A meta-analysis was performed to investigate whether a switch from β-lactams to glycopeptides for cardiac surgery prophylaxis should be advised. Results of 7 randomized trials (5761 procedures) that compared surgical site infections (SSIs) in subjects receiving glycopeptide prophylaxis with SSIs in those who received β-lactam prophylaxis were pooled. Neither agent proved to be superior for prevention of the primary outcome, occurrence of SSI at 30 days (risk ratio [RR], 1.14; 95% confidence interval [CI], 0.91-1.42). In subanalyses, β-lactams were superior to glycopeptides for prevention of chest SSIs (RR, 1.47; 95% CI, 1.11-1.95) and approached superiority for prevention of deep-chest SSIs (RR, 1.33; 95% CI, 0.91-1.94) and SSIs caused by gram-positive bacteria (RR, 1.36; 95% CI, 0.98-1.91). Glycopeptides approached superiority to β-lactams for prevention of leg SSIs (RR, 0.77; 95% CI, 0.58-1.01) and were superior for prevention of SSIs caused by methicillin-resistant gram-positive bacteria ...
TY - JOUR. T1 - Formation of homogeneous cross-linked lattices between oligomannose type glycopeptides and concanavalin A. AU - Islam Khan, M.. AU - Bhattacharyya, Lokesh. AU - Brewer, Curtis F.. PY - 1988/5/16. Y1 - 1988/5/16. N2 - Certain oligomannose type glycopeptides have previously been shown to be bivalent for binding to concanavalin A, and to give quantitative precipitation profiles with the protein that consist of single peaks which correspond to the binding stoichiometry of glycopeptide to protein monomer (1:2) (Bhattacharyya, L., Ceccarini, C., Lorenzoni, P., and Brewer, C.F. (1987) J. Biol. Chem. 262, 1288-1293). In the present study, equimolar mixtures of two oligomannose type glycopeptides, a Man-6 and a Man-9 glycopeptide, gives a quantitative precipitation profile which shows two protein peaks. Each glycopeptide was radiolabelled with 3H or 14C, and the the precipitation profiles of the individual glycopeptides in the mixture determined. The results show that the radioactivity ...
immune Uncategorized Mouse monoclonal to IGFBP2, Ziprasidone manufacture Copeptin, the C-terminal section of provasopressin, has emerged as a novel prognostic marker after hemorrhagic or ischemic stroke. the positive association of copeptin with poor prognosis after stroke was consistent in each stratified analysis. The present meta-analysis suggests that early measurement of plasma copeptin could provide better prognostic information about functional outcome and mortality in Ziprasidone manufacture Mouse monoclonal to IGFBP2 patients with acute stroke. Stroke is a disastrous disease; it is one of the leading causes of death and serious disability worldwide1. Mortality after 1 year ranges between 21% and 27%; approximately 15% to 30% of stroke survivors are permanently disabled1. It is necessary to evaluate prognostic factors to predict functional outcomes and mortality after stroke, which could be effective in creating therapeutic strategies and improving survival rates. Patient age and stroke ...
Four glycopeptides (I, IIA, IIB, III) with different oligosaccharide structures were isolated from purified mouse thymocyte Thy-1 glycoprotein. The glycoprotein was digested with Pronase, and the glycopeptide fraction was isolated by gel filtration and acetylated with [3H]acetic anhydride. The different glycan structures were separated by affinity chromatography on concanavalin A-Sepharose 4B and lentil lectin-Sepharose 4B. Size determinations of intact and exoglycosidase- and endoglycosidase-digested glycopeptides were performed by gel filtration on Bio-Gel P-6, calibrated with glycopeptides of known structure. On the basis of these experiments and on the behaviour of the glycopeptides on the lectin columns, the following structures of the oligosaccharide chains were proposed: I, triantennary complex-type with terminal fucose; IIA, biantennary complex-type without fucose; IIB, biantennary complex-type with fucose; III, a mixture of high-mannose chains containing either five or six ...
Correlation between plasma copeptin levels and other markers used in management of diabatic ketoacidosis in the first 36 hours after diagnosis- Plasma copeptin levels at diagnosis of diabate mellitus type 1 without ketoacidosis in ...
