Porphyromonas gingivalis is the foremost oral pathogen of adult periodontitis in humans. However, the mechanisms of bacterial invasion and the resultant destruction of the gingival tissue remain largely undefined. We report host-P. gingivalis interactions in primary human gingival fibroblast (HGF) cells. Quantitative immunostaining revealed the need for a high multiplicity of infection for optimal infection. Early in infection (2-12 h), P. gingivalis activated the proinflammatory transcription factor NF-kappa B, partly via the PI3 kinase/AKT pathway. This was accompanied by the induction of cellular anti-apoptotic genes, including Bfl-1, Boo, Bcl-XL, Bcl2, Mcl-1, Bcl-w and Survivin. Late in infection (24-36 h) the anti-apoptotic genes largely shut down and the pro-apoptotic genes, including Nip3, Hrk, Bak, Bik, Bok, Bax, Bad, Bim and Moap-1, were activated. Apoptosis was characterized by nuclear DNA degradation and activation of caspases-3, -6, -7 and -9 via the intrinsic mitochondrial pathway. Use of
Porphyromonas gingivalis is the foremost oral pathogen of adult periodontitis in humans. However, the mechanisms of bacterial invasion and the resultant destruction of the gingival tissue remain largely undefined. We report host-P. gingivalis interactions in primary human gingival fibroblast (HGF) cells. Quantitative immunostaining revealed the need for a high multiplicity of infection for optimal infection. Early in infection (2-12 h), P. gingivalis activated the proinflammatory transcription factor NF-kappa B, partly via the PI3 kinase/AKT pathway. This was accompanied by the induction of cellular anti-apoptotic genes, including Bfl-1, Boo, Bcl-XL, Bcl2, Mcl-1, Bcl-w and Survivin. Late in infection (24-36 h) the anti-apoptotic genes largely shut down and the pro-apoptotic genes, including Nip3, Hrk, Bak, Bik, Bok, Bax, Bad, Bim and Moap-1, were activated. Apoptosis was characterized by nuclear DNA degradation and activation of caspases-3, -6, -7 and -9 via the intrinsic mitochondrial pathway. Use of
BACKGROUND AND AIM: An important goal of periodontal plastic surgery is the creation of attached gingiva around the teeth. In this study, the aims were to culture gingival fibroblasts in a biodegradable scaffold and measure the width of attached gingiva after the clinical procedure.METHODS: This study was carried out on 4 patients (8 sites), with inadequate attached gingiva next to at least two teeth in contralateral quadrants of the same jaw. A biopsy of attached gingiva (epithelial + connective tissue) was taken using a surgical blade. Following culture of gingival fibroblasts, 250 × 103 cells in 250 µl nutritional medium were mixed with platelet-rich in growth factor (PRGF). Periosteal fenestration technique was done on one side (control) and tissue-engineered mucosal graft (test) was carried out on the contralateral side in each patient. The width of keratinized tissue, probing depth (PD) and width of attached gingiva were recorded at baseline and 3 months after the operation.RESULTS: An increased
Nitric oxide synthase type-II is synthesized by human gingival tissue and cultured human gingival fibroblasts.: Nitric oxide is known to be an important inflamm
Gomez-Florit, Manuel; Ramis, Joana Maria; Xing, Rui; Taxt-Lamolle, Sebastien Francis Michel; Haugen, Håvard Jostein; Lyngstadaas, Ståle Petter & Monjo Cabrer, Marta (2014). Differential response of human gingival fibroblasts to titanium- and titanium-zirconium-modified surfaces. Journal of Periodontal Research. ISSN 0022-3484. 49(4), s 425- 436 . doi: 10.1111/jre.12121 Vis sammendrag Background and Objective: Gingival fibroblasts are responsible for the constant adaptation, wound healing and regeneration of gingival connective tissue. New titanium-zirconium (TiZr) abutment surfaces have been designed to improve soft tissue integration and reduce implant failure compared with titanium (Ti). The aim of the present study was firstly to characterize a primary human gingival fibroblasts (HGF) model and secondly to evaluate their differential response to Ti and TiZr polished (P), machined (M), and machined + acid-etched (modMA) surfaces, respectively. Material and Methods: HGF were cultured on ...
