TY - JOUR. T1 - Glutathione S-transferase pi isoform (GSTP1) expression in murine retina increases with developmental maturity. AU - Lee, Wen Hsiang. AU - Joshi, Pratibha. AU - Wen, Rong. PY - 2014. Y1 - 2014. N2 - Glutathione S-transferase pi isoform (GSTP1) is an intracellular detoxification enzyme that catalyzes reduction of chemically reactive electrophiles and is a zeaxanthin-binding protein in the human macula. We have previously demonstrated that GSTP1 levels are decreased in human age-related macular degeneration (AMD) retina compared to normal controls (Joshi et al., Invest Ophthalmol Vis Sci, e-abstract, 2009). We also showed that GSTP1 levels parallel survival of human retinal pigment epithelial (RPE) cells exposed to ultraviolet (UV) light, and GSTP1 over-expression protects them against UV light damage (Joshi et al., Invest Ophthalmol Vis Sci, e-abstract, 2010). In the present work, we determined the developmental time course of GSTP1 expression in murine retina and in response to ...

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Purpose: : Glutathione S-transferase pi isoform (GSTP1) is an intracellular detoxification enzyme that catalyzes reduction of chemically reactive electrophiles and is a zeaxanthin-binding protein in the human macula. We have previously demonstrated that GSTP1 levels are decreased in human age-related macular degeneration (AMD) retina compared to normal controls. We also showed that GSTP1 levels parallel survival of human retinal pigment epithelial (RPE) cells exposed to UV light, and GSTP1 over-expression protects them against UV light damage. In the present work, we determined the developmental time course of GSTP1 expression in murine retina and in response to light challenge. Methods: : The eyes from BALB/c mice at post-natal day 20, 1 month, and 2 months of age were prepared for retinal protein extraction and for cryo sectioning, and the GSTP1 levels in the retina were analyzed by Western blot and by immunohistochemistry (IHC), respectively. Another group of BALB/c mice with the same age ...

Transcriptional regulation of glutathione S-transferase P1-1 in human leukemia: Expression of glutathione S-transferase P1-1 (GSTP1-1) is correlated to carcinog

GST Pi is a member of the glutathione-S-transferase superfamily of phase II xenobiotic-metabolizing enzymes that catalyse the conjugation of endogenous and exogenous electrophiles, including reactive oxygen species, toxins, carcinogens and anti-cancer agents, to the nucleophilic thiol group of reduced glutathione (GSH) [12]. A number of studies have concentrated on the connection between aberrant expression of GST isozymes, including GST Pi isozymes, with the development and expression of resistance to chemotherapy drugs as reviewed by McIlwain et al.[13]. From this perspective an expected result should have been a poor response to chemotherapy in patients with high expression of GST Pi. However our study has shown that, for stage C colon cancer patients, overall survival was significantly and markedly poorer in patients with high GST Pi who did not receive chemotherapy than in those with high GST Pi who did. Furthermore, survival in the latter group was no different from that in patients with ...

Genetic variations in the detoxification enzyme glutathione S-transferase P1 (GSTP1) may modify the teratogenicity of lifestyles, such as smoking. We investigated the role of the I105V polymorphism in GSTP1, parental periconception smoking, and their interaction with nonsyndromic cleft lip with or without cleft palate (CL/P) risk in the offspring. The GSTP1 I105V polymorphisms were determined in Dutch non-consanguineous Caucasians comprising of 155 CL/P triads (mother, father, child) and 195 control triads. The analyses were also carried out on complete triads only (n=69 CL/P and n=95 controls). Transmission disequilibrium testing and logistic regression analyses were performed. Neither maternal nor paternal smoking increased CL/P risk; odds ratios (OR): 1.2, 95 confidence intervals (CI)=0.7-2.0 and OR: 1.0, 95% CI=0.6-1.6, respectively. Carriership of the polymorphic Val105 allele in mothers may increase CL/P risk, OR: 1.5, 95% CI=0.96-2.5. Children homozygous for the Val105 allele may show an ...

