Exbio antibodies - Mouse Monoclonal to GAPDHS Hs-8 (IgM)
GAPDHS (the sperm-specific glyceraldehyde phosphate dehydrogenase, also known as GAPD2, GAPDS, HSD-35, or GAPDH-2, is a glycolytic enzyme that plays an important role in carbohydrate metabolism. Like its somatic cell counterpart, this sperm-specific enzyme functions in a nicotinamide adenine dinucleotide-dependent manner to remove hydrogen and add phosphate to glyceraldehyde 3-phosphate to form 1,3-diphosphoglycerate. During spermiogenesis, this enzyme may play an important role in regulating the switch between different energy-producing pathways, and it is required for sperm motility and male fertility. It can be used as an intra-acrosomal marker for evaluation of the physiological state of sperm cells as well as for selection of a suitable method of fertilization in the laboratories of assisted reproduction ...
Differential intron loss and endosymbiotic transfer of chloroplast glyceraldehyde-3-phosphate dehydrogenase genes to the...
Chloroplast glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is composed of two different subunits, GAPA and GAPB, which are encoded in the nucleus by two related genes of eubacterial origin. In the present work the genes encoding chloroplast GAPA and GAPB from pea have been cloned and sequenced. The gene for GAPB is split by eight introns. Two introns interrupt the region encoding the transit peptide and six are found within the region encoding the mature subunit, four of which are in identical or similar positions relative to genes for cytosolic GAPDH of eukaryotic organisms. As opposed to this, the gene encoding pea GAPA has only two introns in the region encoding the mature subunit. These findings strongly support the intron early hypothesis and suggest that the low number of introns in the gene for chloroplast GAPA is due to differential loss of introns during the streamlining period of the chloroplast genome following the GAPB/GAPA separation. We deduce from this that eubacteria and ...
Cloning and Sequence Analysis of Glyceraldehyde-3-Phosphate Dehydrogenase Gene in Yak | Korea Science
Cloning and Sequence Analysis of Glyceraldehyde-3-Phosphate Dehydrogenase Gene in Yak - Yak;Glyceraldehyde-3-Phosphate Dehydrogenase gene;Cloning;Housekeeping Gene;
Characterization of an Arabidopsis mutant deficient in the non-phosphorylating of GAPN gene. - omicX
Non-phosphorylating glyceraldehyde-3-phosphate dehydrogenase (GAPN) is a conserved protein found in higher plants. In photosynthetic cells, the enzyme is involved in a shuttle transfer mechanism to export NADPH from the chloroplast to the cytosol. In this work, we demonstrate that GAPN gene express in leaves and roots at similar levels; showing the highest level of expression in flowers. To investigate the role of this enzyme in different plant tissues, we characterized a mutant from Arabidopsis thaliana having an insertion at the GAPN gene locus. The homozygous mutant was determined to be null respect to GAPN, as it exhibited complete absence of both expression of GAPN mRNA and enzyme activity. Transcriptome analysis demonstrated that the insertion mutant plant shows altered expression of several enzymes involved in carbohydrate metabolism. Significantly, cytosolic phosphorylating (NAD-dependent) glyceraldehyde-3-phosphate dehydrogenase mRNA levels are induced in the mutant, which correlates with an
Recombinant human GAPDH protein (ab82633) | Abcam
Buy our Recombinant human GAPDH protein. Ab82633 is an active full length protein produced in Escherichia coli and has been validated in WB, SDS-PAGE. Abcam…
Energy from Fatty Acid and Glucose | brauch-aktuell.de
Glucose in the bloodstream diffuses into the cytoplasm and is locked there by phosphorylation. A glucose molecule is then rearranged slightly to fructose and phosphorylated again to fructose diphosphate. These steps actually require energy, in the form of two ATPs per glucose. The fructose is then cleaved to yield two glyceraldehyde phosphates (GPs). In the next steps, energy is finally released, in the form of two ATPs and two NADHs, as the GPs are oxidized to phosphoglycerates. One of the key enzymes in this process is glyceraldehyde phosphate dehydrogenase (GPDH), which transfers a hydrogen atom from the GP to NAD to yield the energetic NADH. Due to its key position in the glycolytic pathway, biochemical assays of GPDH are often used to estimate the glycolytic capacity of a muscle cell. Finally, two more ATPs are produced as the phosphoglycerates are oxidized to pyruvate.varicofix pret. ...
NO Deadly Signal | Science Signaling
Reminiscent of mild-mannered Clark Kent, the glycolytic enzyme glyceraldehyde-3-phosphate dehydrogenase (GAPDH) has an alter ego with potent and deadly effects. When translocated to the nucleus, GAPDH has been associated with transcriptional regulation, and Hara et al. present results implicating it in control of apoptosis. A yeast two-hybrid screen for proteins interacting with GAPDH turned up Siah1, an E3 ubiquitin ligase. In transfected cells, the authors detected interaction of the proteins and increased localization of GAPDH to the nucleus, an effect that required the nuclear localization signal of Siah1. In human embryonic kidney cells undergoing apoptosis in response to staurosporine, the authors detected modification of GAPDH by mass spectrometry and went on to show in vitro that modification of GAPDH by S-nitrosylation (often a consequence of increased generation of nitric oxide within cells) enhanced association with Siah1. These events appear to be important in regulation of ...
Gapdh - Glyceraldehyde-3-phosphate dehydrogenase - Rattus norvegicus (Rat) - Gapdh gene & protein
Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules. Also participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes.
Tyrosine nitration and altered protein pool levels of glyceraldehyde-3-phosphate dehydrogenase following ischemia/reperfusion:...
Aging and skeletal muscle ischemia/reperfusion (I/R) injury both lead to skeletal\r\nmuscle dysfunction, evidenced by decreased contractile force generation, particularly in\r\nglycolytic muscle. The deficits in I/R are more severe and persistent in aged animals.\r\nPrevious studies in our lab led us to hypothesize that the expression of the glycolytic\r\nenzyme glyceraldehyde-3-phosphate dehydrogenase may be altered following I/R. We\r\nfurther hypothesized that aging would enhance the oxidative stress and oxidative damage\r\nexperienced by the muscle. GAPDH protein levels were measured by Western blotting.\r\nWe observed that the enzyme is significantly decreased at 3 and 5 days of reperfusion in\r\nthe young muscle, while the enzyme was significantly decreased in the aged muscle at 1,\r\n3, 5, and 7 days. Using PCR, we compared GAPDH mRNA levels at 5 days reperfusion\r\nand found that the I/R tissue from both young and old have significant increases in\r\nGAPDH transcript at this time point ...
