In cyanobacteria, nitrogen assimilation is a genuine photosynthetic process that requires ATP and reducing equivalents generated in the light. Both nitrate and nitrite are reduced to ammonium in the presence of photosynthetically reduced ferredoxin as the physiological electron donor. Ammonium is incorporated, through the glutamine synthetase-glutamate synthase pathway, into glutamate to yield glutamine by an ATP-dependent ligation reaction catalyzed by glutamine synthetase, and glutamate synthase transfers the amido group of glutamine to 2-oxoglutarate to regenerate glutamate in the presence of reduced ferredoxin. Nitrogen assimilation is tightly regulated in response to environmental cues. Nitrate and nitrite are taken up and reduced only in the absence of ammonium and under CO2 fixation conditions, and the level of glutamine synthetase protein is severely reduced in the presence of ammonium (12).. In the unicellular Synechococcus sp. strain PCC 7942, which does not fix molecular nitrogen, the ...
The SCOP classification for the Alpha subunit of glutamate synthase, C-terminal domain superfamily including the families contained in it. Additional information provided includes InterPro annotation (if available), Functional annotation, and SUPERFAMILY links to genome assignments, alignments, domain combinations, taxonomic visualisation and hidden Markov model information.
Garciarrubio, A., E. Lozoya, A. Covarrubias, F. Bolivar 1983. Structural organization of the genes that encode two glutamate synthase subunits of Escherichia coli. Gene 26:165- ...
American Eagle Instrument Curette Gracey 3/8 Rigid Ea - Model AEGA1-2RX : This product is being shipped directly to you from the manufacturer, and as
The primers used to make this knockout were TGTGAAGTCGGGTTTGTCTGCGCAGGCTATCAGTGAGATTTGACTGATTTATTCCGGGGATCCGTCGACC and CACTCATATAAAGAATAAGGTTTTTACAACCAAAAAAGAAGGTCGTTATGTGTAGGCTGGAGCTGCTTCG the bolded sequence matches the pKD13 ...
Hosted by the USGS Core Science Analytics and Synthesis. Page designed through the cooperative efforts of interagency ITIS Teams. Point of Contact: [email protected].
Note: The data shown is approximate and subject to standard industry and manufacturer tolerances. *Ampacities vary depending on the manufacturer and application. TRIPLEX SERVICE DROP. ...
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Põlismetsale iseloomulike tunnuste arenemine on seotud suktsessiooniga, mille viimase staadiumi saavutamine nõuab aega. Vanade puude akumulatsioon hakkab mõjutama puistu struktuuri. Vanemad puistud arendavad häilusid kahel põhjusel. Esiteks, dominantsed puud hakkavad surema loodusliku vananemisprotsessi tagajärjel, mis muudab puud patogeenidele ja kahjuritele vastuvõtlikumaks. Kui sellised vanad puud on koondunud, tekitab see küllaltki suuri häile[12]. Teiseks, mida vanem on puistu, seda enam on tõenäoline, et selle struktuuri on mõjutanud häiringud. Häiringud, nagu väiksemad põlengud ja tormid, üraskirüüsted, surmavad suuri vanu puid või nende gruppe.[13][14] Suurte puude suremise tulemusena kuhjub surnud puitu ja lagundavad seened satuvad ka elusatesse puudesse. Surnud ja surevad puud on põlismetsa iseloomulik tunnus ja need toetavad rikkalike toiduvõrgustike teket. Põlismetsadele on tavaliselt iseloomulikuks tunnuseks erivanuseline heterogeense struktuuriga kooslus; ...
Looking for online definition of glutamate synthase in the Medical Dictionary? glutamate synthase explanation free. What is glutamate synthase? Meaning of glutamate synthase medical term. What does glutamate synthase mean?
Bacterial NADPH-dependent glutamate synthase (GltS) is a complex iron-sulfur flavoprotein that catalyzes the reductive synthesis of two L-Glu molecules from L-Gln and 2-oxo-glutarate. GltS functional unit hosts an α-subunit (αGltS) and a β-subunit (βGltS) that assemble in different αβ oligomers in solution. Here, we present the cryo-electron microscopy structures of Azospirillum brasilense GltS in four different oligomeric states (αβ, αβ, αβ and αβ, in the 3.5- to 4.1-Å resolution range). Our study provides a comprehensive GltS model that details the inter-protomeric assemblies and allows unequivocal location of the FAD cofactor and of two electron transfer [4Fe-4S] clusters within βGltS ...