2015 Elsevier Inc. High-dose chemotherapy regimens using cyclophosphamide (CY) are frequently associated with cardiotoxicity that could lead to myocyte damage and congestive heart failure. However, the mechanisms regulating the cardiotoxic effects of CY remain unclear. Because CY is converted to an unsaturated aldehyde acrolein, a toxic, reactive CY metabolite that induces extensive protein modification and myocardial injury, we examined the role of glutathione S-transferase P (GSTP), an acrolein-metabolizing enzyme, in CY cardiotoxicity in wild-type (WT) and GSTP-null mice. Treatment with CY (100-300. mg/kg) increased plasma levels of creatine kinase-MB isoform (CK·MB) and heart-to-body weight ratio to a significantly greater extent in GSTP-null than WT mice. In addition to modest yet significant echocardiographic changes following acute CY-treatment, GSTP insufficiency was associated with greater phosphorylation of c-Jun and p38 as well as greater accumulation of albumin and protein-acrolein ...

An association between glutathione S-transferase P1 gene polymorphism and younger age at onset of lung carcinoma. Cancer. 2006 Oct 01; 107(7):1570-7 ...

GST pi, the main glutathione S-transferase isoform present in the human brain, was isolated from various regions of the brain and the in vitro effect of tricyclic antidepressants on its activity was studied. The results indicated that amitripyline and doxepin - derivatives of dibenzcycloheptadiene, as well as imipramine and clomipramine - derivatives of dibenzazepine, inhibit the activity of GST pi from frontal and parietal cortex, hippocampus and brain stem. All these tricyclics are noncompetitive inhibitors of the enzyme with respect to reduced glutathione and noncompetitive (amitripyline, doxepin) or uncompetitive (imipramine, clomipramine) with respect to the electrophilic substrate. Their inhibitory effect is reversible and it depends on the chemical structure of the tricyclic antidepressants rather than on the brain localization of the enzyme. We conclude that the interaction between GST pi and the drugs may reduce their availability in the brain and thus affect their therapeutic activity. ...

Glutathione-S-transferase P1 (GSTP1) is a critical enzyme of the phase II detoxification pathway. One of the common functional polymorphisms of GSTP1 is A→G at nucleotide 313, which results in an amino acid substitution (Ile105Val) at the substrate binding site of GSTP1 and reduces catalytic activity of GSTP1.

A member of the glutathione-S-transferase family, glutathione-S-transferase P1 (GSTP1), is involved in susceptibility to carcinogen-associated colorectal cancer.. An A to G transition in exon 5 of the glutathione-S-transferase P1 gene resulting in Ile105Val amino acid substitution has been identified.. This change leads to alteration in catalytic efficiency of variant enzyme.. Dr Tatyana Vlaykova and colleagues from Bulgaria evaluated the influence of Ile105Val glutathione-S-transferase P1 polymorphism on susceptibility to colorectal cancer.. The team conducted the glutathione-S-transferase P1 genotyping in a case-control study of 80 ethnic Bulgarian colorectal cancer patients. In addition, the investigators assessed 126 unaffected controls using polymerase chain reaction restriction fragment length polymorphism method.. The investigative team observed a statistically significant case-control difference in genotype frequencies.. The difference in genotype frequencies were 0.7 vs 0.5 for Ile/Ile, ...

Role of Glutathione-S-Transferase P1 genetic variants in the etiopathogeneses of type 2 Diabetes Mellitus and its effect on the glycemic control ...

10GS: The structures of human glutathione transferase P1-1 in complex with glutathione and various inhibitors at high resolution.

The association between glutathione S-transferase pi (GSTpi) and other clinicopathological parameters, response to chemotherapy and clinical outcome were investigated in chemotherapy naive epithelial ovarian cancer patients. Paraffin-embedded material from 55 patients were used for immunohistochemic …

BACKGROUND: Air pollutants may induce airway inflammation and sensitization due to generation of reactive oxygen species. The genetic background to these mechanisms could be important effect modifiers. OBJECTIVE: Our goal was to assess interactions between exposure to air pollution and single

Protein S-glutathionylation is a reversible post-translational modification regulating sulfhydryl homeostasis. However, little is known about the proteins and pathways regulated by S-glutathionylation in whole organisms and current approaches lack the sensitivity to examine this modification under basal conditions. We now report the quantification and identification of S-glutathionylated proteins from animal tissue, using a highly sensitive methodology combining high-accuracy proteomics with Tandem Mass Tagging to provide precise, extensive coverage of S-glutathionylated targets in mouse liver. Critically, we show significant enrichment of S-glutathionylated mitochondrial and Krebs cycle proteins, identifying that S-glutathionylation is heavily involved in energy metabolism processes in vivo . Furthermore, using mice nulled for glutathione S-transferase Pi (GSTP) we address the potential for S-glutathionylation to be mediated enzymatically. The data demonstrate the impact of S-glutathionylation ...