GAPDHS (glyceraldehyde-3-phosphate dehydrogenase, spermatogenic)
GAPDHS (glyceraldehyde-3-phosphate dehydrogenase, spermatogenic), Authors: Dessen P. Published in: Atlas Genet Cytogenet Oncol Haematol.
Secreted multifunctional Glyceraldehyde-3-phosphate dehydrogenase sequesters lactoferrin and iron into cells via a non...
Lactoferrin is a crucial nutritionally important pleiotropic molecule and iron an essential trace metal for all life. The current paradigm is that living organisms have evolved specific membrane anchored receptors along with iron carrier molecules for regulated absorption, transport, storage and mobilization of these vital nutrients. We present evidence for the existence of non-canonical pathway whereby cells actively forage these vital resources from beyond their physical boundaries, by secreting the multifunctional housekeeping enzyme Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) into the extracellular milieu. This effects an autocrine/paracrine acquisition of target ligand into the cell. Internalization by this route is extensively favoured even by cells that express surface receptors for lactoferrin and involves urokinase plasminogen activator receptor (uPAR). We also demonstrate the operation of this phenomenon during inflammation, as an arm of the innate immune response where ...
Glyceraldehyde-3-phosphate dehydrogenase gene over expression correlates with poor prognosis in non small cell lung cancer...
Glyceraldehyde-3-phosphate dehydrogenase gene over expression correlates with poor prognosis in non small cell lung cancer patients. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
Glyceraldehyde-3-phosphate dehydrogenase
Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in ...
Glyceraldehyde-3-phosphate dehydrogenase-catalyzed chain oxidation of reduced nicotinamide adenine dinucleotide by perhydroxyl...
The chain oxidation of glyceraldehyde-3-phosphate dehydrogenase.NADH by perhydroxyl radicals and propagated by molecular oxygen was studied by the xanthine-xanthine oxidase system, 60Co gamma-ray, and pulse radiolysis. The chain length, amount of NADH oxidized per HO2 generated, increases with increasing acidity of the medium and reaches a value of 73 at pH 5.0. The rate constant for the oxidation of the glyceraldehyde-3-phosphate dehydrogenase.NADH complex by HO2 was estimated to be 2 X 10(7) M-1 S-1 at ambient temperatures (23-24 degrees C). Rate studies as a function of pH indicate that O2- is unreactive toward the glyceraldehyde-3-phosphate dehydrogenase.NADH complex. Other dehydrogenases (malate dehydrogenase, glutamate dehydrogenase, and isocitric dehydrogenase) studied showed no catalytic activity in the oxidation of NADH by HO2/O2-. ...
siSTABLE GAPD Control siRNA - Mouse
siSTABLE GAPD Control siRNA is a validated positive control, guaranteed to silence GAPD in mouse cells. This control siRNA is chemically modified to significantly extend siRNA stability and is recommended for use as a positive control in experiments using siSTABLE modified siRNA against a target gene or where increased stability of the control is desired.. Also known as glyceraldehyde-3-phosphate dehydrogenase or GAPDH, GAPD is an important enzyme in carbohydrate metabolism that is well conserved across the animal kingdom. This gene is abundantly expressed in most cells, and because it is non-essential, knockdown of the corresponding mRNA does not affect cell viability. Targets accession number NM_001001303.. ...
siGENOME GAPD Control siRNA
siGENOME GAPD Control siRNA is a validated positive control, guaranteed to silence GAPD in human cells. siGENOME siRNA positive control designs are highly functional, chemically synthesized and mostly unmodified siRNA duplexes that allow positive silencing for validation of experimental design and detection methodologies.. Also known as glyceraldehyde-3-phosphate dehydrogenase or GAPDH, GAPD is an important enzyme in carbohydrate metabolism that is well conserved across the animal kingdom. This gene is abundantly expressed in most cells and because it is non-essential, knockdown of the corresponding mRNA does not affect cell viability. ...
The use of live antigens, listeriolysin O (LLO) and glyceraldehyde-3-phosphate-dehydrogenase (GAPDH), - HDAC Inhibition for the...
The use of live antigens, listeriolysin O (LLO) and glyceraldehyde-3-phosphate-dehydrogenase (GAPDH), and several epitopes such as the LLO peptides, LLO189?201 and LLO91?99 and the GAPDH peptide, GAPDH1?22. vaccines against various other pathogens or in cancers therapy. Nevertheless, because it is normally a individual virus, it might trigger life-threatening attacks such as serious meningitis, encephalitis, and human brain abscess in pregnant females, neonates, aging adults people, and immunocompromised people. Vaccination is normally one of the most effective strategies to deal with contagious illnesses. Nevertheless, in the complete case of listeriosis, there is normally no vaccine obtainable for high-risk groupings such as newborns, pregnant females, or people with immunological disability. Current research of prophylactic vaccines against concentrate on three strategies: (i) the creation of live attenuated pathogens capable to gain access to the cytosol and induce Testosterone levels cells, ...
Plastidial Glycolytic Glyceraldehyde-3-Phosphate Dehydrogenase Is an Important Determinant in the Carbon and Nitrogen...
This study functionally characterizes the Arabidopsis (Arabidopsis thaliana) plastidial glycolytic isoforms of glyceraldehyde-3-phosphate dehydrogenase (GAPCp) in photosynthetic and heterotrophic cells. We expressed the enzyme in gapcp double mutants (gapcp1gapcp2) under the control of photosynthetic (Rubisco small subunit RBCS2B [RBCS]) or heterotrophic (phosphate transporter PHT1.2 [PHT]) cell-specific promoters. Expression of GAPCp1 under the control of RBCS in gapcp1gapcp2 had no significant effect on the metabolite profile or growth in the aerial part (AP). GAPCp1 expression under the control of the PHT promoter clearly affected Arabidopsis development by increasing the number of lateral roots and having a major effect on AP growth and metabolite profile. Our results indicate that GAPCp1 is not functionally important in photosynthetic cells but plays a fundamental role in roots and in heterotrophic cells of the AP. Specifically, GAPCp activity may be required in root meristems and the root ...
Gapdhs - Glyceraldehyde-3-phosphate dehydrogenase - Rattus norvegicus (Rat) - Gapdhs gene & protein
p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class=publication>Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href=http://www.nrbook.com/b/bookcpdf.php>Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...
