporation in secretory glycoproteins was decreased. This inhibitory effect was, however, much greater in the secretory proteins released into the medium than in those retained in the liver slices. Similar inhibitions by ethanol were also observed when leucine or valine were used as a label for either total export proteins or albumin. Since ethanol impaired protein synthesis, an inhibitor of protein synthesis, cycloheximide, was ...
https://www.dualsystems.com/wp-content/uploads/sites/7/2018/07/2018-Dualsystems-Biotech-Logo-website.png 0 0 helene https://www.dualsystems.com/wp-content/uploads/sites/7/2018/07/2018-Dualsystems-Biotech-Logo-website.png helene2018-07-02 13:19:032018-08-13 16:58:18Leukocyte differentiation by histidine-rich glycoprotein/stanniocalcin-2 complex regulates murine glioma growth through modulation of anti-tumor immunity ...
Stanniocalcin-2 (STC2), the paralog of STC1, has been suggested as a novel target of oxidative stress response to protect cells from apoptosis. The expression of STC2 has been reported to be highly correlated with human cancer development. In this study, we reported that STC2 is a HIF-1 target gene and is involved in the regulation of cell proliferation. STC2 was shown to be up-regulated in different breast and ovarian cancer cells, following exposure to hypoxia. Using ovarian cancer cells (SKOV3), the underlying mechanism of HIF-1 mediated STC2 gene transactivation was characterized. Hypoxia-induced STC2 expression was found to be HIF-1α dependent and required the recruitment of p300 and HDAC7. Using STC2 promoter deletion constructs and site-directed mutagenesis, two authentic consensus HIF-1 binding sites were identified. Under hypoxic condition, the silencing of STC2 reduced while the overexpression of STC2 increased the levels of phosphorylated retinoblastoma and cyclin D in both SKOV3 and MCF7
The ability of PS to enhance the inhibition of FXa by plasma TFPI and by recombinant TFPIα produced in Escherichia coli was originally demonstrated by Hackeng et al.3 Subsequent studies have investigated the interaction of PS and TFPIα in plasma, finding that patients with PS deficiency have decreased plasma TFPIα, that immunodepletion of PS depletes plasma TFPIα as well, and that plasma TFPIα correlates with free PS, rather than with C4bp-bound PS.8 Thus, it seems that there is a physiologically relevant PS-TFPI anticoagulant system functioning in vivo, yet much remains unclear. The majority of intravascular TFPI is TFPIβ on the endothelium, which does not have the PS-binding K3 domain.24,25 In addition, the repercussions of FXa inhibition by PS-TFPIα are not apparent, as TFPIα is a poor inhibitor of thrombin production by the prothrombinase complex assembled with FXa and thrombin-activated FVa, and PS does not enhance this inhibitory function to physiologically relevant rates (Figure ...
Methods and Results-Concomitant with the angiogenic and proliferative responses, hVEGF-DΔNΔC enhanced the phosphorylation of VEGF receptor-2, Akt, and endothelial nitric oxide synthase. Gene arrays, quantitative reverse transcription-polymerase chain reaction, and Western blot revealed increases in VEGF-A, stanniocalcin-1 (STC1), and neuropilin (NRP) 2 expression by hVEGF-DΔNΔC stimulation, whereas induction with hVEGF-A165 altered the expression of STC1 and NRP1, another coreceptor for VEGFs. The effects of hVEGF-DΔNΔC were seen only under high-serum conditions, whereas for hVEGF-A165, the strongest response was observed under low-serum conditions. The hVEGF-DΔNΔC-induced upregulation of STC1 and NRP2 was also evident in vivo in mouse skeletal muscle treated with hVEGF-DΔNΔC by adenoviral gene delivery. The importance of NRP2 in hVEGF-DΔNΔC signaling was further studied with NRP2 small interfering RNA and NRP antagonist, which were able to block hVEGF-DΔNΔC-induced survival of ...
Sigma-Aldrich offers abstracts and full-text articles by [J Rollin, S Iochmann, C Bléchet, F Hubé, S Régina, S Guyétant, E Lemarié, P Reverdiau, Y Gruel].