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Lamb, J.F.S., Samac, D.A., Barnes, D.K., Henjum, K.I. 2000. Increased herbage yield in alfalfa associated with selection for fibrous and lateral roots. Crop Science. 40:693-699. [pdf 108 k]. Schoenbeck, M.A., Temple, S.J., Trepp, G.B., Blumenthal, J.M., Samac, D.A., Gantt, J.S., Hernandez, G., Vance, C.P. 2000. Decreased NADH glutamate synthase activity in nodules and flowers of alfalfa (Medicago sativa L.) transformed with an antisense glutamate synthase transgene. Journal of Experimental Botany. 51(342):29-39. [pdf 407 k]. ...
Two pathways serve for assimilation of ammonia inParacoccus denitrificans. Glutamate dehydrogenase (NADP+) catalyzes the assimilation at a high NH4+ concentration. If nitrate serves as the nitrogen source, glutamate is synthesized by glutamate-ammonia ligase and glutamate synthase (NADPH). At a very low NH4+ concentration, all three enzymes are synthesized simultaneously. No direct relationship exists between glutamate dehydrogenase (NADP+) and glutamate-ammonia ligase inP. denitrificans, while the glutamate synthase (NADPH) activity changes in parallel with that of the latter enzyme. Ammonia does not influence the induction or repression of glutamate dehydrogenase (NADP+). The inner concentration of metabolites indicates a possible repression of glutamate dehydrogenase (NADP+) by the high concentration of glutamine or its metabolic products as in the case when NH4+ is formed by assimilative nitrate reduction. No direct effect of the intermediates of nitrate assimilation on the synthesis of glutamate
p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class=publication>Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href=http://www.nrbook.com/b/bookcpdf.php>Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...
Kanamori, Keiko and Weiss, Richard L. and Roberts, John D. (1988) Glutamate biosynthesis in Bacillus azotofixans. 15N NMR and enzymatic studies. Journal of Biological Chemistry, 263 (6). pp. 2817-2823. ISSN 0021-9258. https://resolver.caltech.edu/CaltechAUTHORS:KANjbc88 ...
Parim paik huvitavate inimestega kohtumiseks, kellega sul on sarnased huvid. Tee teste, tutvu uute inimestega, flirdi ning veeda lõbusalt aega ...
Lükopeen on mittemürgine ja toidus tavaline aine, kuid on teateid ülemäärasest lükopeeni tarvitamisest. Ühel keskealisel naisel, kes oli pikka aega joonud suures koguses tomatimahla, läksid nahk ja maks kollakasoranžiks ja tema vere lükopeenitase tõusis märgatavalt. Pärast kolmenädalast lükopeenivaba dieeti normaalne nahavärv taastus.[36] Seda nahavärvimuutust tuntakse lükopenodermiana[38] ja see ei ole tervistkahjustav. On ka lükopeenitalumatuse ja -allergia juhtumeid, millega võib kaasneda kõhulahtisus, iiveldus, kõhuvalu, kõhukrambid, soolegaasid, oksendamine ja isutus.[39] Karotenoidid, eriti lükopeen, arvatakse kõige tõhusamate singletse hapniku 1O2 kustutajate hulka. Viimane tekib näiteks fotokeemilistes reaktsioonides valguse neeldumisel ja ta on väga reaktiivne. Ta võib oksüdeerida mitmesuguseid aminohappeid valkudes, nukleiinhappeid ja küllastumata rasvhappeid. Kustutamisreaktsioonis lähevad karotenoidid üle ergastunud triplettolekusse (1O2 + 1Car → ...
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Summary: The influence of specific growth rate on the ammonia-assimilating enzymes glutamine synthetase, NADP-linked glutamate synthase and NADP-linked glutamate dehydrogenase was studied in ammonia-limited and glucose-limited chemostat cultures of Erwinia carotovora var. carotovora, E. nigrifluens and E. amylovora. The response of these organisms, representatives of various enzyme groups within the genus Erwinia, showed considerable variation and confirmed the heterogeneity of the genus with respect to ammonia-assimilating enzymes. The overall significance of the glutamine synthetase/glutamate synthase pathway in any organism where the synthesis of these enzymes is not constitutive is questioned.