PubMed comprises more than 30 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
In periodontal therapy, the use of low-level diode lasers has recently been considered to improve wound healing of the gingival tissue. However, its effects on human gingival epithelial cells (HGECs)...
Alpha-melanocyte stimulating hormone (α-MSH) is involved in normal skin wound healing and also has anti-inflammatory properties. The association of α-MSH to polyelectrolyte layers with various supports has been shown to improve these anti-inflammatory properties. This study aimed to evaluate the effects of nanofibrous membrane functionalized with α-MSH linked to polyelectrolyte layers on gingival cell inflammatory response. Human oral epithelial cells (EC) and fibroblasts (FB) were cultured on plastic or electrospun Poly-#-caprolactone (PCL) membranes with α-MSH covalently coupled to Poly-L-glutamic acid (PGA-α-MSH), for 6 to 24 h. Cells were incubated with or without Porphyromonas gingivalis lipopolysaccharide (Pg-LPS). Cell proliferation and migration were determined using AlamarBlue test and scratch assay. Expression of interleukin-6 (IL-6), tumor necrosis factor (TNF-α), and transforming growth factor-beta (TGF-β) was evaluated using RT-qPCR method. Cell cultures on plastic showed that PGA-α
Irsogladine maleate (IM) counters Aggregatibacter actinomycetemcomitans-induced reduction of the gap junction intercellular communication and the expression of zonula occludens-1, which is a major tight junction structured protein, in cultured human gingival epithelial cells (HGEC). In addition, IM obviates the A. actinomycetemcomitans-induced increase in interleukin (IL)-8 levels in HGEC. Thus, by regulating the intercellular junctional complex and chemokine secretion in HGEC, IM may be useful to prevent periodontal disease. To clarify the effects and regulatory mechanism of IM in vivo and in vitro, we examined the expression of E-cadherin and neutrophil chemotaxis induced by A. actinomycetemcomitans under IM pretreatment. Immunohistochemical studies revealed that A. actinomycetemcomitans application to the gingival sulcus decreased the number of cells positive for E-cadherin and increased those positive for cytokine-induced neutrophil chemoattractant-2α (CINC-2α) in rat gingival epithelium ...
Preventive Effect of Daiokanzoto TJ-84 on 5-Fluorouracil-Induced Human Gingival Cell Death through the Inhibition of Reactive Oxygen Species Production. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
DOI: 10.11607/prd.00.0824 Cultured gingival dermal substitute (CGDS), composed of gingival fibroblasts and matrix and fabricated using tissue-engineering techniques, has been used for root coverage procedures. Fourteen sites from four patients with ≥ 2 mm of Miller Class I or II facial gingival tissue recession were treated. The autologous CGDS sheet, prepared prior to surgical treatment, was grafted over the teeth with gingival recession and then covered with a coronally positioned flap. Vertical and horizontal recession was measured at baseline (prior to the surgical procedure) and 13 to 40 weeks (average: 30.7 9.6 weeks) after surgery. The average vertical and horizontal root coverage after surgery was 79.1% 25.7% and 75.2% 31.4%, respectively. Moreover, there was a significant increase of keratinized and attached gingival tissue at the final clinical evaluation compared with preoperative measurements (P < .05). These results demonstrate CGDS as a promising grafting material for use with ...
in Journal of Periodontal Research (1999), 34(6), 323-8. Several grafting techniques and guided tissue regeneration techniques (GTR) have been well-developed in periodontal surgery. However, these techniques could induce pain and side effects, such as a ... [more ▼]. Several grafting techniques and guided tissue regeneration techniques (GTR) have been well-developed in periodontal surgery. However, these techniques could induce pain and side effects, such as a gingival recession during the healing period following the therapy. The graft of a small autologous connective tissue, using non-invasive surgical techniques could yield several benefits for the patients. Our preliminary study explores the feasibility of collecting healthy gingival tissues, culturing them in vitro to amplify rat gingival fibroblasts (RGF) and inoculating the obtained cells into autologous rat gingival tissues in vivo. Gingival tissues samples were cultured as explants as described by Freshney et al. and Adolphe. ...