We used domain-selective biotinylation/125I-streptavidin blotting (Sargiacomo, M., M. P. Lisanti, L. Graeve, A. Le Bivic, and E. Rodriguez-Boulan. 1989 J. Membr. Biol. 107:277-286), in combination with lectin precipitation, to analyze the apical and basolateral glycoprotein composition of Madin-Darby canine kidney (MDCK) cells and to explore the role of glycosylation in the targeting of membrane glycoproteins. All six lectins used recognized both apical and basolateral glycoproteins, indicating that none of the sugar moieties detected were characteristic of the particular epithelial cell surface. Pulse-chase experiments coupled with domain-selective glycoprotein recovery were designed to detect the initial appearance of newly synthesized glycoproteins at the apical or basolateral cell surface. After a short pulse with a radioactive precursor, glycoproteins reaching each surface were biotinylated, extracted, and recovered via precipitation with immobilized streptavidin. Several basolateral ...
Shop Olfactomedin-like protein ELISA Kit, Recombinant Protein and Olfactomedin-like protein Antibody at MyBioSource. Custom ELISA Kit, Recombinant Protein and Antibody are available.
Stanniocalcins (STCs) represent small glycoprotein hormones, found in all vertebrates, which have been functionally implicated in Calcium homeostasis. However, recent data from mammalian systems indicated that they may be also involved in embryogenesis, tumorigenesis and in the context of the latter especially in angiogenesis. Human STC1 is a 247 amino acids protein with a predicted molecular mass of 27 kDa, but preliminary data suggested its di- or multimerization. The latter in conjunction with alternative splicing and/or post-translational modification gives rise to forms described as STC50 and big STC, which molecular weights range from 56 to 135 kDa. In this study we performed a biochemical and structural analysis of STC1 with the aim of obtaining low resolution structural information about the human STC1, since structural information in this protein family is scarce. We expressed STC1 in both E. coli and insect cells using the baculo virus system with a C-terminal 6 × His fusion tag. From the
Sigma-Aldrich offers abstracts and full-text articles by [Jingyuan Su, Bingyu Guo, Tingting Zhang, Kanwen Wang, Xiaoming Li, Guobiao Liang].
PubMed comprises more than 30 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
Synonyms for Glycoproteins in Free Thesaurus. Antonyms for Glycoproteins. 10 words related to glycoprotein: mucin, compound protein, conjugated protein, erythropoietin, CD4, cluster of differentiation 4, CD8.... What are synonyms for Glycoproteins?
This is a protein identified originally in the milk fat globule but shown to be widely distributed in epithelial and mesothelial cells. , 1983). , 1983)have been shown to bind with a high degree of selectivity to tissues of neuroectodermal origin. , 1983). A new TAA, Ca 1, was discovered by Ashall et a2. (1982)and identified as a large molecular weight glycoprotein present in the majority of human tumors. , 1983). , 1983b). These workers used a panel of eight mAbs binding to neuroblastoma and five mAbs binding to leukemia and lymphoma cells to diagnose accurately bone marrow aspirates, frozen sections of tumors and cells from malignant effusions, in cases where conventional techniques yielded uncertain results. 1980). Clin. E r p . Zmmuwl. 39, 90-96. Hill, R . , Silburn, P. , Khoo, S. , and Mackay, E. V. (1983). Cynecol. Oncol. 15, 428-433. Hirohashi, S . , and Ino, Y. (1982a). Br. J. Cancer 46, 802-805. , and Ino, Y. (1982b). Gann 73, 345-347. Holmgren, J . , Rudenstam, C. , and Killander, A. ...
Resources available now: This core will provide collaborations, protocols, materials, and advice on: 1) cell surface capture technology: targeted capture and isolation of glycoproteins located on the cell surface (extracellular exposed plasma membrane proteins) of viable cells. 2) The proteomic analysis for glycoprotein enrichment: targeted capture and isolation of glycoproteins from cells, tissues, and body fluids. 3) Assistance in the development of Multiple Reaction Monitoring (MRM) assays: targeted assays for the quantification of specific glycoproteins. (MRM is a cutting edge method for accurately quantifying many different proteins or glycoproteins in a multiplexed manner). 4) Lectin microarrays and lectin-based immunosorbent assays. Additional resources are in the pipeline. ...