Selective enrichment of glycopeptides from complicated biological samples is essential for MS-based glycoproteomics, but still remains a great challenge. In this study, we report an unprecedented ligand-free strategy for the selective enrichment of glycopeptides by simply utilizing the multivalent interactio
A number of recent advances in the chemical synthesis of glycopeptides and glycoproteins are described, with particular focus on the development of peptide ligation strategies and their implementation in the convergent assembly of complex glycopeptides. Recent applications in the synthesis of full length homogeneou RACI100: Celebrating Australian Chemistry
TY - JOUR. T1 - Edible Birds Nest. T2 - Physicochemical Properties, Production, and Application of Bioactive Extracts and Glycopeptides. AU - Daud, NurAliah. AU - Mohamad Yusop, Salma. AU - Babji, Abdul Salam. AU - Lim, Seng Joe. AU - Sarbini, Shahrul Razid. AU - Hui Yan, Tan. PY - 2019/1/1. Y1 - 2019/1/1. N2 - Edible birds nest (EBN), the swiftlets nest, has been consumed for centuries as a tonic or health food. The information upon the usage and benefits of the EBN are largely based on historical and observational reports since its discovery, until some were proven scientifically at recent times. Studies have been conducted on the major components of the EBN, i.e. glycoprotein, in attempts to elucidate the roles of the glycoprotein in terms of functionality toward health improvement. This article presents a review of the physicochemical and nutritional composition of EBN from different harvested locations and its functional properties. The conversion of EBN glycoproteins to glycopeptides ...
RESULTS: Multivariable linear regression analysis revealed stressful delivery (regression coefficient β = .569, P ,0.001), acidosis (β = -.347, P , .001), and male gender (β = .132, P , .01) as independent determinants of copeptin at birth. In infants born without stress, that is by primary cesarean section (n = 81), male gender was the sole variable associated with copeptin (β = .286, P , .05), copeptin concentrations being higher in boys [median 5.5 pmol/L (interquartile range 4.4-10.2)] than in girls [4.8 pmol/L (interquartile range 3.6-5.8), P , .05]. At day 3 of life, copeptin was determined independently by postnatal physiological dehydration (β = .485; P , .001) and birth weight (β = .279; P , .01 ...
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The goal of the proposed research is to develop a general and highly efficient chemo enzymatic method for making diverse homogeneous glycopeptides and glycoprot...
Synthesis of glycopeptides containing carbohydrate and peptide recognition motifs. Novel enzymatic mechanisms in carbohydrate metabolism
Antimicrobial glycopeptides of innate immune origin : The resistance of insects to bacterial infection is explained by their ability to synthesize a diverse range of antibacterial peptides. A remarkable feature of some proline rich antibacterial peptides such as drosocin, pyrrhocoricin, formaecins, diptericin and lebocins which have been isolated from insects, is the presence of conserved O-glycosylated threonine residue and the integrity of the carbohydrate side chain is necessary for their maximum activity. The exact role of the sugar remains an enigma. We are addressing the problem of role of carbohydrate domains in the activity of antimicrobial glycopeptides by studying their structure-function relationship. However, the mode of action of this class of cationic antimicrobial glycopeptides is not known completely and they are thought to kill bacteria by entering cells and binding to target/targets. The studies addressing mechanistic aspects are also undertaken and are in progress for the ...
To control for potential effects on mortality because of reverse causation, we did a sensitivity analysis excluding patients with major stroke, but results remained qualitatively unchanged (Table 2). In addition, analysis of copeptin by tertiles or by using the 90th percentile of copeptin levels in controls (8.11 pmol/L) in patients revealed similar results (all vascular events: top tertile versus lowest tertile: adjusted HR, 1.91; 95% CI, 1.43-2.53; P,0.0001 and 90th percentile of controls in patients: adjusted HR, 1.76; 1.41-2.20; P,0.0001; Table I in the online-only Data Supplement). Use of the 95th percentile of controls (10.67 pmol/L) in patients revealed practically identical results (all vascular events: adjusted HR, 1.76; 1.40-2.21). The absolute 5-year risk for recurrent vascular events was 0.38 (95% CI, 0.33-0.42) for patients with copeptin levels ≥90th percentile in controls and 0.21 (0.18-0.25) for patients with levels below (Table II in the online-only Data Supplement).. Levels of ...