Objective: To investigate the contribution of cyclooxygenase-1 (COX-1) and cyclooxygenase-2 (COX-2) to PGE2 production in human gingival fibroblast cells stimulated with lipopolysaccharide from periodontopathogenic bacteria. Methods: Lipopolysaccharide was isolated from Porphyromonas gingivalis and Prevotella intermedia by the phenol-water method. Human gingival fibroblast cells were stimulated with lipopolysaccharide in different concentration for different time periods. The levels of PGE2 in the culture medium were measured by radioimmunoassay. The expression of COX-2 protein was observed by immunocytochemical staining; COX-1 and COX-2 mRNA expression were examined by semi-quantitative RT-PCR analysis. Results: The production of PGE2 in human gingival fibroblast cells was increased in a time- and dose-dependent manner (P < .01, P < .01). Immunocytochemical staining showed the positive expression of COX-2 protein in stimulated cells around the unstained cell nucleus, while no expression was ...
C57BL/6-GFP Mouse Gingival Epithelial Cells from Creative Bioarray are isolated from C57BL/6-GFP-Tg(CAG-EGFP)1Osb/J mouse gingival tissue of pathogen-free laboratory mice. C57BL/6-GFP Mouse Gingival Epithelial Cells are grown in a T25 tissue culture flask pre-coated with gelatin-based coating solution for 2 min and incubated in Creative Bioarrays Culture Complete Growth Medium for 3-5 days. Cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 0.5x10^6 cells per ml and is delivered frozen. These cells are pre-coated with EpCAM-1 (CD326) antibody, following the application of magnetic beads pre-coated with secondary antibody Cells can be expanded for 3-7 passages at a split ratio of 1:2 under the cell culture conditions specified by Creative Bioarray. Repeated freezing and thawing of cells is not recommended ...
Hamster Gingival Epithelial Cells from Creative Bioarray are isolated from gingival tissue of pathogen-free laboratory mice. Hamster Gingival Epithelial Cells are grown in a T25 tissue culture flask pre-coated with gelatin-based coating solution for 2 min and incubated in Creative Bioarrays Culture Complete Growth Medium for 3-5 days. Cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 0.5x10^6 cells per ml and is delivered frozen. Cells can be expanded for 3-7 passages at a split ratio of 1:2 under the cell culture conditions specified by Creative Bioarray. Repeated freezing and thawing of cells is not recommended ...
Objective: Several local factors can affect the wound-healing process, delaying its progression and postponing tissue homeostasis. It is known that local inflammation is related to wound healing; however, the maintenance of the inflammatory reaction can impair the proliferation and migration of oral mucosal cells. The aim of this study was to evaluate the viability and chemokine expression of epithelial cells and gingival fibroblasts exposed to long-term lipopolysaccharide (LPS) treatment. Design: Epithelial cells (HaCaT, Cell Lines Service, 300493) and human gingival fibroblasts (HGFs) were seeded (1 105 cells/well) in 24-well plates and incubated for 24 h. To simulate the responses of cells to a local chronic oral mucosal inflammation, we added LPS of Escherichia coli (10 mg/ml) to Dulbeccos modified Eagles medium (DMEM), kept in contact with fibroblasts and epithelial cells for 24, 48, and 72 h. Then the cells were assessed for viability (alamarBlue assay), number (trypan blue assay), and ...
Periodontitis is a chronic inflammatory disease affecting the soft tissue and bone that surrounds the teeth. Despite extensive research, distinctive genes responsible for the disease have not been identified. The objective of this study was to elucidate transcriptome changes in periodontitis, by investigating gene expression profiles in gingival tissue obtained from periodontitis-affected and healthy gingiva from the same patient, using RNA-sequencing. Gingival biopsies were obtained from a disease-affected and a healthy site from each of 10 individuals diagnosed with periodontitis. Enrichment analysis performed among uniquely expressed genes for the periodontitis-affected and healthy tissues revealed several regulated pathways indicative of inflammation for the periodontitis-affected condition. Hierarchical clustering of the sequenced biopsies demonstrated clustering according to the degree of inflammation, as observed histologically in the biopsies, rather than clustering at the individual ...