Emerging evidence of risk factors such as infectious agents and environmental factors have been proposed to precede the development of PIN lesions (De Marzo et al, 1999; De Marzo et al, 2007). Epidemiological studies also suggested that chronic inflammation and/or recurrent infection might contribute to prostate carcinogenesis (Grivennikov et al, 2010; Karin & Greten, 2005; Karin et al, 2006). Proliferative inflammatory atrophy (PIA) lesions of the glandular epithelial foci could be a connection between prostatitis and PIN or PCa (De Marzo et al, 2007; Palapattu et al, 2005). The inflammatory lesions in the aging prostate are frequently associated with atrophic epithelium and some fractions of epithelium undergo active proliferation, which may develop into PIN or PCa. In the molecular aspects, several genetic alteration such as glutathione S-transferase pi 1 (GSTP1), NKX3.1, p27 and PTEN have been regarded as the markers of PIN and PCa (Wagenlehner et al, 2007). Collectively, this ...

We report for the first time label-free quantification of xenobiotic metabolizing enzymes (XME), transporters, redox enzymes, proteases and nucleases in six human skin explants and a 3D living skin equivalent model from LabSkin. We aimed to evaluate the suitability of LabSkin as an alternative to animal testing for the development of topical formulations. More than 2000 proteins were identified and quantified from total cellular protein. Alcohol dehydrogenase 1C (ADH1C), the most abundant phase I XME in human skin, and glutathione S-transferase pi 1 (GSTP1), the most abundant phase II XME in human skin, were present in similar abundance in LabSkin. Several esterases were quantified and esterase activity was confirmed in LabSkin using substrate-based mass spectrometry imaging. No cytochrome P450 (CYP) activity was observed for the substrates tested, in agreement with the proteomics data, where the cognate CYPs were absent in both human skin and LabSkin. Label-free protein quantification allowed ...

GSTP1 antibody (glutathione S-transferase pi 1) for ICC/IF, IHC-P, WB. Anti-GSTP1 pAb (GTX112953) is tested in Human, Mouse, Rat samples. 100% Ab-Assurance.

Antigens; Neoplasm/genetics, DNA; Mitochondrial/genetics, DNA-Binding Proteins/genetics, E2F3 Transcription Factor/genetics, Epigenesis; Genetic/genetics, Gene Expression Regulation; Neoplastic, Genetic Markers, Genome; Human/genetics, Glutathione S-Transferase pi/genetics, Humans, Male, Methylation, Microsatellite Repeats/genetics, Mutation, Prostate-Specific Antigen/genetics, Prostatic Neoplasms/*diagnosis/*genetics, RNA; Messenger/analysis, Racemases and Epimerases/genetics, Reverse Transcriptase Polymerase Chain Reaction, Serine Endopeptidases/genetics, Telomerase/genetics, Transcription Factors/genetics, Tumor Markers; Biological, Tumor Suppressor Proteins ...

ABCA formed a novel intramolecular cross-linking adduct on human serum albumin in patients and in vitro via Michael addition, followed by nucleophilic adduction of the aldehyde with a neighbouring protein nucleophile. Adducts were detected on lysine, histidine, and cysteine residues in subdomain IB of human serum albumin. Only a cysteine adduct and a putative cross-linking adduct were detected on glutathione S-transferase Pi. Modelling the docking of ABCA with HLA B*57:01 confirmed that ABCA has a strong binding affinity when bound covalently to Ser116, a key residue with regards to recognition by ABC-specific CD8+ T cells. ...

Huang, J., Tan, P.-H., Thiyagarajan, J., Bay, B.-H. (2003). Prognostic significance of glutathione S-transferase-pi in invasive breast cancer. Modern Pathology 16 (6) : 558-565. ScholarBank@NUS Repository. https://doi.org/10.1097/01.MP.0000071842.83169. ...