Fig. 3. Isolation and characterization of N332-dependent antibodies isolated using native-like SOSIP trimers and synthetic Man9-V3 glycopeptide.. (A) ELISA binding analyses of plasma from the HIV-infected donor CH765 to Man9-V3 and aglycone V3 peptides. Donor plasma was screened in duplicate assays, and binding is represented as mean values. (B) Memory B cells from donor CH765 were decorated with either fluorophore-conjugated BG505.T332N.SOSIP and DU156.12.SOSIP in phycoerythrin (PE) and allophycocyanin (APC), or Man9-V3 tetramers tagged to SA-AF647 (Alexa Fluor 647) and SA-BV421 (Brilliant Violet 421). The B cells from which CH765-VRC41.01, VRC41.02, and DH563 were cloned are indicated as green, red, and blue dots, respectively. (C) Immunogenetics and phylogeny of the DH563-VRC41 clonal lineage were inferred using Clonanalyst (33, 34). See also fig. S3. nt, nucleotide. (D) Reactivity of CH765-VRC41.01, CH765-VRC41.02, and DH563 to Man5, Man6, Man7-D1, Man7-D3, Man8-D1D3, and Man9 glycans, ...
A mass spectrometry-based glycoproteomic characterization of zebrafish embryos was performed to identify the N-linked glycoproteins and N-linked glycosylation sites. To increase the number of glycopeptides, proteins from zebrafish were digested with two different proteases--chymotrypsin and trypsin--into peptides of different length. The N-glycosylated peptides of zebrafish were then captured by the solid-phase extraction of N-linked glycopeptides (SPEG) method and the peptides were identified with an LTQ OrbiTrap Velos mass spectrometer.
Waters Glycoprotein BEH Amide 300A, 1.7um columns were primarily designed for the analysis of intact glycoproteins, glycoprotein fragments, and glycopeptides.. Each batch of Glycoprotein BEH Amide 300A 1.7um material is specifically quality control tested with the Waters Glycoprotein Performance Test Standard (P/N 86008010 that includes a mixture of Ribonuclease A as well as Ribonulease B glycoforms) to help ensure batch to batch consistency. This kit includes one Glycoprotein BEH Amide 300A, 1.7um column and one vial of Waters Glycoprotein Performance Test Standard. The standard is included to be able to immediately benchmark the column upon receipt and can be reordered as a separate part for continued monitoring. Each batch of digestion standard is prepared under strict quality control criteria confirmed on the included certificate of analysis.
Waters Glycoprotein BEH Amide 300A, 1.7um columns were primarily designed for the analysis of intact glycoproteins, glycoprotein fragments, and glycopeptides.. Each batch of Glycoprotein BEH Amide 300A 1.7um material is specifically quality control tested with the Waters Glycoprotein Performance Test Standard (P/N 86008010 that includes a mixture of Ribonuclease A as well as Ribonulease B glycoforms) to help ensure batch to batch consistency. This kit includes one Glycoprotein BEH Amide 300A, 1.7um column and one vial of Waters Glycoprotein Performance Test Standard. The standard is included to be able to immediately benchmark the column upon receipt and can be reordered as a separate part for continued monitoring. Each batch of digestion standard is prepared under strict quality control criteria confirmed on the included certificate of analysis.
Chest pain is a clinical problem which often results in emergency department visits and is characterized by difficult differential diagnosis. Although..
This method describes the conjugation of a synthetic glycopeptide to the N-terminus of a recombinant human interleukin-2 (IL-2) protein fragment. The IL-2 protein fragment is produced as an...
Nilsson J, Rüetschi U, Halim A, Hesse C, Carlsohn E, Brinkmalm G, Larson G. Enrichment of glycopeptides for glycan structure and attachment site identification ...
Bubendorf/Basel, October 7, 2014 - Bachem the leading peptide manufacturer, and GlyTech Inc. (Kyoto, Japan), the world-leading developer of glycosylated drugs, announced today that they have entered into a co-promotion agreement for GlyTechs glycosylation technology. As part of the agreement, Bachem will leverage its sales and marketing resources to enhance GlyTechs partnering efforts and GlyTech will promote Bachem as the partner of choice for manufacture of its glycopeptides and glycoproteins. Press release. ...