Epithelial mesenchymal transition (EMT) has been implicated in neoplastic invasion and metastasis. We have previously generated six cell lines from human gingival mucosal keratinocytes immortalized by E6/E7 of human papillomavirus type 16. Ldk and NuB1 lines represented EMT phenotype and other four lines represented cobblestone non-EMT phenotype. These cell lines were utilized as model cells to determine whether inhibitors of apoptosis proteins and cell-cycle regulators were molecular players during EMT. EMT cells exhibited increased expression of vimentin, vascular endothelial growth factor (VEGF) receptor1 and the ability to form tubules on Matrigel as well as to grow anchorage independently. We found that EMT cells expressed significantly elevated levels of cIAP-1, Bclx and p27 kip higher than non-EMT cells. These proteins could therefore be used as intrinsic indicators of EMT of human gingival keratinocytes. © 2009 John Wiley & Sons A/S ...
Background and Aim: Previous studies have shown that some dyes used in photodynamic therapy (PDT) have a low pH and may cause root demineralization and remove the smear layer. This study aimed to assess and compare the effects of root biomodification with citric acid and PDT on the adhesion and proliferation ...
Fibroblasts are quality tested in FibroLife® S2 or FibroLife® Xeno-Free Medium to ensure proper growth and morphology for at least 15 population doublings.
Background: Evidences have shown that local anaesthetics are clinically useful compounds that exert a pharmacological effect by blocking nerve impulse propagation and also it is able to provoke proliferation and cell growth. Aims: The aim of this study w...
UCL Discovery is UCLs open access repository, showcasing and providing access to UCL research outputs from all UCL disciplines.
Direct bonding of orthodontic brackets is an established procedure and has lead to increasing number of light-cured bonding materials produced with ever-increasing bond strength and ease of delivery. However, considerably less attention is given to biocompatibility of these materials. Both in vivo and in vitro studies indicate orthodontic bonding agents are cytotoxic. Spectrophotometric analysis revealed that these resins contained a great amount of potentially toxic, leachable, un-polymerized material, even when fully cured. Hypothesis: Different light-cured bonding agents may have different cytotoxic effects depending on their chemistry. Objective: Compare and analyze the toxicity of three different light-cured bonding agents to a population of gingival cells as measured by cell growth, and viability in vitro. Method: Light Bond®, MonoLok®, and Transbond XT® orthodontic adhesives were tested. Human gingival cells were obtained from extracted third molars, pooled, and expanded until needed. ...
THEODORE ROSETT, PAMELA S. GARNER, and LOUIS P. GANGAROSA Department of Biochemistry, Temple University School of Dentistry, Philadelphia, Pennsylvania, 19140, USA and Department of Oral Biology, Medical College of Georgia, Augusta, Georgia J Dent Res May-June 1972, Vol 51 No. 3 Previous studies (RoSETT ET AL, J Dent Res 50:161, 1971; GANGAROSA and ROSETT, J Dent Res 50:163, 1971) and the present one aim to determine physiologic and metabolic processes in human gingival tissue to discover how these processes are altered during the induction of periodontal disease. We chose cattle gingival epithelium to have a readily available supply of tissues. In this study we investigated the normal levels of some enzymes involved in glucose metabolism within bovine attached gingival epithelium. In determining the relative levels of the enzyme in any particular series of metabolic pathways, it is emphasized that we are only reporting the potential for conversion of substrate to product. The existence of these ...
Dental professionals are well positioned to educate patients about the overall health implications of chronic inflammation, particularly relating to periodontal disease. A greater understanding of the role of inflammation in various seemingly unrelated conditions allows practitioners to provide alternative strategies for prevention while resolving inflammation to reduce the risk of various systemic conditions.2. Periodontitis occurs when bacteria trigger inflammation with the release of various biochemical mediators, such as cytokines, which may lead to the destruction of gingival connective tissue and bone.3 Chronic adult periodontitis is the common form of this bacterially induced inflammatory disease, which affects a substantial number of adults and has shown to be a significant contributing factor to other systemic conditions.4 Chronic diseases such as atherosclerotic cardiovascular disease, rheumatoid arthritis, and diabetes mellitus may develop or worsen in part due to uncontrolled ...