The presence of glycoproteins can be assessed in any material by Schiff staining and additional analysis using Western blot, fluorescent microscopy etc. More exact determination of the glycan nature can be accomplished by lectinoblotting using a range of lectins. The identified proteins can be further purified or identified by mass spectrometry. Also, glycan structures can be determined using mass spectrometry. Cells containing glycoproteins can be specifically labelled by Schiff staining or lectins and enriched using a cell sorter.. ...
It was found previously that transection of one branch of the bifurcate axon of a giant serotonergic neuron of Aplysia causes a proportionate decrease in the export of transmitter storage vesicles from the cell body, despite the ability of the remaining branch to transport all of the vesicles normally exported (Aletta and Goldberg, 1982). We report here evidence that this down-regulation is achieved by a decrease in the production of vesicles, rather than a slowing of the processing of vesicles for export from the cell body. Counts of serotonergic vesicles in the cell body show that the size of the somal pool of vesicles is not increased at a time when their rate of export has been halved. Also, transection of one or both branches of the axon decreases the amount of 3H-fucose incorporated during a short period into a putative vesicle membrane glycoprotein but not into other glycoproteins. The results indicate that axotomy should be a useful technique for studying the mechanism of regulation of ...
The structure of membrane-bound glycoproteins is briefly discussed. Glycoproteins are associated with the cell plasma membrane. Their location on the cell surface has led to suggestions that the...
Glycoproteins are molecules containing sugar and protein, which permit healthy cell-to-cell communication in the human body. Babies receive glycoproteins...
Hourani, B T.; Chace, N M.; and Pincus, J H., "Isolation of membrane glycoproteins from l1210 cells with lectin receptor activities. Abstr." (1973). Subject Strain Bibliography 1973. 653 ...
Each one of these peptides can result in a different VPL motor that can have different properties such as different weight, volume, range of motion, force and speed capabilities. However, the principle of actuation is the same. Studies have shown that the common characteristic in these viruses is the structure of a portion of the surface protein (envelope glycoprotein) and the mode of infection. The envelope glycoproteins of these viruses can be divided into two subunits, which are a result of proteolytic cleavage of a common precursor protein. The two subunits have different functions. For example, in the case of HIV 1 the precursor glycoprotein is gp160, which is proteolytically cleaved into gp120 and gp41 subunits. The gp120 is the surface subunit and the gp41 is the transmembrane (TM) subunit. The surface subunit serves to recognize the cell to be infected when it comes in the vicinity of the virus with the help of receptors located on the cell surface. The gp41 mediates membrane fusion ...
Each one of these peptides can result in a different VPL motor that can have different properties such as different weight, volume, range of motion, force and speed capabilities. However, the principle of actuation is the same. Studies have shown that the common characteristic in these viruses is the structure of a portion of the surface protein (envelope glycoprotein) and the mode of infection. The envelope glycoproteins of these viruses can be divided into two subunits, which are a result of proteolytic cleavage of a common precursor protein. The two subunits have different functions. For example, in the case of HIV 1 the precursor glycoprotein is gp160, which is proteolytically cleaved into gp120 and gp41 subunits. The gp120 is the surface subunit and the gp41 is the transmembrane (TM) subunit. The surface subunit serves to recognize the cell to be infected when it comes in the vicinity of the virus with the help of receptors located on the cell surface. The gp41 mediates membrane fusion ...
GlycoMatrix™ is a new division of bioWORLD. Using our U.S. based manufacturing capability, GlycoMatrix™ has introduced a broad range of the most relevant lectins. Our extensive portfolio contains lectin conjugates for the detection of specific glycoproteins & cancer biomarkers in histological applications, as well
LEGENDplex™ Mouse Cytokine Panel 2 (13-plex) - Cytokines are small soluble glycoproteins that act as extracellular signaling molecuels and meidate communications between cells.