Cancers Vaccine - Synthetically generated glycopeptides from tumor cells may stimulate the formation of specific antibodies Tumor cells and healthy somatic cells differ in the structures on their surfaces. Because these distinctions between surface area antigens are minimal frequently, all initiatives in this direction have far failed thus; it is not feasible to elicit a targeted immune response directed specifically against the tumor cells. A united group of researchers headed by Horst Kunz in Mainz, Germany, has now come a step further. They have successfully developed a artificial vaccine that induces a solid and particular immune response against the desired tumor antigen in mice.. ...
The Medicines Company (NASDAQ: MDCO) today announced that the U.S. Food and Drug Administration (FDA) has designated oritavancin as a Qualified Infectious Disease Product (QIDP)
Video articles in JoVE about galectin 1 include Isolation and Characterization Of Chimeric Human Fc-expressing Proteins Using Protein A Membrane Adsorbers And A Streamlined Workflow, Isolation and Flow Cytometric Analysis of Glioma-infiltrating Peripheral Blood Mononuclear Cells, Optimized Protocol for the Extraction of Proteins from the Human Mitral Valve, Glycoproteomics of the Extracellular Matrix: A Method for Intact Glycopeptide Analysis Using Mass Spectrometry.
Aim: To explore the association of plasma copeptin, the C-terminal portion of provasopressin and a stable surrogate marker for arginine vasopressin secretion, with plasma glucagon in obese men and men of normal weight. Methods: We measured fasting blood concentrations of copeptin and glucagon in 102 healthy obese men (mean ± sd age 49.4 ± 10.2 years) and a control group 27 healthy men of normal weight (mean ± sd age 51.5 ± 8.4 years). Differences between groups were evaluated using t-tests, and multiple linear regression analysis, adjusting for age and weight status (normal weight vs obese), was used to calculate unstandardized regression coefficients (β) with 95% CIs between copeptin and glucagon. Copeptin was (natural) log-transformed. Results: The obese men had higher [median (interquartile range)] plasma copeptin concentrations [6.6 (4.6-9.5) vs 4.9 (3.5-6.8) pmol/l; P = 0.040] and higher mean ± sd plasma glucagon concentrations (8.5 ± 3.8 vs 5.3 ± 1.4 pmol/l; P , 0.001) than the ...
Background. Copeptin has been known to predict heart failure and cardiovascular death in patients with acute coronary syndrome.. Methods. We collected coronary arterial blood samples from the infarct artery during primary percutaneous coronary intervention (PCI) in 80 STEMI pts and 28 controls. We assessed commonly used cardiac biomarkers (CK, CK-MB, troponin-I, CRP) and additionally measured recently introduced biomarkers (Copeptin [C-terminal Provasopressin] and N-terminal pro-B-type natriuretic peptide [NT-proBNP]).. Results. Pt age was 58±12 yrs and 93% were males. Intravascular ultrasound (IVUS) of the culprit lesion in 67 pts showed ruptured plaques in 28 pts. STEMI pts had a higher Copeptin level (265.89±183.11 pmol/L) vs193.67±60.16 pmol/L in normal controls, p=0.005. Especially, Copeptin levels were higher in ruptured plaque compared to non-ruptured plaques: 318.83±209.34 pmol/L vs 221.99±151.49 pmol/L, p=0.034. While troponin I, CK-MB, and CRP were not correlated with Copeptin, ...
The median concentration of copeptin at admission was lower in AECOPD compared to acute HF (8.8 [5.2-19.7] vs. 22.2 [10.2-47.9]) pmol/L, p < 0.001), but NT-proBNP discriminated acute HF from non-HF related dyspnea more accurately than copeptin (ROC-AUC 0.85 [0.81-0.89] vs. 0.71 [0.66-0.77], p < 0.0001). Adjusted for basic risk factors, increased copeptin concentrations predicted mortality in AECOPD (HR per log (ln) unit 1.72 [95% CI 1.21-2.45], p = 0.003) and acute HF (1.61 [1.25-2.09], p < 0.001), whereas NT-proBNP concentrations predicted mortality only in acute HF (1.62 [1.27-2.06], p < 0.001). On top of a basic model copeptin reclassified a significant proportion of patients into a more accurate risk strata in AECOPD (NRI 0.60 [0.19-1.02], p = 0.004) and acute HF (0.39 [0.06-0.71], p = 0.020). CONCLUSION ...