Unscramble gingival, Unscramble letters gingival, Point value for gingival, Word Decoder for gingival, Word generator using the letters gingival, Word Solver gingival, Possible Scrabble words with gingival, Anagram of gingival
TY - JOUR. T1 - Biofilm-stimulated epithelium modulates the inflammatory responses in co-cultured immune cells. AU - Brown, Jason L.. AU - Johnston, William. AU - Delaney, Chris. AU - Rajendran, Ranjith AU - Butcher, John. AU - Khan, Shaz. AU - Bradshaw, David. AU - Ramage, Gordon. AU - Culshaw, Shauna. N1 - Acceptance from webpage OA article. PY - 2019/10/31. Y1 - 2019/10/31. N2 - The gingival epithelium is a physical and immunological barrier to the microbiota of the oral cavity, which interact through soluble mediators with the immune cells that patrol the tissue at the gingival epithelium. We sought to develop a three-dimensional gingivae-biofilm interface model using a commercially available gingival epithelium to study the tissue inflammatory response to oral biofilms associated with "health", "gingivitis" and "periodontitis.". These biofilms were developed by sequential addition of microorganisms to mimic the formation of supra- and sub-gingival plaque in vivo. Secondly, to mimic the ...
Nadine Krockow. The aim of this literature review was to define the most favorable surface topography and macrodesign of the transmucosal zone of abutments to achieve optimal soft tissue seal. A search identified 12 articles-3 human studies, 3 animal studies, and 6 in vitro studies-meeting the inclusion criteria for final evaluation. The human histologic studies showed that laser-ablated, hydrophilic, and oxidized titanium surfaces result in perpendicular insertion of human gingival fibroblasts into the treated abutment surface. Epithelial cells seem to slightly favor zirconia and polished titanium surfaces.. 2018 January/February; 66(1):18-25. ...
Titanium tetrafluoride solutions: occlusion of dentinal tubules and citotoxicity on gingival fibroblasts. Scholarships in Brazil Scientific Initiation. Eliza Maximiano Cury. Health Sciences
409 Role of Neutrophils in Periodontal Disease 051205_lecture32 Artigo DENGUE In Adv Nutr 2011 Wallace 1 7 Osteoarthritis Interleukin-6 and Tumor Necrosis Factor-A Production After Acute Psychological Cytotoxicity of Resin Monomers on Human Gingival Fib Rob Lasts and HaCaT Keratinocytes Cell Communication Biology Tooth Movement Interluekin The Effects of Ursolic Acid on Cytokine Production Sepsis.pptx
The keratinized oral gingival epithelium provides effective protection against both mechanical trauma and bacterial invasion. The horny cell layer acts like a film of plastic wrap, allowing the body to retain moisture and protecting it from invasion by foreign substances and comprises various substances such as keratins, produced by the epidermal keratinocytes and lipids. The epidermal keratinocytes divide in the basal layer, produce keratins and differentiate, and migrate to the upper layers as they mature by a process called keratinization. The present review discusses the structure of keratin, the process of keratinization and their distribution in gingival epithelium.. ...
This study indicates that the deficient immunomodulatory function of iGMSCs could be rescued by ASA pretreatment via upregulating of FasL in mice. This strategy might serve as a practical approach to rescue deficient MSC function for further therapeutic application.
Advanced search is divided into two main parts, and one or more groups in each of the main parts. The main parts are the "Search for" (including) and the "Remove from search" (excluding) part. (The excluding part might not be visible until you hit "NOT" for the first time ...
Principal Investigator:SAITO Kihachi, Project Period (FY):1989 - 1990, Research Category:Grant-in-Aid for General Scientific Research (C), Research Field:Functional basic dentistry
Affiliation:愛知学院大学,歯学部,講師, Research Field:矯正・小児・社会系歯学,小児・社会系歯学, Keywords:歯周疾患,小児,細胞増殖能,b-FGF,サイトカイン,PDGF-AB,Down Syndrome,gingival fibroblasts,growth activity,歯肉線維芽細胞, # of Research Projects:7, # of Research Products:0
Dont take Seretide without checking for Side Effects | A female patient was diagnosed with asthma, gingival disorder, treated with SERETIDE and reported asthmatic crisis,gingival disorder,gingival pain. Dosage: .
Learn about the causes, symptoms, diagnosis & treatment of Periodontal Disorders from the Professional Version of the Merck Manuals.