The major findings of this study are that deletion of the GSTP gene exacerbates endothelial dysfunction induced by exposure to tobacco smoke or to inhaled acrolein. We hypothesized that smoking-induced endothelial dysfunction was mediated in part by electrophilic constituents of CS such as acrolein. Hence, detoxification of electrophilic CS constituents by GSTP may be a protective mechanism against the vascular effects of tobacco smoke and other acrolein-rich pollutants (e.g., coal smoke, wood smoke, and automobile exhausts). The results obtained support the hypothesis and significantly advance our understanding of the vascular toxicity of tobacco smoke and the processes that modulate the CVD risk of smoking.. The endothelium appears to be a highly vulnerable target of tobacco smoke. Smoking injures endothelial cells, and vessels isolated from chronic smokers show degenerative changes (10). In humans, smoking diminishes flow-mediated endothelium-dependent vasodilation (5), and serum from smokers ...

We have investigated levels of transcript homologous with glutathione S-transferase P (GST-P; GST 7-7) in tumours and hyperplastic lesions induced in the livers of rats by long-term gavage dosing with diethylnitrosamine (DEN) and 6-p-dimethylaminophenylazobenzothiazole (6BT). Detailed histopathological examination of the livers of the 90 animals used in this study at 6-8 months after initiation of daily dosing revealed that, of the 30 animals treated with carcinogen, 15 had developed tumours or hyperplastic lesions. Of these, 11 were areas of fibrosarcoma/fibrous hyperplasia. The remaining four were hepatocellular carcinomas. Northern blotting of total RNA purified from these tissues revealed the presence of transcripts of 3 and 0.75 kb. Evidence is presented to indicate that the former is a hitherto-undetected precursor of the 3-kbp rat GST-P gene, the latter representing the previously characterized mature GST-P transcript. Large elevations of the 0.75-kb transcript (30-35-fold) were ...

To our knowledge, this is the first report on the association between GSTP1 genotype and clinical outcome following chemotherapy in Hodgkins lymphoma. Our results are in line with reports on an association between 105Val GSTP1 allele and a favorable prognosis in other malignancies (12-15). The impact of the GSTP1 genotype is, therefore, probably not specific for a tumor type but may be related to the metabolism of anticancer drugs. Patients with the 105Val GSTP1 variant have a reduced ability to detoxify chemotherapeutic agents, which determines an increase in the effective dose of the drug within the cell. Our data are compatible with a gene dosage effect. Watson et al. (11) showed that individuals with two GSTP1 105Val alleles have a lower catalytic activity when compared with individuals with two GSTP1 105Ile alleles, whereas an intermediate activity was reported for heterozygotes.. The precise mechanism whereby GSTP1 is responsible for resistance to anticancer agents is still matter of ...

TY - JOUR. T1 - Nitrosylation of human glutathione transferase P1-1 with dinitrosyl diglutathionyl iron complex in vitro and in vivo. AU - Cesareo, Eleonora. AU - Parker, Lorien J.. AU - Pedersen, Jens Z.. AU - Nuccetelli, Marzia. AU - Mazzetti, Anna P.. AU - Pastore, Anna. AU - Federici, Giorgio. AU - Caccuri, Anna M.. AU - Ricci, Giorgio. AU - Adams, Julian J.. AU - Parker, Michael W.. AU - Lo Bello, Mario. PY - 2005/12/23. Y1 - 2005/12/23. N2 - We have recently shown that dinitrosyl diglutathionyl iron complex, a possible in vivo nitric oxide (NO) donor, binds with extraordinary affinity to one of the active sites of human glutathione transferase (GST) P1-1 and triggers negative cooperativity in the neighboring subunit of the dimer. This strong interaction has also been observed in the human Mu, Alpha, and Theta GST classes, suggesting a common mechanism by which GSTs may act as intracellular NO carriers or scavengers. We present here the crystal structure of GST P1-1 in complex with the ...

Cell confluence induces resistance to chemotherapeutic agents in solid tumor cells and phosphorylation of heat shock protein 27 (HSP27), a low-weight molecular chaperon and stress response protein. Here, we show that human glutathione S-transferase P1 (GSTP1) inhibits phosphorylation of HSP27 induced by both epidermal growth factor (EGF) stimulation and cell confluence in malignant brain tumor cells, resulting in enhancement of resistance to cisplatin. Using the paired human medulloblastoma cell lines, UW228 (GSTP1-ve) and UW228-GSTP1 (GSTP1+ve), we showed that growth at high cell density (confluence) induced resistance to cisplatin, regardless of GSTP1 status. Western blotting analysis of extracts of GSTP1-ve cells growing at high density or stimulated with EGF stimulation showed significantly higher phosphorylation levels of HSP27, whereas the HSP27 phosphorylation was inhibited in GSTP1+ve cells. Interestingly, the induction of HSP27 phosphorylation in GSTP1-ve cells was independent of HSP27 ...