Glyceraldehyde-3-Phosphate Dehydrogenase, Spermatogenic (GAPDHS) Antikörper
Reaktivität: Hund, Meerschweinchen, Pferd and more. verschiedene GAPDHS Antikörper vergleichen. Alle direkt auf antikörper-online bestellbar!
Characterization and possible function of glyceraldehyde-3-phosphate dehydrogenase-spermatogenic protein GAPDHS in mammalian...
Sperm proteins are important for the sperm cell function in fertilization. Some of them are involved in the binding of sperm to the egg. We characterized the acrosomal sperm protein detected by a monoclonal antibody (MoAb) (Hs-8) that was prepared in our laboratory by immunization of BALB/c mice with human ejaculated sperms and we tested the possible role of this protein in the binding assay. Indirect immunofluorescence and immunogold labelling, gel electrophoresis, Western blotting and protein sequencing were used for Hs-8 antigen characterization. Functional analysis of GAPDHS from the sperm acrosome was performed in the boar model using sperm/zona pellucida binding assay. Monoclonal antibody Hs-8 is an anti-human sperm antibody that cross-reacts with the Hs-8-related protein in spermatozoa of other mammalian species (boar, mouse). In the immunofluorescence test, Hs-8 antibody recognized the protein localized in the acrosomal part of the sperm head and in the principal piece of the sperm flagellum. In
Native Bakers yeast (S. cerevisiae) Glyceraldehyde-3-phosphate Dehydrogenase(EC 1.2.1.12) - Creative Enzymes
Glyceraldehyde-3-phosphate dehydrogenase catalyzes the conversion of glyceraldehyde-3-phosphate to 1,3-bisphosphoglycerate as part of glycolysis. It has also been shown t
Anti-GAPDH Mouse Monoclonal Antibody (2B5), HRP Conjugated - Abbkine - Antibodies, proteins, biochemicals, assay kits for life...
Featured HRP Conjugated Anti-GAPDH Mouse Monoclonal Antibody (2B5) , specially designed for your immunoassay as internal control.,GAPDH; GAPD; CDABP0047; OK/SW-cl.12; Glyceraldehyde-3-phosphate dehydrogenase; GAPDH; Peptidyl-cysteine S-nitrosylase GAPDH,Glyceraldehyde 3-phosphate dehydrogenase (abbreviated as GAPDH or less commonly as G3PDH) is an enzyme of ~37kDa that catalyzes the sixth step of glycolysis and thus serves to break down glucose for energy and carbon molecules. In addition to this long established metabolic function, GAPDH has recently been implicated in several non-metabolic processes, including transcription activation, initiation of apoptosis ER to Golgi vesicle shuttling, and fast axonal, or axoplasmic transport.
RCSB PDB - 1NQA: Glyceraldehyde-3-Phosphate Dehydrogenase Mutant With Cys 149 Replaced By Ala Complexed With Nad+ and...
1NQA: Crystal structure of two ternary complexes of phosphorylating Glyceraldehyde-3-Phosphate Dehydrogenase from Bacillus stearothermophilus with NAD and D-Glyceraldehyde-3-Phosphate
Glucose-6-phosphate dehydrogenase (G6PD) synonyms, glucose-6-phosphate dehydrogenase (G6PD) antonyms - FreeThesaurus.com
Synonyms for glucose-6-phosphate dehydrogenase (G6PD) in Free Thesaurus. Antonyms for glucose-6-phosphate dehydrogenase (G6PD). 8 words related to glucose: aldohexose, glucosamine, corn sugar, dextroglucose, dextrose, grape sugar, blood glucose, blood sugar. What are synonyms for glucose-6-phosphate dehydrogenase (G6PD)?
28S-ribosomal RNA is superior to glyceraldehyde-3-phosphate dehydrogenase as a RNA reference gene in p53-deficient mice with...
28S-ribosomal RNA is superior to glyceraldehyde-3-phosphate dehydrogenase as a RNA reference gene in p53-deficient mice with unilateral ureteral obstruction Academic Article ...
Glyceraldehyde 3-phosphate dehydrogenase - Wikipedia
Glyceraldehyde 3-phosphate dehydrogenase (abbreviated as GAPDH or less commonly as G3PDH) (EC 1.2.1.12) is an enzyme of ~37kDa that catalyzes the sixth step of glycolysis and thus serves to break down glucose for energy and carbon molecules. In addition to this long established metabolic function, GAPDH has recently been implicated in several non-metabolic processes, including transcription activation, initiation of apoptosis, ER to Golgi vesicle shuttling, and fast axonal, or axoplasmic transport. In sperm, a testis-specific isoenzyme GAPDHS is expressed. Under normal cellular conditions, cytoplasmic GAPDH exists primarily as a tetramer. This form is composed of four identical 37-kDa subunits containing a single catalytic thiol group each and critical to the enzymes catalytic function. Nuclear GAPDH has increased isoelectric point (pI) of pH 8.3-8.7. Of note, the cysteine residue C152 in the enzymes active site is required for the induction of apoptosis by oxidative stress. Notably, ...
Plus it
The main result of this study was that GAPDH activity was decreased in embryos subjected to a teratogenic diabetic environment in vivo (maternal diabetes) or diabetes-like environment in vitro (increased glucose concentration). When the disturbed embryonic development in vitro was corrected by the addition of the antioxidant NAC to the culture medium with high glucose concentration, GAPDH activity increased toward normal values. This strongly suggests the existence of common ROS-associated elements in the pathogenesis of glucose-induced congenital malformations and glucose-induced inhibition of embryonic GAPDH activity.. To probe the relation among glucose-induced embryonic maldevelopment, GAPDH inhibition, and ROS excess, we removed the glucose challenge by directly inhibiting GAPDH with IA. We found that IA supplementation to the culture medium yielded embryonic maldevelopment similar to the glucose-induced dysmorphogenesis and, furthermore, that the addition of NAC diminished both the GAPDH ...
VARIANTS OF THE PROMOTER OF THE GAP GENE CODING FOR GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE - diagram, schematic, and...
VARIANTS OF THE PROMOTER OF THE GAP GENE CODING FOR GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE - diagram, schematic, and image 47 ...
Structural basis for the regulation of endothelin-1 mRNA stability by glyceraldehyde-3-phosphate dehydrogenase :: UMBC...