Crude extracts of Vicia graminea seeds contain an agglutinin that is blood group N specific. The lectin exhibits a complex carbohydrate specificity. It is not inhibited by any mono- or disaccharides, although the O-linked blood group N antigen is a disaccharide with the structure Gal β(1,3)GalNAc. This carbohydrate structure is also recognized by the peanut lectin, but PNA binds to other galactose containing glycoconjugates as well. The peptide portion of the blood group N antigen is critical for binding of VGA. The extended binding site of the lectin reacts with clusters of O-linked Gal β(1,3)GalNAc adjacent to an N-terminal leucine residue 1,2 . Disaccharide clusters located on the first eight amino acids of O-linked glycopeptides react more strongly than these same clusters located on other amino acid residues 2 . VGA will bind to glycoproteins containing sialic acid, but removal of terminal sialic acid residues from these glycoproteins increases the reactivity of VGA. Both VGA and PNA can ...
Chen R., Jiang X., Sun D., Han G., Wang F., Ye M., Wang L., Zou H.. The study of protein glycosylation has lagged far behind the progress of current proteomics because of the enormous complexity, wide dynamic range distribution and low stoichiometric modification of glycoprotein. Solid phase extraction of tryptic N-glycopeptides by hydrazide chemistry is becoming a popular protocol for the analysis of N-glycoproteome. However, in silico digestion of proteins in human proteome database by trypsin indicates that a significant percentage of tryptic N-glycopeptides is not in the preferred detection mass range of shotgun proteomics approach, that is, from 800 to 3500 Da. And the quite big size of glycan groups may block trypsin to access the K, R residues near N-glycosites for digestion, which will result in generation of big glycopeptides. Thus many N-glycosites could not be localized if only trypsin was used to digest proteins. Herein, we describe a comprehensive way to analyze the N-glycoproteome ...
Kathrin Stavenhagen: Glycopeptide analysis remains challenging because of its sample heterogeneity resulting from the degree of glycosylation site occupancy (macroheterogeneity) and the different glycoforms attached to individual glycosylation sites (microheterogeneity).. With respect to the latter one, qualitative site-specific glycosylation information of glycoproteins can be obtained by unspecific protease treatment resulting in small amino acid stretches carrying the glycan. This improves determination of the glycosylation sites. However, detecting these glycopeptides by 1D-LC-ESI-MS/MS is challenging due to insufficient or irreversible retention on the stationary phase and thus multiple analyses with different LC-setups are required. Since biological sample amounts are usually limited, methods for acquiring comprehensive information in a single run are necessary.. To obtain qualitative information of the glycosylation site we set up an integrated C18-porous graphitized carbon ...
Although it typically evades the immune system, HIV does have sites of vulnerability that can be targeted in vaccine design. One such site is a glycan near the V3 loop of the envelope protein, but antibodies recognizing this epitope are often not detected in people infected with HIV. Alam et al. designed a synthetic glycopeptide that can identify B cells targeting this epitope and also used it to immunize macaques. Bonsignori et al. used this synthetic glycopeptide and other baits to study the V3-glycan antibody responses of an HIV-infected individual that developed broadly neutralizing antibodies. They also examined viral evolution over time and found clues as to why these types of antibodies do not develop more often. These tools and findings could pave the way for a vaccine that protects against diverse strains of HIV. ...