5J41: Structural and Biochemical Analyses Reveal the Mechanism of Glutathione S-Transferase Pi 1 Inhibition by the Anti-cancer Compound Piperlongumine.

Many phytochemicals possess antioxidant and cancer-preventive properties, some putatively through antioxidant response elementCmediated phase II rate of metabolism, entailing mutagen/oxidant quenching. moderate, and none was apparent for glutathione S-transferase pi proteins manifestation. Measurements of reactive air varieties and glutathione/oxidized glutathione percentage demonstrated an antioxidant impact for DMEBP, but no certain effect was discovered for TRES in NHBE cells. Publicity of NHBE cells to H2O2 induced nuclear translocation of nuclear element erythroid 2Crelated element 2, but this translocation had not been inhibited by TRES and DMEBP considerably. These studies also show that strength and low toxicity may for just two potential NQO1-inducing real estate agents align, TRES and DMEBP. screening method of display 800 substances in an all natural items library, this scholarly research offers determined 2,3-dihydroxy-4-methoxy-4-ethoxybenzophenone (derivative, myrtle extract, and ...

Increased levels of alpha-class and pi-class glutathione S-transferases in cell lines resistant to 1-chloro-2,4-dinitrobenzene ...

GSTK1 (Myc-DDK-tagged)-Human glutathione S-transferase kappa 1 (GSTK1), nuclear gene encoding mitochondrial protein, transcript variant 3 - 10 µg - OriGene - cdna clones

Cancer-associated somatic genome alterations offer great promise as cancer biomarkers. Here we describe a new biomarker for human prostate cancer: extensive methylation of deoxycytidine nucleotides distributed throughout a 5 CG island region of the pi-class glutathione S-transferase gene (GSTP1). Using the PCR to amplify a GSTP1 promoter sequence fragment containing 12 recognition sites for HpaII and MspI, 52 of 57 (91%) prostatic carcinoma DNA specimens demonstrated extensive somatic increases in deoxycytidine methylation, detected as amplification of target GSTP1 promoter sequences following HpaII digestion, but not following MspI treatment. Using nested primer sets, a sensitive PCR assay for extensive GSTP1 CG island methylation changes was developed that was capable of detecting 200 pg of prostate cancer cell DNA among 1 microgram of normal leukocyte DNA. This GSTP1 CG island DNA methylation assay, which targets a somatic genome change present in most prostate cancer cells but not in ...

Dive into the research topics of Regulatory roles of glutathione-S-transferases and 4-hydroxynonenal in stress-mediated signaling and toxicity. Together they form a unique fingerprint. ...

Gene silencing by epigenetic mechanisms is frequent in prostate cancer (PCA). The link between DNA hypermethylation and histone modifications is no...

Significance of the amyloidogenic transthyretin Val 122 Ile allele in African Americans in the arteriosclerosis risk in communities (ARIC) and cardiovascular health (CHS) studies ...

Abiotic stresses cause osmotic stress and ion toxicity that produces excessive ROS, these in turn cause oxidative stress in plants. GST is an abundant enzyme in plants encoded by an ancient and highly divergent gene superfamily with multiple functions that play active roles in the ROS scavenging pathways of plants. Previous studies have shown that overexpressing a GST gene can enhance the tolerance of transgenic plants to salt (Csiszár et al., 2014; Ji et al., 2010; Xu et al., 2015b; Zhang et al., 2018), cold (Huang et al., 2009; Roxas et al., 1997), drought (Ji et al., 2010; Lo Cicero et al., 2015; Xu et al., 2015b) and heavy metals (Dixit et al., 2011; Kumar et al., 2013); the common mechanism for the increased tolerance is reduction of oxidative damage. In previous studies, it has been widely shown that GSTs respond to salt stress and are also induced by other abiotic and biotic stresses; however, the function of GSTs in response to saline-alkali stresses were unknown. The plants were ...