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) plays a key role in vascular homeostasis by modulating expression levels of the vasoconstrictor endothelin-1 (ET-1). Elevated ET-1 levels are associated with vascular diseases, and ET-1 expression is tightly regulated via GAPDH-mediated destabilization of its mRNA. Recent studies suggest GAPDH binding of adenine-uridine rich elements (AREs) in the 3-untranslated region (3-UTR) of the ET-1 gene leads to decreased mRNA stability. However, structural and mechanistic details underlying the GAPDH-mediated control of ET-1 expression are lacking. We sought to elucidate the structural and mechanistic details for the GAPDH-induced destabilization of ET-1 mRNA via electrophoretic mobility shift assay (EMSA), isothermal titration calorimetry (ITC), and x-ray crystallography. To identify specific GAPDH binding sequences, we constructed short RNA transcripts of the putative mRNA binding region. Utilizing ITC and EMSA, we obtained dissociation constants. We ...
Effects of Th2 Cytokines on Expression of Collagen, MMP-1, and TIMP-1 in Conjunctival Fibroblasts | IOVS | ARVO Journals
Total RNA was extracted from cells cultured in the monophasic solution of phenol and guanidine isothiocyanate (TRIzol reagent; Life Technologies-GibcoBRL, Milan, Italy). Briefly, the cells were lysed by addition of 1.0 mL of extraction reagent, and total RNA was subsequently isolated according to the manufacturers instructions. Complementary DNA was synthesized from 500 ng total RNA per sample with 50 minutes incubation at 37°C, using Moloney murine leukemia virus reverse transcriptase (Life Technologies-Gibco BRL) and oligo (dT) priming. Amplification was performed in a programmable thermal controller (PTC-100; MJ Research Inc., Watertown, MA), with recombinant Taq DNA polymerase (Applied Biosystems, Foster City, CA) and the specific primer pairs reported in Table 1 . The parallel amplification of cDNA for the housekeeping enzyme glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used as an internal control. To enable a semiquantitative comparison between samples, serial threefold ...
RCSB PDB - 1JN0: Crystal structure of the non-regulatory A4 isoform of spinach chloroplast glyceraldehyde-3-phosphate...
1JN0: Crystal structure of the non-regulatory A(4 )isoform of spinach chloroplast glyceraldehyde-3-phosphate dehydrogenase complexed with NADP.
Mouse mAb anti- Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), Clone H8 | esiservizi.com
Product Name: Mouse mAb anti- Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), Clone H8Collection: AntibodySub Category: Monoclonal AntibodyImmunogen:
glyceraldehyde-3-phosphate dehydrogenase S homeolog ELISA Kits | Biocompare
Compare glyceraldehyde-3-phosphate dehydrogenase S homeolog ELISA Kits from leading suppliers on Biocompare. View specifications, prices, citations, reviews, and more.
Dymocks - Glyceraldehyde-3-phosphate Dehydrogenase Gapdh by Michael A. Sirover
Buy Glyceraldehyde-3-phosphate Dehydrogenase Gapdh from Dymocks online BookStore. Find latest reader reviews and much more at Dymocks
Anti Human GAPDH Antibody, clone 4G5 | Bio-Rad
|strong|Mouse anti Human GAPDH antibody, clone 4G5|/strong| recognizes glyceraldehyde-3-phosphate dehydrogenase (GAPDH), a 36 kDa protein whose main function is to catalyse the reversible oxidative ph…
GAPDH Gene - GeneCards | G3P Protein | G3P Antibody
Complete information for GAPDH gene (Protein Coding), Glyceraldehyde-3-Phosphate Dehydrogenase, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
protein groups list
GAPB (GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE B SUBUNIT); glyceraldehyde-3-phosphate dehydrogenase (NADP+)/ glyceraldehyde-3-phosphate dehydrogenase ...
3dmt.2 | SWISS-MODEL Template Library
SWISS-MODEL Template Library (SMTL) entry for 3dmt.2. Structure of Glycosomal Glyceraldehyde-3-Phosphate Dehydrogenase from Trypanosoma cruzi in complex with the irreversible iodoacetate inhibitor
glyceraldehyde-3-phosphate dehydrogenase
(= GAPD; GAPDH; G3PD) Glycolytic enzyme (EC 1.2.1.12) that catalyses the reversible oxidative phosphorylation of glyceraldehyde 3 phosphate. Has been shown to interact with various elements of the cytoskeleton, and with the trinucleotide repeat…
Various rat adult tissues express only one major mRNA species from the glyceraldehyde-3-phosphate-dehydrogenase multigenic...
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OriGene - GAPDH (NM 002046) cDNA Clone
GAPDH - GAPDH (untagged)-Human glyceraldehyde-3-phosphate dehydrogenase (GAPDH) available for purchase from OriGene - Your Gene Company.
Glucose-6-phosphate dehydrogenase (G6PD). Response of the human erythrocyte and another cells to the decrease in their activity...
TY - JOUR. T1 - Glucose-6-phosphate dehydrogenase (G6PD). Response of the human erythrocyte and another cells to the decrease in their activity. AU - Bonilla, Javier Fernando. AU - Sánchez, Magna Carolina. AU - Chuaire, Lilian. PY - 2007/1/1. Y1 - 2007/1/1. N2 - Glucose-6-phosphate dehydrogenase is the first enzyme in the pentose phosphate pathway and the main intracellular source of reduced nicotidamineadenine nucleotidephosphate (NADPH), involved in diverse physiological processes such as antioxidant defense, (for instance in the erythrocyte) endothelial growth modulation, erithropoyesis, vascularization and phagocitosis, G6PDH deficiency is the most common X-chromosome-linked enzymopathy in human beings. Although it is present in any type cell, its absolute deficiency is incompatible with life. According to WHO, 400 million people are affected by G6PD deficiency in the world but in Colombia, the severe form prevalence is about 3% to 7%. There are no data related to slight and moderate ...
Apoptosis and DNA Damage (H2A.X(S139) + cleaved PARP1 + Anti-GAPDH) Western
Anti-GAPDH抗体(ab22555)参考文献| Abcam中国官方网站
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Metabolic and transcriptional response of Escherichia coli with a NADP+-dependent glyceraldehyde 3-phosphate dehydrogenase from...