As far as glycoproteins are concerned, at present there is no common international nomenclature. Many investigators have examined them by using various methods of analysis and used more or less...
The carbohydrate protein linkage in glycoproteins. Part I. The synthesis of some model substituted amides and an L -seryl- D -glucosaminide
Semantic Scholar extracted view of [Metabolic disorders of glycoproteins in congenital glycosidase deficiencies]. by K Yamashita
Provided herein are methods, compositions and kits for use in the site-specific labeling of glycoproteins comprising a combination of enzyme-mediated incorporation of modified sugars comprising a chem
... On-line free medical diagnosis assistant. Ranked list of possible diseases from either several symptoms or a full patient history. A similarity measure between symptoms and diseases is provided.
Conventional sample prep techniques for N-glycans can be time-consuming or require compromises in sensitivity. This limits the throughput or detail in which glycoproteins can be characterized or monitored. An innovative label solves those limitations.
1 front of precipitation against rabbit antisera to halpha2-M. Scientific note: Serum glycoprotein of MW approx. 800,000 daltons, containing 38 histidine residues. Two protomers of MW aprox. 370,000 daltons. Each protomer is formed by two sub-units of MW 185,000 daltons, linked by disulfide bridges. N-linked complex carbohydrate side chains ...
Polyclonal antibody for Stanniocalcin 2/STC2 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: WB. Reactive species: Human. Stanniocalcin 2/STC2 information: Molecular Weight: 33249 MW; Subcellular Localization: Secreted ; Tissue Specificity:
Mouse Monoclonal Anti-Stanniocalcin 1/STC-1 Antibody (1A3). Validated: WB, ELISA, ICC/IF. Tested Reactivity: Human. 100% Guaranteed.
Dairpoosh wrote: , Im looking for a method for isolation of Glycoproteins , from plant cells.I would be very grateful if someone can provide , me with a method and a refrence. There is no such thing as *the* method to purify proteins, each protein is different and needs its own combination of methods. Now since you are talking about glycoproteins I presume that you are dealing with a transmembrane protein. To isolate those, you homogenise your cells and isolate the compartment where your protein lives (ER, Golgi, PM, lysosome...). There are standard methods for that, check e.g. Meth. Enzymol. Then you solubilise the membranes with a suitable detergent. There are hundreds of detergents out there, dozens are in common use for membrane protein isolation and may be one or two will solubilise your particular protein without destroying its activity. You have to find out which ones. The following is still the first paper to read, despite being 30 years old: @article{Hel-75, AUTHOR= {A. Helenius and K. ...
There is no size limitation for protein that can be purified with the QProteome Glycoprotein Kits as long as the epitope (the glycan structure) of the glycoprotein is accessible for binding to the immobilized lectin in the purification columns ...
Integrins are transmembrane glycoproteins that belong to the family of adhesion molecules. They promote interactions between cells and their…
Prominin 2兔多克隆抗体(ab74997)可与小鼠, 大鼠, 人样本反应并经WB, ELISA, ICC/IF实验严格验证并得到1个独立的用户反馈。所有产品均提供质保服务,中国75%以上现货。
ウサギ・ポリクローナル抗体 ab47116 交差種: Hu 適用: WB,ELISA…Zinc Alpha 2 Glycoprotein抗体一覧…画像、プロトコール、文献などWeb上の情報が満載のアブカムの Antibody 製品。国内在庫と品質保証制度も充実。
Sugar (glucose) is a key molecule used by all cells as fuel to produce bio-energy and it forms the structural part of glycoproteins and other structur...
a glycoprotein on the outer envelope of HIV that binds to the CD4 protein on the surface of certain cells. The V3 loop of gp120 is the principle neutralizing determinant of HIV and is used in some experimental anti-HIV vaccines ...