NADH-GOGAT is a minor form of GOGAT species and little has been known concerning its cellular and subcellular localization, gene structure and expression, or function in higher plants. In 1992, a specific antibody for NADH-GOGAT from rice cell cultures was prepared as the first instance to crossreact NADH-GOGAT protein in rice plants.19 Antibody for NADH-GOGAT in alfalfa root nodules had been obtained prior to the rice, but the antibody hardly recognized NADH-GOGAT in plant organs other than nodules.20 Using the antibody for rice NADH-GOGAT, immunochemical and immunocyto-logical experiments have been performed. A high abundance of NADH-GOGAT protein was detected in the non-green, developing leaf blade14 and in the developing grains.21 Immunocytological results showed that the NADH-GOGAT protein was present in vascular parenchyma cells and mestome sheath cells of vascular bundles of the developing leaf blade, as well as in vascular parenchyma cells, nucellar projection, and nucellar epidermis of ...
Streptomyces lydicus partial gluS-alpha gene for NADPH glutamate synthase alpha subunit and partial gluS-beta gene for NADPH glutamate synthase beta subunit, strain NRRL 2433 Streptomyces lydicus partial gluS-alpha and partial gluS-beta genes, strain NRRL ...
ab Glutamate Dehydrogenase Detection Kit Instructions for Use For the rapid, sensitive and accurate measurement of Glutamate Dehydrogenase activity in various samples This product is for research
Data Availability StatementThe datasets used and/or analyzed through the current research are available through the corresponding writer on reasonable demand. SMDB11_RS09300 (GTP cyclohydrolase FolE2) was the most considerably upregulated having a log2 FC of 6.4; these DEGs had been enriched in various GO conditions, including hydrogen sulfide biosynthetic procedure, sulfur substance transmembrane transporter activity, and ABC transporter complicated. Additionally, many genes had been identified to make a difference genes in the PPI network, including SMDB11_RS17755 (upregulated; glutamate synthase huge subunit), SMDB11_RS00590 (upregulated; sulfite reductase subunit ), and SMDB11_RS04505 (upregulated; cystathionine -synthase). Therefore, SMDB11_RS09300, SMDB11_RS17755, SMDB11_RS00590, and SMDB11_RS04505 may play significant jobs in the antimicrobial level of resistance of by taking part in folate rate of metabolism or the integrity of cell membranes. Nevertheless, further experiments must ...
Ammonium (NH4+ + NH3) is the optimal nitrogen source for E. coli (i.e., the source which yields most rapid growth). Ammonium (pKa = 9.25) enters cells in two forms (Fig. 1). NH3, which is ∼2% of total ammonium at pH 7.4, crosses the cell membrane by unmediated diffusion, a process that cannot be altered genetically. When the pH is decreased to 5.5, NH3 is only ∼0.02% of total ammonium. NH4+, which is the bulk of total ammonium at both pH 7.4 and pH 5.5, can enter the cells if and only if the AmtB channel is expressed and functional. Its expression is controlled at the transcriptional level and regulated largely by the free-pool concentration of glutamine in the cell interior (8), whereas its activity is controlled by the regulatory protein GlnK, largely in response to the free-pool concentration of the precursor metabolite 2-oxoglutarate, which is an intermediate in the tricarboxylic acid cycle (ref. 11 and references cited therein). Expression of AmtB increases as the glutamine ...
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During the course of NH4+ (or NO2-)-plus-alpha-oxoglutarate-dependent O2 evolution in spinach (Spinacia oleracea) chloroplasts, glutamate was continuously excreted out of the chloroplasts. Under these conditions, for each molecule of NO2- or NH4+ which disappeared, one molecule of glutamate accumulated in the medium and the concentration of glutamate in the stroma space was maintained constant. SO4(2-) (or SO3(2-) behave as inhibitors of NH4+ incorporation into glutamate by intact chloroplasts. This considerable inhibition of glutamate synthesis by SO4(2-) was correlated with a rapid decline in the stromal Pi concentration. The reloading of stromal Pi with either external Pi or PPi4- relieved SO4(2-)-induced inhibition of glutamate synthesis by intact chloroplasts. It was concluded that SO4(2-) induced a rapid efflux of stromal Pi out of the chloroplast, leading to a limitation of ATP synthesis and therefore to an arrest of ATP-dependent glutamine synthetase functioning. ...