The NAD+-dependent glyceraldehyde-3-phosphate-dehydrogenase (NAD+-GAPDH) is a key enzyme to sustain the glycolytic function in Escherichia coli and to generate NADH. In the absence of NAD+-GAPDH activ
Gentaur Molecular :Alpha Dia \ Anti Rabbit muscle Glyceraldehyde 3 Phosphate Dehydrogenase G3PDH IgG 2 \ G3PDH12-S
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gapdh Summary [species: Xenopus laevis and Xenopus tropicalis] - Xenbase Gene Catalog
Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Modulates the organization and assembly of the cytoskeleton. Also participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins. [-] ...
Housekeeping gene validation - Real-Time PCR
im running qPCR on two cell lines (equine oral and limb fibroblasts) treated with various treatments. i have run all treatments for each cell line with my GAPDH primers and examination of the delta c(t) for each separate cell line indicates that GAPDH expression is not affected by treatment but when you put the data for both sets of cell lines together, there is a significant change in expression between the two. ie, expression of GAPDH is not altered by treatment in either of the cell lines, but it is expressed at different ...
Transferrin gene expression and transferrin immunolocalization in developing foetal rat lung<...
TY - JOUR. T1 - Transferrin gene expression and transferrin immunolocalization in developing foetal rat lung. AU - Skinner, S. J.M.. AU - Somekvell, C. E.. AU - Buch, S.. AU - Post, M.. PY - 1991. Y1 - 1991. N2 - In previous studies we have shown that transferrin (Tf) specifically stimulates dermatan- and chondroitin-sulphate proteoglycan accumulation around lung cells, and in the extracellular matrix of lung tissue, in vitro. The aim of this study was to determine whether the gene for Tf was activated in specific lung cells during development, and whether the protein product showed evidence of association with extracellular matrix. The expression of the gene in developing lung was shown by the hybridization of a Tf cDNA to a 2.4 kb (kilobase) mRNA species in total RNA extracts of foetal lung. The expression of the Tf gene in comparison to a control gene (GAPD, glyceraldehyde phosphate dehydrogenase) was greatest in 19, 20 and 21 day foetal lung, rising from low levels on day 18 and decreasing ...
Mycobacterium tuberculosis acquires iron by cell-surface sequestration and internalization of human holo-transferrin - UQ eSpace
Mycobacterium tuberculosis (M.tb), which requires iron for survival, acquires this element by synthesizing iron-binding molecules known as siderophores and by recruiting a host iron-transport protein, transferrin, to the phagosome. The siderophores extract iron from transferrin and transport it into the bacterium. Here we describe an additional mechanism for iron acquisition, consisting of an M.tb protein that drives transport of human holo-transferrin into M.tb cells. The pathogenic strain M.tb H37Rv expresses several proteins that can bind human holo-transferrin. One of these proteins is the glycolytic enzyme glyceraldehyde-3-phosphate dehydrogenase (GAPDH, Rv1436), which is present on the surface of M.tb and its relative Mycobacterium smegmatis. Overexpression of GAPDH results in increased transferrin binding to M.tb cells and iron uptake. Human transferrin is internalized across the mycobacterial cell wall in a GAPDH-dependent manner within infected macrophages ...
Nuclear Localization of Overexpressed Glyceraldehyde-3-Phosphate Dehydrogenase in Cultured Cerebellar Neurons Undergoing...
Figure 2 Localization of overexpressed GAPDH in apoptotic CGCs detected by using subcellular fractionation and Western blotting. A, Subcellular fractionation of immature CGCs (20-24 hr after plating) exposed to ara-C for 24 hr. B, Subcellular fractionation of mature CGCs exposed to low K+ for 12 hr. Subcellular fractions from various CGC samples were prepared, resolved by SDS-PAGE, electroblotted onto polyvinylidene difluoride membranes, and reacted with a monoclonal antibody for the overexpressed GAPDH as described in Materials and Methods. Measurement of level of GAPDH protein on the autogram also is described in the text. Bar graphs, levels of quantified GAPDH proteins expressed as relative values compared with the unexposed control (C). Values are mean ± standard error of three independent experiments. ∗, p , 0.05; ∗∗, p , 0.01 compared with the corresponding untreated (vehicle) control in each fraction using Studentst test. V, Plus vehicle (i.e., H2O). A, Plus Act-D (1 μg/ml).X, ...
Detection of UCP1 protein and measurements of dependent GDP-sensitive proton leak in non-phosphorylating thymus mitochondria. -...
Over several years we have provided evidence that uncoupling protein 1 (UCP1) is present in thymus mitochondria. We have demonstrated the conclusive evidence for the presence of UCP1 in thymus mitochondria and we have been able to demonstrate a GDP-sensitive UCP1-dependent proton leak in non-phosphorylating thymus mitochondria. In this chapter, we show how to detect UCP1 in mitochondria isolated from whole thymus using immunoblotting. We show how to measure GDP-sensitive UCP1-dependent oxygen consumption in non-phosphorylating thymus mitochondria and we show that increased reactive oxygen species production occurs on addition of GDP to non-phosphorylating thymus mitochondria. We conclude that reactive oxygen species production rate can be used as a surrogate for detecting UCP1 catalyzed proton leak activity in thymus mitochondria.
Inhibition of glyceraldehyde-3-phosphate dehydrogenase and other glycolytic enzymes by acrylamide<...
TY - JOUR. T1 - Inhibition of glyceraldehyde-3-phosphate dehydrogenase and other glycolytic enzymes by acrylamide. AU - Sabri, M. I.. AU - Spencer, P. S.. PY - 1980/1/1. Y1 - 1980/1/1. UR - http://www.scopus.com/inward/record.url?scp=0018932962&partnerID=8YFLogxK. UR - http://www.scopus.com/inward/citedby.url?scp=0018932962&partnerID=8YFLogxK. M3 - Article. AN - SCOPUS:0018932962. VL - 19. SP - S455. JO - Neuroscience Letters. JF - Neuroscience Letters. SN - 0304-3940. IS - SUPPL. 5. ER - ...
Glycerol-3-Phosphate Dehydrogenase Activity Colorimetric Assay Kit - Creative Proteomics
Creative Proteomics offer Glycerol-3-Phosphate Dehydrogenase Activity Colorimetric Assay Kit. We are specialized in manufacturing Assay Kits.
Aberrant Intracellular pH Regulation Limiting Glyceraldehyde-3-Phosphate Dehydrogenase Activity in the Glucose-Sensitive Yeast...