Balchin, D., Stoychev, S.H, & Dirr, H.W. S-Nitrosation Destabilizes Glutathione Transferase P1-1 . Biochemistry. 52 (51), 9394-9402. 22/11/2013.. ...

Human GSTP1 full-length ORF ( AAH10915, 1 a.a. - 210 a.a.) recombinant protein with GST-tag at N-terminal. (H00002950-P01) - Products - Abnova

摘要(Abstract)： 目的探究GSTP1在不同阶段结肠癌组织中的表达水平,并查找可能对GSTP1表达产生调控作用的微小分子RNA(miR)及其对结肠肿瘤细胞的影响。方法利用实时定量PCR和蛋白印迹分析检测GSTP1在结肠癌组织中的表达水平;构建载体pcDNA3.1-GSTP1并转染到培养的人类结肠癌细胞系HCT116中,检测细胞的凋亡率与活性水平;利用TargetScan和MicroCosm ...

摘要(Abstract)： 为了研究丙二醛(MDA)对草鱼肠道、肝胰脏抗氧化防御能力的影响,以谷胱甘肽(GSH)/谷胱甘肽转移酶(GSTs)通路为研究对象,选择初始体重(74.8±1.0)g的草鱼(Ctenopharyngodon idelluspond),随机分为4组,每组设3个重复,每个重复20尾。4组草鱼分别投喂基础饲料(对照组)以及在基础饲料中添加61(B1组)、124(B2组)、185 mg/kg(B3组)MDA的试验饲料,在池塘网箱养殖72 d后,测定肠道、肝胰脏和血清中MDA和GSH含量,采用荧光定量PCR(qRT-PCR)方法测定草鱼肠道、肝胰脏GSH/GSTs通路中谷氨酸-半胱氨酸连接酶催化亚基(GCLC)、谷胱甘肽还原酶(GSR)、pi-谷胱甘肽硫转移酶(GSTpi)、微粒体谷胱甘肽硫转移酶1(MGSt1)基因表达量。结果显示:1)与对照组相比,除B3组肝胰脏MDA含量显著升高( ...

Morphologically, GSTP1−/− cells in growth-limiting conditions seemed to undergo marked cell death, with sporadic live cells that did not form colonies. To confirm morphologic apoptosis in the cells, we stained for pyknotic nuclei and calculated apoptotic indexes. We found no significant differences in apoptotic index in cells seeded at densities of 7.5 × 103 cells/cm2 ( Fig. 2B). However, starting with a lower seeding density of 2.0 × 103 cells/cm2, there was a 2-fold increase in the apoptotic index in GSTP1−/− cells compared with GSTP1+/+ cells, particularly after 8 days in culture. Furthermore, at the lowest seeding density of 0.5 × 103 cells/cm2, there was an up to 10-fold increase in the apoptotic index in GSTP1−/− cells compared with GSTP1+/+ cells.. Together, our findings show that GSTP1 is required for cell survival and proliferation in growth-limiting conditions. Notably, the differences in survival and growth at low-density seeding were not evident when the cells were ...

Handla b cker, sk nlitteratur och facklitteratur hos Adlibris bokhandel. Priserna ligger l gst p marknaden vilket har noterats i ett flertal test i media.

Handla b cker, sk nlitteratur och facklitteratur hos Adlibris bokhandel. Priserna ligger l gst p marknaden vilket har noterats i ett flertal test i media.

In May 1910, formed the first automobile company of the Russian army. Thus began a fascinating and dramatic history of domestic military vehicles. This story should not be pompous admire. It just need to know and love. How near and dear to you a man whose dignity and success command respect, and the disadvantages and problems - understanding ...

The genetic profile that is needed to define an endurance athlete has been studied during recent years. The main objective of this work is to approach for the first time the study of genetic variants in liver-metabolizing genes and their role in endurance performance by comparing the allelic and genotypic frequencies in elite endurance athletes to the non-athlete population. Genotypic and allelic frequencies were determined in 123 elite endurance athletes (75 professional road cyclists and 48 endurance elite runners) and 122 male non-athlete subjects (sedentary). Genotyping of cytochrome P450 family 2 subfamily D member 6 (CYP2D6 rs3892097), glutathione-S transferase mu isoform 1 (GSTM1), glutathione S-transferase pi (GSTP rs1695) and glutathione S-transferase theta (GSTT) genes was performed by polymerase chain reaction (PCR). The combination of the polymorphisms for the