1) Kaji H, Saito H, Yamauchi Y, Shinkawa T, Taoka M, Hirabayashi J, Kasai K, Takahashi N, Isobe T. Lectin affinity capture, isotope-coded tagging and mass spectrometry to identify N-linked glycoproteins. Nat Biotechnol. 2003 Jun;21(6):667-72. [PMID: 12754521]. 2) Kaji H, Yamauchi Y, Takahashi N, Isobe T. Mass spectrometric identification of N-linked glycopeptides using lectin-mediated affinity capture and glycosylation site-specific stable isotope tagging. Nat Protoc. 2006;1(6):3019-27. [PMID: 17406563]. 3) Shinkawa T, Taoka M, Yamauchi Y, Ichimura T, Kaji H, Takahashi N, Isobe T. STEM: a software tool for large-scale proteomic data analyses. J Proteome Res. 2005 Sep-Oct;4(5):1826-31. [PMID: 16212438]. 4) Kaji H, Kamiie J, Kawakami H, Kido K, Yamauchi Y, Shinkawa T, Taoka M, Takahashi N, Isobe T. Proteomics reveals N-linked glycoprotein diversity in Caenorhabditis elegans and suggests an atypical translocation mechanism for integral membrane proteins. Mol Cell Proteomics. 2007 Dec;6(12):2100-9. ...
AMI was the final diagnosis in 156 patients (7.9%). A negative copeptin and cTnI at baseline ruled out AMI for 58% of patients, with a negative predictive value (NPV) of 99.2%. AMIs not detected by the initial cTnI alone were picked up with copeptin >14 pmol/L in 23/32 patients (72%). Non−ST-segment elevation MIs (NSTEMIs) undetected by cTnI at 0 hours were detected with copeptin >14 pmol/L in 10/19 patients (53%). Projected average time-to-decision could be reduced by 43% (from 3.0 hours to 1.8 hours) by the early rule out of 58% of patients. Both abnormal copeptin and cTnI were predictors of death at 180 days (p < 0.0001 for both, c-index 0.784 and 0.800, respectively). Both were independent of age and each other and provided additional predictive value (p < 0.0001).. ...
Structural Analysis of Glycosylated Peptides in Complex Mixtures with Ion Trap MS n Shiaw-Lin Wu Pavel Bondarenko Tom Shaler Paul Shieh and William S. Hancock Protein Glycosylation The data presented here can be acquired using a,Structural,Analysis,of,Glycosylated,Peptides,in,Complex,Mixtures,with,,,,,,,,,,,,Ion,Trap,MSn,biological,advanced biology technology,biology laboratory technology,biology device technology,latest biology technology
There is a very strong correlation between the affinity of peptide binding to MHC molecules and the capacity of the peptide to induce an immune response (31, 32). This information is critical to the strategy used in this study. The size of a peptide determines its capacity to bind class I MHC. Whereas the optimal peptide length for most murine MHC molecules appears to be 9-amino acid residues, the Kb and Kk molecules prefer 8-residue peptides (33). Kb molecules were initially chosen to define a glycopeptide that: (a) binds with high affinity; and (b) will be of a structure that skews the T cell response toward recognition of the carbohydrate moiety rather than toward either the peptide or the MHC molecule. The critical anchor residues for Kb binding have been canonically defined as phenylalanine (F) or tyrosine (Y) in position 5 and leucine (L) or methionine (M) at position 8 of an octamer peptide (8). The maintenance of critical hydrogen bonding between main chain atoms in the peptide and atoms ...
Perform Glycan and Glycopeptide Qualitative Analysis, Glycan Structure Prediction & Multi stage Mass Spectrometry (MSn) data analysis using accurate ranking mechanism.
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Seven in one blow: The efficient formation of mixed disulfides on the thiol-rich fusion protein A followed by subsequent intein cleavage gave the fragment B with all seven cysteines protected against oxidation. The native chemical ligation of B with synthetic glycopeptide thioesters provides glycoproteins ...
FDA Accepts Filing of NDA for IV Antibiotic Oritavancin with Priority Review Oritavancin ( INN, also known as LY333328) is a novel semi-synthetic glycopeptide antibiotic being developed for the treatment of serious Gram-positive infections. Originally discovered and developed by Eli Lilly, oritavancin was acquired by InterMune in 2001 and then by Targanta Therapeutics in late 2005. In Dec 2008 the FDA declined to approve […]. ...
FDA Accepts Filing of NDA for IV Antibiotic Oritavancin with Priority Review Oritavancin ( INN, also known as LY333328) is a novel semi-synthetic glycopeptide antibiotic being developed for the treatment of serious Gram-positive infections. Originally discovered and developed by Eli Lilly, oritavancin was acquired by InterMune in 2001 and then by Targanta Therapeutics in late 2005. In Dec 2008 the FDA declined to approve […]. ...