The addition of glucose to tps1Δ cells of the yeast S. cerevisiae causes hyperaccumulation of all glycolytic metabolites upstream and depletion of all metabolites downstream of GAPDH, suggesting that the deletion of Tps1 in some way creates a bottleneck in glycolysis at the level of GAPDH (27). Measurements of the specific activity of the glycolytic enzymes in cell extracts as well as determination of initial glucose uptake rates did not reveal significant differences between the wild-type and tps1 strains that could explain the glycolytic bottleneck in tps1Δ cells (1, 11). More detailed measurements of the glucose uptake rate and the pH dependency of GAPDH in the present work have underscored the conclusion that there is no difference in the inherent activity of these two crucial components in the tps1Δ strain. Hence, the bottleneck appears to be due to a metabolic or regulatory problem at the level of GAPDH that is not maintained in cell extracts and is not apparent from the Vmax or Km of ...
Protein recognition of the S23906-1-DNA adduct by nuclear proteins: direct involvement of glyceraldehyde-3-phosphate...
In a view to develop new DNA alkylating antitumour drugs, evaluating the precise mechanism of action and the molecular/cellular consequences of the alkylation is a point of major interest. The benzo-b-acronycine derivative S23906-1 alkylates guanine nucleobases in the minor groove of the DNA helix and presents an original ability to locally open the double helix of DNA, which appears to be associated with its cytotoxic activity. However, the molecular mechanism linking adduct formation to cellular consequences is not precisely known. The objective of the present study was to identify proteins involved in the recognition and mechanism of action of S23906-DNA adducts. We found that GAPDH (glyceraldehyde-3-phosphate dehydrogenase) is a protein that binds to S23906-alkylated single-stranded, double-stranded and telomeric sequences in a drug-dependent and DNA sequence/structure-dependent manner. We used the CASTing (cyclic amplification of sequence targeting) method to identify GAPDH DNA-binding ...
Alpha-enolase in viral target cells suppresses the human immunodeficiency virus type 1 integration | Retrovirology | Full Text
A protein exhibiting more than one biochemical function is termed a moonlighting protein. Glycolytic enzymes are typical moonlighting proteins, and these enzymes control the infection of various viruses. Previously, we reported that glyceraldehyde 3-phosphate dehydrogenase (GAPDH) and alpha-enolase (ENO1) are incorporated into human immunodeficiency virus type 1 (HIV-1) particles from viral producer cells and suppress viral reverse transcription independently each other. However, it remains unclear whether these proteins expressed in viral target cells affect the early phase of HIV-1 replication. Here we show that the GAPDH expression level in viral target cells does not affect the early phase of HIV-1 replication, but ENO1 has a capacity to suppress viral integration in viral target cells. In contrast to GAPDH, suppression of ENO1 expression by RNA interference in the target cells increased viral infectivity, but had no effect on the expression levels of the HIV-1 receptors CD4, CCR5 and CXCR4 and on
Browse our anti-Glucose-6-Phosphate Dehydrogenase
Order monoclonal and polyclonal Glucose-6-Phosphate Dehydrogenase antibodies for many applications. Selected quality suppliers for anti-Glucose-6-Phosphate Dehydrogenase antibodies.
Corrections to Intermetallic GaPd2 Nanoparticles on SiO<sub>2</sub> for Low-Pressure CO<sub>2</sub> Hydrogenation to Methanol:...
TY - JOUR. T1 - Corrections to Intermetallic GaPd2 Nanoparticles on SiO2 for Low-Pressure CO2 Hydrogenation to Methanol: Catalytic Performance and In Situ Characterization. AU - Fiordaliso, Elisabetta Maria. AU - Sharafutdinov, Irek. AU - Carvalho, Hudson W. P.. AU - Grunwaldt, Jan-Dierk. AU - Hansen, Thomas W.. AU - Chorkendorff, Ib. AU - Wagner, Jakob Birkedal. AU - Damsgaard, Christian Danvad. PY - 2018. Y1 - 2018. U2 - 10.1021/acscatal.7b04342. DO - 10.1021/acscatal.7b04342. M3 - Comment/debate. VL - 8. SP - 938. EP - 938. JO - A C S Catalysis. JF - A C S Catalysis. SN - 2155-5435. ER - ...
Glyceraldehyde 3 Phosphate Dehydrogenase Gapdh Abcam | Bioz | Ratings For Life-Science Research
Search Results for Glyceraldehyde 3 Phosphate Dehydrogenase Gapdh Abcam on Bioz, providing objective ratings for all products used in life science research.
Glyceraldehyde 3 Phosphate Dehydrogenase Gapdh Millipore | Bioz | Ratings For Life-Science Research
Search Results for Glyceraldehyde 3 Phosphate Dehydrogenase Gapdh Millipore on Bioz, providing objective ratings for all products used in life science research.
Profile | Biosciences | University of Exeter
The crystal structure of the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) from the archaeon Methanothermus fervidus has been solved in the holo form at 2.1 a resolution by molecular replacement. Unlike bacterial and eukaryotic homologous enzymes which are strictly NAD(+)-dependent, GAPDH from this organism exhibits a dual-cofactor specificity, with a marked preference for NADP(+) over NAD(+). The present structure is the first archaeal GAPDH crystallized with NADP(+). GAPDH from M. fervidus adopts a homotetrameric quaternary structure which is topologically similar to that observed for its bacterial and eukaryotic counterparts. Within the cofactor-binding site, the positively charged side-chain of Lys33 decisively contributes to NADP(+) recognition through a tight electrostatic interaction with the adenosine 2-phosphate group. Like other GAPDHs, GAPDH from archaeal sources binds the nicotinamide moiety of NADP(+) in a syn conformation with respect to the adjacent ribose and so belongs to ...
Cytokine mRNA expression in lesions in cats with chronic gingivostomatitis
Semiquantitative reverse transcription-PCR assays were developed to measure feline interleukin-2 (IL-2), IL-4, IL-5, IL-6, IL-10, and IL-12 (p35 & p40); gamma interferon (IFN-gamma); and glyceraldehyde-3-phosphate dehydrogenase mRNA concentrations in biopsies of feline oral mucosa. Biopsies were …
IJMS | Free Full-Text | A Differential Redox Regulation of the Pathways Metabolizing Glyceraldehyde-3-Phosphate Tunes the...