TY - JOUR. T1 - Human gingival fibroblast feeder cells promote maturation of induced pluripotent stem cells into cardiomyocytes. AU - Matsuda, Yusuke. AU - Takahashi, Ken. AU - Kamioka, Hiroshi. AU - Naruse, Keiji. PY - 2018/9/10. Y1 - 2018/9/10. N2 - The use of human induced pluripotent stem (iPS) cells has been investigated in multiple regenerative medicine studies. However, although methods for efficient differentiation of iPS cells into heart tissues have been devised, it remains difficult to obtain cardiac tissue with high contractility. Herein, we established a method for differentiating iPS cells into highly contractile cardiomyocytes (CMs), and demonstrate that the use of human gingival fibroblasts (HGFs) as a feeder cells promotes maturation of iPS-derived CMs (iPS-CMs) in vitro. After CM differentiation of iPS cells, iPS-CMs showed increased mRNA expression of the CM specific maker cardiac troponin T (cTnT) in the absence and presence (on-feeder condition) of cocultured HGFs, and ...
Shikonin, an active ingredient of Lithospermum erythrorhizon, exerts anti-inflammatory and antibacterial effects, and promotes wound healing. We investigated whether shikonin stimulated gingival tissue wound healing in human gingival fibroblasts (hGF). In addition, we evaluated the effects of shikonin on the mitogen-activated protein kinase (MAPK) signaling pathway, which has an important role in wound healing. hGF were subjected to primary culture using gingiva collected from patients. The cells were exposed to/treated with Shikonin at concentrations ranging from 0.01 to 100 μM. The optimal concentration was determined by cell proliferation and migration assays. Type I collagen and fibronectin synthesis, the gene expression of vascular endothelial growth factor (VEGF) and FN, and the phosphorylation of Extracellular signal-regulated kinase (ERK) 1/2 were investigated. Identical experiments were performed in the presence of PD98059 our data suggest, a specific ERK 1/2 inhibitor. Shikonin
Looking for online definition of gingival mucosa in the Medical Dictionary? gingival mucosa explanation free. What is gingival mucosa? Meaning of gingival mucosa medical term. What does gingival mucosa mean?
Two novel proteins - odontogenic ameloblast-associated protein and amelotin - have recently been identified in maturation-stage ameloblasts and in the junctional epithelium. This article reviews the structure and function of the junctional epithelium, the pattern of expression of odontogenic ameloblast-associated and amelotin proteins and the potential involvement of these proteins in the formation and regeneration of the junctional epithelium. ...
Porphyromonas gingivalis, a periodontal pathogen, can invade primary cultures of gingival epithelial cells. Optimal invasion occurred at a relatively low multiplicity of infection (i.e., 100) and demonstrated saturation at a higher multiplicity of infection. Following the lag phase, during which bacteria invaded poorly, invasion was independent of growth phase. P. gingivalis was capable of replicating within the epithelial cells. Invasion was an active process requiring both bacterial and epithelial cell energy production. Invasion was sensitive to inhibitors of microfilaments and microtubules, demonstrating that epithelial cell cytoskeletal rearrangements are involved in bacterial entry. P. gingivalis, but not epithelial cell, protein synthesis was necessary for invasion. Invasion within the epithelial cells was not blocked by inhibitors of protein kinase activity. Invasion was inhibited by protease inhibitors, suggesting that P. gingivalis proteases may be involved in the invasion process. ...