At the end of ones tether with the NCBI database the following proteins were identified: isoform 1 of serum albumin (ALB1), HSP70, dihydropyrimidinase-related protein 2 (DPYSL2), isoforms of myelin root protein (MBP1), isoform 3 of spectrin alpha chain (SPTAN1), proton ATPase catalytic subunit A (ATP6V1A), glutathione S-transferase P (GSTP1), pro- tein DJ-1 (PUT7), and dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex (DLAT). Although subject to involuntary hydrolysis and detoxication away glutathione, successive 6 Target-Organ Toxicity: Liver and Kidney The using software is irritation version. WordPress: Free blogs managed by the developers of the WordPress package buy cabgolin 0.5 mg low price treatment arthritis. Chamber 31:11В-24 Montagna G, Cremona ML, Paris G, Amaya MF, Buschiazzo A, Alzari PM, Frasch ACC (2002) The trans-sialidase from the African trypanosome Trypanosoma brucei. This letter triggers the nomination of the Rapporteur and ...

J Agric Food Chem. 2009 Aug 3.. Liu AG, Volker SE, Jeffery EH, Erdman JW.. Division of Nutritional Sciences and Department of Food Science and Human Nutrition, 905 South Goodwin Avenue, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801.. Many studies have evaluated the cancer -preventive potential of individual bioactives from tomatoes and broccoli, but few have examined them within the context of a whole food. Male Copenhagen rats were fed diets containing 10% standard tomato powder, tomato enriched with lycopene or total carotenoids, standard broccoli floret, broccoli sprouts, or broccoli enriched with indole glucosinolates or selenium for 7 days. All broccoli diets increased the activity of colon quinone reductase (NQO1). Indole glucosinolate-enriched broccoli and selenium-enriched broccoli increased hepatic NQO1 and cytochrome P450 1A activity (P , 0.05). Standard broccoli and lycopene-enriched tomato diets down-regulated prostatic glutathione S-transferase P1 mRNA ...

The class of Omega glutathione transferases is newly identified with novel structural and functional characteristics. Human GSTO 1-1 (glutathione S-transferase Omega 1) is the first member of the GST Omega class. It was found to play a role in apoptosis and be in association with age-at-onset of AD and PD. In order to improve the understanding of the properties of other Omega class members, we screened a human fetal brain cDNA library and obtained the human GSTO2 (glutathione S-transferase Omega 2) cDNA. The full-length cDNA of human GSTO2 is 1179 bp long and encodes a protein of 243 amino acid residues. Expression pattern analysis revealed that GSTO2 was ubiquitously expressed at a low level, with a higher expression in pancreas and prostate. Enzyme assays showed that GSTO2 protein had activities similar to Omega class GSTs. It has detectable glutathione-dependent thiol transferase activity and glutathione-dependent dehydroascorbate reductase activity. But different from GSTO1-1, GSTO2 exhibits ...

In humans, glutathione-dependent conjugation of halomethanes is polymorphic, with 60% of the population classed as conjugators and 40% as non-conjugators. We report the characterization of the genetic polymorphism causing the phenotypic difference. We have isolated a cDNA that encodes a human class Theta GST (GSTT1) and which shares 82% sequence identity with rat class Theta GST5-5. From PCR and Southern blot analyses, it is shown that the GSTT1 gene is absent from 38% of the population. The presence or absence of the GSTT1 gene is coincident with the conjugator (GSST1+) and non-conjugator (GSTT1-) phenotypes respectively. The GSTT1+ phenotype can catalyse the glutathione conjugation of dichloromethane, a metabolic pathway which has been shown to be mutagenic in Salmonella typhimurium mutagenicity tester strains and is believed to be responsible for carcinogenicity of dichloromethane in the mouse. In humans, the enzyme is found in the erythrocyte and this may act as a detoxification sink. ...