1GAC: Conformation of A82846B, a Glycopeptide Antibiotic, Complexed with its Cell Wall Fragment: An Asymmetric Homodimer Determined Using NMR Spectroscopy.
An estimate of the relative abundance of the various O-glycans was determined from nanoLC-ESI-IT-MS analysis of trypsin-generated (glyco)peptides. It should be noted that because the signal of the monosialylated O-glycopeptide in triply charged state overlapped with the doubly charged peak of the hinge repeat peptide with a putative acetylation modification in all samples, only the doubly charged signal of the monosialylated O-glycopeptide was quantified, thereby leading to an underestimation of this O-glycoform. Furthermore, it is known that glycopeptides with different glycan structures can have different response factors (35), and thus the relative abundances we measured may not accurately reflect the real ratios. In order to obtain a more reliable estimate of the percentage of the hinge repeat motif bearing an O-glycan, tryptic IgG peptides were incubated with exoglycosidases, trimming all O-glycans down to a single N-acetylhexosamine. A previous study of quantitative measurements of a ...
The ovary produces (1) gametes-germ cells in the ovary have undergone the first meiotic division to become oocytes in primordial follicles by the time of birth, with about 400 of these ovulating during reproductive life; and (2) hormones-oestradiol, progesterone, androgens, and two nonsteroidal glycopeptides, inhibin A and B....
Copeptin predicts prognosis in patients with heart failure, according to research presented at the ESC Congress today, August 25, by Professor Stefan Störk from Germany.
192126-76-4 - MQBCDKMPXVYCGO-FQBKTPCVSA-N - Mycothiol - Similar structures search, synonyms, formulas, resource links, and other chemical information.
Tammy Lau - The Importance of Protocol Design for RNA-Seq in the Ongoing Development of a Novel Technology for Tissue Specific Gene Expression Profiling in C. elegans. Arjun Sharma - The Glycopeptide Resistance Predictor. ...
Coagulase-negative staphylococci (CoNS) are a major cause of nosocomial blood stream infection, especially in critically ill and haematology patients. CoNS are usually multidrug-resistant and glycopeptide antibiotics have been to date considered the drugs of choice for treatment. The aim of this study was to characterize CoNS with reduced susceptibility to glycopeptides causing blood stream infection (BSI) in critically ill and haematology patients at the University Hospital Tor Vergata, Rome, Italy, in 2007. Hospital microbiology records for transplant haematology and ICU were reviewed to identify CoNS with elevated MICs for glycopeptides, and isolates were matched to clinical records to determine whether the isolates caused a BSI. The isolates were tested for susceptibility to new drugs daptomicin and tigecycline and the genetic relationship was assessed using f-AFLP. Of a total of 17,418 blood cultures, 1,609 were positive for CoNS and of these, 87 (5.4%) displayed reduced susceptibility to
This unit describes release of oligosaccharides that are attached to polypeptides through an N-acetylgalactosamine (GalNAc) linkage to the hydroxyl groups of serine or threonine. The b-elimination procedures described here can be used to recover the oligosaccharide chains (also called glycans) and/or identify the serine or threonine residues involved in the linkage. A b-elimination method employing sodium borohydride (NaBH4) and alkaline conditions is described, and an alternative method is also presented in which only alkaline conditions are used without a reducing agent. Another alternative protocol uses sodium sulfite. ...
Our group is interested in chemical glycobiology.. Total synthesis is combined with an enzymatic approach to prepare complex carbohydrates, glycopeptides and semi-synthetic glycoproteins. By using synthetic molecules structural details in protein-carbohydrate interactions are explored, which are relevant in infections, inflammation, cancer and aging. In addition to understand disease mechanisms, an ultimate aim in our projects is to develop glycomimetic inhibitors to block specific protein-carbohydrate interactions for instance to address the current problems with antimicrobial resistance in airway disease infections. We further apply synthetic glycopeptides and semi-synthetic glycoproteins to develop glycoproteomic tools for improved enrichment and characterization of glycan structural isomers of glycoproteins and for studies of new PTMs. Small differences in carbohydrate structure has an enormous impact on biological function, for instance in turning on or off an inflammatory pathway and makes ...