Adaptation to aerobic life leads organisms to sense reactive oxygen species and use the signal for coordination of the entire metabolism. Glycolysis in plants is a particular network where specific steps, like oxidation of glyceraldehydes-3-phosphate (Ga3P), are critical in order for it to function. The triose-phosphate can be converted into 3-phosphoglycerate through the phosphorylating Ga3P dehydrogenase (Ga3PDHase, EC 1.2.1.12) producing ATP and NADH, or via the non-phosphorylating enzyme (np-Ga3PDHase; EC 1.2.1.9) generating NADPH. In this work we found redox regulation to be a posttranslational mechanism allowing the fine-tuning of the triose-phosphate fate. Both enzymes were inactivated after oxidation by reactive oxygen and nitrogen species. Kinetic studies determined that Ga3PDHase is marked (63-fold) more sensitive to oxidants than np-Ga3PDHase. Thioredoxin-h reverted the oxidation of both enzymes (although with differences between them), suggesting a physiological redox regulation. The
SERCA - casein kinases mediate the phosphorylatable protein pp49
Supplementary MaterialsSupplementary Info. HPV16+/p53WT HNSCC but not in HPV?/p53HRmut HNSCC. Knockdown of the dominant ALDH isoform in high AVS HNSCC depleted the CIC pool and limiting dilution assay in NSG mice showed that ALDH1A3 knockdown dramatically depleted the CIC population by? ?60-fold in Y-27632 2HCl distributor UMSCC47. CIC frequency was reduced from 1/9,205 to 1/590,453 (p? ?0.001). Open in a separate window Figure 5 Targeting the dominant ALDH isoform in high AVS HNSCC depletes the CIC pool. UMSCC47 and SCC25 cells were transduced with the inducible pLV-RNAi/shRNA-ALDH1A3 and polyclonal cell populations were collected. Cells were stimulated with doxycycline at 1000?ng/ml for all the experiments. (a) ALDH1A3 protein levels. Cell lysates were immunoblotted with anti-ALDH1A3 and GAPDH antibodies. Representative image is cropped. (b) ALDH1A3 mRNA expression. ALDH1A3 and GAPDH expression was determined using qPCR with TaqMan primers. Data were normalized to GAPDH and are presented as ...
The E3 ubiquitin-ligase SEVEN IN ABSENTIA like 7 mono-ubiquitinates glyceraldehyde-3-phosphate dehydrogenase 1 isoform in vitro...
The E3 Ubiquitin-protein Ligases Are Associated To Various Processes Such As Cell Cycle Control And Diverse Developmental Pathways. Arabidopsis Thaliana SEVEN IN ABSENTIA Like 7, Which Has Ubiquitin Ligase Activity, Is Located In The Nucleus And Cytosol
Dual Coenzyme Specificity of Photosynthetic Glyceraldehyde-3-phosphate Dehydrogenase Interpreted by the Crystal Structure of A ...
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Glyceraldehyde-3-phosphate dehydrogenase mediates anoxia response and survival in Caenorhabditis elegans | Genetics
The Genetics Society of America (GSA), founded in 1931, is the professional membership organization for scientific researchers and educators in the field of genetics. Our members work to advance knowledge in the basic mechanisms of inheritance, from the molecular to the population level.. Online ISSN: 1943-2631. ...
GPD - sn-glycerol 3-phosphate dehydrogenase by AcronymsAndSlang.com
What does GPD stand for? Hop on to get the meaning of GPD. The Acronym /Abbreviation/Slang GPD means sn-glycerol 3-phosphate dehydrogenase. by AcronymAndSlang.com
Kinetic mechanism from steady-state kinetics of the reaction catalysed by bakers-yeast glucose 6-phosphate dehydrogenase in...
1. The reaction catalysed by glucose 6-phosphate dehydrogenase (D-glucose 6-phosphate-NADP+ oxidoreductase, EC 1.1.1.49) from bakers yeast was studied in 42mM-glycylglycine buffer, pH7.4 at 25 degrees C, by initial-velocity studies and by the use of NADPH as a product inhibitor. 2. The reactions catalysed by both the soluble enzyme and a stable enzyme covalently attached to CNBr-activated Sepharose 4B probably follow an ordered reaction mechanism with NADP+ and NADPH as the leading reactants. 3. The kinetic constants obtained for the soluble enzyme lere: KNADP+m, 19 muM; KNADP+s, 23 muM; KNADPHs, 15 muM. Similar values were obtained for the immobilized enzyme. 4. The assay of the immobilized enzyme was done by using a micro packed-bed recirculation reactor, and the advantages of this technique are discussed. ...
Rat G6PD(Glucose 6 Phosphate Dehydrogenase) ELISA Kit - Bio EMM
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Rat Glucose 6 Phosphate Dehydrogenase (G6PD) in samples from Serum, plasma, tissue homogenates and other biological fluids. with no significant corss-reactivity with analogues from other species ...
In vitro effect of metal ions on the activity of two amphibian...
In vitro effect of metal ions on the activity of two amphibian glyceraldehyde-3-phosphate dehydrogenases: potential metal binding sites.: Glyceraldehyde-3-phosp
GAPDS monoclonal antibody (M01), clone 2E3-2E10 - (H00026330-M01) - Products - Abnova
Mouse monoclonal antibody raised against a full length recombinant GAPDS. GAPDS (AAH36373, 1 a.a. ~ 408 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. (H00026330-M01) - Products - Abnova
GAPDH primer sequences for mRNA level in HeLa cells - Real-Time PCR
Can someone share the sequences of a pair of primers that has been SUCCESSFULLY employed for the determination of the GAPDH (glyceraldehyde-3-phosphate dehydrogenase) mRNA level in human cells (HeLa) by the real-time PCR method with the SYBR Green I detection ...
Material-driven fibronectin assembly for high-efficiency presentation of growth factors | Science Advances
Fig. 2 Integrin/BMP-2 receptor cosignaling drives MSC osteogenesis.. (A) Coimmunoprecipitation of integrin β1 and BMPRI occurred on BMP-2 sequestered by FN on PEA, and bands correspond to BMPRIa (60 kD) after precipitation with anti-integrin β1 antibodies. The graphs show quantification of bands relative to the absence of BMP-2. This colocalization can also be seen in individual cells with integrin β1 (stained red) and BMPRIa (stained green). (B) Smad signaling was drastically altered when BMP-2 was presented bound on FNIII12-14; blocking this GF-binding domain of FN (using the monoclonal antibody P5F3 at a molar ratio of 1 with FN to block the GF-binding site) reduces Smad signaling. GAPDH, glyceraldehyde-3-phosphate dehydrogenase. (C) Phosphorylation of extracellular signal-related kinase (ERK) 1/2 was significantly enhanced on PEA when BMP-2 was presented at the material interface, sequestered on FN, compared to the presence of the same doses of the soluble factor. (D) In-cell Western ...