In this study aminotransferase inhibitors were used to determine the relative importance of different aminotransferases in providing nitrogen for de novo glutamate synthesis in the retina. Aminooxyacetate, which inhibits all aminotransferases, blocke
Glutamate dehydrogenase (GDH; EC 1.4.1.2-1.4.1.4) catalyses in vitro the reversible amination of 2-oxoglutarate to glutamate. In vascular plants the in vivo direction(s) of the GDH reaction and hence the physiological role(s) of this enzyme remain obscure. A phylogenetic analysis identified two clea …
Bacteria of the genus Azospirillum, including the most comprehensively studied Azospirillum brasilense, are non‐pathogenic soil bacteria that promote the growth of diverse plants, making them an attractive model to understand non‐symbiotic, beneficial plant‐bacteria associations
Comprises a multicomponent system, containing a reductase that is an iron-sulfur flavoprotein (FAD; EC 1.18.1.3), an iron-sulfur oxygenase, and ferredoxin ...
Glutamine Synthetase兔多克隆抗体(ab49873)可与小鼠, 大鼠, 猴样本反应并经WB, IHC,ICC/IF实验严格验证,被8篇文献引用并得到8个独立的用户反馈。
Glutamine Synthetase兔多克隆抗体(ab73593)可与小鼠, 大鼠, 狗, 人, 狨猴样本反应并经WB, IHC, ICC/IF实验严格验证,被3篇文献引用并得到8个独立的用户反馈。
a tagasisidest... tead, inimesed ei tule reeglina selle peale, et seda on vaja anda. eriti positiivset. Ma veetsin kevadel hulga aega veendumuses, et mu tantsuõpilased peavad mind ilgelt sitaks õpetajaks, sest kui nad midagi kommenteerisid, siis ainult seda, mida ma peaks teisiti tegema (noh, et õpeta seda rohkem või ma tahaks, et seda saaks rohkem korrata - või lihtsalt meeleheitehüüatused ma ei saa seda teha). mõtlesin täitsa tõsiselt, et lõpetan selle jama ära, ei ole mul vaja teistele ja endale kannatusi valmistada. Ja siis, kui ma sellest A.-ga rääkisin, tuli välja, et nurga taga temaga suitsu tehes räägivad nad talle küll, et küll ma olen hea tantsuõpetaja ja oh kuidas nende oskused on võrreldes varasemaga paremaks läinud. Nad ilmselt eeldasid, et sellest, et nad minust üldiselt hästi arvavad, saan ma isegi aru. et ma saan ise aru, et üldine foon on positiivne, lihtsalt vahel on mõni väike probleem ...
Kui valu tagasi: Mitu korda hermeetik ei kesta pikka aega.Pärast koristus, proovige panna nelk õli (tuntud ka kui küüs ekstrakt) langeb otse pessa ning seejärel pakendil, et paned pessa ala.Õli on loomulik lokaalanesteetikumiga ja aitab leevendada valu.Üle leti tooteid nagu orajel võib aidata veidi, kuid ei kõrvalda valu täielikult.Teine abinõu, mis võib aidata on panna cool jääpakile vastu mõjutas lõualuu.. ...
Mammals have three enzymes that can overcome the thermodynamic hurdles of ammonia assimilation: (i) carbamoyl phosphate synthetase I (CPS1), the adenosine triphosphate (ATP)-dependent, rate-limiting step of the urea cycle; (ii) glutamate dehydrogenase (GDH), a NADPH (reduced nicotinamide adenine dinucleotide phosphate)-dependent enzyme that catalyzes the reductive amination of α-ketoglutarate; and (iii) glutamine synthetase (GS), which catalyzes the ATP-dependent amination of glutamate to generate glutamine (12, 13) (fig. S1A). Analysis of transcriptomic data from The Cancer Genome Atlas for the ammonia-assimilating enzymes in healthy and cancerous tissues revealed that expression of GS and GDH mRNA is significantly increased across many cancer subtypes, whereas CPS1 mRNA is increased only in the colon (Fig. 1B). Among healthy tissues, GS and GDH are ubiquitously expressed and CPS1 is expressed only in the liver (fig. S1B). Breast cancers display increased expression of both GS and GDH. ...