Recombinant human GSTM5 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

KEGG Orthology (KO) [BR:adk00001] 09100 Metabolism 09106 Metabolism of other amino acids 00480 Glutathione metabolism [PATH:adk00480] Alide2_0050 Glutathione S-transferase domain protein K00799 GST; glutathione S-transferase [EC:2.5.1.18] 09180 Brite Hierarchies 09183 Protein families: signaling and cellular processes 02000 Transporters [BR:adk02000] Alide2_0050 Glutathione S-transferase domain protein K00799 GST; glutathione S-transferase [EC:2.5.1.18] Enzymes [BR:adk01000] 2. Transferases 2.5 Transferring alkyl or aryl groups, other than methyl groups 2.5.1 Transferring alkyl or aryl groups, other than methyl groups (only sub-subclass identified to date) 2.5.1.18 glutathione transferase Alide2_0050 Glutathione S-transferase domain protein K00799 GST; glutathione S-transferase [EC:2.5.1.18] Transporters [BR:adk02000] Other transporters Pores ion channels [TC:1] Alide2_0050 Glutathione S-transferase domain protein K00799 GST; glutathione S-transferase [EC:2.5.1.18 ...

Background: The glutathione S-transferase (GST) family of metabolising enzymes plays an important role in the detoxification of mutagens and carcinogens. The expression of many of these cancer susceptibility enzymes is genetically polymorphic. An increased frequency of GST-null genotypes has been associated with several malignancies. Objective: To investigate the rate of GSTT1 and GSTM1 null genotypes in AML patients and to determine its importance in prognosis of the disease. Methods: DNA was extracted by phenol/chloroform method from peripheral blood or bone marrow of 180 white Caucasian patients. A multiplex PCR method was used simultaneously to amplify regions of GSTM1, GSTT1, and b-globin genes in genomic DNA. The survival curves were analyzed by the Kaplan-Meier method and compared by the log-rank test (Mantel-Cox) using the SPSS software program. Results: Of the total of 180 patients, 23 cases (12.8%) showed null genotypes in both genes, while in 52 patients (28.9%) both genes were wild-types.

Addresses: Aceto A, Univ G DAnnunzio, Dipartimento Sci Biomed, Via Vestini 31, I-66100 Chieti, Italy. Univ G DAnnunzio, Dipartimento Sci Biomed, I-66100 Chieti, Italy. Univ Uppsala, Ctr Biomed, Dept Biochem, S-75123 Uppsala, Sweden.Available from: 2008-10-17 Created: 2008-10-17 Last updated: 2011-01-14 ...

Genetic polymorphisms of CYP2E1 and GSTP1 in a South Indian population--comparison with North Indians, Caucasians and Chinese.: CYP2E1 and GSTP1 enzymes belong

Summary: The glutathione S-transferase gene of Medicago sativa can effectively alleviate osmotic and oxidative damage induced by saline-alkali stress in transgenic tobacco. ...

sp:GSTM1_HUMAN] GSTM1, GST1, GSTM1-1, GSTM1a-1a, GSTM1b-1b, GTH4, GTM1, H-B, MU, MU-1; glutathione S-transferase mu 1; K00799 glutathione S-transferase [EC:2.5.1.18] ...

cfu:CFU_0016 K00799 glutathione S-transferase [EC:2.5.1.18] , (GenBank) gst; Glutathione S-transferase (A) MKLYYIPSACSLSPHIVANELGLPIELVKVDSKSKRTEHGDDYLAINPKGYVPALQLDDG RVLTEGPAVVQYLADLKPDAQLAPANGSMARYRLQEMLGYINSELHQGYLPLFYPETSDE MRAGRMAHLRKHYALIDATLGFTPFLLGERFSIADAYLFVVTRWATFVNLDLAPFPHLQA FQERIATRPAVQAALHREQAAAS ...

abh:M3Q_1880 K00799 glutathione S-transferase [EC:2.5.1.18] , (GenBank) putative glutathione S-transferase (A) MRVLYQFPLSHYCEKARWLLDHKELDYVAHNLIPGFHRAFAQLKTGQNLLPILKDDHRWI AESTKIALYLDDTYPEHALLRRDEQLRQQTLKIDSLADELGVHVRRWALAHTLAQGDHAL EIMMGEQGYLRQFEKISKPFLKTLVKKNYKLEEELVSQSKGCMDELINELNHYLIENQAR YMVGDRLSLADISVCSMLAPLLEIKGTPWEREEDGEVSPDWSNYQKYLLDLPLGQYVLRI YQTERNARVDWRGI ...

pep:known chromosome:VEGA66:3:108012255:108017973:-1 gene:OTTMUSG00000007188 transcript:OTTMUST00000016591 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:Gstm1 description:glutathione S-transferase, mu 1 ...