Quantitative analysis of mRNA in human temporal bones
COCH mRNA could be amplified as much as 976 bp in all three frozen specimens. By contrast, it was amplified to 249 bp in two of the three formalin-fixed specimens, with no amplification observed in the remaining. The quantity of amplifiable GAPDH mRNA in the formalin specimens was only 1% of that of …
Measure oxidative metabolism and glycolytic activity
Learn how to measure oxidative metabolism and glycolytic activity on the SpectraMax i3x reader using assays from Agilent Technologies.
Korean Journal of Microbiology (한국미생물학회지) | Korea Science
보다 유용함이 밝혀졌다. 이 두가지 affinity media에 흡착된 효소를 분리 하는데 가장 적합한 elution 조건을 조사하였는데 KCI gradient( (0-1.OM)가 효소의 순도 및 수회율을 가장 높일 수 있는 적합한 방법이었다. 특히 Affi-gel Blue를 사용할 경우, KCI gradient로 효소를 용출시키기 전에 NAD-(15mM)로NAD+에 친화역을 갖는 효소들을 제거하는 것이 enzyme의 순도를 높이는데 매우 효과적이었다. 그 결과 glucose-6-phosphate dehydrogenase를 bakers yeast로 부터 기존의 간단한 정제 과정과 affinity chromatography를 병행한 방식을 샤용하여 분리 하였는데, affinity medium으로 Affi-gel Blue를 사용했을 때는 180배 정도, NADP+-agarose를 사용했을 때는 2,000배 정도로 정제 되었다. 대량으로 glucose-6-phosphate dehydrogenase를 정제하는 경우, Affi-gel Blue를 사용하던 효소의 순도는 NADP+-agarose보다 낮으나, 효소의 회수율은 ...
Hormonal regulation of malic enzyme and glucose-6-phosphate dehydrogenase in adult rat liver | Biochemical Society Transactions
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WikiGenes - G6PD - glucose-6-phosphate dehydrogenase
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Národní úložiště šedé literatury
2018 - English Non-healing wounds are serious complication in diabetic patients and represent an attractive challenge for development of suitable carrier system possessing constant and localized release of therapeutic biomolecule into the wound without any undesired side effects. Given the fact that these non-healing wounds are result of impaired balance in metalloproteinases synthesized by immune cells residing the wounds, gene therapy offering knock down of such enzymes is of great interest. \nHere we challenged a development of functional and biocompatible wound dressing enabling controlled release of trackable carrier loaded with therapeutic siRNA. Our dressing consists of scaffold from degradable polymer nanofibers enriched with fluorescent nanodiamond particles (FND). We have previously shown the nanodiamond particles are great carriers for antisense RNAs. Their advantages represent high biocompatibility, stable luminescence giving us the possibility to track the carrier system in the ...
Národní úložiště šedé literatury
2016 - Czech Nedávné studie ukázaly že neplodnost v lidské populaci postihuje odhadem 15% párů v reprodukčním věku. Mužská neplodnost je primární příčinou u 60% těchto případů. Z těchto důvodů jsme analyzovali povrchové a akrozomální proteiny spermií u mužů s normálním a patologickým spermiogramem. Zjistili jsme že intra-akrozomální proteiny: TERA, GAPDHS, PRKAR2A, které lze analyzovat pomocí našich monoklonálních protilátek , jsou rozdílně exprimovány u zdravých mužů a mužů s asthenozoospermií a to se signifikantně sníženou expresí u asthenozoospermie. tyto proteiny se účastní energetického metabolismu a apoptózy buněk a některé z nich i vazby na vajíčko - mají tedy důležitou roli v reprodukci. Na druhou starnu nebyly zjisštěny statisticky významné rozdíly v expresi povrchových proteinů (Appolipoprotein J, Semenogelin). Naše závěry ukazují že asthenozoospermie jako komplexní poruch apermatu je často v kombinaci s ...
Which controls can I choose for FlexiPlate siRNA? - QIAGEN
You can add the following controls to your FlexiPlate siRNA plate: AllStars Negative Control siRNA, AllStars Cell Death Control siRNA, Negative Control siRNA, Human GAPDH siRNA, Human Beta-Actin siRNA, Human and mouse MAPK1 siRNA, Human or mouse Lamin A/C siRNA, Mouse AKT1 siRNA, or other siRNAs from GeneGlobe, such as HP Validated siRNAs. ...
VCAC: Cellular Processes: Glycolysis - An Overview: The Movie -- Narrative
This key process takes place in the cytosol of the cell.. Lets take a look at an overview of the key steps of glycolysis and focus on the input and output molecules. The first five steps of glycolysis convert a 6-carbon sugar (glucose) into two 3-carbon sugars (glyceraldehyde-3-phosphate). To do so, ATP is consumed in the first and third steps. Both of these chemical reactions are catalyzed by an enzyme known as a kinase, and both reactions are irreversible ...
Publications of Salma Balazadeh | Max Planck Institute of Molecular Plant Physiology
Moreno, J.C.; Rojas, B.E.; Vicente, R.; Gorka, M.; Matz, T.; Kosmacz, M.; Peralta-Ariza, J.S.; Zhang, Y.; Alseekh, S.; Childs, D. et al.; Luzarowski, M.; Nikoloski, Z.; Zarivach, R.; Walther, D.; Hartman, M.D.; Figueroa, C.M.; Iglesias, A.A.; Fernie, A. R.; Skirycz, A.: Tyr-Asp inhibition of glyceraldehyde 3-phosphate dehydrogenase affects plant redox metabolism. EMBO Journal, e106800 (2021 ...
PDB 2qcu structure summary ‹ Protein Data Bank in Europe (PDBe) ‹ EMBL-EBI
2qcu: Structure of glycerol-3-phosphate dehydrogenase, an essential monotopic membrane enzyme involved in respiration and metabolism.
Ceveron® s100 incl. PC - Dialine
Intended use The Trinity Biotech Glucose-6-Phosphate Dehydrogenase kit is for the quantitative, ultraviolet, kinetic determination of G-6-PDH in blood at ...
Study suggests starve cancer of energy with natural molecule -- Science & Technology -- Sott.net
Cancers energy source can be thwarted by a naturally-occurring molecule which stops affected cells from consuming sugar, according to a new study. Koningic acid (KA) was found to disrupt the metabolism of cancerous cells. Published in Cell...