OBJECTIVES The purpose of the present study was to analyse transcriptomes and mRNA expression levels for specific genes in calcium-channel blocker-induced gingival overgrowth (GO) tissues. DESIGN Eight gingival tissues samples (from both GO negative and positive sites) were harvested from four GO patients for microarray analyses. Twelve candidate genes were selected for further quantitative real time reverse transcription-polymerase chain reaction (qRT-PCR) analyses. Ten GO tissues from periodontitis patients and ten control gingival tissues from healthy subjects were compared by qRT-PCR. Mann-Whitney U-test was used for statistical evaluation. RESULTS In GO positive tissues, 163-1631 up-regulated and 100-695 down-regulated genes were identified with more than two-fold changes compared with GO negative tissues amongst patients by microarray experiments. No commonly expressed genes amongst the eight sets of microarray data were found. The clustering analysis confirmed that the entire transcriptome
Monkey periodontal ligament fibroblasts (MPLF cells), human gingival fibroblasts (HGF cells), rat embryonic calvaria cells (REC cells), porcine periodontal ligament epithelial cells (PPLE cells) and rat osteosarcoma 17/2 cells (ROS cells) were incorporated into 3-dimensional collagen gels plated in 60 mm Petri dishes in order: first, to measure the capacity of these cell types to contract; second, to investigate cell-collagen and intercellular relationships during contraction; and third, to define the cellular contribution to tissue contraction in an in vitro system. Measurements at times up to 72 h on 3 ml gels containing 5 × 10(5) cells and with a collagen concentration of 1.20 mg/ml showed that MPLF cells contracted the gels at a significantly greater rate (P less than 0.001) than did the other cell types. In addition, contraction started sooner and was of greater extent than with the other cells. HGF cells contracted the gels more rapidly than REC and PPLE cells, while ROS cells caused no ...
Background: Root surface debridement (RSD) is necessary to create an environment suitable for reattachment of the periodontium. Root surface conditioning may aid the formation of a biocompatible surface suitable for cell reattachment. BioPure™ MTAD (mixture of Doxycycline, citric acid and a detergent) is an endodontic irrigant with antibacterial properties and the ability to remove smear layer. It was hypothesized that MTAD may be useful for root surface conditioning. The efficacy of MTAD as a conditioner was measured by examining fibroblast attachment to root surfaces. Materials and Methods: Thirty-two specimens of human teeth with advanced periodontal disease were used. The surfaces were root planed until smooth. Half of the specimens were treated with 0.9% saline and the other samples with Biopure MTAD. As a negative control group, five further samples were left unscaled with surface calculus. Human gingival fibroblast cells HGF1-PI1 were cultured and poured over the tooth specimens and ...
The etiopathogenesis of periodontal disease results from complex interaction between infectious agents, mainly including bacteria, and host cellular and humoral immune responses. However it is thought that bacteria-induced pathogenesis is not sufficient alone to explain all biological and clinical features of the destructive periodontal disease. The main hypothesis is that herpesviruses, such as Epstein-Barr Virus, may participate as well by altering epithelial gingival cell biology and consequently may promote the initiation and progression of periodontitis ...
Gingival And Periodontal Pocket. Gingival and periodontal pockets are dental terms indicating the presence of an abnormal gingival sulcus near the point at which the gums contact a tooth. ...
Arlington Heights oral surgeon, Dr. Khan recommends gingival graft if a build-up of calculus and plaque on the root or if tooth decay has already started.
In the most drastic stage of periodontal disease, the bone and the attachment of the gums to the teeth have been destroyed. This may cause your teeth to shift or loosen and can affect how your teeth come together. You may notice a bad taste or smell in your mouth. Proper dental care must be initiated to save the teeth or they may need to be removed. Professional intervention may involve pocket reduction therapy and bone grafting along with the incorporation of other therapies.. Pocket reduction therapy is required for this form of periodontal disease when the gingival tissues have not resolved after initial treatment or tissue and root therapy. This is usually necessary when gingival tissues have not shrunk enough or when the supporting bone around the teeth has been lost. Since the gum tissue has not shrunk, they provide a greater place for bacteria to live and attack the bone and tissue causing further damage to occur.. Pocket reduction is a periodontal disease therapy that turns or pulls back ...
Periodontitis is a chronic destructive category of periodontal disease that progresses to the resorption of alveolar bone, which leads to progressive bone destruction and tooth loss. As a consequence of resorption, breakdown of products are released into periodontal tissues, migrating toward the gingival sulcus and gathering from the surrounding site in whole saliva, where several of them have been identified. 2930. Among the several host enzymes proposed as diagnostic indicators of periodontal status, ALP was one of the first to be identified. 31 ALP is released from polymorphonuclear cells (PMNs) during inflammation 10 and from osteoblasts 32 and periodontal ligament fibroblasts 33 during bone formation and periodontal regeneration respectively.. ALP activity in serum has been extensively studied, and it was suggested that ALP allows bone mineralization by releasing an organic phosphate that contributes to the deposition of calcium phosphate complexes into the osteoid matrix. 3435 ALP might ...