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TY - JOUR. T1 - Herpes simplex virus glycoproteins gD and gE/gI serve essential but redundant functions during acquisition of the virion envelope in the cytoplasm. AU - Farnsworth, Aaron. AU - Goldsmith, Kimberly. AU - Johnson, David C.. PY - 2003/8/1. Y1 - 2003/8/1. N2 - The late stages of assembly of herpes simplex virus (HSV) and other herpesviruses are not well understood. Acquisition of the final virion envelope apparently involves interactions between viral nucleocapsids coated with tegument proteins and the cytoplasmic domains of membrane glycoproteins. This promotes budding of virus particles into cytoplasmic vesicles derived from the trans-Golgi network or endosomes. The identities of viral membrane glycoproteins and tegument proteins involved in these processes are not well known. Here, we report that HSV mutants lacking two viral glycoproteins, gD and gE, accumulated large numbers of unenveloped nucleocapsids in the cytoplasm. These aggregated capsids were immersed in an ...
SDS-PAGE studies of EBO virion glycoproteins (Fig. 1 and 3A) and N-terminal sequencing have provided definitive evidence that GP is cleaved (from GP0 form) into two molecules, GP1 and GP2. Cleavage of the structural GP gene product of EBO virus was expected for the N-terminal signal sequence and occurs at the predicted site. Cleavage of GP by furin had been predicted from the RRTRR sequence, but identification of the C-terminal fragment in virions was complicated by its comigration with VP24 in SDS-PAGE. However, prior studies detected GP2 in EBO-infected tissue culture fluids (17, 21), and it was suggested that this uncharacterized glycoprotein might be derived from a cleavage event involving GP. Indirect evidence for furin cleavage of EBO virus GP was recently demonstrated through the use of specific inhibitors of furin activity (21).. When GP sequences of all known EBO viruses are aligned, one finds that the furin cleavage site is conserved but differs slightly from one EBO species to ...
Teramoto, Y A. and Schlom, J, "Radioimmunoassays for the 36,000-dalton glycoprotein of murine mammary tumor viruses demonstrate type, group, and interspecies determinants." (1979). Subject Strain Bibliography 1979. 4658 ...
Fig. 6 OLFML2A and OLFML2B immunoreactivity in human retina. a Confocal image of stained retina sections to identify cells expressing OLFML2A (red, 1st Ab: rabbit polyclonal anti-human OLFML2A 1:500; 2nd Ab: goat anti-rabbit IgG-Cy3® 1:4000). b Confocal image of stained retina seccions to identify cells expressing OLFML2B (red, 1st Ab: rabbit polyclonal anti-human OLFML2B 1:500; 2nd Ab: goat anti-rabbit IgG-Cy3® 1:4000). Cells nuclei were labeled with DAPI (blue). GCL ganglion cell layer, INL inner nuclear layer, ONL outer nuclear layer, PL photoreceptor layer. Discussion. Olfactomedin was originally identified as the major component of the mucus layer that surrounds the chemosen-sory dendrites of olfactory neurons [15]. Subsequently, a vast numbers of proteins that share a ~250 amino acid domain homologous to olfactomedin were discovered in animals ranging from nematodes to humans [4]. One of these proteins was the olfactomedin like 2 proteins. (OLFML2A and OLFML2B), also known as photomedins ...
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gastric mucus glycoproteins: from glandular region of gastric mucosa; high molecular wt. glycoproteins consisting of large numbers of carbohydrate side chains attached to protein core through O-glycosidic linkages; possibly protects stomach from ulceration
The objective of this investigation was primarily to establish that the apparent increase in serum fucose levels in patients with malignant tumors is real and to obtain data as to its source. Carbohydrate residues (L-fucose, D-mannose, D-galactose) were estimated by borate ion-exchange chromatography after mild acid hydrolysis of serum proteins. Data are presented which confirm reported increases in levels of serum fucose in certain malignancies and indicate that these increases may be attributed to quantitative alterations in specific serum glycoproteins observed in the glycoprotein profile associated with cancer. Values for serum fucose levels in pathological and nonpathological sera obtained by various analytical methods are presented and discussed. (Author)
Structure and expression of SPARC (osteonectin, BM-40): a secreted Ca++-binding glycoprotein associated with extracellular matrix production. In: Morphoregulatory Molecules. Eds. Edelman, G.M., Cunnningham, B.A. and Thiery, J.-P ...