Stephen J. Temple, Suman Bagga, Champa Sengupta-Gopalan; Can glutamine synthetase activity levels be modulated in transgenic plants by the use of recombinant DNA technology?. Biochem Soc Trans 1 November 1994; 22 (4): 915-920. doi: https://doi.org/10.1042/bst0220915. Download citation file:. ...
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The amino acid glutamate may be the principal excitatory transmitter in the nervous system including in sensory neurons that convey pain sensation from the periphery to the brain. tissue inflammation as mostly explored in transgenic mice. The possible interplay between glutamate biosynthesis and VGLUT-dependent packaging in synaptic vesicles and its potential impact in various pain states will be presented. PNU 200577 participation during SNI- incisional- and oxaliplatin-induced PNU 200577 pain) (see [30 31 2.3 VGLUT Deletion Visceral Pain and Itch As mentioned above virtually all colorectal and urinary bladder DRG neurons in mice express VGLUT2 and some also PNU 200577 VGLUT1 or VGLUT3 (Figure 1) suggesting that changes in their expression should modulate visceral pain. However global heterozygous VGLUT1- [40] or VGLUT2-KO mice [40] show no differences in their responses to the (rather nonspecific) acetic acid visceral pain test; comparison between global VGLUT3-KO mice and WT mice (same as ...
Glutamine synthetase, 0.1 ml. The protein encoded by this gene belongs to the glutamine synthetase family. It catalyzes the synthesis of glutamine from glutamate and ammonia.
Glutamine synthetase is an enzyme thats found in many tissues including the kidneys. The substance is used to help the body break down the chemical nitrogen. It also has the role of removing ammonia from the liver. These are important functions for the body.
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References for Abcams Mouse Glutamine Synthetase peptide (ab73592). Please let us know if you have used this product in your publication
Minu seekordseks vestluskaaslaseks on pikaaegne tippjuht ja mentor Maarika Liivamägi. Maarikal on mitmekülgne juhtimiskogemus erinevatest rahvusvahelistest organisatsioonidest (Statoil, SEB Bank, ERGO Insurance Grupp), kus ta on vastutanud müügi- ja turundusvaldkonna eest. Täna jagab Maarika oma aega ettevõtluse, ärikonsultatsioonide, Eesti Mentorite Koja ning Eesti Hotellide ja Restoranide liidu vahel. Istusime Maarikaga maha ja lobisesime juhtimisest, juhtidest ja üldse laiemalt, mida kõik need aastad kogemusi nii juhi kui ka mentorina on Maarikale õpetanud.. Üks asi, milleni ma aja ja kogemusega olen jõudnud, on see, et küll on hea olla sina ise. Kui ma vaatan näiteks oma mentorina toimetamist, siis ka sealt olen saanud üha rohkem kinnitust sellele, et me ei pea kedagi millekski või kellekski teiseks voolima või muutma - täitsa okei on olla sina ise. Küll aga peab sealjuures olema teadlik, kuidas sa oma käekirja ja stiiliga nii iseendale kui ka ülejäänud ...
Kuidas tõhusalt oma harjumusi muuta? Külas on eratreener Rauno Oolberg, kes võttis hiljuti 20 kg kaalus juurde ja siis need jälle maha - lihtsalt selleks, et omal nahal tunda, mida tähendab muuta oma harjumusi ning mõista, kuidas seda tõhusamalt teha. Tema kõrval on saates kliiniline psühholoog Kaia Kuppart, kes selgitab, kuidas meie keha uute harjumustega kohaneb ja kui kaua võtab aega, et uuest tegevusest saaks elu loomulik osa. Saadet juhib Tervisekassa avalike suhete peaspetsialist Heidi Kukk.. ...
Definition of assimilation in the Legal Dictionary - by Free online English dictionary and encyclopedia. What is assimilation? Meaning of assimilation as a legal term. What does assimilation mean in law?